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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | Article | Photovoice and community-based fieldwork of preservice geography teachers: a photovoice project exploring stakeholders’ positions on social and spatial issues | This study examines methods/measures by which instructors can understand students’ experiences and empirical achievements using photovoice. The participants in this study are preservice geography who took the “Geographical Fieldwork 2” course. During the semester course, a photovoice project to investigate the conflicts among stakeholders around the university over the construction of a mosque was conducted by participants. Thus, participants individualized geographical concepts and topics learned in the classroom (for example, discrimination and stereotypes, inclusion and exclusion, land price and redevelopment, racism, difference and diversity, and global citizenship and multicultural citizenship.) in an effective way using the photovoice project in their residents. The participants’ experiences assimilated or accommodated their schema. Thus, this study identified four positive benefits of using photovoice as a measure for geographical fieldwork. © 2024 Informa UK Limited, trading as Taylor & Francis Group. | Cho, Chul-Ki | Department of Geography Education, Kyungpook National University, Daegu, South Korea | 57221970337 | ckcho@knu.ac.kr; | International Research in Geographical and Environmental Education | INT RES GEOGR ENVIRO | 1038-2046 | 1747-7611 | ESCI | EDUCATION & EDUCATIONAL RESEARCH | 2024 | 3.1 | 12.0 | 0 | 2025-05-07 | 0 | Community-based fieldwork; experiences; geographical concepts; Photovoice; preservice geography teachers | English | Article in press | 2024 | 10.1080/10382046.2024.2400859 | 바로가기 | 바로가기 | 바로가기 | ||||||||||
| ○ | ○ | Article | Population pharmacokinetic model of rifampicin for personalized tuberculosis pharmacotherapy: Effects of SLCO1B1 polymorphisms on drug exposure | Background Rifampicin (RIF) exhibits high pharmacokinetic (PK) variability among individuals; a low plasma concentration might result in unfavorable treatment outcomes and drug resistance. This study evaluated the contributions of non- and genetic factors to the interindividual variability of RIF exposure, then suggested initial doses for patients with different weight bands. Methods This multicenter prospective cohort study in Korea analyzed demographic and clinical data, the solute carrier organic anion transporter family member 1B1 (SLCO1B1) genotypes, and RIF concentrations. Population PK modeling and simulations were conducted using nonlinear mixed-effect modeling. Results In total, 879 tuberculosis (TB) patients were divided into a training dataset (510 patients) and a test dataset (359 patients). A one-compartment model with allometric scaling for effect of body size best described the RIF PKs. The apparent clearance (CL/F) was 16.6% higher among patients in the SLCO1B1 rs4149056 wild-type group than among patients in variant group, significantly decreasing RIF exposure in the wild-type group. The developed model showed better predictive performance compared with previously reported models. We also suggested that patients with body weights of 70 kg patients receive RIF doses of 450, 600, 750, and 1050 mg/day, respectively. Conclusions Total body weight and SLCO1B1 rs4149056 genotypes were the most significant covariates that affected RIF CL/F variability in Korean TB patients. We suggest initial doses of RIF based on World Health Organization weight-band classifications. The model may be implemented in treatment monitoring for TB patients. | Hoa, Pham Quang; Kim, Hyun Kuk; Jang, Tae Won; Seo, Hyewon; Oh, Jee Youn; Min, Jinsoo; Kim, Ho Cheol; Shin, Ah Young; Jayanti, Rannissa Puspita; Hung, Tran Minh; Anh, Nguyen Ky; Ahn, Sangzin; Long, Nguyen Phuoc; Cho, Yong-Soon; Shin, Jae-Gook | Inje Univ, Ctr Personalized Precis Med TB, Coll Med, Pusan, South Korea; Inje Univ, Dept Pharmacol, Coll Med, Pusan, South Korea; Inje Univ, PharmacoGen Res Ctr, Coll Med, Pusan, South Korea; Inje Univ, Busan Paik Hosp, Dept Clin Pharmacol, Pusan, South Korea; Inje Univ, Dept Internal Med, Div Pulmonol, Haeundae Paik Hosp, Pusan, South Korea; Kosin Univ, Dept Internal Med, Pulm Div, Gospel Hosp, Pusan, South Korea; Kyungpook Natl Univ Hosp, Dept Internal Med, Div Pulm Med, Daegu, South Korea; Korea Univ, Guro Hosp, Div Pulmonol, Dept Internal Med, Seoul, South Korea; Gyeongsang Natl Univ, Changwon Hosp, Sch Med, Dept Internal Med, Chang Won, South Korea; Catholic Univ Korea, Div Pulm & Crit Care Med, Dept Internal Med, Incheon St Marys Hosp,Coll Med, Seoul, South Korea; Catholic Univ Korea, Seoul St Marys Hosp, Div Pulm & Crit Care Med, Dept Internal Med,Coll Med, Seoul, South Korea; Inje Univ, PharmacoGen Res Ctr, Dept Pharmacol & Clin Pharmacol, Coll Med, 633165,Gaegum Dong,jin Gu, Pusan 47392, South Korea; Inje Univ, Pharmaco Genom Res Ctr, Dept Pharmacol Clin Pharmacol, Coll Med, 72 Bokji Ro Busanjin Gu, Pusan 47392, South Korea | Nguyen Ky, Anh/KBP-7253-2024; Long, Nguyen Phuoc/U-4162-2019; Min, Jinsoo/D-5181-2018; Oh, Jee/AAX-7147-2020 | 57470398700; 57216619509; 7102426031; 55612130200; 54420734100; 7410138275; 57203051779; 39863339000; 57470398600; 58113258600; 56624154400; 56872252100; 57678369800; 57198432982; 56031235700 | ysncho@gmail.com;phshinjg@inje.ac.kr; | INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS | INT J ANTIMICROB AG | 0924-8579 | 1872-7913 | 63 | 2 | SCIE | INFECTIOUS DISEASES;MICROBIOLOGY;PHARMACOLOGY & PHARMACY | 2024 | 4.6 | 12.0 | 1.43 | 2025-05-07 | 3 | 2 | Tuberculosis; Ritampicin; Pharmacogenetics; Population pharmacokinetics; Therapeutic drup monitoring | SEMIMECHANISTIC MODEL; COINFECTION | Pharmacogenetics; Population pharmacokinetics; Rifampicin; Therapeutic drug monitoring; Tuberculosis | Humans; Liver-Specific Organic Anion Transporter 1; Polymorphism, Genetic; Prospective