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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | ○ | Article | Comparative Proteome Research in a Zebrafish Model for Vanishing White Matter Disease | Vanishing white matter (VWM) disease is a genetic leukodystrophy leading to severe neurological disease and early death. VWM is caused by bi-allelic mutations in any of the five genes encoding the subunits of the eukaryotic translation factor 2B (EIF2B). Previous studies have attempted to investigate the molecular mechanism of VWN by constructing models for each subunit of EIF2B that causes VWM disease. The underlying molecular mechanisms of the way in which mutations in EIF2B3 result in VWM are largely unknown. Based on our recent results, we generated an eif2b3 knockout (eif2b3(-/-)) zebrafish model and performed quantitative proteomic analysis between the wild-type (WT) and eif2b3(-/-) zebrafish, and identified 25 differentially expressed proteins. Four proteins were significantly upregulated, and 21 proteins were significantly downregulated in eif2b3(-/-) zebrafish compared to WT. Lon protease and the neutral amino acid transporter SLC1A4 were significantly increased in eif2b3(-/-) zebrafish, and crystallin proteins were significantly decreased. The differential expression of proteins was confirmed by the evaluation of mRNA levels in eif2b3(-/-) zebrafish, using whole-mount in situ hybridization analysis. This study identified proteins which candidates as key regulators of the progression of VWN disease, using quantitative proteomic analysis in the first EIF2B3 animal model of VWN disease. | Kim, Doeun; Lee, Yu-Ri; Choi, Tae-Ik; Kim, Se-Hee; Kang, Hoon-Chul; Kim, Cheol-Hee; Lee, Sangkyu | Kyungpook Natl Univ, Coll Pharm, FOUR Community Based Intelligent Novel Drug Disco, Daegu 41566, South Korea; Kyungpook Natl Univ, Pharmaceut Sci Res Inst, Daegu 41566, South Korea; Chungnam Natl Univ, Dept Biol, Daejeon 34134, South Korea; Yonsei Univ, Coll Med, Severance Patientss Hosp, Div Pediat Neurol,Dept Pediat,Epilepsy Res Inst, Seoul 03722, South Korea | ; Kim, Cheol-Hee/F-6278-2013; Kim, Doeun/NJR-1829-2025; Lee, Yu-Ri/AAC-2180-2021; Kim, sung-Koo/J-3859-2019 | 57219650718; 57003921900; 57189591841; 56561828600; 34770080800; 16245115100; 57209046767 | kdkdl1230@gmail.com;jhyrsanta@naver.com;c860523@naver.com;SEHEEKIM@yuhs.ac;HIPO0207@yuhs.ac;zebrakim@cnu.ac.kr;sangkyu@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 5 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.37 | 2025-07-30 | 6 | 6 | Vanishing White Matter disease; EIF2B; comparative proteomics | Comparative proteomics; EIF2B; SLC1A4; Vanishing White Matter disease | Amino Acid Transport System ASC; Animals; Animals, Genetically Modified; Disease Models, Animal; Eukaryotic Initiation Factor-2B; Humans; Leukoencephalopathies; Proteome; Proteomics; Zebrafish; Zebrafish Proteins; 4 aminobutyrate aminotransferase; activating transcription factor 4; activating transcription factor 6; alanine; amino acid; amino acid transporter; argininosuccinate synthase; asct1 protein; aspartate carbamoyltransferase; aspartic acid; beta a2 crystallin; beta a4 crystallin; beta crystallin; crygmx protein; crystallin; endopeptidase La; eukaryotic translation factor 2b3; gamma crystallin; glutamic acid; guanine nucleotide exchange factor; initiation factor 2; messenger RNA; myelin; n methyl dextro aspartic acid receptor; nestin; oligodendrocyte transcription factor 2; opsin 1; oxoglutarate dehydrogenase; proteome; psat1 protein; slc1a4 protein; unclassified drug; X box binding protein 1; yolk protein; amino acid transporter; guanine nucleotide exchange factor; proteome; zebrafish protein; animal experiment; animal model; Article; clinical evaluation; comparative proteomics; comparative study; controlled study; differentially expressed protein; disease exacerbation; down regulation; gene expression; genetic similarity; human; mass spectrometry; molecular biology; nonhuman; protein expression; upregulation; vanishing white matter disease; white matter; whole mount in situ hybridization; zebra fish; animal; disease model; genetics; leukoencephalopathy; metabolism; pathology; proteomics; transgenic animal | English | 2021 | 2021-03 | 10.3390/ijms22052707 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Comparisons of Extracellular Vesicles from Human Epidural Fat-Derived Mesenchymal Stem Cells and Fibroblast Cells | Extracellular vesicles (EVs) are generated and secreted by cells into the circulatory system. Stem cell-derived EVs have a therapeutic effect similar to that of stem cells and are considered an alternative method for cell therapy. Accordingly, research on the characteristics of EVs is emerging. EVs were isolated from human epidural fat-derived mesenchymal stem cells (MSCs) and human fibroblast culture media by ultracentrifugation. The characterization of EVs involved the typical evaluation of cluster of differentiation (CD antigens) marker expression by fluorescence-activated cell sorting, size analysis with dynamic laser scattering, and morphology analysis with transmission electron microscopy. Lastly, the secreted levels of cytokines and chemokines in EVs were determined by a cytokine assay. The isolated EVs had a typical size of approximately 30-200 nm, and the surface proteins CD9 and CD81 were expressed on human epidural fat MSCs and human fibroblast cells. The secreted levels of cytokines and chemokines were compared between human epidural fat MSC-derived EVs and human fibroblast-derived EVs. Human epidural fat MSC-derived EVs showed anti-inflammatory effects and promoted macrophage polarization. In this study, we demonstrated for the first time that human epidural fat MSC-derived EVs exhibit inflammatory suppressive potency relative to human fibroblast-derived EVs, which may be useful for the treatment of inflammation-related diseases. | Sung, Soo-Eun; Kang, Kyung-Ku; Choi, Joo-Hee; Lee, Si-Joon; Kim, KilSoo; Lim, Ju-Hyeon; Yang, Seung Yun; Kim, Seul-Ki; Seo, Min-Soo; Lee, Gun Woo | Daegu Gyeongbuk Med Innovat Fdn DGMIF, Dept Lab Anim Ctr, Daegu 41061, South Korea; Pusan Natl Univ, Life & Ind Convergence Inst, Dept Biomat Sci, BK21 Four Program, Miryang 50463, South Korea; Kyungpook Natl Univ, Coll Vet Med, 80 Daehakro, Daegu 41566, South Korea; Osong Med Innovat Fdn, New Drug Dev Ctr, Chungbuk 28160, South Korea; Yeungnam Univ, Coll Med, Med Ctr, Dept Orthoped Surg, 170 Hyonchung Ro, Daegu 42415, South Korea; KolmarBNH CO LTD, Food Sci R&D Ctr, Efficacy Evaluat Team, 61Heolleungro 8 Gil, Seoul 06800, South Korea | Yang, Seung/B-5974-2016; Lee, Sijoon/LIC-1291-2024; Yang, Seung Yun/B-5974-2016 | 56051194000; 57215079376; 55882556800; 57219164019; 35272034300; 57218821150; 56472969700; 57208163685; 35254332100; 55599978600 | sesung@dgmif.re.kr;kangkk@dgmif.re.kr;cjh522@dgmif.re.kr;sjlee1013@dgmif.re.kr;kslac@dgmif.re.kr;sinistemcells@kbiohealth.kr;syang@pusan.ac.kr;lovesshot@kolmarbnh.co.kr;msseo@dgmif.re.kr;gwlee1871@gmail.com; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 6 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.66 | 2025-07-30 | 9 | 9 | extracellular vesicle; mesenchymal stem cell; epidural fat; fibroblast; inflammation; cytokine; chemokine | Chemokine; Cytokine; Epidural fat; Extracellular vesicle; Fibroblast; Inflammation; Mesenchymal stem cell | Animals; Cell Differentiation; Cell Polarity; Cell- and Tissue-Based Therapy; Chemokines; Cytokines; Extracellular Vesicles; Fibroblasts; Gene Expression Regulation; Humans; Inflammation; Macrophages; Mesenchymal Stem Cell Transplantation; Mesenchymal Stem Cells; 