연구성과로 돌아가기
2020 연구성과 (86 / 270)
※ 컨트롤 + 클릭으로 열별 다중 정렬 가능합니다.
Excel 다운로드
| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ○ | ○ | Article | Lablab purpureus Protects HaCaT Cells from Oxidative Stress-Induced Cell Death through Nrf2-Mediated Heme Oxygenase-1 Expression via the Activation of p38 and ERK1/2 | Ultraviolet B (UV-B) radiation induces the extreme production of either reactive oxygen species (ROS) or inflammatory mediators. The aim of this study was to evaluate the antioxidant activities of 70% ethanolic extract of Lablab purpureus (LPE) and the underlying mechanisms using HaCaT cells exposed to UV-B. High-performance liquid chromatography (HPLC) confirmed the presence of gallic acid, catechin, and epicatechin in LPE. LPE was shown to have a very potent capacity to scavenge free radicals. The results showed that LPE prevented DNA damage and inhibited the generation of ROS in HaCaT cells without causing any toxicity. LPE increased the expression of endogenous antioxidant enzymes such as superoxide dismutase-1 and catalase. Furthermore, LPE treatment facilitates the nuclear translocation of nuclear factor (erythroid-derived 2)-like 2 (Nrf-2), boosting the phase II detoxifying enzyme heme oxygenase-1 (HO-1) leading to the combatting of oxidative stress. However, pretreatment of LPE also caused the phosphorylation of mitogen-activated protein kinases (MAPK kinase) (p38 kinase) and extracellular signal-regulated kinase (ERK), whereas treatment with p38 and ERK inhibitors substantially suppressed LPE-induced Nrf2 and heme oxygenase (HO)-1 expression. These findings suggest that LPE exhibits antioxidant activity via Nrf-2-mediated HO-1 signaling through the activation of p38 and ERK, indicating that LPE can potentially be used as a remedy to combat oxidative stress-induced disorder. | Diniyah, Nurud; Alam, Md Badrul; Choi, Hee-Jeong; Lee, Sang-Han | Kyungpook Natl Univ, Grad Sch, Dept Food Sci & Biotechnol, Daegu 41566, South Korea; Univ Jember, Fac Agr Technol, Jember 68121, East Java, Indonesia; Kyungpook Natl Univ, Food & Bioind Res Inst, Daegu 41566, South Korea | ; Lee, Seung Eun/ABG-1607-2021; ALAM, MD BADRUL/AFL-7668-2022; ftp, nurud/AAU-1190-2020; Alam, Md Badrul/AFL-7668-2022 | 57201987396; 56706777100; 57201125608; 57221453703 | nurud.ftp@unej.ac.id;mbalam@knu.ac.kr;choi930302@gmail.com;sang@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 21 | 22 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 5.924 | 22.5 | 0.8 | 2025-06-25 | 11 | 15 | antioxidant; Lablab purpureus; heme oxygenase 1; keratinocyte | ANTIOXIDANT CAPACITY; REACTIVE OXYGEN; IRRADIATION; EXTRACTS; LEGUMES; DAMAGE | Antioxidant; Heme oxygenase 1; Keratinocyte; Lablab purpureus; LPE in aandconcentration-dependentthe Western blot techniquemanner; Ofconcentration-dependentHO-1 were increased bymanner; WasandusedthetoWesternconfirmblotthetechniqueenhanced | Cell Line; Fabaceae; Free Radical Scavengers; Heme Oxygenase-1; Humans; Keratinocytes; MAP Kinase Signaling System; Mitogen-Activated Protein Kinase 1; Mitogen-Activated Protein Kinase 3; NF-E2-Related Factor 2; Oxidative Stress; p38 Mitogen-Activated Protein Kinases; Plant Extracts; Ultraviolet Rays; antioxidant; catalase; catechin; copper zinc superoxide dismutase; epicatechin; gallic acid; heme oxygenase 1; Lablab purpureus extract; messenger RNA; mitogen activated protein kinase 1; mitogen activated protein kinase 3; mitogen activated protein kinase p38; plant extract; stress activated protein kinase; transcription factor Nrf2; unclassified drug; heme oxygenase 1; HMOX1 protein, human; MAPK1 protein, human; MAPK3 protein, human; mitogen activated protein kinase 1; mitogen activated protein kinase 3; mitogen activated protein kinase p38; NFE2L2 protein, human; plant extract; scavenger; transcription factor Nrf2; antioxidant activity; antioxidant responsive element; Article; cell death; controlled study; DNA damage; DPPH radical scavenging assay; enzyme activation; enzyme phosphorylation; ferric reducing antioxidant power; HaCat cell line; high performance liquid chromatography; human; human cell; in vitro study; Lablab purpureus; MAPK signaling; mRNA expression level; oxidative stress; oxygen radical absorbance capacity; protein expression; radioimmunoprecipitation; reverse transcription polymerase chain reaction; ultraviolet B radiation; upregulation; adverse event; biosynthesis; cell line; chemistry; drug effect; Fabaceae; keratinocyte; MAPK signaling; metabolism; radiation response; ultraviolet radiation | English | 2020 | 2020-11 | 10.3390/ijms21228583 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Myricitrin Ameliorates Hyperglycemia, Glucose Intolerance, Hepatic Steatosis, and Inflammation in High-Fat Diet/Streptozotocin-Induced Diabetic Mice | To test the hypothesis that myricitrin (MYR) improves type 2 diabetes, we examined the effect of MYR on hyperglycemia, glucose intolerance, hepatic steatosis, and inflammation in high-fat diet (HFD) and streptozotocin (STZ)-induced type 2 diabetic mice. Male C57BL/6J mice were randomly divided into three groups: non-diabetic, diabetic control, and MYR (0.005%, w/w)-supplemented diabetic groups. Diabetes was induced by HFD and STZ, and MYR was administered orally for 5 weeks. Myricitrin exerted no significant effects on food intake, body weight, fat weight, or plasma lipids levels. However, MYR significantly decreased fasting blood glucose levels, improved glucose intolerance, and increased pancreatic beta-cell mass compared to the diabetic control group. Myricitrin administration also markedly increased glucokinase mRNA expression and activity as well as lowered glucose-6-phosphatase and phosphoenolpyruvate carboxykinase mRNA expression and activity in the liver. In addition, liver weight, hepatic triglyceride content, and lipid droplet accumulation were markedly decreased following MYR administration. These changes were seemingly attributable to the suppression of the hepatic lipogenic enzymes-fatty acid synthase and phosphatidate phosphohydrolase. Myricitrin also significantly lowered plasma MCP-1 and TNF-alpha levels and the mRNA expression of hepatic pro-inflammatory genes. These results suggest that MYR has anti-diabetic potential. | Kim, Do Yeon; Kim, Sang Ryong; Jung, Un Ju | Pukyong Natl Univ, Dept Food Sci & Nutr, 45 Yongso Ro, Busan 48513, South Korea; Kyungpook Natl Univ, Plus KNU Creat BioRes Grp BK21, Sch Life Sci, 1370 San Kyuk, Daegu 41566, South Korea | 59109882800; 56486163800; 7007119425 | dy.kim@kist.re.kr;srk75@knu.ac.kr;jungunju@naver.com; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 21 | 5 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 5.924 | 22.5 | 1.81 | 2025-06-25 | 36 | 39 | myricitrin; diabetes; hyperglycemia; glucose intolerance; hepatic steatosis; inflammation | LIVER-DISEASE; TYPE-2; METABOLISM; MYRICETIN; MODEL; OVEREXPRESSION; GLUCOKINASE; MECHANISMS; DIET | Diabetes; Glucose intolerance; Hepatic steatosis; Hyperglycemia; Inflammation; Myricitrin | Animals; Blood Glucose; Diabetes Mellitus, Experimental; Diabetes Mellitus, Type 2; Diet, High-Fat; Fatty Liver; Flavonoids; Glucokinase; Glucose Intolerance; Hyperglycemia; Inflammation; Insulin Resistance; Lipid Metabolism; Liver; Male; Mice; Mice, Inbred C57BL; Streptozocin; Triglycerides; antidiabetic agent; fat droplet; fatty acid synthase; flavonoid glycoside; glucokinase; glucose; glucose 6 phosphatase; lipid; messenger RNA; monocyte chemotactic protein 1; myricitrin; phosphatidate phosphatase; phosphoenolpyruvate carboxykinase (GTP); streptozocin; triacylglycerol; tumor necrosis factor; unclassified drug; flavonoid; glucokinase; myricitrin; streptozocin; triacylglycerol; animal experiment; animal model; animal tissue; antidiabetic activity; Article; body weight; C57BL 6 mouse; controlled study; drug mechanism; enzyme repression; fatty liver; food intake; glucose blood level; glucose intolerance; hyperglycemia; inflammation; lipid blood level; lipid diet; lipid metabolism; liver weight; male; mouse; mRNA expression level; non insulin dependent diabetes mellitus; nonhuman; pancreas islet beta cell; streptozotocin-induced diabetes mellitus; adverse event; animal; C57BL mouse; drug effect; experimental diabetes mellitus; fatty liver; glucose intolerance; hyperglycemia; inflammation; insulin resistance; liver; metabolism; non insulin dependent diabetes mellitus | English | 2020 | 2020-03 | 10.3390/ijms21051870 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | NOX1 Inhibition Attenuates Kidney Ischemia-Reperfusion Injury via Inhibition of ROS-Mediated ERK Signaling | The protective effects of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) 1 inhibition against kidney ischemia-reperfusion injury (IRI) remain uncertain. The bilateral kidney pedicles of C57BL/6 mice were clamped for 30 min to induce IRI. Madin-Darby Canine Kidney (MDCK) cells were incubated with H2O2(1.4 mM) for 1 h to induce oxidative stress. ML171, a selective NOX1 inhibitor, and siRNA against NOX1 were treated to inhibit NOX1. NOX expression, oxidative stress, apoptosis assay, and mitogen-activated protein kinase (MAPK) pathway were evaluated. The kidney function deteriorated and the production of reactive oxygen species (ROS), including intracellular H(2)O(2)production, increased due to IRI, whereas IRI-mediated kidney dysfunction and ROS generation were significantly attenuated by ML171. H(2)O(2)evoked the changes in oxidative stress enzymes such as SOD2 and GPX in MDCK cells, which was mitigated by ML171. Treatment with ML171 and transfection with siRNA against NOX1 decreased the upregulation of NOX1 and NOX4 induced by H(2)O(2)in MDCK cells. ML171 decreased caspase-3 activity, the Bcl-2/Bax ratio, and TUNEL-positive tubule cells in IRI mice and H2O2-treated MDCK cells. Among the MAPK pathways, ML171 affected ERK signaling by ERK phosphorylation in kidney tissues and tubular cells. NOX1-selective inhibition attenuated kidney IRI via inhibition of ROS-mediated ERK signaling. | Jung, Hee-Yeon; Oh, Se-Hyun; Ahn, Ji-Sun; Oh, Eun-Joo; Kim, You-Jin; Kim, Chan-Duck; Park, Sun-Hee; Kim, Yong-Lim; Cho, Jang-Hee | Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Div Nephrol,Dept Internal Med, Daegu 41944, South Korea | ; Kim, Yong-Lim/AGK-3172-2022; Jung, Hee-Yeon/AFB-8578-2022; Cho, Jang-hee/ABD-3534-2020; Park, Sun-Hee/LMN-0033-2024 | 57196396467; 56053033900; 57191631694; 35783223700; 57190286137; 8558530700; 7501831741; 55633533600; 7403536291 | hy-jung@knu.ac.kr;ttily@nate.com;ggumsuni@hanmail.net;oej1124@naver.com;pinkqic1004@naver.com;drcdkim@knu.ac.kr;sh-park@knu.ac.kr;ylkim@knu.ac.kr;jh-cho@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 21 | 18 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 5.924 | 22.5 | 1.3 | 2025-06-25 | 27 | 30 | NOX1; ML171; reactive oxygen species; ERK; ischemia-reperfusion injury; acute kidney injury | RENAL ISCHEMIA/REPERFUSION INJURY; OXIDASE COMPONENT P47(PHOX); INDUCED OXIDATIVE STRESS; PROTEIN-KINASE-C; NADPH OXIDASE; CELL BIOLOGY; NITRIC-OXIDE; ACTIVATION; ANTIOXIDANT; EXPRESSION | Acute kidney injury; ERK; Ischemia-reperfusion injury; ML171; NOX1; Reactive oxygen species | Animals; Dogs; Hydrogen Peroxide; Kidney; Kidney Diseases; Madin Darby Canine Kidney Cells; Male; MAP Kinase Signaling System; Mice; NADPH Oxidase 1; Reperfusion Injury; 2 acetylphenothiazine; caspase 3; creatinine; hydrogen peroxide; mitogen activated protein kinase; ml 171; oxidoreductase inhibitor; protein Bax; protein bcl 2; reactive oxygen metabolite; reduced nicotinamide adenine dinucleotide phosphate oxidase 1; reduced nicotinamide adenine dinucleotide phosphate oxidase 4; small interfering RNA; unclassified drug; hydrogen peroxide; NOX1 protein, mouse; reduced nicotinamide adenine dinucleotide phosphate oxidase 1; animal cell; animal experiment; animal model; animal tissue; apoptosis assay; Article; controlled study; creatinine blood level; drug selectivity; enzyme activity; enzyme inhibition; genetic transfection; histopathology; kidney function; kidney tissue; kidney tubule cell; male; MAPK signaling; MDCK cell line; mouse; nonhuman; oxidative stress; protein expression; protein phosphorylation; renal ischemia reperfusion injury; TUNEL assay; upregulation; urea nitrogen blood level; animal; dog; enzymology; kidney; kidney disease; MAPK signaling; metabolism; pathology; reperfusion injury | English | 2020 | 2020-09 | 10.3390/ijms21186911 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Orphan Nuclear Receptor ERRγ Is a Novel Transcriptional Regulator of IL-6 Mediated Hepatic BMP6 Gene Expression in Mice | Bone morphogenetic protein 6 (BMP6) is a multifunctional growth factor involved in organ development and homeostasis. BMP6 controls expression of the liver hormone, hepcidin, and thereby plays a crucial role in regulating iron homeostasis. BMP6 gene transcriptional regulation in liver is largely unknown, but would be of great help to externally modulate iron load in pathologic conditions. Here, we describe a detailed molecular mechanism of hepatic BMP6 gene expression by an orphan nuclear receptor, estrogen-related receptor gamma (ERR gamma), in response to the pro-inflammatory cytokine interleukin 6 (IL-6). Recombinant IL-6 treatment increases hepatic ERR gamma and BMP6 expression. Overexpression of ERR gamma is sufficient to increase BMP6 gene expression in hepatocytes, suggesting that IL-6 is upstream of ERR gamma. In line, knock-down of ERR gamma in cell lines or a hepatocyte specific knock-out of ERR gamma in mice significantly decreases IL-6 mediated BMP6 expression. Promoter studies show that ERR gamma directly binds to the ERR response element (ERRE) in the mouse BMP6 gene promoter and positively regulates BMP6 gene transcription in IL-6 treatment conditions, which is further confirmed by ERRE mutated mBMP6-luciferase reporter assays. Finally, an inverse agonist of ERR gamma, GSK5182, markedly inhibits IL-6 induced hepatic BMP6 expression in vitro and in vivo. Taken together, these results reveal a novel molecular mechanism on ERR gamma mediated transcriptional regulation of hepatic BMP6 gene expression in response to IL-6. | Radhakrishnan, Kamalakannan; Kim, Yong-Hoon; Jung, Yoon Seok; Kim, Jina; Kim, Don-Kyu; Cho, Sung Jin; Lee, In-Kyu; Dooley, Steven; Lee, Chul-Ho; Choi, Hueng-Sik | Chonnam Natl Univ, Sch Biol Sci & Technol, Gwangju 61186, South Korea; Korea Res Inst Biosci & Biotechnol, Lab Anim Resource Ctr, Daejeon 34141, South Korea; Univ Sci & Technol UST, KRIBB Sch Biosci, Dept Funct Genom, Daejeon 34141, South Korea; Daegu Gyeongbuk Med Innovat Fdn, New Drug Dev Ctr, Daegu 41061, South Korea; Chonnam Natl Univ, Dept Integrat Food Biosci & Biotechnol, Gwangju 61186, South Korea; Kyungpook Natl Univ Hosp, Leading Edge Res Ctr Drug Discovery & Dev Diabet, Daegu 41404, South Korea; Kyungpook Natl Univ, Dept Internal Med, Sch Med, Kyungpook Natl Univ Hosp, Daegu 41944, South Korea; Heidelberg Univ, Dept Med 2, Med Fac Mannheim, D-68167 Mannheim, Germany | Jung, Yoon/B-8512-2011; Lee, Chul-Ho/MBV-8603-2025; Radhakrishnan, Kamalakannan/HNP-0477-2023; Lee, In-Kyu/AAR-6374-2021; Dooley, Steven/T-6491-2018 | 57217673988; 57210989406; 57203348590; 56949261900; 37081358700; 58735369700; 36071537600; 35402441200; 56223516500; 7404338771 | kmknphd@gmail.com;yhoonkim@kribb.re.kr;yhemm@naver.com;jina@dgmif.re.kr;dkkim2@jnu.ac.kr;sjcho@dgmif.re.kr;leei@knu.ac.kr;Steven.Dooley@medma.uni-heidelberg.de;chullee@kribb.re.kr;hsc@chonnam.