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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | ○ | Article | Enhanced Tumor Imaging Using Glucosamine-Conjugated Polyacrylic Acid-Coated Ultrasmall Gadolinium Oxide Nanoparticles in Magnetic Resonance Imaging | Owing to a higher demand for glucosamine (GlcN) in metabolic processes in tumor cells than in normal cells (i.e., GlcN effects), tumor imaging in magnetic resonance imaging (MRI) can be highly improved using GlcN-conjugated MRI contrast agents. Here, GlcN was conjugated with polyacrylic acid (PAA)-coated ultrasmall gadolinium oxide nanoparticles (UGONs) (d(avg) = 1.76 nm). Higher positive (brighter or T-1) contrast enhancements at various organs including tumor site were observed in human brain glioma (U87MG) tumor-bearing mice after the intravenous injection of GlcN-PAA-UGONs into their tail veins, compared with those obtained with PAA-UGONs as control, which were rapidly excreted through the bladder. Importantly, the contrast enhancements of the GlcN-PAA-UGONs with respect to those of the PAA-UGONs were the highest in the tumor site owing to GlcN effects. These results demonstrated that GlcN-PAA-UGONs can serve as excellent T-1 MRI contrast agents in tumor imaging via GlcN effects. | Liu, Shuwen; Yue, Huan; Ho, Son Long; Kim, Soyeon; Park, Ji Ae; Tegafaw, Tirusew; Ahmad, Mohammad Yaseen; Kim, Seungho; Saidi, Abdullah Khamis Ali Al; Zhao, Dejun; Liu, Ying; Nam, Sung-Wook; Chae, Kwon Seok; Chang, Yongmin; Lee, Gang Ho | Kyungpook Natl Univ, Coll Nat Sci, Dept Chem, Taegu 41566, South Korea; Korea Inst Radiol & Med Sci KIRAMS, Div RI Convergence Res, Seoul 01817, South Korea; Kyungpook Natl Univ, Sch Med, Dept Mol Med, Taegu 41944, South Korea; Kyungpook Natl Univ, Teachers Coll, Dept Biol Educ, Taegu 41566, South Korea | Nam, Sung-Wook/V-5519-2019; Ho, Son Long/P-3183-2015; Ahmad, Mohammad/AAH-2164-2020 | 57208926248; 57200329016; 55659242700; 57203773250; 16319690600; 55983618600; 57203054570; 57218284791; 57217492867; 57222567792; 57221731093; 16167127700; 15743626400; 7501840633; 7404851841 | liushuwen0701@gmail.com;yuehuan888@gmail.com;sonlongh@gmail.com;ksy0188@kirams.re.kr;jpark@kirams.re.kr;tirukorea@gmail.com;yaseen.knu@gmail.com;kseungho5335@gmail.com;abdullah_al_saidi@hotmail.com;djzhao.chem@gmail.com;ly1124161@gmail.com;nams@knu.ac.kr;kschae@knu.ac.kr;ychang@knu.ac.kr;ghlee@mail.knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 3 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.53 | 2025-06-25 | 7 | 7 | glucosamine; enhanced tumor imaging; GlcN effects; ultrasmall gadolinium oxide nanoparticle; magnetic resonance imaging | INFRARED FLUORESCENT-PROBES; SOFT ACIDS; BASES HSAB; HARD; ANGIOGENESIS; NANOCARRIERS; TRANSPORTERS; RELAXATION; CLEARANCE; PARTICLES | Enhanced tumor imaging; GlcN effects; Glucosamine; Magnetic resonance imaging; Ultrasmall gadolinium oxide nanoparticle | Acrylic Resins; Animals; Contrast Media; Gadolinium; Glucosamine; Magnetic Resonance Imaging; Mice; Nanoparticles; Neoplasms; glucosamine; nanoparticle; polyacrylic acid; acrylic acid resin; carbopol 940; contrast medium; gadolinium; gadolinium oxide; glucosamine; nanoparticle; animal experiment; Article; cell viability assay; coating (procedure); contrast enhancement; controlled study; crystal structure; cytotoxicity; energy dispersive X ray spectroscopy; Fourier transform infrared spectroscopy; hydrophobicity; inductively coupled plasma atomic emission spectrometry; mouse; neoplasm; nonhuman; nuclear magnetic resonance imaging; photon correlation spectroscopy; physical chemistry; relaxation time; signal noise ratio; surface property; T1 weighted imaging; T2 weighted imaging; thermogravimetry; transmission electron microscopy; X ray diffraction; zeta potential; animal; neoplasm; nuclear magnetic resonance imaging; procedures | English | 2022 | 2022-02 | 10.3390/ijms23031792 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Enhancing the Expression of the OsF3H Gene in Oryza sativa Leads to the Regulation of Multiple Biosynthetic Pathways and Transcriptomic Changes That Influence Insect Resistance | The white-backed planthopper (WBPH) is a major pest of rice crops and causes severe loss of yield. We previously developed the WBPH-resistant rice cultivar "OxF3H" by overexpressing the OsF3H gene. Although there was a higher accumulation of the flavonoids kaempferol (Kr) and quercetin (Qu) as well as salicylic acid (SA) in OxF3H transgenic (OsF3H or Trans) plants compared to the wild type (WT), it is still unclear how OsF3H overexpression affects these WBPH resistant-related changes in gene expression in OxF3H plants. In this study, we analyze RNA-seq data from OxF3H and WT at several points (0 h, 3 h, 12 h, and 24 h) after WBPH infection to explain how overall changes in gene expression happen in these two cultivars. RT-qPCR further validated a number of the genes. Results revealed that the highest number of DEGs (4735) between the two genotypes was detected after 24 h of infection. Interestingly, it was found that several of the DEGs between the WT and OsF3H under control conditions were also differentially expressed in OsF3H in response to WBPH infestation. These results indicate that significant differences in gene expression between the "OxF3H" and "WT" exist as the infection time increases. Many of these DEGs were related to oxidoreductase activity, response to stress, salicylic acid biosynthesis, metabolic process, defense response to pathogen, cellular response to toxic substance, and regulation of hormone levels. Moreover, genes involved in salicylic acid (SA) and ethylene (Et) biosynthesis were upregulated in OxF3H plants, while jasmonic acid (JA), brassinosteroid (Br), and abscisic acid (ABA) signaling pathways were found downregulated in OxF3H plants during WBPH infestation. Interestingly, many DEGs related to pathogenesis, such as OsPR1, OsPR1b, OsNPR1, OsNPR3, and OsNPR5, were found to be significantly upregulated in OxF3H plants. Additionally, genes related to the MAPKs pathway and about 30 WRKY genes involved in different pathways were upregulated in OxF3H plants after WBPH infestation. This suggests that overexpression of the OxF3H gene leads to multiple transcriptomic changes and impacts plant hormones and pathogenic-related and secondary-metabolites-related genes, enhancing the plant's resistance to WBPH infestation. | Jan, Rahmatullah; Asaf, Sajjad; Lubna, Saleem; Asif, Saleem; Kim, Eun-Gyeong; Jang, Yoon-Hee; Kim, Nari; Al-Harrasi, Ahmed; Lee, Gang-Seob; Kim, Kyung-Min | Kyungpook Natl Univ, Grad Sch, Dept Appl Biosci, Daegu 41566, South Korea; Kyungpook Natl Univ, Coastal Agr Res Inst, Daegu 41566, South Korea; Univ Nizwa, Nat & Med Sci Res Ctr, Nizwa 611, Oman; Abdul Wali Khan Univ, Dept Bot, Garden Campus, Mardan 23200, Pakistan; Natl Inst Agr Sci, Dept Agr Biotechnol, Rural