Studies; Rifampin; Tuberculosis; rifampicin; rifampicin; SLCO1B1 protein, human; solute carrier organic anion transporter 1B1; adult; aged; Article; body weight; cohort analysis; demographics; drug blood level; drug clearance; drug exposure; drug monitoring; female; gene; genotype; human; Korea; Korean (people); major clinical study; male; multicenter study; nonlinear system; personalized medicine; pharmacogenetics; pharmacokinetics; population pharmacokinetics; prospective study; single nucleotide polymorphism; SLCO1B1 gene; tuberculosis; clinical trial; genetic polymorphism; genetics | English | 2024 | 2024-02 | 10.1016/j.ijantimicag.2023.107034 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Synergistic Antimicrobial Effects of Phage vBAbₐSᵢW₉ and Antibiotics against Acinetobacter baumannii Infection | Acinetobacter baumannii is a challenging multidrug-resistant pathogen in healthcare. Phage vBAbₐSᵢW₉ (GenBank: PP146379.1), identified in our previous study, shows lytic activity against 26 (89.66%) of 29 carbapenem-resistant Acinetobacter baumannii (CRAB) strains with various sequence types (STs). It is a promising candidate for CRAB treatment; however, its lytic efficiency is insufficient for complete bacterial lysis. Therefore, this study aimed to investigate the clinical utility of the phage vBAbₐSᵢW₉ by identifying antimicrobial agents that show synergistic effects when combined with it. The A. baumannii ATCC17978 strain was used as the host for the phage vBAbₐSᵢW₉. Adsorption and one-step growth assays of the phage vBAbₐSᵢW₉ were performed at MOIs of 0.001 and 0.01, respectively. Four clinical strains of CRAB belonging to different sequence types, KBN10P04948 (ST191), LIS2013230 (ST208), KBN10P05982 (ST369), and KBN10P05231 (ST451), were used to investigate phage-antibiotic synergy. Five antibiotics were tested at the following concentration: meropenem (0.25-512 mu g/mL); colistin, tigecycline, and rifampicin (0.25-256 mu g/mL); and ampicillin/sulbactam (0.25/0.125-512/256 mu g/mL). The in vitro synergistic effect of the phage and rifampicin was verified through an in vivo mouse infection model. Phage vBAbₐSᵢW₉ demonstrated 90% adsorption to host cells in 1 min, a 20 min latent period, and a burst size of 114 PFU/cell. Experiments combining phage vBAbₐSᵢW₉ with antibiotics demonstrated a pronounced synergistic effect against clinical strains when used with tigecycline and rifampicin. In a mouse model infected with CRAB KBN10P04948 (ST191), the group treated with rifampicin (100 mu g/mL) and phage vBAbₐSᵢW₉ (MOI 1) achieved a 100% survival rate-a significant improvement over the phage-only treatment (8.3% survival rate) or antibiotic-only treatment (25% survival rate) groups. The bacteriophage vBAbₐSᵢW₉ demonstrated excellent synergy against CRAB strains when combined with tigecycline and rifampicin, suggesting potential candidates for phage-antibiotic combination therapy in treating CRAB infections. | Choi, Yoon-Jung; Kim, Shukho; Shin, Minsang; Kim, Jungmin | Kyungpook Natl Univ, Sch Med, Dept Microbiol, Daegu 37224, South Korea | ; Kim, Shukho/AGG-1087-2022 | 57220082311; 24341187900; 7401536650; 57211297681 | yjchoi8727@knu.ac.kr;shukhokim@knu.ac.kr;shinms@knu.ac.kr;minkim@knu.ac.kr; | ANTIBIOTICS-BASEL | ANTIBIOTICS-BASEL | 2079-6382 | 13 | 7 | SCIE | INFECTIOUS DISEASES;PHARMACOLOGY & PHARMACY | 2024 | 4.6 | 12.0 | 5.46 | 2025-05-07 | 7 | 8 | bacteriophage; carbapenem-resistant Acinetobacter baumannii; phage-antibiotic synergy; vB_AbaSi_W9 | bacteriophage; carbapenem-resistant Acinetobacter baumannii; phage–antibiotic synergy; vB_AbaSi_W9 | ampicillin; antibiotic agent; colistin; meropenem; rifampicin; sulbactam; sultamicillin; tigecycline; Acinetobacter baumannii; Acinetobacter baumannii infection; adsorption; animal experiment; animal model; antibacterial activity; antibiotic resistance; antibiotic sensitivity; antimicrobial activity; Article; bacteremia; bacterial count; bacterial growth; bacterial infection; bacterial strain; bacteriolysis; bacteriophage; bacterium culture; bacterium isolation; Bagg albino mouse; biofilm; bloodstream infection; body weight change; carbapenem resistant Acinetobacter baumannii; cell growth assay; colony forming unit; cytotoxicity; female; growth curve; in vitro study; in vivo study; Kaplan Meier method; lethargy; minimum inhibitory concentration; mouse; mouse model; multiplicity of infection; nonhuman; optical density; pH; phage therapy; plaque assay; plaque forming unit; survival rate; synergistic effect; temperature; thermostability; toxicity | English | 2024 | 2024-07 | 10.3390/antibiotics13070680 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Therapeutic Potential of Mangosteen Pericarp Extract-Loaded Liposomes against Superficial Skin Infection Caused by Staphylococcus pseudintermedius in a Murine Model | alpha-mangostin (alpha-MG) demonstrates antibacterial activity against Staphylococcus species. Therefore, this study aimed to explore the antibacterial activity of alpha-MG-rich mangosteen pericarp extract (MPE)-loaded liposomes against Staphylococcus isolates from companion animal skin diseases in vitro and evaluated their therapeutic potential in a murine model of superficial skin infection caused by S. pseudintermedius. alpha-MG-rich extract was purified from mangosteen pericarp and then complexed with gamma-cyclodextrin (gamma-CD), forming the inclusion complexes. Nanoliposomes containing MPE and gamma-CD complexes were prepared by adding lecithin and casein. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of MPE-loaded liposomes were determined using agar dilution and broth microdilution methods. The therapeutic potential of MPE-loaded liposomes was evaluated in vivo on tape-stripped skin lesions infected with S. pseudintermedius. Purified MPE and MPE-loaded liposomes contained 402.43 mg/g and 18.18 mg/g alpha-MG, respectively. MPE-loaded liposomes showed antibacterial activity against clinical Staphylococcus isolates in vitro but did not show antibacterial activity against Gram-negative bacterial isolates. MPE-loaded liposomes demonstrated consistent MICs and MBCs against Staphylococcus isolates. These liposomes significantly reduced bacterial numbers and lesional sizes in a superficial skin infection model. Moreover, they reconstructed the epidermal barrier in skin lesions. The therapeutic concentrations of MPE-loaded liposomes did not induce cytotoxicity in canine progenitor epidermal keratinocyte cells. In conclusion, MPE-loaded liposomes hold promise for the development of a prospective topical formulation to treat superficial pyoderma in companion animals. | Kim, Seong-Yeop; Park, Seong-Yong; Lee, Jung-Hwa; Kim, Nayeong; Oh, Ha-Na; Yoo, So-Young; Lee, Dae-Sung; Lee, Je-Chul | Kyungpook Natl Univ, Sch Med, Dept Microbiol, Daegu 41944, South Korea; Med Bio Lab Co Ltd, Seoul 08389, South Korea; Kyungpook Natl Univ, Untreatable Infect Dis Inst, Daegu 41944, South Korea | 59782772500; 57958559800; 58303148700; 57211500281; 59236972800; 57211640132; 57211643343; 25930392000 | dgsk0207@naver.com;psyseongyong@knu.ac.kr;biotech77@hanmail.net;tbc02021@knu.ac.kr;jhs2hn@naver.com;smileyoo1@naver.com;dsl2008@naver.com;leejc@knu.ac.kr; | ANTIBIOTICS-BASEL | ANTIBIOTICS-BASEL | 2079-6382 | 13 | 7 | SCIE | INFECTIOUS DISEASES;PHARMACOLOGY & PHARMACY | 2024 | 4.6 | 12.0 | 0 | 2025-05-07 | 1 | 1 | mangosteen pericarp; alpha-mangostin; Staphylococcus species; superficial pyoderma; companion animals | ALPHA-MANGOSTIN; ANTIBACTERIAL ACTIVITY | companion animals; mangosteen pericarp; Staphylococcus species; superficial pyoderma; α-mangostin | gamma cyclodextrin; liposome; mangosteen pericarp extract; plant extract; unclassified drug; animal cell; animal experiment; animal model; animal tissue; antibacterial activity; antimicrobial activity; Article; broth dilution; cell survival; cell viability; colony forming unit; controlled study; crystallization; cytotoxic T lymphocyte; cytotoxicity; Escherichia coli; female; fruit; high performance liquid chromatography; histology; mangosteen fruit; MIC90; minimum bactericidal concentration; minimum inhibitory concentration; mouse; murine model; nonhuman; Pseudomonas aeruginosa; skin infection; Staphylococcus aureus; Staphylococcus epidermidis; Staphylococcus pseudintermedius; transmission electron microscopy; wound healing | English | 2024 | 2024-07 | 10.3390/antibiotics13070612 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Review | Advances in Methane Emission Estimation in Livestock: A Review of Data Collection Methods, Model Development and the Role of AI Technologies | Simple Summary This paper explores the methane emissions from the livestock industry and their large impact on climate change, with a particular focus on cattle. It emphasizes how important it is to monitor and control methane accurately because it is a powerful greenhouse gas that accounts for 14-16% of world emissions. The study evaluates both conventional and AI-powered techniques for methane emission detection, emphasizing the significance of cattle in particular. It has been determined that region-specific formulations are required. The review discusses a number of topics, such as the methane emissions from livestock, the promise of AI technology, difficulties in gathering data, the use of methane in carbon credit programs, and current research and innovation. The review aims to improve knowledge and practices for climate change mitigation by highlighting the crucial role that accurate measurement and estimation methodologies play. It draws attention to the role that methane produced by livestock, particularly cattle, plays in climate change and stresses the need for precise measuring methods to be integrated into mitigation efforts.Abstract This review examines the significant role of methane emissions in the livestock industry, with a focus on cattle and their substantial impact on climate change. It highlights the importance of accurate measurement and management techniques for methane, a potent greenhouse gas accounting for 14-16% of global emissions. The study evaluates both conventional and AI-driven methods for detecting methane emissions from livestock, particularly emphasizing cattle contributions, and the need for region-specific formulas. Sections cover livestock methane emissions, the potential of AI technology, data collection issues, methane's significance in carbon credit schemes, and current research and innovation. The review emphasizes the critical role of accurate measurement and estimation methods for effective climate change mitigation and reducing methane emissions from livestock operations. Overall, it provides a comprehensive overview of methane emissions in the livestock industry by synthesizing existing research and literature, aiming to improve knowledge and methods for mitigating climate change. Livestock-generated methane, especially from cattle, is highlighted as a crucial factor in climate change, and the review underscores the importance of integrating precise measurement and estimation techniques for effective mitigation. | Ghassemi Nejad, Jalil; Ju, Mun-Su; Jo, Jang-Hoon; Oh, Kyung-Hwan; Lee, Yoon-Seok; Lee, Sung-Dae; Kim, Eun-Joong; Roh, Sanggun; Lee, Hong-Gu | Konkuk Univ, Sanghuh Coll Life Sci, Dept Anim Sci & Technol, Seoul 05029, South Korea; Hankyong Natl Univ, Sch Biotechnol, Anseong 17579, South Korea; Hankyong Natl Univ, Ctr Genet Informat, Anseong 17579, South Korea; Rural Dev Adm, Natl Inst Anim Sci, Anim Nutr & Physiol Div, Wonju 55365, South Korea; Kyungpook Natl Univ, Dept Anim Sci, Sangju 37224, South Korea; Tohoku Univ, Grad Sch Agr Sci, Sendai 9808572, Japan | ; Oh, Kyunghwan/KVA-8714-2024; Kim, Eun Joong/HGF-1639-2022; Nejad, Jalil/V-4645-2019; ROH, SANGGUN/AFT-6087-2022 | 55600287700; 58854718600; 57217207224; 58880001600; 42861677100; 57192503342; 57213620881; 7004951490; 35074370700 | jalilgh@konkuk.ac.kr;ju2139@konkuk.ac.kr;godandthegod@konkuk.ac.kr;animal0502@konkuk.ac.kr;yoonseok95@hknu.ac.kr;leesd@korea.kr;ejkim2011@knu.ac.kr;sangun.roh@tohoku.ac.jp;hglee66@konkuk.ac.kr; | ANIMALS | ANIMALS-BASEL | 2076-2615 | 14 | 3 | SCIE | AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES | 2024 | 2.7 | 12.1 | 6.61 | 2025-05-07 | 16 | 19 | methane emission estimation; model making and AI technologies | SULFUR-HEXAFLUORIDE TRACER; INDIVIDUAL DAIRY-COWS; ENTERIC METHANE; RESPIRATION CHAMBERS; STATISTICAL-MODELS; PREDICTION; CATTLE; FARM; GREENFEED; SYSTEM | methane emission estimation; model making and AI technologies | methane; sulfur hexafluoride; tracer; artificial intelligence; artificial neural network; climate change mitigation; dairy cattle; data collection method; greenhouse gas; Holstein cattle; livestock; methane emission; nonhuman; particulate matter; Review; ruminant; training | English | 2024 | 2024-02 | 10.3390/ani14030435 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Assessing Post-Vaccination Seroprevalence and Enhancing Strategies for Lumpy Skin Disease Vaccination in Korean Cattle | Simple Summary: Lumpy skin disease (LSD) caused by the LSD virus (LSDV) severely impacts the economy of a country and its cattle industry. After the first outbreak in October 2023, South Korea decided to vaccinate cattle nationwide. This study specifically investigated the efficacy of the vaccination campaign against lumpy skin disease (LSD) and analyzed the seropositivity rates of LSD antibodies 2-3 months after vaccination to provide guidance regarding future vaccination strategies and biosecurity measures. In total, 3910 cattle were tested from four Korean provinces, with an overall seropositivity rate of 30.59 percent. Dairy cattle had higher seropositivity rates (42.97%) than Korean native cattle (29.21%). The availability of appropriate restraining facilities and vaccination methods has an influence on vaccine effectiveness. Although vaccination produces an adequate immune response, upgrading vaccination procedures, such as strengthening restraint facilities and employing professionals, can improve outcomes even more. This study offers useful insights into how to improve LSD control strategies in Korea and other affected countries. Lumpy skin disease (LSD), caused by the LSD virus (LSDV), a dsDNA virus of the genus Capripoxvirus, represents a significant cross-border infectious threat, particularly impacting cattle and water buffaloes through transmission by blood-feeding insects. Traditionally endemic to Southern Africa, LSD has rapidly spread over the past decade through the Middle East to Eastern Europe and China, reaching Korea in October 2023. This outbreak prompted a nationwide vaccination campaign, addressing both the disease's severe economic impact and its status as a notifiable disease under the World Organisation for Animal Health. This study assesses the seropositivity of the LSD vaccine in cattle across four Korean provinces 2-3 months post-vaccination, aiming to inform improvements in biosecurity and vaccination strategies. Overall, 30.59% of the cattle tested (1196 out of 3910) exhibited positive antibody responses, comparable to international post-vaccination findings. Analysis further revealed differences in the antibody positivity between farm types and management practices. Specifically, farms where vaccines were administered by veterinarians showed no significant difference in antibody positivity between Korean native cattle and dairy cattle, regardless of the presence of restraint facilities. However, on farms where vaccinations were conducted by the owners, dairy cattle demonstrated a higher seropositivity (43.30 +/- 33.39%) compared to Korean native cattle (21.97 +/- 20.79%) in the absence of restraint facilities. Further comparisons underscored the impact of restraint facilities on vaccination efficacy, with dairy farms generally achieving higher antibody positivity (29.43 +/- 30.61%) than farms with Korean native cattle (23.02 +/- 23.33%) (p < 0.05), suggesting that consistent vaccine delivery methods enhance immunogenic responses. Contrarily, no significant difference was noted in antibody positivity between large- and small-scale farms, indicating that farm size did not notably impact the effectiveness of the vaccinator. These findings emphasize that while current vaccines are sufficiently inducing immunity, enhancing vaccination strategies, particularly through trained personnel and improved restraint facilities, is crucial. This study's insights into the impact of vaccination and farm management practices provide valuable guidance for refining LSD control measures in Korea and potentially other affected regions. | Kim, Geun-Ho; Yoo, Dae-Sung; Chu, Keum-Suk; Cho, Eun-Hyo; Wi, Seung-Il; Song, Kyung-Ok; Ra, Do Kyung; Kim, Woo H.; Park, Choi-Kyu; Tark, Dongseob; Oh, Yeonsu; Cho, Ho-Seong | Jeonbuk Natl Univ, Coll Vet Med & Biosafety Res Inst, Biosafety Res Inst, Iksan 54596, South Korea; Jeollabuk do Inst Livestock & Vet Res, Coll Vet Med, Jangsu 55632, South Korea; Chonnam Natl Univ, Coll Vet Med, Gwangju 61186, South Korea; Jeollanamdo Vet Serv Lab, Gangjin 59213, South Korea; Anim Hlth Div, Jeju 63344, South Korea; Incheon Metropolitan City Inst Hlth & Environm, Incheon 22320, South Korea; Gyeongsang Natl Univ, Coll Vet Med, Jinju 52828, South Korea; Gyeongsang Natl Univ, Inst Anim Med, Jinju 52828, South Korea; Kyungpook Natl Univ, Coll Vet Med, Anim Dis Intervent Ctr, Daegu 41566, South Korea; Jeonbuk Natl Univ, Korea Zoonosis Res Inst, Iksan 54531, South Korea; Kangwon Natl Univ, Coll Vet Med, Chunchon 24341, South Korea; Kangwon Natl Univ, Inst Vet Sci, Chunchon 24341, South Korea | Kim, Woong/E-7471-2012; CHO, Ho-Seong/K-8386-2015; Kim, Geun-Ho/HNJ-6602-2023; Cho, Ho-Seong/K-8386-2015 | 59425364200; 56573450200; 59425938600; 59425364300; 59425822700; 57834246400; 59425938700; 55694027500; 24768064900; 36874709300; 57188664571; 12765051200 | kkh2701@naver.com;shanuar@chonnam.ac.kr;chuks1103@korea.kr;ddaeng3@korea.kr;wsi3866@korea.kr;songko76@korea.kr;rara9292@korea.kr;woohyun.kim@gnu.ac.kr;parkck@knu.ac.kr;tarkds@jbnu.ac.kr;yeonoh@kangwon.ac.kr;hscho@jbnu.ac.kr; | ANIMALS | ANIMALS-BASEL | 2076-2615 | 14 | 22 | SCIE | AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES | 2024 | 2.7 | 12.1 | 0 | 2025-05-07 | 0 | 0 | biosecurity; cattle; lumpy skin disease; lumpy skin disease vaccine; seropositivity; Korea | biosecurity; cattle; Korea; lumpy skin disease; lumpy skin disease vaccine; seropositivity | lumpy skin disease vaccine; lumpyvax; unclassified drug; virus vaccine; agricultural land; animal experiment; Article; biosecurity; Capripoxvirus; controlled study; dairy cattle; disease surveillance; enzyme linked immunosorbent assay; Korea; lumpy skin disease; Lumpy skin disease virus; Markov chain Monte Carlo method; nonhuman; seroprevalence; vaccination | English | 2024 | 2024-11 | 10.3390/ani14223236 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Development and evaluation of hot-melt-extruded diquafosol tetrasodium formulations for ophthalmic inserts: A design of experiments approach | This study aimed to develop, optimize, and evaluate hot-melt-extruded ophthalmic inserts capable of sustained release of diquafosol tetrasodium (DQS) via a design of experiments approach. DQS, a tear stimulant for dry eye management, faces challenges of frequent administration and low bioavailability. The developed insert uses biodegradable polymers in varied proportions to achieve sustained release. Optimized through mixture design, the insert completely dissolved within 24 h and maintained a stable drug content, thickness, and surface pH over three months at room temperature. In vitro corneal permeation studies on excised rabbit corneas demonstrated increased bioavailability, suggesting a reduced dosing frequency compared with conventional eye drops. Therefore, this insert has potential to enhance treatment outcomes by improving patient compliance and providing sustained drug effects. | Park, Ye Jin; Kim, Dong Wuk | Kyungpook Natl Univ, Coll Pharm, Vessel Organ Interact Res Ctr VOICE, BK21 FOUR Community Based Intelligent Novel Drug D, Daegu 41566, South Korea; Kyungpook Natl Univ, Res Inst Pharmaceut Sci, Daegu 41566, South Korea | 59137247400; 57193445049 | dkim17@knu.ac.kr; | INTERNATIONAL JOURNAL OF PHARMACEUTICS | INT J PHARMACEUT | 0378-5173 | 1873-3476 | 659 | SCIE | PHARMACOLOGY & PHARMACY | 2024 | 5.2 | 12.1 | 0 | 2025-05-07 | 0 | 0 | Diquafosol tetrasodium; Ophthalmic inserts; Hot-melt extrusion; Sustained release; Biodegradable polymers | DRUG-DELIVERY; IN-VITRO; EFFICACY | Biodegradable polymers; Diquafosol tetrasodium; Hot-melt extrusion; Ophthalmic inserts; Sustained release | Administration, Ophthalmic; Animals; Biological Availability; Chemistry, Pharmaceutical; Cornea; Delayed-Action Preparations; Drug Compounding; Drug Implants; Drug Liberation; Hot Temperature; Ophthalmic Solutions; Polyphosphates; Rabbits; Uracil Nucleotides; diquafosol; eye drops; macrogol 6000; polymer; diquafosol; eye drops; polyphosphate; pyrimidine nucleotide; adsorption; adult; animal experiment; animal model; animal tissue; Article; biocompatibility; controlled study; cornea; cornea permeability; diffusion; drug bioavailability; drug effect; drug formulation; drug solubility; ex vivo study; experimental design; Fourier transform infrared spectroscopy; glass transition temperature; high performance liquid chromatography; hot melt extrusion; in vitro study; Leporidae; male; moisture; nonhuman; patient compliance; pH; relative humidity; scanning electron microscopy; sustained drug release; thermostability; torque; treatment outcome; viscosity; X ray powder diffraction; animal; bioavailability; chemistry; cornea; delayed release formulation; drug formulation; drug implant; drug release; high temperature; intraocular drug administration; medicinal chemistry; metabolism; procedures | English | 2024 | 2024-06-25 | 10.1016/j.ijpharm.2024.124249 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Effect of Oligo-Fucoidan, Fucoxanthin, and L-Carnitine on Chronic Kidney Disease in Dogs: A Retrospective Study | Simple Summary Chronic kidney disease (CKD) is common in old dogs and cats. Patients with CKD have impaired renal structures and decreased renal function. Because renal degeneration is generally irreversible, treatment of CKD is focused mainly on conserving the remaining renal function. In this study, we retrospectively evaluated the effects of oligo-fucoidan, fucoxanthin, and L-carnitine in canine CKD patients. The supplements were supplied for 6 months and showed a reno-protective effect, consistent with previous animal model studies. Based on our results, the combination of oligo-fucoidan, fucoxanthin, and L-carnitine has the potential to delay the progression of canine CKD and be used as an adjuvant therapy.Abstract Chronic kidney disease (CKD) commonly occurs in old dogs and cats. Oligo-fucoidan, fucoxanthin, and L-carnitine (OFL) compounds have a variety of reno-protective properties, including anti-inflammatory, anti-oxidative, and anti-fibrotic effects. Because their effects have not been investigated in naturally occurring canine CKD, we examined their reno-protective activities in dog patients with CKD. A total of 50 patients (OFL, n = 28; control, n = 22) were included in the analysis. A significant difference was identified in serum blood urea nitrogen and creatinine concentrations between the control and OFL groups at 6 months. No significant difference in electrolytes was found between the groups. A significant difference was identified in serum creatinine concentration between the control and OFL groups in azotemic (CKD IRIS stage 2-4) at 6 months. The OFL compounds showed a reno-protective effect, consistent with previous animal studies. The OFL combination can potentially delay the progression of canine CKD and be used as an adjuvant therapy. | Hong, Naeun; An, Ju-Hyun; Kim, Sung-Soo; Park, Su-Min; Lim, Ga-Hyun; Oh, Ye-In; Seo, Kyoung-Won; Youn, Hwa-Young | Seoul Natl