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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | ○ | Article | Quality Evaluation and Shipping Export Potential of 'Eliteggul' Korean Melon (Cucumis melo var. makuwa) to Singapore Using MAP Technology | Korean melons (Cucumis melo var. makuwa), which have a short shelf life and are prone to browning, face challenges when exported over long-distance via maritime shipping. However, the high firmness and low suture browning rate of the 'Eliteggul' cultivar suggest its potential suitability for long-distance export. This study aimed to evaluate the export suitability of 'Eliteggul' by comparing it to the widely grown cultivar 'Alchankkul'. Consumer preferences were assessed based on fragrance, sweetness, overall preference, appearance, and firmness. Both cultivars were grown and harvested in a commercial greenhouse in Seongju-gun, South Korea. After being treated with modified atmosphere film, the fruits were stored at 6 degrees C for 1 day, transported to Singapore in a shipping container at 7.4 degrees C for 13 days, and distributed at 22 degrees C for 3 days. After 14 days of storage, analysis in Singapore showed that 'Eliteggul' maintained a significantly higher firmness at 12.9 +/- 0.3 kgcm2 compared to 'Alchankkul', which showed firmness at 8.1 +/- 0.4 kgcm2. Additionally, 'Eliteggul' exhibited less browning on the white sutures at 1.3 +/- 0.1, indicating better preservation of its quality than 'Alchankkul', at 2.0 +/- 0.3. Even at 14 + 3 days, 'Eliteggul' maintained a firmness of 12.8 +/- 0.2 kgcm2, which was significantly higher than that of 'Alchankkul', which showed a firmness of 7.7 +/- 0.5 kgcm2. Consumer preference surveys (n = 78) conducted in the local market revealed that customers favored the fragrance and sweetness of 'Alchankkul'. Correspondingly, an analysis of volatile organic compounds (VOCs) from melon juice using a Twister stir bar method showed that 'Alchankkul' had significantly higher levels of ethyl(methylthio)acetate, ethyl propanoate, and benzyl alcohol, known to contribute to a sweet flavor. Furthermore, a thermal desorption tube VOCs test indicated that hexyl acetate and 2-methylbutyl 2-methylbutyrate were also significantly higher in 'Alchankkul'. Despite this result, there was no significant difference in the overall preference scores between the two cultivars. This study indicates that 'Eliteggul', with a superior firmness, reduced browning, and high consumer acceptance, is ideal for long-distance export, offering foundational data to bridge the research gap in export-oriented melons and support the broader distribution of Korean cultivars. | Jang, Yu-Jin; Ko, Da-Yeong; Ryu, Jung-A; Kang, Min-Gu; Han, Jeung-Sul; Ku, Kang-Mo | Korea Univ, Coll Life Sci & Biotechnol, Dept Plant Biotechnol, Seoul 02841, South Korea; Gyeongsangbuk Do Agr Res & Extens Serv, Daegu 41404, South Korea; Kyungpook Natl Univ, Coll Agr & Life Sci, Dept Hort Sci, Daegu 41566, South Korea | ; Ku, Kang-Mo/JPL-8941-2023; Jang, Yu Jin/AID-7273-2022 | 59491515400; 57455993200; 57216744726; 59490234800; 8277688900; 58729600300 | yjj2465@korea.ac.kr;kodayeong@korea.ac.kr;jaryu@korea.kr;ilovegeoje@korea.kr;peterpan@knu.ac.kr;ku_km@korea.ac.kr; | HORTICULTURAE | HORTICULTURAE | 2311-7524 | 10 | 12 | SCIE | HORTICULTURE | 2024 | 3 | 14.4 | 0 | 2025-05-07 | 0 | 0 | exporting; Korean melon; modified atmosphere film; quality | VOLATILE COMPOUNDS; BIOSYNTHESIS | exporting; Korean melon; modified atmosphere film; quality | English | 2024 | 2024-12 | 10.3390/horticulturae10121354 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | Meeting Abstract | The lifetime occurrence rate, socio-demographic associations, and co-occurrence of diagnoses within the bipolar spectrum disorder among the overall populace of South Korea | Chang, Sung Man; Choi, Tae Young; Lee, Seung Jae | Kyungpook Natl Univ, Daegu, South Korea; Catholic Univ Daegu, Sch Med, Daegu, South Korea | BIPOLAR DISORDERS | BIPOLAR DISORD | 1398-5647 | 1399-5618 | 26 | SCIE | CLINICAL NEUROLOGY;NEUROSCIENCES;PSYCHIATRY | 2024 | 4.5 | 14.4 | 0 | English | 2024 | 2024-09 | 바로가기 | 바로가기 | |||||||||||||||||
| ○ | ○ | Article | Editing of the ethylene biosynthesis gene in carnation using CRISPR-Cas9 ribonucleoprotein complex | The study aimed to edit ethylene (ET) biosynthesis genes [1-aminocyclopropane-1-carboxylic acid (ACC) synthetase 1 (ACS1) and ACC oxidase 1 (ACO1)] in carnation using the CRISPR/Cas9 ribonucleoprotein (RNP) complex system. Initially, the conserved regions of the target genes (ACS1 and ACO1) were validated for the generation of different single guide RNAs (sgRNAs), followed by the use of an in vitro cleavage assay to confirm the ability of the sgRNAs to cleave the target genes specifically. The in vitro cleavage assay revealed that the sgRNAs were highly effective in cleaving their respective target regions. The complex of sgRNA: Cas9 was directly delivered into the carnation protoplast, and the target genes in the protoplast were deep-sequenced. The results revealed that the sgRNAs were applicable for editing the ET biosynthesis genes, as the mutation frequency ranged from 8.8 to 10.8% for ACO1 and 0.2-58.5% for ACS1. When sequencing the target genes in the callus derived from the protoplasts transformed with sgRNA: Cas9, different indel patterns (+ 1, - 1, and - 8 bp) in ACO1 and (- 1, + 1, and + 11) in ACS1 were identified. This study highlighted the potential application of CRISPR/Cas9 RNP complex system in facilitating precise gene editing for ET biosynthesis in carnation. | Adedeji, Oluwaseun Suleimon; Naing, Aung Htay; Kang, Hyunhee; Xu, Junping; Chung, Mi Young; Kim, Chang Kil | Kyungpook Natl Univ, Dept Hort, Daegu 41566, South Korea; Lingnan Normal Univ, Life Sci & Technol Sch, Zhanjiang 524048, Peoples R China; Sunchon