Dev Adm, Jeonju 54874, South Korea | Jan, Rahmatullah/AIC-3439-2022; Asaf, Sajjad/ABA-3647-2021; Kim, Kyung-Min Kim/C-7007-2014; Ul-Hamid, Anwar/B-7297-2015 | 57201981969; 56595059900; 57200621537; 57396413700; 57221496070; 57219901992; 57395985700; 6506093146; 25927158200; 34868260300 | kangslee@korea.kr;kkm@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 23 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.71 | 2025-06-25 | 8 | 8 | Oryza sativa; pathogen; defense system; salicylic acid; jasmonic acid; transcriptomics | SYSTEMIC ACQUIRED-RESISTANCE; ABSCISIC-ACID; ETHYLENE-RESPONSE; SOGATELLA-FURCIFERA; DEFENSE RESPONSES; PLANT-RESISTANCE; SALICYLIC-ACID; JASMONIC ACID; NILAPARVATA-LUGENS; DISEASE RESISTANCE | defense system; jasmonic acid; Oryza sativa; pathogen; salicylic acid; transcriptomics | Animals; Biosynthetic Pathways; Gene Expression Regulation, Plant; Insecta; Oryza; Plant Diseases; Plants, Genetically Modified; Salicylic Acid; abscisic acid; brassinosteroid; ethylene; jasmonic acid; mitogen activated protein kinase; oxidoreductase; peroxiredoxin 1; phytohormone; salicylic acid; toxic substance; transcription factor; Article; bioinformatics; biosynthesis; controlled study; cultivar; differential gene expression; gene expression; gene ontology; gene overexpression; genotype; high throughput sequencing; hormone determination; infestation; insect resistance; nonhuman; pathogenesis; pathway analysis; physiological stress; plant defense; planthopper; protein expression; real time polymerase chain reaction; real time reverse transcription polymerase chain reaction; rice; RNA extraction; RNA sequencing; signal transduction; transcriptomics; upregulation; wild type; animal; biosynthesis; gene expression regulation; genetics; insect; metabolism; Oryza; plant disease; transgenic plant | English | 2022 | 2022-12 | 10.3390/ijms232315308 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Exfoliation and Reassembly Routes to a Ge/RuO2 Nanocomposite as an Anode for Advanced Lithium-Ion Batteries | Ge/RuO2 nanocomposites were successfully fabricated as anode materials for lithium-ion batteries using RuO2 nanosheets and Ge/GeO2 nanoparticles (NPs). X-ray diffraction (XRD) and X-ray absorption spectroscopy (XAS) analyses showed that elemental Ge nanoparticles were distributed onto the rutile-type RuO2. Transmission electron microscopy images showed well-dispersed Ge nanoparticles embedded in rutile-type RuO2. The Ge/RuO2 nanocomposite maintained higher discharge capacities (471 mA h g(-1)) after the 90th cycle at 0.1 A g(-1) than that (211 mA h g(-1)) of Ge/GeO2 nanoparticles. The Ge/RuO2 nanocomposite exhibited a higher capacity retention than Ge/GeO2 NPs. These results suggest that the well-dispersed Ge nanoparticles within RuO2 matrices enhance the cycle stability and capacity retention of the anode material. | Jang, Jeong-Hun; Lee, Minseop; Koo, Ji-Hye; Paek, Seung-Min | Kyungpook Natl Univ, Dept Chem, Daegu 41566, South Korea | Paek, Seung-Min/E-4667-2011; Lee, Minseop/JVZ-7552-2024 | 57198251792; 57201550392; 57221701800; 7102686289 | smpaek@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 23 | 19 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.44 | 2025-06-25 | 5 | 6 | germanium; ruthenium oxide; anode material; lithium-ion battery | COMPOSITE; NANOSHEETS; STORAGE; RUO2 | anode material; germanium; lithium-ion battery; ruthenium oxide | Electric Power Supplies; Electrodes; Ions; Lithium; Nanocomposites; Titanium; germanium; lithium ion; nanosheet; titanium dioxide; ion; lithium; nanocomposite; titanium; titanium dioxide; Article; controlled study; transmission electron microscopy; X ray absorption spectroscopy; X ray diffraction; chemistry; electrode; power supply | English | 2022 | 2022-10 | 10.3390/ijms231911766 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Exogenously Applied Sodium Nitroprusside Mitigates Lead Toxicity in Rice by Regulating Antioxidants and Metal Stress-Related Transcripts | Sustainable agriculture is increasingly being put in danger by environmental contamination with dangerous heavy metals (HMs), especially lead (Pb). Plants have developed a sophisticated mechanism for nitric oxide (NO) production and signaling to regulate hazardous effects of abiotic factors, including HMs. In the current study, we investigated the role of exogenously applied sodium nitroprusside (SNP, a nitric oxide (NO) donor) in ameliorating the toxic effects of lead (Pb) on rice. For this purpose, plants were subjected to 1.2 mM Pb alone and in combination with 100 mu M SNP. We found that under 1.2 mM Pb stress conditions, the accumulation of oxidative stress markers, including hydrogen peroxide (H2O2) (37%), superoxide anion (O-2(-)) (28%), malondialdehyde (MDA) (33%), and electrolyte leakage (EL) (34%), was significantly reduced via the application of 100 mu M SNP. On the other hand, under the said stress of Pb, the activity of the reactive oxygen species (ROS) scavengers such as polyphenol oxidase (PPO) (60%), peroxidase (POD) (28%), catalase (CAT) (26%), superoxide dismutase (SOD) (42%), and ascorbate peroxidase (APX) (58%) was significantly increased via the application of 100 mu M SNP. In addition, the application of 100 mu M SNP rescued agronomic traits such as plant height (24%), number of tillers per plant (40%), and visible green pigments (44%) when the plants were exposed to 1.2 mM Pb stress. Furthermore, after exposure to 1.2 mM Pb stress, the expression of the heavy-metal stress-related genes OsPCS1 (44%), OsPCS2 (74%), OsMTP1 (83%), OsMTP5 (53%), OsMT-I-1a (31%), and OsMT-I-1b (24%) was significantly enhanced via the application of 100 mu M SNP. Overall, our research evaluates that exogenously applied 100 mM SNP protects rice plants from the oxidative damage brought on by 1.2 mM Pb stress by lowering oxidative stress markers, enhancing the antioxidant system and the transcript accumulation of HMs stress-related genes. | Rahim, Waqas; Khan, Murtaza; Al Azzawi, Tiba Nazar Ibrahim; Pande, Anjali; Methela, Nusrat Jahan; Ali, Sajid; Imran, Muhammad; Lee, Da-Sol; Lee, Geun-Mo; Mun, Bong-Gyu; Moon, Yong-Sun; Lee, In-Jung; Yun, Byung-Wook | Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea; Yeungnam Univ, Dept Hort & Life Sci, Gyongsan 38541, South Korea | ; Pande, Anjali/ABH-2333-2021; Imran, Muhammad/AFL-6590-2022; Ali, Sajid/GLS-7322-2022; Mun, BongGyu/GYD-6010-2022; Lee, In-Jung/GLS-0432-2022 | 57431774700; 57207990116; 57224980187; 57222624154; 57880065700; 57214290889; 58282433800; 57222624235; 57222627507; 57147241300; 7203054923; 16425830900; 8245123600 | bwyun@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 17 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 3.36 | 2025-06-25 | 33 | 40 | nitric oxide; antioxidants; metal-stress related transcripts; rice; Pb-stress | NITRIC-OXIDE; COPPER TOXICITY; INDIAN