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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | ○ | Article | Recent changes in heatwave characteristics over Korea | Global warming and abnormal climate change have resulted in an increase in the frequency of severe heatwave events. Recently, a series of extreme heatwave events have occurred in South Korea, and the damage from these events has also been increasing. Thus, it is necessary to analyze the mechanisms for generating and developing heatwaves. In this study, the long-term trend for heatwave events in South Korea was investigated using cluster analysis. Heatwave events in a 38-year period in South Korea were defined, and their synoptic patterns were categorized into three clusters. The number of heatwave days of cluster 2, which is related to the anomalous positive geopotential height (GPH) over the Kamchatka Peninsula, was found to significantly increase in recent years (2000-2018) compared with the past (1981-1999). In contrast, the frequency of cluster 3 associated with a negative GPH anomaly over the Kamchatka Peninsula decreased in the same period. There were five regions, including northern China and the Kamchatka Peninsula, where the mid-level GPH significantly increased between 2000 and 2018. This change in GPH was positively (negatively) correlated with the patterns associated to long-term variability of heatwave days of cluster 2 (cluster 3). The long-term trends of the GPH anomalies over five regions showed a significant correlation with the North Atlantic Oscillation (NAO) index during midsummer. As a result, it is likely that the heatwave events related to cluster 2 (cluster 3) have increased (decreased) in South Korea because the long-term variability of the summer NAO has recently induced a favorable (unfavorable) atmospheric condition for cluster 2 (cluster 3). | Yoon, Donghyuck; Cha, Dong-Hyun; Lee, Myong-In; Min, Ki-Hong; Kim, Joowan; Jun, Sang-Yoon; Choi, Yonghan | Ulsan Natl Inst Sci & Technol, Sch Urban & Environm Engn, Ulsan 44919, South Korea; Kyungpook Natl Univ, Dept Astron & Atmospher Sci, Daegu 41566, South Korea; Kongju Natl Univ, Dept Atmospher Sci, Gongju, South Korea; Korea Polar Res Inst, Unit Arctic Sea Ice Predict, Incheon 21990, South Korea | ; Lee, Myong-In/F-3578-2010; Yoon, Donghyuck/ISS-5217-2023; Cha, Dong-Hyun/F-4901-2015 | 57204630556; 55418553100; 58530782000; 37089364100; 36637539100; 43561261500; 54789553800 | dhcha@unist.ac.kr; | CLIMATE DYNAMICS | CLIM DYNAM | 0930-7575 | 1432-0894 | 55 | 7-8 | SCIE | METEOROLOGY & ATMOSPHERIC SCIENCES | 2020 | 4.375 | 22.9 | 1.8 | 2025-06-25 | 41 | 42 | Heat wave; Cluster analysis; Long-term variability; North Atlantic Oscillation; South Korea | SUMMER HEAT; WAVES; IDENTIFICATION; PRECIPITATION; VARIABILITY; MORTALITY; IMPACT; RISK | Cluster analysis; Heat wave; Long-term variability; North Atlantic Oscillation; South Korea | Kamchatka; Kamchatka Peninsula; Russian Federation; South Korea; climate change; geopotential; global warming; heat wave; North Atlantic Oscillation | English | 2020 | 2020-10 | 10.1007/s00382-020-05420-1 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Reference gene selection for qRT-PCR analysis of season- and tissue-specific gene expression profiles in the honey bee Apis mellifera | Honey bees are both important pollinators and model insects due to their highly developed sociality and colony management. To better understand the molecular mechanisms underlying honey bee colony management, it is important to investigate the expression of genes putatively involved in colony physiology. Although quantitative real-time PCR (qRT-PCR) can be used to quantify the relative expression of target genes, internal reference genes (which are stably expressed across different conditions) must first be identified to ensure accurate normalisation of target genes. To identify reliable reference genes in honey bee (Apis mellifera) colonies, therefore, we evaluated seven candidate genes (ACT, EIF, EF1, RPN2, RPS5, RPS18 and GAPDH) in samples collected from three honey bee tissue types (head, thorax and abdomen) across all four seasons using three analysis programmes (NormFinder, BestKeeper and geNorm). Subsequently, we validated various normalisation methods using each of the seven reference genes and a combination of multiple genes by calculating the expression of catalase (CAT). Although the genes ranked as the most stable gene were slightly different on conditions and analysis methods, our results suggest that RPS5, RPS18 and GAPDH represent optimal honey bee reference genes for target gene normalisation in qRT-PCR analysis of various honey bee tissue samples collected across seasons. | Jeon, Ji Hyang; Moon, KyungHwan; Kim, YeongHo; Kim, Young Ho | Kyungpook Natl Univ, Dept Appl Biol, Sangju, South Korea; Kyungpook Natl Univ, Dept Ecol Sci, Sangju, South Korea | Kim, Young/J-5414-2012 | 57218550812; 57202874378; 57204608118; 58516491300 | yhkim05@knu.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 10 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2020 | 4.38 | 22.9 | 1.22 | 2025-06-25 | 46 | 47 | REAL-TIME PCR; POLYMERASE-CHAIN-REACTION; DROSOPHILA-MELANOGASTER; JUVENILE-HORMONE; VITELLOGENIN; VALIDATION; STABILITY; TITERS; GLAND | Animals; Bees; DNA Primers; Gene Expression; Gene Expression Profiling; Organ Specificity; Pupa; Real-Time Polymerase Chain Reaction; Reference Standards; Transcriptome; primer DNA; transcriptome; animal; antibody specificity; bee; gene expression; gene expression profiling; genetics; pupa; real time polymerase chain reaction; standard | English | 2020 | 2020-08-18 | 10.1038/s41598-020-70965-4 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Regulation of Ras homolog family member G by microRNA-124 regulates proliferation and migration of human retinal pigment epithelial cells | Uncontrolled retinal pigment epithelial (RPE) cell proliferation/migration contribute to the pathological tractional membrane development in proliferative vitreoretinopathy. Recent studies reported that microRNA (miR)-124 controls various cellular functions via the direct targeting of small Ras homolog family member G (RHOG). Therefore, we investigated the role of the neuron-specific miR-124 and RHOG in RPE cell proliferation/migration. Alterations in miR-124 and RhoG expression, as per cell confluence were evaluated through quantitative real-time PCR and western