Natl Univ, Dept Agr Educ, Sunchon, South Korea | Xu, Junping/LNQ-8618-2024; Adedeji, Oluwaseun/IUN-2788-2023; Naing, Aung Htay/AAF-4277-2019 | 57216153720; 37112768100; 57217681910; 56677024300; 24821361600; 7409880701 | aunghtaynaing2005@gmail.com;ckkim@knu.ac.kr; | PLANT METHODS | PLANT METHODS | 1746-4811 | 20 | 1 | SCIE | BIOCHEMICAL RESEARCH METHODS;PLANT SCIENCES | 2024 | 4.4 | 14.5 | 1.4 | 2025-05-07 | 2 | 3 | Calli; CRISPR/Cas9; Ethylene biosynthesis genes; Indel patterns; In vitro cleavage; Protoplast | PROTOPLASTS; SYSTEM; SENESCENCE; OPTIMIZATION; REGENERATION; GYNOECIUM; FLOWERS; SILVER; PLANTS | Calli; CRISPR/Cas9; Ethylene biosynthesis genes; In vitro cleavage; Indel patterns; Protoplast | English | 2024 | 2024-02-02 | 10.1186/s13007-024-01143-0 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Evacuation information methodology that combined a flooded environment and pedestrian behavioral model | Disaster information influences decision-making. Wireless internet and location-based services, aided by recent technological advances, have laid the foundation for providing personalized disaster information. Despite the provision of simple disaster forecasts and warnings, provision of specific evacuation actions ("where" and "how," i.e., location and reachability of safe areas) and location-based personalized information that consider real-time disaster situations are a pressing priority. Thus, this study proposed and developed a three-step methodology for providing disaster information during floods that combined a flooded environment and pedestrian behavioral model, namely, 1) prediction of the flooded environment, 2) analysis of evacuation routes based on the movement time of evacuees according to the flood depth, and 3) provision of evacuation information by selecting the optimal evacuation route. This model was demonstrated in the Gangnam Station area in Seoul, Korea. Flooded environment prediction modeling of the target area was performed by applying the 100-year rainfall design to the Storm Water Management Model. The optimal evacuation route, according to the flood depth, was selected by applying the behavioral model (movement time calculation formula) for each evacuee derived through an evacuation behavior experiment. The pre- and post-flooding age- and gender-wise total evacuation distance and time were compared and analyzed. Post-flooding, the evacuation distance and time increased by 168.3% (381.34 m) and 172.3% (3.16 min) on average, respectively, indicating that evacuees must travel farther and longer. The analysis of post-flooding evacuation route change types revealed changes in evacuation distance and time in cases where shelters and evacuation routes and time were significantly different depending on evacuee characteristics (age and gender). This indicated that specific and visual disaster information can be provided according to evacuees' age and gender using the proposed methodology. This study can serve as the basis for designing an advanced and personalized disaster information system that can provide specific evacuation actions to aid fast evacuation decision-making during floods. | Lee, Hye-Kyoung; Son, Byeung-Hun; Kim, Young-Chan; Hong, Won-Hwa | Kyungpook Natl Univ, Architecture Inst A3, Daegu 41566, South Korea; Daegu Tech Univ, Dept Architecture, Daegu 704721, South Korea; Dongguk Univ, Div Smart Safety Engn, WISE, Gyeongju 38066, South Korea; Kyungpook Natl Univ, Sch Architectural Civil Environm & Energy Engn, Daegu 41566, South Korea | 57209320452; 56909550800; 56463201400; 7401527968 | hongwonhwa@gmail.com; | INTERNATIONAL JOURNAL OF DISASTER RISK REDUCTION | INT J DISAST RISK RE | 2212-4209 | 106 | SCIE | GEOSCIENCES, MULTIDISCIPLINARY;METEOROLOGY & ATMOSPHERIC SCIENCES;WATER RESOURCES | 2024 | 4.5 | 14.5 | 0 | 2025-05-07 | 0 | 0 | Disaster risk reduction; Disaster resilience; Disaster information; Flood evacuation; Pedestrian behavioral model | Disaster information; Disaster resilience; Disaster risk reduction; Flood evacuation; Pedestrian behavioral model | English | 2024 | 2024-05 | 10.1016/j.ijdrr.2024.104438 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||||
| ○ | ○ | Article | Production of genetically stable and Odontoglossum ringspot virus-free Cymbidium orchid 'New True' plants via meristem-derived protocorm-like body (PLB) subcultures | Background This study aimed to produce Odontoglossum ringspot virus (ORSV)-free Cymbidium orchid 'New True' plants from ORSV-infected mother plants by culturing their meristems and successively repeating subcultures of protocorm-like bodies (PLBs) derived from the meristems. Results Initially, ORSV was confirmed as the causative agent of viral symptoms in orchid leaves via reverse transcription-polymerase chain reaction (RT-PCR) analysis. Meristems from infected plants were cultured to generate PLBs, which in sequence were repeatedly subcultured up to four times. RT-PCR and quantitative RT-PCR analyses revealed that while ORSV was undetectable in shoots derived from the first subculture, complete elimination of the virus required at least a second subculture. Genetic analysis using inter-simple sequence repeat markers indicated no somaclonal variation between regenerated plants and the mother plant, suggesting that genetic consistency was maintained. Conclusion Overall, our findings demonstrate that subculturing PLBs for a second time is ideal for producing genetically stable, ORSV-free Cymbidium orchids, thus offering a practical means of generating genetically stable, virus-free plants and enhancing plant health and quality in the orchid industry. | Campol, Jova Riza; Naing, Aung Htay; Aung, Hay Mon; Cho, Su Bin; Kang, Hyunhee; Chung, Mi Young; Kim, Chang Kil | Kyungpook Natl Univ, Dept Hort Sci, Daegu, South Korea; Sunchon Natl Univ, Dept Agr