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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | ○ | Article | Expression of Piezo1 in the Trigeminal Neurons and in the Axons That Innervate the Dental Pulp | Information on the neurons and axons that express the mechanosensitive channel Piezo1 and its expression in axons innervating the dental pulp may help understand the nature of the Piezo1-mediated mechanosensation and the underlying mechanism of dentin sensitivity elicited by mechanical stimuli. For this, we here investigated the neurochemical properties of the neurons in the rat trigeminal ganglion (TG) and their axons in its sensory root that express Piezo1 and the expression of Piezo1 in the rat and human dental pulp by light and electron microscopic immunohistochemistry and quantitative analysis. Piezo1 was expressed mainly in medium-sized and large TG neurons. Piezo1-immunopositive (+) neurons frequently coexpressed the marker for neurons with myelinated axons, NF200, but rarely the markers for neurons with unmyelinated axons, CGRP or IB4. In the sensory root of TG, Piezo1 was expressed primarily in small myelinated axons (A delta, 60.2%) but also in large myelinated (A beta, 24.3%) and unmyelinated (C, 15.5%) axons. In the human dental pulp, Piezo1 was expressed in numerous NF200+ axons, which formed a network in the peripheral pulp and often "ascended" toward the dentin. Most Piezo1+ myelinated axons in the radicular pulp became unmyelinated in the peripheral pulp, where Piezo1 immunoreaction product was associated with the axonal plasma membrane, suggesting a functional role of Piezo1 in the peripheral pulp. These findings suggest that Piezo1 is involved primarily in mediating the acute pain elicited by high-threshold mechanical stimuli, and that the Piezo1-mediated dental mechanotransduction occurs primarily in the axons in the peripheral pulp. | Cho, Yi Sul; Han, Hye Min; Jeong, Soon Youn; Kim, Tae Heon; Choi, So Young; Kim, Yun Sook; Bae, Yong Chul | Kyungpook Natl Univ, Sch Dent, Dept Anat & Neurobiol, Daegu, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Oral & Maxillofacial Surg, Daegu, South Korea | Kim, Tae/C-5935-2015 | 23979595700; 57204187581; 57802512300; 56160152900; 57202918688; 23037541600; 56377838800 | ycbae@knu.ac.kr; | FRONTIERS IN CELLULAR NEUROSCIENCE | FRONT CELL NEUROSCI | 1662-5102 | 16 | SCIE | NEUROSCIENCES | 2022 | 5.3 | 22.6 | 1.27 | 2025-06-25 | 12 | 14 | Piezo1; mechanosensitive channel; dentin sensitivity; ultrastructure; dental pulp | ION CHANNELS; FLUID-FLOW; PAIN; CELLS; TOUCH; ROOT | dental pulp; dentin sensitivity; mechanosensitive channel; Piezo1; ultrastructure | calcitonin gene related peptide; fluorescein isothiocyanate; ion channel; isolectin B4; peptides and proteins; peroxidase; piezo1; unclassified drug; adult; animal experiment; animal model; animal tissue; Article; axon; dentin; dentin hypersensitivity; electron microscopy; human; human tissue; immunofluorescence assay; immunohistochemistry; immunoreactivity; male; mechanical stimulation; mechanosensing; mechanotransduction; microscopy; myelinated nerve; neurochemistry; neurofilament; nonhuman; nonmyelinated nerve; orthodontic procedure; pain; premolar tooth; protein expression; quantitative analysis; rat; sensory nerve cell; tissue preparation; tooth pulp; trigeminal nerve; trigeminus ganglion | English | 2022 | 2022-06-29 | 10.3389/fncel.2022.945948 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Review | From 2D to 4D Phase-Contrast MRI in the Neurovascular System: Will It Be a Quantum Jump or a Fancy Decoration? | Considering the crosstalk between the flow and vessel wall, hemodynamic assessment of the neurovascular system may offer a well-integrated solution for both diagnosis and management by adding prognostic significance to the standard CT/MR angiography. 4D flow MRI or time-resolved 3D velocity-encoded phase-contrast MRI has long been promising for the hemodynamic evaluation of the great vessels, but challenged in clinical studies for assessing intracranial vessels with small diameter due to long scan times and low spatiotemporal resolution. Current accelerated MRI techniques, including parallel imaging with compressed sensing and radial k-space undersampling acquisitions, have decreased scan times dramatically while preserving spatial resolution. 4D flow MRI visualized and measured 3D complex flow of neurovascular diseases such as aneurysm, arteriovenous shunts, and atherosclerotic stenosis using parameters including flow volume, velocity vector, pressure gradients, and wall shear stress. In addition to the noninvasiveness of the phase contrast technique and retrospective flow measurement through the wanted windows of the analysis plane, 4D flow MRI has shown several advantages over Doppler ultrasound or computational fluid dynamics. The evaluation of the flow status and vessel wall can be performed simultaneously in the same imaging modality. This article is an overview of the recent advances in neurovascular 4D flow MRI techniques and their potential clinical applications in neurovascular disease. Level of Evidence 5 Technical Efficacy Stage 3 | Youn, Sung Won; Lee, Jongmin | Catholic Univ Daegu, Dept Radiol, Sch Med, Daegu, South Korea; Kyungpook Natl Univ, Dept Radiol & Biomed Engn, Sch Med, Daegu, South Korea | Youn, Sung-Won/AAH-1723-2020 | 57206373939; 55689919700 | jonglee@knu.ac.kr; | JOURNAL OF MAGNETIC RESONANCE IMAGING | J MAGN RESON IMAGING | 1053-1807 | 1522-2586 | 55 | 2 | SCIE | RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING | 2022 | 4.4 | 22.6 | 1.53 | 2025-06-25 | 23 | 19 | 4D flow MRI; time‐ resolved; velocity‐ encoding; neurovascular disease; intracranial flow; cerebral aneurysm | WALL SHEAR-STRESS; INTRA-ANEURYSMAL FLOW; 3D BLOOD-FLOW; K-T BLAST; CEREBRAL ARTERIOVENOUS-MALFORMATIONS; COMPUTATIONAL FLUID-DYNAMICS; CAROTID-ARTERY STENOSIS; IN-VIVO ASSESSMENT; INTRACRANIAL ANEURYSMS; ENDOVASCULAR EMBOLIZATION | Aneurysm; Blood Flow Velocity; Hemodynamics; Humans; Hydrodynamics; Imaging, Three-Dimensional; Magnetic Resonance Imaging; Retrospective Studies; contrast medium; atherosclerosis; brain arteriovenous malformation; brain artery aneurysm; cerebrovascular disease; computational fluid dynamics; Doppler flowmetry; four-dimensional imaging; human; information processing; nuclear magnetic resonance imaging; phase contrast imaging; pressure gradient; Review; shear stress; treatment response; two-dimensional imaging; aneurysm; blood flow velocity; hemodynamics; hydrodynamics; retrospective study; three-dimensional imaging | English | 2022 | 2022-02 | 10.1002/jmri.27430 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Neuroinflammation Induced by Transgenic Expression of Lipocalin-2 in Astrocytes | Transgenic mice are a useful tool for exploring various aspects of gene function. A key element of this approach is the targeted overexpression of specific genes in cells or tissues. Herein, we report for the first time, the generation and characterization of conditional transgenic (cTg) mice for lipocalin-2 (LCN2) expression. We generated the R26-LCN2-transgenic (LCN2-cTg) mice that carried a loxP-flanked STOP (neo) cassette, Lcn2 cDNA, and a GFP sequence. When bred with Tg mice expressing Cre recombinase under the control of various tissues or cell-specific promoters, Cre-mediated recombination deletes the STOP cassette and allows the expression of LCN2 and GFP. In this study, we achieved the recombination of loxP-flanked LCN2 in hippocampal astrocytes of cTg mouse brain, using a targeted delivery of adeno-associated virus (AAVs) bearing Cre recombinase under the control of a GFAP promoter (AAVs-GFAP-mCherry-Cre). These mice with localized LCN2 overexpression in astrocytes of the hippocampus developed neuroinflammation with enhanced glial activation and increased mRNA and protein levels of proinflammatory cytokines. Furthermore, mice showed impairment in cognitive functions as a typical symptom of hippocampal inflammation. Taken together, our study demonstrates the usefulness of LCN2-cTg mice in targeting specific cells at various organs for conditional LCN2 expression and for subsequent investigation of the functional role of cell-type-specific LCN2 within these sites. Moreover, the LCN2-cTg mice with targeted expression of LCN2 in hippocampal astrocytes are a new in vivo model of neuroinflammation. | Kim, Jae-Hong; Kwon, Osung; Bhusal, Anup; Lee, Jiyoun; Hwang, Eun Mi; Ryu, Hoon; Park, Jae-Yong; Suk, Kyoungho | Kyungpook Natl Univ, Dept Pharmacol, Daegu, South Korea; Kyungpook Natl Univ, Sch Med, Dept Biomed Sci, Daegu, South Korea; Kyungpook Natl Univ, Brain Sci & Engn Inst, Daegu, South Korea; Korea Univ, Grad Sch, Dept Integrated Biomed & Life Sci, Seoul, South Korea; Korea Univ, FOUR R&E Ctr Learning Hlth Syst BK21, Seoul, South Korea; Korea Inst Sci & Technol, Brain Sci Inst, Ctr Funct Connect, Seoul, South Korea; Korea Inst Sci & Technol, Brain Sci Inst, Ctr Neurosci, Seoul, South Korea; Vet Affairs Boston Healthcare Syst, Boston, MA USA; Boston Univ, Alzheimers Dis Ctr, Boston, MA 02215 USA; Boston Univ, Sch Med, Dept Neurol, Boston, MA 02215 USA | ; Ryu, Hoon/MCJ-9411-2025; Hwang, Eun Mi/HHN-3030-2022 | 55926599800; 56577095600; 57200274141; 57255852300; 7101826741; 7202277209; 57206479071; 7005114595 | jaeyong68@korea.ac.kr;ksuk@knu.ac.kr; | FRONTIERS IN CELLULAR NEUROSCIENCE | FRONT CELL NEUROSCI | 1662-5102 | 16 | SCIE | NEUROSCIENCES | 2022 | 5.3 | 22.6 | 1.09 | 2025-06-25 | 12 | 12 | transgenic mice; astrocytes; lipocalin-2; neuroinflammation; hippocampus | BRAIN-INJURY; HIPPOCAMPAL; DYSFUNCTION; VECTOR; MEMORY | astrocytes; hippocampus; lipocalin-2; neuroinflammation; transgenic mice | green fluorescent protein; interleukin 1beta; neutrophil gelatinase associated lipocalin; tumor necrosis factor; Adeno associated virus; adult; animal cell; animal experiment; animal model; animal tissue; Article; astrocyte; cognitive defect; controlled study; electroporation; enzyme linked immunosorbent assay; female; gene expression; gene overexpression; genetic transfection; hippocampus; immunofluorescence; immunoreactivity; in vivo study; male; microdialysis; Morris water maze test; mouse; nervous system inflammation; nonhuman; novel object recognition test; passive avoidance test; protein expression; real time polymerase chain reaction; transgenic mouse; Western blotting; whole genome sequencing; working memory | English | 2022 | 2022-02-23 | 10.3389/fncel.2022.839118 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Pericyte Loss Leads to Capillary Stalling Through Increased Leukocyte-Endothelial Cell Interaction in the Brain | The neurovascular unit is a functional unit composed of neurons, glial cells, pericytes, and endothelial cells which sustain brain activity. While pericyte is a key component of the neurovascular unit, its role in cerebral blood flow regulation remains elusive. Recently, capillary stalling, which means the transient interruption of microcirculation in capillaries, has been shown to have an outsized impact on microcirculatory changes in several neurological diseases. In this study, we investigated capillary stalling and its possible causes, such as the cerebral endothelial glycocalyx and leukocyte adhesion molecules after depleting pericytes postnatally in mice. Moreover, we investigated hypoxia and gliosis as consequences of capillary stalling. Although there were no differences in the capillary structure and RBC flow, longitudinal optical coherence tomography angiography showed an increased number of stalled segments in capillaries after pericyte loss. Furthermore, the extent of the cerebral endothelial glycocalyx was decreased with increased expression of leukocyte adhesion molecules, suggesting enhanced interaction between leukocytes and endothelial cells. Finally, pericyte loss induced cerebral hypoxia and gliosis. Cumulatively, the results suggest that pericyte loss induces capillary stalling through increased interaction between leukocytes and endothelial cells in the brain. | Choe, Young-Geun; Yoon, Jin-Hui; Joo, Jongyoon; Kim, Bokyung; Hong, Seon Pyo; Koh, Gou Young; Lee, Dong-Seok; Oh, Wang-Yuhl; Jeong, Yong | Korea Adv Inst Sci & Technol, Dept Bio & Brain Engn, Daejeon, South Korea; Korea Adv Inst Sci & Technol, KI Hlth Sci & Technol, Daejeon, South Korea; Inst for Basic Sci Korea, Ctr Vasc Res, Daejeon, South Korea; Korea Adv Inst Sci & Technol, Dept Mech Engn, Daejeon, South Korea; Kyungpook Natl Univ, Coll Nat Sci, Sch Life Sci & Biotechnol, Daegu, South Korea; Kyungpook Natl Univ, Sch Life Sci, BK21 FOUR KNU Creat Biores Grp, Daegu, South Korea; Korea Adv Inst Sci & Technol, Grad Sch Med Sci & Engn, Daejeon, South Korea | Koh, Gou/C-1615-2011; Yoon, Jin-Hui/LIH-3735-2024; Jeong, Yong/C-1677-2011; Oh, Wang-Yuhl/C-2055-2011 | 57562595300; 57193844963; 57202188071; 57205869587; 57202222148; 20234840600; 57210068061; 24438242500; 7202332061 | woh1@kaist.ac.kr;yong@kaist.ac.kr; | FRONTIERS IN CELLULAR NEUROSCIENCE | FRONT CELL NEUROSCI | 1662-5102 | 16 | SCIE | NEUROSCIENCES | 2022 | 5.3 | 22.6 | 1.73 | 2025-06-25 | 18 | 19 | capillary stalling; pericyte; leukocyte-endothelial cell interaction; cerebral endothelial