MUSTARD; CADMIUM; ACCUMULATION; OXYGEN; CD; DETOXIFICATION; L.; EXPRESSION | antioxidants; metal-stress related transcripts; nitric oxide; Pb-stress; rice | antioxidant; ascorbate peroxidase; catalase; catechol oxidase; hydrogen peroxide; lead; malonaldehyde; nitroprusside sodium; peroxidase; pigment; reactive oxygen metabolite; superoxide dismutase; agronomic trait; Article; bioaccumulation; controlled study; gene expression; genetic marker; genetic transcription; nonhuman; oxidative stress; plant height; plant stress; rice; single nucleotide polymorphism | English | 2022 | 2022-09 | 10.3390/ijms23179729 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Generative Adversarial Networks for Creating Synthetic Nucleic Acid Sequences of Cat Genome | Nucleic acids are the basic units of deoxyribonucleic acid (DNA) sequencing. Every organism demonstrates different DNA sequences with specific nucleotides. It reveals the genetic information carried by a particular DNA segment. Nucleic acid sequencing expresses the evolutionary changes among organisms and revolutionizes disease diagnosis in animals. This paper proposes a generative adversarial networks (GAN) model to create synthetic nucleic acid sequences of the cat genome tuned to exhibit specific desired properties. We obtained the raw sequence data from Illumina next generation sequencing. Various data preprocessing steps were performed using Cutadapt and DADA2 tools. The processed data were fed to the GAN model that was designed following the architecture of Wasserstein GAN with gradient penalty (WGAN-GP). We introduced a predictor and an evaluator in our proposed GAN model to tune the synthetic sequences to acquire certain realistic properties. The predictor was built for extracting samples with a promoter sequence, and the evaluator was built for filtering samples that scored high for motif-matching. The filtered samples were then passed to the discriminator. We evaluated our model based on multiple metrics and demonstrated outputs for latent interpolation, latent complementation, and motif-matching. Evaluation results showed our proposed GAN model achieved 93.7% correlation with the original data and produced significant outcomes as compared to existing models for sequence generation. | Hazra, Debapriya; Kim, Mi-Ryung; Byun, Yung-Cheol | Jeju Natl Univ, Dept Comp Engn, Jeju 63243, South Korea; Kyungpook Natl Univ, Vet Internal Med, Daegu 41566, South Korea; Jeju Natl Univ, Inst Informat Sci & Technol, Dept Comp Engn, Jeju 63243, South Korea | ; Kim, Dong Ki/J-5389-2012 | 57191375984; 59446102700; 8897891700 | debapriyah@jejunu.ac.kr;karimaker@hanmail.net;ycb@jejunu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 7 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 1.77 | 2025-06-25 | 18 | 20 | synthetic genome; generative adversarial networks; nucleic acid sequences; WGAN-GP; cat genome; promoter prediction; promoter classification; motif matching | cat genome; generative adversarial networks; motif matching; nucleic acid sequences; promoter classification; promoter prediction; synthetic genome; WGAN-GP | Adenosine Deaminase; DNA; Image Processing, Computer-Assisted; Intercellular Signaling Peptides and Proteins; nucleic acid; adenosine deaminase; DNA; signal peptide; Article; cat; DNA sequence; filtration; genome; high throughput sequencing; illumina sequencing; nonhuman; nucleotide sequence; outcome assessment; promoter region; image processing; procedures | English | 2022 | 2022-04 | 10.3390/ijms23073701 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Genome and Transcriptome-Wide Analysis of OsWRKY and OsNAC Gene Families in Oryza sativa and Their Response to White-Backed Planthopper Infestation | Plants are threatened by a wide variety of herbivorous insect assaults, and display a variety of inherent and induced defenses that shield them against herbivore attacks. Looking at the massive damage caused by the white-backed planthopper (WBPH), Sogatella furcifera, we undertook a study to identify and functionally annotate OsWRKY and OsNAC transcription factors (TFs) in rice, especially their involvement in WBPH stress. OsWRKY and OsNAC TFs are involved in various developmental processes and responses to biotic and abiotic stresses. However, no comprehensive reports are available on the specific phycological functions of most of the OsWRKY and OsNAC genes in rice during WBPH infestation. The current study aimed to comprehensively explore the OsWRKY and OsNAC genes by analyzing their phylogenetic relationships, subcellular localizations, exon-intron arrangements, conserved motif identities, chromosomal allocations, interaction networks and differential gene expressions during stress conditions. Comparative phylogenetic trees of 101 OsWRKY with 72 AtWRKY genes, and 121 OsNAC with 110 AtNAC genes were constructed to study relationships among these TFs across species. Phylogenetic relationships classified OsWRKY and OsNAC into eight and nine clades, respectively. Most TFs in the same clade had similar genomic features that represented similar functions, and had a high degree of co-expression. Some OsWRKYs (Os09g0417800 (OsWRKY62), Os11g0117600 (OsWRKY50), Os11g0117400 (OsWRKY104) and OsNACs (Os05g0442700, Os12g0630800, Os01g0862800 and Os12g0156100)) showed significantly higher expressions under WBPH infestation, based on transcriptome datasets. This study provides valuable information and clues about predicting the potential roles of OsWRKYs and OsNACs in rice, by combining their genome-wide characterization, expression profiling, protein-protein interactions and gene expressions under WBPH stress. These findings may require additional investigation to understand their metabolic and expression processes, and to develop rice cultivars that are resistant to WBPH. | Khan, Ibrahim; Jan, Rahmatullah; Asaf, Sajjad; Khan, Abdul Latif; Bilal, Saqib; Kim, Kyung-Min; Al-Harrasi, Ahmed | Univ Nizwa, Nat & Med Sci Res Ctr, Nizwa 616, Oman; Kyungpook Natl Univ, Coll Agr & Life Sci, Sch Appl Biosci, Div Plant Biosci, Daegu 41566, South Korea; Univ Houston, Dept Engn Technol, Sugar Land, TX 77479 USA | Ul-Hamid, Anwar/B-7297-2015; Asaf, Sajjad/ABA-3647-2021; Jan, Rahmatullah/AIC-3439-2022; Khan, Abdul/H-5910-2011; Kim, Kyung-Min Kim/C-7007-2014; Khan, Ibrahim/JMC-5061-2023 | 57191379139; 57201981969; 56595059900; 26639372800; 57031617400; 34868260300; 6506093146 | kkm@knu.ac.kr;aharrasi@unizwa.edu.om; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 23 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.44 | 2025-06-25 | 5 | 5 | biotic stress; WBPH; transcription factors; OsWRKY; OsNAC; phylogeny; genomics | DISEASE RESISTANCE; OVER-EXPRESSION; BIOTIC STRESS; RICE | biotic stress; genomics; OsNAC; OsWRKY; phylogeny; transcription factors; WBPH | Animals; Gene Expression