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 21 | 19 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 5.924 | 22.5 | 0.65 | 2025-06-25 | 14 | 16 | estrogen-related receptor γ (ERRγ ); bone morphogenetic protein 6 (BMP6); interleukin 6 (IL-6); orphan receptors; transcription; gene expression and regulation; liver | HEPCIDIN EXPRESSION; INVERSE AGONIST; BONE-FORMATION; LIVER; HOMEOSTASIS; BETA; IDENTIFICATION; SUPERFAMILY; INHIBITION; FIBROSIS | Bone morphogenetic protein 6 (BMP6); Estrogen-related receptor γ (ERRγ); Gene expression and regulation; Interleukin 6 (IL-6); Liver; Orphan receptors; Transcription | Animals; Binding Sites; Bone Morphogenetic Protein 6; Genes, Reporter; Hep G2 Cells; Hepatocytes; Hepcidins; Humans; Interleukin-6; Iron; Liver; Luciferases; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Promoter Regions, Genetic; Protein Binding; Receptors, Estrogen; Response Elements; RNA, Small Interfering; Signal Transduction; Tamoxifen; Transcriptional Activation; bone morphogenetic protein 6; estrogen related receptor gamma; interleukin 6; messenger RNA; orphan nuclear receptor; unclassified drug; Bmp6 protein, mouse; bone morphogenetic protein 6; Esrrg protein, mouse; estrogen receptor; GSK5182; hepcidin; interleukin 6; interleukin-6, mouse; iron; luciferase; protein binding; small interfering RNA; tamoxifen; animal cell; animal experiment; animal model; Article; controlled study; gene expression; genetic transcription; immunohistochemistry; liver cell; mouse; nonhuman; promoter region; protein expression; real time polymerase chain reaction; regulatory mechanism; reverse transcription; RNA extraction; transcription regulation; upregulation; animal; binding site; C57BL mouse; cytology; DNA responsive element; drug effect; genetics; Hep-G2 cell line; human; knockout mouse; liver; male; metabolism; reporter gene; signal transduction; transcription initiation | English | 2020 | 2020-10 | 10.3390/ijms21197148 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Physiological Importance of Pectin Modifying Genes During Rice Pollen Development | Although cell wall dynamics, particularly modification of homogalacturonan (HGA, a major component of pectin) during pollen tube growth, have been extensively studied in dicot plants, little is known about how modification of the pollen tube cell wall regulates growth in monocot plants. In this study, we assessed the role of HGA modification during elongation of the rice pollen tube by adding a pectin methylesterase (PME) enzyme or a PME-inhibiting catechin extract (Polyphenon 60) to in vitro germination medium. Both treatments led to a severe decrease in the pollen germination rate and elongation. Furthermore, using monoclonal antibodies toward methyl-esterified and de-esterified HGA epitopes, it was found that exogenous treatment of PME and Polyphenon 60 resulted in the disruption of the distribution patterns of low- and high-methylesterified pectins upon pollen germination and during pollen tube elongation. Eleven PMEs and 13 PME inhibitors (PMEIs) were identified by publicly available transcriptome datasets and their specific expression was validated by qRT-PCR. Enzyme activity assays and subcellular localization using a heterologous expression system in tobacco leaves demonstrated that some of the pollen-specific PMEs and PMEIs possessed distinct enzymatic activities and targeted either the cell wall or other compartments. Taken together, our findings are the first line of evidence showing the essentiality of HGA methyl-esterification status during the germination and elongation of pollen tubes in rice, which is primarily governed by the fine-tuning of PME and PMEI activities. | Kim, Yu-Jin; Jeong, Ho Young; Kang, Seung-Yeon; Silva, Jeniffer; Kim, Eui-Jung; Park, Soon Ki; Jung, Ki-Hong; Lee, Chanhui | Kyung Hee Univ, Grad Sch Biotechnol, Yongin 17104, South Korea; Kyung Hee Univ, Crop Biotech Inst, Yongin 17104, South Korea; Kyung Hee Univ, Coll Life Sci, Dept Plant & Environm New Resources, Yongin 17104, South Korea; Kyungpook Natl Univ, Sch Appl Biosci, Daegu 41566, South Korea | ; Kim, YuJin/NQE-8241-2025; Jung, Ki/L-5570-2019 | 57074286200; 57207963667; 57217865985; 56780864200; 57214231436; 8055974900; 56022522000; 23005580300 | yujinkim@khu.ac.kr;ratank@khu.ac.kr;moss7894@naver.com;jeniffersilva.yat@gmail.com;alice804@khu.ac.kr;psk@knu.ac.kr;khjung2010@khu.ac.kr;chan521@khu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 21 | 14 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 5.924 | 22.5 | 0.94 | 2025-06-25 | 22 | 20 | rice; pollen; pectin; pectin methylesterase; pectin methylesterase inhibitor; pollen tube growth | CELL-WALL; TUBE TIP; METHYLESTERASE; ARABIDOPSIS; GROWTH; IDENTIFICATION; INHIBITION; EXPRESSION; SEPARATION; REGULATOR | Pectin; Pectin methylesterase; Pectin methylesterase inhibitor; Pollen; Pollen tube growth; Rice | Carboxylic Ester Hydrolases; Cell Wall; Gene Expression Regulation, Plant; Germination; Oryza; Pectins; Plant Leaves; Plant Proteins; Pollen Tube; Polyphenols; Tobacco; Transcriptome; alcohol oxidase; beta glucuronidase; boric acid; bovine serum albumin; buffer; catechin; epitope; formaldehyde dehydrogenase; galacturonic acid; homogalacturonan; monoclonal antibody; paraformaldehyde; pectin; pectinesterase; phosphate buffer; polyphenon 60; thiamine; transcriptome; unclassified drug; carboxylesterase; pectin; pectinesterase; plant protein; polygalacturonic acid; polyphenol; polyphenon-60; transcriptome; amino acid sequence; antibody labeling; Article; cellular distribution; confocal laser scanning microscopy; controlled study; enzyme activity; esterification; gene expression; gene overexpression; genome-wide association study; immunofluorescence; immunolocalization; microscopy; modifier gene; Nicotiana benthamiana; nonhuman; phylogenetic tree; phylogenomics; plant growth; plant physiology; plasmolysis; pollen development; pollen germination; pollen tube growth; protein modification; real time polymerase chain reaction; rice; RNA sequence; cell wall; drug effect; gene expression regulation; genetics; germination; Oryza; plant leaf; pollen tube; tobacco | English | 2020 | 2020-07 | 10.3390/ijms21144840 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Protective Effect of Tetrahydroquinolines from the Edible Insect Allomyrina dichotoma on LPS-Induced Vascular Inflammatory Responses | The larva of Allomyrina dichotoma (family Scarabaeidae) is an edible insect that is registered in the Korean Food Standards Codex as a food resource. The chemical study on the larvae of A. dichotoma resulted in the isolation of three new tetrahydroquinolines, allomyrinaines A-C (1-3), one new dopamine derivative, allomyrinamide A (4), and four known compounds (5-8). The structures were elucidated on the basis of 1D and 2D nuclear magnetic resonance (NMR) and MS spectroscopic data analysis. Allomyrinaines A-C (1-3) possessed three stereogenic centers at C-2, C-3, and C-4, whose relative configurations were determined by analyses of the coupling constants and the nuclear Overhauser enhancement spectroscopy (NOESY) data, as