Educ, Sunchon, South Korea | Naing, Aung Htay/AAF-4277-2019 | 57486378900; 37112768100; 59335761100; 59335432900; 57217681910; 24821361600; 7409880701 | aunghtaynaing2005@gmail.com;ckkim@knu.ac.kr; | PLANT METHODS | PLANT METHODS | 1746-4811 | 20 | 1 | SCIE | BIOCHEMICAL RESEARCH METHODS;PLANT SCIENCES | 2024 | 4.4 | 14.5 | 0.93 | 2025-05-07 | 4 | 3 | Cymbidium orchid; Genetic stability; Meristem culture; ISSR marker; qRT-PCR analysis; Protocorm-like bodies (PLBs) | MOSAIC-VIRUS; ISSR; PHALAENOPSIS; ELIMINATION; FIDELITY; RAPD | Cymbidium orchid; Genetic stability; ISSR marker; Meristem culture; Protocorm-like bodies (PLBs); qRT-PCR analysis | English | 2024 | 2024-09-19 | 10.1186/s13007-024-01269-1 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Actin depolymerizing factor destrin governs cell migration in neural development during Xenopus embryogenesis | The actin -based cytoskeleton is considered a fundamental driving force for cell differentiation and development. Destrin (Dstn), a member of the actin-depolymerizing factor family, regulates actin dynamics by treadmilling actin filaments and increasing globular actin pools. However, the specific developmental roles of dstn have yet to be fully elucidated. Here, we investigated the physiological functions of dstn during early embryonic development using Xenopus laevis as an experimental model organism. dstn is expressed in anterior neural tissue and neural plate during Xenopus embryogenesis. Depleting dstn promoted morphants with short body axes and small heads. Moreover, dstn inhibition extended the neural plate region, impairing cell migration and distribution during neurulation. In addition to the neural plate, dstn knockdown perturbed neural crest cell migration. Our data suggest new insights for understanding the roles of actin dynamics in embryonic neural development, simultaneously presenting a new challenge for studying the complex networks governing cell migration involving actin dynamics. (c) 2024 The Author(s). Published by Elsevier Inc. on behalf of Korean Society for Molecular and Cellular Biology. This is an open access article under the CC BY -NC -ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | Kim, Youni; Lee, Hyun-Kyung; Park, Kyeong-Yeon; Ismail, Tayaba; Lee, Hongchan; Ryu, Hong-Yeoul; Cho, Dong-Hyung; Kwon, Taeg Kyu; Park, Tae Joo; Kwon, Taejoon; Lee, Hyun-Shik | Kyungpook Natl Univ, Coll Nat Sci, Sch Life Sci, KNU G LAMP Project Grp,KNU Inst Basic Sci,FOUR KNU, Daegu 41566, South Korea; Keimyung Univ, Sch Med, Dept Immunol, Daegu 41566, South Korea; Ulsan Natl Inst Sci & Technol UNIST, Coll Informat Bio Convergence, Dept Biol Sci, Ulsan 44919, South Korea; Ulsan Natl Inst Sci & Technol UNIST, Dept Biomed Engn, Coll Informat Bio Convergence Engn, Ulsan 44919, South Korea | Choi, Hye Rin/JDV-9065-2023; Kwon, Taejoon/AAR-9814-2020; Ismail, Tayaba/LPQ-9181-2024; Lee, Han Hee/AAB-7987-2022; Park, Tae Joo/G-1460-2011 | 57204943696; 57221571155; 57190389243; 57190277495; 57210948333; 55889917800; 35093684400; 7202206057; 58254025900; 35484365800; 16316010200 | leeh@knu.ac.kr; | MOLECULES AND CELLS | MOL CELLS | 1016-8478 | 0219-1032 | 47 | 6 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CELL BIOLOGY | 2024 | 6.5 | 14.6 | 1.24 | 2025-05-07 | 3 | 3 | N-COFILIN; ADF/COFILIN; NEURULATION; PROTEINS; DYNAMICS; ORIGIN; RODS | Destrin; F-actin; Neural crest; Neurulation; Xenopus laevis | Actins; Animals; Cell Movement; Destrin; Embryonic Development; Gene Expression Regulation, Developmental; Neural Crest; Neural Plate; Neurogenesis; Xenopus laevis; Xenopus Proteins; actin depolymerizing factor; actin; actin depolymerizing factor; Xenopus protein; adult; animal cell; animal experiment; animal model; Article; cell migration; cellular distribution; confocal microscopy; controlled study; embryo development; enzyme inhibition; gene knockdown; immunofluorescence assay; in situ hybridization; nerve cell differentiation; nervous tissue; neural crest cell; neuroectoderm; neurulation; nonhuman; plasmid; protein depletion; protein expression; real time polymerase chain reaction; Western blotting; Xenopus laevis; animal; cell motion; cytology; embryology; gene expression regulation; genetics; metabolism; nervous system development; neural crest; Xenopus laevis | English | 2024 | 2024-06 | 10.1016/j.mocell.2024.100076 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | An Empirical Analysis of Factors Affecting OTT Service Users' Switching Intention: Focusing on Netflix and the Perspective of the Push-Pull-Mooring Framework | In a situation where competition in the OTT service market intensifies, this study analyzed the factors affecting the switching behavior of OTT service users using the push-pull-mooring (PPM) model. This study presented push factors (perceived risk and low satisfaction), pull factors (attractiveness of alternatives and content richness), and mooring factors (switching cost and brand familiarity) based on the main PPM factors affecting the switching behavior of existing online services. The results showed that low satisfaction, perceived risk, the attractiveness of alternatives, and content richness had positive effects on switching intention. Additionally, switching costs and brand familiarity had a negative effect on switching intention, and these effects were moderated by habit. This study adds to the literature by broadly explaining users' behavior toward OTT services using the PPM model. Furthermore, our findings will benefit corporate managers providing OTT services. | Yoon, Jae Hyun; Kim, Han Ku | Kyungpook Natl Univ, Sch Business Adm, Daegu, South Korea | 57300753800; 57195427228 | hangu19@knu.ac.kr; | INTERNATIONAL JOURNAL OF HUMAN-COMPUTER INTERACTION | INT J HUM-COMPUT INT | 1044-7318 | 1532-7590 | 40 | 12 | SCIE;SSCI | COMPUTER