5' nucleotidase; CD14 antigen; CD34 antigen; CD63 antigen; CD81 antigen; CD9 antigen; cytokine; endoglin; fat; gelatinase B; granulocyte macrophage colony stimulating factor; growth factor; interleukin 10; interleukin 13; interleukin 4; interleukin 5; interleukin 6; macrophage inflammatory protein 1alpha; phorbol 13 acetate 12 myristate; receptor type tyrosine protein phosphatase C; Thy 1 membrane glycoprotein; tumor necrosis factor; vasculotropin; chemokine; cytokine; adipose derived stem cell; adipose tissue; Article; cell isolation; controlled study; cytokine production; enzyme linked immunosorbent assay; epidural fat; exosome; fibroblast; fluorescence activated cell sorting; human; human cell; lipopolysaccharide-induced inflammation; macrophage; mesenchymal stem cell; mesenchymal stem cell transplantation; nonhuman; skin fibroblast; transmission electron microscopy; ultracentrifugation; animal; biological therapy; cell differentiation; cell polarity; cytology; exosome; fibroblast; gene expression regulation; genetics; inflammation; mesenchymal stem cell; metabolism; transplantation | English | 2021 | 2021-03 | 10.3390/ijms22062889 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Costunolide Induces Apoptosis via the Reactive Oxygen Species and Protein Kinase B Pathway in Oral Cancer Cells | Oral cancer (OC) has been attracted research attention in recent years as result of its high morbidity and mortality. Costunolide (CTD) possesses potential anticancer and bioactive abilities that have been confirmed in several types of cancers. However, its effects on oral cancer remain unclear. This study investigated the potential anticancer ability and underlying mechanisms of CTD in OC in vivo and in vitro. Cell viability and anchorage-independent colony formation assays were performed to examine the antigrowth effects of CTD on OC cells; assessments for migration and invasion of OC cells were conducted by transwell; Cell cycle and apoptosis were investigated by flow cytometry and verified by immunoblotting. The results revealed that CTD suppressed the proliferation, migration and invasion of oral cancer cells effectively and induced cell cycle arrest and apoptosis; regarding the mechanism, CTD bound to AKT directly by binding assay and repressed AKT activities through kinase assay, which thereby downregulating the downstream of AKT. Furthermore, CTD remarkably promotes the generation of reactive oxygen species by flow cytometry assay, leading to cell apoptosis. Notably, CTD strongly suppresses cell-derived xenograft OC tumor growth in an in vivo mouse model. In conclusion, our results suggested that costunolide might prevent progression of OC and promise to be a novel AKT inhibitor. | Huang, Hai; Yi, Jun-Koo; Lim, Su-Geun; Park, Sijun; Zhang, Haibo; Kim, Eungyung; Jang, Soyoung; Lee, Mee-Hyun; Liu, Kangdong; Kim, Ki-Rim; Kim, Eun-Kyong; Lee, Youngkyun; Kim, Sung-Hyun; Ryoo, Zae-Young; Kim, Myoung Ok | Kyungpook Natl Univ, Dept Anim Sci & Biotechnol, ITRD, Sangju 37224, South Korea; Gyeongbuk Livestock Res Inst, Yeongju 36052, South Korea; Kyungpook Natl Univ, Sch Life Sci, Daegu 41566, South Korea; Dongshin Univ, Coll Korean Med, Naju 58245, South Korea; Zhengzhou Univ, Sch Basic Med Sci, Pathophysiol Dept, Zhengzhou 450008, Peoples R China; Kyungpook Natl Univ, Dept Dent Hyg, Sangju 37224, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Biochem, Daegu 41566, South Korea; Korea Polytech Coll, Dept Biomed Anal, Chungnam 34134, South Korea | ; Yi, Junkoo/JBR-8507-2023; RYOO, ZAEYOUNG/AAQ-1573-2020; Zhang, Haibo/HLP-9266-2023 | 57215021952; 56182537200; 56044587400; 54682212300; 57221648126; 57217871658; 57139360300; 58960253600; 56890019100; 35793746200; 55915892100; 36062942200; 59103241900; 16937104900; 8934745900 | huanghai1227@126.com;79lee38@korea.kr;sugeun624@hanmail.net;mooook15@naver.com;zhanghaibo615@163.com;wjddn5460@naver.com;wkdthdud21@naver.com;mhlee@dsu.ac.kr;kdliu@zzu.edu.cn;rim0804@knu.ac.kr;ekkim99@knu.ac.kr;ylee@knu.ac.kr;shkim92@kopo.ac.kr;jaewoong64@knu.ac.kr;ok4325@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 14 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 1.03 | 2025-07-30 | 13 | 15 | costunolide; ROS; AKT pathway; apoptosis; oral cancer | CYCLE ARREST; ROS; GENERATION; BIOMARKERS; RESISTANCE; GROWTH; HEAD | AKT pathway; Apoptosis; Costunolide; Oral cancer; ROS | Animals; Antineoplastic Agents, Phytogenic; Apoptosis; Cell Cycle; Cell Movement; Cell Proliferation; Gene Expression Regulation, Neoplastic; Humans; Membrane Potential, Mitochondrial; Mice; Mice, Nude; Mouth Neoplasms; Proto-Oncogene Proteins c-akt; Reactive Oxygen Species; Sesquiterpenes; Signal Transduction; Tumor Cells, Cultured; Xenograft Model Antitumor Assays; AKT1 protein, human; antineoplastic agent; costunolide; protein kinase B; reactive oxygen metabolite; sesquiterpene; animal; apoptosis; cell cycle; cell motion; cell proliferation; drug effect; drug screening; gene expression regulation; genetics; human; metabolism; mitochondrial membrane potential; mouse; mouth tumor; nude mouse; pathology; signal transduction; tumor cell culture | English | 2021 | 2021-07 | 10.3390/ijms22147509 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Review | Current Perspectives on the Physiological Activities of Fermented Soybean-Derived Cheonggukjang | Cheonggukjang (CGJ, fermented soybean paste), a traditional Korean fermented dish, has recently emerged as a functional food that improves blood circulation and intestinal regulation. Considering that excessive consumption of refined salt is associated with increased incidence of gastric cancer, high blood pressure, and stroke in Koreans, consuming CGJ may be desirable, as it can be made without salt, unlike other pastes. Soybeans in CGJ are fermented by Bacillus strains (B. subtilis or B. licheniformis), Lactobacillus spp., Leuconostoc spp., and Enterococcus faecium, which weaken the activity of putrefactive bacteria in the intestines, act as antibacterial agents against pathogens, and facilitate the excretion of harmful substances. Studies on CGJ have either focused on improving product quality or evaluating the bioactive substances contained in CGJ. The fermentation process of CGJ results in the production of enzymes and various physiologically active substances that are not found in raw soybeans, including dietary fiber, phospholipids, isoflavones (e.g., genistein and daidzein), phenolic acids, saponins, trypsin inhibitors, and phytic acids. These components prevent atherosclerosis, oxidative stress-mediated heart disease and inflammation, obesity, diabetes, senile dementia, cancer (e.g., breast and lung), and osteoporosis. They have also been shown to have thrombolytic, blood pressure-lowering, lipid-lowering, antimutagenic, immunostimulatory, anti-allergic, antibacterial, anti-atopic dermatitis, anti-androgenetic alopecia, and anti-asthmatic activities, as well as skin improvement properties. In this review, we examined the physiological activities of CGJ and confirmed its potential as a functional food. | Kim, Il-Sup; Hwang, Cher-Won; Yang, Woong-Suk; Kim, Cheorl-Ho | Kyungpook Natl Univ, Adv Bioresource Res Ctr, Daegu 41566, South Korea; Handong Global Univ, Global Leadership Sch, Pohang 37554, South Korea; Nodaji Co Ltd, Pohang 37927, South Korea; Sungkyunkwan Univ, Dept Biol Sci, Mol & Cellular Glycobiol Unit, Suwon 16419, South Korea; Sungkyunkwan Univ, Samsung Adv Inst Hlth Sci & Technol SAIHST, Seoul 06351, South Korea | ; Kim, Cheorl-Ho/T-6753-2019 | 55477678200; 36787652900; 57069270500; 7409877266 | 