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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | ○ | Article | Anti-Metastatic Effect of Pyruvate Dehydrogenase Kinase 4 Inhibition in Bladder Cancer via the ERK, SRC, and JNK Pathways | Bladder cancer is a common global cancer with a high percentage of metastases and high mortality rate. Thus, it is necessary to identify new biomarkers that can be helpful in diagnosis. Pyruvate dehydrogenase kinase 4 (PDK4) belongs to the PDK family and plays an important role in glucose utilization in living organisms. In the present study, we evaluated the role of PDK4 in bladder cancer and its related protein changes. First, we observed elevated PDK4 expression in high-grade bladder cancers. To screen for changes in PDK4-related proteins in bladder cancer, we performed a comparative proteomic analysis using PDK4 knockdown cells. In bladder cancer cell lines, PDK4 silencing resulted in a lower rate of cell migration and invasion. In addition, a PDK4 knockdown xenograft model showed reduced bladder cancer growth in nude mice. Based on our results, PDK4 plays a critical role in the metastasis and growth of bladder cancer cells through changes in ERK, SRC, and JNK. | Lee, Eun Hye; Chung, Jae-Wook; Sung, Eunji; Yoon, Bo Hyun; Jeon, Minji; Park, Song; Chun, So Young; Lee, Jun Nyung; Kim, Bum Soo; Kim, Hyun Tae; Kim, Tae Hwan; Choi, Seock Hwan; Yoo, Eun Sang; Kwon, Tae Gyun; Kang, Ho Won; Kim, Wun-Jae; Yun, Seok Joong; Lee, Sangkyu; Ha, Yun-Sok | Kyungpook Natl Univ, Joint Inst Regenerat Med, Daegu 41566, South Korea; Kyungpook Natl Univ, Sch Med, Dept Urol, Daegu 41405, South Korea; Kyungpook Natl Univ, Pharmaceut Sci Res Inst, Coll Pharm, Four Community Based Intelligent Novel Drug Disco, Daegu 41566, South Korea; Daegu Gyeongbuk Inst Sci & Technol, Div Biotechnol, Daegu 42988, South Korea; Kyungpook Natl Univ Hosp, Biomed Res Inst, Daegu 41944, South Korea; Chungbuk Natl Univ, Dept Urol, Coll Med, Cheongju 28644, South Korea; Chungbuk Natl Univ Hosp, Dept Urol, Cheongju 28644, South Korea; Inst Urotech, Cheongju 28120, South Korea | Jeon, Minji/HTN-4703-2023; Kim, Soo-Yeon/ADR-9663-2022; Kim, Tae-Hwan/M-3962-2017; Kim, Jae-hyung/J-8504-2012 | 57189661699; 35204798500; 56988948200; 57218931150; 59026085100; 57139047900; 8688166900; 16301364600; 57202817150; 55739531300; 57797823600; 9742645500; 7006609239; 15073765400; 35757703900; 8081691400; 16302421300; 57209046767; 35487226400 | sangkyu@knu.ac.kr;yunsokha@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 21 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.8 | 2025-06-25 | 9 | 9 | bladder cancer; metastasis; invasion; muscle-invasive bladder cancer (MIBC); pyruvate dehydrogenase kinase 4 (PDK4) | PROTEOMICS ANALYSIS; OVARIAN-CANCER; ACTIVATION; CELLS; APOPTOSIS; PDK4; PHOSPHORYLATION; CHEMOTHERAPY; PROGNOSIS; AXIS | bladder cancer; invasion; metastasis; muscle-invasive bladder cancer (MIBC); pyruvate dehydrogenase kinase 4 (PDK4) | Animals; Humans; MAP Kinase Signaling System; Mice; Mice, Nude; Protein Serine-Threonine Kinases; Proteomics; Pyruvate Dehydrogenase Acetyl-Transferring Kinase; Urinary Bladder Neoplasms; mitogen activated protein kinase 1; protein kinase p60; pyruvate dehydrogenase kinase 4; stress activated protein kinase; pyruvate dehydrogenase kinase 4; animal cell; animal experiment; animal model; antineoplastic activity; Article; bladder cancer; bladder cancer cell line; cancer cell; cancer growth; cancer staging; cell function; cell lysate; cell metabolism; cell migration; cell migration rate; cell proliferation; citric acid cycle; controlled study; immunocompetent cell; in vitro study; J82 cell line; male; metastasis inhibition; mitochondrial respiration; mortality rate; mouse; muscle invasive bladder cancer; nonhuman; pathway enrichment analysis; protein expression; protein fingerprinting; protein function; signal transduction; survival rate; T24 cell line; tumor growth; tumor xenograft; animal; bladder tumor; genetics; human; MAPK signaling; nude mouse; proteomics | English | 2022 | 2022-11 | 10.3390/ijms232113240 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Anti-Septic Functions of Cornuside against HMGB1-Mediated Severe Inflammatory Responses | High mobility group box 1 (HMGB1) is acknowledged to have critical functions; therefore, targeting this protein may have therapeutic effects. One example is potential antiseptic activity obtained by suppressing HMGB1 secretion, leading to the recovery of vascular barrier integrity. Cornuside (CN), which is a product extracted from the fruit of Cornus officinalis Seib, is a natural bis-iridoid glycoside with the therapeutic effects of suppressing inflammation and regulating immune responses. However, the mechanism of action of CN and impact on sepsis is still unclear. We examined if CN could suppress HMGB1-induced excessive permeability and if the reduction of HMGB1 in response to LPS treatment increased the survival rate in a mouse model of sepsis. In human endothelial cells stimulated by LPS and mice with septic symptoms of cecal ligation and puncture (CLP), we examined levels of proinflammatory proteins and biomarkers as an index of tissue damage, along with decreased vascular permeability. In both LPS-treated human umbilical vein endothelial cells (HUVECs) and the CLP-treated mouse model of sepsis, we applied CN after the induction processes were over. CN suppressed excessive permeability and inhibited HMGB1 release, leading to the amelioration of vascular instability, reduced mortality, and improved histological conditions in the CLP-induced septic mouse model. Overall, we conclude that the suppressed release of HMGB1 and the increased survival rate of mice with CLP-induced sepsis caused by CN may be an effective pharmaceutical treatment for sepsis. | Kim, Nayeon; Kim, Chaeyeong; Ryu, Soo Ho; Lee, Wonhwa; Bae, Jong-Sup | Kyungpook Natl Univ, Pharmaceut Sci Res Inst, Coll Pharm, 80 Daehak Ro, Daegu 41566, South Korea; Sungkyunkwan Univ, Dept Chem, 2066 Seobu Ro, Suwon 16419, South Korea | Bae, Jong-Sup/AAU-9724-2020; Lee, Wonhwa/GLQ-6506-2022 | 57226179942; 57418696700; 57418127000; 50161632800; 16021543200 | cgullinny@naver.com;rlacodud1213@naver.com;rsh604@gmail.com;Wonhwalee@skku.edu;baejs@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 4 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 1.06 | 2025-06-25 | 12 | 12 | cornuside; HMGB1; endothelium; sepsis | NF-KAPPA-B; GROUP BOX 1; IMPROVES SURVIVAL; PROTEIN HMGB1; LIPOPOLYSACCHARIDE; ZINGERONE; CELLS; MAPK; PHOSPHORYLATION; ACTIVATION | Cornuside; Endothelium; HMGB1; Sepsis | Animals; Cell Adhesion; Cell Movement; Cells, Cultured; Cytokines; Disease Models, Animal; Glucosides; HMGB1 Protein; Human Umbilical Vein Endothelial Cells; Humans; Inflammation; Male; Mice; Mice, Inbred C57BL; Pyrans; Sepsis; Signal Transduction; alanine aminotransferase; albumin; aspartate aminotransferase; biological marker; cornuside; creatinine; cyclooxygenase 2; gelatinase B; glycoside; high mobility group B1 protein; immunoglobulin enhancer binding protein; intercellular adhesion molecule 1; interleukin 1beta; interleukin 2; interleukin 6; lactate dehydrogenase; lamin B; mitogen