Profiling; Hemiptera; Herbivory; Oryza; Phylogeny; Arabidopsis; Article; biotic stress; cellular distribution; cladistics; conserved sequence; controlled study; differential expression analysis; differential gene expression; exon; gene duplication; gene expression; gene expression profiling; gene location; gene ontology; gene structure; genome analysis; infestation; intron; nac gene; nonhuman; orthology; phylogenetic tree; phylogeny; plant chromosome; plant gene; plant genome; planthopper; protein interaction; protein protein interaction; rice; transcriptomics; white backed planthopper; wrky gene; animal; genetics; Hemiptera; herbivory; metabolism; Oryza | English | 2022 | 2022-12 | 10.3390/ijms232315396 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Genome-Wide Identification of Aquaporin Genes in Adzuki Bean (Vigna angularis) and Expression Analysis under Drought Stress | The adzuki bean Vigna angularis (Wild.) is an important leguminous crop cultivated mainly for food purposes in Asian countries; it represents a source of carbohydrates, digestible proteins, minerals, and vitamins. Aquaporins (AQPs) are crucial membrane proteins involved in the transmembrane diffusion of water and small solutes in all living organisms, including plants. In this study, we used the whole genome sequence of the adzuki bean for in silico analysis to comprehensively identify 40 Vigna angularis aquaporin (VaAQP) genes and reveal how these plants react to drought stress. VaAQPs were compared with AQPs from other closely-related leguminous plants, and the results showed that mustard (Brassica rapa) (59), barrel medic (Medicago truncatula) (46), soybean (Glycine max) (66), and common bean (Phaseolus vulgaris L.) (41) had more AQP genes. Phylogenetic analysis revealed that forty VaAQPs belong to five subfamilies, with the VaPIPs (fifteen) subfamily the largest, followed by the VaNIPs (ten), VaTIPs (ten), VaSIPs (three), and VaXIPs (two) subfamilies. Furthermore, all AQP subcellular locations were found at the plasma membrane, and intron-exon analysis revealed a relationship between the intron number and gene expression, duplication, evolution, and diversity. Among the six motifs identified, motifs one, two, five, and six were prevalent in VaTIP, VaNIP, VaPIP, and VaXIP, while motifs one, three, and four were not observed in VaPIP1-3 and VaPIP1-4. Under drought stress, two of the VaAQPs (VaPIP2-1 and VaPIP2-5) showed significantly higher expression in the root tissue while the other two genes (VaPIP1-1 and VaPIP1-7) displayed variable expression in leaf tissue. This finding revealed that the selected VaAQPs might have unique molecular functions linked with the uptake of water under drought stress or in the exertion of osmoregulation to transport particular substrates rather than water to protect plants from drought. This study presents the first thorough investigation of VaAQPs in adzuki beans, and it reveals the transport mechanisms and related physiological processes that may be utilized for the development of drought-tolerant adzuki bean cultivars. | Tayade, Rupesh; Rana, Varnika; Shafiqul, Mohammad; Nabi, Rizwana Begum Syed; Raturi, Gaurav; Dhar, Hena; Thakral, Vandana; Kim, Yoonha | Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea; Natl Agrifood Biotechnol Inst NABI, Mohali 140306, India; Natl Inst Crop Sci, Rural Dev Adm, Dept Southern Area Crop Sci, Miryang 50424, South Korea | Rana, Varnika/HKW-6801-2023; Dhar, Hena/AFI-8381-2022; Raturi, Gaurav/AAZ-9646-2021; Tayade, Rupesh/AAM-9652-2021 | 57191753234; 59863232300; 58026504400; 57200232212; 57203205942; 24830132600; 57221468067; 57224866763 | kyh1229@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 24 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.88 | 2025-06-25 | 7 | 10 | aquaporin gene; drought; adzuki bean; genome-wide identification; gene structure; phylogeny | PLASMA-MEMBRANE AQUAPORINS; PLANT AQUAPORINS; WATER; SILICON; TRANSPORT; FAMILY; PERMEABILITY; SELECTIVITY; CHANNELS; PROTEIN | adzuki bean; aquaporin gene; drought; gene structure; genome-wide identification; phylogeny | Aquaporins; Droughts; Phaseolus; Phylogeny; Vigna; Water; aquaporin; chlorophyll; plant RNA; transcriptome; water; aquaporin; adzuki bean; amino acid sequence; Arabidopsis; Article; barrel medic; Brassica rapa; cell membrane; cell vacuole; cellular distribution; chlorophyll content; chromosome 1; chromosome 10; chromosome 11; chromosome 2; chromosome 5; chromosome 6; chromosome 7; chromosome 8; chromosome 9; computer model; conserved sequence; drought stress; drought tolerance; dry weight; exon; fresh weight; gene expression; gene expression profiling; gene sequence; gene structure; genome-wide association study; hydraulic conductivity; intron; nonhuman; osmoregulation; Phaseolus vulgaris; phylogeny; plant genome; plant height; plant leaf; plant root; protein tertiary structure; real time polymerase chain reaction; rice; RNA extraction; sequence alignment; shoot; soil moisture; soybean; water transport; drought; genetics; metabolism; Phaseolus; phylogeny; Vigna | English | 2022 | 2022-12 | 10.3390/ijms232416189 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Hemp-Derived Nanovesicles Protect Leaky Gut and Liver Injury in Dextran Sodium Sulfate-Induced Colitis | Hemp (Cannabis sativa L.) is used for medicinal purposes owing to its anti-inflammatory and antioxidant activities. We evaluated the protective effect of nanovesicles isolated from hemp plant parts (root, seed, hemp sprout, and leaf) in dextran sulfate sodium (DSS)-induced colitis in mice. The particle sizes of root-derived nanovesicles (RNVs), seed-derived nanovesicles (SNVs), hemp sprout-derived nanovesicles (HSNVs), and leaf-derived nanovesicles (LNVs) were within the range of 100-200 nm as measured by nanoparticle tracking analysis. Acute colitis was induced in C57BL/N mice by 5% DSS in water provided for 7 days. RNVs were administered orally once a day, leading to the recovery of both the small intestine and colon lengths. RNVs, SNVs, and HSNVs restored the tight (ZO-1, claudin-4, occludin) and adherent junctions (E-cadherin and alpha-tubulin) in DSS-induced small intestine and colon injury. Additionally, RNVs markedly reduced NF-kappa B activation and oxidative stress proteins in DSS-induced small intestine and colon injury. Tight junction protein expression and epithelial cell permeability were elevated in RNV-, SNV-, and HSNV-treated T84 colon cells exposed to 2% DSS. Interestedly, RNVs, SNVs, HSNVs, and LNVs reduced ALT activity and liver regeneration marker proteins in DSS-induced liver injury. These results showed for the first time that hemp-derived nanovesicles (HNVs) exhibited a protective effect on DSS-induced gut leaky and liver injury through the gut-liver axis by inhibiting oxidative stress marker