well as DP4+ calculation. The anti-inflammatory effects of compounds 1-4 were evaluated in human endothelial cells. Allomyrinaines A-C (1-3) could stabilize vascular barrier integrity on lipopolysaccharide (LPS)-induced vascular inflammation via inhibition of the nuclear factor-kappa B (NF-kappa B) pathway. The physiologically relevant concentration was confirmed by Q-TOF-MS-based quantitative analysis on allomyrinaines A-C in crude extract. This study suggests that allomyrinaines A-C (1-3) are bioactive constituents of A. dichotoma to treat vascular inflammatory disorder. | Park, InWha; Lee, Wonhwa; Yoo, Youngbum; Shin, Hyosoo; Oh, Joonseok; Kim, Hyelim; Kim, Mi-Ae; Hwang, Jae Sam; Bae, Jong-Sup; Na, MinKyun | Chungnam Natl Univ, Coll Pharm, Daejeon 34134, South Korea; Korea Res Inst Biosci & Biotechnol KRIBB, Aging Res Ctr, Daejeon 34141, South Korea; Yale Univ, Dept Chem, New Haven, CT 06520 USA; Natl Acad Agr Sci, RDA, Dept Agr Biol, Suwon 55365, South Korea; Kyungpook Natl Univ, BK21 Plus KNU Multiom Based Creat Drug Res Team, Pharmaceut Sci Res Inst, CMRI,Coll Pharm, Daegu 41566, South Korea | ; Kim, Juhee/KFS-3069-2024; Bae, Jong-Sup/AAU-9724-2020; na, ma/K-4873-2013; Lee, Wonhwa/GLQ-6506-2022 | 57195484915; 50161632800; 57204017274; 57218711481; 7402155599; 57210146478; 56911258300; 16637012100; 16021543200; 7006636995 | inwha129@naver.com;wonhwalee@kribb.re.kr;ddq3416@gmail.com;hysooo0914@gmail.com;joonseok.oh@yale.edu;rimeeyo@gmail.com;kimma@korea.kr;hwangjs@korea.kr;baejs@knu.ac.kr;mkna@cnu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 21 | 10 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 5.924 | 22.5 | 0.8 | 2025-06-25 | 15 | 16 | Allomyrina dichotoma; anti-inflammatory effect; dopamine derivative; NF-kappa B; tetrahydroquinoline | LUNG INJURY; MECHANISMS | Allomyrina dichotoma; Anti-inflammatory effect; Dopamine derivative; NF-κB; Tetrahydroquinoline | Animals; Anti-Inflammatory Agents; Coleoptera; Cytokines; Edible Insects; Endothelial Cells; Humans; Inflammation; Lipopolysaccharides; Magnetic Resonance Spectroscopy; Male; Mice, Inbred C57BL; Molecular Structure; NF-kappa B; Protective Agents; Quinolines; 1,2,3,4 tetrahydroquinoline derivative; alanine aminotransferase; arbutin; aspartate aminotransferase; creatinine; dopamine; essential oil; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; intercellular adhesion molecule 1; lactate dehydrogenase; lipopolysaccharide; tumor necrosis factor; vascular cell adhesion molecule 1; 1,2,3,4-tetrahydroquinoline; antiinflammatory agent; cytokine; immunoglobulin enhancer binding protein; lipopolysaccharide; protective agent; quinoline derivative; Allomyrina dichotoma; antiinflammatory activity; Article; beetle; binding site; carbon nuclear magnetic resonance; cell viability; computer assisted tomography; controlled study; cytotoxicity; electrospray mass spectrometry; endothelial dysfunction; enzyme linked immunosorbent assay; heteronuclear single quantum coherence; high performance liquid chromatography; IC50; infrared spectroscopy; leukocyte count; liquid chromatography-mass spectrometry; liver injury; mass fragmentography; molecular docking; multiple sclerosis; nonhuman; protein phosphorylation; proton nuclear magnetic resonance; quantitative analysis; septic shock; signal transduction; structure activity relation; thin layer chromatography; time of flight mass spectrometry; upregulation; urea nitrogen blood level; vasculitis; X ray crystallography; animal; beetle; C57BL mouse; chemical structure; chemistry; drug effect; endothelium cell; human; inflammation; male; metabolism; nuclear magnetic resonance spectroscopy | English | 2020 | 2020-05 | 10.3390/ijms21103406 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Renoprotective Effects of Alpha-1 Antitrypsin against Tacrolimus-Induced Renal Injury | The protective effects of alpha-1 antitrypsin (AAT) in tacrolimus (TAC)-induced renal injury was evaluated in a rat model. The TAC group rats were subcutaneously injected with 2 mg/kg TAC every day for four weeks. The TAC with AAT group was cotreated with daily subcutaneous injections of TAC and intraperitoneal injections of AAT (80 mg/kg) for four weeks. The effects of AAT on TAC-induced renal injury were evaluated using serum biochemistry, histopathology, and Western blotting. The TAC injection significantly increased renal interstitial fibrosis, inflammation, and apoptosis as compared to the control treatment. The histopathological examination showed that cotreatment of TAC and AAT attenuated interstitial fibrosis (collagen, fibronectin, and alpha-SMA staining), and alpha-SMA expression in Western blotting was also decreased. Immunohistochemical staining for inflammation (osteopontin and ED-1 staining) revealed improved interstitial inflammation in the TAC with AAT group compared to that in the TAC group. The TAC treatment increased renal apoptosis compared to the control treatment, based on the results of increased immunohistochemical staining of terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL), increased caspase-3 activity, and lower Bcl-2 to Bad expression ratio. However, AAT cotreatment significantly changed these markers and consequently showed decreased apoptosis. AAT protects against TAC-induced renal injury via antifibrotic, anti-inflammatory, and antiapoptotic effects. | Lim, Jeong-Hoon; Oh, Eun-Joo; Oh, Se-Hyun; Jung, Hee-Yeon; Choi, Ji-Young; Cho, Jang-Hee; Park, Sun-Hee; Kim, Yong-Lim; Kim, Chan-Duck | Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Div Nephrol,Dept Internal Med, Daegu 41944, South Korea | Kim, Yong-Lim/AGK-3172-2022; Cho, Jang-hee/ABD-3534-2020; Lim, Jeong-Hoon/ABE-6003-2020; Park, Sun-Hee/LMN-0033-2024 | 55360244300; 35783223700; 56053033900; 57196396467; 7501393222; 7403536291; 7501831741; 55633533600; 8558530700 | jh-lim@knu.ac.kr;oej1124@naver.com;ttily@nate.com;hy-jung@knu.ac.kr;jyss1002@hanmail.net;jh-cho@knu.ac.kr;sh-park@knu.ac.kr;ylkim@knu.ac.kr;drcdkim@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 21 | 22 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 5.924 | 22.5 | 0.14 | 2025-06-25 | 5 | 4 | alpha-1 antitrypsin; tacrolimus-induced renal injury; fibrosis; inflammation; apoptosis | INDUCED NEPHROTOXICITY; OSTEOPONTIN; INHIBITION; PROTECTS; FIBROSIS; TRANSPLANTATION; EXPRESSION; CASPASE-3; CELLS | Alpha-1 antitrypsin; Apoptosis; Fibrosis; Inflammation; Tacrolimus-induced renal injury | Acute Kidney Injury; alpha 1-Antitrypsin; Animals; Anti-Inflammatory Agents; Apoptosis; Fibrosis; Gene Expression Regulation; Male; Rats; Rats, Sprague-Dawley; Tacrolimus; alpha 1 antitrypsin concentrate; alpha smooth muscle actin; biological marker; caspase 3; collagen; creatinine; fibronectin; osteopontin; protein; protein BAD; protein bcl 2; protein ED 1; tacrolimus; unclassified drug; alpha 1 antitrypsin; antiinflammatory agent; tacrolimus; animal experiment; animal model; animal tissue; antiapoptotic activity; antifibrotic activity; antiinflammatory activity; apoptosis; Article; controlled study; enzyme activity; histopathology; immunohistochemistry; inflammation; kidney fibrosis; kidney injury; male; nephrotoxicity; nonhuman; protein expression; protein function; rat; renal protection; TUNEL assay; urea nitrogen blood level; Western blotting; acute kidney failure; animal; drug effect; fibrosis; gene expression regulation; metabolism; pathology; Sprague Dawley rat | English | 2020 | 2020-11 | 10.3390/ijms21228628 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | rhBMP-2 Pre-Treated