glycocalyx; leukocyte adhesion molecules | BLOOD-FLOW; NEUROVASCULAR UNIT; GLYCOCALYX; HOMEOSTASIS; MIGRATION; ADHESION; HEALTH | capillary stalling; cerebral endothelial glycocalyx; leukocyte adhesion molecules; leukocyte-endothelial cell interaction; pericyte | 3 phosphoadenosine 5 phosphosulfate synthase 2; angiopoietin; angiopoietin 2; angiopoietin receptor; beta 1 4 galactosyltransferase 3; biglycan; carrier proteins and binding proteins; corn oil; decorin; diphtheria toxin; glial fibrillary acidic protein; glyceraldehyde 3 phosphate dehydrogenase; glycosyltransferase; glypican 3; immunoglobulin enhancer binding protein; intercellular adhesion molecule 1; isoflurane; L selectin; microsphere; solute carrier family 35 member b3; sulfotransferase; t5648; tamoxifen; tiletamine plus zolazepam; unclassified drug; vascular cell adhesion molecule 1; xylazine; animal cell; animal experiment; animal model; animal tissue; area under the curve; Article; blood brain barrier; blood flow velocity; blood volume; brain; brain blood flow; brain hypoxia; brain pericyte; C57BL mouse; capillary stalling; cell interaction; cell loss; confocal microscopy; controlled study; differential gene expression; endothelium cell; erythrocyte; female; gene set enrichment analysis; gliosis; glycocalyx; high throughput sequencing; immunohistochemistry; leukocyte; leukocyte adherence; male; microcirculation; mouse; mouse strain; multiphoton microscopy; neurovascular coupling; nonhuman; optical coherence tomography angiography; oxygen saturation; Pdgfr beta CreER mouse; pulse oximetry; reverse transcription; RNA isolation; RNA sequence; RNA sequencing; ROSA26 DTA mouse; spectrophotometry; vascular disease; Western blotting | English | 2022 | 2022-03-11 | 10.3389/fncel.2022.848764 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Ultrastructure of Rat Rostral Nucleus of the Solitary Tract Terminals in the Parabrachial Nucleus and Medullary Reticular Formation | Neurons in the rostral nucleus of the solitary tract (rNST) receive taste information from the tongue and relay it mainly to the parabrachial nucleus (PBN) and the medullary reticular formation (RF) through two functionally different neural circuits. To help understand how the information from the rNST neurons is transmitted within these brainstem relay nuclei in the taste pathway, we examined the terminals of the rNST neurons in the PBN and RF by use of anterograde horseradish peroxidase (HRP) labeling, postembedding immunogold staining for glutamate, serial section electron microscopy, and quantitative analysis. Most of the anterogradely labeled, glutamate-immunopositive axon terminals made a synaptic contact with only a single postsynaptic element in PBN and RF, suggesting that the sensory information from rNST neurons, at the individual terminal level, is not passed to multiple target cells. Labeled terminals were usually presynaptic to distal dendritic shafts in both target nuclei. However, the frequency of labeled terminals that contacted dendritic spines was significantly higher in the PBN than in the RF, and the frequency of labeled terminals that contacted somata or proximal dendrites was significantly higher in the RF than in the PBN. Labeled terminals receiving axoaxonic synapses, which are a morphological substrate for presynaptic modulation frequently found in primary sensory afferents, were not observed. These findings suggest that the sensory information from rNST neurons is processed in a relatively simple manner in both PBN and RF, but in a distinctly different manner in the PBN as opposed to the RF. | Park, Sook Kyung; Cho, Yi Sul; Kim, Jong Ho; Kim, Yun Sook; Bae, Yong Chul | Kyungpook Natl Univ, Sch Dent, Dept Anat & Neurobiol, Daegu, South Korea | 37099111300; 23979595700; 57195453960; 23037541600; 56377838800 | ycbae@knu.ac.kr; | FRONTIERS IN CELLULAR NEUROSCIENCE | FRONT CELL NEUROSCI | 1662-5102 | 16 | SCIE | NEUROSCIENCES | 2022 | 5.3 | 22.6 | 0.18 | 2025-06-25 | 2 | 2 | gustatory; rNST neurons; synapse; central connectivity; ultrastructure | PRIMARY AFFERENT TERMINALS; AXON TERMINALS; GABA; GLYCINE; IMMUNOREACTIVITY; CONNECTIVITY; EXPRESSION; PLASTICITY; MORPHOLOGY; GLUTAMATE | central connectivity; gustatory; rNST neurons; synapse; ultrastructure | amino acid; antiserum; diaminobenzidine; glutamic acid; horseradish peroxidase; tetramethylbenzidine; animal experiment; animal model; animal tissue; Article; dendrite; dendritic spine; electron microscopy; embedding; immunogold staining; immunohistochemistry; immunoreactivity; male; medullary reticular formation; nerve ending; nonhuman; parabrachial nucleus; quantitative analysis; rat; solitary tract nucleus; ultrastructure | English | 2022 | 2022-03-17 | 10.3389/fncel.2022.858617 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Zinc enhances autophagic flux and lysosomal function through transcription factor EB activation and V-ATPase assembly | The stimulation of autophagy or lysosomes has been considered therapeutic for neurodegenerative disorders because the accumulation of misfolded proteins is commonly observed in the brains of individuals with these diseases. Although zinc is known to play critical roles in the functions of lysosomes and autophagy, the mechanism behind this regulatory relationship remains unclear. Therefore, in this study, we examined which mechanism is involved in zinc-mediated activation of autophagy and lysosome. Exposure to zinc at a sub-lethal concentration activated autophagy in a concentration-dependent manner in mRFP-GFP-LC3-expressing H4 glioma cells. Zinc also rescued the blocking of autophagic flux arrested by pharmaceutical de-acidification. Co-treatment with zinc attenuated the chloroquine (CQ)-induced increase in the number and size of mRFP-GFP-LC3 puncta in H4 cells and accumulation of p62 by CQ or ammonium chloride in both H4 and mouse cerebrocortical cultures. Zinc rapidly induced the expression of cathepsin B (CTSB) and cathepsin D (CTSD), representative lysosomal proteases in neurons, which appeared likely to be mediated by transcription factor EB (TFEB). We observed the translocation of TFEB from neurite to nucleus and the dephosphorylation of TFEB by zinc. The addition of cycloheximide, a chemical inhibitor of protein synthesis, inhibited the activity of CTSB and CTSD at 8 h after zinc exposure but not at 1 h, indicating that only late lysosomal activation was dependent on the synthesis of CTSB and CTSD proteins. At the very early time point, the activation of cathepsins was mediated by an increased assembly of V-ATPase on lysosomes and resultant lysosomal