SCIENCE, CYBERNETICS;ERGONOMICS | 2024 | 4.9 | 14.6 | 3.32 | 2025-04-16 | 10 | 15 | OTT service; push-pull-mooring model; perceived risk; low satisfaction; attractiveness of alternatives; content richness; switching cost; brand familiarity; habit; switching intention | PERCEIVED RISK; ONLINE; MIGRATION; SATISFACTION; DIMENSIONS; KNOWLEDGE; LOYALTY; MODEL | attractiveness of alternatives; brand familiarity; content richness; habit; low satisfaction; OTT service; perceived risk; push-pull-mooring model; switching cost; switching intention | Cost benefit analysis; Risk assessment; Attractiveness of alternative;; Brand familiarity;; Content richness;; Habit;; Low satisfaction;; OTT service;; Perceived risk; Perceived risk;; Push pull; Push-pull-mooring model;; Switching cost;; Switching costs; Switching intentions; Mooring | English | 2024 | 2024-06-17 | 10.1080/10447318.2023.2185732 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | Article | Development of a Viewing-Purchase Model for Live Streaming Commerce: A Social Exchange Perspective | Live streaming commerce has rapidly emerged as a key platform in e-commerce, yet the socio-technical mechanisms underlying consumer-platform interactions remain underexplored. This study addresses this gap by examining both task-oriented purchasing behaviors and pleasure-driven engagement through the lens of social exchange theory. A web-based survey was conducted with 300 respondents in South Korea, all of whom had prior experience with live streaming commerce. The data were analyzed using PLS-SEM to evaluate the proposed model. The results reveal that procedural and interactional justice positively affect trust, while co-experience enhances perceived playfulness. Both trust and playfulness, in turn, significantly impact purchase and repeated viewing intentions. These findings provide valuable insights into the dynamics of live commerce, offering a comprehensive model for understanding consumer behavior in the context of live commerce. © 2024 Taylor & Francis Group, LLC. | Choi, Hanbyul; Kim, Junghwan; Park, Jonghwa | Department of Management Information System, Dong-A University, Busan, South Korea; Department of Mass Communication, Pukyong National University, Busan, South Korea; School of Business Administration, Kyungpook National University, Daegu, South Korea | 57199053804; 57211825131; 59388235000 | jonghwapark@knu.ac.kr; | International Journal of Human-Computer Interaction | INT J HUM-COMPUT INT | 1044-7318 | 1532-7590 | SCIE;SSCI | COMPUTER SCIENCE, CYBERNETICS;ERGONOMICS | 2024 | 4.9 | 14.6 | 0 | 2025-05-07 | 0 | co-experience; justice; Live streaming commerce; social exchange theory; stimuli–organism-response framework | Sales; Co experiences; E- commerces; Justice; Live streaming; Live streaming commerce; Social exchange; Social exchange theory; Sociotechnical; Stimulus–organism-response framework; Task-oriented; Marketplaces | English | Article in press | 2024 | 10.1080/10447318.2024.2427901 | 바로가기 | 바로가기 | 바로가기 | |||||||||
| ○ | ○ | Article | Second derivative Lδ-estimates for a class of singular fully nonlinear elliptic equations | We provide global a priori second derivative L-delta-estimates for a class of singular fully nonlinear elliptic equations with right hand side terms of L-n. | Baasandorj, Sumiya; Byun, Sun-Sig; Oh, Jehan | Scuola Normale Super Pisa, Piazza Cavalieri 7, I-56126 Pisa, Italy; Seoul Natl Univ, Dept Math Sci, Seoul 08826, South Korea; Seoul Natl Univ, Res Inst Math, Seoul 08826, South Korea; Kyungpook Natl Univ, Dept Math, Daegu 41566, South Korea | 57216991620; 8350569400; 56673305300 | sumiya.baasandorj@snt.it;byun@snu.ac.kr;jehan.oh@knu.ac.kr; | NONLINEAR ANALYSIS-THEORY METHODS & APPLICATIONS | NONLINEAR ANAL-THEOR | 0362-546X | 1873-5215 | 249 | SCIE | MATHEMATICS, APPLIED;MATHEMATICS | 2024 | 1.3 | 14.6 | 0.76 | 2025-05-07 | 2 | 2 | Singular fully nonlinear equations; Global second derivative L-delta-estimates; Viscosity solutions | PARTIAL REGULARITY; GENERAL-CLASS; GRADIENT | Global second derivative L<sup>δ</sup>-estimates; Singular fully nonlinear equations; Viscosity solutions | Nonlinear analysis; Fully nonlinear elliptic equations; Fully nonlinear equations; Global second derivative Lδ-estimate; Right-hand sides; Second derivatives; Singular fully nonlinear equation; Viscosity solutions; Nonlinear equations | English | 2024 | 2024-12 | 10.1016/j.na.2024.113630 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Targeting of CYP2E1 by miRNAs in alcohol-induced intestine injury | Although binge alcohol-induced gut leakage has been studied extensively in the context of reactive oxygen species-mediated signaling, it was recently revealed that post-transcriptional regulation plays an essential role as well. Ethanol (EtOH)-inducible cytochrome P450-2E1 (CYP2E1), a key enzyme in EtOH metabolism, promotes alcohol-induced hepatic steatosis and inflammatory liver disease, at least in part by mediating changes in intestinal permeability. For instance, gut leakage and elevated intestinal permeability to endotoxins have been shown to be regulated by enhancing CYP2E1 mRNA and CYP2E1 protein levels. Although it is understood that EtOH promotes CYP2E1 induction and activation, the mechanisms that regulate CYP2E1 expression in the context of intestinal damage remain poorly defined. Specific miRNAs, including miR-132, miR-212, miR-378, and miR-552, have been shown to repress the expression of CYP2E1, suggesting that these miRNAs contribute to EtOH-induced intestinal injury. Here, we have shown that CYP2E1 expression is regulated post-transcriptionally through miRNA-mediated degradation, as follows: (1) the RNA-binding protein AU- binding factor 1 (AUF1) binds mature miRNAs, including CYP2E1-targeting miRNAs, and this binding modulates the degradation of corresponding target mRNAs upon EtOH treatment; (2) the serine/threonine kinase mammalian Ste20-like kinase 1 (MST1) mediates oxidative stress-induced phosphorylation of AUF1. Those findings suggest that reactive oxygen species-mediated signaling modulates AUF1/miRNA interaction through MST1-mediated phosphorylation. Thus, our study demonstrates the critical functions of AUF1 phosphorylation by MST1 in the decay of miRNAs targeting CYP2E1, the stabilization of CYP2E1 mRNA in the presence of EtOH, and the relationship of this pathway to subsequent intestinal injury. (c) 2024 Published by Elsevier Inc. on behalf of Korean Society for Molecular and Cellular Biology. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | Mun, Hyejin; Lee, Sungyul; Choi, Suyoung; Jeong, Ji-Hoon; Ko, Seungbeom; Chun, Yoo Lim; Deaton, Benjamin; Yeager, Clay T.; Boyette, Audrey; Palmera, Juliana; Newman, London; Zhou, Ping; Shin, Soona; Kim, Dong-Chan; Sagum, Cari A.; Bedford, Mark T.; Kim, Young-Kook; Kwon, Jaeyul; Jung, Junyang; Chang, Jeong Ho; Yoon, Je-Hyun | Med Univ South Carolina, Dept Biochem & Mol Biol, Charleston, SC 29425 USA; Univ Oklahoma, Dept Oncol Sci, Oklahoma City, OK 73104 USA; Seoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea; Chungnam Natl Univ, Coll Med, Dept Infect Biol, Daejeon 35015, South Korea; Chungnam Natl Univ, Coll Med, Dept Med Sci, Daejeon 35015, South Korea; Chungnam Natl Univ, Brain Korea FOUR Project Med Sci 21, Daejeon 35015, South Korea; Kyung Hee Univ, Coll Med, Dept Anat & Neurobiol, 26 Kyungheedae Ro, Seoul 02447, South Korea; Cincinnati Childrens Hosp Med Ctr, Div Pediat Gen & Thorac Surg, Cincinnati, OH 45229 USA; Univ Cincinnati, Coll Med, Dept Surg, Cincinnati, OH 45267 USA; NQ Lab Inc, Div Med Device, R&D Ctr, Yongin 16827, Gyeonggi Do, South Korea; Univ Texas MD Anderson Canc Ctr, Dept Epigenet & Mol Carcinogenesis, Houston, TX 77030 USA; Chonnam Natl Univ, Med Sch, Dept Biochem, Hwasun 58128, South Korea; Chungnam Natl Univ, Coll Med, Dept Med Educ, Daejeon 35015, South Korea; Chungnam Natl Univ, Translat Immunol Inst, Daejeon 35015, South Korea; Kyungpook Natl Univ, Dept Biol Educ, Daegu 41566, South Korea | ; Shin, Soona/AAV-1049-2021; Song, Juhyun/AAH-3162-2020 | 57216672802; 56822590500; 57223209592; 57200007356; 57212308322; 57212409596; 59194774900; 59195155000; 59194411900; 59194963200; 59194775000; 57216361287; 55459493700; 57200217505; 55602083500; 7006206437; 57208862490; 7202469129; 55335086500; 57203598905; 55318967100 | jjung@khu.ac.kr;jhcbio@knu.ac.kr;jehyun-yoon@ouhsc.edu; | MOLECULES AND CELLS | MOL CELLS | 1016-8478 | 0219-1032 | 47 | 7 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CELL BIOLOGY | 2024 | 6.5 | 14.6 | 0.83 | 2025-05-07 | 4 | 5 | AU-binding factor 1; Intestine; microRNA; Mammalian Ste20-like kinase 1 | STEM-CELLS; INHIBITION; LIVER; ACTIVATION; AUF1; PHOSPHORYLATION; TRANSLATION; SUPPRESSES; RNAS | AU-binding factor 1; Cytochrome P450-2E1; Intestine; Mammalian Ste20-like kinase 1; microRNA | Animals; Cytochrome P-450 CYP2E1; Ethanol; Heterogeneous Nuclear Ribonucleoprotein D0; Humans; Intestinal Mucosa; Intestines; MicroRNAs; 4 aminobutyric acid; circular ribonucleic acid; clomethiazole; cytochrome P450 2E1; endotoxin; fibrinogen; heterogeneous nuclear ribonucleoprotein D0; messenger RNA; microRNA 132; protein serine threonine kinase; reactive oxygen metabolite; RNA binding protein; alcohol; HNRNPD protein, human; microRNA; adult; animal model; animal tissue; Article; cell culture; controlled study; crypt cell; degradation; fatty liver; human; human cell; intestinal organoid; intestine crypt; intestine epithelium cell; Lieber DeCarli diet; mouse; nonhuman; oxidative stress; phosphorylation; RNA translation; RNA-binding domain; small intestine; T84 cell line; upregulation; animal; genetics; intestine; intestine mucosa; metabolism; pathology | English | 2024 | 2024-07 | 10.1016/j.mocell.2024.100074 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Review | Unlocking biological mechanisms with integrative functional genomics approaches | Reverse genetics offers precise functional insights into genes through the targeted manipulation of gene expression followed by phenotypic assessment. While these approaches have proven effective in model organisms such as Saccharomyces cerevisiae, large-scale genetic manipulations in human cells were historically unfeasible due to methodological limitations. However, recent advancements in functional genomics, particularly clustered regularly interspaced short palindromic repeats (CRISPR)-based screening technologies and next-generation sequencing platforms, have enabled pooled screening technologies that allow massively parallel, unbiased assessments of biological phenomena in human cells. This review provides a comprehensive overview of cutting-edge functional genomic screening technologies applicable to human cells, ranging from short hairpin RNA screens to modern CRISPR screens. Additionally, we explore the integration of CRISPR platforms with single-cell approaches to monitor gene expression, chromatin accessibility, epigenetic regulation, and chromatin architecture following genetic perturbations at the omics level. By offering an indepth understanding of these genomic screening methods, this review aims to provide insights into more targeted and effective strategies for genomic research and personalized medicine. (c) 2024 The Author(s). Published by Elsevier Inc. on behalf of Korean Society for Molecular and Cellular Biology. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | Yun, Sehee; Noh, Minsoo; Yu, Jivin; Kim, Hyeon-Jai; Hui, Chi-Chung; Lee, Hunsang; Son, Joe Eun | Korea Univ, Dept Life Sci, Seoul 02841, South Korea; Gachon Univ, Dept Internal Med, Coll Med, Incheon 21565, South Korea; Gachon Univ, Coll Med, Lab Genom & Translat Med, Incheon 21565, South Korea; Hosp Sick Children, Program Dev & Stem Cell Biol, Toronto, ON, Canada; Univ Toronto, Dept Mol Genet, Toronto, ON, Canada; Kyungpook Natl Univ, Sch Food Sci & Biotechnol, Daegu 41566, South Korea | 58814859100; 57194002667; 58814552900; 58814449700; 7202876913; 59859433500; 35362802300 | joeson@knu.ac.kr;hunsang@korea.ac.kr; | MOLECULES AND CELLS | MOL CELLS | 1016-8478 | 0219-1032 | 47 | 8 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CELL BIOLOGY | 2024 | 6.5 | 14.6 | 0.18 | 2025-05-07 | 1 | 2 | Clustered regularly interspaced short palindromic repeats screening; Functional genomics; Genome-wide screening; Reverse genetics; Single-cell genomics; Systems biology | RNA-INTERFERENCE; CHROMATIN ACCESSIBILITY; REVEALS PRINCIPLES; REVERSE GENETICS; SHRNA LIBRARIES; ESSENTIAL GENES; HUMAN-CELLS; SINGLE; CRISPR; IDENTIFICATION | Clustered regularly interspaced short palindromic repeats screening; Functional genomics; Genome-wide screening; Reverse genetics; Single-cell genomics; Systems biology | Chromatin; CRISPR-Cas Systems; Epigenesis, Genetic; Genomics; High-Throughput Nucleotide Sequencing; Humans; short hairpin RNA; cellular, subcellular and molecular biological phenomena and functions; chromatin; clustered regularly interspaced short palindromic repeat; drug analysis; functional genomics; gene expression; genetic manipulation; genomics; high throughput sequencing; human; human cell; nonhuman; personalized medicine; phenotype; reverse genetics; review; Saccharomyces cerevisiae; systems biology; CRISPR Cas system; genetic epigenesis; genetics; metabolism; procedures | English | 2024 | 2024-08 | 10.1016/j.mocell.2024.100092 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | AMPK-Mediated Hypolipidemic Effects of a Salvia miltiorrhiza and Paeonia lactiflora Mixed Extract on High-Fat Diet-Induced Liver Triglyceride Accumulation: An In Vivo and In Vitro Study | Background: This study investigates the hypolipidemic effects of a mixed extract of Salvia miltiorrhiza and Paeonia lactiflora (USCP119) in HFD-fed hamsters and in vitro cellular models. Methods: Over an 8-week period, HFD-fed hamsters were assigned to one of six groups: normal diet, HFD control, HFD with 50 mg/kg USCP119, HFD with 100 mg/kg USCP119, HFD with 50 mg/kg USCP119 twice daily (BID), and HFD with omega-3 fatty acids. Key outcomes assessed included body weight, serum triglycerides (TG), total cholesterol (TC), liver weight, hepatic TG levels, and epididymal fat. In cellular models, the impact of USCP119 on lipid accumulation and adipogenic markers was evaluated. Results: USCP119 treatment at 50 mg/kg BID resulted in the lowest weight gain (15.5%) and the most significant reductions in serum TG and hepatic TG levels compared to the HFD control. The 100 mg/kg dose also led to substantial reductions in serum TG and TC levels and notable decreases in low-density lipoprotein cholesterol. USCP119 at 50 mg/kg once daily reduced TG and TC levels but was less effective than the higher doses. In cellular models, USCP119 was non-toxic up to 400 mu g/mL and effectively reduced lipid accumulation, modulated adipogenic markers, and enhanced AMPK signaling, improving lipid metabolism and insulin sensitivity. Conclusions: All USCP119 treatments demonstrated effectiveness in managing hyperlipidemia and related metabolic disorders, with variations in impact depending on the dosage. The ability of USCP119 to reduce fat accumulation, improve lipid profiles, and enhance insulin sensitivity highlights its potential as a valuable dietary supplement for addressing high-fat diet-induced hyperlipidemia and metabolic disturbances. | Son, Juah; Phong, Nguyen Viet; Cha, Mi-Ran; Oh, Byulnim; Song, Sukjin; Yang, Seo Young | USCAREPHARM Co Ltd, Seoul 16690, South Korea; Kyungpook Natl Univ, Teachers Coll, Dept Biol Educ, Daegu 41566, South Korea; Kyungpook Natl Univ, Inst Phylogen & Evolut, Daegu 41566, South Korea | Nguyen, Viet Phong/AAQ-4207-2021 | 58894533400; 57205196739; 58894665600; 58965102700; 58894275300; 57215036954 | juah@uscarepharm.com;ngvietphong@gmail.com;mrcha@uscarepharm.com;aster@uscarepharm.com;uscare@uscarepharm.com;syy@knu.ac.kr; | NUTRIENTS | NUTRIENTS | 2072-6643 | 16 | 18 | SCIE | NUTRITION & DIETETICS | 2024 | 5 | 14.7 | 0 | 2025-05-07 | 0 | 0 | Salvia miltiorrhiza; Paeonia lactiflora; triglyceride accumulation; hypolipidemic effects; high-fat diet; hepatic steatosis; AMPK | LIPID-METABOLISM; INFLAMMATION | AMPK; hepatic steatosis; high-fat diet; hypolipidemic effects; Paeonia lactiflora; Salvia miltiorrhiza; triglyceride accumulation | AMP-Activated Protein Kinases; Animals; Cricetinae; Diet, High-Fat; Humans; Hyperlipidemias; Hypolipidemic Agents; Lipid Metabolism; Liver; Male; Mesocricetus; Paeonia; Plant Extracts; Salvia miltiorrhiza; Triglycerides; antilipemic agent; cholesterol; hydroxymethylglutaryl coenzyme A reductase kinase kinase; low density lipoprotein cholesterol; omega 3 fatty acid; triacylglycerol; unclassified drug; uscp 119; antilipemic agent; hydroxymethylglutaryl coenzyme A reductase kinase; plant extract; triacylglycerol; adipogenesis; AMPK signaling; animal cell; animal experiment; animal model; animal tissue; Article; body weight; body weight gain; cholesterol blood level; controlled study; drug dose comparison; drug megadose; enzyme inhibition; enzyme phosphorylation; epididymis fat; hamster model; human; human cell; hyperlipidemia; hypolipidemic activity; in vitro study; in vivo study; insulin sensitivity; lipid diet; lipid metabolism; lipid storage; liver weight; low drug dose; male; mouse; nonalcoholic fatty liver; nonhuman; outcome assessment; triacylglycerol blood level; adverse event; animal; blood; chemistry; drug effect; drug therapy; hamster; hyperlipidemia; liver; Mesocricetus; metabolism; Paeonia; Salvia miltiorrhiza | English | 2024 | 