Human Periodontal Ligament Stem Cell Sheets Regenerate a Mineralized Layer Mimicking Dental Cementum | The periodontal complex consisting of alveolar bone, cementum, and periodontal ligaments (PDL) supports human teeth through the systematic orchestration of mineralized tissues and fibrous tissues. Importantly, cementum, the outermost mineralized layer of dental roots, plays an essential role by bridging the inner ligaments from the dental root to the alveolar bone. When the periodontal complex is damaged, the regeneration of each component of the periodontal complex is necessary; however, it is still challenging to achieve complete functional regeneration. In this study, we tried to control the regeneration of cementum and PDL by using a human PDL stem cell (hPDLSC) sheet engineering technology with the pretreatment of recombinant human BMP-2 (rhBMP-2). Isolated hPDLSCs obtained from extracted human teeth were pretreated with rhBMP-2 for in vitro osteogenic differentiation and grafted on the micro/macro-porous biphasic calcium phosphate (MBCP) blocks, which represent dental roots. The MBCPs with hPDLSC sheets were implanted in the subcutaneous layer of immune-compromised mice, and rhBMP-2 pretreated hPDLSC sheets showed higher mineralization and collagen ligament deposition than the no-pretreatment group. Therefore, the rhBMP-2-hPDLSC sheet technique could be an effective strategy for the synchronized regeneration of two different tissues: mineralized tissue and fibrous tissues in periodontal complexes. | Park, Joo-Young; Park, Chan Ho; Yi, TacGhee; Kim, Si-na; Iwata, Takanori; Yun, Jeong-Ho | Seoul Natl Univ, Dept Oral & Maxillofacial Surg, Dent Hosp, Seoul 03080, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Dent Biomat, Daegu 41940, South Korea; Kyungpook Natl Univ, Inst Biomat Res & Dev, Daegu 41940, South Korea; SunCreate Co Ltd, Uijongbu 11813, Gyeonggi Do, South Korea; Inha Univ, Dept Biomed Sci, Sch Med, Drug Dev Program, Incheon 22332, South Korea; SCM Lifesci Co Ltd, Incheon 22332, South Korea; Tokyo Med & Dent Univ, Grad Sch Med & Dent Sci, Dept Periodontol, Tokyo 1138549, Japan; Jeonbuk Natl Univ, Coll Dent & Inst Oral Biosci, Dept Periodontol, Jeonju 54896, South Korea; Jeonbuk Natl Univ, Biomed Res Inst, Jeonbuk Natl Univ Hosp, Res Inst Clin Med, Jeonju 54907, South Korea | ; Iwata, Takanori/AAT-3219-2020 | 36448526700; 55728043300; 14033581500; 56169063700; 7203071784; 55154133000 | bbyoung1@snu.ac.kr;chanho@knu.ac.kr;tgwise@naver.com;tlsk1206@naver.com;iwata.peri@tmd.ac.jp;grayheron@hanmail.net; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 21 | 11 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 5.924 | 22.5 | 1.3 | 2025-06-25 | 22 | 25 | dental stem cells; stem cell therapy; mesenchymal stem cell; regeneration; tissue engineering | SCAFFOLD DESIGN; TISSUE; DIFFERENTIATION; EFFICACY; THERAPY | Dental stem cells; Mesenchymal stem cell; Regeneration; Stem cell therapy; Tissue engineering | Animals; Bone Morphogenetic Protein 2; Cells, Cultured; Dental Cementum; Humans; Hydroxyapatites; Male; Mesenchymal Stem Cells; Mice; Mice, Inbred BALB C; Periodontal Ligament; Recombinant Proteins; Regeneration; Stem Cell Transplantation; Tissue Engineering; Tissue Scaffolds; calcium phosphate; recombinant bone morphogenetic protein 2; bone morphogenetic protein 2; hydroxyapatite; hydroxyapatite-beta tricalcium phosphate; recombinant protein; adult; animal experiment; animal tissue; Article; bone development; cell differentiation; cell isolation; controlled study; dental pulp stem cell; human; human cell; in vitro study; male; mouse; nonhuman; periodontal ligament; premolar tooth; tissue engineering; tissue regeneration; tooth cementum; tooth development; tooth root; animal; Bagg albino mouse; cell culture; chemistry; cytology; drug effect; mesenchymal stem cell; metabolism; periodontal ligament; physiology; procedures; regeneration; stem cell transplantation; tissue scaffold; tooth cementum | English | 2020 | 2020-06 | 10.3390/ijms21113767 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Review | Root Response to Drought Stress in Rice (Oryza sativa L.) | The current unpredictable climate changes are causing frequent and severe droughts. Such circumstances emphasize the need to understand the response of plants to drought stress, especially in rice, one of the most important grain crops. Knowledge of the drought stress response components is especially important in plant roots, the major organ for the absorption of water and nutrients from the soil. Thus, this article reviews the root response to drought stress in rice. It is presented to provide readers with information of use for their own research and breeding program for tolerance to drought stress in rice. | Kim, Yoonha; Chung, Yong Suk; Lee, Eungyeong; Tripathi, Pooja; Heo, Seong; Kim, Kyung-Hwan | Kyungpook Natl Univ, Sch Appl Biosci, Daegu 41566, South Korea; Jeju Natl Univ, SARI, Coll Appl Life Sci, Fac Biosci & Ind, Jeju 63243, South Korea; RDA, Natl Inst Agr Sci, Jeonju 54874, South Korea; Ganghwa Agr Technol Serv Ctr, Incheon 23038, South Korea | Chung, Yong/V-6909-2019; Lee, Chaewon/HPD-8056-2023; Kim, Kyung/J-5382-2012 | 57224866763; 36983850100; 57209278805; 57215084745; 35898345700; 57202965738 | kyh1229@knu.ac.kr;yschung@jejunu.ac.kr;wowlek44@korea.kr;pooja@knu.ac.kr;sycarus@korea.kr;biopiakim@korea.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 21 | 4 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 5.924 | 22.5 | 4.14 | 2025-06-25 | 213 | 243 | root morphological trait; root architecture; physiological response to drought; screening methods for drought stress; phenomics | QUANTITATIVE TRAIT LOCI; PLANT-SOIL FEEDBACKS; WATER-UPTAKE; ABIOTIC STRESS; SYSTEM ARCHITECTURE; GRAIN-YIELD; STOMATAL CONDUCTANCE; MORPHOLOGICAL TRAITS; RESOURCE LIMITATION; COMMUNITY RESPONSES | Phenomics; Physiological response to drought; Root architecture; Root morphological trait; Screening methods for drought stress | Adaptation, Physiological; Droughts; Gene Expression Regulation, Plant; Oryza; Plant Breeding; Plant Proteins; Plant Roots; Soil; Stress, Physiological; Water; plant protein; water; breeding; climate change; crop; drought stress; morphological trait; nonhuman; nutrient; phenomics; plant root; review; rice; soil; water transport; adaptation; chemistry; drought; gene expression regulation; genetics; metabolism; Oryza; physiological stress; physiology; plant breeding; plant root | English | 2020 | 2020-02 | 10.3390/ijms21041513 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Salinity Stress-Mediated Suppression of Expression of Salt Overly Sensitive Signaling Pathway Genes Suggests Negative Regulation by AtbZIP62 Transcription Factor in Arabidopsis thaliana | Salt stress is one of the most serious threats in plants, reducing crop yield and production. The salt overly sensitive (SOS) pathway in plants is a salt-responsive pathway that acts as a janitor of the cell to sweep out Na+ ions. Transcription factors (TFs) are key regulators of expression and/or repression of genes. The basic leucine zipper (bZIP) TF is a large family of TFs regulating various cellular processes in plants. In the current study, we investigated the role of the Arabidopsis thalianabZIP62 TF in the regulation of SOS signaling pathway by measuring the transcript accumulation of its key genes such as SOS1, 2, and 3, in both wild-type (WT) and atbzip62 knock-out (KO) mutants under salinity stress. We further observed the activation of enzymatic and