Univ, Coll Vet Med, Dept Vet Clin Sci, Lab Vet Internal Med, Seoul 08826, South Korea; Kangwon Natl Univ, Coll Vet Med, Dept Vet Emergency & Crit Care Med, Chuncheon Si 24341, South Korea; VIP Anim Med Ctr KR, Seoul 02830, South Korea; Kyungpook Natl Univ, Coll Vet Med, Dept Vet Internal Med, Daegu 41566, South Korea | Seo, Kwang/AAB-4821-2020; Kim, Sung-Soo/AFP-2188-2022 | 59171515200; 57206839650; 57410126300; 57211909684; 58132315300; 36466903200; 8833507700; 7102027331 | naeun1001@snu.ac.kr;moonlit0816@naver.com;ilovekh0@hanmail.net;ssumin94@snu.ac.kr;rkgus0724@snu.ac.kr;imyein5@knu.ac.kr;kwseo@snu.ac.kr;hyyoun@snu.ac.kr; | ANIMALS | ANIMALS-BASEL | 2076-2615 | 14 | 11 | SCIE | AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES | 2024 | 2.7 | 12.1 | 1.08 | 2025-05-07 | 0 | 1 | chronic kidney disease; reno-protective; oligo-fucoidan; fucoxanthin; L-carnitine | chronic kidney disease; fucoxanthin; L-carnitine; oligo-fucoidan; reno-protective | antiinflammatory agent; antioxidant; calcium; carnitine; chloride; creatinine; fucoidin; fucoxanthin; lysine; methionine; nitrogen; pamidronic acid; phosphate; phosphorus; polysaccharide sulfate; potassium; probiotic agent; protein concentrate plus electrolytes; sodium; spironolactone; theophylline; urea; adverse event; Article; blood analysis; blood pressure; body weight; brain tumor; breathing rate; calcium blood level; chronic kidney failure; chronic pancreatitis; clinical article; controlled study; diagnostic test accuracy study; diet supplementation; dog; female; glomerulus filtration rate; heart rate; human; hypertension; kidney; kidney fibrosis; liver; lung edema; male; mitral valve disease; nonhuman; pneumonia; proteinuria; rectal temperature; retrospective study; serum; systolic blood pressure; ultrasound; urea nitrogen blood level; urinalysis | English | 2024 | 2024-06 | 10.3390/ani14111696 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Genomic and Pathological Characterization of Acute Hepatopancreatic Necrosis Disease (AHPND)-Associated Natural Mutant Vibrio parahaemolyticus Isolated from Penaeus vannamei Cultured in Korea | Simple Summary: Acute hepatopancreatic necrosis disease (AHPND) is a plasmid-encoded PirA/B toxin mediated bacterial disease that causes significant economic losses to the global shrimp industry. Since the first AHPND outbreak of Penaeus vannamei cultures in Korea in 2016, AHPND-associated Vibrio parahaemolyticus (Vp(AHPND)) is still considered a major threat to the Korean shrimp industry. Although several diagnostic methods have been developed against AHPND, several concerns have been raised about the misdiagnosis of the pathogen due to the transferability and mutation of the plasmid. In this study, we report the emergence of a natural mutant Vp(AHPND) (pirA(-), pirB(+)), which was isolated from cultured P. vannamei in Korea, and also provide its detailed genomic and pathological characteristics. Acute hepatopancreatic necrosis disease (AHPND) is one of the most important diseases in the global shrimp industry. The emergence of mutant AHPND-associated V. parahaemolyticus (Vp(AHPND)) strains has raised concerns regarding potential misdiagnosis and unforeseen pathogenicity. In this study, we report the first emergence of a type II (pirA(-), pirB(+)) natural mutant, Vp(AHPND) (strain 20-082A3), isolated from cultured Penaeus vannamei in Korea. Phenotypic and genetic analyses revealed a close relationship between the mutant strain 20-082A3 and the virulent Korean Vp(AHPND) strain 19-021-D1, which caused an outbreak in 2019. Detailed sequence analysis of AHPND-associated plasmids showed that plasmid pVp₂₀-082A3B in strain 20-082A3 was almost identical (>99.9%) to that of strain 19-021-D1. Moreover, strains 20-082A3 and 19-021-D1 exhibited the same multilocus sequence type (ST 413) and serotype (O1:Un-typeable K-serogroup), suggesting that the mutant strain is closely related to and may have originated from the virulent strain 19-021-D1. Similar to previous reports on the natural mutant Vp(AHPND), strain 20-082A3 did not induce AHPND-related symptoms or cause mortality in the shrimp bioassay. The emergence of a mutant strain which is almost identical to the virulent Vp(AHPND) highlights the need for surveillance of the pathogen prevalent in Korea. Further investigations to elucidate the potential relationship between ST 413 and recent Korean Vp(AHPND) isolates are needed. | Kim, Ye Bin; Park, Seon Young; Jeon, Hye Jin; Kim, Bumkeun; Kwon, Mun-Gyeong; Kim, Su-Mi; Han, Jee Eun; Kim, Ji Hyung | Gachon Univ, Coll Bionano Technol, Dept Food Sci & Biotechnol, Seongnam 13120, South Korea; Seoul Natl Univ, Coll Vet Med, Lab Aquat Biomed, Seoul 08826, South Korea; Seoul Natl Univ, Res Inst Vet Sci, Seoul 08826, South Korea; Kyungpook Natl Univ, Coll Vet Med, Inst Vet Biomed Sci, Daegu 41566, South Korea; Natl Fishery Prod Qual Management Serv, Aquat Dis Control Div, Busan 46083, South Korea | Kim, Ji Hyung/B-7321-2011 | 58639041000; 57208480038; 57212193058; 57225056167; 36508778600; 57320586000; 57214671240; 57225000204 | zxc5620@gachon.ac.kr;lovesun139@snu.ac.kr;jhj1125@knu.ac.kr;aam_kim@knu.ac.kr;mgkwon@korea.kr;sumikim@korea.kr;jehan@knu.ac.kr;kzh81@gachon.ac.kr; | ANIMALS | ANIMALS-BASEL | 2076-2615 | 14 | 19 | SCIE | AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES | 2024 | 2.7 | 12.1 | 1.08 | 2025-05-07 | 1 | 1 | Vp(AHPND); ST 413; O1: non-typeable K; shrimp bioassay | CAUSATIVE AGENT; STRAINS; DATABASE; PLASMID; GENES; AHPND | O1: non-typeable K; shrimp bioassay; ST 413; strain 19-021-D1; Vp<sub>AHPND</sub> | amikacin; ampicillin; azithromycin; cefepime; cefotaxime; cefoxitin; ceftazidime; chloramphenicol; cholera toxin transcriptional activator; ciprofloxacin; cotrimoxazole; doxycycline; levofloxacin; photorhabdus insect related toxin A; photorhabdus insect related toxin B; piperacillin; protein; protirelin; pVA1 like plasmid protein; sultamicillin; tetracycline; thermolabile hemolysin gene; thermostable direct