activated protein kinase; mitogen activated protein kinase p38; monocyte chemotactic protein 1; prostaglandin E2; protein p53; sirtuin 1; stress activated protein kinase; toll like receptor 2; toll like receptor 4; transcription factor Nrf2; tumor necrosis factor; unclassified drug; vascular cell adhesion molecule 1; zingerone; cornuside; cytokine; glucoside; high mobility group B1 protein; HMGB1 protein, human; pyran derivative; animal cell; animal experiment; animal model; animal tissue; antimicrobial activity; Article; blood vessel permeability; cecal ligation and puncture-induced sepsis; cell adhesion assay; cell invasion assay; cell migration; cell viability; chromosome translocation; controlled study; Cornus officinalis; deacetylation; down regulation; endothelium; endothelium cell; enzyme linked immunosorbent assay; fluorescence microscopy; histology; histopathology; human; human cell; HUVEC cell line; hypoxemia; immunoprecipitation; inflammation; kidney injury; leukocyte; male; mouse; MTT assay; nonhuman; oxidative stress; protein acetylation; protein expression; protein phosphorylation; real time polymerase chain reaction; signal transduction; survival rate; therapy effect; tissue injury; urea nitrogen blood level; Western blotting; animal; C57BL mouse; cell adhesion; cell culture; cell motion; disease model; drug effect; metabolism; sepsis; umbilical vein endothelial cell | English | 2022 | 2022-02 | 10.3390/ijms23042065 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Anti-Thrombotic Effects of Artesunate through Regulation of cAMP and PI3K/MAPK Pathway on Human Platelets | Normal activation of platelets and their aggregation are crucial for proper hemostasis. It appears that excessive or abnormal aggregation of platelets may bring about cardiovascular diseases such as stroke, atherosclerosis, and thrombosis. For this reason, finding a substance that can regulate platelet aggregation or suppress aggregation will aid in the prevention and treatment of cardiovascular diseases. Artesunate is a compound extracted from the plant roots of Artemisia or Scopolia, and its effects have shown to be promising in areas of anticancer and Alzheimer's disease. However, the role and mechanisms by which artesunate affects the aggregation of platelets and the formation of a thrombus are currently not understood. This study examines the ways artesunate affects the aggregation of platelets and the formation of a thrombus on platelets induced by U46619. As a result, cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) production were increased significantly by artesunate relative to the doses, as well as phosphorylated vasodilator-stimulated phosphoprotein (VASP) and inositol 1,4,5-trisphosphate receptor (IP3R), substrates to cAMP-dependent kinase and cGMP-dependent kinase, in a significant manner. The Ca2+, normally mobilized from the dense tubular system, was inhibited due to IP3R phosphorylation from artesunate, and phosphorylated VASP aided in inhibiting platelet activity via alpha IIb/beta(3) platelet membrane inactivation and inhibiting fibrinogen binding. In addition, MAPK and PI3K/Akt phosphorylation was inhibited via artesunate in a significant manner, causing the production of TXA(2) and intracellular granular secretion (serotonin and ATP release) to be reduced. Therefore, we suggest that artesunate has value as a substance that inhibits platelet aggregation and thrombus formation through an antiplatelet mechanism. | Yoon, Shin-Sook; Kwon, Hyuk-Woo; Shin, Jung-Hae; Rhee, Man Hee; Park, Chang-Eun; Lee, Dong-Ha | Namseoul Univ, Dept Biomed Lab Sci, Cheonan 31020, South Korea; Far East Univ, Dept Biomed Lab Sci, Eumseong 27601, South Korea; Kyungpook Natl Univ, Dept Vet Med, Coll Vet Med, Daegu 41566, South Korea; Namseoul Univ, Mol Diagnost Res Inst, Cheonan 31020, South Korea | Park, Chang-Eun/AEW-4266-2022; Rhee, Man/O-5705-2016 | 57428370900; 55200547400; 56244056800; 57211035357; 57190954987; 57208891222 | yss28@hanmail.net;hyuk-woo@hanmail.net;mlsjshin@naver.com;rheemh@knu.ac.kr;pce@nsu.ac.kr;dhlee@nsu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 3 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 1.94 | 2025-06-25 | 18 | 22 | artesunate; platelet aggregation; cyclic nucleotide; intracellular Ca2+; granule secretion | STIMULATED PHOSPHOPROTEIN VASP; CYTOSOLIC PHOSPHOLIPASE A(2); ACTIVATED PROTEIN-KINASES; INDUCED LUNG INJURY; THROMBUS FORMATION; PHOSPHORYLATION; RECEPTOR; PHOSPHODIESTERASES; AGGREGATION; INHIBITION | Artesunate; Cyclic nucleotide; Granule secretion; Intracellular Ca<sup>2+</sup>; Platelet aggregation | 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; Artesunate; Calcium; Cyclic AMP; Cyclic GMP; Fibrinolytic Agents; Gene Expression Regulation; Humans; MAP Kinase Signaling System; Phosphatidylinositol 3-Kinases; Phosphorylation; Platelet Aggregation; Thromboxane A2; arachidonic acid; artesunate; collagen; cyclic AMP; cyclic GMP; dimethyl sulfoxide; fibrinogen; inositol 1,4,5 trisphosphate receptor; serotonin; stress activated protein kinase; synaptophysin; thrombin; thrombocyte antigen; thromboxane A2; ticagrelor; vasodilator stimulated phosphoprotein; 15 hydroxy 11alpha,9alpha epoxymethanoprosta 5,13 dienoic acid; artesunate; calcium; cyclic AMP; cyclic GMP; fibrinolytic agent; phosphatidylinositol 3 kinase; thromboxane A2; Akt signaling; Alzheimer disease; antithrombotic activity; Artemisia; Article; atherosclerosis; blood clotting; calcium cell level; cardiovascular disease; cerebrovascular accident; fibrin clot; hemostasis; human; IC50; MAPK signaling; protein phosphorylation; Scopolia; signal transduction; thrombocyte; thrombocyte activation; thrombocyte aggregation; thrombosis; drug effect; gene expression regulation; MAPK signaling; metabolism; phosphorylation | English | 2022 | 2022-02 | 10.3390/ijms23031586 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Association between physical activity and risk of renal function decline and mortality in community-dwelling older adults: a nationwide population-based cohort study | Background: Physical activity (PA) is an important risk factor associated with health outcomes. However, the relationship between PA and kidney function decline in older adults remains unclear. We examined the influence of PA on kidney function decline and mortality in community-dwelling older adults.Methods: Adults aged & GE; 65 years with an estimated glomerular filtration rate (eGFR) > 60 mL/min/1.73 m(2) who had available health checkup data from 2009 to 2010 were included. The cohort was followed annually through December 2015 for anthropometric, sociodemographic, and medical information including outcomes and biennially for laboratory information from the health checkup. We divided these patients into three groups according to self-reported PA (Inactive group: no leisure-time PA, Active group: vigorous activity for at least 80 min/week or a sum of moderate-intensity activity and walking for at least 300 min/week, Low-active group: level of PA between the definitions of the other two groups). Associations between the intensity of PA and death, cardiovascular death, and & GE; 50% eGFR decline were investigated.Results: Among 