non-enzymatic antioxidant systems in the wild-type, atbzip62, atcat2 (lacking catalase activity), and atnced3 (lacking 9-cis-epoxycarotenoid dioxygenase involved in the ABA pathway) KO mutants. Our findings revealed that atbzip62 plants exhibited an enhanced salt-sensitive phenotypic response similar to atnced3 and atcat2 compared to WT, 10 days after 150 mM NaCl treatment. Interestingly, the transcriptional levels of SOS1, SOS2, and SOS3 increased significantly over time in the atbzip62 upon NaCl application, while they were downregulated in the wild type. We also measured chlorophyll a and b, pheophytin a and b, total pheophytin, and total carotenoids. We observed that the atbzip62 exhibited an increase in chlorophyll and total carotenoid contents, as well as proline contents, while it exhibited a non-significant increase in catalase activity. Our results suggest that AtbZIP62 negatively regulates the transcriptional events of SOS pathway genes, AtbZIP18 and AtbZIP69 while modulating the antioxidant response to salt tolerance in Arabidopsis. | Rolly, Nkulu Kabange; Imran, Qari Muhammad; Lee, In-Jung; Yun, Byung-Wook | Kyungpook Natl Univ, Sch Appl Biosci, Lab Plant Funct Genom, Daegu 702701, South Korea; Minist Agr, SENASEM, Natl Lab Seed Testing, Natl Seed Serv, 904KIN1, Kinshasa, DEM REP CONGO; Kyungpook Natl Univ, Sch Appl Biosci, Lab Crop Physiol, Daegu 41566, South Korea | KABANGE, NKULU/AAQ-9425-2020; Lee, In-Jung/GLS-0432-2022; Imran, Qari Muhammad/ABG-6074-2022 | 57202031236; 55849263700; 16425830900; 8245123600 | rolly.kabange@gmail.com;mimranbot@gmail.com;ijlee@knu.ac.kr;bwyun@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 21 | 5 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 5.924 | 22.5 | 3.26 | 2025-06-25 | 48 | 53 | AtbZIP62; transcription regulation; SOS signaling pathway; salt tolerance; Arabidopsis | PROTEIN-KINASE SOS2; LIPID-PEROXIDATION; DROUGHT TOLERANCE; S-NITROSYLATION; SOIL-SALINITY; GROWTH; PROLINE; TRANSPORT; EXCHANGE; ENCODES | Arabidopsis; AtbZIP62; Salt tolerance; SOS signaling pathway; Transcription regulation | Arabidopsis; Arabidopsis Proteins; Basic-Leucine Zipper Transcription Factors; Carotenoids; Gene Expression Regulation, Plant; Sodium Chloride; antioxidant; carotenoid; catalase; chlorophyll; chlorophyll a; chlorophyll b; malonaldehyde; pheophytin; SOS protein; transcription factor; Arabidopsis protein; basic leucine zipper transcription factor; sodium chloride; Arabidopsis; Arabidopsis thaliana; Article; cotyledon; enzyme activity; gene expression; genotype; glutamate synthaseencoding gene 1; loss of function mutation; nonhuman; oxidative stress; plant gene; protein expression; real time polymerase chain reaction; salinity; salt stress; salt tolerance; screening; signal transduction; upregulation; drug effect; gene expression regulation; genetics; metabolism | English | 2020 | 2020-03 | 10.3390/ijms21051726 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Screening of an Epigenetic Drug Library Identifies 4-((hydroxyamino)carbonyl)-N-(2-hydroxyethyl)-N-Phenyl-Benzeneacetamide that Reduces Melanin Synthesis by Inhibiting Tyrosinase Activity Independently of Epigenetic Mechanisms | The aim of this study was to identify novel antimelanogenic drugs from an epigenetic screening library containing various modulators targeting DNA methyltransferases, histone deacetylases, and other related enzymes/proteins. Of 141 drugs tested, K8 (4-((hydroxyamino)carbonyl)-N-(2-hydroxyethyl)-N-phenyl-benzeneacetamide; HPOB) was found to effectively inhibit the alpha-melanocyte-stimulating hormone (alpha-MSH)-induced melanin synthesis in B16-F10 murine melanoma cells without accompanying cytotoxicity. Additional experiments showed that K8 did not significantly reduce the mRNA and protein level of tyrosinase (TYR) or microphthalmia-associated transcription factor (MITF) in cells, but it potently inhibited the catalytic activity TYR in vitro (IC50, 1.1-1.5 mu M) as compared to beta-arbutin (IC50, 500-700 mu M) or kojic acid (IC50, 63 mu M). K8 showed copper chelating activity similar to kojic acid. Therefore, these data suggest that K8 inhibits cellular melanin synthesis not by downregulation of TYR protein expression through an epigenetic mechanism, but by direct inhibition of TYR catalytic activity through copper chelation. Metal chelating activity of K8 is not surprising because it is known to inhibit histone deacetylase (HDAC) 6 through zinc chelation. This study identified K8 as a potent inhibitor of cellular melanin synthesis, which may be useful for the treatment of hyperpigmentation disorders | Song, Hyerim; Hwang, Yun Jeong; Ha, Jae Won; Boo, Yong Chool | Kyungpook Natl Univ, Sch Med, Dept Mol Med, Daegu 41944, South Korea; Kyungpook Natl Univ, Brain Korea BK 21 Plus Kyungpook Natl Univ KNU Bi, Daegu 41944, South Korea; Kyungpook Natl Univ, Cell & Matrix Res Inst, Daegu 41944, South Korea | 57210165562; 57217489746; 57210154932; 6602899130 | happylove951@naver.com;hwangkyy26@naver.com;jaewon1226@knu.ac.kr;ycboo@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 21 | 13 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 5.924 | 22.5 | 0.36 | 2025-06-25 | 8 | 7 | melanin; epigenetic screening library; antimelanogenic drug; tyrosinase; pigmentation; 4-((hydroxyamino)carbonyl)-N-(2-hydroxyethyl)-N-phenyl-benzeneacetamide; HPOB; tyrosinase inhibitor | HYDROXAMIC ACID-DERIVATIVES; SKIN WHITENING AGENTS; P-COUMARIC ACID; MELANOGENESIS; PIGMENTATION; EXPRESSION; DEPIGMENTATION; SUPPRESSION; RESVERATROL; CHEMISTRY | 4-((hydroxyamino)carbonyl)-N-(2-hydroxyethyl)-N-phenyl-benzeneacetamide; Antimelanogenic drug; Epigenetic screening library; HPOB; Melanin; Pigmentation; Tyrosinase; Tyrosinase inhibitor | Animals; Antineoplastic Agents; Benzeneacetamides; Epigenesis, Genetic; Melanins; Melanoma, Experimental; Mice; Microphthalmia-Associated Transcription Factor; Monophenol Monooxygenase; Pharmaceutical Preparations; Tumor Cells, Cultured; 4 ((hydroxyamino) carbonyl) n (2 hydroxyethyl) n phenyl benzeneacetamide; alpha intermedin; amphotericin B; arbutin; ascorbic acid; benzeneacetamide derivative; catechol; copper; DNA methyltransferase; dopachrome tautomerase; givinostat; histone deacetylase; kojic acid; melanin; melanocortin 1 receptor; messenger RNA; microphthalmia associated transcription factor; monophenol monooxygenase; n hydroxy 1 ((4 methoxyphenyl)methyl) 1H indole 6 carboxamide); n hydroxy 3 (3 (hydroxyamino) 3 oxo 1 propen 1 yl benzamide); nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase; stress activated protein kinase; transcription factor; tyrosinase related protein 1; unclassified drug; zinc; antineoplastic agent; benzeneacetamide derivative; drug; melanin; microphthalmia associated transcription factor; Mitf protein, mouse; monophenol monooxygenase; animal cell; animal experiment; Article; B16-F10 cell line; cell viability; chelation; chemoluminescence; controlled study; cytotoxicity; down regulation; enzyme activity; epigenetics; gene expression; human; human cell; hyperpigmentation; IC50; melanogenesis; melanoma; melanosome; microphthalmia; mouse; mRNA expression level; MTT assay; nonhuman; nucleotide sequence; protein expression; reverse transcription polymerase chain reaction; screening; skin pigmentation; upregulation; Western blotting; animal; chemistry; experimental melanoma; genetic epigenesis; genetics; isolation and purification; metabolism; pathology; tumor cell culture | English | 2020 | 2020-07 | 10.3390/ijms21134589 