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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | ○ | Article | PF-3845, a Fatty Acid Amide Hydrolase Inhibitor, Directly Suppresses Osteoclastogenesis through ERK and NF-κB Pathways In Vitro and Alveolar Bone Loss In Vivo | Alveolar bone loss, the major feature of periodontitis, results from the activation of osteoclasts, which can consequently cause teeth to become loose and fall out; the development of drugs capable of suppressing excessive osteoclast differentiation and function is beneficial for periodontal disease patients. Given the difficulties associated with drug discovery, drug repurposing is an efficient approach for identifying alternative uses of commercially available compounds. Here, we examined the effects of PF-3845, a selective fatty acid amide hydrolase (FAAH) inhibitor, on receptor activator of nuclear factor kappa B ligand (RANKL)-mediated osteoclastogenesis, its function, and the therapeutic potential for the treatment of alveolar bone destruction in experimental periodontitis. PF-3845 significantly suppressed osteoclast differentiation and decreased the induction of nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) and the expression of osteoclast-specific markers. Actin ring formation and osteoclastic bone resorption were also reduced by PF-3845, and the anti-osteoclastogenic and anti-resorptive activities were mediated by the suppression of phosphorylation of rapidly accelerated fibrosarcoma (RAF), mitogen-activated protein kinase (MEK), extracellular signal-regulated kinase, (ERK) and nuclear factor kappa B (NF-kappa B) inhibitor (I kappa B alpha). Furthermore, the administration of PF-3845 decreased the number of osteoclasts and the amount of alveolar bone destruction caused by ligature placement in experimental periodontitis in vivo. The present study provides evidence that PF-3845 is able to suppress osteoclastogenesis and prevent alveolar bone loss, and may give new insights into its role as a treatment for osteoclast-related diseases. | Ihn, Hye-Jung; Kim, Yi-Seul; Lim, Soomin; Bae, Jong-Sup; Jung, Jae-Chang; Kim, Yeo-Hyang; Park, Jin-Woo; Wang, Zhao; Koh, Jeong-Tae; Bae, Yong-Chul; Baek, Moon-Chang; Park, Eui-Kyun | Kyungpook Natl Univ, Cell & Matrix Res Inst, Daegu 41944, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Oral Pathol & Regenerat Med, IHBR, Daegu 41940, South Korea; Kyungpook Natl Univ, Pharmaceut Sci Res Inst, Coll Pharm, CMRI, Daegu 41566, South Korea; Kyungpook Natl Univ, Dept Biol, Coll Nat Sci, Daegu 41566, South Korea; Kyungpook Natl Univ, Sch Med, Dept Pediat, Daegu 41944, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Periodontol, Daegu 41940, South Korea; Chonnam Natl Univ, Sch Dent, Dept Pharmacol & Dent Therapeut, Gwangju 61186, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Oral Anat & Neurobiol, Daegu 41940, South Korea; Kyungpook Natl Univ, Sch Med, Dept Mol Med, CMRI, Daegu 41944, South Korea | Bae, Jong-Sup/AAU-9724-2020 | 56421724400; 57221957771; 57202647759; 16021543200; 57203377467; 57032023800; 56815883000; 57221963455; 7201756951; 56377838800; 7006013097; 37071072400 | hjpihn@hanmail.net;ekdms121212@naver.com;friendship1240@hanmail.net;baejs@knu.ac.kr;jcjung@knu.ac.kr;kimhmd@knu.ac.kr;jinwoo@knu.ac.kr;htwz1996@163.com;jtkoh@chonnam.ac.kr;ycbae@knu.ac.kr;mcbaek@knu.ac.kr;epark@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 4 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.88 | 2025-07-30 | 11 | 13 | PF-3845; osteoclastogenesis; bone resorption; alveolar bone loss; ERK | Alveolar bone loss; Bone resorption; ERK; Osteoclastogenesis; PF-3845 | Alveolar Bone Loss; Amidohydrolases; Animals; Bone Resorption; Cells, Cultured; Disease Models, Animal; Macrophages; Male; MAP Kinase Signaling System; Mice; Mice, Inbred C57BL; NF-kappa B; Osteoclasts; Osteogenesis; Periodontitis; Piperidines; Pyridines; RANK Ligand; Treatment Outcome; bisphosphonic acid derivative; colony stimulating factor 1; cyclohexylcarbamic acid 3' carbamoylbiphenyl 3 yl ester; fatty acid amidase inhibitor; I kappa B kinase alpha; immunoglobulin enhancer binding protein; mitogen activated protein kinase; osteoclast differentiation factor; pf 3845; transcription factor NFAT; unclassified drug; amidase; fatty-acid amide hydrolase; immunoglobulin enhancer binding protein; osteoclast differentiation factor; PF 3845; piperidine derivative; pyridine derivative; Tnfsf11 protein, mouse; alveolar bone; alveolar bone loss; alveolar crest; animal cell; animal experiment; animal model; Article; bone destruction; bone slice; cementoenamel junction; controlled study; experimental periodontitis; fibrosarcoma; immunoblotting; immunofluorescence; in vitro study; in vivo study; intracellular signaling; male; mouse; nonhuman; osteoclast; osteoclastogenesis; osteolysis; protein expression; protein phosphorylation; treatment outcome; alveolar bone loss; animal; bone development; C57BL mouse; cell culture; disease model; drug effect; macrophage; MAPK signaling; metabolism; periodontitis | English | 2021 | 2021-02 | 10.3390/ijms22041915 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Review | Physiologically Active Molecules and Functional Properties of Soybeans in Human Health-A Current Perspective | In addition to providing nutrients, food can help prevent and treat certain diseases. In particular, research on soy products has increased dramatically following their emergence as functional foods capable of improving blood circulation and intestinal regulation. In addition to their nutritional value, soybeans contain specific phytochemical substances that promote health and are a source of dietary fiber, phospholipids, isoflavones (e.g., genistein and daidzein), phenolic acids, saponins, and phytic acid, while serving as a trypsin inhibitor. These individual substances have demonstrated effectiveness in preventing chronic diseases, such as arteriosclerosis, cardiac diseases, diabetes, and senile dementia, as well as in treating cancer and suppressing osteoporosis. Furthermore, soybean can affect fibrinolytic activity, control blood pressure, and improve lipid metabolism, while eliciting antimutagenic, anticarcinogenic, and antibacterial effects. In this review, rather than to improve on the established studies on the reported nutritional qualities of soybeans, we intend to examine the physiological activities of soybeans that have recently been studied and confirm their potential as a high-functional, well-being food. | Kim, Il-Sup; Kim, Cheorl-Ho; Yang, Woong-Suk | Kyungpook Natl Univ, Adv Bioresource Res Ctr, Daegu 41566, South Korea; Sungkyunkwan Univ, Dept Biol Sci, Mol & Cellular Glycobiol Unit, Gyunggi Do 16419, South Korea; Samsung Adv Inst Hlth Sci & Technol, Gyunggi Do 16419, South Korea; Nodaji Co Ltd, Pohang 37927, Gyeongsangbuk D, South Korea | Kim, Cheorl-Ho/T-6753-2019 | 55477678200; 7409877266; 57069270500 | 92kis@hanmail.net;chkimbio@skku.edu;yangws91@naver.com; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 8 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 2.25 | 2025-07-30 | 91 | 101 | soybean; active molecules; soy functionality; health