92kis@hanmail.net;chowon@handong.edu;yangws91@naver.com;chkimbio@skku.edu; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 22 | 11 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 1.19 | 2025-07-30 | 42 | 48 | fermented soybean paste; cheonggukjang; bioactive molecule; biological activity; human health benefit | LONG-TERM CONSUMPTION; PRODUCT CHEONGGUKJANG; FIBRINOLYTIC ENZYME; ANGIOTENSIN-II; PHYTIC ACID; IN-VITRO; IMMUNOSTIMULATORY ACTIVITY; OVERWEIGHT/OBESE SUBJECTS; ATOPIC-DERMATITIS; OXIDATIVE STRESS | Bioactive molecule; Biological activity; Cheonggukjang; Fermented soybean paste; Human health benefit | Anti-Infective Agents; Antihypertensive Agents; Antineoplastic Agents, Phytogenic; Antioxidants; Biological Products; Fermentation; Food Ingredients; Functional Food; Hypoglycemic Agents; Hypolipidemic Agents; Molecular Structure; Nutrition Assessment; Osteogenesis; Probiotics; Soybeans; anticoagulant agent; antidiabetic agent; antihypertensive agent; antiinfective agent; antiinflammatory agent; antilipemic agent; antineoplastic agent; antiobesity agent; antioxidant; daidzein; genistein; isoflavone derivative; nutraceutical; phenol derivative; phospholipid; phytic acid; probiotic agent; saponin; trypsin inhibitor; biological product; food ingredient; probiotic agent; antidiabetic activity; antihypertensive activity; antihypertensive therapy; antiinflammatory activity; antimicrobial activity; antineoplastic activity; antiobesity activity; antiosteoporotic activity; antioxidant activity; antiproliferative activity; antithrombotic activity; biological activity; blood pressure regulation; cognition; dietary fiber; enzyme activity; fermented soybean; functional food; human; immunostimulation; Korea; nonhuman; nutritional value; oxidative stress; Review; skin function; bone development; chemical structure; chemistry; drug effect; fermentation; metabolism; microbiology; nutritional assessment; soybean | English | 2021 | 2021-06 | 10.3390/ijms22115746 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Differential Angiogenic Potential of 3-Dimension Spheroid of HNSCC Cells in Mouse Xenograft | The experimental animal model is still essential in the development of new anticancer drugs. We characterized mouse tumors derived from two-dimensional (2D) monolayer cells or three-dimensional (3D) spheroids to establish an in vivo model with highly standardized conditions. Primary cancer-associated fibroblasts (CAFs) were cultured from head and neck squamous cell carcinoma (HNSCC) tumor tissues and co-injected with monolayer cancer cells or spheroids into the oral mucosa of mice. Mice tumor blood vessels were stained, followed by tissue clearing and 3D Lightsheet fluorescent imaging. We compared the effect of exosomes secreted from 2D or 3D culture conditions on the angiogenesis-related genes in HNSCC cells. Our results showed that both the cells and spheroids co-injected with primary CAFs formed tumors. Interestingly, vasculature was abundantly distributed inside the spheroid-derived but not the monolayer-derived mice tumors. In addition, cisplatin injection more significantly decreased spheroid-derived but not monolayer-derived tumor size in mice. Additionally, exosomes isolated from co-culture media of FaDu spheroid and CAF upregulated angiogenesis-related genes in HNSCC cells as compared to exosomes from FaDu cell and CAF co-culture media under in vitro conditions. The mouse tumor xenograft model derived from 3D spheroids of HNSCC cells with primary CAFs is expected to produce reliable chemotherapy drug screening results given the robust angiogenesis and lack of necrosis inside tumor tissues. | Choi, So-Young; Kang, Soo Hyun; Oh, Su Young; Lee, Kah Young; Lee, Heon-Jin; Gum, Sangil; Kwon, Tae-Geon; Kim, Jin-Wook; Lee, Sung-Tak; Hong, Yoo Jin; Kim, Dae-Geon; Hong, Su-Hyung | Kyungpook Natl Univ, Sch Dent, Dept Oral & Maxillofacial Surg, Daegu 41940, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Microbiol & Immunol, Daegu 41940, South Korea; Binaree Co Ltd, Daegu 41940, South Korea | 57202918688; 57204021325; 57204016703; 57226450171; 36462383000; 55304544700; 35205433300; 55862646000; 55931708300; 57226440163; 57226434973; 8691449100 | dentalchoi@knu.ac.kr;black_bean@knu.ac.kr;oohsuy@knu.ac.kr;christin_a@naver.com;heonlee@knu.ac.kr;gold780807@hanmail.net;kwondk@knu.ac.kr;vocaleo@knu.ac.kr;st0907@knu.ac.kr;yoojin.hong34@gmail.com;ktg4280@naver.com;hongsu@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 15 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.66 | 2025-07-30 | 14 | 14 | oral squamous cell carcinoma; head and neck squamous cell carcinoma; spheroid; cancer-associated fibroblast; tissue clearing; angiogenesis; exosome | CANCER-ASSOCIATED FIBROBLASTS; TUMOR; RESISTANCE; HYPOXIA; PROGRESSION; EXOSOMES; EMT | Angiogenesis; Cancer-associated fibroblast; Exosome; Head and neck squamous cell carcinoma; Oral squamous cell carcinoma; Spheroid; Tissue clearing | Animals; Cancer-Associated Fibroblasts; Carcinoma, Squamous Cell; Exosomes; Female; Head and Neck Neoplasms; Humans; Male; Mice; Mice, Inbred BALB C; Mouth Neoplasms; Neovascularization, Pathologic; Primary Cell Culture; Spheroids, Cellular; Tumor Cells, Cultured; Xenograft Model Antitumor Assays; animal; Bagg albino mouse; cancer associated fibroblast; drug screening; exosome; female; head and neck tumor; human; male; metabolism; mouse; mouth tumor; multicellular spheroid; neovascularization (pathology); pathology; primary cell culture; procedures; squamous cell carcinoma; tumor cell culture | English | 2021 | 2021-08 | 10.3390/ijms22158245 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Effects of 4-Hexylresorcinol on Craniofacial Growth in Rats | 4-Hexylresorcinol (4HR) has been used as a food additive, however, it has been recently demonstrated as a Class I histone deacetylase inhibitor (HDACi). Unlike other HDACi, 4HR can be taken through foods. Unfortunately, some HDACi have an influence on craniofacial growth, therefore, the purpose of this study was to evaluate the effects of 4HR on craniofacial growth. Saos-2 cells (osteoblast-like cells) were used for the evaluation of HDACi and its associated activities after 4HR administration. For the evaluation of craniofacial growth, 12.8 mg/kg of 4HR was administered weekly to 4 week old rats (male: 10, female: 10) for 12 weeks. Ten rats were used for untreated control (males: 5, females: 5). Body weight was recorded every week. Serum and head samples were collected at 12 weeks after initial administration. Craniofacial growth was evaluated by micro-computerized tomography. Serum was used for ELISA (testosterone and estrogen) and immunoprecipitation high-performance liquid chromatography (IP-HPLC). The administration of 4HR (1-100 mu M) showed significant HDACi activity (p < 0.05). Body weight was significantly different in male rats (p < 0.05), and mandibular size was significantly smaller in 4HR-treated male rats with reduced testosterone levels. However, the mandibular size was significantly higher in 4HR treated female rats with increased growth hormone levels. In conclusion, 4HR had HDACi activity in Saos-2 cells. The administration of 4HR on growing rats showed different responses in body weight and mandibular size between sexes. | Lee, In-Song; Kim, Dae-Won; Oh, Ji-Hyeon; Lee, Suk Keun; Choi, Je-Yong; Kim, Seong-Gon; Kim, Tae-Woo | Seoul Natl Univ, Sch Dent, Dept Orthodont, Seoul 3080, South Korea; Gangneung Wonju Natl Univ, Coll Dent, Dept Oral Biochem, Kangnung 28644, South Korea; Gangneung Wonju Natl Univ, Coll Dent, Dept Oral & Maxillofacial Surg, Kangnung 28644, South Korea; Inst