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Signaling Modulations of miR-206-3p in Tooth Morphogenesis | MicroRNAs (miRNAs) are a class of naturally occurring small non-coding RNAs that post-transcriptionally regulate gene expression in organisms. Most mammalian miRNAs influence biological processes, including developmental changes, tissue morphogenesis and the maintenance of tissue identity, cell growth, differentiation, apoptosis, and metabolism. ThemiR-206-3phas been correlated with cancer; however, developmental roles of this miRNA are unclear. In this study, we examined the expression pattern and evaluated the developmental regulation ofmiR-206-3pduring tooth morphogenesis using ex-vivo culture method. The expression pattern ofmiR-206-3pwas examined in the epithelium and mesenchyme of developing tooth germ with stage-specific manners. Perturbation of the expression ofmiR-206-3pclearly altered expression patterns of dental-development-related signaling molecules, includingAxin2, Bmp2, Fgf4, Lef1andShh. The gene expression complemented with change in cellular events including, apoptosis and proliferation which caused altered crown and pulp morphogenesis in renal-capsule-calcified teeth. Especially, mislocalization of beta-Catenin and SMAD1/5/8 were observed alongside dramatic alterations in the expression patterns ofFgf4andShh. Overall, our data suggest that themiR-206-3pregulate the cellular physiology during tooth morphogenesis through modulation of the Wnt, Bmp, Fgf, and Shh signaling pathways to form proper tooth pulp and crown. | Neupane, Sanjiv; Aryal, Yam Prasad; Kim, Tae-Young; Yeon, Chang-Yeol; An, Chang-Hyeon; Kim, Ji-Youn; Yamamoto, Hitoshi; Lee, Youngkyun; Sohn, Wern-Joo; Kim, Jae-Young | Kyungpook Natl Univ, Sch Dent, Dept Biochem, Daegu 41940, South Korea; SUNY Stony Brook, Dept Biochem & Cell Biol, Stony Brook, NY 11794 USA; Kyungpook Natl Univ, Sch Dent, Dept Oral & Maxillofacial Radiol, Daegu 41940, South Korea; Gachon Univ, Coll Hlth Sci, Dept Dent Hyg, Incheon 21936, South Korea; Tokyo Dent Coll, Dept Histol & Dev Biol, Tokyo 1010061, Japan; Daegu Haany Univ, Premajor Cosmet & Pharmaceut, Gyongsan 38610, South Korea | ; Kim, Ji-Youn/A-5779-2017 | 56183800400; 57202611163; 57208461628; 57211854259; 17134437600; 57157491000; 55725330600; 36062942200; 44161404800; 56812734700 | sanjiv.knu@gmail.com;yamaryal@yahoo.com;tae09290@daum.net;yhs2669@naver.com;chan@knu.ac.kr;hoho6434@gachon.ac.kr;hyamamoto@tdc.ac.jp;ylee@knu.ac.kr;undine75@gmail.com;jykim91@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 21 | 15 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 5.924 | 22.5 | 0.43 | 2025-06-25 | 9 | 9 | epigenetic regulation; tooth crown formation; epithelial-mesenchymal interactions; signaling regulations | PRIMARY ENAMEL KNOT; ODONTOBLAST DIFFERENTIATION; CATENIN; EXPRESSION; MICRORNAS; REARRANGEMENT; MESENCHYME; KINASE; GENES; ROLES | Epigenetic regulation; Epithelial-mesenchymal interactions; Signaling regulations; Tooth crown formation | Animals; Gene Expression Regulation, Developmental; Mice; Mice, Inbred ICR; MicroRNAs; Organogenesis; Tooth; Wnt Signaling Pathway; axin; axin2; beta catenin; bone morphogenetic protein 2; fibroblast growth factor 4; lymphoid enhancer factor 1; microRNA; microRNA 206 3p; peptides and proteins; Smad1 protein; Smad5 protein; Smad8 protein; sonic hedgehog protein; unclassified drug; microRNA; Mirn206 microRNA, mouse; adult; animal experiment; animal model; animal tissue; apoptosis; Article; cell function; cell proliferation; controlled study; embryo; epigenetics; epithelial mesenchymal transition; histology; immunohistochemistry; in situ hybridization; morphogenesis; mouse; nonhuman; pulp morphogenesis; real time polymerase chain reaction; shh signaling; signal transduction; tooth crown formation; tooth development; TUNEL assay; Wnt signaling; animal; embryology; gene expression regulation; genetics; Institute for Cancer Research mouse; metabolism; organogenesis; tooth; Wnt signaling | English | 2020 | 2020-08 | 10.3390/ijms21155251 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | The Crucial Role of Xanthine Oxidase in CKD Progression Associated with Hypercholesterolemia | In the present study, we investigated the effects of xanthine oxidase (XO) inhibition on cholesterol-induced renal dysfunction in chronic kidney disease (CKD) mice, and in low-density lipoprotein (LDL)-treated human kidney proximal tubule epithelial (HK-2) cells. ApoE knockout (KO) mice underwent uninephrectomy to induce CKD, and were fed a normal diet or high-cholesterol (HC) diet along with the XO inhibitor topiroxostat (1 mg/kg/day). HK-2 cells were treated with LDL (200 mu g/mL) and topiroxostat (5 mu M) or small interfering RNA against xanthine dehydrogenase (siXDH; 20 nM). In uninephrectomized ApoE KO mice, the HC diet increased cholesterol accumulation, oxidative stress, XO activity, and kidney damage, while topiroxostat attenuated the hypercholesterolemia-associated renal dysfunction. The HC diet induced cholesterol accumulation by regulating the expressions of genes involved in cholesterol efflux (Nr1h3 and Abca1) and synthesis (Srebf2 and Hmgcr), which was reversed by topiroxostat. Topiroxostat suppressed the expressions of genes related to hypercholesterolemia-associated inflammation and fibrosis in the unilateral kidney. LDL stimulation evoked changes in the cholesterol metabolism, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, and NF-kappa B pathways in HK-2 cells, which were mitigated by XO inhibition with topiroxostat or siXDH. These findings suggest that XO inhibition exerts renoprotective effects against hypercholesterolemia-associated kidney injury. XO could be a novel therapeutic target for hypercholesterolemia-associated kidney injury in uninephrectomized patients. | Kim, You-Jin; Oh, Se-Hyun; Ahn, Ji-Sun; Yook, Ju-Min; Kim, Chan-Duck; Park, Sun-Hee; Cho, Jang-Hee; Kim, Yong-Lim | Kyungpook Natl Univ Hosp, Div Nephrol, Daegu 41944, South Korea; Kyungpook Natl Univ, Cell & Matrix Res Inst, Daegu 41944, South Korea; Kyungpook Natl Univ, Sch Med, Dept Internal Med, Daegu 41944, South Korea | Cho, Jang-hee/ABD-3534-2020; Park, Sun-Hee/LMN-0033-2024; Kim, Yong-Lim/AGK-3172-2022 | 57190286137; 56053033900; 57191631694; 35110084800; 8558530700; 7501831741; 7403536291; 55633533600 | pinkqic1004@naver.com;ttily@nate.com;ggumsuni@hanmail.net;jumin18@hanmail.net;drcdkim@mail.knu.ac.kr;sh-park@knu.ac.kr;jh-cho@knu.ac.kr;ylkim@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 21 | 20 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 5.924 | 22.5 | 0.8 | 2025-06-25 | 18 | 20 | chronic kidney disease; hypercholesterolemia; xanthine oxidase; inflammation; fibrosis; NF-κ B pathway | STAGE RENAL-DISEASE; NF-KAPPA-B; CHOLESTEROL EXPORT; ABCA1; INJURY; METABOLISM; UNINEPHRECTOMY; ACCUMULATION; INFLAMMATION; EXPRESSION | Chronic kidney disease; Fibrosis; Hypercholesterolemia; Inflammation; NF-κB pathway; Xanthine oxidase | Cholesterol; Disease Progression; Disease Susceptibility; Fibrosis; Humans; Hypercholesterolemia; Lipid Metabolism; Lipoproteins, LDL; Oxidative Stress; Renal Insufficiency, Chronic; Signal Transduction; Xanthine Oxidase; ABC transporter A1; acetylcysteine; cell nucleus receptor; cholesterol; hydroxymethylglutaryl coenzyme A reductase; immunoglobulin enhancer binding protein; low density lipoprotein; nuclear receptor subfamily 1 group H member 3; reactive oxygen metabolite; reduced nicotinamide adenine dinucleotide phosphate oxidase; small interfering RNA; sterol