102,353 subjects, 32,984 (32.23%), 54,267 (53.02%), and 15,102 (14.75%) were classified into the inactive, low-active, and active groups, respectively. The active group was younger, contained a higher proportion of men, and had higher frequencies of hypertension, diabetes mellitus, drinking, and smoking than the other groups. The active group had significantly lower incidence rates of mortality, cardiovascular mortality, and kidney function decline than the other groups (all p < 0.001). The active group also showed lower all-cause (hazard ratio [HR], 0.76; 95% confidence interval [CI], 0.70-0.82) and cardiovascular mortality (HR, 0.64; 95% CI, 0.53-0.78) and protection against & GE; 50% eGFR decline (HR, 0.81; 95% CI, 0.68-0.97) compared with the inactive group in the fully adjusted Cox proportional hazards regression model.Conclusions: High PA was an independent modifiable lifestyle factor for reducing mortality and protecting against declines in kidney function in older adults. | Kim, Hyunsuk; Ko, Mun Jung; Lim, Chi-Yeon; Bae, Eunjin; Hyun, Young Youl; Chung, Sungjin; Kwon, Soon Hyo; Cho, Jang-Hee; Yoo, Kyung Don; Park, Woo Yeong; Sun, In O.; Yu, Byung Chul; Ko, Gang-Jee; Yang, Jae Won; Hwang, Won Min; Song, Sang Heon; Shin, Sung Joon; Hong, Yu Ah | Hallym Univ, Chuncheon Sacred Heart Hosp, Dept Internal Med, Div Nephrol,Med Ctr, Chunchon, South Korea; Dongguk Univ, Dept Biostat, Coll Med, Goyang Si, South Korea; Gyeongsang Natl Univ, Dept Internal Med, Div Nephrol, Coll Med, Jinju, South Korea; Sungkyunkwan Univ, Kangbuk Samsung Hosp, Sch Med, Dept Internal Med,Div Nephrol, Seoul, South Korea; Catholic Univ Korea, Coll Med, Dept Internal Med, Div Nephrol,Yeouido St Marys Hosp, Seoul, South Korea; Soonchunhyang Univ, Dept Internal Med, Div Nephrol, Seoul Hosp, Seoul, South Korea; Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Dept Internal Med,Div Nephrol, Daegu, South Korea; Univ Ulsan, Ulsan Univ Hosp, Dept Internal Med, Div Nephrol,Coll Med, Ulsan, South Korea; Keimyung Univ, Sch Med, Dept Internal Med, Div Nephrol,Dongsan Hosp, Daegu, South Korea; Presbyterian Med Ctr, Dept Internal Med, Div Nephrol, Jeonju, South Korea; Soonchunhyang Univ, Dept Internal Med, Div Nephrol, Bucheon Hosp, Bucheon, South Korea; Korea Univ, Coll Med, Dept Internal Med, Div Nephrol,Guro Hosp, Seoul, South Korea; Yonsei Univ, Dept Internal Med, Div Nephrol, Wonju Coll Med, Wonju, South Korea; Konyang Univ Hosp, Dept Internal Med, Div Nephrol, Daejeon, South Korea; Pusan Natl Univ Hosp, Dept Internal Med, Div Nephrol, Busan, South Korea; Pusan Natl Univ Hosp, Biomed Res Inst, Busan, South Korea; Dongguk Univ, Sch Med, Dept Internal Med, Div Nephrol,Ilsan Hosp, Goyang, South Korea; Catholic Univ Korea, Coll Med, Daejeon St Marys Hosp, Div Nephrol,Dept Internal Med, 64 Daeheung Ro, Daejeon 34943, South Korea | Chung, Sungjin/AAJ-8836-2020; Yoo, Kyung/AAK-8096-2020; Cho, Jang-hee/ABD-3534-2020; Hong, Yu/AAB-7055-2020; Park, Woo Yeong/AGK-9140-2022 | 57194217507; 57644884100; 39261849000; 55880508000; 57037163300; 23388171000; 57204097241; 7403536291; 56603636300; 36344980100; 36994821600; 57203908496; 8310760600; 55038270300; 55568773500; 36162581500; 55662651000; 55125210000 | amorfati@catholic.ac.kr; | BMC GERIATRICS | BMC GERIATR | 1471-2318 | 22 | 1 | SCIE;SSCI | GERIATRICS & GERONTOLOGY;GERONTOLOGY | 2022 | 4.1 | 23.0 | 0.59 | 2025-06-25 | 5 | 5 | Physical activity; Renal function; Mortality; Older adults | CHRONIC KIDNEY-DISEASE; ALL-CAUSE MORTALITY; PREVALENCE; SARCOPENIA; EXERCISE; FRAILTY; GUIDELINES; GFR | Mortality; Older adults; Physical activity; Renal function | Aged; Cardiovascular Diseases; Cohort Studies; Exercise; Humans; Independent Living; Kidney; Male; Risk Factors; aged; cardiovascular disease; cohort analysis; exercise; human; independent living; kidney; male; physiology; risk factor | English | 2022 | 2022-12-17 | 10.1186/s12877-022-03693-1 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Benzoylpaeoniflorin Activates Anti-Inflammatory Mechanisms to Mitigate Sepsis in Cell-Culture and Mouse Sepsis Models | Xuebijing injection (XBJI) (comprising of five herbs) is a widely used traditional Chinese medicine for sepsis treatment. However, the bioactive components of XBJI and the mechanisms responsible for its sepsis-mitigating action have not been experimentally determined. One of the main bioactive compounds in XBJI-benzoylpaeoniflorin (BPF)-inhibits the expressions of key mediators of inflammation such as nuclear factor kappa B (NF-kappa B), cyclooxygenase-1 (COX-1), and COX-2. However, its effects on sepsis have not been determined yet. Therefore, here, we investigated the immunomodulatory effect of BPF on severely inflamed endothelial cells, THP-1 macrophages, peritoneal macrophages, and mice. Human umbilical vein endothelial cells (HUVECs) and THP-1-macrophages were activated using lipopolysaccharide (LPS) after pretreatment with BPF. Subsequently, changes in the expression profiles of pro-inflammatory molecules including inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-alpha, and interleukin (IL)-6 were determined using quantitative real-time polymerase chain reaction (qPCR) and Western blot analysis. Furthermore, we monitored the phosphorylation of NF-kB and mitogen-activated protein kinases (MAPKs) to determine their activation levels. Using the LPS-induced mouse model of sepsis, we studied the effects of BPF on inflammatory cytokine production, pulmonary histopathology, and survival rates. Finally, we evaluated whether BPF protects against cecal ligation and puncture (CLP)-induced sepsis, as it closely mimics human sepsis. BPF pretreatment inhibited LPS-induced increase in mRNA and protein levels of iNOS, TNF-alpha, and IL-6 in HUVECs and THP-1-macrophages. It also suppressed LPS-mediated phosphorylation of p65, p38, JNK, and ERK. Mice with LPS-induced-sepsis who were treated with BPF had lower serum levels of IL-6, TNF-alpha, IL-1 beta, CXCL1, and CXCL2 than the control mice treated with BPF. Histopathology revealed that BPF treatment alleviated LPS-induced lung damage. In addition, in mice given a lethal dose of LPS, BPF treatment showed a dose-dependent improvement in survival rates. BPF treatment dose-dependently inhibited the LPS-induced IL-6, TNF-alpha, and CXCL1 production in peritoneal macrophages. BPF treatment also dose-dependently improved the survival rates in mice with CLP-induced sepsis. These results show that BPF alleviates LPS-stimulated septic conditions and protects mice from CLP-induced sepsis. Our research marks BPF as a potential drug in the treatment of sepsis and various inflammatory diseases. | Kim, Chaeyeong; Sim, Hyunchae; Bae, Jong-Sup | Kyungpook Natl Univ, Pharmaceut Sci Res Inst, Coll Pharm, Daegu 41566, South Korea | Bae, Jong-Sup/AAU-9724-2020 | 57418696700; 57219098739; 16021543200 | baejs@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 21 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 1.24 | 2025-06-25 | 13 | 15 | benzoylpaeoniflorin; lipopolysaccharide; inflammation; endothelium; CLP; sepsis | XUEBIJING INJECTION; PROTEIN-C | benzoylpaeoniflorin; CLP; endothelium; inflammation; lipopolysaccharide; sepsis | Animals; Anti-Inflammatory