blotting, respectively. After transfection with miR-124, we quantified RPE cell viability and migration and observed cell polarization and lamellipodia protrusions. We evaluated the expression of RHOG/RAC1 pathway molecules in miR-124-transfected RPE cells. Endogenous miR-124 expression increased proportionally to RPE cell density, but decreased after 100% confluence. Overexpression of miR-124 decreased cell viability and migration, BrdU incorporation, and Ki-67 expression. Inhibition of endogenous miR-124 expression promoted RPE cell migration. Transfection with miR-124 reduced cell polarization, lamellipodia protrusion, and RHOG mRNA 3 ' untranslated region luciferase activity. Like miR-124 overexpression, RhoG knockdown decreased RPE cell viability, wound healing, and migration, and altered the expression of cell cycle regulators. These results suggest that miR-124 could be a therapeutic target to alleviate fibrovascular proliferation in retinal diseases by regulating RPE proliferation/migration via RHOG. | Jun, Jong Hwa; Son, Myeong-Jin; Lee, Hyun-Gyo; Shim, Kyu Young; Baek, Won-Ki; Kim, Jae-Young; Joo, Choun-Ki | Keimyung Univ, Dept Ophthalmol, Dongsan Med Ctr, Sch Med, Daegu, South Korea; Keimyung Univ, Dept Microbiol, Sch Med, Daegu, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Oral Biochem, IHBR, Daegu, South Korea; Catholic Univ Korea, Seoul St Marys Hosp, Dept Ophthalmol & Visual Sci, Coll Med, Seoul, South Korea | Lee, Hyo/G-6299-2019; Kim, Ji-Youn/A-5779-2017 | 25643855300; 57211849351; 57192499227; 57219112115; 7005596145; 56812734700; 57223638638 | junjonghwa@gmail.com; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 10 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2020 | 4.38 | 22.9 | 0.18 | 2025-06-25 | 3 | 5 | MESENCHYMAL TRANSITION; CONTACT INHIBITION; RHOG; VITREORETINOPATHY; DIFFERENTIATION; IDENTIFICATION; NEUROGENESIS; DEBRIDEMENT; EXPRESSION; RAC1 | Cell Movement; Cell Proliferation; Cells, Cultured; Down-Regulation; Epithelial Cells; Humans; MicroRNAs; Neurons; Retinal Pigment Epithelium; Retinal Pigments; rho GTP-Binding Proteins; RNA, Messenger; Vitreoretinopathy, Proliferative; messenger RNA; microRNA; MIRN124 microRNA, human; Rho guanine nucleotide binding protein; RHOG protein, human; visual pigment; cell culture; cell motion; cell proliferation; down regulation; epithelium cell; genetics; human; nerve cell; pathology; retinal pigment epithelium; vitreoretinopathy | English | 2020 | 2020-09-22 | 10.1038/s41598-020-72360-5 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Relationship between the actual fine dust concentration and media exposure that influenced the changes in outdoor activity behavior in South Korea | The one reason of the decrease of walking time for adults in South Korea among various factors is the sense of fear about fine dust sparked by media reports, which has created a negative perception of fine dust. This study aimed to assess the change in concentration of fine dust, as well as individuals' walking time and health status, in South Korea, and to investigate the relationship between the media reports on fine dust. Using the national government statistics data, we analyzed the relationship between walking time, concentration of fine dust, and amount of media reports on fine dust. From 2008 to 2017, the average walking time and PM10 levels decreased from 76.17 to 49.47 min and 52 to 45 mu g/m(3); whereas PM10 media frequency increased from 349 to 9,234. No positive correlation existed between walking time in South Korea and exposure to fine dust. However, media reports on fine dust increased steadily from 2012 and peaked in 2015. The decrease in average walking time in South Korea was due to the negative perception created by the increase in media reports on fine dust, rather than the increase in the actual concentration of fine dust. | Kim, Myung-Gwan; Lee, Su-Jin; Park, Donghwi; Kim, Chul-hyun; Lee, Ki-hoon; Hwang, Jong-moon | Kyungpook Natl Univ, Grad Sch Publ Hlth, Daegu, South Korea; Ulsan Univ Hosp, Dept Phys Med & Rehabil, Ulsan, South Korea; Univ Ulsan, Coll Med, Ulsan, South Korea; Kyungpook Natl Univ Hosp, Dept Rehabil Med, 200 Dongduk Ro, Daegu 700721, South Korea; Kyungpook Natl Univ, Sch Med, Dept Rehabil Med, 200 Dongduk Ro, Daegu 700721, South Korea; Mompyeonhan Rehabil Clin, Daegu, South Korea | Park, Donghwi/GYQ-6185-2022 | 57217987383; 57207933350; 56606561400; 59603554500; 57218161131; 56367634000 | hti82@hanmail.net; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 10 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2020 | 4.38 | 22.9 | 0.59 | 2025-06-25 | 21 | 20 | PARTICULATE MATTER; AIR-POLLUTION; HEALTH; IMPACT; PM2.5; RISK; WALKING; CRISIS; STATE | Adult; Aged; Behavior; Dust; Environmental Monitoring; Female; Health Expenditures; Humans; Male; Middle Aged; Nutrition Surveys; Particulate Matter; Republic of Korea; Statistics as Topic; Time Factors; Walking; Young Adult; adult; aged; behavior; dust; environmental monitoring; female; health care cost; human; male; middle aged; nutrition; particulate matter; South Korea; statistics; time factor; walking; young adult | English | 2020 | 2020-07-20 | 10.1038/s41598-020-68580-4 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Risk prediction for malignant intraductal papillary mucinous neoplasm of the pancreas: logistic regression versus machine learning | Most models for predicting malignant pancreatic intraductal papillary mucinous neoplasms were developed based on logistic regression (LR) analysis. Our study aimed to develop risk prediction models using machine learning (ML) and LR techniques and compare their performances. This was a multinational, multi-institutional, retrospective study. Clinical variables including age, sex, main duct diameter, cyst size, mural nodule, and tumour location were factors considered for model development (MD). After the division into a MD set and a test set (2:1), the best ML and LR models were developed by training with the MD set using a tenfold cross validation. The test area under the receiver operating curves (AUCs) of the two models were calculated using an independent test set. A total of 3,708 patients were included. The stacked ensemble algorithm in the ML model and variable combinations containing all variables in the LR model were the most chosen during 200 repetitions. After 200 repetitions, the mean AUCs of the ML and LR models were comparable (0.725 vs. 0.725). The