hemolysin gene; unclassified drug; acute hepatopancreatic necrosis disease; animal experiment; animal tissue; antibiotic sensitivity; Article; bacterial colonization; bacterial strain; bacterium identification; bacterium isolation; biosurveillance; controlled study; disk diffusion; DNA isolation; gene mutation; gene sequence; genomics; hemolysis; histopathology; minimum inhibitory concentration; multilocus sequence typing; multiplex polymerase chain reaction; nonhuman; nucleotide sequence; pancreas necrosis; pathogenicity; pathology; Penaeus vannamei; Vibrio parahaemolyticus; whole genome sequencing | English | 2024 | 2024-10 | 10.3390/ani14192788 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Histological and Molecular Biological Changes in Canine Skin Following Acute Radiation Therapy-Induced Skin Injury | Simple Summary This study focused on understanding how radiation therapy, a common treatment for cancer, affects the skin of dogs. While radiation is effective at destroying cancer cells, it can also cause damage to healthy skin, leading to various side effects like redness, peeling, changes in skin color, and sores. Over nine weeks, we monitored these skin changes in dogs, observing that radiation led to increased inflammation and stress in the skin cells, as well as significant disruptions in how skin cells grow and heal. We also noticed changes in specific proteins and genes related to skin inflammation, healing, and cell death. These findings help us better understand how radiation therapy impacts the skin and provide valuable information for managing these side effects in dogs.Abstract Radiation therapy is a crucial cancer treatment, but it can damage healthy tissues, leading to side effects like skin injuries and molecular alterations. This study aimed to elucidate histological and molecular changes in canine skin post-radiation therapy (post-RT) over nine weeks, focusing on inflammation, stem cell activity, angiogenesis, keratinocyte regeneration, and apoptosis. Four male beagles received a cumulative radiation dose of 48 Gy, followed by clinical observations, histological examinations, and an RT-qPCR analysis of skin biopsies. Histological changes correlated with clinical recovery from inflammation. A post-RT analysis revealed a notable decrease in the mRNA levels of Oct4, Sox2, and Nanog from weeks 1 to 9. VEGF 188 levels initially saw a slight increase at week 1, but they had significantly declined by week 9. Both mRNA and protein levels of COX-2 and Keratin 10 significantly decreased over the 9 weeks following RT, although COX-2 expression surged in the first 2 weeks, and Keratin 10 levels increased at weeks 4 to 5 compared to normal skin. Apoptosis peaked at 2 weeks and diminished, nearing normal by 9 weeks. These findings offer insights into the mechanisms of radiation-induced skin injury and provide guidance for managing side effects in canine radiation therapy. | Lee, Sang-Yun; Hwang, Gunha; Choi, Moonyeong; Jo, Chan-Hee; Oh, Seong-Ju; Jin, Yeung Bae; Lee, Won-Jae; Rho, Gyu-Jin; Lee, Hee Chun; Lee, Sung-Lim; Hwang, Tae Sung | Gyeongsang Natl Univ, Coll Vet Med, Dept Pharmacol & Toxicol, Jinju 52828, South Korea; Yangsan S Anim Canc Ctr, Yangsan 50638, South Korea; Kyungpook Natl Univ, Coll Vet Med, Daegu 41566, South Korea; Gyeongsang Natl Univ, Res Inst Life Sci, Jinju 52828, South Korea; Gyeongsang Natl Univ, Inst Anim Med, Coll Vet Med, Jinju 52828, South Korea | ; lee, sang/Q-4650-2019; Kim, Gi/AAK-3377-2021; Hwang, Tae Sung/HHC-4238-2022 | 57216913305; 57220815750; 57194007368; 57221808828; 57220159013; 35344928700; 57205486455; 6603725057; 25924416700; 16678898100; 56959822600 | sy_lee@gnu.ac.kr;hgh3634@gmail.com;vetmoon7@gmail.com;ch_jo@gnu.ac.kr;osj414@gnu.ac.kr;ybjin@gnu.ac.kr;iamcyshd@knu.ac.kr;jinrho@gnu.ac.kr;lhc@gnu.ac.kr;sllee@gnu.ac.kr;hwangts@gnu.ac.kr; | ANIMALS | ANIMALS-BASEL | 2076-2615 | 14 | 17 | SCIE | AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES | 2024 | 2.7 | 12.1 | 0 | 2025-05-07 | 0 | 0 | canine; clinical change; quantitative real-time PCR; radiation therapy-induced injury | ENDOTHELIAL GROWTH-FACTOR; MESSENGER-RNA EXPRESSION; COX-2 EXPRESSION; GENE-EXPRESSION; STEM-CELLS; VEGF; IRRADIATION; CANCER; PROLIFERATION; ANGIOGENESIS | canine; clinical change; quantitative real-time PCR; radiation therapy-induced injury | cyclooxygenase 2; cytokeratin 10; hypoxanthine phosphoribosyltransferase; messenger RNA; octamer transcription factor 4; propofol; transcription factor NANOG; transcription factor Sox2; vasculotropin; alopecia; angiogenesis; animal experiment; animal tissue; apoptosis; Article; beagle; cancer therapy; Canis; carcinogenesis; cell activity; cell death; cell proliferation; chemexfoliation; controlled study; dermatitis; desquamation; DNA methylation; dog; endotracheal intubation; epidermis hyperplasia; epilation; healing; histology; hyperkeratosis; hyperpigmentation; inflammation; irradiation; keratinocyte; male; microscopy; molecular biology; nonhuman; radiation dermatitis; radiation injury; real time reverse transcription polymerase chain reaction; skin biopsy; skin injury; skin redness; stem cell; TUNEL assay | English | 2024 | 2024-09 | 10.3390/ani14172505 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Identification of Reference Gene for Quantitative Gene Expression in Early-Term and Late-Term Cultured Canine Fibroblasts Derived from Ear Skin | Simple Summary This study aimed to identify stable reference genes in early-passage and late-passage cultured canine skin fibroblasts. The early-passage fibroblasts retained their spindle-shaped morphology, exhibited a short doubling time, and had low beta-galactosidase activity. In contrast, the late-passage fibroblasts displayed an elongated morphology, a prolonged doubling time, and elevated beta-galactosidase activity. To assess the stability of the reference genes, the Ct values obtained using qRT-PCR were analyzed using three algorithms: geNorm, NormFinder, and BestKeeper. As a