Hydrogen Magnet React Gene Regulat, Daejeon 34140, South Korea; Kyungpook Natl Univ, Korea Mouse Phenotyping Ctr KMPC, Sch Med, Dept Biochem & Cell Biol,Cell & Matrix Res Inst, Daegu 41944, South Korea | ; Kim, Seong-Gon/AAF-7553-2020; Choi, Je-Yong/AAR-7334-2021 | 57226860150; 56194913400; 55957562000; 16417454200; 7501391068; 27171913700; 58448081700 | insonglee@gmail.com;kimdw@gwnu.ac.kr;haruna348@naver.com;sukkeunlee@hanmail.net;jechoi@knu.ac.kr;kimsg@gwnu.ac.kr;taewoo@snu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 16 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.66 | 2025-07-30 | 10 | 10 | 4-hexylresorcinol; histone deacetylase; mandible; testosterone | EPIGENETIC CONTROL; HISTONE; DIFFERENTIATION; ACETYLATION; VALPROATE; HORMONE; CELLS; MORPHOGENESIS; TESTOSTERONE; EXPRESSION | 4-hexylresorcinol; Histone deacetylase; Mandible; Testosterone | Animals; Anthelmintics; Bone and Bones; Facial Bones; Female; Hexylresorcinol; Male; Maxillofacial Development; Osteoblasts; Rats; anesthetic agent; cell protein; enfluorane; estrogen; hexylresorcinol; histone deacetylase 1; histone deacetylase 3; histone deacetylase 4; histone deacetylase 5; testosterone; unclassified drug; anthelmintic agent; hexylresorcinol; adult; animal experiment; animal tissue; Article; body weight; controlled study; craniofacial development; enzyme activity; enzyme inhibition; enzyme linked immunosorbent assay; estrogen blood level; female; high performance liquid chromatography; immunoprecipitation; male; mandible; micro-computed tomography; nonhuman; protein acetylation; rat; SaOS-2 cell line; testosterone blood level; animal; bone; cytology; drug effect; facial bone; growth, development and aging; maxillofacial development; osteoblast | English | 2021 | 2021-08 | 10.3390/ijms22168935 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Enhanced Resistance of atbzip62 against Pseudomonas syringae pv. tomato Suggests Negative Regulation of Plant Basal Defense and Systemic Acquired Resistance by AtbZIP62 Transcription Factor | The intrinsic defense mechanisms of plants toward pathogenic bacteria have been widely investigated for years and are still at the center of interest in plant biosciences research. This study investigated the role of the AtbZIP62 gene encoding a transcription factor (TF) in the basal defense and systemic acquired resistance in Arabidopsis using the reverse genetics approach. To achieve that, the atbzip62 mutant line (lacking the AtbZIP62 gene) was challenged with Pseudomonas syringae pv. tomato (Pst DC3000) inoculated by infiltration into Arabidopsis leaves at the rosette stage. The results indicated that atbzip62 plants showed an enhanced resistance phenotype toward Pst DC3000 vir over time compared to Col-0 and the susceptible disease controls, atgsnor1-3 and atsid2. In addition, the transcript accumulation of pathogenesis-related genes, AtPR1 and AtPR2, increased significantly in atbzip62 over time (0-72 h post-inoculation, hpi) compared to that of atgsnor1-3 and atsid2 (susceptible lines), with AtPR1 prevailing over AtPR2. When coupled with the recorded pathogen growth (expressed as a colony-forming unit, CFU mL(-1)), the induction of PR genes, associated with the salicylic acid (SA) defense signaling, in part explained the observed enhanced resistance of atbzip62 mutant plants in response to Pst DC3000 vir. Furthermore, when Pst DC3000 avrB was inoculated, the expression of AtPR1 was upregulated in the systemic leaves of Col-0, while that of AtPR2 remained at a basal level in Col-0. Moreover, the expression of AtAZI (a systemic acquired resistance -related) gene was significantly upregulated at all time points (0-24 h post-inoculation, hpi) in atbzip62 compared to Col-0 and atgsnor1-3 and atsid2. Under the same conditions, AtG3DPH exhibited a high transcript accumulation level 48 hpi in the atbzip62 background. Therefore, all data put together suggest that AtPR1 and AtPR2 coupled with AtAZI and AtG3DPH, with AtAZI prevailing over AtG3DPH, would contribute to the recorded enhanced resistance phenotype of the atbzip62 mutant line against Pst DC3000. Thus, the AtbZIP62 TF is proposed as a negative regulator of basal defense and systemic acquired resistance in plants under Pst DC3000 infection. | Nabi, Rizwana Begum Syed; Rolly, Nkulu Kabange; Tayade, Rupesh; Khan, Murtaza; Shahid, Muhammad; Yun, Byung-Wook | Kyungpook Natl Univ, Sch Appl Biosci, Lab Plant Funct Genom, Daegu 41566, South Korea; RDA, Natl Inst Crop Sci, Dept Southern Area Crop Sci, Miryang 50424, South Korea; Minist Agr, SENASEM, Natl Seed Serv, Natl Lab Seed Testing, 904KIN1, Kinshasa, DEM REP CONGO; Kyungpook Natl Univ, Sch Appl Biosci, Lab Plant Breeding, Daegu 41566, South Korea; Agr Res Inst Mingora, Swat 19130, Khyber Pakhtunk, Pakistan | ; Tayade, Rupesh/AAM-9652-2021; KABANGE, NKULU/AAQ-9425-2020; Shahid, Muhammad/AAE-9424-2020 | 57200232212; 57202031236; 57191753234; 57207990116; 59577718600; 8245123600 | rupesh.tayed@gmail.com;bwyun@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 21 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.96 | 2025-07-30 | 12 | 15 | disease resistance; Pseudomonas syringae pv; tomato; AtbZIP62 TF; systemic acquired resistance; Arabidopsis | DROUGHT STRESS-RESPONSE; NITRIC-OXIDE; CELL-DEATH; ARABIDOPSIS; GENES; PATHOGENS | Arabidopsis; AtbZIP62 TF; Disease resistance; Pseudomonas syringae pv. tomato; Systemic acquired resistance | Arabidopsis; Arabidopsis Proteins; Basic-Leucine Zipper Transcription Factors; Disease Resistance; Gene Expression Regulation, Plant; Gene Knockout Techniques; Genes, Plant; Phenotype; Plant Diseases; Plants, Genetically Modified; Protein Interaction Maps; Pseudomonas syringae; Reverse Genetics; salicylic acid; transcription factor; Arabidopsis protein; basic leucine zipper transcription factor; Arabidopsis; Article; bacterial growth; colony forming unit; controlled study; inoculation; nonhuman; phenotype; plant defense; plant gene; plant leaf; Pseudomonas syringae pv. tomato; reverse genetics; systemic acquired resistance; Arabidopsis; disease resistance; gene expression regulation; gene knockout; genetics; microbiology; pathogenicity; plant disease; protein analysis; Pseudomonas syringae; transgenic plant | English | 2021 | 2021-11 | 10.3390/ijms222111541 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Review | Epigenetic Targeting of Histone Deacetylases in Diagnostics and Treatment of Depression | Depression is a highly prevalent, disabling, and often chronic illness that places substantial burdens on patients, families, healthcare systems, and the economy. A substantial minority of patients are unresponsive to current therapies, so there is an urgent need to develop more broadly effective, accessible, and tolerable therapies. Pharmacological regulation of histone acetylation level has been investigated as one potential clinical strategy. Histone acetylation status is considered a potential diagnostic biomarker for depression, while inhibitors of histone deacetylases (HDACs) have garnered interest as novel therapeutics. This review describes recent advances in our knowledge of histone acetylation status in depression and the therapeutic potential of HDAC inhibitors. | Park, Hyun-Sun; Kim, Jongmin; Ahn, Seong Hoon; Ryu, Hong-Yeoul | Inje Univ, Dept Biochem, Coll Med, Busan 47392, South Korea; Sookmyung Womens Univ, Div Biol Sci, Seoul 04310, South Korea; Sookmyung Womens Univ, Res Inst Womens Hlth, Seoul 04310, South Korea; Hanyang Univ ERICA Campus, Dept Mol & Life Sci, Coll Sci & Convergence Technol, Ansan 15588, South Korea; Kyungpook Natl Univ, Sch Life Sci, Coll Natl Sci, BK21 FOUR KNU Creat BioRes Grp, Daegu 41566, South Korea | Park, Hyeonsun/GSN-5362-2022; Kim, Jong-Min/J-5435-2012 | 57189620033; 37112415400; 7401989611; 55889917800 | hspark@inje.ac.kr;jkim@sookmyung.ac.kr;hoon320@hanyang.ac.kr;rhr4757@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 10 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.78 | 2025-07-30 | 36 | 36 | histone deacetylase (HDAC); depression; biomarker; anti-depressant therapy | EARLY-LIFE STRESS; GENOME-WIDE ASSOCIATION; SYNAPTIC PLASTICITY; MAJOR DEPRESSION; SODIUM-BUTYRATE; DNA METHYLATION; MEMORY FORMATION; BDNF EXPRESSION; ANIMAL-MODEL; SIR2 GENE | Anti-depressant therapy; Biomarker; Depression; Histone deacetylase (HDAC) | Acetylation; Animals; Depression; Epigenesis, Genetic; Epigenomics; Histone Deacetylase Inhibitors; Histone Deacetylases; Humans; brain derived neurotrophic factor; butyric acid; entinostat; histone deacetylase; histone deacetylase inhibitor; vorinostat; histone deacetylase; histone deacetylase inhibitor; adult; depression; drug targeting; epigenetics; histone acetylation; human; hypothalamus hypophysis adrenal system; nerve cell plasticity; Review; acetylation; animal; depression; drug effect; epigenetics; genetic epigenesis; genetics; procedures | English | 2021 | 2021-05 | 10.3390/ijms22105398 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Farrerol Induces Cancer Cell Death via ERK Activation in SKOV3 Cells and Attenuates TNF-α-Mediated Lipolysis | Farrerol (FA) is a flavanone isolated from the Chinese herbal medicine "Man-shan-hong" (Rhododendron dauricum L.). In the present study, FA decreased the viability of SKOV3 cells in a dose- and time-dependent manner, and it induced G2/M cell cycle arrest and cell apoptosis. Cell cycle distribution analysis via flow cytometry showed that FA decreased G1 populations and increased G2/M populations in SKOV3 cells. Additionally, Western blotting confirmed an increase in the expression level of proteins involved in the cell cycle, e.g., CDK and cyclins. FA-induced apoptosis in SKOV3 cells was also investigated using a TUNEL assay, and increased expression levels of proapoptotic factors, including Caspase-3 and poly ADP ribose polymerase (PARP), through the Extracellular signal-regulated kinase (ERK)/MAPK pathway were investigated. Proinflammatory cytokines (e.g., IL-6, TNF-alpha, and IL-1) have been identified as a driver of the pathological mechanisms underlying involuntary weight loss and impaired physical function, i.e., cachexia, during cancer; in the present study, we showed that farrerol attenuates TNF-alpha-induced lipolysis and increases adipogenic differentiation in 3T3-L1 cells. Thus, farrerol could potentially be used as an anticancer agent or anticachetic drug. | Chae, Jongbeom; Kim, Jin Soo; Choi, Seok Tae; Lee, Seul Gi; Ojulari, Oyindamola Vivian; Kang, Young Jin; Kwon, Taeg Kyu; Nam, Ju-Ock | Kyungpook Natl Univ, Dept Food Sci & Biotechnol, Daegu 41566, South Korea; Natl Dev Inst Korean Med, Gyeongju Si 38540, South Korea; Yeungnam Univ, Coll Med, Dept Pharmacol, Daegu 42415, South Korea; Keimyung Univ, Sch Med, Dept Immunol, Daegu 42601, South Korea; Keimyung Univ, Ctr Forens Pharmaceut Sci, Daegu 42601, South Korea; Kyungpook Natl Univ, Inst Agr Sci & Technol, Daegu 41566, South Korea | ; KIM, Heejae/J-2173-2015 | 57204499421; 57194216639; 57222064697; 56995397800; 57205371298; 57222068266; 7202206057; 7201496105 | chejongbum@naver.com;1211pepero@naver.com;tjrxopk@naver.com;lsg100479@naver.com;hoyeendahmolar@gmail.com;yjkang@med.yu.ac.kr;kwontk@dsmc.or.kr;namjo@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 17 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.96 | 2025-07-30 | 16 | 14 | antitumor effect; adipogenesis; cachexia; cell cycle arrest; farrerol; ovarian cancer | NECROSIS-FACTOR-ALPHA; INSULIN-RESISTANCE; OVARIAN-CANCER; ADIPOCYTE DIFFERENTIATION; APOPTOSIS; PROLIFERATION; DEGRADATION; INHIBITION; MECHANISMS; EXPRESSION | Adipogenesis; Antitumor effect; Cachexia; Cell cycle arrest; Farrerol; Ovarian cancer | Apoptosis; Cell Cycle; Cell Proliferation; Chromones; Extracellular Signal-Regulated MAP Kinases; Female; Gene Expression Regulation, Neoplastic; Humans; Lipolysis; Ovarian Neoplasms; Tumor Cells, Cultured; Tumor Necrosis Factor-alpha; antineoplastic agent; beta actin; caspase 3; cyclin A; cyclin B; cyclin D; cyclin dependent kinase 1; cyclin dependent kinase 2; cyclin dependent kinase 4; cyclin E; farrerol; flavanone derivative; interleukin 1; interleukin 6; mitogen activated protein kinase; mitogen activated protein kinase p38; nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase; peroxisome proliferator activated receptor gamma; regulator protein; triacylglycerol; tumor necrosis factor; unclassified drug; uncoupling protein 1; chromone derivative; farrerol; mitogen activated protein kinase; tumor necrosis factor; 3T3-L1 cell line; antineoplastic activity; apoptosis; Article; body weight loss; cachexia; cell differentiation; cell proliferation; cell viability; controlled study; DNA fragmentation; EA.hy 926 cell line; enzyme activation; female; flow cytometry; G2 phase cell cycle checkpoint; human; human cell; lipid storage; lipolysis; MAPK signaling; MTT assay; protein expression; protein expression level; real time reverse transcription polymerase chain reaction; SK-OV-3 cell line; TUNEL assay; Western blotting; apoptosis; cell cycle; drug effect; gene expression regulation; lipolysis; metabolism; ovary tumor; pathology; tumor cell culture | English | 2021 | 2021-09 | 10.3390/ijms22179400 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Flavonone 3-hydroxylase Relieves Bacterial Leaf Blight Stress in Rice via Overaccumulation of Antioxidant Flavonoids and Induction of Defense Genes and Hormones | Efficient accumulation of flavonoids is important for increased tolerance to biotic stress. Although several plant defense mechanisms are known, the roles of many pathways, proteins, and secondary metabolites in stress tolerance are unknown. We generated a flavanone 3-hydroxylase (F3H) overexpressor rice line and inoculated Xanthomonas Oryzae pv. oryzae and compared the control and wildtype inoculated plants. In addition to promoting plant growth and developmental maintenance, the overexpression of F3H increased the accumulation of flavonoids and increased tolerance to bacterial leaf blight (BLB) stress. Moreover, leaf lesion length was higher in the infected wildtype plants compared with infected transgenics. Kaempferol and quercetin, which scavenge reactive oxygen species, overaccumulated in transgenic lines compared with wildtypes in response to pathogenic infection, detected by scanning electron microscopy and spectrophotometry. The induction of F3H altered the antioxidant system and reduced the levels of glutathione peroxidase activity and malondialdehyde (MDA) contents in the transgenic lines compared with the wildtypes. Downstream gene regulation analysis showed that the expression of F3H increased the regulation of flavonol synthase (FLS), dihydroflavonol 4-reductase (DFR), and slender rice mutant (SLR1) during BLB stress. The analysis of SA and JA signaling revealed an antagonistic interaction between both hormones and that F3H induction significantly promoted SA and inhibited JA accumulation in the transgenic lines. SA-dependent nonexpressor pathogenesis-related (NPR1) and Xa1 showed significant upregulation in the infected transgenic lines compared with the infected control and wildtype lines. Thus, the overexpression of F3H was essential for increasing BLB stress tolerance. | Jan, Rahmatullah; Aaqil Khan, Muhammad; Asaf, Sajjad; Lubna; Park, Jae-Ryoung; Lee, In-Jung; Kim, Kyung-Min | Kyungpook Natl Univ, Coll Agr & Life Sci, Dept Appl Biosci, Div Plant Biosci, 80 Dahak Ro, Daegu 41566, South Korea; Kyungpook Natl Univ, Costal Agr Res Inst, 80 Dahak Ro, Daegu 41566, South Korea; Univ Nizwa, Nat & Med Sci Res Ctr, Nizwa 616, Oman; Abdul Wali Khan Univ, Dept Bot, Garden Campus, Marda 23200, Pakistan | ; Asaf, Sajjad/ABA-3647-2021; Khan, Muhammad/ABB-9797-2021; Kim, Kyung-Min Kim/C-7007-2014; Lee, In-Jung/GLS-0432-2022; Jan, Rahmatullah/AIC-3439-2022 | 57201981969; 57188585606; 56595059900; 57200621537; 57211205505; 16425830900; 34868260300 | rehmatbot@yahoo.com;aqil_bacha@yahoo.com;sajadasif2000@gmail.com;lubnabilal68@gmail.com;icd92@naver.com;ijlee@knu.ac.kr;kkm@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 11 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 2.36 | 2025-07-30 | 34 | 37 | bacterial leaf blight; kaempferol; antioxidant; malondialdehyde; antagonistic | SALICYLIC-ACID; SECONDARY METABOLITES; ARABIDOPSIS-THALIANA; ANTHOCYANIN BIOSYNTHESIS; DROUGHT TOLERANCE; RESISTANCE; PLANTS; JASMONATE; SUPPRESSION; REGULATORS | Antagonistic; Antioxidant; Bacterial leaf blight; Kaempferol; Malondialdehyde | Antioxidants; Disease Resistance; Flavonoids; Gene Expression Regulation, Plant; Hormones; Mixed Function Oxygenases; Oryza; Plant Diseases; Plant Leaves; Plant Proteins; Plants, Genetically Modified; Stress, Physiological; Xanthomonas; amino acid; anthocyanin; antioxidant; arginine; ascorbic acid; aspartic acid; carotenoid; catalase; chlorophyll; dihydroflavonol 4 reductase; flavanone; flavonoid; flavonol; flavonol synthase; flavonone 3 hydroxylase; glucoside; glutathione; glutathione peroxidase; isoquercetin; kaempferol; malonaldehyde; naringenin; oxidoreductase; oxygenase; proline; quercetin; reactive oxygen metabolite; reduced nicotinamide adenine dinucleotide phosphate; superoxide dismutase; synthetase; unclassified drug; antioxidant; flavanone 3-dioxygenase; flavonoid; hormone; mixed function oxidase; plant protein; antioxidant activity; Article; bacterial leaf blight; biosynthesis; biotic stress; chlorophyll content; controlled study; enzyme activity; gas chromatography; gene control; gene overexpression; immunoblotting; mass spectrometry; nonhuman; phenotype; plant defense; plant growth; plant immunity; plant leaf; protein expression; rice; scanning electron microscopy; signal transduction; spectrophotometry; transgene; transgenics; upregulation; Xanthomonas oryzae pv. oryzae; disease resistance; gene expression regulation; genetics; immunology; metabolism; microbiology; Oryza; physiological stress; physiology; plant disease; transgenic plant; Xanthomonas | English | 2021 | 2021-06 | 10.3390/ijms22116152 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Review | Genetic Therapy for Intervertebral Disc Degeneration | Intervertebral disc (IVD) degeneration can cause chronic lower back pain (LBP), leading to disability. Despite significant advances in the treatment of discogenic LBP, the limitations of current treatments have sparked interest in biological approaches, including growth factor and stem cell injection, as new treatment options for patients with chronic LBP due to IVD degeneration (IVDD). Gene therapy represents exciting new possibilities for IVDD treatment, but treatment is still in its infancy. Literature searches were conducted using PubMed and Google Scholar to provide an overview of the principles and current state of gene therapy for IVDD. Gene transfer to degenerated disc cells in vitro and in animal models is reviewed. In addition, this review describes the use of gene silencing by RNA interference (RNAi) and gene editing by the clustered regularly interspaced short palindromic repeats (CRISPR) system, as well as the mammalian target of rapamycin (mTOR) signaling in vitro and in animal models. Significant technological advances in recent years have opened the door to a new generation of intradiscal gene therapy for the treatment of chronic discogenic LBP. | Roh, Eun Ji; Darai, Anjani; Kyung, Jae Won; Choi, Hyemin; Kwon, Su Yeon; Bhujel, Basanta; Kim, Kyoung Tae; Han, Inbo | CHA Univ, Dept Neurosurg, CHA Bundang Med Ctr, Sch Med, Seongnam Si 13496, South Korea; CHA Univ, CHA Bundang Med Ctr, Dept Biomed Sci, Sch Med, Seongnam Si 13496, South Korea; Kyungpook Natl Univ, Sch Med, Dept Neurosurg, Daegu 41944, South Korea; Kyungpook Natl Univ Hosp, Dept Neurosurg, Daegu 41944, South Korea | 57219129044; 57221804434; 56895826800; 57193951350; 57219127846; 57221807481; 57201369790; 9338449900 | morolro@naver.com;anjanianji09@gmail.com;kyungjaewon88@gmail.com;littlechoi88@gmail.com;syunkwon@naver.com;basantabhuje186@gmail.com;nskimkt7@gmail.com;hanib@cha.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 4 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 2.08 | 2025-07-30 | 95 | 95 | genetic therapy; intervertebral disc degeneration; RNAi; mTOR signaling; CRISPR-Cas9; vector | NUCLEUS PULPOSUS CELLS; MOLECULAR-MECHANISMS; GROWTH-FACTORS; STEM-CELLS; TGF-BETA; ULTRASOUND; INTERFERENCE; APOPTOSIS; DELIVERY; CRISPR | CRISPR-Cas9; Genetic therapy; Intervertebral disc degeneration; MTOR signaling; RNAi; Vector | Animals; Gene Editing; Genetic Therapy; Genetic Vectors; Humans; Intervertebral Disc Degeneration; CRISPR associated endonuclease Cas9; growth factor; mammalian target of rapamycin; Adeno associated virus; Adenoviridae; Baculoviridae; cell therapy; CRISPR-CAS9 system; discogenic pain; gene editing; gene silencing; gene therapy; gene transfer; human; in vitro study; in vivo study; intervertebral disk degeneration; Lentivirus; low back pain; micelle; mTOR signaling; nonhuman; nonviral gene therapy; pathophysiology; posttranscriptional gene silencing; Review; RNA interference; tissue engineering; ultrasound targeted microbubble destruction; viral gene therapy; administration and dosage; animal; gene editing; gene vector; genetics; intervertebral disk degeneration | English | 2021 | 2021-02 | 10.3390/ijms22041579 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Global Lysine Acetylome Analysis of LPS-Stimulated HepG2 Cells Identified Hyperacetylation of PKM2 as a Metabolic Regulator in Sepsis | Sepsis-induced liver dysfunction (SILD) is a common event and is strongly associated with mortality. Establishing a causative link between protein post-translational modification and diseases is challenging. We studied the relationship among lysine acetylation (Kac), sirtuin (SIRTs), and the factors involved in SILD, which was induced in LPS-stimulated HepG2 cells. Protein hyperacetylation was observed according to SIRTs reduction after LPS treatment for 24 h. We identified 1449 Kac sites based on comparative acetylome analysis and quantified 1086 Kac sites on 410 proteins for acetylation. Interestingly, the upregulated Kac proteins are enriched in glycolysis/gluconeogenesis pathways in the Kyoto Encyclopedia of Genes and Genomes (KEGG) category. Among the proteins in the glycolysis pathway, hyperacetylation, a key regulator of lactate level in sepsis, was observed at three pyruvate kinase M2 (PKM2) sites. Hyperacetylation of PKM2 induced an increase in its activity, consequently increasing the lactate concentration. In conclusion, this study is the first to conduct global profiling of Kac, suggesting that the Kac mechanism of PKM2 in glycolysis is associated with sepsis. Moreover, it helps to further understand the systematic information regarding hyperacetylation during the sepsis process. | Na, Ann-Yae; Paudel, Sanjita; Choi, Soyoung; Lee, Jun Hyung; Kim, Min-Sik; Bae, Jong-Sup; Lee, Sangkyu | Kyungpook Natl Univ, Coll Pharm, BK21 FOUR Community Based Intelligent Novel Drug, Daegu 41566, South Korea; Kyungpook Natl Univ, Res Inst Pharmaceut Sci, Daegu 41566, South Korea; Daegu Gyeongbuk Inst Sci & Technol, Dept New Biol, Daegu 42988, South Korea | Kim, Min-Sik/KFS-9148-2024; Bae, Jong-Sup/AAU-9724-2020; Kim, Min-Sik/M-3488-2016 | 57201530058; 57203320448; 57202918688; 57225022774; 57192905667; 16021543200; 57209046767 | cpblady@daum.net;sanjitapd199@gmail.com;sylvdh@naver.com;jhlee8810@dgist.ac.kr;mkim@dgist.ac.kr;baejs@knu.ac.kr;sangkyu@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 16 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.52 | 2025-07-30 | 7 | 7 | sepsis-induced liver dysfunction; hyperacetylation; SIRT; lysine acetylation; pyruvate kinase M2 | SYSTEMIC INFLAMMATION; GLYCOLYSIS; LIVER; SIRT5; SHOCK | Hyperacetylation; Lysine acetylation; Pyruvate kinase M2; Sepsis-induced liver dysfunction; SIRT | Acetylation; Carrier Proteins; Hep G2 Cells; Humans; Lipopolysaccharides; Liver; Lysine; Membrane Proteins; Sepsis; Thyroid Hormones; beta actin; complementary DNA; interleukin 6; lactic acid; lipopolysaccharide; lysine; pyruvate kinase; pyruvate kinase m2; sirtuin; sirtuin 1; sirtuin 2; sirtuin 3; sirtuin 4; sirtuin 5; sirtuin 6; sirtuin 7; transcription factor RelA; unclassified drug; carrier protein; lipopolysaccharide; lysine; membrane protein; thyroid hormone; thyroid hormone-binding proteins; acetylation; Article; bioinformatics; cell stimulation; controlled study; cytokine release; gluconeogenesis; glycolysis; Hep-G2 cell line; human; human cell; immunoblotting; in vitro study; KEGG; limit of quantitation; lipopolysaccharide-induced sepsis; liquid chromatography-mass spectrometry; lysine acetylation; nano liquid chromatography-mass spectrometry; pathogenesis; protein analysis; protein expression level; protein fingerprinting; protein interaction; quantitative analysis; real time polymerase chain reaction; SILAC labeling; ultra performance liquid chromatography; upregulation; Western blotting; acetylation; drug effect; enzymology; Hep-G2 cell line; liver; metabolism; sepsis | English | 2021 | 2021-08 | 10.3390/ijms22168529 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | HNRNP A1 Promotes Lung Cancer Cell Proliferation by Modulating VRK1 Translation | THeterogeneous nuclear ribonucleoprotein (HNRNP) A1 is the most abundant and ubiquitously expressed member of the HNRNP protein family. In recent years, it has become more evident that HNRNP A1 contributes to the development of neurodegenerative diseases. However, little is known about the underlying role of HNRNP A1 in cancer development. Here, we report that HNRNP A1 expression is significantly increased in lung cancer tissues and is negatively correlated with the overall survival of patients with lung cancer. Additionally, HNRNP A1 positively regulates vaccinia-related kinase 1 (VRK1) translation via binding directly to the 3' untranslated region (UTR) of VRK1 mRNA, thus increasing cyclin D1 (CCND1) expression by VRK1-mediated phosphorylation of the cAMP response element-binding protein (CREB). Furthermore, HNRNP A1 binding to the cis-acting region of the 3'UTR of VRK1 mRNA contributes to increased lung cancer cell proliferation. Thus, our study unveils a novel role of HNRNP A1 in lung carcinogenesis via post-transcriptional regulation of VRK1 expression and suggests its potential as a therapeutic target for patients with lung cancer. | Ryu, Hye Guk; Jung, Youngseob; Lee, Namgyu; Seo, Ji-Young; Kim, Sung Wook; Lee, Kyung-Ha; Kim, Do-Yeon; Kim, Kyong-Tai | Pohang Univ Sci & Technol POSTECH, Dept Life Sci, Pohang 37673, South Korea; Pohang Univ Sci & Technol POSTECH, Div Integrat Biosci & Biotechnol, Pohang 37673, South Korea; Univ Massachusetts, Dept Mol Cell & Canc Biol, Sch Med, Worcester, MA 01065 USA; Daegu Haany Univ, Div Cosmet Sci & Technol, Gyongsan 38610, South Korea; Kyungpook Natl Univ, Dept Pharmacol, Sch Dent, Daegu 41940, South Korea | Kim, Do-Yeon/AET-3021-2022; Lee, Kyung-Ha/GRY-2640-2022; Park, Mi-Kyung/J-9643-2017 | 56611282900; 56123237500; 55330470700; 57188750232; 57205473549; 54967786000; 57203012542; 7409315595 | hyegukryu@gmail.com;ysjung@postech.ac.kr;Namgyu.Lee@umassmed.edu;jyseo@postech.ac.kr;kimsw@postech.ac.kr;kyungha.lee@dhu.ac.kr;dykim82@knu.ac.kr;ktk@postech.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 22 | 11 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 1.55 | 2025-07-30 | 26 | 26 | HNRNP A1; VRK1; lung cancer; post-transcriptional regulation; 3 ' UTR | MESSENGER-RNA; CYCLE REGULATORS; BINDING; PHOSPHORYLATES; DEGRADATION; INVASION; ROLES; GENE | 3′UTR; HNRNP A1; Lung cancer; Post-transcriptional regulation; VRK1 | 3' Untranslated Regions; Base Sequence; Cell Cycle; Cell Line; CRISPR-Cas Systems; Cyclin D1; Eukaryotic Initiation Factor-3; Gene Expression Regulation, Neoplastic; Genes, Reporter; Heterogeneous Nuclear Ribonucleoprotein A1; Humans; Intracellular Signaling Peptides and Proteins; Lung Neoplasms; Neoplasm Proteins; Protein Binding; Protein Biosynthesis; Protein Domains; Protein Interaction Mapping; Protein-Serine-Threonine Kinases; Recombinant Proteins; RNA Interference; RNA, Messenger; Sequence Deletion; Up-Regulation; cyclic AMP responsive element binding protein; cyclin D1; heterogeneous nuclear ribonucleoprotein A1; messenger RNA; protein serine threonine kinase; unclassified drug; vaccinia related kinase 1; CCND1 protein, human; cyclin D1; EIF3B protein, human; heterogeneous nuclear ribonucleoprotein A1; hnRNPA1 protein, human; initiation factor 3; messenger RNA; protein binding; protein serine threonine kinase; recombinant protein; signal peptide; tumor protein; VRK1 protein, human; 3' untranslated region; Article; cancer cell; cancer tissue; cell proliferation; controlled study; embryo; gene expression regulation; human; human cell; human tissue; lung cancer; overall survival; protein expression; protein phosphorylation; protein RNA binding; upregulation; biosynthesis; cell cycle; cell line; chemistry; CRISPR Cas system; gene deletion; gene expression regulation; genetics; lung tumor; metabolism; nucleotide sequence; pathology; physiology; protein analysis; protein domain; protein synthesis; reporter gene; RNA interference | English | 2021 | 2021-06 | 10.3390/ijms22115506 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | IFNγ Is a Key Link between Obesity and Th1-Mediated AutoImmune Diseases | Obesity, a characteristic of metabolic syndrome, is also associated with chronic inflammation and the development of autoimmune diseases. However, the relationship between obesity and autoimmune diseases remains to be investigated in depth. Here, we compared hepatic gene expression profiles among high-fat diet (HFD) mice using the primary biliary cholangitis (PBC) mouse model based on the chronic expression of interferon gamma (IFN gamma) (ARE-Del(-/-) mice). The top differentially expressed genes affected by upstream transcriptional regulators IFN gamma, LPS, and TNF alpha displayed an overlap in HFD and ARE-Del(-/-) mice, indicating that obesity-induced liver inflammation may be dependent on signaling via IFN gamma. The top pathways altered in HFD mice were mostly involved in the innate immune responses, which overlapped with ARE-Del(-/-) mice. In contrast, T cell-mediated signaling pathways were exclusively altered in ARE-Del(-/-) mice. We further evaluated the therapeutic effect of luteolin, known as anti-inflammatory flavonoid, in HFD and ARE-Del(-/-) mice. Luteolin