benefit | CONJUGATED LINOLEIC-ACID; BOWMAN-BIRK INHIBITORS; SOY ISOFLAVONES; PHYTIC ACID; PHENOLIC-ACIDS; CHOLESTEROL CONTENT; BIOACTIVE PEPTIDES; LIPID-METABOLISM; DIETARY LIGNANS; GUT MICROBIOTA | Active molecules; Health benefit; Soy functionality; Soybean | Blood Circulation; Blood Pressure; Dietary Fiber; Fibrinolytic Agents; Humans; Intestines; Isoflavones; Lipid Metabolism; Phospholipids; Soybeans; alanine; arginine; aspartic acid; carbohydrate; glutamic acid; histidine; isoflavone derivative; isoleucine; leucine; lignan; linoleic acid; lysine; methionine; oligosaccharide; phenol derivative; phenylalanine; phosphatidylcholine; phytic acid; protein; saponin; threonine; tryptophan; tyrosine; valine; fibrinolytic agent; isoflavone derivative; phospholipid; chemical structure; dietary fiber; food composition; functional assessment; nonhuman; nutritional status; nutritional value; physiological process; Review; soybean; wellbeing; blood pressure; chemistry; circulation; dietary fiber; drug effect; human; intestine; lipid metabolism; soybean | English | 2021 | 2021-04 | 10.3390/ijms22084054 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Potential role of CMPK1, SLC29A1, and TLE4 polymorphisms in gemcitabine-based chemotherapy in HER2-negative metastatic breast cancer patients: pharmacogenetic study results from the prospective randomized phase II study of eribulin plus gemcitabine versus paclitaxel plus gemcitabine (KCSG-BR-13-11) | Background: In this study, we evaluated the association between genetic polymorphisms of 23 genes associated with gemcitabine metabolism and the clinical efficacy of gemcitabine in breast cancer patients. Patients and methods: This prospective, pharmacogenetic study was conducted in cooperation with a phase II clinical trial. A total of 103 genetic polymorphisms of the 23 genes involved in gemcitabine transport and metabolism were selected for genotyping. The associations of genetic polymorphisms with overall survival, progression-free survival (PFS), and 6-month PFS were analyzed. Results: A total of 91 breast cancer patients were enrolled in this study. In terms of 6-month PFS, rs1044457 in CMPK1 was the most significant genetic polymorphism [55.9% for CT and TT and 78.9% for CC, P < 0.001, hazard ratio (HR): 4.444, 95% confidence interval (CI): 1.905-10.363]. For the rs693955 in SLC29A1, the median duration of PFS was 5.4 months for AA and 10.5 months for CA and CC (P = 0.002, HR: 3.704, 95% CI: 1.615-8.497). For the rs2807312 in TLE4, the median duration of PFS was 5.7 months for TT and 10.4 months for CT and CC (P = 0.005, HR: 4.948, 95% CI: 1.612-15.190). In survival analysis with a multi-gene model, the TT genotype of rs2807312 had the worst PFS regardless of other genetic polymorphisms, whereas the CA genotype of rs693955 or the CT genotype of rs2807312 without the AA genotype of rs693955 had the best PFS compared with those of other genetic groups (P < 0.001). Conclusions: Genetic polymorphisms of rs1044457 in CMPK1, rs693955 in SLC29A1, and rs2807312 in TLE4 were significantly associated with the 6-month PFS rate and/or the duration of PFS. Further studies with a larger sample size and expression study would be helpful to validate the association of genetic polymorphisms and clinical efficacy of gemcitabine. | Cho, E. H.; Kim, J. -Y.; Im, S. -A.; Jung, K. H.; Sohn, J.; Lee, K. S.; Chae, Y. S.; Lee, K. H.; Kim, J. H.; Jang, J. -H.; Ahn, J. H.; Park, M. S.; Lee, S. -Y.; Park, Y. H. | Sungkyunkwan Univ, Sch Med, Kangbuk Samsung Hosp, Dept Lab Med, Seoul, South Korea; Sungkyunkwan Univ, Sch Med, Samsung Med Ctr, Div Hematol Oncol,Dept Med, 81 Irwon Ro, Seoul 06351, South Korea; Seoul Natl Univ, Dept Internal Med, Seoul Natl Univ Hosp, Canc Res Inst,Coll Med, Seoul, South Korea; Univ Ulsan, Coll Med, Asan Med Ctr, Dept Oncol, Seoul, South Korea; Yonsei Univ, Coll Med, Dept Internal Med, Div Med Oncol, Seoul, South Korea; Natl Canc Ctr, Ctr Breast Canc, Goyang, South Korea; Kyungpook Natl Univ Hosp, Dept Internal Med, Daegu, South Korea; Chungbuk Natl Univ Hosp, Dept Internal Med, Cheongju, South Korea; Seoul Natl Univ, Coll Med, Seoul Natl Univ Bundang Hosp, Dept Internal Med, Seoul, South Korea; Sungkyunkwan Univ, Sch Med, Samsung Med Ctr, Dept Lab Med & Genet, 81 Irwon Ro, Seoul 06351, South Korea; Samsung Med Ctr, Data Sci Res Inst, Biomed Stat Ctr, Seoul, South Korea; Keimyung Univ, Dept Stat, Daegu, South Korea; Samsung Med Ctr, Dept Clin Pharmacol & Therapeut, Seoul, South Korea; Sungkyunkwan Univ, Samsung Adv Inst Hlth Sci & Technol, Dept Hlth Sci & Technol, Seoul, South Korea; Korean Soc Med Oncol, Int Cooperat Comm, Seoul, South Korea | Im, Seock-Ah/J-5620-2012; LEE, SEOK-YONG/A-8012-2012; Kim, Ho-Young/HTR-7982-2023 | 57193661587; 58384966700; 34570185300; 15765006000; 15045889800; 17137276100; 57190793908; 55549466700; 56004266300; 35388364100; 59285952300; 57201652582; 7601413957; 35273175600 | suddenbz@skku.edu;yhparkhmo@skku.edu; | ESMO OPEN | ESMO OPEN | 2059-7029 | 6 | 5 | SCIE | ONCOLOGY | 2021 | 6.883 | 23.1 | 0.07 | 2025-07-30 | 2 | 2 | gemcitabine; breast cancer; genetic polymorphism; pharmacogenetics | EQUILIBRATIVE NUCLEOSIDE TRANSPORTER-1; GENETIC POLYMORPHISMS; 1ST-LINE CHEMOTHERAPY; DEOXYCYTIDINE KINASE; ADJUVANT GEMCITABINE; PREDICTS SURVIVAL; POLYMERASE-ETA; ASSOCIATION; SENSITIVITY; MULTICENTER | breast cancer; gemcitabine; genetic polymorphism; pharmacogenetics | Antineoplastic Combined Chemotherapy Protocols; Breast Neoplasms; Deoxycytidine; Equilibrative Nucleoside Transporter 1; Female; Furans; Humans; Ketones; Nuclear Proteins; Paclitaxel; Pharmacogenomic Testing; Polymorphism, Genetic; Prospective Studies; Repressor Proteins; cytidine monophosphate kinase 1; equilibrative nucleoside transporter 1; eribulin; gemcitabine; membrane protein; paclitaxel; protein tle4; unclassified drug; antineoplastic agent; deoxycytidine; equilibrative nucleoside transporter 1; eribulin; furan derivative; gemcitabine; ketone; nuclear protein; paclitaxel; repressor protein; SLC29A1 protein, human; TLE4 protein, human; adult; Article; cancer chemotherapy; cancer patient; cancer survival; combination drug therapy; comparative study; controlled study; DNA polymorphism; drug efficacy; female; genotype; genotyping; human; human epidermal growth factor receptor 2 negative breast cancer; major clinical study; metastatic breast cancer; overall survival; pharmacogenetic testing; phase 2 clinical trial; progression free survival; prospective study; randomized controlled trial; single nucleotide polymorphism; survival analysis; breast tumor; clinical trial; genetic polymorphism; genetics | English | 2021 | 2021-10 | 10.1016/j.esmoop.2021.100236 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Primary Ciliogenesis by 2-Isopropylmalic Acid Prevents PM2.5-Induced Inflammatory Response and MMP-1 Activation in Human Dermal Fibroblasts and a 3-D-Skin Model | Particulate matters (PMs) increase oxidative stress and inflammatory response in different tissues. PMs disrupt the formation of primary cilia in various skin cells, including keratinocytes and melanocytes. In this study, we found that 2-isopropylmalic acid (2-IPMA) promoted