performances of the ML and LR models were comparable. The LR model was more practical than ML counterpart, because of its convenience in clinical use and simple interpretability. | Kang, Jae Seung; Lee, Chanhee; Song, Wookyeong; Choo, Wonho; Lee, Seungyeoun; Lee, Sungyoung; Han, Youngmin; Bassi, Claudio; Salvia, Roberto; Marchegiani, Giovanni; Wolfgang, Cristopher L.; He, Jin; Blair, Alex B.; Kluger, Michael D.; Su, Gloria H.; Kim, Song Cheol; Song, Ki-Byung; Yamamoto, Masakazu; Higuchi, Ryota; Hatori, Takashi; Yang, Ching-Yao; Yamaue, Hiroki; Hirono, Seiko; Satoi, Sohei; Fujii, Tsutomu; Hirano, Satoshi; Lou, Wenhui; Hashimoto, Yasushi; Shimizu, Yasuhiro; Del Chiaro, Marco; Valente, Roberto; Lohr, Matthias; Choi, Dong Wook; Choi, Seong Ho; Heo, Jin Seok; Motoi, Fuyuhiko; Matsumoto, Ippei; Lee, Woo Jung; Kang, Chang Moo; Shyr, Yi-Ming; Wang, Shin-E; Han, Ho-Seong; Yoon, Yoo-Seok; Besselink, Marc G.; van Huijgevoort, Nadine C. M.; Sho, Masayuki; Nagano, Hiroaki; Kim, Sang Geol; Honda, Goro; Yang, Yinmo; Yu, Hee Chul; Do Yang, Jae; Chung, Jun Chul; Nagakawa, Yuichi; Seo, Hyung Il; Choi, Yoo Jin; Byun, Yoonhyeong; Kim, Hongbeom; Kwon, Wooil; Park, Taesung; Jang, Jin-Young | Seoul Natl Univ, Dept Surg, Coll Med, 101 Daehak Ro, Seoul 03080, South Korea; Seoul Natl Univ, Canc Res Inst, Coll Med, 101 Daehak Ro, Seoul 03080, South Korea; Seoul Natl Univ, Dept Stat, 56-1 Shill Dong, Seoul 151747, South Korea; Seoul Natl Univ, Interdisciplinary Program Biostat, 56-1 Shill Dong, Seoul 151747, South Korea; Sejong Univ, Dept Math & Stat, Seoul, South Korea; Seoul Natl Univ Hosp, Ctr Precis Med, Seoul, South Korea; Univ Verona Hosp Trust, Pancreas Inst, Dept Gen & Pancreat Surg, Verona, Italy; Johns Hopkins Univ, Sch Med, Dept Surg, Baltimore, MD 21205 USA; Columbia Univ, Coll Phys & Surg, Dept Surg, Div Gastrointestinal & Endocrine Surg, New York, NY USA; Columbia Univ, Dept Pathol & Cell Biol, Med Ctr, New York, NY USA; Univ Ulsan, Asan Med Ctr, Dept Surg, Coll Med, Seoul, South Korea; Tokyo Womens Med Univ, Inst Gastroenterol, Dept Surg, Tokyo, Japan; Int Univ Hlth & Welf, Dept Surg, Mita Hosp, Tokyo, Japan; Natl Taiwan Univ Hosp, Dept Surg, Taipei, Taiwan; Natl Taiwan Hosp, Taipei, Taiwan; Wakayama Med Univ, Sch Med, Dept Surg 2, Wakayama, Japan; Kansai Med Univ, Dept Surg, Osaka, Japan; Nagoya Univ, Grad Sch Med, Dept Gastroenterol Surg Surg 2, Nagoya, Aichi, Japan; Univ Toyama, Acad Assembly, Fac Med, Dept Surg & Sci, Toyama, Japan; Hokkaido Univ, Fac Med, Dept Gastroenterol Surg 2, Sapporo, Hokkaido, Japan; Fudan Univ, Zhongshan Hosp, Dept Pancreat Surg, Shanghai, Peoples R China; Hiroshima Univ, Inst Biomed & Hlth Sci, Dept Surg, Hiroshima, Japan; Hiroshima Mem Hosp, Dept Surg, Hiroshima, Japan; Aichi Canc Ctr Hosp, Gastroenterol Surg, Nagoya, Aichi, Japan; Karolinska Univ Hosp, Ctr Digest Dis, Karolinska Inst,Div Surg,Pancreat Surg Unit, Dept Clin Sci Intervent & Technol CLINTEC, Stockholm, Sweden; Univ Colorado, Dept Surg, Anschutz Med Campus, Denver, CO USA; Karolinska Inst, Dept Clin Sci Intervent & Technol CLINTEC, Stockholm, Sweden; Karolinska Univ Hosp, Dept Digest Dis, Stockholm, Sweden; Sungkyunkwan Univ, Dept Surg, Sch Med, Seoul, South Korea; Tohoku Univ, Dept Surg, Tohoku, Japan; Kobe Univ, Dept Surg, Grad Sch Med, Kobe, Hyogo, Japan; Kindai Univ, Fac Med, Dept Surg, Osaka, Japan; Yonsei Univ, Severance Hosp, Coll Med, Yonsei Canc Ctr,Pancreaticobiliary Canc Clin, Seoul, South Korea; Taipei Vet Gen Hosp, Dept Surg, Taipei, Taiwan; Natl Yang Ming Univ, Taipei, Taiwan; Seoul Natl Univ, Coll Med, Dept Surg, Bundang Hosp, Seoul, South Korea; Univ Amsterdam, Canc Ctr Amsterdam, Dept Surg, Amsterdam UMC, Amsterdam, Netherlands; Univ Amsterdam, Dept Gastroenterol & Hepatol, Amsterdam UMC, Amsterdam Gastroenterol Endocrinol Metab, Amsterdam, Netherlands; Nara Med Univ, Dept Surg, Nara, Japan; Osaka Univ, Dept Surg, Grad Sch Med, Osaka, Japan; Yamaguchi Univ, Gastroenterol Breast & Endocrine Surg, Yamaguchi, Japan; Kyungpook Natl Univ, Dept Surg, Daegu, South Korea; Komagome Hosp, Tokyo Metropolitan Canc & Infect Dis Ctr, Dept Surg, Tokyo, Japan; Peking Univ First Hosp, Dept Gen Surg, Beijing, Peoples R China; Jeonbuk Natl Univ, Dept Surg, Med Sch, Jeonju, South Korea; Soonchunhyang Univ, Dept Surg, Asan, South Korea; Tokyo Med Univ, Dept Gastrointestinal & Pediat Surg, Tokyo, Japan; Pusan Natl Univ, Dept Surg, Pusan, South Korea | Löhr, Matthias/A-3692-2012; Blair, Alex/K-8118-2016; Lee, Jae/AAX-7095-2020; Marchegiani, Giovanni/J-1823-2018; kim, jeeyoung/AFK-5620-2022; Yang, Ching/J-5173-2015; Salvia, Roberto/J-1773-2018; Yoon, Yoo-Seok/AAD-4820-2020; MATSUMOTO, IPPEI/AFK-9075-2022; Lee, Sungyoung/L-3433-2018; Fujii, Tsutomu/AAN-4890-2021; Yang, Xing/S-4994-2018; Besselink, Marc/R-4268-2019; Honda, Goro/AAZ-8447-2020; Del Chiaro, Marco/JUV-6733-2023; Higuchi, Ryota/AAF-4351-2020; Kang, Jae/J-5363-2012; Kim, Kyung/I-5501-2015; Su, Gloria/AAJ-1457-2020 | 22950347400; 57219941916; 57219938946; 58413683100; 15754275000; 55716390100; 57206914279; 7102974312; 6701399875; 57214806473; 15133694600; 55499312000; 57193685401; 36835277500; 57217534426; 55919519700; 37117816900; 58089030500; 7006399093; 7006442353; 56431462100; 35391343600; 10039695200; 6603919544; 35425692000; 7402888531; 59157651000; 7404551550; 57191051791; 55864601100; 57224772212; 55605216000; 9245957100; 24477199400; 7102832040; 6602471170; 7201948952; 7401752815; 34968209400; 35428646100; 9843531000; 7401969217; 7402126748; 6603166269; 57190002218; 55863591500; 57200379762; 21735842600; 7005333604; 57211734732; 7405855968; 41562471000; 14819232800; 36480327700; 26425277100; 57210900909; 57208165048; 57103962000; 35211119200; 34668299500; 7402965187 | tspark@stats.snu.ac.kr;jangjy4@snu.