strongly suppressed the MHC I and II antigen presentation pathways, which were highly activated in both HFD and ARE-Del(-/-) mice. Conversely, luteolin increased metabolic processes of fatty acid oxidation and oxidative phosphorylation in the liver, which were suppressed in ARE-Del(-/-) mice. Luteolin also strongly induced PPAR signaling, which was downregulated in HFD and ARE-Del(-/-) mice. Using human GWAS data, we characterized the genetic interaction between significant obesity-related genes and IFN gamma signaling and demonstrated that IFN gamma is crucial for obesity-mediated inflammatory responses. Collectively, this study improves our mechanistic understanding of the relationship between obesity and autoimmune diseases. Furthermore, it provides new methodological insights into how immune network-based analyses effectively integrate RNA-seq and microarray data. | Bae, Heekyong R.; Choi, Myung-Sook; Kim, Suntae; Young, Howard A.; Gershwin, M. Eric; Jeon, Seon-Min; Kwon, Eun-Young | Omixplus LLC, Gaithersburg, MD 20885 USA; NCI, Lab Canc Immunometab, Canc & Inflammat Program, Ctr Canc Res, Frederick, MD 21701 USA; Kyungpook Natl Univ, Ctr Food & Nutr Genom Res, Dept Food Sci & Nutr, Daegu 41566, South Korea; Univ Calif Davis, Div Rheumatol Allergy & Clin Immunol, Davis, CA 95616 USA; APtechnologies Corp, R&D Ctr, Gyeonggi Do 18469, Hwaseong Si, South Korea | Young, Howard/A-6350-2008 | 57191253762; 7402093877; 57221230845; 57202981972; 55663621200; 7203005715; 15765422500 | bae@omixplus.com;mschoi@knu.ac.kr;skim@omixplus.com;younghow@mail.nih.gov;megershwin@ucdavis.edu;smjeon111@gmail.com;eykwon@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 1 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.88 | 2025-07-30 | 12 | 14 | IFN gamma; obesity; autoimmune diseases; Th1; luteolin; high-fat diet; RNA-seq; microarray; bioinformatics; animal study | INTERFERON-GAMMA; PPAR-GAMMA; LEPTIN RESISTANCE; ADIPOSE-TISSUE; EXPRESSION; INFLAMMATION; LUTEOLIN; ACTIVATION; PHOSPHORYLATION; MACROPHAGES | Animal study; Autoimmune diseases; Bioinformatics; High-fat diet; IFNγ; Luteolin; Microarray; Obesity; RNA-seq; Th1 | Animals; Antigen Presentation; Diet, High-Fat; Energy Metabolism; Interferon-gamma; Leptin; Liver; Liver Cirrhosis, Biliary; Luteolin; Mice; Obesity; Signal Transduction; Th1 Cells; gamma interferon; leptin; lipopolysaccharide; luteolin; major histocompatibility antigen class 1; major histocompatibility antigen class 2; peroxisome proliferator activated receptor; tumor necrosis factor; gamma interferon; leptin; luteolin; animal experiment; animal model; animal tissue; antigen presentation; Article; autoimmune disease; bioinformatics; controlled study; data analysis software; DNA microarray; energy metabolism; fatty acid oxidation; gene expression profiling; gene interaction; genome-wide association study; innate immunity; lipid diet; male; mouse; mouse model; nonhuman; obesity; oxidative phosphorylation; primary biliary cirrhosis; protein expression; RNA sequencing; signal transduction; T lymphocyte; Th1 cell; therapy effect; transcription regulation; adverse event; animal; biliary cirrhosis; comparative study; complication; drug effect; genetics; immunology; liver; metabolism; obesity; Th1 cell | English | 2021 | 2021-01 | 10.3390/ijms22010208 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Review | Impacts of Drug Interactions on Pharmacokinetics and the Brain Transporters: A Recent Review of Natural Compound-Drug Interactions in Brain Disorders | Natural compounds such as herbal medicines and/or phyto-compounds from foods, have frequently been used to exert synergistic therapeutic effects with anti-brain disorder drugs, supplement the effects of nutrients, and boost the immune system. However, co-administration of natural compounds with the drugs can cause synergistic toxicity or impeditive drug interactions due to changes in pharmacokinetic properties (e.g., absorption, metabolism, and excretion) and various drug transporters, particularly brain transporters. In this review, natural compound-drug interactions (NDIs), which can occur during the treatment of brain disorders, are emphasized from the perspective of pharmacokinetics and cellular transport. In addition, the challenges emanating from NDIs and recent approaches are discussed. | Khadka, Bikram; Lee, Jae-Young; Park, Eui Kyun; Kim, Ki-Taek; Bae, Jong-Sup | Mokpo Natl Univ, Dept Biomed Hlth & Life Convergence Sci, BK21 Four, Muan Gun 58554, Jeonnam, South Korea; Chungnam Natl Univ, Coll Pharm, Daejeon 34134, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Pathol & Regenerat Med, Daegu 41940, South Korea; Mokpo Natl Univ, Coll Pharm, Muan Gun 58554, Jeonnam, South Korea; Mokpo Natl Univ, Nat Med Res Inst, Muan Gun 58554, Jeonnam, South Korea; Kyungpook Natl Univ, Res Inst Pharmaceut Sci, Coll Pharm, Daegu 41566, South Korea | ; Bae, Jong-Sup/AAU-9724-2020; Lee, Jae-Yeong/AEP-9607-2022 | 57219279407; 56195895300; 37071072400; 24488281700; 16021543200 | khadkabikram180@gmail.com;jaeyoung@cnu.ac.kr;epark@knu.ac.kr;ktkim0628@mokpo.ac.kr;baejs@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 22 | 4 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2021 | 6.208 | 23.1 | 0.14 | 2025-07-30 | 7 | 8 | natural compound– drug interactions (NDIs); pharmacokinetics; drug transporters; blood– brain barrier (BBB) and blood– cerebrospinal fluid barrier (BCSFB); brain disorders | Blood–brain barrier (BBB) and blood–cerebrospinal fluid barrier (BCSFB); Brain disorders; Drug transporters; Natural compound–drug interactions (NDIs); Pharmacokinetics | Animals; Biological Transport; Blood-Brain Barrier; Brain Diseases; Drug Interactions; Humans; Membrane Transport Proteins; Phytochemicals; Plants, Medicinal; ABC transporter; allicin; alliin; alprazolam; amitriptyline; aripiprazole; baicalin; berberine; betulic acid; bilobalide; black cumin extract; borneol; caffeine; carbamazepine; carrier protein; Catha edulis extract; clomipramine; Curcuma longa extract; curcumin; cysteine; cytochrome P450; diazepam; diosmin; docetaxel; donepezil; doxorubicin; drug metabolizing enzyme; fexofenadine; garlic extract; Ginkgo biloba extract; ginkgolide; ginkgolide B; ginsenoside; glucose; hyperforin; hypericin; Hypericum perforatum extract; lactone; lamotrigine; Lepidium sativum extract; midazolam; natural product; nimodipine; Paeonia emodi extract; Paeonia extract; pentetrazole; pentosalen; Pinus massoniana extract; piperine; plant extract; plant glycoside; Polygonum cuspidatum extract; procyanidin; protopanaxadiol; pseudohypericin; puerarin; quercetin; quinidine; resveratrol; rhamnose; rhodamine 123; ritonavir; silymarin; sinapic acid; solute carrier protein; temozolomide; terpene; topiramate; unclassified drug; valproic acid; vilazodone; vincristine; carrier protein; phytochemical; area under the curve; bile flow; blood brain barrier; blood cerebrospinal fluid barrier; brain disease; cell transport; cerebrospinal fluid; drug absorption; drug antagonism; drug bioavailability; drug clearance; drug distribution; drug excretion; drug half life; drug metabolism; drug potentiation; food drug interaction; gastrointestinal absorption; grapefruit juice; herb drug interaction; human; liver metabolism; maximum concentration; nonhuman; Review; urinary excretion; volume of distribution; animal; brain disease; drug interaction; medicinal plant; metabolism; pathology; transport at the cellular level | English | 2021 | 2021-02 | 10.3390/ijms22041809 | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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