acidification. Finally, considering that P301L mutation in tau protein causes frontotemporal dementia through aggressive tau accumulation, we investigated whether zinc reduces the accumulation of protein aggregates in SK-N-BE(2)-C neuroblastoma cells expressing wild-type tau or mutant P301L-tau. Zinc markedly attenuated the levels of phosphorylated tau and total tau as well as p62 in both wild-type and mutant tau-overexpressing cells. We also observed that zinc was more effective than rapamycin at inducing TFEB-dependent CTSB and CTSD expression and V-ATPase-dependent lysosomal acidification and CTSB/CTSD activation. These results suggest that the regulation of zinc homeostasis could be a new approach for developing treatments for neurodegenerative diseases, including Alzheimer's and Parkinson's. | Kim, Ki-Ryeong; Park, Sang Eun; Hong, Ji-Ye; Koh, Jae-Young; Cho, Dong-Hyung; Hwang, Jung Jin; Kim, Yang-Hee | Sejong Univ, Dept Integrat Biosci & Biotechnol, Seoul, South Korea; Asan Med Ctr, Asan Inst Life Sci, Seoul, South Korea; Univ Ulsan, Coll Med, Asan Med Ctr, Dept Neurol, Seoul, South Korea; Asan Med Ctr, Biomed Res Ctr, Asan Inst Life Sci, Neuronal Injury Lab, Seoul, South Korea; Kyungpook Natl Univ, Sch Life Sci, FOUR KNU Creat BioRes Grp BK21, Daegu, South Korea | Choi, Hye Rin/JDV-9065-2023; Kim, Yang-Hee/N-7614-2013 | 56544774000; 57191671783; 57224948144; 7201756953; 35093684400; 7403897177; 18042053200 | jjhwang@amc.seoul.kr;yhkim@sejong.ac.kr; | FRONTIERS IN CELLULAR NEUROSCIENCE | FRONT CELL NEUROSCI | 1662-5102 | 16 | SCIE | NEUROSCIENCES | 2022 | 5.3 | 22.6 | 1.09 | 2025-06-25 | 10 | 12 | zinc; lysosome; autophagy; TFEB; cathepsin B; cathepsin D; V-ATPase; neurodegenerative disease | INTRACELLULAR ZINC; ALZHEIMERS-DISEASE; CELL-DEATH; APOPTOSIS; TFEB; DEPLETION; RELEASE; PROTEIN; REGULATOR; INCREASES | autophagy; cathepsin B; cathepsin D; lysosome; neurodegenerative disease; TFEB; V-ATPase; zinc | adenosine triphosphatase; ammonium chloride; cathepsin B; cathepsin D; chloroquine; cycloheximide; proteinase; sequestosome 1; tau protein; transcription factor; transcription factor eb; unclassified drug; vacuolar type adenosine triphosphatase; zinc; Alzheimer disease; animal cell; animal tissue; Article; autophagy (cellular); brain cortex; cell culture; cerebral cortical tissue; chemical reaction; confocal laser scanning microscopy; confocal microscopy; controlled study; deacidification; degenerative disease; dephosphorylation; enzyme activity; fluorescence microscopy; frontotemporal dementia; gene mutation; gene overexpression; genetic transfection; H4 cell line; human; human cell; image analysis; immunoblotting; immunochemistry; lysosome; mouse; nonhuman; Parkinson disease; polyacrylamide gel electrophoresis; protein determination; protein phosphorylation; protein synthesis; reverse transcription polymerase chain reaction; RNA extraction; SK-N-BE(2)-C cell line; statistical analysis; Western blotting; wild type; zinc homeostasis | English | 2022 | 2022-09-29 | 10.3389/fncel.2022.895750 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | The Relationship Between Employees' Daily Customer Injustice and Customer-Directed Sabotage: Cross-Level Moderation Effects of Emotional Stability and Attentiveness | Customer injustice has received considerable attention in the field of organizational behavior because it generates a variety of negative outcomes. Among possible negative consequences, customer-directed sabotage is the most common reaction, which impacts individuals' well-being and the prosperity of organizations. To minimize such negative consequences, researchers have sought to identify boundary conditions that could potentially attenuate the occurrence of customer-directed sabotage. In this study, we explore potential attenuation effects of emotional stability and attentiveness on the customer injustice-sabotage linkage. The results showed emotional stability and attentiveness moderate the relationship between customer injustice and customer-directed sabotage. Specifically, the representatives with higher (vs. lower) emotional stability or higher (vs. lower) attentiveness are less likely to engage in customer-directed sabotage when they experience customer injustice. Moreover, there is a three-way interaction among daily customer injustice, emotional stability, and attentiveness that predicts daily customer-directed sabotage. Theoretical and practical contributions, limitations, and directions for future development are also discussed. | Song, Young Ho; Park, Jungkyu | Univ Windsor, Odette Sch Business, Windsor, ON, Canada; Kyungpook Natl Univ, Dept Psychol, Daegu, South Korea | 57221161189; 56206565000 | jkp@knu.ac.kr; | FRONTIERS IN PSYCHOLOGY | FRONT PSYCHOL | 1664-1078 | 13 | SSCI | PSYCHOLOGY, MULTIDISCIPLINARY | 2022 | 3.8 | 22.8 | 0.36 | 2025-06-25 | 4 | 3 | daily customer injustice; daily customer-directed sabotage; emotional stability; attentiveness; cross-level moderation effects | COUNTERPRODUCTIVE WORK BEHAVIOR; AFFECTIVE EVENTS THEORY; INDIVIDUAL-DIFFERENCES; CITIZENSHIP BEHAVIOR; SERVICE SABOTAGE; MORAL IDENTITY; MEDIATING ROLE; PERSONALITY; MISTREATMENT; AGGRESSION | attentiveness; cross-level moderation effects; daily customer injustice; daily customer-directed sabotage; emotional stability | English | 2022 | 2022-03-07 | 10.3389/fpsyg.2022.819396 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||||
| ○ | ○ | Article | Evolutionary analysis of Babesia vulpes and Babesia microti-like parasites | Background: The Babesia microti-like parasite is an emerging tick-borne piroplasm that has been detected in a range of hosts worldwide. Babesia vulpes, which is found in dogs and foxes, has been reclassified from B. microti-like parasites. The relationships among these B. microti-like parasites and B. vulpes with respect to host range and geographical origin have not been elucidated. Methods: Blood samples were collected from 27 raccoon dogs in South Korea and used to screen for B. microti-like parasites based on a PCR assay targeting the 18S rRNA gene of Babesia. For comparative purposes, in addition to 18S rRNA sequences from nine raccoon dogs, we also analyzed 18S rRNA sequences from B. microti-like parasites infecting hosts in different geographical regions worldwide obtained from the GenBank database, giving 123 sequences in total. The genetic variation and evolutionary relationships among these sequences were examined based on analyses using DnaSP, MEGA, Arlequine, and BEAST software. Results: Babesia microti-like parasites were identified in nine raccoon dogs and found to be related to B. vulpes obtained from Spanish dogs. Among the 123 sequences from 14 countries and various hosts, we identified 43 haplotypes with high genetic variance. Based on the genetic variance and phylogenetic analyses, we established that the B. microti-like parasites isolated in different geographical regions and from hosts belonging to five orders showed higher among-population variation than within-population variation. Babesia vulpes parasites infecting carnivore hosts, including raccoon dogs, foxes, skunks and dogs, appear to be genetically distinct from B. microti-like parasites infecting hosts belonging to the other orders. Conclusions: Our study demonstrated the genetic variation and evolutionary relationships among 18S rRNA sequences obtained from blood samples collected from various hosts and different geographical regions. Babesia vulpes was identified from raccoon dogs in South Korea. In addition, higher genetic variations were observed among populations of different hosts and geographical origins and, in particular, low connectivity was observed among host populations in the order Carnivora and those in other orders. These results suggest the B. vulpes, a piroplasmid species pathogenic in domestic dogs and wild canines, is genetically and evolutionarily different from B. microti-like parasites. | Lee, Sanghyun; Hong, Yeonchul; Chung, Dong-Il; Jang, Hyung-Kwan; Goo, Youn-Kyoung; Xuan, Xuenan | Korea Dis Control & Prevent Agcy, Dept Precis Med, Div Bio Bigdata, Korea Natl Inst Hlth, Cheongju, Chungbuk, South Korea; Kyungpook Natl Univ, Sch Med, Dept Parasitol & Trop Med, Daegu, South Korea; Jeonbuk Natl Univ, Coll Vet Med, Iksan, Jeonbuk, South Korea; Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido, Japan | 56272461500; 7403392847; 13310373300; 16689408200; 22937863200; 7005428448 | kuku1819@knu.ac.kr;gen@obihiro.ac.jp; | PARASITES & VECTORS | PARASITE VECTOR | 1756-3305 | 15 | 1 | SCIE | PARASITOLOGY;TROPICAL MEDICINE | 2022 | 3.2 | 22.9 | 0.99 | 2025-06-25 | 7 | 7 | Babesia vulpes; South Korea; Raccoon dogs; Babesia microti-like parasite; Evolutionary analysis | JAPAN; INFECTION; RODENT | Babesia microti-like parasite; Babesia vulpes; Evolutionary analysis; Raccoon dogs; South Korea | Animals; Babesia; Babesia microti; Babesiosis; Foxes; Parasites; Phylogeny; Raccoon Dogs; RNA, Ribosomal, 18S; RNA 18S; RNA 18S; Article; Babesia; Babesia microti; Babesia vulpes; biogeographic region; blood sampling; carnivore; controlled study; dog; evolution; fox; genetic variability; genetic variation; haplotype; host; nonhuman; nucleotide sequence; parasite isolation; phylogeny; polymerase chain reaction; raccoon dog; RNA gene; RNA sequence; skunk; South Korea; animal; genetics; parasite; parasitology; piroplasmosis | English | 2022 | 2022-11-03 | 10.1186/s13071-022-05528-9 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Oncologic outcomes according to the level of disease burden in patients with metachronous distant metastases from uterine cervical cancer: a Korean Radiation Oncology Group study (KROG 18-10) | Objective: This study aimed to evaluate the oncologic outcomes according to disease burden in uterine cervical cancer patients with metachronous distant metastases. Methods: Between 2005 and 2015, 163 patients with metachronous distant metastases from uterine cervical cancer after receiving a definitive therapy were evaluated at seven institutions in Korea. Low metastatic burden was defined as less than 5 metastatic sites, whereas high metastatic burden was others. Each metastasis site was divided based on the lymph node (LN) and organs affected. The overall survival (OS) and progression-free survival (PFS) were assessed. Cox proportional hazards models, including other clinical variables, were used to evaluate the survival outcomes. Results: The median follow-up duration was 22.2 months (range: 0.3-174.8 months). Para aortic LNs (56.4%), lungs (26.4%), supraclavicular LNs (18.4%), and peritoneum (13.5%) were found to be the common metastasis sites. Among 37 patients with a single metastasis, 17 (45.9%) had LN metastases and 20 (54.1%) had organ metastases. The 1-and 2-year OS rates were 73.9% and 55.0%, respectively, whereas the PFS rates were 67.2% and 42.9%, respectively. SCC Ag after recurrence and high metastatic burden were significant factors affecting the OS (p=0.004 and p<0.001, respectively). Distant organ recurrence, short disease-free interval (<= 2 years), and high metastatic burden were unfavorable factors for PFS (p=0.003, p=0.011, and p=0.002, respectively). Conclusion: A favorable oncologic outcome can be expected by performing salvage treatments in selected patients with a long disease-free interval, low metastatic burden, and/ or lymphatic-only metastasis. | Park, Sunmin; Park, Won; Park, Shin Hyung; Kim, Joo-Young; Kim, Jin Hee; Kim, Haeyoung; Kim, Yeon-Sil; Cho, Won Kyung; Yoon, Won Sup; Yang, Dae Sik | Korea Univ, Dept Radiat Oncol, Ansan Hosp, 123 Jeokgeum Ro, Ansan 15355, South Korea; Sungkyunkwan Univ, Samsung Med Ctr, Dept Radiat Oncol, Sch Med, Seoul, South Korea; Kyungpook Natl Univ, Sch Med, Dept Radiat Oncol, Daegu, South Korea; Natl Canc Ctr, Res Inst & Hosp, Dept Radiat Oncol, Goyang, South Korea; Keimyung Univ, Dongsan Med Ctr, Dept Radiat Oncol, Sch Med, Daegu, South Korea; Catholic Univ Korea, Dept Radiat Oncol, Seoul St Marys Hosp, Seoul, South Korea; Korea Univ Med Coll, Guro Hosp, Dept Radiat Oncol, Seoul, South Korea | ; Kim, Haeyoung/ABC-4815-2020; Kim, Hee/F-4594-2014; Park, Shinhyung/LNQ-6428-2024; Park, Soonchan/AAI-4682-2020 | 57042188100; 55663053400; 57203275843; 57207436986; 56441016600; 56007004100; 23019285600; 57201979532; 36629820700; 9233651800 | irionyws@korea.ac.kr;irionmphage@korea.ac.kr; | JOURNAL OF GYNECOLOGIC ONCOLOGY | J GYNECOL ONCOL | 2005-0380 | 2005-0399 | 33 | 3 | SCIE | OBSTETRICS & GYNECOLOGY;ONCOLOGY | 2022 | 3.9 | 22.9 | 0.14 | 2025-06-25 | 1 | 1 | Uterine Cervical Neoplasm; Recurrence; Metachronous Neoplasm; Neoplasm Metastasis; Lymphatic Metastasis | COLORECTAL-CARCINOMA METASTASES; HEPATIC RESECTION; RECURRENT; SURVIVAL; LIVER; SITE | Lymphatic Metastasis; Metachronous Neoplasm; Neoplasm Metastasis; Recurrence; Uterine Cervical Neoplasm | Cost of Illness; Female; Humans; Lymph Nodes; Lymphatic Metastasis; Neoplasm Recurrence, Local; Neoplasm Staging; Prognosis; Retrospective Studies; Uterine Cervical Neoplasms; antineoplastic agent; adult; aged; Article; cancer chemotherapy; cancer prognosis; cancer radiotherapy; cancer recurrence; cancer staging; cancer surgery; chemoradiotherapy; clinical decision making; clinical outcome; controlled