primary ciliogenesis and restored the PM2.5-induced dysgenesis of primary cilia in dermal fibroblasts. Moreover, 2-IPMA inhibited the generation of excessive reactive oxygen species and the activation of stress kinase in PM2.5-treated dermal fibroblasts. Further, 2-IPMA inhibited the production of pro-inflammatory cytokines, including IL-6 and TNF-alpha, which were upregulated by PM2.5. However, the inhibition of primary ciliogenesis by IFT88 depletion reversed the downregulated cytokines by 2-IPMA. Moreover, we found that PM2.5 treatment increased the MMP-1 expression in dermal fibroblasts and a human 3-D-skin model. The reduced MMP-1 expression by 2-IPMA was further reversed by IFT88 depletion in PM2.5-treated dermal fibroblasts. These findings suggest that 2-IPMA ameliorates PM2.5-induced inflammation by promoting primary ciliogenesis in dermal fibroblasts. | Bae, Ji-Eun; Min, Daejin; Choi, Ji Yeon; Choi, Hyunjung; Kim, Joon Bum; Park, Na Yeon; Jo, Doo Sin; Kim, Yong Hwan; Na, Hye-Won; Kim, Yoon Jae; Kim, Eun Sung; Kim, Hyoung-June; Cho, Dong-Hyung | Kyungpook Natl Univ, Brain Sci & Engn Inst, Daegu 41566, South Korea; AMOREPACIFIC Corp, R&D Ctr, Yongin 17074, South Korea; Kyungpook Natl Univ, Sch Life Sci, BK21 FOUR KNU Creat BioRes Grp, Daegu 41566, South Korea; Osong Med Innovat Fdn, New Drug Dev Ctr, Cheongju 28160, South Korea | Kim, Joon/ADP-8066-2022; Na, Hyewon/IVV-4674-2023; Choi, Hye Rin/JDV-9065-2023 | 57190605352; 57188550375; 57219085234; 50360927700; 57190611030; 57190609826; 56335489800; 57204676401; 57193759173; 57291039100; 57289815000; 35083721800; 35093684400 | loveg730@naver.com;djmin@amorepacific.com;muse41191@naver.com;heart@amorepacific.com;kss3213@naver.com;yeonie5613@gmail.com;doosinjo@gmail.com;yoo035913@gmail.com;serina@amorepacific.com;chodong02@hanmail.net;eskim@kbiohelth.kr;leojune@amorepacific.com;dhcho@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 20 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.59 | 2025-07-30 | 10 | 12 | 2-IPMA; primary cilia; dermal fibroblasts; oxidative stress; inflammation | IDENTIFICATION; INTERMEDIATE; YEAST; PM2.5; WINE | 2-IPMA; Dermal fibroblasts; Inflammation; Oxidative stress; Primary cilia | Cell Culture Techniques; Cell Line; Cilia; Cytokines; Enzyme Activation; Fibroblasts; Humans; Interleukin-6; JNK Mitogen-Activated Protein Kinases; Malates; Matrix Metalloproteinase 1; Models, Biological; Oxidative Stress; Particulate Matter; Reactive Oxygen Species; RNA Interference; RNA, Small Interfering; Tumor Necrosis Factor-alpha; Tumor Suppressor Proteins; Up-Regulation; 2 isopropylmalic acid; anthra[1,9 cd]pyrazol 6(2h) one; core protein; immunoglobulin enhancer binding protein; interleukin 6; interstitial collagenase; malic acid; phosphotransferase; reactive oxygen metabolite; skin protective agent; small interfering RNA; stress activated protein kinase; tumor necrosis factor; unclassified drug; 2-isopropylmalic acid; cytokine; IFT88 protein, human; interleukin 6; interstitial collagenase; malic acid derivative; reactive oxygen metabolite; stress activated protein kinase; tumor necrosis factor; tumor suppressor protein; antiinflammatory activity; Article; cell maturation; cell viability; ciliogenesis; controlled study; cytotoxicity; densitometry; down regulation; enzyme activation; enzyme activity; enzyme linked immunosorbent assay; fluorescence microscopy; human; human cell; inflammation; keratinocyte; melanocyte; oxidative stress; particulate matter 2.5; primary cilium; protein expression; skin cell; skin fibroblast; upregulation; biological model; cell culture technique; cell line; cilium; cytology; drug effect; fibroblast; genetics; metabolism; particulate matter; pathology; RNA interference; toxicity | English | 2021 | 2021-10 | 10.3390/ijms222010941 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Protective Effect of Ciclopirox against Ovariectomy-Induced Bone Loss in Mice by Suppressing Osteoclast Formation and Function | Postmenopausal osteoporosis is closely associated with excessive osteoclast formation and function, resulting in the loss of bone mass. Osteoclast-targeting agents have been developed to manage this disease. We examined the effects of ciclopirox on osteoclast differentiation and bone resorption in vitro and in vivo. Ciclopirox significantly inhibited osteoclast formation from primary murine bone marrow macrophages (BMMs) in response to receptor activator of nuclear factor kappa B ligand (RANKL), and the expression of genes associated with osteoclastogenesis and function was decreased. The formation of actin rings and resorption pits was suppressed by ciclopirox. Analysis of RANKL-mediated early signaling events in BMMs revealed that ciclopirox attenuates I kappa B alpha phosphorylation without affecting mitogen-activated protein kinase activation. Furthermore, the administration of ciclopirox suppressed osteoclast formation and bone loss in ovariectomy-induced osteoporosis in mice and reduced serum levels of osteocalcin and C-terminal telopeptide fragment of type I collagen C-terminus. These results indicate that ciclopirox exhibits antiosteoclastogenic activity both in vitro and in vivo and represents a new candidate compound for protection against osteoporosis and other osteoclast-related bone diseases. | Ihn, Hye Jung; Lim, Jiwon; Kim, Kiryeong; Nam, Sang-Hyeon; Lim, Soomin; Lee, Su Jeong; Bae, Jong-Sup; Kim, Tae Hoon; Kim, Jung-Eun; Baek, Moon-Chang; Bae, Yong Chul; Park, Eui Kyun | Kyungpook Natl Univ, Cell & Matrix Res Inst, Daegu 41944, South Korea; Kyungpook Natl Univ, Sch Dent, Inst Hard Tissue & Biotooth Regenerat IHBR, Dept Oral Pathol & Regenerat Med, Daegu 41940, South Korea; Kyungpook Natl Univ, Coll Pharm, Res Inst Pharmaceut Sci, Daegu 41566, South Korea; Daegu Univ, Dept Food Sci & Biotechnol, Gyongsan 38453, South Korea; Kyungpook Natl Univ, Sch Med, Dept Mol Med, Daegu 41944, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Oral Anat & Neurobiol, Daegu 41940, South Korea | ; Lee, Su-Jeong/AAH-8467-2021; Bae, Jong-Sup/AAU-9724-2020 | 56421724400; 13404844200; 57200729845; 57209806061; 57202647759; 57219236074; 16021543200; 56906394500; 57209054588; 7006013097; 56377838800; 37071072400 | hjpihn@hanmail.net;jiwonparadise@hanmail.net;kileyong93@daum.net;aay0805@naver.com;friendship1240@hanmail.net;marhaul@hanmail.net;baejs@knu.ac.kr;skyey7@daegu.ac.kr;kjeun@knu.ac.kr;mcbaek@knu.ac.kr;ycbae@knu.ac.kr;epark@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 15 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.29 | 2025-07-30 | 5 | 4 | osteoclast; bone resorption; ciclopirox; osteoporosis | NF-KAPPA-B; RECEPTOR ACTIVATOR; IN-VITRO; DIFFERENTIATION; MECHANISMS; OLAMINE; FUSION; RANK | Bone resorption; Ciclopirox; Osteoclast; Osteoporosis | Animals; Antifungal Agents; Bone Resorption; Cell Differentiation; Cells, Cultured; Ciclopirox; Female; Male; Mice; Mice, Inbred C57BL; Osteoclasts; Osteogenesis; Ovariectomy; Protective Agents; RANK Ligand; antifungal agent; ciclopirox; osteoclast differentiation factor; protective agent; Tnfsf11 protein, mouse; adverse event; animal; bone development; C57BL mouse; cell culture; cell