proteins. | Eom, Jung-Young; Choi, Sang-Hun; Kim, Hyun-Jin; Kim, Dong-ha; Bae, Ju-Hyun; Kwon, Gi-Seok; Lee, Dong-hee; Hwang, Jin-Hyeon; Kim, Do-Kyun; Baek, Moon-Chang; Cho, Young-Eun | Andong Natl Univ, Dept Food & Nutr, Andong 36729, South Korea; Kyungpook Natl Univ, Sch Med, Cell & Matrix Res Inst, Dept Mol Med, Daegu 41944, South Korea; Andong Natl Univ, Dept Hort & Med Plant, Andong 36729, South Korea; Andong Natl Univ, Ind Acad Cooperat Fdn, Andong 36729, South Korea; Jeonbuk Natl Univ, Korea Zoonosis Res Inst, Iksan 54531, South Korea | Kim, Hyun-Jin/AAJ-2905-2021; Choi, Sanghun/AGS-7430-2022; kim, david/AAA-4537-2020; Kim, Dongha/HNO-9659-2023 | 57225930425; 57225948184; 57225935531; 57219014701; 57225046295; 16947529800; 57880544100; 57219012305; 57442742600; 7006013097; 56390104900 | mcbaek@knu.ac.kr;yecho@anu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 17 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 1.77 | 2025-06-25 | 19 | 20 | inflammatory bowel disease; hemp-derived nanovesicles; oxidative stress proteins; tight junction; gut-liver axis | INFLAMMATORY-BOWEL-DISEASE; EXOSOME-LIKE NANOPARTICLES; NF-KAPPA-B; INTESTINAL BARRIER; PERMEABILITY; CANNABIDIOL; CELLS; PLANT | gut-liver axis; hemp-derived nanovesicles; inflammatory bowel disease; oxidative stress proteins; tight junction | 3 nitrotyrosine; alanine aminotransferase; alpha tubulin; aspartate aminotransferase; claudin 4; cyclin D1; cyclooxygenase 2; dextran sulfate; endothelial nitric oxide synthase; hemp derived nanovesicle; I kappa B; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; nanoparticle; nitric oxide; occludin; phytochemical; protein ZO1; tumor necrosis factor; unclassified drug; uvomorulin; alanine aminotransferase blood level; animal cell; animal experiment; animal model; animal tissue; Article; aspartate aminotransferase blood level; Cannabis sativa; cell membrane permeability; controlled study; dextran sulfate sodium-induced acute colitis; disease activity score; drug isolation; enzyme activity; human; human cell; intestine epithelium cell; intestine injury; intestine mucosa permeability; liver injury; liver protection; liver regeneration; male; mouse; nonhuman; oxidative stress; particle size; plant leaf; plant root; plant seed; protein expression; sprout; T84 cell line | English | 2022 | 2022-09 | 10.3390/ijms23179955 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Hypoxia Promotes Angiogenic Effect in Extracranial Arteriovenous Malformation Endothelial Cells | Arteriovenous malformation (AVM) is characterized by high-flow blood vessels connecting arteries and veins without capillaries. This disease shows increased angiogenesis and a pathophysiological hypoxic environment in proximal tissues. Here, we analyzed the effects of hypoxia on angiogenesis in the endothelial cells (ECs) of AVM and normal tissues. ECs from human normal and AVM tissues were evaluated using immunocytochemistry with CD31. In vitro tube formation under hypoxia was tested in both ECs using Matrigel. The relative expression of angiogenesis-related genes was measured using real-time PCR. Under normoxia, CD31 was significantly higher in AVM ECs (79.23 +/- 0.65%) than in normal ECs (74.15 +/- 0.70%). Similar results were observed under hypoxia in AVM ECs (63.85 +/- 1.84%) and normal ECs (60.52 +/- 0.51%). In the tube formation test under normoxic and hypoxic conditions, the junction count and total vessel length were significantly greater in AVM ECs than normal ECs. Under both normoxia and hypoxia, the angiogenesis-related gene FSTL1 showed a significantly higher expression in AVM ECs than in normal ECs. Under hypoxia, CSPG4 expression was significantly lower in AVM ECs than in normal ECs. Accordingly, the angiogenic effect was increased in AVM ECs compared with that in normal ECs. These results provide a basic knowledge for an AVM treatment strategy. | Lee, Joon Seok; Cho, Hyun Geun; Ryu, Jeong Yeop; Oh, Eun Jung; Kim, Hyun Mi; Kwak, Suin; Lee, Seok-Jong; Lee, Jongmin; Lee, Sang Yub; Huh, Seung; Kim, Ji Yoon; Chung, Ho Yun | Kyungpook Natl Univ, Sch Med, Dept Plast & Reconstruct Surg, Daegu 41944, South Korea; Kyungpook Natl Univ, Sch Med, Cell & Matrix Res Inst, Daegu 41944, South Korea; Kyungpook Natl Univ, Sch Med, Dept Biomed Sci, BK21 FOUR KNU Convergence Educ Program Biomed Sci, Daegu 41944, South Korea; Kyungpook Natl Univ, Sch Med, Dept Dermatol, Daegu 41944, South Korea; Kyungpook Natl Univ, Sch Med, Dept Radiol, Daegu 41944, South Korea; Kyungpook Natl Univ, Sch Med, Dept Surg, Daegu 41944, South Korea; Kyungpook Natl Univ, Sch Med, Dept Pediat, Daegu 41944, South Korea | ; Ryu, Jeong Yeop/GLQ-9419-2022; Lee, Jae-Hyun/ABE-3803-2020; Lee, Jongmin/AAR-6361-2020; LEE, JIN/Q-3108-2018 | 56496041000; 57211205767; 56366349400; 35746789300; 57261065200; 57735418300; 56013454400; 55689919700; 56016177400; 7101832929; 57205408095; 7404007181 | chunghoyun@gmail.com; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 23 | 16 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.35 | 2025-06-25 | 5 | 5 | arteriovenous malformation; endothelial cell; hypoxia; angiogenesis | FOLLISTATIN-LIKE 1; PROGENITOR CELLS; PERIVASCULAR CELLS; TISSUE MASS; EXPRESSION; VASCULOGENESIS; PROLIFERATION; CANCER | angiogenesis; arteriovenous malformation; endothelial cell; hypoxia | Angiogenesis Inducing Agents; Endothelial Cells; Follistatin-Related Proteins; Humans; Hypoxia; Intracranial Arteriovenous Malformations; Neovascularization, Pathologic; bone marrow stromal antigen 2; chondroitin sulfate proteoglycan 4; follistatin like 1; glyceraldehyde 3 phosphate dehydrogenase; MARCKS protein; matrigel; protein; unclassified drug; angiogenic factor; follistatin related protein; FSTL1 protein, human; angiogenesis; arteriovenous malformation; Article; clinical article; comparative study; controlled study; endothelium cell; gene expression; human; human cell; human tissue; hypoxia; immunocytochemistry; immunofluorescence; immunoreactivity; in vitro study; quantitative analysis; real time polymerase chain reaction; tubulogenesis; brain arteriovenous malformation; endothelium cell; genetics; hypoxia; metabolism; neovascularization (pathology) | English | 2022 | 2022-08 | 10.3390/ijms23169109 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Identification of a Major QTL and Validation of Related Genes for Tiller Angle in Rice Based on QTL Analysis | An ideal plant architecture is an important condition to achieve high crop yields. The tiller angle is an important and complex polygenic trait of rice (Oryza sativa L.) plant architecture. Therefore, the discovery and identification of tiller angle-related genes can aid in the improvement of crop architecture and yield. In the present study, 222 SSR markers were used to establish a high-density genetic map of rice doubled haploid population, and a total of 8 quantitative trait loci (QTLs) were detected based on the phenotypic data of the tiller angle and tiller crown width over 2 years. Among them, four QTLs (qTA9, qCW9, qTA9-1, and qCW9-1) were overlapped at marker interval RM6235-RM24288 on chromosome 9 with a large effect value regarded as a stable major QTL. The selected promising related genes were further identified by relative gene expression analysis, which gives us a basis for the future cloning of these genes. Finally, OsSAURq9, which belongs to the SMALL AUXIN UP RNA (SAUR), an auxin-responsive protein family, was selected as a target gene. Overall, this work will help broaden our knowledge of the genetic control of tiller angle and tiller crown width, and this study provides both a good theoretical basis and a new genetic resource for the breeding of ideal-type rice. | Zhao, Dan-Dan; Jang, Yoon-Hee; Farooq, Muhammad; Park, Jae-Ryoung; Kim, Eun-Gyeong; Du, Xiao-Xuan; Jan, Rahmatullah; Kim, Kyung-Hwan; Lee, Soo In; Lee, Gang-Seob; Kim, Kyung-Min | Kyungpook Natl Univ, Grad Sch, Dept Appl Biosci, Daegu 41566, South Korea; Natl Inst Crop Sci, Crop Breeding Div, Rural Dev Adm, Wonju 55365, South Korea; Natl Inst Agr Sci, Dept Agr Biotechnol, Rural Dev Adm, Jeonju 54874, South Korea; Kyungpook Natl Univ, Coastal Agr Res Inst, Daegu 41566, South Korea | ; Jan, Rahmatullah/AIC-3439-2022; Lee, Ho-jeong/S-4934-2019; Du, Xiaoxuan/AGZ-8638-2022; ZHAO, DANDAN/HIK-2130-2022; Kim, Kyung/J-5382-2012; Kim, Kyung-Min Kim/C-7007-2014 | 57459517300; 57219901992; 57215544380; 57211205505; 57221496070; 57209098077; 57201981969; 57202965738; 56648564100; 25927158200; 34868260300 | qx288mm@naver.com;uniunnie@naver.com;mfarooqsr@gmail.com;icd92@naver.com;dkqkxk632@naver.com;haobingshuaike@hotmail.com;rehmatbot@yahoo.com;biopiakim@korea.kr;silee@korea.kr;kangslee@korea.kr;kkm@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 9 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.62 | 2025-06-25 | 7 | 7 | plant architecture; rice; tiller angle; quantitative trait loci; auxin; breeding | QUANTITATIVE TRAIT LOCI; PLANT ARCHITECTURE; AUXIN SYNTHESIS; RESISTANCE; GRAVITROPISM; EXPRESSION; TRANSPORT; PROGRESS; LAZY1; TAC1 | auxin; breeding; plant architecture; quantitative trait loci; rice; tiller angle | Chromosome Mapping; Indoleacetic Acids; Oryza; Phenotype; Plant Breeding; Quantitative Trait Loci; auxin; plant protein; indoleacetic acid derivative; Article; gene expression profiling; gene mapping; genetic regulation; genetic resource; nonhuman; ossaurq9 gene; phenotype; plant chromosome; plant gene; plant structures; quantitative trait locus; rice; theoretical study; chromosomal mapping; genetics; Oryza; plant breeding | English | 2022 | 2022-05 | 10.3390/ijms23095192 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Inhibitory Activities of Rare Ginsenoside Rg4 on Cecal Ligation and Puncture-Induced Sepsis | Sepsis is an uncontrolled response to inflammatory infection and is associated with high levels of mortality and morbidity. Rg4 is a rare ginsenoside mainly found in the leaves of Panax ginseng C. A. Meyer and the major protopanaxatriol-type ginsenoside of black ginseng. In this study, we determined whether Rg4 affects cecal ligation and puncture (CLP)-induced sepsis. Animals were separated into the following six groups: control group, CLP-operated group, CLP plus maslinic acid (MA), and CLP plus Rg4 (5, 10, or 15 mg/kg). Survival rate, body weight changes, inflammatory cytokines, and histological analyses were assessed. Human endothelial cells were activated with the high-mobility group box 1 (HMGB1) protein and Rg4. Cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Enzyme-linked immunosorbent assay (ELISA) and Western blot analysis were used to assess inflammation and gene expression, respectively. After CLP surgery, the Rg4-administered group exhibited a higher survival rate and body weight compared with the untreated control group. Rg4 treatment reduced cytokine levels, including tumor necrosis factor (TNF)-alpha and interleukin (IL)-1 beta, as well as nitric oxide (NO) levels and renal inflammation. After Rg4 treatment of HMGB1-activated cells, the expressions of toll-like receptor (TLR) 4 and TNF-alpha were decreased, and the activation of phosphoinositide 3-kinase (PI3K)/AKT signaling increased cell viability. In summary, Rg4 inhibited inflammation and exhibited a protective effect against CLP-induced sepsis, thereby reinforcing cell survival against septic responses. | Kim, Go Oun; Kim, Nayeon; Song, Gyu Yong; Bae, Jong-Sup | Kyungpook Natl Univ, Coll Pharm, Res Inst Pharmaceut Sci, 80 Daehak Ro, Daegu 41566, South Korea; Chungnam Natl Univ, Coll Pharm, 99 Daehak Ro, Daejon 34134, South Korea | Bae, Jong-Sup/AAU-9724-2020 | 57896809800; 57226179942; 7402253074; 16021543200 | gysong@cnu.ac.kr;baejs@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 18 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.8 | 2025-06-25 | 9 | 9 | ginsenoside Rg4; CLP; sepsis; kidney injury; inflammation | PANAX-GINSENG; ANIMAL-MODELS; APOPTOSIS; CELLS | CLP; ginsenoside Rg4; inflammation; kidney injury; sepsis | Animals; Body Weight; Cytokines; Endothelial Cells; Ginsenosides; HMGB1 Protein; Humans; Inflammation; Ligation; Nitric Oxide; Panax; Phosphatidylinositol 3-Kinase; Phosphatidylinositol 3-Kinases; Proto-Oncogene Proteins c-akt; Punctures; Sepsis; Toll-Like Receptor 4; Tumor Necrosis Factor-alpha; 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide; eosin; ginsenoside; hematoxylin; high mobility group B1 protein; interleukin 1beta; maslinic acid; nitric oxide; phosphatidylinositol 3 kinase; toll like receptor 4; tumor necrosis factor; cytokine; ginsenoside; ginsenoside Rg4; high mobility group B1 protein; nitric oxide; phosphatidylinositol 3 kinase; protein kinase B; toll like receptor 4; tumor necrosis factor; animal cell; animal experiment; Article; body weight; body weight change; cecal ligation and puncture-induced sepsis; cell survival; cell viability; cell viability assay; controlled study; endothelium cell; enzyme linked immunosorbent assay; gene expression; ginseng; hepatitis; histology; in vitro study; male; morbidity; mortality; mouse; MTT assay; nephritis; nonhuman; Pi3K/Akt signaling; protein expression; signal transduction; survival rate; Western blotting; animal; genetics; human; inflammation; ligation; metabolism; Panax; puncture; sepsis | English | 2022 | 2022-09 | 10.3390/ijms231810836 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Long Non-Coding RNA KCNQ1OT1 Regulates Protein Kinase CK2 Via miR-760 in Senescence and Calorie Restriction | Long non-coding RNAs (lncRNAs) play important biological roles. Here, the roles of the lncRNA KCNQ1OT1 in cellular senescence and calorie restriction were determined. KCNQ1OT1 knockdown mediated various senescence markers (increased senescence-associated beta-galactosidase staining, the p53-p21(Cip1/WAF1) pathway, H3K9 trimethylation, and expression of the senescence-associated secretory phenotype) and reactive oxygen species generation via CK2 alpha downregulation in human cancer HCT116 and MCF-7 cells. Additionally, KCNQ1OT1 was downregulated during replicative senescence, and its silencing induced senescence in human lung fibroblast IMR-90 cells. Additionally, an miR-760 mimic suppressed KCNQ1OT1-mediated CK2 alpha upregulation, indicating that KCNQ1OT1 upregulated CK2 alpha by sponging miR-760. Finally, the KCNQ1OT1-miR-760 axis was involved in both lipopolysaccharide-mediated CK2 alpha reduction and calorie restriction (CR)-mediated CK2 alpha induction in these cells. Therefore, for the first time, this study demonstrates that the KCNQ1OT1-miR-760-CK2 alpha pathway plays essential roles in senescence and CR, thereby suggesting that KCNQ1OT1 is a novel therapeutic target for an alternative treatment that mimics the effects of anti-aging and CR. | Lee, Yoonsung; Bae, Young-Seuk | Kyungpook Natl Univ, Sch Life Sci, FOUR KNU Creat BioRes Grp BK21, Daegu 41566, South Korea | Bae, Youn-Sang/G-8073-2012 | 57446040000; 8230659600 | lyslys8621@naver.com;ysbae@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 3 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.44 | 2025-06-25 | 5 | 5 | KCNQ1OT1; long non-coding RNA; senescence; calorie restriction; protein kinase CK2; miR-760 | COLORECTAL-CANCER; CELLULAR SENESCENCE; DOWN-REGULATION; CKII; MIR-186; LNCRNA; LEVEL | Calorie restriction; KCNQ1OT1; Long non-coding RNA; MiR-760; Protein kinase CK2; Senescence | Caloric Restriction; Casein Kinase II; Cell Line; Cellular Senescence; Down-Regulation; Fibroblasts; Gene Knockdown Techniques; HCT116 Cells; Humans; Lipopolysaccharides; MCF-7 Cells; MicroRNAs; Neoplasms; Potassium Channels, Voltage-Gated; Reactive Oxygen Species; Senescence-Associated Secretory Phenotype; beta galactosidase; casein kinase II; casein kinase II alpha; cyclin dependent kinase inhibitor 1; interleukin 1beta; interleukin 6; long untranslated RNA; microRNA; microRNA 760; protein p21; protein p53; reactive oxygen metabolite; stromelysin; unclassified drug; casein kinase II; CSNK2A1 protein, human; KCNQ1OT1 long non-coding RNA, human; lipopolysaccharide; microRNA; MIRN760 microRNA, human; voltage gated potassium channel; acetylation; Article; caloric restriction; cell aging; cell counting; cell viability; cell viability assay; controlled study; down regulation; gene knockdown; gene silencing; genetic transfection; HCT 116 cell line; human; human cell; immunoblotting; IMR-90 cell line; MCF-7 cell line; methylation; phenotype; real time polymerase chain reaction; RNA interference; signal transduction; upregulation; adverse event; caloric restriction; cell aging; cell line; cytology; drug effect; fibroblast; genetics; metabolism; neoplasm | English | 2022 | 2022-02 | 10.3390/ijms23031888 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Mesenchymal Stem Cells and Extracellular Vesicles Derived from Canine Adipose Tissue Ameliorates Inflammation, Skin Barrier Function and Pruritus by Reducing JAK/STAT Signaling in Atopic Dermatitis | Canine atopic dermatitis (AD) is a common chronic inflammatory skin disorder resulting from imbalance between T lymphocytes. Current canine AD treatments use immunomodulatory drugs, but some of the dogs have limitations that do not respond to standard treatment, or relapse after a period of time. Thus, the purpose of this study was to evaluate the immunomodulatory effect of mesenchymal stem cells derived from canine adipose tissue (cASCs) and cASCs-derived extracellular vesicles (cASC-EVs) on AD. First, we isolated and characterized cASCs and cASCs-EVs to use for the improvement of canine atopic dermatitis. Here, we investigated the effect of cASCs or cASC-EVs on DNCB-induced AD in mice, before using for canine AD. Interestingly, we found that cASCs and cASC-EVs improved AD-like dermatitis, and markedly decreased levels of serum IgE, (49.6%, p = 0.002 and 32.1%, p = 0.016 respectively) epidermal inflammatory cytokines and chemokines, such as IL-4 (32%, p = 0.197 and 44%, p = 0.094 respectively), IL-13 (47.4%, p = 0.163, and 50.0%, p = 0.039 respectively), IL-31 (64.3%, p = 0.030 and 76.2%, p = 0.016 respectively), RANTES (66.7%, p = 0.002 and 55.6%, p = 0.007) and TARC (64%, p = 0.016 and 86%, p = 0.010 respectively). In addition, cASCs or cASC-EVs promoted skin barrier repair by restoring transepidermal water loss, enhancing stratum corneum hydration and upregulating the expression levels of epidermal differentiation proteins. Moreover, cASCs or cASC-EVs reduced IL-31/TRPA1-mediated pruritus and activation of JAK/STAT signaling pathway. Taken together, these results suggest the potential of cASCs or cASC-EVs for the treatment of chronic inflammation and damaged skin barrier in AD or canine AD. | Kim, Sung Youl; Yoon, Tae Hong; Na, Jungtae; Yi, Seong Joon; Jin, Yunseok; Kim, Minji; Oh, Tae-Ho; Chung, Tae-Wook | GNG CELL Co Ltd, R&D Ctr, 122 Unjung Ro, Seongnam Si 13466, South Korea; Sogang Univ, Dept Life Sci, 35 Baekbeom Ro, Seoul 04107, South Korea; Kyungpook Natl Univ, Coll Vet Med, Dept Vet Anat, 80 Daehak Ro, Daegu 41566, South Korea; Kyungpook Natl Univ, Coll Vet Med, Dept Vet Internal Med, 80 Daehak Ro, Daegu 41566, South Korea; Pusan Natl Univ, R&D Ctr, Natl Clin Res Ctr Korean Med, JIN BioCell Co Ltd,Korean Med Hosp, 20 Geumo Ro, Yangsan Si 50612, South Korea | 59782054800; 57653538100; 57200994259; 55918870300; 57411559400; 57127351600; 8251199600; 56803133900 | ks10204@naver.com;enteryth@naver.com;pugokjebi@gmail.com;sjyi@knu.ac.kr;jinyunseok@naver.com;kmg532@hanmail.net;thoh@knu.ac.kr;chungtw@hanmail.net; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 9 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 2.03 | 2025-06-25 | 20 | 23 | atopic dermatitis; canine; mesenchymal stem cell; extracellular vesicle; inflammation; skin barrier function; pruritus | ALLERGIC AIRWAY INFLAMMATION; CONTACT HYPERSENSITIVITY; BONE-MARROW; EFFICACY; DISEASE; BLOOD; DOGS; ACTIVATION; EXPRESSION; TGF-BETA-1 | atopic dermatitis; canine; extracellular vesicle; inflammation; mesenchymal stem cell; pruritus; skin barrier function | Adipose Tissue; Animals; Cytokines; Dermatitis, Atopic; Dogs; Extracellular Vesicles; Inflammation; Mesenchymal Stem Cells; Mice; Pruritus; Signal Transduction; Skin; 1 chloro 2,4 dinitrobenzene; alpha5 integrin; beta1 integrin; CD41b antigen; CD81 antigen; CD9 antigen; cytokeratin 1; endoglin; fibrinogen receptor; filaggrin; Hermes antigen; HLA antigen; immunoglobulin E; interleukin 13; interleukin 31; interleukin 31 receptor alpha; interleukin 4; involucrin; Janus kinase; loricrin; messenger RNA; oncostatin M receptor; RANTES; STAT protein; STAT1 protein; STAT3 protein; thymic stromal lymphopoietin; thymus and activation regulated chemokine; transient receptor