study; disease burden; distant metastasis; female; follow up; human; Korea; lymph node metastasis; major clinical study; medical society; middle aged; overall survival; peritoneum metastasis; predictor variable; progression free survival; radiation oncology; second primary neoplasm; survival analysis; survival rate; treatment failure; uterine cervix cancer; very elderly; cost of illness; epidemiology; lymph node; pathology; prognosis; retrospective study; tumor recurrence; uterine cervix tumor | English | 2022 | 2022-05 | 10.3802/jgo.2022.33.e32 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Privacy-Aware Collaborative Task Offloading in Fog Computing | Numerous new applications have been proliferated with the mature of 5G, which generates a large number of latency-sensitive and computationally intensive mobile data requests. The real-time requirement of these mobile data has been accommodated well by fog computing in the past few years, mainly through offloading tasks to fog nodes in the vicinity. On the other hand, the user-privacy hidden in the Internet-of-Things (IoT) data has not been sufficiently considered in the presence of insecure fog nodes. It is risky to offload an entire mission-critical task to just one fog node or several fog nodes owned by the same service provider (SP), especially when the SP is marked with low-security credit and tends to collect data information of users for malicious use. To address this issue, we classify IoT user tasks based on their security requirements, divide them into different numbers of smaller fragments, and, finally, offload the segments of a task to multiple fog nodes owned by the same or various SPs according to their security requirements. The selected fog nodes will collaboratively serve the divided fragments to avoid the possible damage caused by the leak of sensitive data due to compromised fog nodes of malicious SPs. For this, we propose an integer linear programming (ILP) model and a dynamic programming algorithm to maximize the number of successfully served IoT data tasks with satisfactory security requirements while minimizing the end-to-end transmission delay. The numerical results show that the proposed ILP model and algorithm can significantly increase the successful provisioning ratio for tasks with high-security requirements. | Razaq, Mian Muaz; Tak, Byungchul; Peng, Limei; Guizani, Mohsen | Kyungpook Natl Univ, Sch Comp Sci & Engn, Daegu 41566, South Korea; Qatar Univ, Dept Comp Sci & Engn, Doha, Qatar | Guizani, Mohsen/AAX-4534-2021; Razaq, Muaz/ACN-8991-2022 | 57221661906; 6506911621; 7201574271; 7004750176 | mianmuaz97@gmail.com;bctak@knu.ac.kr;auroraplm@knu.ac.kr;mguizani@qu.edu.qa; | IEEE TRANSACTIONS ON COMPUTATIONAL SOCIAL SYSTEMS | IEEE T COMPUT SOC SY | 2329-924X | 9 | 1 | SCIE | COMPUTER SCIENCE, CYBERNETICS;COMPUTER SCIENCE, INFORMATION SYSTEMS | 2022 | 5 | 22.9 | 2.34 | 2025-06-25 | 18 | 22 | Task analysis; Security; Delays; Real-time systems; Edge computing; Cloud computing; Servers; Fog computing; Internet of Things (IoT); user privacy | Fog computing; Internet of Things (IoT); user privacy | 5G mobile communication systems; Cryptography; Dynamic programming; Fog; Integer programming; Internet of things; Privacy by design; Collaborative tasks; Dynamic programming algorithm; End-to-end transmission; Integer linear programming models; Internet of thing (IOT); Mission critical tasks; Real time requirement; Security requirements; Fog computing | English | 2022 | 2022-02 | 10.1109/tcss.2020.3047382 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | A Multivalent Vaccine Based on Ferritin Nanocage Elicits Potent Protective Immune Responses against SARS-CoV-2 Mutations | The SARS-CoV-2 pandemic has created a global public crisis and heavily affected personal lives, healthcare systems, and global economies. Virus variants are continuously emerging, and, thus, the pandemic has been ongoing for over two years. Vaccines were rapidly developed based on the original SARS-CoV-2 (Wuhan-Hu-1) to build immunity against the coronavirus disease. However, they had a very low effect on the virus' variants due to their low cross-reactivity. In this study, a multivalent SARS-CoV-2 vaccine was developed using ferritin nanocages, which display the spike protein from the Wuhan-Hu-1, B.1.351, or B.1.429 SARS-CoV-2 on their surfaces. We show that the mixture of three SARS-CoV-2 spike-protein-displaying nanocages elicits CD4(+) and CD8(+) T cells and B-cell immunity successfully in vivo. Furthermore, they generate a more consistent antibody response against the B.1.351 and B.1.429 variants than a monovalent vaccine. This leads us to believe that the proposed ferritin-nanocage-based multivalent vaccine platform will provide strong protection against emerging SARS-CoV-2 variants of concern (VOCs). | Kim, Seong A.; Kim, Seohyun; Kim, Gi Beom; Goo, Jiyoung; Kim, Nayeon; Lee, Yeram; Nam, Gi-Hoon; Lim, Seungho; Kim, Taeerk; Chang, Ki Hwan; Lee, Tae Gyu; Kim, In-San; Lee, Eun Jung | Korea Univ, KU KIST Grad Sch Converging Sci & Technol, Seoul 02841, South Korea; Korea Inst Sci & Technol, Biomed Res Inst, Ctr Theragnosis, Seoul 02456, South Korea; ShiftBio, Dept Res & Dev, Seoul 02751, South Korea; Kyung Hee Univ, KHU KIST Dept Converging Sci & Technol, Seoul 02447, South Korea; Kyungpook Natl Univ, Dept Chem Engn, Daegu 41566, South Korea; LabGen Corp, R&D Dept Drug Dev Div, Gyeonggi Do 13488, South Korea | Lee, Seung Eun/ABG-1607-2021; Nam, Gi-Hoon/I-6664-2018 | 57211273596; 57212447863; 57197865834; 57203060119; 59884547500; 57370627900; 57192919427; 57735386300; 57735415400; 57735355200; 57735386400; 34770432800; 57239965200 | 091989@kist.re.kr;kkksh03@shiftbio.net;gbkim@shiftbio.net;t16139@kist.re.kr;nykim0310@knu.ac.kr;yeram0301@knu.ac.kr;ghnam@shiftbio.net;lshcommy@gmail.com;tekim@labgenomics.com;jkhdw@labgenomics.com;tglee@labgenomics.com;iskim14@kist.re.kr;eunjunglee@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 11 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.97 | 2025-06-25 | 10 | 12 | protein nanocage; ferritin; COVID-19; SARS-CoV-2; variants; multivalent vaccine | SPIKE; NANOPARTICLES; INFECTIVITY; DESIGN | COVID-19; ferritin; multivalent vaccine; protein nanocage; SARS-CoV-2; variants | Antibodies, Neutralizing; CD8-Positive T-Lymphocytes; COVID-19; COVID-19 Vaccines; Ferritins; Humans; Immunity; Mutation; SARS-CoV-2; Vaccines, Combined; Viral Vaccines; ferritin; neutralizing antibody; vaccine; virus vaccine; CD8+ T lymphocyte; genetics; human; immunity; mutation; prevention and control | English | 2022 | 2022-06 | 10.3390/ijms23116123 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | A Novel RHS1 Locus in Rice Attributes Seed-Pod Shattering by the Regulation of Endogenous S-Nitrosothiols | Seed or pod shattering in rice (Oryza sativa) is considered to be one of the major factors involved in the domestication of rice as a crop. High seed shattering results in significant yield losses. In this study, we characterize the RICE HIGH SHATTERING 1 (RHS1) that corresponds to the locus LOCOₛ₀₄g₄₁₂₅₀ from a greenhouse screen, involving 145 Ac/Ds transposon mutant rice lines. The knockout mutant line rhs1 exhibited a significantly high shattering of grains in comparison to the wild-type plants. The exogenous application of nitric oxide (NO) resulted in a significant reduction in the expression of RHS1 in wild-type rice plants. The absence of RHS1, which encodes a putative armadillo/beta-catenin repeat family protein, resulted in high sensitivity of the rhs1 plants to nitrosative stress. Interestingly, the basal expression levels of QSH1 and SHAT1 genes (transcription factors that regulate seed-pod shattering in rice) were significantly lower in these plants than in wild-type plants; however, nitrosative stress negatively regulated the expression of QSH1 and SHAT1 in both WT and rhs1 plants, but positively regulated QSH4 expression in rhs1 plants alone. The expression levels of genes responsible for NO production (OsNIA1, OsNIA2, and OsNOA1) were lower in rhs1 plants than in WT plants under normal conditions. However, under nitrosative stress, the expression of OsNIA2 significantly increased in rhs1 plants. The expression of CPL1 (a negative regulator of seed shattering in rice) was significantly lower in rhs1 plants, and we found that CPL1 expression was correlated with S-nitrosothiol (SNO) alteration in rhs1. Interestingly noe1, a rice mutant with high SNO levels, exhibited low seed shattering, whereas rhs1 resulted in low SNO levels with high seed shattering. Therefore, RHS1 is a novel gene that negatively regulates the shattering trait in rice via regulation of endogenous SNO levels. However, the molecular mechanisms involved in the control of RHS1-mediated regulation of seed shattering and its interaction with nitric oxide and involvement in plant defense need to be investigated further. | Mun, Bong-Gyu; Shahid, Muhammad; Lee, Gang Sub; Hussain, Adil; Yun, Byung-Wook | Kyungpook Natl Univ, Coll Agr & Life Sci, Sch Appl Biosci, Daegu 41566, South Korea; Agr Res Inst, Khyber Pakhtunkhwa 19130, Mingora, Pakistan; Natl Inst Agr Sci, Biosafety Div, Jeonju 54875, South Korea; Abdul Wali Khan Univ, Dept Agr, Mardan 23200, Pakistan | Hussain, Dr. Adil/K-6016-2018; Mun, BongGyu/GYD-6010-2022; Hussain, Adil/K-6016-2018; Shahid, Muhammad/AAE-9424-2020 | 57147241300; 59577718600; 25927158200; 41961162600; 8245123600 | adilhussain@awkum.edu.pk;bwyun@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 21 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.27 | 2025-06-25 | 3 | 3 | seed shattering; rice; nitric oxide | BRITTLE RACHIS; TRANSCRIPTION FACTOR; GENETIC DISSECTION; RESPONSIVE GENES; REACTIVE OXYGEN; CULTIVATED RICE; STS MARKERS; DOMESTICATION; TRAITS; IDENTIFICATION | nitric oxide; rice; seed shattering | Nitric Oxide; Oryza; Quantitative Trait Loci; S-Nitrosothiols; Seeds; beta catenin; nitric oxide; s nitrosothiol; transcription factor; nitric oxide; s nitrosothiol; Article; gene expression level; gene locus; grain; greenhouse; knockout gene; nitrosative stress; nonhuman; plant defense; plant gene; plant seed; pod; qsh1 gene; rice; rice high shattering 1 gene; shat1 gene; transposon; wild plant; genetics; Oryza; plant seed; quantitative trait locus | English | 2022 | 2022-11 | 10.3390/ijms232113225 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Review | A Survey on Computational Methods for Investigation on ncRNA-Disease Association through the Mode of Action Perspective | Molecular and sequencing technologies have been successfully used in decoding biological mechanisms of various diseases. As revealed by many novel discoveries, the role of non-coding RNAs (ncRNAs) in understanding disease mechanisms is becoming increasingly important. Since ncRNAs primarily act as regulators of transcription, associating ncRNAs with diseases involves multiple inference steps. Leveraging the fast-accumulating high-throughput screening results, a number of computational models predicting ncRNA-disease associations have been developed. These tools suggest novel disease-related biomarkers or therapeutic targetable ncRNAs, contributing to the realization of precision medicine. In this survey, we first introduce the biological roles of different ncRNAs and summarize the databases containing ncRNA-disease associations. Then, we suggest a new trend in recent computational prediction of ncRNA-disease association, which is the mode of action (MoA) network perspective. This perspective includes integrating ncRNAs with mRNA, pathway and phenotype information. In the next section, we describe computational methodologies widely used in this research domain. Existing computational studies are then summarized in terms of their coverage of the MoA network. Lastly, we discuss the potential applications and future roles of the MoA network in terms of integrating biological mechanisms for ncRNA-disease associations. | Bang, Dongmin; Gu, Jeonghyeon; Park, Joonhyeong; Jeong, Dabin; Koo, Bonil; Yi, Jungseob; Shin, Jihye; Jung, Inuk; Kim, Sun; Lee, Sunho | Seoul Natl Univ, Interdisciplinary Program Bioinformat, Seoul 08826, South Korea; Seoul Natl Univ, Interdisciplinary Program Artificial Intelligence, Seoul 08826, South Korea; Seoul Natl Univ, Dept Comp Sci & Engn, Seoul 08826, South Korea; Kyungpook Natl Univ, Dept Comp Sci & Engn, Daegu 41566, South Korea; MOGAM Inst Biomed Res, Yongin 16924, South Korea; AIGENDRUG Co Ltd, Seoul 08826, South Korea | ; Jung, Inuk/T-1981-2018 | 57844148800; 57843640200; 57929079700; 57200327738; 57850731000; 57929079800; 57929646500; 56067575500; 36063436900; 57886967000 | sunholee@aigendrug.com; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 19 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.14 | 2025-06-25 | 4 | 4 | non-coding RNA; disease association; network mining; deep learning; mode of action; integrative analysis | DIFFERENTIAL EXPRESSION; CERNA NETWORK; DRUG RESPONSE; CIRCULAR RNAS; LNCRNA; DATABASE; GENE; MICRORNAS; MIRNA; PREDICTION | deep learning; disease association; integrative analysis; mode of action; network mining; non-coding RNA | Biomarkers; Computational Biology; RNA, Messenger; RNA, Untranslated; messenger RNA; untranslated RNA; biological marker; messenger RNA; untranslated RNA; bioinformatics; computer model; controlled study; deep learning; gene expression; high throughput screening; personalized medicine; phenotype; protein function; Review; biology; genetics; metabolism; procedures | English | 2022 | 2022-10 | 10.3390/ijms231911498 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | AMPK Amplifies IL2-STAT5 Signaling to Maintain Stability of Regulatory T Cells in Aged Mice | AMP-activated protein kinase (AMPK), an important regulator of the aging process, is expressed in various immune cells. However, its role in regulatory T cell (Treg) stability during aging is poorly understood. Here, we addressed the role of AMPK in Treg function and stability during aging by generating Treg-specific AMPK alpha 1 knockout mice. In this study, we found that AMPK alpha 1-deficient Tregs failed to control inflammation as effectively as normal Tregs did during aging. AMPK knockout from Tregs reduces STAT5 phosphorylation in response to interleukin (IL)-2 stimulation, thereby destabilizing Tregs by decreasing CD25 expression. Thus, our study addressed the role of AMPK in Tregs in sensing IL-2 signaling to amplify STAT5 phosphorylation, which, in turn, supports Treg stability by maintaining CD25 expression and controlling inflamm-aging. | Pokhrel, Ram Hari; Kang, Ben; Timilshina, Maheshwor; Chang, Jae-Hoon | Yeungnam Univ, Coll Pharm, Gyongsan 38541, South Korea; Kyungpook Natl Univ, Sch Med, Dept Pediat, Daegu 41944, South Korea | ; Timilshina, Maheshwor/AAW-1992-2021; 康, 奔/JMQ-0812-2023 | 57299414200; 57194823199; 57192978026; 24782066600 | jchang@yu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 23 | 20 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 1.06 | 2025-06-25 | 11 | 12 | aging; AMPK; inflammation; Treg stability | INFLAMMATION; METABOLISM; ACTIVATION | aging; AMPK; inflammation; Treg stability | AMP-Activated Protein Kinases; Animals; Forkhead Transcription Factors; Interleukin-2; Mice; Mice, Knockout; STAT5 Transcription Factor; T-Lymphocytes, Regulatory; hydroxymethylglutaryl coenzyme A reductase kinase; interleukin 2; interleukin 2 receptor alpha; STAT5 protein; forkhead transcription factor; hydroxymethylglutaryl coenzyme A reductase kinase; interleukin 2; STAT5 protein; aged; aging; animal cell; animal experiment; animal model; animal tissue; Article; controlled study; female; IL 2 signaling; inflammation; mouse; nonhuman; protein expression; protein phosphorylation; regulatory T lymphocyte; animal; genetics; knockout mouse; metabolism | English | 2022 | 2022-10 | 10.3390/ijms232012384 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Anti-Inflammatory Effect of Sparstolonin B through Inhibiting Expression of NF-κB and STAT-1 | Sparstolonin B (SsnB), which is found in Sparganium stoloniferum, prevents the synthesis of inflammatory mediators and is related to functional pathways of survival. In this study, we assessed the possible protective functions of SsnB on lipopolysaccharide (LPS)-induced inflammatory responses. We determined the functions of SsnB on controlling heme oxygenase (HO)-1, cyclooxygenase (COX-)2, and inducible nitric oxide synthase (iNOS) in LPS-activated human umbilical vein endothelial cells (HUVECs). Furthermore, the distinct function of SsnB on the expression of iNOS and well-known pro-inflammatory mediators, such as tumor necrosis factor (TNF)-alpha and interleukin (IL)-1 beta, were assessed in the pulmonary histological status of LPS-injected mice. SsnB upregulated the HO-1 production, inhibited luciferase-NF-kappa B interaction, and lowered COX-2/PGE2 and iNOS/NO, which lead to the reduction of STAT-1 phosphorylation. Moreover, SsnB enhanced the nuclear translocation of Nrf2, elevated the binding activity between Nrf2 and antioxidant response elements (AREs), and weakened IL-1 beta expression on LPS-treated HUVECs. SsnB-suppressed iNOS/NO synthesis was restored by the process of the RNAi inhibition of HO-1. In experiment with an LPS-injected animal model, SsnB remarkably decreased the iNOS expression in the pulmonary biostructure and TNF-alpha level in the bronchoalveolar lavage fluid (BALF). Therefore, these results demonstrate that SsnB is responsible for inflammation ameliorative activity by controlling iNOS through inhibition of both NF-kappa B expression and p-STAT-1. Therefore, SsnB could be a candidate for promoting novel clinical substances to remedy pathologic inflammation. | Kim, Nayeon; Kim, Chaeyeong; Ryu, Soo Ho; Kim, Go Oun; Bae, Jong-Sup | Kyungpook Natl Univ, Coll Pharm, Res Inst Pharmaceut Sci, Daegu 41566, South Korea | Bae, Jong-Sup/AAU-9724-2020 | 57226179942; 57418696700; 57418127000; 57896809800; 16021543200 | baejs@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 23 | 18 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2022 | 5.6 | 23.0 | 0.44 | 2025-06-25 | 5 | 5 | Sparstolonin B; endothelium; iNOS; p-STAT-1 | LIPOPOLYSACCHARIDE-INDUCED INFLAMMATION; SIGNALING PATHWAY; CARBON-MONOXIDE; ANTAGONIST; RELEASE; COX-2 | endothelium; iNOS; p-STAT-1; Sparstolonin B | Animals; Anti-Inflammatory Agents; Cyclooxygenase 2; Dinoprostone; Heme Oxygenase-1; Heterocyclic Compounds, 4 or More Rings; Human Umbilical Vein Endothelial Cells; Humans; Inflammation; Inflammation Mediators; Lipopolysaccharides; Luciferases; Mice; NF-E2-Related Factor 2; NF-kappa B; Nitric Oxide Synthase Type II; Tumor Necrosis Factor-alpha; antiinflammatory agent; cyclooxygenase 2; heme oxygenase 1; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; interleukin 1beta; luciferase; maslinic acid; nitrogen oxide; prostaglandin E2; sparstolonin B; STAT1 protein; transcription factor Nrf2; tumor necrosis factor; unclassified drug; antiinflammatory agent; autacoid; cyclooxygenase 2; fused heterocyclic rings; heme oxygenase 1; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; lipopolysaccharide; luciferase; prostaglandin E2; sparstolonin B; transcription factor Nrf2; tumor necrosis factor; animal experiment; animal model; animal tissue; antiinflammatory activity; antioxidant responsive element; Article; binding affinity; bronchoalveolar lavage fluid; controlled study; histopathology; human; human cell; in vivo study; lipopolysaccharide-induced inflammation; male; mouse; nonhuman; protein expression; protein phosphorylation; umbilical vein endothelial cell; upregulation; animal; genetics; inflammation; metabolism; pathology | English | 2022 | 2022-09 | 10.3390/ijms231810213 | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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