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 10 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2020 | 4.38 | 22.9 | 0.45 | 2025-06-25 | 13 | 13 | VALIDATION; GUIDELINES; NOMOGRAM | Aged; Algorithms; Diagnosis, Computer-Assisted; Female; Humans; Logistic Models; Machine Learning; Male; Middle Aged; Pancreatic Cyst; Pancreatic Intraductal Neoplasms; Prognosis; Retrospective Studies; Risk Factors; aged; algorithm; clinical trial; comparative study; computer assisted diagnosis; diagnostic imaging; female; human; machine learning; male; middle aged; multicenter study; pancreas cyst; pathology; procedures; prognosis; retrospective study; risk factor; statistical model | English | 2020 | 2020-11-18 | 10.1038/s41598-020-76974-7 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | RNA sequencing as an alternative tool for detecting measurable residual disease in core-binding factor acute myeloid leukemia | DNA sequencing-based measurable residual disease (MRD) detection has shown to be clinically relevant in AML. However, the same methodology cannot be applied to fusion gene-driven subtypes of AML such as core-binding factor AML (CBF-AML). Here in this study, we evaluated the effectiveness of using DNA and RNA sequencing in MRD detection and in tracking clonal dynamics in CBF-AML. Using RNA-seq, we were able to quantify expression levels of RUNX1-RUNX1T1 and CBFB-MYH11 at diagnosis and their levels of reduction during remission (P<6.3e-05 and P<2.2e-13). The level of reduction of RUNX1-RUNX1T1 as measured by RNA-seq and qPCR were highly correlated (R-2=0.74, P<5.4e-05). A decision tree analysis, based on 3-log reduction of RUNX1-RUNX1T1 and cKIT-D816(mut) at diagnosis, stratified RUNX1-RUNX1T1 AML patients into three subgroups. These three subgroups had 2-year overall survival rates at 87%, 74%, and 33% (P<0.08) and 2-year relapse incidence rates at 13%, 42%, and 67% (P<0.05). On the other hand, although low residual allelic burden was common, it was not associated with long-term outcome, indicating that mutation clearance alone cannot be interpreted as MRD-negative. Overall, our study demonstrates that the clinical utility of RNA sequencing as a potential tool for MRD monitoring in fusion gene-driven AML such as RUNX1-RUNX1T1 AML. | Kim, TaeHyung; Moon, Joon Ho; Ahn, Jae-Sook; Ahn, Seo-Yeon; Jung, Sung-Hoon; Yang, Deok-Hwan; Lee, Je-Jung; Shin, Myung-Geun; Choi, Seung Hyun; Lee, Ja-yeon; Tyndel, Marc S.; Lee, Hui Young; Kim, Kyoung Ha; Cai, Yu; Lee, Yoo Jin; Sohn, Sang Kyun; Min, Yoo Hong; Cheong, June-Won; Kim, Hyeoung-Joon; Zhang, Zhaolei; Kim, Dennis Dong Hwan | Univ Toronto, Dept Comp Sci, Toronto, ON, Canada; Univ Toronto, Donnelly Ctr Cellular & Biomol Res, Toronto, ON, Canada; Kyungpook Natl Univ Hosp, Dept Hematol Oncol, Daegu, South Korea; Chonnam Natl Univ, Hwasun Hosp, Dept Hematol Oncol, Hwasun, Jeollanam Do, South Korea; Chonnam Natl Univ, Hwasun Hosp, Coll Med, Genome Res Ctr Hematopoiet Dis, Hwasun Gun, Jeollanam Do, South Korea; Chonnam Natl Univ, Hwasun Hosp, Dept Lab Med, Hwasun, Jeollanam Do, South Korea; Univ Toronto, Edward S Rogers Sr Dept Elect & Comp Engn, Toronto, ON, Canada; Kangwon Natl Univ, Kangwon Natl Univ Hosp, Sch Med, Dept Internal Med, Chunchon, Gangwon Do, South Korea; Soonchunhyang Univ Hosp, Dept Internal Med, Seoul, South Korea; Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Hematol, Shanghai, Peoples R China; Yonsei Univ, Dept Internal Med, Seoul, South Korea; Univ Toronto, Dept Mol Genet, Toronto, ON, Canada; Princess Margaret Canc Ctr, Dept Med Oncol & Hematol, Toronto, ON, Canada | ; Kim, Dennis/AAH-8499-2019; Kim, Tae/O-4252-2015; Zhang, Zhaolei/G-2369-2017; Lee, Jung-Hye/F-6974-2013; Lee, Yong Jae/GLR-4153-2022 | 55763792349; 56568642700; 22984055900; 55945078500; 55511978300; 8701758000; 7601478211; 7401537550; 57203643100; 55859183300; 55173389000; 57193578200; 7409319096; 57217244829; 57188669696; 13310226800; 7202197132; 7004933294; 7410127473; 57203273189; 56312200900 | hjoonk@chonnam.ac.kr;zhaolei.zhang@utoronto.ca;dr.dennis.kim@uhn.ca; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 10 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2020 | 4.38 | 22.9 | 0.14 | 2025-06-25 | 8 | 6 | AML; MUTATIONS; PCR | Adolescent; Adult; Aged; Aged, 80 and over; Core Binding Factor Alpha 2 Subunit; Core Binding Factors; Female; Gene Expression Regulation, Leukemic; Gene Rearrangement; Humans; Leukemia, Myeloid, Acute; Male; Middle Aged; Mutation; Myosin Heavy Chains; Neoplasm, Residual; Oncogene Proteins, Fusion; Prognosis; Proof of Concept Study; RUNX1 Translocation Partner 1 Protein; Sequence Analysis, RNA; Young Adult; AML1-ETO fusion protein, human; core binding factor; MYH11 protein, human; myosin heavy chain; oncoprotein; protein CBFA2T1; RUNX1T1 protein, human; transcription factor RUNX1; acute myeloid leukemia; adolescent; adult; aged; female; gene expression regulation; gene rearrangement; genetics; human; male; middle aged; minimal residual disease; mortality; mutation; pathology; procedures; prognosis; proof of concept; sequence analysis; very elderly; young adult | English | 2020 | 2020-11-18 | 10.1038/s41598-020-76933-2 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Serial optical coherence microscopy for label-free volumetric histopathology | The observation of histopathology using optical microscope is an essential procedure for examination of tissue biopsies or surgically excised specimens in biological and clinical laboratories. However, slide-based microscopic pathology is not suitable for visualizing the large-scale tissue and native 3D organ structure due to its sampling limitation and shallow imaging depth. Here, we demonstrate serial optical coherence microscopy (SOCM) technique that offers label-free, high-throughput, and large-volume imaging of ex vivo mouse organs. A 3D histopathology of whole mouse brain and kidney including blood vessel structure is reconstructed by deep tissue optical imaging in serial sectioning techniques. Our results demonstrate that SOCM has unique advantages as it can visualize both native 3D structures and quantitative regional volume without introduction of any contrast agents. | Min, Eunjung; Ban, Sungbea; Lee, Junwon; Vavilin, Andrey; Baek, Songyee; Jung, Sunwoo; Ahn, Yujin; Park, Kibeom; Shin, Sungwon; Han, SoHyun; Cho, Hyungjoon; Lee-Kwon, Whaseon; Kim, Jeehyun; Lee, C. Justin; Jung, Woonggyu | Max Planck Inst Biol Cybernet, D-72076 Tubingen, Germany; Ulsan Natl Inst Sci & Technol UNIST, Dept Biomed Engn, Ulsan 44919, South Korea; Martinos Ctr Biomed Imaging, Charlestown, MA 02129 USA; Kyungpook Natl Univ, Sch Elect Engn, Daegu 41566, South Korea; Inst for Basic Sci Korea, Ctr Cognit & Social, Daejeon 34126, South Korea | Lee-Kwon, Whaseon/D-6021-2011; Lee, C./AAC-1663-2019; Park, Kibeom/ABE-3860-2020 | 57210986768; 57193730120; 57104668900; 23391316200; 57188556481; 57131805300; 57188564926; 56017776400; 57040259000; 53263948700; 56234328500; 6602100693; 7601373350; 7410148866; 58291338100 | giovanna.cilluffo@irib.cnr.it; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 10 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2020 | 4.38 | 22.9 | 0.36 | 2025-06-25 | 10 | 12 | SINGLE-CELL RESOLUTION; BRAIN; TOMOGRAPHY; TISSUES | Animals; Brain; Kidney; Magnetic Resonance Imaging; Male; Mice, Inbred C57BL; Microscopy; Staining and Labeling; Tomography, Optical Coherence; animal; brain; C57BL mouse; diagnostic imaging; kidney; male; microscopy; nuclear magnetic resonance imaging; optical coherence tomography; pathology; staining | English | 2020 | 2020-04-21 | 10.1038/s41598-020-63460-3 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Streptozotocin Induces Alzheimer's Disease-Like Pathology in Hippocampal Neuronal Cells via CDK5/Drp1-Mediated Mitochondrial Fragmentation | Aberrant brain insulin signaling plays a critical role in the pathology of Alzheimer's disease (AD). Mitochondrial dysfunction plays a role in the progression of AD, with excessive mitochondrial fission in the hippocampus being one of the pathological mechanisms of AD. However, the molecular mechanisms underlying the progression of AD and mitochondrial fragmentation induced by aberrant brain insulin signaling in the hippocampal neurons are poorly understood. Therefore, we investigated the molecular mechanistic signaling associated with mitochondrial dynamics using streptozotocin (STZ), a diabetogenic compound, in the hippocampus cell line, HT-22 cells. In this metabolic dysfunctional cellular model, hallmarks of AD such as neuronal apoptosis, synaptic loss, and tau hyper-phosphorylation are induced by STZ. We found that in the mitochondrial fission protein Drp1, phosphorylation is increased in STZ-treated HT-22 cells. We also determined that inhibition of mitochondrial fragmentation suppresses STZ-induced AD-like pathology. Furthermore, we found that phosphorylation of Drp1 was induced by CDK5, and inhibition of CDK5 suppresses STZ-induced mitochondrial fragmentation and AD-like pathology. Therefore, these findings indicate that mitochondrial morphology and functional regulation may be a strategy of potential therapeutic for treating abnormal metabolic functions associated with the pathogenesis of AD. | Park, Junghyung; Won, Jinyoung; Seo, Jincheol; Yeo, Hyeon-Gu; Kim, Keonwoo; Kim, Yu Gyeong; Jeon, Chang-Yeop; Kam, Min Kyoung; Kim, Young-Hyun; Huh, Jae-Won; Lee, Sang-Rae; Lee, Dong-Seok; Lee, Youngjeon | Korea Res Inst Biosci & Biotechnol KRIBB, Natl Primate Res Ctr, Cheongju, South Korea; Korea Univ Sci & Technol, KRIBB Sch Biosci, Dept Funct Genom, Daejeon, South Korea; Kyungpook Natl Univ, BK21 Plus KNU Creat BioRes Grp, Sch Life Sci, Daegu, South Korea | Lee, Youngjeon/LZH-8969-2025; 김, 영현/HLH-3847-2023; Park, Junghyung/KXQ-7522-2024 | 55671747100; 56018670200; 57200518242; 56263762800; 57204572034; 57219109450; 56522472100; 57195564169; 54393408600; 16645802900; 16026266200; 57210068061; 57199022088 | neurosci@kribb.re.kr; | FRONTIERS IN CELLULAR NEUROSCIENCE | FRONT CELL NEUROSCI | 1662-5102 | 14 | SCIE | NEUROSCIENCES | 2020 | 5.505 | 22.9 | 1.02 | 2025-06-25 | 34 | 34 | Alzheimer's disease (AD); streptozotocin (STZ); hippocampus; mitochondrial dynamics; dynamin-1-like protein (Drp1); cyclin-dependent kinase 5 (CDK5) | CYCLIN-DEPENDENT KINASE-5; AMYLOID-BETA; OXIDATIVE STRESS; DIFFERENTIAL MODULATION; INDUCED NEUROTOXICITY; INSULIN-RESISTANCE; IN-VIVO; FISSION; MODEL; DYNAMICS | Alzheimer’s disease (AD); cyclin-dependent kinase 5 (CDK5); dynamin-1-like protein (Drp1); hippocampus; mitochondrial dynamics; streptozotocin (STZ) | English | 2020 | 2020-08-04 | 10.3389/fncel.2020.00235 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Structural dynamics of CH3NH3⁺ and PbBr3⁻ in tetragonal and cubic phases of CH3NH3PbBr3 hybrid perovskite by nuclear magnetic resonance | Understanding the structural dynamics of lead-halide perovskites is essential for their advanced use as photovoltaics. Here, the structural dynamics of the CH3NH3 cation and PbBr6 octahedra in the perovskite CH3NH3PbBr3 were studied via nuclear magnetic resonance (NMR) to determine the mechanism of the transition from the tetragonal to cubic phase. The chemical shifts were obtained by H-1, C-13, and Pb-207 magic angle spinning NMR and N-14 static NMR. The chemical shifts of the H-1 nuclei in CH3 and NH3 remained constant with increasing temperature, whereas those of the C-13 and Pb-207 nuclei varied near the phase transition temperature (T-C=236 K), indicating that the structural environments of C-13 and Pb-207 change near T-C. The spin-lattice relaxation time T-1 rho values for H-1, C-13, and Pb-207 nuclei increased with increasing temperature and did not exhibit an abrupt change near T-C. In addition, the two lines in the N-14 NMR spectra superposed into one line near T-C, indicating the occurrence of a phase transition to a cubic phase with higher symmetry than tetragonal. Consequently, the main factor causing the phase transition from the tetragonal to cubic phase near T-C is a change in the surroundings of the Pb-207 nuclei in the PbBr6 octahedra and of the C-N groups in the CH3NH3 cations. | Lim, Ae Ran; Kim, Sun Ha; Joo, Yong Lak | Jeonju Univ, Analyt Lab Adv Ferroelect Crystals, Dept Sci Educ, Jeonju 55069, South Korea; Korea Basic Sci Inst, Seoul Western Ctr, Seoul 03759, South Korea; Kyungpook Natl Univ, Dept Chem, Daegu 41566, South Korea; Cornell Univ, Robert Fredrick Smith Sch Chem & Biomol Engn, Ithaca, NY 14853 USA | Joo, Yong/AAA-8616-2019 | 7202659025; 54386953600; 7102315465 | aeranlim@hanmail.net; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 10 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2020 | 4.38 | 22.9 | 0.32 | 2025-06-25 | 7 | 8 | SENSITIZED SOLAR-CELL; METHYLAMMONIUM; TRANSITIONS; CATION; EFFICIENCY; NMR | English | 2020 | 2020-08-04 | 10.1038/s41598-020-70128-5 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||||