2024-09 | 10.3390/nu16183189 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | Article | Enhancing the Efficiency and Stability of Tin-Lead Perovskite Solar Cells via Sodium Hydroxide Dedoping of PEDOT:PSS | Tin-lead (Sn-Pb) perovskite solar cells (PSCs) have gained interest as candidates for the bottom cell of all-perovskite tandem solar cells due to their broad absorption of the solar spectrum. A notable challenge arises from the prevalent use of the hole transport layer, PEDOT:PSS, known for its inherently high doping level. This high doping level can lead to interfacial recombination, imposing a significant limitation on efficiency. Herein, NaOH is used to dedope PEDOT:PSS, with the aim of enhancing the efficiency of Sn-Pb PSCs. Secondary ion mass spectrometer profiles indicate that sodium ions diffuse into the perovskite layer, improving its crystallinity and enlarging its grains. Comprehensive evaluations, including photoluminescence and nanosecond transient absorption spectroscopy, confirm that dedoping significantly reduces interfacial recombination, resulting in an open-circuit voltage as high as 0.90 V. Additionally, dedoping PEDOT:PSS leads to increased shunt resistance and high fill factor up to 0.81. As a result of these improvements, the power conversion efficiency is enhanced from 19.7% to 22.6%. Utilizing NaOH to dedope PEDOT:PSS also transitions its nature from acidic to basic, enhancing stability and exhibiting less than a 7% power conversion efficiency loss after 1176 h of storage in N2 atmosphere. © 2024 Wiley-VCH GmbH. | Wu, Dong-Tai; Zhu, Wen-Xian; Dong, Yueyao; Daboczi, Matyas; Ham, Gayoung; Hsieh, Hsing-Jung; Huang, Chi-Jing; Xu, Weidong; Henderson, Charlie; Kim, Ji-Seon; Eslava, Salvador; Cha, Hyojung; Macdonald, Thomas J.; Lin, Chieh-Ting | Department of Chemical Engineering, National Chung Hsing University, 145 Xingda Road, Taichung, 402–27, Taiwan; Department of Chemical Engineering, National Chung Hsing University, 145 Xingda Road, Taichung, 402–27, Taiwan; School of Engineering and Materials Science, Queen Mary University of London, London, E1 4NS, United Kingdom; Department of Chemical Engineering and Centre for Processable Electronics, Imperial College London, London, SW7 2AZ, United Kingdom; Department of Energy Convergence and Climate Change, Kyungpook National University, Daegu, 41566, South Korea; Department of Chemical Engineering, National Chung Hsing University, 145 Xingda Road, Taichung, 402–27, Taiwan; Department of Chemical Engineering, National Chung Hsing University, 145 Xingda Road, Taichung, 402–27, Taiwan; Department of Chemical Engineering and Biotechnology, University of Cambridge, Philippa Fawcett Drive, Cambridge, CB3 0AS, United Kingdom; Department of Physics and Centre for Processable Electronics, Imperial College London, London, SW7 2AZ, United Kingdom; Department of Physics and Centre for Processable Electronics, Imperial College London, London, SW7 2AZ, United Kingdom; Department of Chemical Engineering and Centre for Processable Electronics, Imperial College London, London, SW7 2AZ, United Kingdom; Department of Energy Convergence and Climate Change, Kyungpook National University, Daegu, 41566, South Korea, Department of Hydrogen and Renewable Energy, Kyungpook National University, Daegu, 41566, South Korea; School of Engineering and Materials Science, Queen Mary University of London, London, E1 4NS, United Kingdom; Department of Chemical Engineering, National Chung Hsing University, 145 Xingda Road, Taichung, 402–27, Taiwan, Innovation and Development Center of Sustainable Agriculture, National Chung Hsing University, Taichung, 402, Taiwan | 58764377800; 58934562100; 59281260300; 56868636700; 58506766600; 58764377900; 58934711500; 57222284706; 57357181500; 55757743100; 22834486800; 35885242200; 56210619300; 57194687776 | c.lin15@nchu.edu.tw; | Small Methods | SMALL METHODS | 2366-9608 | 2366-9608 | 8 | 12 | SCIE | CHEMISTRY, PHYSICAL;MATERIALS SCIENCE, MULTIDISCIPLINARY;NANOSCIENCE & NANOTECHNOLOGY | 2024 | 9.1 | 14.7 | 1.1 | 2025-05-07 | 6 | charge carrier recombination; doping; narrow-bandgap perovskite; pedot:pss; sn-pb perovskite | Absorption spectroscopy; Conducting polymers; Conversion efficiency; Crystallinity; Metal ions; Open circuit voltage; Perovskite solar cells; Sodium hydroxide; Spectrometers; Tin; perovskite; sodium hydroxide; sodium ion; tin; Bottom cells; Charge carrier recombination; De-doping; High doping level; Narrow bandgap; Narrow-bandgap perovskite; PEDOT/PSS; Power conversion efficiencies; Sn-pb perovskite; Tin-lead; absorption; article; controlled study; electric potential; genetic recombination; mass spectrometer; pharmaceutics; photoluminescence; solar cell; transient absorption spectroscopy; Perovskite | English | Final | 2024 | 10.1002/smtd.202400302 | 바로가기 | 바로가기 | 바로가기 | |||||||
| ○ | ○ | Article | Euglena gracilis Enhances Innate and Adaptive Immunity through Specific Expression of Dectin-1 in CP-Induced Immunosuppressed Mice | Background: Euglena gracilis (E. gracilis), a species of unicellular algae, can accumulate large amounts of beta-1,3-glucan paramylon, a polysaccharide, in its cytoplasm and has recently attracted interest as a bioproduct due to its various health benefits. In this study, the immune-enhancing effect of E. gracilis powder (EP) was investigated in vitro and in vivo. Methods: In vitro, the production of NO and cytokines and the mechanism of the signaling pathway of beta-1,3-glucan were identified in RAW264.7 cells. In vivo, cyclophosphamide-induced (CP-induced) immunosuppressed C57BL/6 female mice were orally administered with three different concentrations (100, 300, and 600 mg/kg) of EP daily. After 14 days, the organs and whole blood were collected from each animal for further study. Results: The weight loss of CP-treated