differentiation; cytology; drug effect; drug therapy; etiology; female; genetics; male; metabolism; mouse; osteoclast; osteolysis; ovariectomy; pathology | English | 2021 | 2021-08 | 10.3390/ijms22158299 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Review | Protein-Based Nanoparticle Vaccines for SARS-CoV-2 | The pandemic caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has upended healthcare systems and economies around the world. Rapid understanding of the structural biology and pathogenesis of SARS-CoV-2 has allowed the development of emergency use or FDA-approved vaccines and various candidate vaccines. Among the recently developed SARS-CoV-2 candidate vaccines, natural protein-based nanoparticles well suited for multivalent antigen presentation and enhanced immune stimulation to elicit potent humoral and cellular immune responses are currently being investigated. This mini-review presents recent innovations in protein-based nanoparticle vaccines against SARS-CoV-2. The design and strategy of displaying antigenic domains, including spike protein, receptor-binding domain (RBD), and other domains on the surface of various protein-based nanoparticles and the performance of the developed nanoparticle-based vaccines are highlighted. In the final part of this review, we summarize and discuss recent advances in clinical trials and provide an outlook on protein-based nanoparticle vaccines. | Sung, Hyo-Dong; Kim, Nayeon; Lee, Yeram; Lee, Eun Jung | Kyungpook Natl Univ, Sch Appl Chem Engn, Dept Chem Engn, Daegu 41566, South Korea | Lee, Seung Eun/ABG-1607-2021 | 57222632058; 59884547500; 57370627900; 57239965200 | gyehd0314@knu.ac.kr;nykim0310@knu.ac.kr;yeram0301@knu.ac.kr;eunjunglee@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 22 | 24 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.49 | 2025-07-30 | 18 | 19 | SARS-CoV-2; protein nanoparticles; vaccines; nanovaccine; nanomedicine; protein-based nanotechnology | NEUTRALIZING ANTIBODY-RESPONSES; RATIONAL DESIGN; FERRITIN; SPIKE; NANOCAGES; RBD | Nanomedicine; Nanovaccine; Protein nanoparticles; Protein-based nanotechnology; SARS-CoV-2; Vaccines | Animals; Antibodies, Neutralizing; Antibodies, Viral; Antigen Presentation; COVID-19; COVID-19 Vaccines; Humans; Nanoparticles; SARS-CoV-2; Spike Glycoprotein, Coronavirus; Vaccines; nanoparticle; protein vaccine; SARS-CoV-2 vaccine; virus spike protein; coronavirus spike glycoprotein; nanoparticle; neutralizing antibody; spike protein, SARS-CoV-2; vaccine; virus antibody; antigen presentation; cellular immunity; clinical trial (topic); hamster; human; humoral immunity; immunostimulation; Leporidae; Macaca; Mustela putorius furo; nonhuman; protein domain; receptor binding; Review; Severe acute respiratory syndrome coronavirus 2; animal; chemistry; immunology; pathogenicity; pharmacology | English | 2021 | 2021-12 | 10.3390/ijms222413445 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | PRP4 Promotes Skin Cancer by Inhibiting Production of Melanin, Blocking Influx of Extracellular Calcium, and Remodeling Cell Actin Cytoskeleton | Pre-mRNA processing factor 4B (PRP4) has previously been shown to induce epithelial-mesenchymal transition (EMT) and drug resistance in cancer cell lines. As melanin plays an important photoprotective role in the risk of sun-induced skin cancers, we have investigated whether PRP4 can induce drug resistance and regulate melanin biosynthesis in a murine melanoma (B16F10) cell line. Cells were incubated with a crucial melanogenesis stimulator, alpha-melanocyte-stimulating hormone, followed by transfection with PRP4. This resulted in the inhibition of the production of melanin via the downregulation of adenylyl cyclase-cyclic adenosine 3 ',5 '-monophosphate (AC)-(cAMP)-tyrosinase synthesis signaling pathway. Inhibition of melanin production by PRP4 leads to the promotion of carcinogenesis and induced drug resistance in B16F10 cells. Additionally, PRP4 overexpression upregulated the expression of beta-arrestin 1 and desensitized the extracellular calcium-sensing receptor (CaSR), which in turn, inhibited the influx of extracellular Ca2+ ions. The decreased influx of Ca2+ was confirmed by a decreased expression level of calmodulin. We have demonstrated that transient receptor potential cation channel subfamily C member 1 was involved in the influx of CaSR-induced Ca2+ via a decreasing level of its expression. Furthermore, PRP4 overexpression downregulated the expression of AC, decreased the synthesis of cAMP, and modulated the actin cytoskeleton by inhibiting the expression of Ras homolog family member A (RhoA). Our investigation suggests that PRP4 inhibits the production of melanin in B16F10 cells, blocks the influx of Ca2+ through desensitization of CaSR, and modulates the actin cytoskeleton through downregulating the AC-cAMP pathway; taken together, these observations collectively lead to the promotion of skin carcinogenesis. | Ahmed, Muhammad Bilal; Ul Islam, Salman; Lee, Young Sup | Kyungpook Natl Univ, Sch Life Sci, BK21 FOUR KNU Creat Biores Grp, Daegu 41566, South Korea | 58689879600; 56985186700; 36013628200 | muhammad786@knu.ac.kr;salman2013@knu.ac.kr;yselee@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 13 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.52 | 2025-07-30 | 9 | 9 | PRP4; melanocyte; cAMP; calcium-sensing receptor; actin cytoskeleton; drug resistance | PROTEIN-KINASE-A; SENSING RECEPTOR; CYCLIC-AMP; TYROSINASE; MIGRATION; CHANNELS; GENE; CAMP; PHOSPHORYLATION; MELANOGENESIS | Actin cytoskeleton; Calcium-sensing receptor; CAMP; Drug resistance; Melanocyte; PRP4 | Actin Cytoskeleton; Animals; Antineoplastic Agents; Calcium; Calmodulin; Carrier Proteins; Cell Line, Tumor; Drug Resistance, Neoplasm; Extracellular Space; Gene Expression Regulation, Neoplastic; Humans; Intracellular Space; Melanins; Melanoma, Experimental; Mice; Protein Binding; Ribonucleoprotein, U4-U6 Small Nuclear; Signal Transduction; Skin Neoplasms; adenylate cyclase; alpha intermedin; beta arrestin 1; calmodulin; cyclic AMP; F actin; melanin; melanocortin 1 receptor; messenger RNA precursor processing factor 4b; microphthalmia associated transcription factor; monophenol monooxygenase; protein; RhoA guanine nucleotide binding protein; small interfering RNA; transient receptor potential channel; tyrosine; unclassified drug; antineoplastic agent; calcium; calmodulin; carrier protein; melanin; protein binding; PRPF4 protein, human; small nuclear ribonucleoprotein; actin filament; animal cell; animal experiment; animal model; Article; B16-BL6 cell line; calcium cell level; cAMP assay; controlled study; desensitization; down regulation; enzyme activation; extracellular calcium; fluorescence intensity; gene knockdown; genetic transcription; genetic transfection; in vivo study; male; melanogenesis; melanoma; mouse; nonhuman; protein expression; protein function; real time polymerase chain reaction; reverse transcription polymerase chain reaction; signal transduction; skin carcinogenesis; transient transfection; tumor growth; tumor volume; upregulation; Western blotting; actin filament; animal; biosynthesis; drug effect; drug resistance; experimental melanoma; extracellular space; gene expression regulation; genetics; human; intracellular space; metabolism; pathology; skin tumor; tumor cell line | English | 2021 | 2021-07 | 10.3390/ijms22136992 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Review | Regulation of Gene Expression by Telomere Position Effect | Many diseases that involve malignant tumors in the elderly affect the quality of human life; therefore, the relationship between aging and pathogenesis in geriatric diseases must be under-stood to develop appropriate treatments for these diseases. Recent reports have shown that epigenetic regulation caused by changes in the local chromatin structure plays an essential role in aging. This review provides an overview of the roles of telomere shortening on genomic structural changes during an age-dependent shift in gene expression. Telomere shortening is one of the most prominent events that is involved in cellular aging and it affects global gene expression through genome rearrangement. This review provides novel insights into the roles of telomere shortening in disease-affected cells during pathogenesis and suggests novel therapeutic approaches. | Lee, Kyung-Ha; Kim, Do-Yeon; Kim, Wanil | Daegu Haany Univ, Div Cosmet Sci & Technol, Gyongsan 38610, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Pharmacol, Daegu 41940, South Korea; Gyeongsang Natl Univ, Inst Hlth Sci, Dept Convergence Med Sci, Dept Biochem,Sch Med, Jinju 52727, South Korea | Kim, Wanil/I-7849-2015; Kim, Do-Yeon/AET-3021-2022; Lee, Kyung-Ha/GRY-2640-2022 | 54967786000; 57203012542; 7405813437 | kyungha.lee@dhu.ac.kr;dykim82@knu.ac.kr;wkim@gnu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 22 | 23 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.29 | 2025-07-30 | 13 | 12 | telomere; aging; gene expression; telomere position effect; telomere looping | TERT PROMOTER MUTATIONS; FACIOSCAPULOHUMERAL MUSCULAR-DYSTROPHY; CELL BIOLOGY; IN-VIVO; DNA-DAMAGE; LENGTH; CHROMATIN; HETEROCHROMATIN; SENESCENCE; SHELTERIN | Aging; Gene expression; Telomere; Telomere looping; Telomere position effect | Aging; Animals; Epigenesis, Genetic; Gene Expression Regulation; Humans; Neoplasms; Telomere; cell aging; gene expression regulation; genome; human; nonhuman; pathogenesis; position effect; Review; telomere shortening; aging; animal; chemistry; genetic epigenesis; genetics; neoplasm; pathology; telomere | English | 2021 | 2021-12 | 10.3390/ijms222312807 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Review | RNA-Binding Proteins and the Complex Pathophysiology of ALS | Genetic analyses of patients with amyotrophic lateral sclerosis (ALS) have identified disease-causing mutations and accelerated the unveiling of complex molecular pathogenic mechanisms, which may be important for understanding the disease and developing therapeutic strategies. Many disease-related genes encode RNA-binding proteins, and most of the disease-causing RNA or proteins encoded by these genes form aggregates and disrupt cellular function related to RNA metabolism. Disease-related RNA or proteins interact or sequester other RNA-binding proteins. Eventually, many disease-causing mutations lead to the dysregulation of nucleocytoplasmic shuttling, the dysfunction of stress granules, and the altered dynamic function of the nucleolus as well as other membrane-less organelles. As RNA-binding proteins are usually components of several RNA-binding protein complexes that have other roles, the dysregulation of RNA-binding proteins tends to cause diverse forms of cellular dysfunction. Therefore, understanding the role of RNA-binding proteins will help elucidate the complex pathophysiology of ALS. Here, we summarize the current knowledge regarding the function of disease-associated RNA-binding proteins and their role in the dysfunction of membrane-less organelles. | Kim, Wanil; Kim, Do-Yeon; Lee, Kyung-Ha | Daegu Haany Univ, Div Cosmet Sci & Technol, Hanuidae Ro 1, Gyongsan 38610, Gyeongbuk, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Pharmacol, Daegu 41940, South Korea; Gyeongsang Natl Univ, Coll Med, Dept Biochem, Jinju 52828, South Korea | Lee, Kyung-Ha/GRY-2640-2022; Kim, Do-Yeon/AET-3021-2022 | 7405813437; 57203012542; 54967786000 | wkim@dhu.ac.kr;dykim82@knu.ac.kr;kyungha.lee@dhu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 5 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.35 | 2025-07-30 | 12 | 14 | ALS; RNA-binding protein; membrane-less organelles | REPEAT EXPANSION; HEXANUCLEOTIDE REPEAT; C9ORF72; MUTATIONS; GRANULES; TRANSLATION; MECHANISMS; TOXICITY; BODIES | ALS; Membrane-less organelles; RNA-binding protein | Amyotrophic Lateral Sclerosis; Humans; RNA; RNA-Binding Proteins; RNA binding protein; RNA; RNA binding protein; amyotrophic lateral sclerosis; cell disruption; cell granule; cell inclusion; cell organelle; coiled body; gene expression; gene mutation; human; nucleocytoplasmic transport; nucleolus; pathophysiology; Review; RNA splicing; amyotrophic lateral sclerosis; metabolism | English | 2021 | 2021-03 | 10.3390/ijms22052598 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | S100 Calcium-Binding Protein P Secreted from Megakaryocytes Promotes Osteoclast Maturation | Megakaryocytes (MKs) differentiate from hematopoietic stem cells and produce platelets at the final stage of differentiation. MKs directly interact with bone cells during bone remodeling. However, whether MKs are involved in regulating bone metabolism through indirect regulatory effects on bone cells is unclear. Here, we observed increased osteoclast differentiation of bone marrow-derived macrophages (BMMs) cultured in MK-cultured conditioned medium (MK CM), suggesting that this medium contains factors secreted from MKs that affect osteoclastogenesis. To identify the MK-secreted factor, DNA microarray analysis of the human leukemia cell line K562 and MKs was performed, and S100 calcium-binding protein P (S100P) was selected as a candidate gene affecting osteoclast differentiation. S100P was more highly expressed in MKs than in K562 cells, and showed higher levels in MK CM than in K562-cultured conditioned medium. In BMMs cultured in the presence of recombinant human S100P protein, osteoclast differentiation was promoted and marker gene expression was increased. The resorption area was significantly larger in S100P protein-treated osteoclasts, demonstrating enhanced resorption activity. Overall, S100P secreted from MKs promotes osteoclast differentiation and resorption activity, suggesting that MKs indirectly regulate osteoclast differentiation and activity through the paracrine action of S100P. | Lee, Seung-Hoon; Ihn, Hye Jung; Park, Eui Kyun; Kim, Jung-Eun | Kyungpook Natl Univ, Sch Med, Dept Mol Med, Daegu 41944, South Korea; Kyungpook Natl Univ, Dept Biomed Sci, BK21 KNU Convergence Educ Program Biomed Sci Crea, Daegu 41944, South Korea; Kyungpook Natl Univ, Cell & Matrix Res Inst, Daegu 41944, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Oral Pathol & Regenerat Med, IHBR, Daegu 41944, South Korea | 59056027600; 56421724400; 37071072400; 57209054588 | jsat1234@naver.com;hjpihn@hanmail.net;epark@knu.ac.kr;kjeun@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 11 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.59 | 2025-07-30 | 9 | 8 | S100P; megakaryocyte; osteoclastogenesis | NF-KAPPA-B; BONE-MARROW; CA2+-BINDING PROTEIN; DIFFERENTIATION; PROLIFERATION; EXPRESSION; RESORPTION; SURVIVAL; RECEPTOR; CANCER | Megakaryocyte; Osteoclastogenesis; S100P | Calcium-Binding Proteins; Cell Differentiation; Cells, Cultured; Humans; K562 Cells; Megakaryocytes; Neoplasm Proteins; Osteoclasts; Osteogenesis; calcium binding protein; S100 calcium binding protein P; unclassified drug; calcium binding protein; S100P protein, human; tumor protein; Article; bone marrow derived macrophage; cell differentiation; cell maturation; DNA microarray; gene expression; human; human cell; K-562 cell line; marker gene; megakaryocyte; osteoclast; osteoclastogenesis; paracrine signaling; bone development; cell culture; cell differentiation; cytology; genetics; megakaryocyte; metabolism; osteoclast | English | 2021 | 2021-06 | 10.3390/ijms22116129 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Senescence Marker Protein 30 (SMP30): A Novel Pan-Species Diagnostic Marker for the Histopathological Diagnosis of Breast Cancer in Humans and Animals | Senescence marker protein 30 (SMP30) is a cell survival factor playing an important role in vitamin C synthesis and antiapoptosis. Moreover, its cytoprotective role suggests a possibility to be related to cancer cell survival. Mammary carcinoma is a common cancer in both humans and animals. Because of its histopathological diversity, especially in the early stage, histopathological diagnosis may be complicated; therefore, a diagnostic marker is helpful for confirmation. The present study analyzed the expression pattern of SMP30 in mammary carcinoma in humans, dogs, and cats. Immunohistochemistry, immunofluorescence, and western blot analysis were used to investigate SMP30 expression patterns. The expression was specifically observed in neoplastic glandular epithelial cells. The expression increased with the malignancy of glandular epithelial cells with a highly proliferative status. However, SMP30 expression was low in normal mammary gland tissues or well-differentiated adenoma tissues. The patterns were consistently reproduced in canine primary mammary carcinoma cells and MCF-7 and MDA-MB-231 human carcinoma cell lines. This study provides useful information to understand SMP30 expression in various stages of mammary carcinoma and to suggest its utility as a pan-species diagnostic marker, thereby helping to establish strategies for diagnosing mammary carcinoma in several species. | Baek, Su-Min; Lee, Seoung-Woo; Kim, Tae-Un; Choi, Seong-Kyoon; Yun, Sungho; Lee, Won-Jae; Han, Se-Hyeon; Hong, Il-Hwa; Park, Sang-Joon; Kim, Tae-Hwan; Jeong, Kyu-Shik; Park, Jin-Kyu | Kyungpook Natl Univ, Dept Vet Pathol, Coll Vet Med, Daegu 41566, South Korea; Daegu Gyeongbuk Inst Sci & Technol DGIST, Core Prot Resources Ctr, Daegu 42988, South Korea; Kyungpook Natl Univ, Coll Vet Med, Daegu 41566, South Korea; Seoul Broadcasting Stn SBS, Dept News Team, Seoul 07574, South Korea; Gyeongsang Natl Univ, Dept Vet Pathol, Coll Vet Med, Jinju 52828, South Korea; Kyungpook Natl Univ, Stem Cell Therapeut Res Inst, Daegu 41566, South Korea | 57207938426; 57218827581; 57214091908; 55505432500; 57201366734; 57205486455; 57210915063; 8702273100; 7501825941; 57202984578; 7201556460; 35213723500 | suminbaek@naver.com;pyrk2000@gmail.com;tukim92@naver.com;cskbest@dgist.ac.kr;shyun@knu.ac.kr;iamcyshd@knu.ac.kr;vetman@sbs.co.kr;ihhong@gnu.ac.kr;psj26@knu.ac.kr;thkim56@knu.ac.kr;jeongks@knu.ac.kr;jinkyu820@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 22 | 5 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.44 | 2025-07-30 | 6 | 7 | breast cancer; cat; diagnostic marker; dog; human; mammary carcinoma; neoplastic glandular epithelial cell; SMP30 | CANINE MAMMARY-TUMORS; CLINICOPATHOLOGICAL FEATURES; PROGESTERONE-RECEPTORS; DEFICIENCY INCREASES; YOUNG-WOMEN; APOPTOSIS; REGUCALCIN; STRESS; AGE; ROS | Breast cancer; Cat; Diagnostic marker; Dog; Human; Mammary carcinoma; Neoplastic glandular epithelial cell; SMP30 | Animals; Biomarkers, Tumor; Breast; Breast Neoplasms; Calcium-Binding Proteins; Cat Diseases; Cats; Cell Line, Tumor; Dog Diseases; Dogs; Female; Humans; Intracellular Signaling Peptides and Proteins; Mammary Neoplasms, Animal; MCF-7 Cells; Prognosis; ascorbic acid; cytokeratin; regucalcin; tumor marker; calcium binding protein; RGN protein, human; signal peptide; tumor marker; Article; breast cancer; breast carcinoma; cancer grading; cancer localization; carcinoma cell; cat; cell proliferation; cell survival; correlation analysis; diagnostic value; dog; epithelium cell; histopathology; human; human cell; human tissue; immunofluorescence; immunohistochemistry; mammary gland; MCF-7 cell line; MDA-MB-231 cell line; nonhuman; protein expression; Western blotting; animal; breast; breast tumor; cat disease; dog disease; experimental mammary neoplasm; female; pathology; prognosis; tumor cell line | English | 2021 | 2021-03 | 10.3390/ijms22052340 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Serum-Derived Neuronal Exosomal miRNAs as Biomarkers of Acute Severe Stress | Stress is the physical and psychological tension felt by an individual while adapting to difficult situations. Stress is known to alter the expression of stress hormones and cause neuroinflammation in the brain. In this study, miRNAs in serum-derived neuronal exosomes (nEVs) were analyzed to determine whether differentially expressed miRNAs could be used as biomarkers of acute stress. Specifically, acute severe stress was induced in Sprague-Dawley rats via electric foot-shock treatment. In this acute severe-stress model, time-dependent changes in the expression levels of stress hormones and neuroinflammation-related markers were analyzed. In addition, nEVs were isolated from the serum of control mice and stressed mice at various time points to determine when brain damage was most prominent; this was found to be 7 days after foot shock. Next-generation sequencing was performed to compare neuronal exosomal miRNA at day 7 with the neuronal exosomal miRNA of the control group. From this analysis, 13 upregulated and 11 downregulated miRNAs were detected. These results show that specific miRNAs are differentially expressed in nEVs from an acute severe-stress animal model. Thus, this study provides novel insights into potential stress-related biomarkers. | Sung, Minkyoung; Sung, Soo-Eun; Kang, Kyung-Ku; Choi, Joo-Hee; Lee, Sijoon; Kim, KilSoo; Lim, Ju-Hyeon; Lee, Gun Woo; Rim, Hyo-Deog; Kim, Byung-Soo; Won, Seunghee; Kim, Kyungmin; Jang, Seoyoung; Seo, Min-Soo; Woo, Jungmin | Daegu Gyeongbuk Med Innovat Fdn DGMIF, Dept Lab, Anim Ctr, Daegu 41061, South Korea; Kyungpook Natl Univ, Sch Med, Dept Psychiat, Daegu 41944, South Korea; Kyungpook Natl Univ, Coll Vet Med, Dept Vet Toxicol, 80 Daehakro, Daegu 41566, South Korea; Osong Med Innovat Fdn, New Drug Dev Ctr, Chungbuk 28160, South Korea; Yeungnam Univ, Med Ctr, Dept Orthoped Surg, Coll Med, 170 Hyonchung Ro, Daegu 42415, South Korea | ; Kim, Byung-Soo/H-4047-2013; Lee, Sijoon/LIC-1291-2024 | 57256838000; 56051194000; 57215079376; 55882556800; 57219164019; 35272034300; 57218821150; 55599978600; 15768078500; 57214661242; 35278955600; 57201480192; 57258167000; 35254332100; 56241307600 | tjdalsrud27@naver.com;sesung@dgmif.re.kr;kangkk@dgmif.re.kr;cjh522@dgmif.re.kr;sjlee1013@dgmif.re.kr;kskim728@knu.ac.kr;globaljh2019@gmail.com;gwlee1871@gmail.com;hdrim@mail.knu.ac.kr;because99@hanmail.net;wonsh864@knu.ac.kr;for-bluewish@hanmail.net;seoyoung870314@daum.net;msseo@dgmif.re.kr;woojm3@hanmail.net; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 18 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.44 | 2025-07-30 | 7 | 7 | acute severe stress; neuroinflammation; exosomes; miRNA; biomarkers | CHRONIC PSYCHOSOCIAL STRESS; FOOT-SHOCK STRESS; EXTRACELLULAR VESICLES; POTENTIAL BIOMARKERS; CELL-PROLIFERATION; BDNF EXPRESSION; STEM-CELLS; RAT; MICRORNAS; RESPONSES | Acute severe stress; Biomarkers; Exosomes; MiRNA; Neuroinflammation | Acute Disease; Animals; Biomarkers; Exosomes; Gene Ontology; Hormones; Hypothalamo-Hypophyseal System; Inflammation; Male; MicroRNAs; Neurons; Rats, Sprague-Dawley; Stress, Psychological; beta tubulin; brain derived neurotrophic factor; corticosterone; cyclooxygenase 2; glial fibrillary acidic protein; hydrocortisone; microRNA; microRNA 126a 5p; microRNA 140 3p; microRNA 17 5p; microRNA 191a 5p; microRNA 19b 3p; microRNA 24 3p; microRNA 30c 5p; microRNA 3473; microRNA 425 5p; microRNA 466b 3p; microRNA 93 5p; microRNA 98 3p; microRNA let 7a 1 3p; microRNA let 7a 5p; microRNA let 7b 3p; microRNA let 7c 2 3p; microRNA let 7c 5p; microRNA let 7d 3p; microRNA let 7d 5p; microRNA let 7f 1 3p; microRNA let 7f 5p; microRNA let 7g 5p; microRNA let 7i 5p; neuron specific class iii beta tubulin; neuron specific enolase; stress hormone; unclassified drug; biological marker; hormone; microRNA; acute severe stress; acute stress; animal cell; animal experiment; animal model; animal tissue; Article; bilayer membrane; brain damage; cell isolation; controlled study; corticosterone blood level; down regulation; enzyme linked immunosorbent assay; exosome; flow cytometry; footshock; high throughput sequencing; hippocampus; hydrocortisone blood level; KEGG; nervous system inflammation; nonhuman; prediction; protein expression level; rat; RNA analysis; signal transduction; transmission electron microscopy; upregulation; acute disease; animal; blood; exosome; gene ontology; genetics; hypothalamus hypophysis system; inflammation; male; mental stress; metabolism; nerve cell; pathology; Sprague Dawley rat; ultrastructure | English | 2021 | 2021-09 | 10.3390/ijms22189960 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Signatures of Conical Intersection Dynamics in the Time-Resolved Photoelectron Spectrum of Furan: Theoretical Modeling with an Ensemble Density Functional Theory Method | The non-adiabatic dynamics of furan excited in the pi pi* state (S-2 in the Franck-Condon geometry) was studied using non-adiabatic molecular dynamics simulations in connection with an ensemble density functional method. The time-resolved photoelectron spectra were theoretically simulated in a wide range of electron binding energies that covered the valence as well as the core electrons. The dynamics of the decay (rise) of the photoelectron signal were compared with the excited-state population dynamics. It was observed that the photoelectron signal decay parameters at certain electron binding energies displayed a good correlation with the events occurring during the excited-state dynamics. Thus, the time profile of the photoelectron intensity of the K-shell electrons of oxygen (decay constant of 34 +/- 3 fs) showed a reasonable correlation with the time of passage through conical intersections with the ground state (47 +/- 2 fs). The ground-state recovery constant of the photoelectron signal (121 +/- 30 fs) was in good agreement with the theoretically obtained excited-state lifetime (93 +/- 9 fs), as well as with the experimentally estimated recovery time constant (ca. 110 fs). Hence, it is proposed to complement the traditional TRPES observations with the trXPS (or trNEXAFS) measurements to obtain more reliable estimates of the most mechanistically important events during the excited-state dynamics. | Filatov, Michael; Lee, Seunghoon; Nakata, Hiroya; Choi, Cheol-Ho | Kyungpook Natl Univ, Dept Chem, Daegu 702701, South Korea; CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA; Kyocera, R&D Ctr Kagoshima, 1-4 Kokubu Yamashita Cho, Kirishima, Kagoshima 8994312, Japan | Choi, Cheol Ho/AAA-4705-2020; Lee, Seunghoon/AAB-4846-2021; Nakata, Hiroya/V-3205-2018 | 7005134246; 57194591254; 55305767500; 7402958948 | mike.filatov@gmail.com;slee89@caltech.edu;hiroya.nakata.gt@kyocera.jp;cheolho.choi@gmail.com; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 8 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.81 | 2025-07-30 | 11 | 11 | non-adiabatic dynamics; conical intersection; time-resolved photoelectron spectra; ionization potential; ensemble DFT | REFERENCED KOHN-SHAM; EXCITED-STATE DYNAMICS; FRACTIONALLY OCCUPIED STATES; POTENTIAL-ENERGY SURFACES; IONIZATION-POTENTIALS; ELECTRON CORRELATION; EXCITATION-ENERGIES; KOOPMANS THEOREM; HARTREE-FOCK; SPECTROSCOPY | Conical intersection; Ensemble DFT; Ionization potential; Non-adiabatic dynamics; Time-resolved photoelectron spectra | Algorithms; Density Functional Theory; Furans; Models, Molecular; Models, Theoretical; Molecular Conformation; Photoelectron Spectroscopy; furan; oxygen; furan; furan derivative; Article; density functional theory; electron; excitation; molecular dynamics; recovery time constant; simulation; theoretical model; time resolved photoelectron spectroscopy; algorithm; chemistry; conformation; density functional theory; molecular model; X ray photoemission spectroscopy | English | 2021 | 2021-04 | 10.3390/ijms22084276 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Targeting Lactate Dehydrogenase A with Catechin Resensitizes SNU620/5FU Gastric Cancer Cells to 5-Fluorouracil | Resistance to anticancer therapeutics occurs in virtually every type of cancer and becomes a major difficulty in cancer treatment. Although 5-fluorouracil (5FU) is the first-line choice of anticancer therapy for gastric cancer, its effectiveness is limited owing to drug resistance. Recently, altered cancer metabolism, including the Warburg effect, a preference for glycolysis rather than oxidative phosphorylation for energy production, has been accepted as a pivotal mechanism regulating resistance to chemotherapy. Thus, we investigated the detailed mechanism and possible usefulness of antiglycolytic agents in ameliorating 5FU resistance using established gastric cancer cell lines, SNU620 and SNU620/5FU. SNU620/5FU, a gastric cancer cell harboring resistance to 5FU, showed much higher lactate production and expression of glycolysis-related enzymes, such as lactate dehydrogenase A (LDHA), than those of the parent SNU620 cells. To limit glycolysis, we examined catechin and its derivatives, which are known anti-inflammatory and anticancer natural products because epigallocatechin gallate has been previously reported as a suppressor of LDHA expression. Catechin, the simplest compound among them, had the highest inhibitory effect on lactate production and LDHA activity. In addition, the combination of 5FU and catechin showed additional cytotoxicity and induced reactive oxygen species (ROS)-mediated apoptosis in SNU620/5FU cells. Thus, based on these results, we suggest catechin as a candidate for the development of a novel adjuvant drug that reduces chemoresistance to 5FU by restricting LDHA. | Han, Jung Ho; Kim, MinJeong; Kim, Hyeon Jin; Jang, Se Bok; Bae, Sung-Jin; Lee, In-Kyu; Ryu, Dongryeol; Ha, Ki-Tae | Pusan Natl Univ, Sch Korean Med, Dept Korean Med Sci, Yangsan 50612, South Korea; Pusan Natl Univ, Hlth Aging Korean Med Res Ctr, Yangsan 50612, South Korea; Sungkyunkwan Univ, Sch Med, Dept Mol Cell Biol, Suwon 16419, South Korea; Coll Nat Sci, Dept Mol Biol, Busan 46241, South Korea; Kyungpook Natl Univ, Dept Internal Med, Sch Med, Daegu 41566, South Korea | ; Ryu, Dongryeol/AAQ-3642-2020; Kitae, Ha/AFW-2347-2022; Bae, Sung-Jin/AAX-9144-2021; Kim, Hyeonjin/M-3761-2019; Lee, In-Kyu/AAR-6374-2021 | 57203488939; 57222729947; 57223016090; 58830948100; 57199938696; 36071537600; 57201809600; 7102262033 | hanjh1013@pusan.ac.kr;alswjd0105@skku.edu;khjkhj0903@naver.com;sbjang@pusan.ac.kr;Dr.NowOrNever@pusan.ac.kr;leei@knu.ac.kr;freefall@skku.edu;hagis@pusan.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 10 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 2.51 | 2025-07-30 | 37 | 42 | 5-fluorouracil; chemoresistant; glycolysis; lactate dehydrogenase; catechin | GREEN TEA; LDH-A; OXIDATIVE STRESS; TUMOR-GROWTH; INHIBITION; RESISTANCE; DRUG; CHEMORESISTANCE; GLYCOLYSIS; PHOSPHORYLATION | 5-fluorouracil; Catechin; Chemoresistant; Glycolysis; Lactate dehydrogenase | Apoptosis; Catechin; Cell Line, Tumor; Drug Resistance, Neoplasm; Fluorouracil; Glycolysis; Humans; Lactate Dehydrogenase 5; Reactive Oxygen Species; Stomach; Stomach Neoplasms; caspase 3; caspase 9; catechin; epigallocatechin gallate; fluorouracil; glyceraldehyde 3 phosphate dehydrogenase; lactate dehydrogenase A; lactic acid; oxamic acid; phosphoinositide dependent protein kinase 1; phosphoinositide dependent protein kinase 2; phosphoinositide dependent protein kinase 3; protein Bax; protein bcl 2; pyruvate dehydrogenase; pyruvate dehydrogenase kinase; pyruvate dehydrogenase kinase 4; reactive oxygen metabolite; unclassified drug; catechin; fluorouracil; reactive oxygen metabolite; apoptosis; Article; cancer cell; cancer chemotherapy; cancer resistance; cell proliferation; cell viability; controlled study; DNA fragmentation; enzyme kinetics; extracellular acidification rate; gene overexpression; human; human cell; isothermal titration calorimetry; LS174T cell line; MIA PaCa-2 cell line; molecular model; protein expression level; real time reverse transcription polymerase chain reaction; stomach cancer; tumor metabolism; Warburg effect; drug effect; drug resistance; glycolysis; metabolism; stomach; stomach tumor; tumor cell line | English | 2021 | 2021-05 | 10.3390/ijms22105406 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Therapeutic Effects of Inhibitor of ompA Expression against Carbapenem-Resistant Acinetobacter baumannii Strains | The widespread of carbapenem-resistant Acinetobacter baumannii (CRAB) is of great concern in clinical settings worldwide. It is urgent to develop new therapeutic agents against this pathogen. This study aimed to evaluate the therapeutic potentials of compound 62520, which has been previously identified as an inhibitor of the ompA promoter activity of A. baumannii, against CRAB isolates, both in vitro and in vivo. Compound 62520 was found to inhibit the ompA expression and biofilm formation in A. baumannii ATCC 17978 at sub-inhibitory concentrations in a dose-dependent manner. These inhibitory properties were also observed in clinical CRAB isolates belonging to sequence type (ST) 191. Additionally, compound 62520 exhibited a bacteriostatic activity against clinical clonal complex (CC) 208 CRAB isolates, including ST191, and ESKAPE pathogens. This bacteriostatic activity was not different between STs of CRAB isolates. Bacterial clearance was observed in mice infected with bioimaging A. baumannii strain 24 h after treatment with compound 62520. Compound 62520 was shown to significantly increase the survival rates of both immunocompetent and neutropenic mice infected with A. baumannii ATCC 17978. This compound also increased the survival rates of mice infected with clinical CRAB isolate. These results suggest that compound 62520 is a promising scaffold to develop a novel therapeutic agent against CRAB infections. | Na, Seok-Hyeon; Jeon, Hyejin; Oh, Man-Hwan; Kim, Yoo-Jeong; Chu, Mingi; Lee, Ill-Young; Lee, Je-Chul | Korea Dis Control & Prevent, Natl Inst Hlth, Natl Inst Infect Dis, Ctr Infect Dis Res,Div Antimicrobial Resistance R, Cheongju 28159, South Korea; Kyungpook Natl Univ, Sch Med, Dept Microbiol, Daegu 41944, South Korea; Dankook Univ, Dept Microbiol, Coll Sci & Technol, Cheonan 16890, South Korea; Korea Res Inst Chem Technol, Bio & Drug Discovery Div, Res Ctr Ecofriendly New Mat, Daejeon 34114, South Korea | 57146591600; 56822943800; 57113010400; 57145413200; 57328925300; 8843653200; 25930392000 | nash8090@korea.kr;hyejin7432@knu.ac.kr;yy1091@dankook.ac.kr;gracekim023@naver.com;mgchu@naver.com;iylee@krict.re.kr;leejc@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 22 | 22 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.81 | 2025-07-30 | 15 | 14 | Acinetobacter baumannii; ompA promoter inhibitor; compound 62520; anti-virulence; bacteriostatic agent | MEMBRANE PROTEIN-A; MULTIDRUG-RESISTANT; ANTIMICROBIAL RESISTANCE | Acinetobacter baumannii; Anti-virulence; Bacteriostatic agent; Compound 62520; OmpA promoter inhibitor | Acinetobacter baumannii; Acinetobacter Infections; Animals; Anti-Bacterial Agents; Bacterial Outer Membrane Proteins; Biofilms; Carbapenems; Drug Resistance, Multiple, Bacterial; Female; Gene Expression Regulation, Bacterial; Humans; Mice, Inbred BALB C; Microbial Sensitivity Tests; Promoter Regions, Genetic; Small Molecule Libraries; Survival Analysis; antiinfective agent; carbapenem derivative; OMPA outer membrane proteins; outer membrane protein; animal experiment; animal model; article; bacterial clearance; bacterial strain; bacteriostatic activity; bacterium isolate; biofilm; carbapenem resistant Acinetobacter baumannii; controlled study; in vitro study; in vivo study; infectious agent; inhibitory concentration; male; mouse; nonhuman; promoter region; survival rate; therapy effect; Acinetobacter baumannii; Acinetobacter infection; administration and dosage; animal; Bagg albino mouse; drug effect; female; gene expression regulation; genetics; growth, development and aging; human; metabolism; microbial sensitivity test; microbiology; molecular library; multidrug resistance; pharmacology; physiology; procedures; promoter region; survival analysis | English | 2021 | 2021-11 | 10.3390/ijms222212257 | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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