potential channel A1; unclassified drug; cytokine; adipose tissue; animal cell; animal experiment; animal model; animal tissue; Article; atopic dermatitis; cell differentiation; chronic inflammation; controlled study; dog; enzyme activation; epidermal thickness; exosome; gene expression level; immunogenicity; immunoglobulin blood level; immunomodulation; JAK-STAT signaling; male; mesenchymal stem cell; mouse; nonhuman; protein blood level; pruritus; skin barrier function; skin function; skin water loss; stratum corneum; upregulation; adipose tissue; animal; atopic dermatitis; dog; inflammation; metabolism; pruritus; signal transduction; skin | English | 2022 | 2022-05 | 10.3390/ijms23094868 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | MicroRNA-365a/b-3p as a Potential Biomarker for Hypertrophic Scars | The clinical aspects of hypertrophic scarring vary according to personal constitution and body part. However, the mechanism of hypertrophic scar (HS) formation remains unclear. MicroRNAs (miRNAs) are known to contribute to HS formation, however, their detailed role remains unknown. In this study, candidate miRNAs were identified and analyzed as biomarkers of hypertrophic scarring for future clinical applications. HSfibroblasts and normal skin fibroblasts from patients were used for profiling and validation of miRNAs. An HS mouse model with xenografted human skin on nude mice was established. The miRNA expression between normal human, normal mouse, and mouse HS skin tissues was compared. Circulating miRNA expression levels in the serum of normal mice and mice with HSs were also analyzed. Ten upregulated and twenty-one downregulated miRNAs were detected. Among these, miR-365a/b-3p and miR-16-5p were identified as candidate miRNAs with statistically significant differences; miR-365a/b-3p was significantly upregulated (p = 0.0244). In mouse studies, miR-365a/b-3p expression levels in skin tissue and serum were higher in mice with HSs than in the control group. These results indicate that miRNAs contribute to hypertrophic scarring and that miR-365a/b-3p may be considered a potential biomarker for HS formation. | Lee, Joon Seok; Kim, Gyeong Hwa; Lee, Jong Ho; Ryu, Jeong Yeop; Oh, Eun Jung; Kim, Hyun Mi; Kwak, Suin; Hur, Keun; Chung, Ho Yun | Kyungpook Natl Univ, Sch Med, Dept Plast & Reconstruct Surg, Daegu 41944, South Korea; Kyungpook Natl Univ, Sch Med, Dept Biochem & Cell Biol, Daegu 41199, South Korea; Kyungpook Natl Univ, Sch Med, CMRI, Daegu 41944, South Korea; Kyungpook Natl Univ, Sch Med, Dept Biomed Sci, BK21 FOUR KNU Convergence Educ Program Biomed Sci, Daegu 41199, South Korea; Kyungpook Natl Univ, Biomed Res Inst, Daegu 41944, South Korea | ; Ryu, Jeong Yeop/GLQ-9419-2022; Hur, Keun/G-9513-2011; Kim, Gyeonghwa/AAQ-6424-2021; LEE, JIN/Q-3108-2018 | 56496041000; 57195957884; 57212661146; 56366349400; 35746789300; 57261065200; 57735418300; 8861888000; 7404007181 | leejspo@knu.ac.kr;med.aurora1106@gmail.com;clerk0823@naver.com;rjyflying@naver.com;fullrest74@hanmail.net;sarang7939@naver.com;suin8349@naver.com;keunhur@knu.ac.kr;hy-chung@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 11 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.44 | 2025-06-25 | 5 | 5 | microRNA; hypertrophic scar; myofibroblast | NUDE-MOUSE MODEL; CANCER CELLS; FIBROBLASTS; EXPRESSION; SKIN; PROGRESSION; APOPTOSIS; MIR-365 | hypertrophic scar; microRNA; myofibroblast | Animals; Biomarkers; Cicatrix, Hypertrophic; Circulating MicroRNA; Gene Expression Profiling; Humans; Mice; Mice, Nude; MicroRNAs; biological marker; circulating microRNA; microRNA; animal; gene expression profiling; genetics; human; hypertrophic scar; metabolism; mouse; nude mouse | English | 2022 | 2022-06 | 10.3390/ijms23116117 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Review | New Aspects on the Treatment of Retinopathy of Prematurity: Currently Available Therapies and Emerging Novel Therapeutics | Retinopathy of prematurity (ROP) is a rare proliferative ocular disorder in preterm infants. Because of the advancements in neonatal care, the incidence of ROP has increased gradually. Now, ROP is one of the leading causes of blindness in children. Preterm infants with immature retinal development are exposed to supplemental oxygen inside an incubator until their cardiopulmonary system is adequately developed. Once they are returned to room air, the relatively low oxygen level stimulates various angiogenesis factors initiating retinal neovascularization. If patients with ROP are not offered adequate and timely treatment, they can experience vision loss that may ultimately lead to permanent blindness. Although laser therapy and anti-vascular endothelial growth factor agents are widely used to treat ROP, they have limitations. Thus, it is important to identify novel therapeutics with minimal adverse effects for the treatment of ROP. To date, various pharmacologic and non-pharmacologic therapies have been assessed as treatments for ROP. In this review, the major molecular factors involved in the pathogenesis of ROP, currently offered therapies, therapies under investigation, and emerging novel therapeutics of ROP are discussed. | Ryu, Juhee | Kyungpook Natl Univ, Coll Pharm, Vessel Organ Interact Res Ctr, Daegu 41566, South Korea; Kyungpook Natl Univ, Pharmaceut Sci Res Inst, Coll Pharm, Daegu 41566, South Korea | 57208255566 | juheeryu@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 15 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.95 | 2025-06-25 | 28 | 28 | retinopathy of prematurity; pharmacotherapy; retinal neovascularization; preterm infant; retinal vascular disease | ENDOTHELIAL GROWTH-FACTOR; OXYGEN-INDUCED RETINOPATHY; MONOCYTE LINEAGE CELLS; RETINAL NEOVASCULARIZATION; PRETERM INFANTS; IGF-I; MOUSE MODEL; INTRAVITREAL AFLIBERCEPT; VASCULAR-PERMEABILITY; BEVACIZUMAB AVASTIN | pharmacotherapy; preterm infant; retinal neovascularization; retinal vascular disease; retinopathy of prematurity | Blindness; Child; Humans; Infant; Infant, Newborn; Infant, Premature; Infant, Premature, Diseases; Oxygen; Retinopathy of Prematurity; aflibercept; angiopoietin; antioxidant; beta adrenergic receptor blocking agent; bevacizumab; caffeine; corticosteroid; erythropoietin; hypoxia inducible factor 1; metalloproteinase; nonsteroid antiinflammatory agent; oxygen; pegaptanib; polyunsaturated fatty acid; ranibizumab; retinol; somatomedin C; untranslated RNA; vasculotropin; cell therapy; gene therapy; human; laser coagulation; laser therapy; light exposure; newborn; newborn surgery; oxygen therapy; pathogenesis; retina surgery; retrolental fibroplasia; Review; blindness; child; complication; infant; prematurity; retrolental fibroplasia | English | 2022 | 2022-08 | 10.3390/ijms23158529 | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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