| ○ | ○ | Article | The effectiveness of nonsteroidal anti-inflammatory drugs and acetaminophen in reduce the risk of amyotrophic lateral sclerosis? A meta-analysis | To test the hypothesis that aspirin, non-aspirin nonsteroidal anti-infammatory drugs (NA-NSAIDs), or acetaminophen can reduce the risk of ALS, we conducted a systematic review and meta-analysis of related previous studies. A comprehensive search was conducted on the PubMed, Embase, Cochrane Library and SCOPUS databases. It included studies published up to 29 February 2020 that fulfilled our inclusion criteria. Aspirin, acetaminophen and NA-NSAIDs use information, between the ALS and control groups, was collected for the meta-analysis. Rates of aspirin, NA-NSAID, and acetaminophen use in ALS group, compared with control group were investigated. In the results, only three studies that relate the risk of ALS to aspirin, NA-NSAIDs and acetaminophen use satisfied the inclusion criteria for the meta-analysis. Regarding aspirin, the studies did not show any statistically significant difference in aspirin use between the ALS and control groups (Odds ratio, 1.04 [95% confidence interval, 0.90-1.21]). NA-NSAIDs and acetaminophen use, however, did show up statistically significant differences in between the ALS and control groups. (Odds ratio, 0.82 [95% confidence interval, 0.73-0.91]) and (Odds ratio, 0.80 [95% confidence interval, 0.69-0.93]). However, our study has some limitations. Firstly, we only included a small number of studies. Secondly, the included studies did not control for past medical history, which may have confounded their results, and in turn, could have caused bias in our study. Thirdly, in this meta-analysis, the ALS patients were not subdivided into sporadic or familial type. Lastly, the studies also did not consider the types of NSAIDs and dosages used of each drug. For more convincing evidence regarding the effectiveness of aspirin, NA-NSAIDs and acetaminophen to reduce the risk of ALS occurrence, more qualified prospective studies are required. In conclusion, the use of NA-NSAIDs and acetaminophen is associated with a decreased risk for the development of ALS. In contrast, aspirin did not have any effect on the reduction of the risk of ALS occurrence. | Chang, Min Cheol; Kwak, Sang Gyu; Park, Jin-Sung; Park, Donghwi | Yeungnam Univ, Coll Med, Dept Rehabil Med, Daegu, South Korea; Catholic Univ Daegu, Coll Med, Dept Med Stat, Daegu, South Korea; Kyungpook Natl Univ, Chilgok Hosp, Sch Med, Dept Neurol, Daegu, South Korea; Univ Ulsan, Coll Med, Ulsan Univ Hosp, Dept Phys Med & Rehabil, 877 Bangeojinsunghwndo Ro, Ulsan 44033, South Korea | Park, Donghwi/GYQ-6185-2022; Chang, Min Cheol/AAE-2321-2022; Kwak, Sang Gyu/AAG-4341-2021 | 23767019400; 56645812600; 44061744500; 56606561400 | bdome@hanmail.net; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 10 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2020 | 4.38 | 22.9 | 0.45 | 2025-06-25 | 18 | 16 | CYCLOOXYGENASE-2 INHIBITORS; ASPIRIN; ALS; PROSTAGLANDINS; IBUPROFEN; DISEASE; MODEL; ACID | Acetaminophen; Amyotrophic Lateral Sclerosis; Analgesics, Non-Narcotic; Animals; Anti-Inflammatory Agents, Non-Steroidal; Drug Therapy, Combination; Humans; analgesic agent; nonsteroid antiinflammatory agent; paracetamol; amyotrophic lateral sclerosis; animal; combination drug therapy; human; meta analysis; metabolism; pathology | English | 2020 | 2020-09-08 | 10.1038/s41598-020-71813-1 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | The histone lysine methyltransferase SETD8 regulates angiogenesis through HES-1 in human umbilical vein endothelial cells | Histone modifications, including histone lysine methylation, regulate gene expression in the vasculature, and targeting tumor blood vessels through histone modification decreases tumor growth. SETD8, a methyltransferase that catalyzes the mono-methylation of histone H4 lysine 20 is known to promote tumorigenesis in various cancers and its high levels of expression are related to poor prognosis. However, the detailed mechanisms by which SETD8 stimulates tumor progression and angiogenesis are still not well understood. Recent studies have demonstrated that, in vitro, BVT-948 efficiently and selectively suppresses SETD8 activity and histone methylation levels. In this study, we showed that BVT-948-mediated SETD8 inhibition in HUVECs results in an inhibition of angiogenesis. Inhibition of SETD8 not only inhibited angiogenesis but also disrupted actin stress fiber formation and induced cell cycle arrest at S phase. These effects were accompanied by increased HES-1 expression levels, decreased osteopontin levels, and a decreased differentiation of human induced pluripotent stem cells into endothelial cells. Interestingly, BVT-948 treatment reduced pathological angiogenesis in mouse OIR model. These data illustrate the mechanisms by which SETD8 regulates angiogenesis and may enable the use of a SETD8 inhibitor to treat various pathological conditions that are known to be associated with excessive angiogenesis, including and tumor growth. | Choi, Dong Kyu; Kim, Young Kyu; Park, Sang Wook; Lee, Heejin; Lee, Seul; Kim, Sang A.; Kim, Soo Jin; Lee, Junyeop; Kim, Wanil; Min, Sang-Hyun; Yu, Ji Hoon | DGMIF, New Drug Dev Ctr, 80 Chumbok Ro, Daegu, South Korea; Kyungpook Natl Univ, Sch Life Sci & Biotechnol, BK21 Plus KNU Creat BioRes Grp, Daegu, South Korea; Yeungnam Univ, Coll Med, Daegu, South Korea; Univ Ulsan, Coll Med, Asan Med Ctr, Dept Ophthalmol, Seoul, South Korea; Daegu Haany Univ, Dept Cosmet Sci & Technol, Gyoengsan Si, Gyeongsangbuk D, South Korea | ; Choi, dongKyu/LKL-2959-2024; Park, Sang-Wook/LEM-5116-2024; Lee, Junyeop/K-4940-2016 | 57215816624; 57218213799; 57218210245; 57202875112; 59850165800; 57212088958; 57212088908; 35071403300; 7405813437; 7202852238; 14526268100 | shmin03@dgmif.re.kr;yujihoon@dgmif.re.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 10 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2020 | 4.38 | 22.9 | 0.36 | 2025-06-25 | 11 | 11 | MESENCHYMAL TRANSITION; TUMOR ANGIOGENESIS; EXPRESSION; PR-SET7; GENE; H4; METHYLATION; PROGRESSION; MODULATION; MIGRATION | Actins; Biomarkers; Cell Line, Tumor; Cell Proliferation; Gene Expression Regulation; Histone-Lysine N-Methyltransferase; Human Umbilical Vein Endothelial Cells; Humans; Neovascularization, Pathologic; Neovascularization, Physiologic; Transcription Factor HES-1; actin; biological marker; HES1 protein, human; histone lysine methyltransferase; KMT5A protein, human; transcription factor HES 1; angiogenesis; cell proliferation; gene expression regulation; genetics; human; metabolism; neovascularization (pathology); tumor cell line; umbilical vein endothelial cell | English | 2020 | 2020-07-21 | 10.1038/s41598-020-69103-x | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | The prognostic role of preoperative serum albumin/globulin ratio in patients with non-metastatic renal cell carcinoma undergoing partial or radical nephrectomy | This multi-institutional study sought to clarify the association between the preoperative serum albumin/globulin ratio (AGR) and the prognosis of renal cell carcinoma (RCC) in a large cohort. This study encompassed eight institutions and 2,970 non-metastatic RCC patients who underwent a radical or partial nephrectomy from the Korean RCC (KORCC) database. A low AGR (1,143 patients; 38.5%) was defined as a preoperative AGR of less than 1.47 and a high AGR (1,827 patients; 61.5%) was defined as that 1.47 or greater. In the low AGR group, older age, female gender, the incidence of symptom presentation when diagnosed, diabetes, and hypertension was higher than in the high AGR group. Patients with low AGRs showed more progressive tumor stages with higher Fuhrman nuclear grades (all P-values<0.05). Patients in the low AGR group had a significantly lower overall survival rate (OS) and recurrence-free survival rate (RFS) in the Kaplan-Meier curves (all P-values<0.05). AGR was an independent prognostic factor for predicting the OS and RFS in the multivariate analysis (all P-values<0.05). The preoperative AGR is approachable and economical to use clinically for estimating the prognosis of RCC patients treated with surgery. | Chung, Jae-Wook; Park, Dong Jin; Chun, So Young; Choi, Seock Hwan; Lee, Jun Nyung; Kim, Bum Soo; Kim, Hyun Tae; Kim, Tae-Hwan; Yoo, Eun Sang; Byun, Seok-Soo; Hwang, Eu Chang; Kang, Seok Ho; Hong, Sung-Hoo; Chung, Jinsoo; Kwak, Cheol; Kim, Yong- June; Ha, Yun-Sok; Kwon, Tae Gyun | Kyungpook Natl Univ, Sch Med, Chilgok Hosp, Dept Urol, 807 Hoguk Ro, Daegu, South Korea; Seoul Natl Univ, Coll Med, Bundang Hosp, Dept Urol, Seongnam, South Korea; Chonnam Natl Univ, Dept Urol, Sch Med, Gwangju, Jeonnam, South Korea; Korea Univ, Dept Urol, Sch Med, Seoul, South Korea; Catholic Univ Korea, Dept Urol, Coll Med, Seoul, South Korea; Natl Canc Ctr, Dept Urol, Goyang, South Korea; Seoul Natl Univ, Dept Urol, Coll Med, Seoul, South Korea; Chungbuk Natl Univ, Dept Urol, Coll Med, Cheongju, South Korea | Kwak, Cheol/J-2731-2012; Kim, Tae-Hwan/M-3962-2017; Hwang, Eu/K-3680-2019; Kim, Soo-Yeon/ADR-9663-2022; Kim, Yong-June/E-5622-2012 | 35204798500; 59089772900; 8688166900; 9742645500; 16301364600; 57202817150; 55739531300; 57797823600; 7006609239; 7004818488; 8441681300; 7405684686; 37030299600; 16678454900; 7005639032; 26422204800; 35487226400; 15073765400 | yunsokha@gmail.com;tgkwon@knu.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 10 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2020 | 4.38 | 22.9 | 0.63 | 2025-06-25 | 17 | 17 | CORTICOSTEROID-BINDING GLOBULIN; PLASMA PROTEOME DATABASE; PRETREATMENT ALBUMIN; CURATIVE RESECTION; CANCER STATISTICS; BREAST-CANCER; INFLAMMATION; PROTEINS; SURVIVAL; PATHOPHYSIOLOGY | Carcinoma, Renal Cell; Disease-Free Survival; Female; Globulins; Humans; Kaplan-Meier Estimate; Kidney Neoplasms; Male; Middle Aged; Multivariate Analysis; Neoplasm Metastasis; Nephrectomy; Prognosis; ROC Curve; Serum Albumin; globulin; serum albumin; blood; disease free survival; female; human; Kaplan Meier method; kidney tumor; male; metabolism; metastasis; middle aged; multivariate analysis; nephrectomy; pathology; prognosis; receiver operating characteristic; renal cell carcinoma | English | 2020 | 2020-07-20 | 10.1038/s41598-020-68975-3 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Transcriptomic analysis of Dubas bug (Ommatissus lybicus Bergevin) infestation to Date Palm | Date palm (Phoenix dactylifera L.) and its fruit possess sociocultural, health and economic importance in Middle East. The date palm plantations are prone to Dubas bug (DB; Ommatissus lybicus DeBergevin; Homoptera: Tropiduchidae) attacks that severely damages the tree's growth and reduces fruit production. However, the transcriptome related datasets are not known to understand how DB activates physiological and gene regulatory mechanisms during infestation. Hence, we performed RNA-Seq of leaf infected with or without DB to understand the molecular responses of date palm seedlings. Before doing that, we noticed that DB infestation significantly increase superoxide anion and malondialdehyde production to two-folds as compared to healthy control. Stress-responsive genes such as proline transporter 2, NADP-dependent glyceraldehyde and superoxide dismutase were found significantly upregulated in infected seedlings. The infection repercussions were also revealed by significantly higher contents of endogenous phytohormonal signaling of jasmonic acid (JA) and salicylic acid (SA) compared with control. These findings persuaded to dig out intrinsic mechanisms and gene regulatory networks behind DB infestation to date palm by RNA-Seq analysis. Transcriptome analysis revealed upregulation of 6,919 genes and down-regulation of 2,695 genes in leaf during the infection process. The differentially expressed genes were mostly belongs to cellular functions (calcium and MAPK), phytohormones (auxin, gibberellins, abscisic acid, JA and SA), and secondary metabolites (especially coumarinates and gossypol). The data showed that defense responses were aggravated by gene networks involved in hypersensitive responses (PAR1, RIN4, PBS1 etc.). In conclusion, the results revealed that date palm's leaf up-regulates both cellular and phytohormonal determinants, followed by intrinsic hypersensitive responses to counter infestation process by Dubas bug. | Khan, Abdul Latif; Asaf, Sajjad; Khan, Adil; Khan, Arif; Imran, Muhammad; Al-Harrasi, Ahmed; Lee, In-Jung; Al-Rawahi, Ahmed | Univ Nizwa, Nat & Med Sci Res Ctr, Nizwa 616, Oman; Nord Univ, Fac Biosci & Aquaculture, Genom Grp, N-8049 Bodo, Norway; Kyungpook Natl Univ, Sch Appl Biosci, Daegu, South Korea | Khan, Adil/AAC-5160-2022; Khan, Abdul/H-5910-2011; Imran, Muhammad/AFL-6590-2022; khan, Arif/HMV-3165-2023; Asaf, Sajjad/ABA-3647-2021; Lee, In-Jung/GLS-0432-2022; Ul-Hamid, Anwar/B-7297-2015 | 26639372800; 56595059900; 57200917937; 57205385623; 58282433800; 6506093146; 16425830900; 7801308442 | latifepm78@yahoo.co.uk;aharrasi@unizwa.edu.om; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 10 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2020 | 4.38 | 22.9 | 0.27 | 2025-06-25 | 5 | 7 | PHENYLPROPANOID PATHWAY; EXPRESSION ANALYSIS; PLANT IMMUNITY; DEFENSE; ACID; RESPONSES; GROWTH; HERBIVORY; STRESS; GENES | Animals; Gene Expression Profiling; Gene Expression Regulation, Plant; Hemiptera; Infections; Phoeniceae; Plant Growth Regulators; Plant Leaves; Seedlings; Transcriptome; phytohormone; transcriptome; animal; gene expression profiling; gene expression regulation; genetics; Hemiptera; parasitology; pathogenicity; Phoenix (plant); plant leaf; seedling | English | 2020 | 2020-07-13 | 10.1038/s41598-020-67438-z | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Transglutaminase-2 regulates Wnt and FoxO3a signaling to determine the severity of osteoarthritis | Transglutaminase 2 (TG2), also known as tissue transglutaminase, is a calcium-dependent enzyme that has a variety of intracellular and extracellular substrates. TG2 not only increases in osteoarthritis (OA) tissue but also affects the progression of OA. However, it is still unclear how TG2 affects cartilage degradation in OA at the molecular level. Surgically induced OA lead to an increase of TG2 in the articular cartilage and growth plate, and it was dependent on TGF beta 1 in primary chondrocytes. The inhibition of TG2 enzymatic activity with intra-articular injection of ZDON, the peptide-based specific TG2 inhibitor, ameliorated the severity of surgically induced OA as well as the expression of MMP-3 and MMP-13. ZDON attenuated MMP-3 and MMP-13 expression in TGF beta- and calcium ionophor-etreated chondrocytes in a Runx2-independent manner. TG2 inhibition with ZDON suppressed canonical Wnt signaling through a reduction of beta-catenin, which was mediated by ubiquitination-dependent proteasomal degradation. In addition, TG2 activation by a calcium ionophore enhanced the phosphorylation of AMPK and FoxO3a and the nuclear translocation of FoxO3a, which was responsible for the increase in MMP-13. In conclusion, TG2 plays an important role in the pathogenesis of OA as a major catabolic mediator that affects the stability of beta-catenin and FoxO3a-mediated MMP-13 production. | Han, Min-Su; Jung, Youn-Kwan; Kim, Gun-Woo; Han, Seungwoo | Daegu Fatima Hosp, Fatima Res Inst, Lab Arthrit & Bone Biol, Daegu, South Korea; Gyeongsang Natl Univ Hosp, Biomed Res Inst, Jinju, Gyeongsangnam D, South Korea; Daegu Fatima Hosp, Dept Internal Med, Div Rheumatol, Daegu, South Korea; Kyungpook Natl Univ Hosp, Dept Internal Med, Div Rheumatol, 807 Hoguk Ro, Daegu 41404, South Korea | Han, Seungwoo/R-5326-2019; Han, Min/S-4827-2016; Kang, Sung-Yoon/AAG-7523-2020 | 56729629000; 9636963300; 55328560400; 55246807000 | kiefe73@gmail.com; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 10 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2020 | 4.38 | 22.9 | 0.45 | 2025-06-25 | 12 | 16 | ARTICULAR CHONDROCYTES LEADS; SKELETAL-MUSCLE; KAPPA-B; CARTILAGE; INDUCTION; HYPERTROPHY; ACTIVATION; PATHWAY; GENE; DIFFERENTIATION | Animals; beta Catenin; Calcium; Cartilage, Articular; Cells, Cultured; Forkhead Box Protein O3; Growth Plate; GTP-Binding Proteins; Male; Matrix Metalloproteinase 13; Matrix Metalloproteinase 3; Mice; Mice, Inbred C57BL; NF-kappa B; Osteoarthritis; Patient Acuity; Transforming Growth Factor beta; Transglutaminases; Wnt Signaling Pathway; beta catenin; calcium; collagenase 3; FoxO3 protein, mouse; guanine nucleotide binding protein; immunoglobulin enhancer binding protein; protein glutamine gamma glutamyltransferase; protein glutamine gamma glutamyltransferase 2; stromelysin; transcription factor FKHRL1; transforming growth factor beta; animal; articular cartilage; C57BL mouse; cell culture; epiphysis plate; male; metabolism; mouse; osteoarthritis; pathophysiology; patient acuity; Wnt signaling | English | 2020 | 2020-08-06 | 10.1038/s41598-020-70115-w | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Two-year outcomes of the treat-and-extend regimen using aflibercept for treating diabetic macular oedema | This study was performed to investigate the efficacy of the treat-and-extend regimen using aflibercept for treating diabetic macular oedema (DME). This prospective, multicentre, interventional, single-arm, 104-week clinical trial included 48 patients with DME visual impairment. The patients' eyes received five consecutive intravitreal injections (2 mg aflibercept) every four weeks with two-week adjustments based on central subfield macular thickness (CSMT) changes. Injections were deferred when CSMT was stable. The number of injections, best-corrected visual acuity (BCVA), CSMT, and diabetic retinopathy severity scale scores were analysed. Compared to baseline, BCVA improved by +9.1 letters at 52 weeks and was maintained with+9.4-letter gain at 104 weeks (Pm (P20/40 increased from 17.4 to 43.5% (P=0.007). The mean number of injections decreased from 8.5 in year one to 3.9 in year two. The injection interval was extended to >= 12 weeks in 56.5% of patients. The treat-and-extend regimen of aflibercept in DME showed 2-year efficacy comparable to that of fixed dosing regimens. The flexible dosing of this regimen reduced the number of injections in year two while maintaining efficacy. | Kim, Yu Cheol; Shin, Jae Pil; Pak, Kang Yeun; Kim, Hyun Woong; Sagong, Min; Lee, Sang Joon; Chung, In Young; Park, Sung Who; Lee, Ji Eun | Keimyung Univ, Dept Ophthalmol, Sch Med, Daegu, South Korea; Kyungpook Natl Univ, Dept Ophthalmol, Sch Med, Daegu, South Korea; Inje Univ, Haeundae Paik Hosp, Dept Ophthalmol, Coll Med, Busan, South Korea; Yeungnam Univ, Dept Ophthalmol, Coll Med, Daegu, South Korea; Kosin Univ, Dept Ophthalmol, Coll Med, Busan, South Korea; Gyeongsang Natl Univ, Dept Ophthalmol, Sch Med, Jinju, South Korea; Pusan Natl Univ, Sch Med, Dept Ophthalmol, 49 Busandaehak Ro, Yangsan 50612, Gyeongsangnam D, South Korea; Pusan Natl Univ Hosp, Inst Biomed, Busan, South Korea | Lee, Jae/AAA-2678-2021; Lee, Ji Eun/LIG-6337-2024; Kim, Yucheol/LRS-9072-2024 | 57209137254; 56517350400; 56098649900; 57206210671; 32267459200; 57203598185; 8561133700; 57191670530; 35215855800 | jlee@pusan.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 10 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2020 | 4.38 | 22.9 | 0.77 | 2025-06-25 | 19 | 19 | INTRAVITREAL AFLIBERCEPT; RANIBIZUMAB | Diabetic Retinopathy; Female; Humans; Macula Lutea; Macular Edema; Male; Middle Aged; Receptors, Vascular Endothelial Growth Factor; Recombinant Fusion Proteins; Treatment Outcome; Visual Acuity; aflibercept; fusion protein; vasculotropin receptor; clinical trial; diabetic retinopathy; female; human; macular edema; male; middle aged; multicenter study; pathology; pathophysiology; retina macula lutea; treatment outcome; visual acuity | English | 2020 | 2020-12-16 | 10.1038/s41598-020-78954-3 | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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