mice was reversed by treatment with EP to levels comparable to those of control mice. In addition, the frequencies of NK1.1(+), CD3(+), CD4(+), CD8(+), and B220(+) in immune cells isolated from the spleen were increased by EP treatment compared with water or RG. The secretion of TNF-alpha, IFN-gamma, and IL-12 from splenocytes was also increased by EP treatment, as was the level of IgM in the serum of the mice. Finally, EP treatment specifically upregulated the expression of dectin-1 in the liver of CP-treated mice. Conclusions: E. gracilis could be a good candidate for a natural immune stimulator in the innate and adaptive response by secreting TNF-alpha, IFN-gamma, and IL-12 through stimulating dectin-1 expression on the surface of immune cells. | Lee, Hwan Hee; Seong, Ji-Yeon; Kang, Hyojeung; Cho, Hyosun | Duksung Womens Univ, Coll Pharm, Seoul 01369, South Korea; Duksung Womens Univ, Duksung Innovat Drug Ctr, Seoul 01369, South Korea; Kyungpook Natl Univ, Coll Pharm, Vessel Organ Interact Res Ctr, VOICE MRC,Canc Res Inst, Daegu 41566, South Korea | 56271051000; 59347006000; 8979751700; 55572361200 | oeo3oeo@gmail.com;sjyne@duksung.ac.kr;hkang72@knu.ac.kr;hyosun1102@duksung.ac.kr; | NUTRIENTS | NUTRIENTS | 2072-6643 | 16 | 18 | SCIE | NUTRITION & DIETETICS | 2024 | 5 | 14.7 | 0 | 2025-05-07 | 0 | 0 | E. gracilis; beta-1,3-glucan; immune-enhancing effect; immunosuppressed mice; dectin-1; cyclophosphamide | NATURAL-KILLER-CELLS; BETA-GLUCAN; PARAMYLON; RECOGNITION; IMMUNOMODULATION; BETA-1,3-GLUCAN; LYMPHOCYTES; MACROPHAGES; INFECTION; CYTOKINE | cyclophosphamide; dectin-1; E. gracilis; immune-enhancing effect; immunosuppressed mice; β-1,3-glucan | Adaptive Immunity; Animals; beta-Glucans; Cyclophosphamide; Cytokines; Euglena gracilis; Female; Glucans; Immunity, Innate; Immunocompromised Host; Interferon-gamma; Interleukin-12; Lectins, C-Type; Mice; Mice, Inbred C57BL; RAW 264.7 Cells; Signal Transduction; Spleen; Tumor Necrosis Factor-alpha; beta 1,3 glucan; CD3 antigen; CD4 antigen; CD8 antigen; cyclophosphamide; dectin 1; Euglena gracilis powder; gamma interferon; immunoglobulin M; immunomodulating agent; interleukin 12; nitric oxide; nk1.1 protein; protein; receptor type tyrosine protein phosphatase C; tumor necrosis factor; unclassified drug; beta glucan; beta-1,3-glucan; cyclophosphamide; cytokine; dectin 1; gamma interferon; glucan; interleukin 12; lectin; paramylon; tumor necrosis factor; adaptive immunity; animal cell; animal experiment; animal tissue; Article; body weight loss; cell isolation; controlled study; cytokine production; drug mechanism; Euglena gracilis; female; immunocompetent cell; immunoglobulin blood level; immunomodulation; in vitro study; in vivo study; innate immunity; mouse; nonhuman; protein expression; RAW 264.7 cell line; signal transduction; spleen cell; upregulation; animal; C57BL mouse; drug effect; immunocompromised patient; immunology; metabolism; spleen | English | 2024 | 2024-09 | 10.3390/nu16183158 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Gynostemma pentaphyllum Hydrodistillate and Its Major Component Damulin B Promote Hair Growth-Inducing Properties In Vivo and In Vitro via the Wnt/β-Catenin Pathway in Dermal Papilla Cells | Alopecia, a prevalent yet challenging condition with limited FDA-approved treatments which is accompanied by notable side effects, necessitates the exploration of natural alternatives. This study elucidated the hair growth properties of Gynostemma pentaphyllum leaf hydrodistillate (GPHD) both in vitro and in vivo. Furthermore, damulin B, a major component of GPHD, demonstrated hair growth-promoting properties in vitro. Beyond its established anti-diabetic, anti-obesity, and anti-inflammatory attributes, GPHD exhibited hair growth induction in mice parallel to minoxidil. Moreover, it upregulated the expression of autocrine factors associated with hair growth, including VEGF, IGF-1, KGF, and HGF. Biochemical assays revealed that minoxidil, GPHD, and damulin B induced hair growth via the Wnt/beta-catenin pathway through AKT signaling, aligning with in vivo experiments demonstrating improved expression of growth factors. These findings suggest that GPHD and damulin B contribute to the hair growth-inducing properties of dermal papilla cells through the AKT/beta-catenin signaling pathway. | Kovale, Lochana; Lee, Seoyeon; Song, Minhyeok; Lee, Jihyun; Son, Hyeong Jig; Sung, Young Kwan; Kwack, Mi Hee; Choe, Wonchae; Kang, Insug; Kim, Sung Soo; Ha, Joohun | Kyung Hee Univ, Coll Med, Grad Sch, Dept Biochem & Mol Biol, Seoul 02447, South Korea; Easy Hydrogen Corp, Jeju City 63196, South Korea; Kyungpook Natl Univ, Sch Med, Dept Immunol, Daegu 41944, South Korea | Ha, Joohun/AAM-1187-2020 | 57355861400; 57216443032; 57215383011; 59095060200; 58805366900; 55663365300; 6507685557; 12243040400; 7203062725; 57981629200; 7202103373 | kovlelochana@gmail.com;wasdde567777@naver.com;thdalsgur77@gmail.com;easyhydrogen@gmail.com;thsgudwlr127@naver.com;ysung@knu.ac.kr;go3004@khu.ac.kr;wchoe@khu.ac.kr;iskang@khu.ac.kr;sgskim@khu.ac.kr;hajh@khu.ac.kr; | NUTRIENTS | NUTRIENTS | 2072-6643 | 16 | 7 | SCIE | NUTRITION & DIETETICS | 2024 | 5 | 14.7 | 1.03 | 2025-05-07 | 2 | 2 | alopecia; Wnt/beta-catenin; Gynostemma pentaphyllum; damulin B | MALE-PATTERN BALDNESS; SIGNALING PATHWAY; MINOXIDIL; ALOPECIA; INDUCTIVITY; DIFFERENTIATION; CLASSIFICATION; IDENTIFICATION; MORPHOGENESIS; MECHANISMS | alopecia; damulin B; Gynostemma pentaphyllum; Wnt/β-catenin | Animals; beta Catenin; Gynostemma; Hair; Mice; Minoxidil; Proto-Oncogene Proteins c-akt; Wnt Signaling Pathway; beta catenin; minoxidil; protein kinase B; animal; Gynostemma; hair; mouse; Wnt signaling | English | 2024 | 2024-04 | 10.3390/nu16070985 | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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