Agents; Disease Models, Animal; Endothelial Cells; Humans; Interleukin-6; Lipopolysaccharides; Mice; NF-kappa B; Nitric Oxide; Nitric Oxide Synthase Type II; Sepsis; Tumor Necrosis Factor-alpha; antiinflammatory agent; benzoylpaeoniflorin; CXCL1 chemokine; CXCL2 chemokine; gamma interferon; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; interleukin 1beta; interleukin 6; lipopolysaccharide; messenger RNA; mitogen activated protein kinase; mitogen activated protein kinase p38; nitric oxide; paeoniflorin; synaptotagmin I; transcription factor AP 1; tumor necrosis factor; unclassified drug; antiinflammatory agent; benzoylpaeoniflorin; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; interleukin 6; lipopolysaccharide; tumor necrosis factor; animal experiment; animal model; antiinflammatory activity; Article; cecal ligation and puncture-induced sepsis; cell stimulation; controlled study; cytokine production; histopathology; human; human cell; immunomodulation; inflammation; lipopolysaccharide-induced sepsis; lung injury; mouse; MTT assay; nonhuman; peritoneum macrophage; real time polymerase chain reaction; sepsis; septic shock; survival; survival rate; THP-1 cell line; umbilical vein endothelial cell; Western blotting; animal; disease model; endothelium cell; metabolism; sepsis | English | 2022 | 2022-11 | 10.3390/ijms232113130 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Bio-Efficacy of Chrysoeriol7, a Natural Chemical and Repellent, against Brown Planthopper in Rice | Brown planthopper (BPH, Nilaparvata lugens Stal.) is the most damaging rice pest affecting stable rice yields worldwide. Currently, methods for controlling BPH include breeding a BPH-resistant cultivar and using synthetic pesticides. Nevertheless, the continuous cultivation of resistant cultivars allows for the emergence of various resistant races, and the use of synthetic pesticides can induce environmental pollution as well as the emergence of unpredictable new pest species. As plants cannot migrate to other locations on their own to combat various stresses, the production of secondary metabolites allows plants to protect themselves from stress and tolerate their reproduction. Pesticides using natural products are currently being developed to prevent environmental pollution and ecosystem disturbance caused by synthetic pesticides. In this study, after BPH infection in rice, chrysoeriol7 (C7), a secondary metabolite that induces resistance against BPH, was assessed. After C7 treatment and BPH infection, relative expression levels of the flavonoid-related genes were elevated, suggesting that in plants subjected to BPH, compounds related to flavonoids, among the secondary metabolites, play an important role in inducing resistance. The plant-derived natural compound chrysoeriol7 can potentially thus be used to develop environmentally friendly pesticides. The suggested control of BPH can be effectively used to alleviate concerns regarding environmental pollution and to construct a relatively safe rice breeding environment. | Kim, Eun-Gyeong; Yun, Sopheap; Park, Jae-Ryoung; Jang, Yoon-Hee; Farooq, Muhammad; Yun, Byoung-Ju; Kim, Kyung-Min | Kyungpook Natl Univ, Sch Appl Biosci, Div Plant Biosci, Coll Agr & Life Sci, Daegu 41566, South Korea; Royal Univ Phnom Penh, Grad Sch Sci, Sangkat Teuk Laak 1,Russian Federat Blvd, Toul Kork 12101, Phnom Penh, Cambodia; Rural Dev Adm, Natl Inst Crop Sci, Dept Crop Breeding, Wonju 55365, South Korea; Kyungpook Natl Univ, Sch Elect Engn, Coll IT Engn, 80 Daehak Ro, Daegu 41566, South Korea | ; Kim, Kyung-Min Kim/C-7007-2014 | 57221496070; 57190670675; 57211205505; 57219901992; 57215544380; 7006416932; 34868260300 | dkqkxk632@naver.com;yun.sopheap@rupp.edu.kh;icd92@naver.com;uniunnie@naver.com;mfarooqsr@gmail.com;bjisyun@knu.ac.kr;kkm@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 3 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.62 | 2025-06-25 | 6 | 7 | brown planthopper (Nilaparvata lugens Stal; ); rice; secondary metabolite; chrysoeriol7; environmentally friendly pesticides | RESISTANCE; FLAVONOIDS; VOLATILES; IDENTIFICATION; METABOLITES; MECHANISMS; PESTICIDES; GLYCOSIDES; DEFENSE; TRICIN | Brown planthopper (Nilaparvata lugens Stal.); Chrysoeriol7; Environmentally friendly pesticides; Rice; Secondary metabolite | Animals; Biosynthetic Pathways; Disease Resistance; Flavones; Gene Expression Regulation, Plant; Green Chemistry Technology; Hemiptera; Insect Repellents; Oryza; Plant Proteins; Secondary Metabolism; alpha tocopherol; chrysoeriol 7; flavanone; insect repellent; natural product; unclassified drug; chrysoeriol; flavone derivative; insect repellent; plant protein; Article; biotic stress; chlorophyll content; controlled study; cultivar; drug efficacy; ecosystem; female; flavonoid related gene; gene expression; high performance liquid chromatography; male; metabolite; nagdong cultivar; Nilaparvata lugens; nonhuman; physiological stress; plant gene; plant height; plant intoxication; planthopper; pollution; Pseudomonas syringae; reverse transcription polymerase chain reaction; rice; samgang cultivar; thin layer chromatography; animal; biosynthesis; chemistry; disease resistance; drug effect; gene expression regulation; genetics; green chemistry; growth, development and aging; Hemiptera; isolation and purification; Oryza; parasitology; secondary metabolism | English | 2022 | 2022-02 | 10.3390/ijms23031540 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Bioinformatics of Differentially Expressed Genes in Phorbol 12-Myristate 13-Acetate-Induced Megakaryocytic Differentiation of K562 Cells by Microarray Analysis | Megakaryocytes are large hematopoietic cells present in the bone marrow cavity, comprising less than 0.1% of all bone marrow cells. Despite their small number, megakaryocytes play important roles in blood coagulation, inflammatory responses, and platelet production. However, little is known about changes in gene expression during megakaryocyte maturation. Here we identified the genes whose expression was changed during K562 leukemia cell differentiation into megakaryocytes using an Affymetrix GeneChip microarray to determine the multifunctionality of megakaryocytes. K562 cells were differentiated into mature megakaryocytes by treatment for 7 days with phorbol 12-myristate 13-acetate, and a microarray was performed using RNA obtained from both types of cells. The expression of 44,629 genes was compared between K562 cells and mature megakaryocytes, and 954 differentially expressed genes (DEGs) were selected based on a p-value 2. The DEGs was further functionally classified using five major megakaryocyte function-associated clusters-inflammatory response, angiogenesis, cell migration, extracellular matrix, and secretion. Furthermore, interaction analysis based on the STRING database was used to generate interactions between the proteins translated from the DEGs. This study provides information on the bioinformatics of the DEGs in mature megakaryocytes after K562 cell differentiation. | Lee, Seung-Hoon; Park, Na Rae; Kim, Jung-Eun | Kyungpook Natl Univ, Sch Med, Dept Mol Med, Daegu 41944, South Korea; Kyungpook Natl Univ, Dept Biomed Sci, BK21 Four KNU Convergence Educ Program Biomed Sci, Daegu 41944, South Korea; Kyungpook Natl Univ, Cell & Matrix Res Inst, Daegu 41944, South Korea | 59056027600; 24492053700; 57209054588 | jsat1234@naver.com;nrpark85@naver.com;kjeun@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 8 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.09 | 2025-06-25 | 1 | 1 | megakaryocytes; microarray data; differentially expressed genes; STRING | ENDOTHELIAL GROWTH-FACTOR; EXTRACELLULAR-MATRIX; BONE-FORMATION; ANGIOGENESIS; TARGET; IDENTIFICATION; FIBRONECTIN; INHIBITOR; PLATELETS; REVEALS | differentially expressed genes; megakaryocytes; microarray data; STRING | Acetates; Cell Differentiation; Computational Biology; Humans; K562 Cells; Megakaryocytes; Microarray Analysis; Myristic Acid; Phorbols; Tetradecanoylphorbol Acetate; Thrombopoiesis; CD34 antigen; phorbol 13 acetate 12 myristate; RNA; transcription factor GATA 1; transcription factor GATA 2; transcription factor NF E2; acetic acid; myristic acid; phorbol; phorbol 13 acetate 12 myristate; phorbol derivative; Article; bioinformatics; cell death; cell differentiation; cell migration; comparative study; controlled study; differential gene expression; down regulation; erythroid precursor cell; extracellular matrix; functional enrichment analysis; gene expression; human; human cell; inflammation; K-562 cell line; megakaryocyte; microarray analysis; nervous system development; polyploidy; real time polymerase chain reaction; upregulation; biology; cell differentiation; K-562 cell line; megakaryocyte; metabolism; microarray analysis; thrombocytopoiesis | English | 2022 | 2022-04 | 10.3390/ijms23084221 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Cassiaside C Inhibits M1 Polarization of Macrophages by Downregulating Glycolysis | Classically activated M1 macrophages reprogram their metabolism towards enhanced glycolysis to obtain energy and produce pro-inflammatory cytokines after activation by mammalian target of rapamycin complex 1 (mTORC1) and hypoxia-inducible factor (HIF)-1 alpha. Thus, a strategy that constrains M1 polarization of macrophages via downregulation of glycolysis is essential for treating chronic inflammatory diseases. Cassiae semen has pharmacological activity against various inflammatory diseases. However, it is unclear whether specific compounds within Cassia seeds affect M1 polarization of macrophages. Here, we investigated whether Cassiaside C napthopyrone from Cassiae semen inhibits M1 polarization by downregulating glycolysis. We found that Cassiaside C reduced expression of inducible nitric oxide synthase and cyclooxygenase-2 and the phosphorylation of nuclear factor kappa B, all of which are upregulated in lipopolysaccharide (LPS)/interferon (IFN)-gamma-treated Raw264.7 cells and peritoneal macrophages. Moreover, Cassiaside C-treated macrophages showed marked suppression of LPS/IFN-gamma-induced HIF-1 alpha, pyruvate dehydrogenase kinase 1, and lactate dehydrogenase A expression, along with downregulation of the phosphoinositide 3-kinases (PI3K)/AKT/mTORC1 signaling pathway. Consequently, Cassiaside C attenuated enhanced glycolysis and lactate production, but rescued diminished oxidative phosphorylation, in M1 polarized macrophages. Thus, Cassiaside C dampens M1 polarization of macrophages by downregulating glycolysis, which could be exploited as a therapeutic strategy for chronic inflammatory conditions. | Kim, Ye Jin; Lee, Sungwoo; Jin, Jonghwa; Woo, Hyein; Choi, Yeon-Kyung; Park, Keun-Gyu | Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Dept Internal Med, Daegu 41944, South Korea; Kyungpook Natl Univ, Res Inst Aging & Metab, Daegu 41566, South Korea; Daegu Gyeongbuk Med Innovat Fdn, New Drug Dev Ctr, Daegu 41061, South Korea; Kyungpook Natl Univ, Chilgok Hosp, Sch Med, Dept Internal Med, Daegu 41404, South Korea | 57207443325; 57189250997; 57223246243; 57214147876; 35335932600; 57202558343 | freewilly59@hanmail.net;swlee@dgmif.re.kr;wooing86@gmail.com;ykchoi@knu.ac.kr;kpark@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 3 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 1.5 | 2025-06-25 | 19 | 18 | M1 polarization; macrophage; glycolysis; Cassiaside C | KAPPA-B; GLUCOSE-METABOLISM; MAMMALIAN TARGET; DENDRITIC CELLS; MTOR; CANCER; AKT; SUPPRESSION; ACTIVATION; INDUCTION | Cassiaside C; Glycolysis; M1 polarization; Macrophage | Animals; Gene Expression Regulation; Glycolysis; Macrophage Activation; Macrophages; Mechanistic Target of Rapamycin Complex 1; Mice; Mice, Inbred C57BL; Nitric Oxide Synthase Type II; Proto-Oncogene Proteins c-akt; RAW 264.7 Cells; Signal Transduction; carbohydrate; carbonyl cyanide chlorophenylhydrazone; cassiaside; gamma interferon; glycoside; interleukin 1beta; interleukin 6; lactic acid; lipopolysaccharide; nitric oxide synthase; penicillin derivative; phosphate buffered saline; phosphatidylinositide; streptomycin; thioglycolic acid; unclassified drug; inducible nitric oxide synthase; mammalian target of rapamycin complex 1; protein kinase B; animal cell; animal experiment; animal model; Article; cell culture; cell density; cell viability; centrifugation; controlled study; cytokine release; down regulation; fetal bovine serum; gene expression; glycolysis; M1 macrophage; macrophage; mouse; nonhuman; oxidative phosphorylation; phenotype; polarization; polyacrylamide gel electrophoresis; protein expression; protein phosphorylation; real time polymerase chain reaction; RNA extraction; sequence analysis; signal transduction; Western blotting; animal; C57BL mouse; drug effect; gene expression regulation; genetics; macrophage activation; metabolism; RAW 264.7 cell line; signal transduction | English | 2022 | 2022-02 | 10.3390/ijms23031696 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Characterization of the RAS/RAF/ERK Signal Cascade as a Novel Regulating Factor in Alpha-Amanitin-Induced Cytotoxicity in Huh-7 Cells | The well-known hepatotoxicity mechanism resulting from alpha-amanitin (alpha-AMA) exposure arises from RNA polymerase II (RNAP II) inhibition. RNAP II inhibition occurs through the dysregulation of mRNA synthesis. However, the signaling pathways in hepatocytes that arise from alpha-AMA have not yet been fully elucidated. Here, we identified that the RAS/RAF/ERK signaling pathway was activated through quantitative phosphoproteomic and molecular biological analyses in Huh-7 cells. Bioinformatics analysis showed that alpha-AMA exposure increased protein phosphorylation in a time-dependent alpha-AMA exposure. In addition, phosphorylation increased not only the components of the ERK signaling pathway but also U2AF65 and SPF45, known splicing factors. Therefore, we propose a novel mechanism of alpha-AMA as follows. The RAS/RAF/ERK signaling pathway involved in aberrant splicing events is activated by alpha-AMA exposure followed by aberrant splicing events leading to cell death in Huh-7 cells. | Kim, Doeun; Lee, Min Seo; Sung, Eunji; Lee, Sangkyu; Lee, Hye Suk | Kyungpook Natl Univ, Res Inst Pharmaceut Sci, Daegu 41566, South Korea; Catholic Univ Korea, Coll Pharm, BK21 Four Sponsored Adv Program SmartPharma Leade, Bucheon 14662, South Korea; Kyungpook Natl Univ, Coll Pharm, BK21 Four Community Based Intelligent Novel Drug, Daegu 41566, South Korea | Kim, Doeun/NJR-1829-2025 | 57219650718; 57225079010; 56988948200; 57209046767; 35316111800 | sangkyu@knu.ac.kr;sianalee@catholic.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 20 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.44 | 2025-06-25 | 4 | 5 | alpha-amanitin; toxic mushroom; acute liver failure; global phosphoproteome; RAS; RAF; ERK signaling pathway | ACTIVATED PROTEIN-KINASE; PHOSPHORYLATION; ERK; PROLIFERATION; MANAGEMENT; DIAGNOSIS; PATHWAYS; DEATH | acute liver failure; alpha-amanitin; global phosphoproteome; RAS/RAF/ERK signaling pathway; toxic mushroom | Alpha-Amanitin; MAP Kinase Signaling System; Phosphorylation; RNA Polymerase II; RNA Splicing Factors; RNA, Messenger; alpha amanitin; cadherin; histone H3; mammalian target of rapamycin; messenger RNA; mitogen activated protein kinase; mitogen activated protein kinase 1; mitogen activated protein kinase 3; mitogen activated protein kinase kinase 2; phosphopeptide; protein p53; protein tyrosine kinase; Raf protein; Ras protein; splicing factor U2AF; messenger RNA; RNA polymerase II; RNA splicing factor; acute liver failure; affinity chromatography; Article; bioinformatics; controlled study; drug cytotoxicity; enzyme phosphorylation; Huh-7 cell line; human; human cell; immunoblotting; in vitro study; liquid chromatography-mass spectrometry; MAPK signaling; mTOR signaling; poisonous mushroom; polyacrylamide gel electrophoresis; protein phosphorylation; RNA processing; RNA splicing; RNA transport; spliceosome; Western blotting; phosphorylation; physiology | English | 2022 | 2022-10 | 10.3390/ijms232012294 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Cirsilineol Treatment Attenuates PM2.5-Induced Lung Injury in Mice | Ultrafine particulate matter with less than 2.5 mu m diameter (PM2.5) is an air pollutant that causes severe lung damage. Currently, effective treatment and preventive methods for PM2.5-induced lung damage are limited. Cirsilineol (CSL) is a small natural compound isolated from Artemisia vestita. In this study, the efficacy of CSL on PM2.5-induced lung toxicity was tested, and its mechanism was identified. Lung injury was caused by intratracheal administration of PM2.5 suspension in animal models. Two days after PM2.5 pretreatment, CSL was injected via mouse tail vein for two days. The effects of CSL on PM2.5-induced lung damage, autophagy, apoptosis, and pulmonary inflammation in a mouse model and their mechanisms were investigated. CSL significantly suppressed histological lung damage and lung wet/dry weight proportion. CSL also significantly reduced PM2.5-induced autophagy dysfunction, apoptosis, lymphocyte suppression, and inflammatory cytokine levels in bronchoalveolar fluid (BALF). Furthermore, CSL increased mammalian target of rapamycin (mTOR) phosphorylation and significantly inhibited the expression of Toll-like receptors (TLR) 2 and 4, MyD88, and the autophagy proteins, Beclin1 and LC3II. Thus, CSL exerts protective effects on pulmonary damage by regulating mTOR and TLR2,4-myD88 autophagy pathways. Therefore, CSL can be used as an effective treatment for PM2.5-induced lung damage. | Kim, Chaeyeong; Kim, Go Oun; Bae, Jong-Sup | Kyungpook Natl Univ, Coll Pharm, Res Inst Pharmaceut Sci, Daegu 41566, South Korea | Bae, Jong-Sup/AAU-9724-2020 | 57418696700; 57896809800; 16021543200 | baejs@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 22 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.44 | 2025-06-25 | 5 | 5 | cirsilineol; particulate matter; lung toxicity; TLR2; 4-mTOR autophagy; apoptosis | FINE PARTICULATE MATTER; INDUCED INFLAMMATION; AUTOPHAGY; PM2.5; MTOR; ACTIVATION; HEALTH; APOPTOSIS; GROWTH; CANCER | 4–mTOR autophagy; apoptosis; cirsilineol; lung toxicity; particulate matter; TLR2 | Animals; Disease Models, Animal; Lung Injury; Mammals; Mice; Myeloid Differentiation Factor 88; Particulate Matter; TOR Serine-Threonine Kinases; antiinflammatory agent; beclin 1; cirsilineol; dexamethasone; gamma interferon; interleukin 10; interleukin 18; interleukin 1beta; interleukin 2; interleukin 4; interleukin 6; mammalian target of rapamycin; myeloid differentiation factor 88; plant medicinal product; toll like receptor 2; toll like receptor 4; tumor necrosis factor; unclassified drug; cirsilineol; myeloid differentiation factor 88; target of rapamycin kinase; animal cell; animal experiment; animal model; animal tissue; antiinflammatory activity; apoptosis; Article; autophagy (cellular); bronchoalveolar lavage fluid; controlled study; drug efficacy; drug mechanism; enzyme phosphorylation; histopathology; lung injury; lung parenchyma; lung toxicity; lung wet-dry weight ratio; lymphocyte; mouse; mTOR signaling; nonhuman; particulate matter 2.5; PM2.5 exposure; pneumonia; protein expression; suspension; tissue injury; animal; disease model; mammal; particulate matter; toxicity | English | 2022 | 2022-11 | 10.3390/ijms232213948 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Comparison of factors influencing fall recurrence in the young-old and old-old: a cross-sectional nationwide study in South Korea | Background Recurrent falls are a concerning problem in the elderly. Elderly people aged > 65 years who are prone to fall often require medical treatment for severe fall-related injuries, which is associated with a substantial financial burden. Therefore, this study aimed to identify factors related to recurrent falls in the community-dwelling young-old (65-74 years old) and old-old (>= 75 years) in South Korea. Methods This study used a cross-sectional, correlation design. Data from the 2017 National Survey of Older Koreans were used, and 5,838 young-old and 4,205 old-old elderly people were included in the analysis. The questionnaire included general characteristics, fall experience, physical status, mental status, and presence of chronic diseases. The data were analyzed using the chi-square test, one-way analysis of variance, and logistic regression analysis. Results In the young-old elderly people, limitations in activities of daily living (p < .001), use of visual aids (p = .002), cognitive function (p < .001), presence of suicidal ideations (p = .005), number of chronic diseases (p < .001), and number of prescribed medications used (p = .006) associated with fall recurrence. In the old-old elderly people, having a spouse (p = .034), being a beneficiary of the National Basic Livelihood Security System (p = .025), less exercise (p = .003), limitations in activities of daily living (p < .001), visual aid use (p = .002), presence of suicidal ideations (p = .015), number of chronic diseases (p < .001), and presence of Parkinson's disease (p < .001) associated with fall recurrence. Conclusions This study identified differences in factors related to fall recurrence between the young-old and old-old elderly. The results of this study indicate that it is necessary to implement an intervention program to prevent fall recurrence by age group in consideration of the risk factors for fall recurrence in each elderly people group. | Kim, Mi Young; Kim, Yujeong | Hanyang Univ, Coll Nursing, 222 Wangsimni Ro, Seoul 04763, South Korea; Kyungpook Natl Univ, Coll Nursing, Res Inst Nursing Sci, 680 Gukchabosangro, Daegu 41944, South Korea | 57069194800; 57200941945 | yujeongkim@knu.ac.kr; | BMC GERIATRICS | BMC GERIATR | 1471-2318 | 22 | 1 | SCIE;SSCI | GERIATRICS & GERONTOLOGY;GERONTOLOGY | 2022 | 4.1 | 23.0 | 0.83 | 2025-06-25 | 5 | 7 | Accidental falls; Aged; Frail elderly; Old-old; Young-old | ADVERSE DRUG-REACTIONS; RISK-FACTORS; PARKINSONS-DISEASE; POPULATIONS; STRATEGIES; PEOPLE; ADULTS; AGE | Accidental falls; Aged; Frail elderly; Old-old; Young-old | Activities of Daily Living; Aged; Cross-Sectional Studies; Humans; Independent Living; Republic of Korea; Risk Factors; aged; cross-sectional study; daily life activity; epidemiology; human; independent living; risk factor; South Korea | English | 2022 | 2022-06-25 | 10.1186/s12877-022-03172-7 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Complementary Regulation of BfmRS Two-Component and AbaIR Quorum Sensing Systems to Express Virulence-Associated Genes in Acinetobacter baumannii | Acinetobacter baumannii expresses various virulence factors to adapt to hostile environments and infect susceptible hosts. This study investigated the regulatory network of the BfmRS two-component and AbaIR quorum sensing (QS) systems in the expression of virulence-associated genes in A. baumannii ATCC 17978. The Delta bfmS mutant exhibited a significant decrease in surface motility, which presumably resulted from the low expression of pilT and A1S₀₁₁₂-A1S₀₁₁₉ gene cluster. The Delta bfmR mutant displayed a significant reduction in biofilm and pellicle formation due to the low expression of csu operon. The deletion of abaR did not affect the expression of bfmR or bfmS. However, the expression of abaR and abaI was upregulated in the Delta bfmR mutant. The Delta bfmR mutant also produced more autoinducers than did the wild-type strain, suggesting that BfmR negatively regulates the AbaIR QS system. The Delta bfmS mutant exhibited no autoinducer production in the bioassay system. The expression of the A1S₀₁₁₂-A1S₀₁₁₉ gene cluster was downregulated in the Delta abaR mutant, whereas the expression of csu operon was upregulated in this mutant with a high cell density. In conclusion, for the first time, we demonstrated that the BfmRS-AbaIR QS system axis regulated the expression of virulence-associated genes in A. baumannii. This study provides new insights into the complex network system involved in the regulation of virulence-associated genes underlying the pathogenicity of A. baumannii. | Kim, Hyo-Jeong; Kim, Na-Yeong; Ko, Seo-Yeon; Park, Seong-Yong; Oh, Man-Hwan; Shin, Min-Sang; Lee, Yoo-Chul; Lee, Je-Chul | Kyungpook Natl Univ, Sch Med, Dept Microbiol, Daegu 41944, South Korea; Dankook Univ, Coll Sci & Technol, Dept Microbiol, Cheonan 16890, South Korea | Park, Seong Yong/IZQ-1385-2023; Lee, JongGu/B-7384-2013 | 57221772924; 57211500281; 57959870800; 57958559800; 57113010400; 7401536650; 8710443700; 25930392000 | leejc@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 23 | 21 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 1.68 | 2025-06-25 | 20 | 19 | Acinetobacter baumannii; virulence factor; two-component system; quorum sensing system; BfmRS | RESISTANCE | Acinetobacter baumannii; BfmRS; quorum sensing system; two-component system; virulence factor | Acinetobacter baumannii; Bacterial Proteins; Biofilms; Gene Expression Regulation, Bacterial; Quorum Sensing; Virulence; bacterial protein; Acinetobacter baumannii; Article; bacterial capsule; bacterial cell; bacterial gene; bacterial growth; bacterial virulence; bacterium mutant; biofilm; cell density; controlled study; down regulation; gene cluster; gene deletion; gene expression; gene regulatory network; nonhuman; operon; quorum sensing; upregulation; wild type; gene expression regulation; genetics; metabolism; quorum sensing; virulence | English | 2022 | 2022-11 | 10.3390/ijms232113136 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Comprehensive Genome-Wide Analysis and Expression Pattern Profiling of the SlHVA22 Gene Family Unravels Their Likely Involvement in the Abiotic Stress Adaptation of Tomato | HVA22 family proteins with a conserved TB2/DP1/HVA22 domain are ubiquitous in eukaryotes. HVA22 family genes have been identified in a variety of plant species. However, there has been no comprehensive genome-wide analysis of HVA22 family genes in tomato (Solanum lycopersicum L.). Here, we identified 15 non-redundant SlHVA22 genes with three segmentally duplicated gene pairs on 8 of the 12 tomato chromosomes. The predicted three-dimensional (3D) models and gene ontology (GO) annotations of SlHVA22 proteins pointed to their putative transporter activity and ability to bind to diverse ligands. The co-expression of SlHVA22 genes with various genes implicated in multiple metabolic pathways and the localization of SlHVA22-GFP fused proteins to the endoplasmic reticulum suggested that they might have a variety of biological functions, including vesicular transport in stressed cells. Comprehensive expression analysis revealed that SlHVA22 genes were differentially expressed in various organs and in response to abiotic stress conditions. The predominant expression of SlHVA22i at the ripening stage and that of SlHVA22g, SlHVA22k, and SlHVA22l in fruits at most developmental stages suggested their probable involvement in tomato fruit development and ripening. Moreover, the transcript expression of most tomato HVA22 genes, particularly SlHVA22b, SlHVA22i, SlHVA22k, SlHVA22l, SlHVA22m, and SlHVA22n, was affected by abscisic acid (ABA) and diverse abiotic stress treatments, indicating the likely involvement of these genes in tomato abiotic stress responses in an ABA-dependent manner. Overall, our findings provide a foundation to better understand the structures and functional roles of SlHVA22 genes, many of which might be useful to improve the abiotic stress tolerance and fruit quality of tomato through marker-assisted backcrossing or transgenic approaches. | Wai, Antt Htet; Waseem, Muhammad; Cho, Lae-Hyeon; Kim, Sang-Tae; Lee, Do-jin; Kim, Chang-Kil; Chung, Mi-Young | Sunchon Natl Univ, Dept Agr Educ, 413 Jungangno, Sunchon 57922, South Korea; Yangon Univ Educ, Dept Biol, Kamayut Township 11041, Yangon, Myanmar; Univ Narowal, Dept Bot, Narowal 51600, Pakistan; Pusan Natl Univ, Coll Nat Resources & Life Sci, Dept Plant Biosci, Miryang Si 50463, South Korea; Catholic Univ Korea, Dept Med & Biol Sci, Bucheon 14662, South Korea; Kyungpook Natl Univ, Dept Hort, Daegu 41566, South Korea | ; Kim, Sang-Tae/X-8917-2019; Wai, Antt/AAG-9099-2021; Kim, Sang-Tae/U-9457-2018; WASEEM, MUHAMMAD/K-9284-2019; Waseem, Muhammad/C-8001-2015 | 57196010294; 57203320375; 35110429900; 57202352361; 7406659787; 7409880701; 24821361600 | queen@sunchon.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 23 | 20 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.8 | 2025-06-25 | 10 | 9 | HVA22 genes; Solanum lycopersicum; stress response; subcellular localization; gene co-expression network; 3D structure; expression analysis | ABSCISIC-ACID; TRANSCRIPTION FACTORS; PROTEIN-PHOSPHORYLATION; REGULATORY ELEMENTS; MEMBRANE-PROTEINS; WRKY GENE; PLANT; REVEALS; RICE; IDENTIFICATION | 3D structure; expression analysis; gene co-expression network; HVA22 genes; Solanum lycopersicum; stress response; subcellular localization | Abscisic Acid; Gene Expression Profiling; Gene Expression Regulation, Plant; Genome, Plant; Lycopersicon esculentum; Multigene Family; Phylogeny; Plant Proteins; Stress, Physiological; abscisic acid; cis acting element; hva22 protein; microRNA; plant protein; unclassified drug; plant protein; abiotic stress; adaptation; Article; comparative study; controlled study; developmental stage; duplicate gene; fruit development; fruit ripening; gene duplication; gene expression profiling; gene expression regulation; gene ontology; gene structure; genetic conservation; hormonal regulation; multigene family; nonhuman; nucleotide motif; plant chromosome; plant metabolism; protein domain; protein phosphorylation; sequence alignment; structural homology; synteny; tomato; vesicle trafficking; gene expression profiling; genetics; metabolism; multigene family; phylogeny; physiological stress; plant genome | English | 2022 | 2022-10 | 10.3390/ijms232012222 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Effect of Paper-Bagging on Apple Skin Patterning Associated with MdMYB10 Promoter Methylation | Paper-bagging is an efficient method to maximize apple skin color, but a relationship between this technique and fruit skin patterning has not been demonstrated. Here, the 'Fuji' fruit with red-striped skin changed to red-blushed skin under re-exposure to light after bag treatment. Higher expression of MdMYB10, a transcription factor that regulates anthocyanin biosynthesis in apples, correlated with increased anthocyanin concentration in bag removal fruit. At the mature stage, a comparison of methylation status in the MdMYB10 promoter revealed that the methylation level in the region from -2585 to -2117 bp was reduced in bag removal fruit, especially for CHG context. It can be regulated by the downregulated expression of DNA methyltransferases such as MdMET, MdCMT, and MdDRM. Our results suggest that the bag removal treatment in this cultivar causes a change in skin patterning from striped to blushed pigmentation by inducing DNA demethylation of MdMYB10. | Cho, Hye Jeong; Han, A. Reum; Choi, Cheol | Kyungpook Natl Univ, Coll Agr & Life Sci, 80 Daehak Ro, Daegu 41566, South Korea | 57211313145; 57208301535; 50261314300 | hyejeong725@naver.com;har0903@naver.com;cc31@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 6 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.71 | 2025-06-25 | 7 | 8 | anthocyanin; apple; bag treatment; DNA methylation; MYB transcription factor; skin patterning | ANTHOCYANIN BIOSYNTHESIS; FRUIT-QUALITY; COLOR; EXPRESSION; ACCUMULATION; MATURITY; GENES; IRRADIATION; PLANT; TOOL | Anthocyanin; Apple; Bag treatment; DNA methylation; MYB transcription factor; Skin patterning | Anthocyanins; DNA Methylation; Fruit; Gene Expression Regulation, Plant; Malus; Plant Proteins; complementary DNA; DNA methyltransferase; anthocyanin; plant protein; apple; Article; biosynthesis; comparative study; DNA extraction; DNA methylation; down regulation; epigenetic modification; food handling; fruit color; mdmyb10 gene; nonhuman; paper bagging; pigmentation; promoter region; protein expression; real time polymerase chain reaction; regulator gene; RNA extraction; structural gene; DNA methylation; fruit; gene expression regulation; genetics; Malus; metabolism | English | 2022 | 2022-03 | 10.3390/ijms23063319 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Effects of the Rhizosphere Fungus Cunninghamella bertholletiae on the Solanum lycopersicum Response to Diverse Abiotic Stresses | This study examined the efficiency of fungal strain (Cunninghamella bertholletiae) isolated from the rhizosphere of Solanum lycopersicum to reduce symptoms of salinity, drought and heavy metal stresses in tomato plants. In vitro evaluation of C. bertholletiae demonstrated its ability to produce indole-3-Acetic Acid (IAA), ammonia and tolerate varied abiotic stresses on solid media. Tomato plants at 33 days' old, inoculated with or without C. bertholletiae, were treated with 1.5% sodium chloride, 25% polyethylene glycol, 3 mM cadmium and 3 mM lead for 10 days, and the impact of C. bertholletiae on plant performance was investigated. Inoculation with C. bertholletiae enhanced plant biomass and growth attributes in stressed plants. In addition, C. bertholletiae modulated the physiochemical apparatus of stressed plants by raising chlorophyll, carotenoid, glucose, fructose, and sucrose contents, and reducing hydrogen peroxide, protein, lipid metabolism, amino acid, antioxidant activities, and abscisic acid. Gene expression analysis showed enhanced expression of SlCDF3 and SlICS genes and reduced expression of SlACCase, SlAOS, SlGRAS6, SlRBOHD, SlRING1, SlTAF1, and SlZH13 genes following C. bertholletiae application. In conclusion, our study supports the potential of C. bertholletiae as a biofertilizer to reduce plant damage, improve crop endurance and remediation under stress conditions. | Kazerooni, Elham Ahmed; Maharachchikumbura, Sajeewa S. N.; Al-Sadi, Abdullah Mohammed; Rashid, Umer; Kim, Il-Doo; Kang, Sang-Mo; Lee, In-Jung | Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea; Univ Elect Sci & Technol China, Ctr Informat Biol, Sch Life Sci & Technol, Chengdu 611731, Peoples R China; Sultan Qaboos Univ, Coll Agr & Marine Sci, Dept Plant Sci, POB 34, Al Khoud 123, Oman; Univ Putra Malaysia, Inst Nanosci & Nanotechnol ION2, Serdang 43400, Selangor, Malaysia | Maharachchikumbura, Sajeewa/C-9403-2013; Kim, Il-Doo/C-1850-2011; maharachchikumbura, sajeewa/C-9403-2013; Al-Sadi, Abdullah/D-6766-2012; Kang, Sang-Mo/MBG-7823-2025; Rashid, Umer/AGW-1380-2022; Lee, In-Jung/GLS-0432-2022 | 57191375873; 54385665100; 8602920100; 16031556400; 56269995600; 56189696900; 16425830900 | elham.ghasemi.k@gmail.com;ijlee@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 16 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.8 | 2025-06-25 | 10 | 9 | tomato; salinity; heavy metal; drought; antioxidant enzymes; sugar; phytohormone | GROWTH-PROMOTING FUNGUS; TRANSCRIPTION FACTOR; DROUGHT STRESS; SALT STRESS; GENE-EXPRESSION; ANTIOXIDANT ACTIVITY; ACETYL-COENZYME; SALINITY STRESS; SALICYLIC-ACID; SOLUBLE SUGAR | antioxidant enzymes; drought; heavy metal; phytohormone; salinity; sugar; tomato | Cunninghamella; Lycopersicon esculentum; Rhizosphere; Stress, Physiological; cadmium; carotenoid; catalase; chlorophyll; fructose; glucose; hydrogen peroxide; indoleacetic acid; macrogol; malonaldehyde; phytohormone; reactive oxygen metabolite; sodium chloride; sucrose; sugar; superoxide dismutase; ubiquitin protein ligase E3; abiotic stress; antioxidant activity; Article; comparative study; controlled study; Cunninghamella bertholletiae; drought; fungal strain; gene expression profiling; in vitro study; lipid metabolism; nonhuman; physiological stress; plant growth; rhizosphere fungus; salinity; tomato; Cunninghamella; genetics; rhizosphere | English | 2022 | 2022-08 | 10.3390/ijms23168909 | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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