regulatory element binding protein 2; topiroxostat; unclassified drug; xanthine oxidase; animal experiment; animal model; animal tissue; apolipoprotein E knockout mouse; Article; cholesterol diet; cholesterol metabolism; chronic kidney failure; controlled study; drug effect; enzyme activity; enzyme inhibition; gene expression; HK-2 [Human kidney] cell line; human; human cell; hypercholesterolemia; in vitro study; in vivo study; kidney fibrosis; kidney injury; kidney proximal tubule; lipid storage; male; mouse; nephritis; nonhuman; oxidative stress; protein synthesis; renal protection; signal transduction; uninephrectomy; chronic kidney failure; complication; disease exacerbation; disease predisposition; fibrosis; genetics; hypercholesterolemia; lipid metabolism; metabolism; pathology | English | 2020 | 2020-10 | 10.3390/ijms21207444 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Review | The Role of Epigenomics in Osteoporosis and Osteoporotic Vertebral Fracture | Osteoporosis is a complex multifactorial condition of the musculoskeletal system. Osteoporosis and osteoporotic vertebral fracture (OVF) are associated with high medical costs and can lead to poor quality of life. Genetic factors are important in determining bone mass and structure, as well as any predisposition for bone degradation and OVF. However, genetic factors are not enough to explain osteoporosis development and OVF occurrence. Epigenetics describes a mechanism for controlling gene expression and cellular processes without altering DNA sequences. The main mechanisms in epigenetics are DNA methylation, histone modifications, and non-coding RNAs (ncRNAs). Recently, alterations in epigenetic mechanisms and their activity have been associated with osteoporosis and OVF. Here, we review emerging evidence that epigenetics contributes to the machinery that can alter DNA structure, gene expression, and cellular differentiation during physiological and pathological bone remodeling. A progressive understanding of normal bone metabolism and the role of epigenetic mechanisms in multifactorial osteopathy can help us better understand the etiology of the disease and convert this information into clinical practice. A deep understanding of these mechanisms will help in properly coordinating future individual treatments of osteoporosis and OVF. | Kim, Kyoung-Tae; Lee, Young-Seok; Han, Inbo | Kyungpook Natl Univ, Sch Med, Dept Neurosurg, Daegu 41944, South Korea; Kyungpook Natl Univ Hosp, Dept Neurosurg, Daegu 41944, South Korea; Kyungpook Natl Univ Hosp, Dept Neurosurg, Chilgok Hosp, Daegu 41944, South Korea; CHA Univ, Dept Neurosurg, Sch Med, CHA Bundang Med Ctr, Seongnam Si 13496, Gyeonggi Do, South Korea | 57201369790; 57203798682; 9338449900 | nskimkt7@gmail.com;leeys1026@hanmail.net;hanib@cha.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 21 | 24 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 5.924 | 22.5 | 0.3 | 2025-06-25 | 18 | 18 | osteoporosis; osteoporotic vertebral fracture; genetic factor; epigenetics; DNA methylation; histone modification; non-coding RNA | PROMOTES OSTEOGENIC DIFFERENTIATION; MESENCHYMAL STEM-CELLS; OSTEOBLAST DIFFERENTIATION; INHIBITS OSTEOCLASTOGENESIS; METHYLATION STATUS; DNA METHYLATION; REGULATES RUNX2; NONCODING RNAS; BONE-FORMATION; GENE | DNA methylation; Epigenetics; Genetic factor; Histone modification; Non‐coding RNA; Osteoporosis; Osteoporotic vertebral fracture | DNA Methylation; Epigenesis, Genetic; Epigenomics; Fractures, Bone; Humans; Osteoporosis; Osteoporotic Fractures; Spinal Fractures; long untranslated RNA; microRNA; untranslated RNA; bone metabolism; bone remodeling; cell differentiation; DNA methylation; DNA structure; epigenetics; fragility fracture; gene expression; histone modification; human; osteoporosis; Review; spine fracture; epigenetics; fracture; fragility fracture; genetic epigenesis; genetics; osteoporosis; pathology; physiology; procedures; spine fracture | English | 2020 | 2020-12 | 10.3390/ijms21249455 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | The Topographical Optimization of 3D Microgroove Pattern Intervals for Ligamentous Cell Orientations: In Vitro | Specific orientations of periodontal ligaments (PDLs) to tooth-root surface play an important role in offering positional stabilities of teeth, transmitting and absorbing various stresses under masticatory/occlusal loading conditions, or promoting tissue remodeling by mechanical stimulations to periodontal cells. However, it is still challenging to spatially control PDL orientations and collective PDL cell alignments using 3D scaffold architectures. Here, we investigated the optimization of scaffold topographies in order to control orientations of human PDL cells with predictability in in vitro. The 3D PDL-guiding architectures were designed by computer-aided design (CAD) and microgroove patterns on the scaffold surfaces were created with four different slice intervals such as 25.40 mu m (mu G-25), 19.05 mu m (mu G-19), 12.70 mu m (mu G-12), and 6.35 mu m (mu G-6) by the digital slicing step. After scaffold design and 3D wax printing, poly-epsilon-caprolactone (PCL) was casted into 3D printed molds and human PDL cells were cultured for 7 days. In the results, mu G-25 with low vertical resolution can angularly organize seeded cells predictably rather than mu G-6 created by the highest resolution for high surface quality (or smooth surface). Moreover, nuclear orientations and deformability were quantitatively analyzed and a significant correlation between microgroove pattern intervals and cell alignments was calculated for the topographic optimization. In conclusion, controllable microgroove intervals can specifically organize human PDL cells by 3D printing, which can create various surface topographies with structural consistence. The optimal surface topography (mu G-25) can angularly guide human PDL cells, but 6.35 mu m-thick patterns (mu G-6) showed random organization of cell collectivity. | Kim, Min Guk; Park, Chan Ho | Kyungpook Natl Univ, Grad Sch, Dept Dent Sci, Daegu 41940, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Dent Biomat, Daegu 41940, South Korea; Kyungpook Natl Univ, Inst Biomat Res & Dev, Daegu 41940, South Korea | 57026505500; 55728043300 | minguk.kim@knu.ac.kr;chanho@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1422-0067 | 21 | 24 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 5.924 | 22.5 | 0.29 | 2025-06-25 | 6 | 7 | periodontal ligament; 3D printing; biopolymer; tissue engineering; microgroove; topography; optimization; regenerative medicine; cell alignment | PERIODONTAL-LIGAMENT; STEM-CELLS; INTEGRATION | 3D printing; Biopolymer; Cell alignment; Microgroove; Optimization; Periodontal ligament; Regenerative medicine; Tissue engineering; Topography | Cells, Cultured; Humans; Mesenchymal Stem Cells; Periodontal Ligament; Printing, Three-Dimensional; Tissue Engineering; Tissue Scaffolds; biopolymer; actin filament; Article; cell elongation; cell proliferation; computer aided design; controlled study; correlational study; cytoplasm; cytoskeleton; human; human cell; human tissue; in vitro study; mechanical stimulation; nuclear shape; periodontal ligament; surface property; three dimensional printing; tissue engineering; tissue regeneration; topography; cell culture; chemistry; cytology; mesenchymal stem cell; periodontal ligament; physiology; procedures; tissue scaffold | English | 2020 | 2020-12 | 10.3390/ijms21249358 | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
페이지 이동: