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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | ○ | Article | Phosphate level predicts mortality in acute kidney injury patients undergoing continuous kidney replacement therapy and has a U-shaped association with mortality in patients with high disease severity: a multicenter retrospective study | Background: This study investigated the association between serum phosphate level and mortality in acute kidney injury (AKI) patients undergoing continuous kidney replacement therapy (CKRT) and evaluated whether this association differed according to dis Methods: Data from eight tertiary hospitals in Korea were retrospectively analyzed. The patients were classified into four groups (low, normal, high, and very high) based on their serum phosphate level at baseline. The association between serum phosphate level and mortality was then analyzed, with further subgroup analysis being conducted according to disease severity. Results: Among the 3,290 patients identified, 166, 955, 1,307, and 862 were in the low, normal, high, and very high phosphate groups, respectively. The 90-day mortality rate was 63.9% and was highest in the very high group (76.3%). Both the high and very high groups showed a significantly higher 90-day mortality rate than did the normal phosphate group (high: hazard ratio [HR], 1.35, 95% confidence interval [CI], 1.21-1.51, p < 0.001; very high: HR, 2.01, 95% CI, 1.78-2.27, p < 0.001). The low group also exhibited a higher 90-day mortality rate than did the normal group among those with high disease severity (HR, 1.47; 95% CI, 1.09-1.99; p = 0.01) but not among those with low disease severity. Conclusion: High serum phosphate level predicted increased mortality in AKI patients undergoing CKRT, and low phosphate level was associated with increased mortality in patients with high disease severity. Therefore, serum phosphate levels should be carefully considered in critically ill patients with AKI. | Lee, Young Hwan; Lee, Soyoung; Seo, Yu Jin; Jung, Jiyun; Lee, Jangwook; Park, Jae Yoon; Ban, Tae Hyun; Park, Woo Yeong; Lee, Sung Woo; Kim, Kipyo; Kim, Kyeong Min; Kim, Hyosang; Choi, Ji-Young; Cho, Jang-Hee; Kim, Yong Chul; Lim, Jeong-Hoon | Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Div Nephrol,Dept Internal Med, 130 Dongdeok Ro, Daegu 41944, South Korea; Eulji Univ, Daejeon Eulji Med Ctr, Dept Internal Med, Div Nephrol,Sch Med, Daejeon, South Korea; Kyungpook Natl Univ, Dept Stat, Daegu, South Korea; Dongguk Univ, Data Management & Stat Inst, Ilsan Hosp, Goyang, South Korea; Dongguk Univ, Res Ctr Chron Dis & Environm Med, Coll Med, Gyeongju, South Korea; Dongguk Univ, Ilsan Hosp, Dept Internal Med, Goyang, South Korea; Dongguk Univ, Coll Med, Dept Internal Med, Gyeongju, South Korea; Catholic Univ Korea, Eunpyeong St Marys Hosp, Coll Med, Dept Internal Med, Seoul, South Korea; Keimyung Univ, Sch Med, Dept Internal Med, Dongsan Med Ctr, Daegu, South Korea; Uijeongbu Eulji Univ, Dept Internal Med, Med Ctr, Uijongbu, South Korea; Inha Univ, Dept Internal Med, Sch Med, Incheon, South Korea; Univ Ulsan, Asan Med Ctr, Dept Internal Med, Div Nephrol,Coll Med, Seoul, South Korea; Seoul Natl Univ Hosp, Dept Internal Med, 101 Daehak Ro, Seoul 03080, South Korea | Lim, Jeong-Hoon/ABE-6003-2020; Park, Woo Yeong/AGK-9140-2022; Cho, Jang-hee/ABD-3534-2020 | 59232116400; 59550701700; 59167627900; 57211813518; 57223232128; 56603383500; 56119751700; 36344980100; 56708181900; 57164118000; 57199438486; 57190118848; 7501393222; 7403536291; 57026583000; 55360244300 | imyongkim@gmail.com;jh-lim@knu.ac.kr; | KIDNEY RESEARCH AND CLINICAL PRACTICE | KIDNEY RES CLIN PRAC | 2211-9132 | 2211-9140 | 43 | 4 | SCIE | UROLOGY & NEPHROLOGY | 2024 | 3.8 | 15.4 | 1.28 | 2025-05-07 | 2 | 2 | Acute kidney injury; Continuous kidney replacement therapy; Critical illness; Mortality; Phosphates | CRITICALLY-ILL PATIENTS; HYPOPHOSPHATEMIA; FAILURE; HEMODIALYSIS; SEPSIS | Acute kidney injury; Continuous kidney replacement therapy; Critical illness; Mortality; Phosphates | creatinine; phosphate; acute kidney failure; adult; aged; Article; clinical outcome; critical illness; disease severity; disease severity assessment; estimated glomerular filtration rate; female; human; hyperkalemia; hyperphosphatemia; hypertension; major clinical study; male; mortality; mortality rate; multicenter study; phosphate blood level; receiver operating characteristic; renal replacement therapy; retrospective study; Sequential Organ Failure Assessment Score; systemic inflammation response index; urea nitrogen blood level | English | 2024 | 2024-07 | 10.23876/j.krcp.23.311 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Potentially toxic metals in seawater, sediment and seaweeds: bioaccumulation, ecological and human health risk assessment | This study assesses the bioaccumulation, ecological, and health risks associated with potentially toxic metals (PTMs), including Pb, Hg, Cd, As, and Cr in Hare Island, Thoothukudi. The results revealed that the concentration of PTMs in sediment, seawater, and S. wightii ranged from 0.095 to 2.81 mg kg-1, 0.017 to 1.515 mg L-1, and 0.076 to 5.713 mg kg-1, respectively. The highest concentrations of PTMs were found in the S. wightii compared to seawater and sediment. The high bioaccumulation of Hg and As in S. wightii suggests that it can be used as a bioindicator for these elements in this region. The ecological risk indices, which include individual, complex, biological, and ecological pollution indices, suggest that Hare Island had moderate contamination with Hg and Cd. However, there are no human health risks associated with PTMs. This study examines the current ecological and health risks associated with PTMs and emphasizes the importance of regular monitoring. | Sundhar, Shanmugam; Arisekar, Ulaganathan; Shakila, Robinson Jeya; Shalini, Rajendran; Al-Ansari, Mysoon M.; Al-Dahmash, Nora Dahmash; Mythili, R.; Kim, Woong; Sivaraman, Balasubramanian; Jenishma, J. S.; Karthy, Arjunan | Tamil Nadu Dr J Jayalalithaa Fisheries Univ TNJFU, Fisheries Coll & Res Inst, Dept Fish Qual Assurance & Management, Tuticorin 628008, Tamil Nadu, India; King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh 11451, Saudi Arabia; Saveetha Univ, Dept Pharmacol, Saveetha Inst Med & Tech Sci, Saveetha Dent Coll & Hosp, Chennai 600077, India; Kyungpook Natl Univ, Dept Environm Engn, Daegu, South Korea; Govt Tamil Nadu, Dept Fisheries & Fisherman Welf, Tuticorin, India | Shakila, Robinsondhas/AEV-6351-2022; Arisekar, Dr. Ulaganathan Arisekar/ABC-6580-2021; Al-Ansari, Mysoon/K-1915-2013; Balasubramanian, Sivaraman/GNM-5394-2022; Shanmugam, Sundhar/HOC-9720-2023 | 57210427998; 57205302340; 57212075794; 57194198054; 55266071800; 57221384176; 56765761500; 55581636400; 56898792500; 57210435784; 57443017900 | sundhar.fqm16@gmail.com;harimfsc2525@gmail.com; | ENVIRONMENTAL GEOCHEMISTRY AND HEALTH | ENVIRON GEOCHEM HLTH | 0269-4042 | 1573-2983 | 46 | 2 | SCIE | ENVIRONMENTAL SCIENCES;PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH;WATER RESOURCES;ENGINEERING, ENVIRONMENTAL | 2024 | 3.8 | 15.4 | 2.28 | 2025-05-07 | 9 | 9 | Bioaccumulation; Potentially toxic metals; Seaweed; Ecological risk; Human health risk assessment | TRACE-ELEMENT CONCENTRATION; HEAVY-METALS; SOUTHEAST COAST; SURFACE SEDIMENTS; MARINE-ENVIRONMENT; SPATIAL VARIATION; FOOD-CHAIN; POLLUTION; CONTAMINATION; GULF | Bioaccumulation; Ecological risk; Human health risk assessment; Potentially toxic metals; Seaweed | Animals; Bioaccumulation; Cadmium; Hares; Humans; Mercury; Seawater; Seaweed; India; Tamil Nadu; Tuticorin; Biochemistry; Ecology; Health risks; Mercury (metal); Risk assessment; Seawater; Seaweed; Sediments; cadmium; mercury; sea water; Ecological and human health risk assessments; Ecological pollutions; Ecological risk index; Ecological risks; Human health risk assessment; Human health risks; Pollution index; Potentially toxic metal; Thoothukudi; Toxic metals; bioaccumulation; concentration (composition); environmental risk; health risk; risk assessment; seawater; sediment analysis; toxicity; animal; bioaccumulation; hare; human; seaweed; Bioaccumulation | English | 2024 | 2024-02 | 10.1007/s10653-023-01789-0 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Removal of arsenic from jarosite waste using hydrometallurgical treatment | The jarosite waste used during this study consists of minute amount of arsenic that has a potential to be leached into environment when kept in open area. This study tried to recover arsenic from jarosite waste using hydrometallurgical treatment. The comprehensive characterization of jarosite samples was performed using various analytical techniques, including X-ray diffraction (XRD), Fourier transform Infrared (FTIR), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX), and it was characterized as natrojarosite. For optimal removal of arsenic, the response surface methodology (RSM) was applied with the key factors, including dosage (A), time (B), temperature (C), and acid concentration (D) on the recovery of arsenic. The results indicated that the dosage (A) and acid concentration (D) demonstrated significant positive effects on arsenic recovery. As expected, the higher dosage and acid concentration was associated with increased recovery percentages for the arsenic from jarosite. Whereas time (B) and temperature (C) did not exhibit statistically significant recovery of arsenic within the specified experimental range. The contour plots showed the optimal operating conditions for the highest recovery percentage was approximately 52.61% when 2.5 g of jarosite was treated with 10 mol/L acid for 150 min at operating temperature of 80 degrees. Although our study showed very moderate recovery of arsenic, it is first report where arsenic has been removed from jarosite waste. Readjustment of range of operating parameters would provide more insight into the further optimization of the yield. | Singh, Vishal Kumar; Kumar, Mukul; Manna, Suvendu; Bobde, Prakash; Govarthanan, Muthusamy | Univ Petr & Energy Studies, Sch Adv Engn, Sustainabil Cluster, Dehra Dun 248007, Uttarakhand, India; Univ Petr & Energy Studies, Sch Hlth Sci & Technol, Dept Microbiol, Dehra Dun 248007, Uttarakhand, India; Univ Petr & Energy Studies, Res & Dev Sect, Dehra Dun 248007, Uttarakhand, India; Kyungpook Natl Univ, 80 Daehak Ro, Daegu 41566, South Korea; Saveetha Univ, Dept Biomat, Saveetha Inst Med & Tech Sci, Saveetha Dent Coll & Hosp, Chennai 600077, Tamil Nadu, India | ; Kumar, Mukul/JRY-1192-2023; Bobde, Prakash/AAW-8073-2020; Singh, Vishal/GZN-1006-2022; Muthusamy, Govarthanan/C-1491-2014 | 57213256284; 58881619400; 35885832600; 57221316092; 54881927600 | smanna@ddn.upes.ac.in;suva84@gmail.com; | ENVIRONMENTAL GEOCHEMISTRY AND HEALTH | ENVIRON GEOCHEM HLTH | 0269-4042 | 1573-2983 | 46 | 2 | SCIE | ENVIRONMENTAL SCIENCES;PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH;WATER RESOURCES;ENGINEERING, ENVIRONMENTAL | 2024 | 3.8 | 15.4 | 0.38 | 2025-05-07 | 1 | 1 | Arsenic; Acid treatment; Response surface methodology; Central composite design | VANADIUM EXTRACTION; ACID; OPTIMIZATION; DISSOLUTION; RECOVERY; DISPOSAL; CEMENT | Acid treatment; Arsenic; Central composite design; Response surface methodology | Energy dispersive spectroscopy; Fourier transform infrared spectroscopy; Hydrometallurgy; Recovery; Scanning electron microscopy; Surface properties; Waste treatment; Acid concentrations; Acid treatments; Central composite designs; Fourier transform infrared; Hydrometallurgical treatments; Jarosites; Recovery percentages; Removal of arsenics; Response-surface methodology; X- ray diffractions; arsenic; jarosite; pollutant removal; response surface methodology; waste; Arsenic | English | 2024 | 2024-02 | 10.1007/s10653-024-01868-w | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Structural Effect of Cyclic Olefin Cross-Linkers on Long-Wave Infrared-Transmitting Sulfur Polymers | The structural characteristics of the organic cross-linker greatly affect the potential infrared (IR) optics applications of sulfur polymers synthesized through the inverse vulcanization reaction. Unlike elemental sulfur, organic cross-linkers induce various absorptions in the IR region and leave organic moieties that affect the IR transmittance, even after inverse vulcanization. Most of the cyclic olefin cross-linkers [e.g., dicyclopentadiene (DCPD)] investigated so far are expected to produce side reactions and form byproducts during inverse vulcanization. The side reactions caused by differences in reactivity between the reaction sites can result in the deterioration of the optical properties of sulfur polymers. In this study, thiol groups were introduced as a cross-linker to effectively control the side reactions and byproducts that may occur in inverse vulcanization. The sulfur and thiol cross-linkers reacted rapidly and uniformly at all reaction sites to form sulfur polymers without unwanted side reactions. Optical windows prepared with the sulfur polymer exhibited enhanced IR transparency and achieved thermal imaging of the human body in the long-wave IR (LWIR) region. By design of a cross-linker for LWIR transparent sulfur polymers, these results provide a useful solution for IR optics applications. | Lee, Miyeon; Jang, Se Gyu; Yeo, Hyeonuk; Park, Jong-Jin; Moon, Bongjin; You, Nam-Ho | Korea Inst Sci & Technol KIST, Carbon Composite Mat Res Ctr, Wonju 55324, South Korea; Korea Inst Sci & Technol KIST, Funct Composite Mat Res Ctr, Wonju 55324, South Korea; Kyungpook Natl Univ, Dept Chem Educ, Dept Nanosci & Nanotechnol, Dept Pharm, Daegu 41566, South Korea; Chonnam Natl Univ, Dept Polymer Engn, Gwangju 61186, South Korea; Sogang Univ, Dept Chem, Seoul 04107, South Korea | ; Yeo, Hyeonuk/AHE-0397-2022; yeo, hyeonuk/G-7890-2017 | 57214328880; 7402219010; 55324816500; 59871668100; 7101878648; 55204428300 | polymer@kist.re.kr; | MACROMOLECULES | MACROMOLECULES | 0024-9297 | 1520-5835 | 57 | 6 | SCIE | POLYMER SCIENCE | 2024 | 5.2 | 15.4 | 1.58 | 2025-05-07 | 5 | 4 | ELEMENTAL SULFUR; INVERSE VULCANIZATION; CATHODE MATERIALS; POLYMERIZATIONS; POLYSULFIDE | Crosslinking; Deterioration; Infrared devices; Infrared imaging; Infrared radiation; Olefins; Optical properties; Rubber; Vulcanization; Crosslinker; Cyclic olefins; Infrared optics; Longwave infrared; Optics application; Organics; Reaction sites; Side reactions; Structural effect; Vulcanisation; Sulfur | English | 2024 | 2024-03-11 | 10.1021/acs.macromol.4c00018 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | The impact of severe depression on the survival of older patients with end-stage kidney disease | Background: Incidence of depression increases in patients with end-stage kidney disease (ESKD). We evaluated the association between depression and mortality among older patients with ESKD, which has not been studied previously. Methods: This nationwide prospective cohort study included 487 patients with ESKD aged >65 years, who were categorized into minimal, mild-to-moderate, and severe depression groups based on their Beck Depression Inventory-II (BDI-II) scores. Predisposing factors for high BDI-II scores and the association between the scores and survival were analyzed. Results: The severe depression group showed a higher modified Charlson comorbidity index value and lower serum albumin, phosphate, and uric acid levels than the other depression groups. The Kaplan-Meier curve revealed a significantly lower survival in the severe depression group than in the minimal and mild-to-moderate depression groups (p = 0.011). Multivariate Cox regression analysis confirmed that severe depression was an independent risk factor for mortality in the study cohort (hazard ratio, 1.39; 95% confidence interval, 1.01-1.91; p = 0.041). Additionally, BDI-II scores were associated with modified Charlson comorbidity index (p = 0.009) and serum albumin level (p = 0.004) in multivariate linear regression. Among the three depressive symptoms, higher somatic symptom scores were associated with increased mortality. Conclusion: Severe depression among older patients with ESKD increases mortality compared with minimal or mild-to-moderate depression, and patients with concomitant somatic symptoms require careful management of their comorbidities and nutritional status. | Jeon, You Hyun; Lim, Jeong-Hoon; Jeon, Yena; Chung, Yu-Kyung; Kim, Yon Su; Kang, Shin-Wook; Yang, Chul Woo; Kim, Nam-Ho; Jung, Hee-Yeon; Choi, Ji-Young; Park, Sun-Hee; Kim, Chan-Duck; Kim, Yong-Lim; Cho, Jang-Hee | Kyungpook Natl Univ, Sch Med, Dept Internal Med, Div Nephrol,Kyungpook Natl Univ Hosp, Daegu, South Korea; Clin Res Ctr End Stage Renal Dis, Taegu, South Korea; Kyungpook Natl Univ, Coll Nat Sci, Dept Stat, Daegu, South Korea; Seoul Natl Univ, Coll Med, Dept Internal Med, Seoul, South Korea; Yonsei Univ, Coll Med, Dept Internal Med, Seoul, South Korea; Catholic Univ Korea, Coll Med, Dept Internal Med, Seoul, South Korea; Chonnam Natl Univ, Med Sch, Dept Internal Med, Gwangju, South Korea | Cho, Jang-hee/ABD-3534-2020; Kim, Yong-Lim/AGK-3172-2022; Lim, Jeong-Hoon/ABE-6003-2020; Park, Sun-Hee/LMN-0033-2024 | 57820096000; 55360244300; 57209909350; 57289628500; 56066702900; 57211696347; 58304773000; 56605215700; 57196396467; 7501393222; 7501831741; 59216189400; 55633533600; 7403536291 | ylkim@knu.ac.kr;jh-cho@knu.ac.kr; | KIDNEY RESEARCH AND CLINICAL PRACTICE | KIDNEY RES CLIN PRAC | 2211-9132 | 2211-9140 | 43 | 6 | SCIE | UROLOGY & NEPHROLOGY | 2024 | 3.8 | 15.4 | 0.64 | 2025-05-07 | 1 | 2 | Comorbidity; Depression; Dialysis; Aged; Survival | QUALITY-OF-LIFE; RENAL REPLACEMENT THERAPY; HEMODIALYSIS-PATIENTS; ALL-CAUSE; MORTALITY; SYMPTOMS; HOSPITALIZATION; DIAGNOSIS; DIALYSIS; ILLNESS | Aged; Comorbidity; Depression; Dialysis; Survival | albumin; C reactive protein; creatinine; ferritin; hemoglobin; phosphate; uric acid; aged; all cause mortality; anxiety; Article; Beck Depression Inventory; body mass; body weight loss; cerebrovascular disease; Charlson Comorbidity Index; chronic lung disease; cohort analysis; comorbidity; depression; end stage renal disease; fatigue; female; hemodialysis; human; major clinical study; male; mortality; prospective study; risk factor; sleep disorder; survival; uric acid blood level | English | 2024 | 2024-11 | 10.23876/j.krcp.22.268 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Waveguide-Thru Closed-Form Characterization of Anisotropic Polymer Network Liquid-Crystal for mmWave Reconfigurable RF Devices | In this article, closed-form characterization of anisotropic polymer network liquid-crystal (PNLC) for reconfigurable RF devices at the millimeter-wave (mmWave) band is achieved. For the first time, the complex permittivity of the PNLC has been extracted for various doping conditions of reactive mesogen used in the polymer network at the mmWave band. Anisotropic constitutive parameters at the two extreme states of the PNLC can be obtained through a waveguide (WG). A 1 x 2 reflectarray simple cell in the WG enables the establishment of an equivalent circuit (EQ) for an infinite cell array, providing exact modeling for de-embedding. The PNLC has an attractive property compared with pristine nematic liquid crystals (NLCs) owing to its significantly enhanced dynamic switching characteristics. In the mmWave band, the physical cell gap of the liquid crystal (LC) inevitably increases to achieve RF performance, resulting in slow dynamic speed. However, studies on the characterization of PNLCs at the mmWave band are limited, and their evaluation methodologies are inaccurate. The fabricated cell requires no biasing circuit to achieve two extreme phase states, owing to its prealigned layer. With the proposed novel method, constitutive parameters of other LC types, modified by doping approaches for RF performance can be precisely extracted and predicted. | Kim, Hogyeom; Lee, Jae-Won; Wang, Junkai; Kim, Min-Seok; Kim, Hak-Rin; Oh, Jungsuek | Seoul Natl Univ, Inst New Media & Commun INMC, Seoul 08826, South Korea; Seoul Natl Univ, Dept Elect & Comp Engn, Seoul 08826, South Korea; Kyungpook Natl Univ, Sch Elect & Elect Engn, Daegu 41566, South Korea; Kyungpook Natl Univ, Sch Elect Engn, Daegu 41566, South Korea | Wang, Junkai/KIK-4047-2024; Kim, Hak-Rin/T-1897-2019 | 57210172464; 58377059800; 57957421500; 59073001500; 7410124944; 35194285700 | rineey@knu.ac.kr;jungsuek@snu.ac.kr; | IEEE TRANSACTIONS ON ANTENNAS AND PROPAGATION | IEEE T ANTENN PROPAG | 0018-926X | 1558-2221 | 72 | 7 | SCIE | ENGINEERING, ELECTRICAL & ELECTRONIC;TELECOMMUNICATIONS | 2024 | 5.8 | 15.4 | 0 | 2025-04-16 | 0 | 3 | Dynamic switching time; liquid crystal (LC); polymer network liquid crystal (PNLC); reactive mesogen; Dynamic switching time; liquid crystal (LC); polymer network liquid crystal (PNLC); reactive mesogen | MILLIMETER-WAVE; ANTENNA; MODEL; FREQUENCY; CELLS | Dynamic switching time; liquid crystal (LC); polymer network liquid crystal (PNLC); reactive mesogen | Anisotropy; Antenna arrays; Cells; Equivalent circuits; Millimeter waves; Nematic liquid crystals; Optical communication; Permittivity; Waveguides; Dynamic switching; Dynamic switching time; Liquid-crystals; Millimeterwave communications; Polymer network liquid crystal; Polymer networks; Radiofrequencies; Reactive mesogens; Switching time; Cytology | English | 2024 | 2024-07 | 10.1109/tap.2024.3403939 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Activation of neurotoxic A1-reactive astrocytes by SFTS virus infection accelerates fatal brain damage in IFNAR1⁻/⁻ mice | Severe fever with thrombocytopenia syndrome (SFTS) has a high mortality rate compared to other infectious diseases. SFTS is particularly associated with a high risk of mortality in immunocompromised individuals, while most patients who die of SFTS exhibit symptoms of severe encephalitis before death. However, the region of brain damage and mechanisms by which the SFTS virus (SFTSV) causes encephalitis remains unknown. Here, we revealed that SFTSV infects the brainstem and spinal cord, which are regions of the brain associated with respiratory function, and motor nerves in IFNAR1(-/-) mice. Further, we show that A1-reactive astrocytes are activated, causing nerve cell death, in infected mice. Primary astrocytes of SFTSV-infected IFNAR1(-/-) mice also induced neuronal cell death through the activation of A1-reactive astrocytes. Herein, we showed that SFTSV induces fatal neuroinflammation in the brain regions important for respiratory function and motor nerve, which may underlie mortality in SFTS patients. This study provides new insights for the treatment of SFTS, for which there is currently no therapeutic approach. | Kim, Seon-Hee; Choi, Ha Nyeoung; Jo, Min Gi; Lee, Bina; Kim, Young Jin; Seong, Hyemin; Song, Chieun; Yoo, Han Sol; Lee, Jeong Hyun; Seong, Daseul; Park, Hyun-Jin; Roh, In-Soon; Yang, Jinsung; Lee, Min Young; Kim, Hye Jung; Park, Sang Won; Kim, Mingyo; Kim, Seong Jae; Kim, Minkyeong; Kim, Hyun-Jeong; Hong, Kyung-Wook; Yun, Seung Pil | Gyeongsang Natl Univ, Coll Med, Inst Med Sci, Dept Pharmacol, Jinju 52727, South Korea; Gyeongsang Natl Univ, Coll Med, Dept Convergence Med Sci, Jinju, South Korea; Kyung Hee Univ, Coll Med, Dept Pathol, Seoul, South Korea; Gyeongsang Natl Univ, Inst Med Sci, Coll Med, Dept Ophthalmol, Jinju, South Korea; Anim & Plant Quarantine Agcy, Div Foreign Anim Dis, Gimcheon 39660, South Korea; Gyeongsang Natl Univ, Inst Med Sci, Coll Med, Dept Biochem, Jinju, South Korea; Kyungpook Natl Univ, Coll Pharm, Daegu, South Korea; Gyeongsang Natl Univ Hosp, Dept Rheumatol Internal Med, Jinju, South Korea; Gyeongsang Natl Univ Hosp, Dept Neurol, Jinju, South Korea; Gyeongsang Natl Univ, Lab Anim Res Ctr, Cent Sci Instrumentat Facil, Jinju, South Korea; Gyeongsang Natl Univ, Gyeongsang Natl Univ Hosp, Dept Internal Med, Div Infect Dis,Coll Med, Jinju 52727, South Korea | Park, Hyun-Jin/HPE-8152-2023; Lee, JH/JMB-8516-2023; Hong, Kyung-Wook/T-3067-2018; Kim, Young-jin/GSD-3168-2022 | 59104096700; 59131798800; 57204517713; 55839741900; 59132011800; 56585536200; 58488441900; 58932472400; 58932698400; 58843402900; 59257659600; 22935810200; 57211162448; 59058829100; 55793607500; 56588162900; 56029429600; 55578336500; 57203524825; 59867675500; 38461203300; 25951635400 | hjkim1945@gnu.ac.kr;resina78@naver.com;spyun@gnu.ac.kr; | JOURNAL OF MEDICAL VIROLOGY | J MED VIROL | 0146-6615 | 1096-9071 | 96 | 8 | SCIE | VIROLOGY | 2024 | 4.6 | 15.5 | 0.66 | 2025-05-07 | 1 | 1 | astrocytes; CNS symptoms; neuroinflammation; severe fever with thrombocytopenia syndrome (SFTS); viral infection | THROMBOCYTOPENIA SYNDROME; SEVERE FEVER; PATHOGENESIS; INTERFERON; BUNYAVIRUS | astrocytes; CNS symptoms; neuroinflammation; severe fever with thrombocytopenia syndrome (SFTS); viral infection | Animals; Astrocytes; Brain; Brain Stem; Bunyaviridae Infections; Cell Death; Disease Models, Animal; Mice; Mice, Inbred C57BL; Mice, Knockout; Neurons; Phlebovirus; Receptor, Interferon alpha-beta; Spinal Cord; interleukin 1alpha; interleukin 1beta; interleukin 6; nucleocapsid protein; tumor necrosis factor; alpha beta interferon receptor; Ifnar1 protein, mouse; animal cell; animal experiment; animal model; animal tissue; Article; astrocyte; brain damage; brain stem; cell activation; cell death; cell viability; controlled study; encephalitis; genotype; glia cell; immunofluorescence; immunohistochemistry; microglia; motor nerve; mouse; nerve cell; nerve cell necrosis; nervous system inflammation; neurotoxic A1 reactive astrocyte; nonhuman; olfactory bulb; real time reverse transcription polymerase chain reaction; severe fever with thrombocytopenia syndrome; Severe fever with thrombocytopenia syndrome virus; spinal cord; TUNEL assay; virus infection; animal; brain; bunyavirus infection; C57BL mouse; disease model; genetics; immunology; knockout mouse; pathogenicity; pathology; Phlebovirus; physiology; virology | English | 2024 | 2024-08 | 10.1002/jmv.29854 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Functional modulation of lysophosphatidic acid type 2 G-protein coupled receptor facilitates alveolar bone formation | Lipid biosynthesis is recently studied its functions in a range of cellular physiology including differentiation and regeneration. However, it still remains to be elucidated in its precise function. To reveal this, we evaluated the roles of lysophosphatidic acid (LPA) signaling in alveolar bone formation using the LPA type 2 receptor (LPAR2) antagonist AMG-35 (Amgen Compound 35) using tooth loss without periodontal disease model which would be caused by trauma and usually requires a dental implant to restore masticatory function. In this study, in vitro cell culture experiments in osteoblasts and periodontal ligament fibroblasts revealed cell type-specific responses, with AMG-35 modulating osteogenic differentiation in osteoblasts in vitro. To confirm the in vivo results, we employed a mouse model of tooth loss without periodontal disease. Five to 10 days after tooth extraction, AMG-35 facilitated bone formation in the tooth root socket as measured by immunohistochemistry for differentiation markers KI67, Osteocalcin, Periostin, RUNX2, transforming growth factor beta 1 (TGF-beta 1) and SMAD2/3. The increased expression and the localization of these proteins suggest that AMG-35 elicits osteoblast differentiation through TGF-beta 1 and SMAD2/3 signaling. These results indicate that LPAR2/TGF-beta 1/SMAD2/3 represents a new signaling pathway in alveolar bone formation and that local application of AMG-35 in traumatic tooth loss can be used to facilitate bone regeneration and healing for further clinical treatment. | Kim, Tae-Young; Kim, Anna; Aryal, Yam Prasad; Sung, Shijin; Pokharel, Elina; Neupane, Sanjiv; Choi, So-Young; Ha, Jung-Hong; Jung, Jae-Kwang; Yamamoto, Hitoshi; An, Chang-Hyeon; Suh, Jo-Young; Sohn, Wern-Joo; Lee, Youngkyun; Jang, Il-Ho; Norman, Derek D.; Tigyi, Gabor J.; An, Seo-Young; Kim, Jae-Young | Kyungpook Natl Univ, Sch Dent, IHBR, Dept Biochem, Daegu, South Korea; SUNY Stony Brook, Dept Biochem & Cell Biol, Stony Brook, NY USA; Kyungpook Natl Univ, Sch Dent, IHBR, Dept Oral & Maxillofacial Surg, Daegu, South Korea; Kyungpook Natl Univ, Sch Dent, IHBR, Dept Conservat Dent, Daegu, South Korea; Kyungpook Natl Univ, Sch Dent, IHBR, Dept Oral Med, Daegu, South Korea; Tokyo Dent Coll, Dept Histol & Dev Biol, Tokyo, Japan; Kyungpook Natl Univ, Sch Dent, IHBR, Dept Oral & Maxillofacial Radiol, Daegu, South Korea; Kyungpook Natl Univ, Sch Dent, IHBR, Dept Periodontol, Daegu, South Korea; Daegu Hanny Univ, Coll Cosmet & Pharmaceut, Dept K Beauty Business, Gyongsan, South Korea; Pusan Natl Univ, Sch Dent, Inst Translat Dent Sci, Dept Oral Biochem & Mol Biol, Yangsan, South Korea; Univ Tennessee, Hlth Sci Ctr, Dept Physiol, Memphis, TN USA; Kyungpook Natl Univ, Sch Dent, IHBR, Dept Biochem, 2177,Dalgubeol Daero, Daegu 41940, South Korea; Kyungpook Natl Univ, Sch Dent, IHBR, Dept Oral & Maxillofacial Radiol, 2177,Dalgubeol Daero, Daegu 41940, South Korea | Kim, Ji-Youn/A-5779-2017; Kim, AJ/LIG-4661-2024; Neupane, Sanjiv/N-4460-2019; Tigyi, Gabor/LZF-2742-2025 | 57208461628; 58112989700; 57202611163; 55787126100; 57220028220; 56183800400; 57202918688; 55549831900; 55970994400; 55725330600; 17134437600; 7201514992; 44161404800; 36062942200; 35083663000; 37461777300; 7005779169; 55258203200; 56812734700 | syan@knu.ac.kr;jykim91@knu.ac.kr; | JOURNAL OF CELLULAR PHYSIOLOGY | J CELL PHYSIOL | 0021-9541 | 1097-4652 | 239 | 1 | SCIE | CELL BIOLOGY;PHYSIOLOGY | 2024 | 4 | 15.5 | 1.22 | 2025-04-16 | 2 | 2 | G-protein-coupled receptors; lysophosphatidic acid receptors; osteoblasts; SMAD2/3 proteins; transforming growth factor beta1 | DIFFERENTIATION; TGF-BETA-1; PROMOTE; CELLS | G-protein-coupled receptors; lysophosphatidic acid receptors; osteoblasts; SMAD2/3 proteins; transforming growth factor beta1 | Animals; Cell Differentiation; Lysophospholipids; Mice; Osteoblasts; Osteogenesis; Periodontal Ligament; Tooth Loss; Transforming Growth Factor beta1; alkaline phosphatase; dimethyl sulfoxide; eosin; G protein coupled receptor; hematoxylin; Ki 67 antigen; lysophosphatidic acid receptor; osteocalcin; Smad2 protein; Smad3 protein; transcription factor RUNX2; transforming growth factor beta1; lysophosphatidic acid; lysophospholipid; transforming growth factor beta1; alveolar bone; animal cell; animal experiment; animal model; Article; bone regeneration; bone remodeling; bone tissue; cell culture; cell differentiation; cell proliferation; cell viability; cells; controlled study; fibroblast; immunohistochemistry; immunoreactivity; in vitro study; in vivo study; MC3T3 cell line; morphogenesis; MTS assay; nonhuman; ossification; osteoblast; osteoclast; periodontal disease; periodontal ligament; protein expression; signal transduction; tooth extraction; tooth root; tooth socket; animal; bone development; metabolism; mouse; periodontal disease; physiology | English | 2024 | 2024-01 | 10.1002/jcp.31148 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | GULP1 deficiency reduces adipogenesis and glucose uptake via downregulation of PPAR signaling and disturbing of insulin/ERK signaling in 3T3-L1 cells | Obesity and metabolic disorders caused by alterations in lipid metabolism are major health issues in developed, affluent societies. Adipose tissue is the only organ that stores lipids and prevents lipotoxicity in other organs. Mature adipocytes can affect themselves and distant metabolism-related tissues by producing various adipokines, including adiponectin and leptin. The engulfment adaptor phosphotyrosine-binding domain-containing 1 (GULP1) regulates intracellular trafficking of glycosphingolipids and cholesterol, suggesting its close association with lipid metabolism. However, the role of GULP1 in adipocytes remains unknown. Therefore, this study aimed to investigate the function of GULP1 in adipogenesis, glucose uptake, and the insulin signaling pathway in adipocytes. A 3T3-L1 cell line with Gulp1 knockdown (shGulp1) and a 3T3-L1 control group (U6) were established. Changes in shGulp1 cells due to GULP1 deficiency were examined and compared to those in U6 cells using microarray analysis. Glucose uptake was monitored via insulin stimulation in shGulp1 and U6 cells using a 2-NBDG glucose uptake assay, and the insulin signaling pathway was investigated by western blot analysis. Adipogenesis was significantly delayed, lipid metabolism was altered, and several adipogenesis-related genes were downregulated in shGulp1 cells compared to those in U6 cells. Microarray analysis revealed significant inhibition of peroxisome proliferator-activated receptor signaling in shGulp1 cells compared with U6 cells. The production and secretion of adiponectin as well as the expression of adiponectin receptor were decreased in shGulp1 cells. In particular, compared with U6 cells, glucose uptake via insulin stimulation was significantly decreased in shGulp1 cells through the disturbance of ERK1/2 phosphorylation. This is the first study to identify the role of GULP1 in adipogenesis and insulin-stimulated glucose uptake by adipocytes, thereby providing new insights into the differentiation and functions of adipocytes and the metabolism of lipids and glucose, which can help better understand metabolic diseases. | Kim, Soon-Young; Park, Seung-Yoon; Kim, Jung-Eun | Kyungpook Natl Univ, Cell & Matrix Res Inst, Sch Med, Dept Mol Med, 680 Gukchaebosang Ro, Daegu 41944, South Korea; Dongguk Univ, Sch Med, Dept Biochem, Gyeongju, South Korea; Kyungpook Natl Univ, Dept Biomed Sci, BK21 Four KNU Convergence Educ Program Biomed Sci, Daegu, South Korea | Kim, Sang/HSD-0402-2023 | 57204021560; 8627776300; 57209054588 | kjeun@knu.ac.kr; | JOURNAL OF CELLULAR PHYSIOLOGY | J CELL PHYSIOL | 0021-9541 | 1097-4652 | 239 | 2 | SCIE | CELL BIOLOGY;PHYSIOLOGY | 2024 | 4 | 15.5 | 0 | 2025-05-07 | 2 | 1 | adipogenesis; adiponectin; glucose uptake; GULP1; insulin signaling; PPAR signaling | CASSETTE TRANSPORTER A1; PROTEIN GULP; ALPHA; ENGULFMENT; EXPRESSION; TISSUE | adipogenesis; adiponectin; glucose uptake; GULP1; insulin signaling; PPAR signaling | 3T3-L1 Cells; Adipogenesis; Adiponectin; Animals; Cell Differentiation; Down-Regulation; Glucose; Insulin; Lipids; Mice; Peroxisome Proliferator-Activated Receptors; PPAR gamma; Signal Transduction; adaptor protein; adipocytokine; adiponectin; adiponectin receptor; cell protein; engulfment adaptor phosphotyrosine binding domain containing 1 protein; fatty acid binding protein 4; insulin; lipoprotein lipase; mitogen activated protein kinase 1; mitogen activated protein kinase 3; unclassified drug; adiponectin; glucose; insulin; lipid; peroxisome proliferator activated receptor; peroxisome proliferator activated receptor gamma; 2 NBDG glucose uptake assay; 3T3-L1 cell line; adipo q gene; adipocyte; adipogenesis; adipose tissue; animal tissue; Article; assay; cell function; cell stimulation; controlled study; down regulation; enzyme inhibition; enzyme phosphorylation; gene; gene knockdown; glucose transport; insulin signaling; knockout mouse; lipid metabolism; male; metabolic disorder; microarray analysis; mouse; nonhuman; PPAR signaling; protein deficiency; protein expression; protein function; protein secretion; signal transduction; U6 cell line; Western blotting; wild type mouse; animal; cell differentiation; genetics; metabolism | English | 2024 | 2024-02 | 10.1002/jcp.31173 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | RFX4 is an intrinsic factor for neuronal differentiation through induction of proneural genes POU3F2 and NEUROD1 | Proneural genes play a crucial role in neuronal differentiation. However, our understanding of the regulatory mechanisms governing proneural genes during neuronal differentiation remains limited. RFX4, identified as a candidate regulator of proneural genes, has been reported to be associated with the development of neuropsychiatric disorders. To uncover the regulatory relationship, we utilized a combination of multi-omics data, including ATAC-seq, ChIP-seq, Hi-C, and RNA-seq, to identify RFX4 as an upstream regulator of proneural genes. We further validated the role of RFX4 using an in vitro model of neuronal differentiation with RFX4 knock-in and a CRISPR-Cas9 knock-out system. As a result, we found that RFX4 directly interacts with the promoters of POU3F2 and NEUROD1. Transcriptomic analysis revealed a set of genes associated with neuronal development, which are highly implicated in the development of neuropsychiatric disorders, including schizophrenia. Notably, ectopic expression of RFX4 can drive human embryonic stem cells toward a neuronal fate. Our results strongly indicate that RFX4 serves as a direct upstream regulator of proneural genes, a role that is essential for normal neuronal development. Impairments in RFX4 function could potentially be related to the development of various neuropsychiatric disorders. However, understanding the precise mechanisms by which the RFX4 gene influences the onset of neuropsychiatric disorders requires further investigation through human genetic studies. | Choi, Wonyoung; Choe, Mu Seog; Kim, Su Min; Kim, So Jin; Lee, Jiyeon; Lee, Yeongun; Lee, Sun-Min; Dho, So Hee; Lee, Min-Young; Kim, Lark Kyun | Yonsei Univ, Grad Sch, Interdisciplinary Program Integrated OM Biomed Sci, Seoul, South Korea; Kyungpook Natl Univ, Res Inst Pharmaceut Sci, Coll Pharm, Daegu, South Korea; Yonsei Univ, Gangnam Severance Hosp, Grad Sch Med Sci, Dept Biomed Sci,Coll Med, Seoul 06230, South Korea; Konkuk Univ, Dept Phys, Seoul, South Korea | ; Kim, Byung/L-6884-2019; Choi, Wonyoung/LMP-4209-2024; Kim, Sumin/IZE-4757-2023 | 56147288900; 57202926165; 57219516843; 57224776382; 57211588445; 57455107100; 57207065244; 58352388800; 15119890400; 55747965100 | vetmedic@knu.ac.kr;LKKIM@yuhs.ac; | CELLULAR AND MOLECULAR LIFE SCIENCES | CELL MOL LIFE SCI | 1420-682X | 1420-9071 | 81 | 1 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CELL BIOLOGY | 2024 | 6.2 | 15.5 | 0.46 | 2025-05-07 | 4 | 3 | RFX; Proneural factor; Epigenome; Neuropsychiatric disorders; Neuronal differentiation; Stem cell | TRANSCRIPTION FACTORS; CHROMATIN ACCESSIBILITY; CELLS; FIBROBLASTS; BINDING; MOUSE; ACTIVATORS; CONVERSION; FAMILY | Epigenome; Neuronal differentiation; Neuropsychiatric disorders; Proneural factor; RFX; Stem cell | Basic Helix-Loop-Helix Transcription Factors; Gene Expression Profiling; Humans; Intrinsic Factor; Promoter Regions, Genetic; RNA-Seq; neurogenic differentiation factor; protein POU3F2; regulatory factor X transcription factor; regulatory factor X4; transcription factor; unclassified drug; Article; assay for transposase accessible chromatin sequencing; cell fate; chromatin immunoprecipitation sequencing; chromosome analysis; controlled study; CRISPR-CAS9 system; ectopic expression; embryo; gene induction; gene knock-in; gene knockout; high throughput chromosome conformation capture; human; human cell; human embryonic stem cell; human genetics; mental disease; molecular pathology; multiomics; nerve cell differentiation; nervous system development; NEUROD1 gene; POU3F2 gene; promoter region; protein analysis; protein DNA interaction; protein function; RNA sequencing; schizophrenia; sequence analysis; transcriptomics; article; clustered regularly interspaced short palindromic repeat; epigenome; etiology; fetus; gene; in vitro study; male; schizophrenia; stem cell | English | 2024 | 2024-12 | 10.1007/s00018-024-05129-y | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Development of wide-field high-resolution dual optical imaging platform for vasculature and morphological assessment of chronic kidney disease: A feasibility study | Chronic kidney disease (CKD) affects the morphological structure and causes significant degradation in kidney function, leading to renal replacement treatment in affected individuals. Vascular rarefaction is thought to be an important factor in accelerating kidney damage in CKD patients, therefore, the assessment of renal morphology and vasculature is crucial in nephrology. The objective of this study was to evaluate the morphological and vascular changes caused by CKD in mice kidneys. In this study, dual photoacoustic microscopy (PAM) and optical coherence microscopy (OCM) oriented wide-field high-resolution imaging modalities were employed for diseased renal imaging. The unilateral ureteral obstruction (UUO) model was used to prepare renal samples with CKD, and the developed wide-field dual imaging system was used to image both control and CKD-affected kidneys for assessing vascular and morphological changes during CKD progression. The obtained results reveal a gradual alteration in vascular intensity and pelvis space with the progress of UUO disease. Furthermore, a quantitative micro-vessel analysis was performed based on the node, junction, and mesh of the vessel, which provides details on the increasing microvascular-related characteristics in the peripheral area as the disease progresses. Thus, by concurrently employing the advantages of each optical imaging technique, the proposed method of assessing the OCM-based morphological and PAM-based vascular properties of the renal sample using a wide-field multimodal imaging system can be an efficient technique for whole volume analysis without any exogenous contrast agents in kidney histopathology. | Abu Saleah, Sm; Lee, Jaeyul; Seong, Daewoon; Han, Sangyeob; Park, Kibeom; Hong, Juyeon; Park, Sooah; Kwon, Yoon-Hee; Jung, Woonggyu; Jeon, Mansik; Kim, Jeehyun | Kyungpook Natl Univ, Coll IT Engn, Sch Elect & Elect Engn, 80,Daehak ro, Daegu 41566, South Korea; Massachusetts Gen Hosp, Div Pulm & Crit Care Med, Boston, MA 02114 USA; Harvard Med Sch, Boston, MA 02114 USA; Washington Univ St Louis, McKelvey Sch Engn, Dept Biomed Engn, St. Louis, MO 63130 USA; Ulsan Natl Inst Sci & Technol UNIST, Vivo Res Ctr, UNIST Cent Res Facil, Ulsan 44919, South Korea; Ulsan Natl Inst Sci & Technol, Dept Biomed Engn, 50 UNIST gil, Ulsan 44919, South Korea | Lee, Jung Bok/HHZ-3200-2022 | 59154305300; 57188689420; 57212512353; 57193695305; 56017776400; 58157239900; 57208579586; 58210060700; 58291338100; 24171094000; 59510666600 | msjeon@knu.ac.kr; | BIOCYBERNETICS AND BIOMEDICAL ENGINEERING | BIOCYBERN BIOMED ENG | 0208-5216 | 44 | 3 | SCIE | ENGINEERING, BIOMEDICAL | 2024 | 6.6 | 15.7 | 0 | 2025-05-07 | 0 | 0 | Chronic kidney disease; Optical coherence microscopy; Photoacoustic microscopy; Unilateral ureteral obstruction; Disease model | MICRO-COMPUTED TOMOGRAPHY; COHERENCE TOMOGRAPHY; INDUCED NEPHROPATHY; RENAL DYSFUNCTION; ASCORBIC-ACID; ULTRASOUND; FIBROSIS; INJURY; PATHOPHYSIOLOGY; QUANTIFICATION | Chronic kidney disease; Disease model; Optical coherence microscopy; Photoacoustic microscopy; Unilateral ureteral obstruction | creatinine; isoflurane; animal experiment; animal model; Article; chronic kidney failure; controlled study; creatinine blood level; disease exacerbation; feasibility study; fluorescence imaging; kidney weight; micro-computed tomography; mouse; multimodal imaging; nonhuman; optical coherence microscopy; photoacoustic microscopy; quantitative analysis; ureter obstruction | English | 2024 | 2024 (JUL-SEP) | 10.1016/j.bbe.2024.09.001 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Discovery of Spirosnuolides A-D, Type I/III Hybrid Polyketide Spiro-Macrolides for a Chemotherapeutic Lead against Lung Cancer | Four new macrolides, spirosnuolides A-D (1-4, respectively), were discovered from the termite nest-derived Kitasatospora sp. INHA29. Spirosnuolides A-D are 18-membered macrolides sharing an embedded [6,6]-spiroketal functionality inside the macrocycle and are conjugated with structurally uncommon side chains featuring cyclopentenone, 1,4-benzoquinone, hydroxyfuroic acid, or butenolide moieties. Structure elucidation was achieved using a combination of spectroscopic analyses, multiple chemical derivatizations (methylation, methanolysis, Luche reduction, and Mosher's reaction), X-ray diffraction analysis, and computational ECD calculations. Interestingly, genome sequencing analysis suggests that spirosnuolides were biosynthesized through a rare type I/III hybrid polyketide synthase. Importantly, spirosnuolide B displayed potent antiproliferative effects against various cancer cell lines at nanomolar concentrations, particularly against HCC827 cells, an EGFR mutant non-small-cell lung cancer (NSCLC) cell line, with a high safety index value. Based on in vitro studies, the antiproliferative mechanism of spirosnuolide B involved the activation of AMPK signaling, leading to cell cycle arrest and apoptosis in HCC827 cells. Its potent efficacy was also proven in vivo by the effective inhibition of tumor growth in mouse xenograft studies. Moreover, cotreatment with spirosnuolide B and gefitinib, synergistically enhanced the antiproliferative activity and apoptosis, suggesting a potential strategy to overcome gefitinib resistance in EGFR mutant NSCLC. | Huynh, Thanh-Hau; Jang, Sung Chul; Ban, Yeon Hee; Lee, Eun-Young; Kim, Taeho; Kang, Ilnam; An, Joon Soo; Kang, Sangwook; Han, Jaeho; Kwon, Yun; Oh, Daehyun; Park, Hyeung-geun; Cho, Jang-Cheon; Jang, Jichan; Oh, Ki-Bong; Nam, Sang-Jip; Lee, Sang Kook; Oh, Dong-Chan | Seoul Natl Univ, Nat Prod Res Inst, Coll Pharm, Seoul 08826, South Korea; Kangwon Natl Univ, Coll Biomed Sci, Dept Mol Biosci, Chunchon 24341, South Korea; Ewha Womans Univ, Dept Chem & Nanosci, Seoul 03760, South Korea; Gyeongsang Natl Univ, Res Inst Life Sci, Dept Bio & Med Big Data, Four Program BK21,Div Life Sci, Jinju 52828, South Korea; Inha Univ, Dept Biol Sci, Incheon 22212, South Korea; Kyungpook Natl Univ, Res Inst Pharmaceut Sci, Coll Pharm, Daegu 41566, South Korea; Seoul Natl Univ, Res Inst Pharmaceut Sci, Seoul 08826, South Korea; Seoul Natl Univ, Coll Pharm, Seoul 08826, South Korea; Seoul Natl Univ, Coll Agr & Life Sci, Dept Agr Biotechnol, Seoul 08826, South Korea; Seoul Natl Univ, Nat Prod Res Inst, Seoul 08826, South Korea | Cho, Jang-Cheon/B-4676-2013; Kim, Taeho/KSL-8430-2024; An, Joon Soo/NOF-1416-2025 | 57390777800; 57554826900; 35279095000; 57049722200; 57211534553; 7203062761; 57208526859; 57827837800; 57223649995; 56156932300; 57751591200; 7601570309; 7403534470; 24767832200; 7402730322; 57208839798; 59475456900; 8707854600 | sklee61@snu.ac.kr;dongchanoh@snu.ac.kr; | JACS AU | JACS AU | 2691-3704 | 4 | 12 | ESCI | CHEMISTRY, MULTIDISCIPLINARY | 2024 | 8.7 | 15.7 | 0.8 | 2025-05-07 | 3 | 3 | spiroketal macrolides; typeI/III polyketide synthase; lung cancer; AMPK signaling; antitumor efficacy | ACTIVATION; AMPK; BIOSYNTHESIS; COMBINATION; INHIBITION; GEFITINIB | AMPK signaling; antitumor efficacy; lung cancer; spiroketal macrolides; type I/III polyketide synthase | English | 2024 | 2024-12-10 | 10.1021/jacsau.4c00803 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Lightweight beat score map method for electrocardiogram-based arrhythmia classification | We recently investigated beat score map (BSM)-based methods for electrocardiogram (ECG)-based arrhythmia classification. Although BSM-based methods show impressive performance, they are somewhat resourceintensive owing to the arrangement of beat score vectors generated from 1D ECG sequences with zeropadding across time points. To address this issue, we propose a lightweight BSM (Lw-BSM) method that significantly reduces the size of the original BSM while capturing the characteristics of beat arrangement patterns as does the original BSM. Specifically, two types of Lw-BSMs are generated without zero-padding and evaluated for multiclass arrhythmia prediction. Experimental results on two public datasets, MIT-BIH and SPH, demonstrate that arrhythmia classification using Lw-BSM images is quite comparable to that using the original BSM images as an input to CNN-based classification models. At the same time, the image size can be reduced significantly. Moreover, it is observed that this approach is almost insensitive to the selection of the R-peak detection algorithm, showing stable performance across different R-peak algorithms. | Lee, Kyeonghwan; Lee, Jaewon; Shin, Miyoung | Kyungpook Natl Univ, Sch Elect & Elect Engn, Biointelligence & Data Min Lab, Daegu 41566, South Korea | 59421719800; 58377059800; 7401536642 | shinmy@knu.ac.kr; | BIOCYBERNETICS AND BIOMEDICAL ENGINEERING | BIOCYBERN BIOMED ENG | 0208-5216 | 44 | 4 | SCIE | ENGINEERING, BIOMEDICAL | 2024 | 6.6 | 15.7 | 0 | 2025-05-07 | 2 | 1 | Arrhythmia classification; Electrocardiogram; Beat score map; Lightweight method | NETWORK | Arrhythmia classification; Beat score map; Electrocardiogram; Lightweight method | Article; atrial fibrillation; atrioventricular nodal reentry tachycardia; continuous wavelet transform; controlled study; convolutional neural network; detection algorithm; discrete wavelet transform; electrocardiogram; heart arrhythmia; heart atrium flutter; heart left bundle branch block; heart ventricle extrasystole; human; image analysis; major clinical study; metaheuristics; RR interval; sinus bradycardia; sinus rhythm; sinus tachycardia; supraventricular premature beat; supraventricular tachycardia | English | 2024 | 2024 (OCT-DEC) | 10.1016/j.bbe.2024.11.002 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | A global re-analysis of regionally resolved emissions and atmospheric mole fractions of SF6 for the period 2005-2021 | We determine the global emission distribution of the potent greenhouse gas sulfur hexafluoride (SF6) for the period 2005-2021 using inverse modelling. The inversion is based on 50 d backward simulations with the Lagrangian particle dispersion model (LPDM) FLEXPART and on a comprehensive observation data set of SF6 mole fractions in which we combine continuous with flask measurements sampled at fixed surface locations and observations from aircraft and ship campaigns. We use a global-distribution-based (GDB) approach to determine baseline mole fractions directly from global SF6 mole fraction fields at the termination points of the backward trajectories. We compute these fields by performing an atmospheric SF6 re-analysis, assimilating global SF6 observations into modelled global three-dimensional mole fraction fields. Our inversion results are in excellent agreement with several regional inversion studies in the USA, Europe, and China. We find that (1) annual US SF6 emissions strongly decreased from 1.25 Gg in 2005 to 0.48 Gg in 2021; however, they were on average twice as high as the reported emissions to the United Nations. (2) SF6 emissions from EU countries show an average decreasing trend of -0.006 Gg yr-1 during the period 2005 to 2021, including a substantial drop in 2018. This drop is likely a direct result of the EU's F-gas regulation 517/2014, which bans the use of SF6 for recycling magnesium die-casting alloys as of 2018 and requires leak detection systems for electrical switch gear. (3) Chinese annual emissions grew from 1.28 Gg in 2005 to 5.16 Gg in 2021, with a trend of 0.21 Gg yr-1, which is even higher than the average global total emission trend of 0.20 Gg yr-1. (4) National reports for the USA, Europe, and China all underestimated their SF6 emissions. (5) Our results indicate increasing emissions in poorly monitored areas (e.g. India, Africa, and South America); however, these results are uncertain due to weak observational constraints, highlighting the need for enhanced monitoring in these areas. (6) Global total SF6 emissions are comparable to estimates in previous studies but are sensitive to a priori estimates due to the low network sensitivity in poorly monitored regions. (7) Monthly inversions indicate that SF6 emissions in the Northern Hemisphere were on average higher in summer than in winter throughout the study period. | Vojta, Martin; Plach, Andreas; Annadate, Saurabh; Park, Sunyoung; Lee, Gawon; Purohit, Pallav; Lindl, Florian; Lan, Xin; Muehle, Jens; Thompson, Rona L.; Stohl, Andreas | Univ Vienna, Dept Meteorol & Geophys, Vienna, Austria; Univ Urbino Carlo Bo, Dept Pure & Appl Sci, Urbino, Italy; Univ Sch Adv Studies IUSS, Dept Sci Technol & Soc, I-27100 Pavia, Italy; Kyungpook Natl Univ, Sch Earth Syst Sci, Dept Oceanog, Daegu, South Korea; NILU, Kjeller, Norway; Int Inst Appl Syst Anal IIASA, Laxenburg, Austria; Univ Calif San Diego, Scripps Inst Oceanog, San Diego, CA 92037 USA; NOAA, Global Monitoring Lab, Boulder, CO USA | ; Stohl, Andreas/A-7535-2008; LAN, XIN/AAX-4357-2020; Annadate, Saurabh/NDS-8353-2025; Plach, Andreas/HOH-3054-2023; Purohit, Pallav/I-3938-2019 | 57994738600; 56725800500; 57224807122; 57085459500; 59426705400; 53869452300; 58288279800; 55801282300; 55917306500; 55482250100; 7006107059 | martin.vojta@univie.ac.at; | ATMOSPHERIC CHEMISTRY AND PHYSICS | ATMOS CHEM PHYS | 1680-7316 | 1680-7324 | 24 | 21 | SCIE | ENVIRONMENTAL SCIENCES;METEOROLOGY & ATMOSPHERIC SCIENCES | 2024 | 5.1 | 15.8 | 0 | 2025-05-07 | 1 | 1 | SULFUR-HEXAFLUORIDE SF6; GREENHOUSE GASES; INVERSION; LIFETIMES; ABATEMENT; HISTORY; TRACER | Africa; China; Europe; India; South America; United States; airborne survey; atmospheric pollution; computer simulation; emission inventory; greenhouse gas; Lagrangian analysis; measurement method; Northern Hemisphere; numerical model; shipborne measurement; sulfur; three-dimensional modeling | English | 2024 | 2024-11-11 | 10.5194/acp-24-12465-2024 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Akkermansia muciniphila extracellular vesicles have a protective effect against hypertension | Akkermansia muciniphila (Am) shows a beneficial role as a probiotic in the treatment of metabolic syndrome. However, the mechanism remains to be elucidated. We tested the hypothesis that Am extracellular vesicles (AmEVs) have a protective effect against hypertension. Extracellular vesicles purified from anaerobically cultured Am were characterized by nanoparticle tracking analysis, transmission electron microscopy, and silver stain after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). AmEVs (1.0 x 10(10 )log particles/L) or vehicles were added into organ baths to induce vasorelaxation. In addition, AmEVs (1.0 x 10(8) or 1.0 x 10(9) particles/kg) or vehicles were injected into the tail veins of Wistar-Kyoto rats (WKYs) and spontaneously hypertensive rats (SHRs) weekly for 4 weeks. Peripheral blood mononuclear cells (PBMCs) and splenocytes isolated from both rat strains were analyzed by flow cytometry, RT-qPCR, and western blot. AmEVs affected neither vascular contraction nor endothelial relaxation in thoracic aortas. Moreover, AmEVs protected against the development of hypertension in SHRs without a serious adverse reaction. Additionally, AmEVs increased the population of T regulatory (Treg) cells and tended to reduce proinflammatory cytokines. These results indicate that AmEVs have a protective effect against hypertension without a serious adverse reaction. Therefore, it is foreseen that AmEVs may be utilized as a novel therapeutic for the treatment of hypertension. | Kim, Jee Young; Kim, Cheong-Wun; Oh, Su Young; Jang, Sungmin; Yetunde, Olarinoye Zainab; Kim, Bo A.; Hong, Su-Hyung; Kim, Inkyeom | Kyungpook Natl Univ, Dept Pharmacol, Daegu 41944, South Korea; Kyungpook Natl Univ, Cardiovasc Res Inst, Daegu 41944, South Korea; Kyungpook Natl Univ, BK21 Plus KNU Biomed Convergence Program, Daegu 41944, South Korea; Kyungpook Natl Univ, Sch Med, Dept Biomed Sci, Daegu 41944, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Microbiol & Immunol, Daegu 41940, South Korea | 57222261625; 56662531400; 57204016703; 57897824500; 58945602400; 58944578100; 8691449100; 7404144630 | hongsu@knu.ac.kr;inkim@knu.ac.kr; | HYPERTENSION RESEARCH | HYPERTENS RES | 0916-9636 | 1348-4214 | 47 | 6 | SCIE | PERIPHERAL VASCULAR DISEASE | 2024 | 4.6 | 15.8 | 4.1 | 2025-05-07 | 12 | 11 | Akkermansia muciniphila; Hypertension; Extracellular vesicles; Treg cells; Spontaneous hypertensive rats | REGULATORY T-CELLS | Akkermansia muciniphila; Extracellular vesicles; Hypertension; Spontaneous hypertensive rats; Treg cells | Akkermansia; Akkermansia muciniphila; Animals; Aorta, Thoracic; Blood Pressure; Extracellular Vesicles; Hypertension; Leukocytes, Mononuclear; Male; Probiotics; Rats; Rats, Inbred SHR; Rats, Inbred WKY; Spleen; Vasodilation; acetylcholine; allophycocyanin; interleukin 1beta; interleukin 6; phenylephrine; probiotic agent; Akkermansia muciniphila; Akkermansia muciniphila extracellular vesicle; animal experiment; animal model; animal tissue; Article; blood vessel reactivity; body weight; enzyme linked immunosorbent assay; exosome; flow cytometry; hypertension; immunoblotting; mononuclear cell; nonhuman; polyacrylamide gel electrophoresis; protective effect; protein expression; rat; reverse transcription polymerase chain reaction; transmission electron microscopy; ultracentrifugation; Western blotting; Akkermansia; Akkermansia muciniphila; animal; blood pressure; male; spleen; spontaneously hypertensive rat; thoracic aorta; vasodilatation; Wistar Kyoto rat | English | 2024 | 2024-06 | 10.1038/s41440-024-01627-5 | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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