result, HPRT1, YWHAZ, and GUSB were identified as the most stable reference genes across all three algorithms in canine skin fibroblasts. When comparing early-passage to late-passage fibroblasts, the normalization of Vimentin expression using both stable and unstable reference genes showed a decrease in late-passage cells. Although the use of less stable reference genes did not result in a significant difference in Vimentin expression, the use of stable reference genes revealed a significant difference. This study provides a foundation for the further application of RT-qPCR in the gene expression analysis of long-term expanded canine skin fibroblasts.Abstract Fibroblasts are cells that reside within the fibrous or loose connective tissues of most mammalian organs. For research purposes, fibroblasts are often subjected to long-term culture under defined conditions, during which their properties can significantly change. It is essential to understand and document these changes to obtain reliable outcomes. For the quantification of specific gene expressions, the most reliable and widely used technique is quantitative real-time polymerase chain reaction (qRT-PCR). Here, we assessed the impact of a reference gene's stability on a qRT-PCR analysis of long-term cultured canine skin fibroblasts. After successfully isolating the fibroblasts from canine skin tissues, they were cultured and evaluated for proliferation and beta-galactosidase activity at different passage numbers. With extended culture, the fibroblasts showed a long doubling time and elevated beta-galactosidase activity. Using three widely used algorithms, geNorm, Normfinder, and Bestkeeper, we identified HPRT1, YWHAZ, and GUSB as the most stable reference genes for both early- and late-passage fibroblasts. Conventional reference genes such as GAPDH were found to be less stable than those genes. The normalization of Vimentin by the stable genes showed statistical differences, whereas normalization by an unstable gene did not. Collectively, this study indicates that using stable reference genes is essential for accurately and reliably measuring gene expression in both early- and late-passage fibroblasts. These findings provide valuable insights into internal controls for gene expression studies and are expected to be utilized for analyzing gene expression patterns in molecular biology research. | Lee, Sang-Yun; Jeong, Yeon-Woo; Choe, Yong-Ho; Oh, Seong-Ju; Miah, Rubel; Lee, Won-Jae; Lee, Sung-Lim; Bok, Eun-Yeong; Yoo, Dae-Sung; Son, Young-Bum | Gyeongsang Natl Univ, Coll Vet Med, Dept Theriogenol & Biotechnol, Jinju 52628, South Korea; Korea Res Inst Biosci & Biotechnol KRIBB, Stem Cell Convergence Res Ctr, Daejeon 34141, South Korea; Joongbu Univ, Dept Compan Anim & Anim Resources Sci, Geumsan 32713, South Korea; Chonnam Natl Univ, Coll Vet Med, Dept Obstet, 300 Yonbongdong, Gwangju 61186, South Korea; Kyungpook Natl Univ, Coll Vet Med, Dept Obstet, Daegu 41566, South Korea; Gyeongsang Natl Univ, Res Inst Life Sci, Jinju 52828, South Korea; Rural Dev Adm, Natl Inst Anim Sci, Div Anim Dis & Hlth, Wonju 55365, South Korea; Chonnam Natl Univ, Coll Vet Med, Dept Vet Epidemiol, 300 Yonbongdong, Gwangju 61186, South Korea | lee, sang/Q-4650-2019; Kim, Gi/AAK-3377-2021; bok, eun yeong/MYS-4388-2025 | 57216913305; 7202331966; 57202927556; 57220159013; 59345678500; 57205486455; 16678898100; 57221645701; 56573450200; 57197735518 | sy_lee@gnu.ac.kr;doctorj1@joongbu.ac.kr;yhchoego@gmail.com;osj414@gnu.ac.kr;rubelsau26@gmail.com;iamcyshd@knu.ac.kr;sllee@gnu.ac.kr;shanuar@jnu.ac.kr;ybson@jnu.ac.kr; | ANIMALS | ANIMALS-BASEL | 2076-2615 | 14 | 18 | SCIE | AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES | 2024 | 2.7 | 12.1 | 0 | 2025-05-07 | 0 | 0 | canine; fibroblasts; long-term expansion; qRT-PCR; reference gene | REAL-TIME; INTERNAL CONTROL; CELL-ADHESION; BETA-ACTIN; SELECTION; DIFFERENTIATION; VALIDATION; VIMENTIN; PCR | canine; fibroblasts; long-term expansion; qRT-PCR; reference gene | beta 2 microglobulin; beta actin; beta galactosidase; glyceraldehyde 3 phosphate dehydrogenase; hypoxanthine phosphoribosyltransferase; messenger RNA; ribosome protein; ribosome protein 4; ribosome protein s5; TATA binding protein; tryptophan hydroxylase; tyrosine 3 monooxygenase; unclassified drug; vimentin; animal cell; animal experiment; animal tissue; Article; cell aging; cell count; cell isolation; comparative study; controlled study; cycle threshold value; dog; enzyme activity; enzyme denaturation; fibroblast culture; gel electrophoresis; gene expression; gene identification; male; measurement accuracy; morphological trait; nonhuman; punch biopsy; real time polymerase chain reaction; reverse transcription polymerase chain reaction; RNA extraction; RNA isolation; skin fibroblast; stable expression; tumor doubling time | English | 2024 | 2024-09 | 10.3390/ani14182722 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Nationwide Survey of Vector-Borne Diseases in Rodents and Mites in Korea: Anaplasma, Ehrlichia, and Rickettsia | Simple Summary Rodents are known to carry a variety of pathogens that can cause diseases in humans, especially those transmitted through small parasites like mites. This study investigated the presence of three specific bacteria, Anaplasma, Ehrlichia, and Rickettsia, in rodents and their mites across Korea in 2022 and 2023. We found that 10.3% of the 835 rodents were infected with Anaplasma phagocytophilum, 0.5% with Ehrlichia muris, 0.2% with Ehrlichia ruminantium, and 2.9% with Rickettsia raoultii. In addition, 8.8% of 7971 mites tested positive for Anaplasma, and lower percentages of the mites tested positive for the other bacteria. Importantly, this is the first time these bacteria have been detected in rodents and mites in Korea, and in some cases, globally. Our findings highlight the need for ongoing monitoring and better systems to track these diseases to prevent future outbreaks, especially as climate and environmental changes may increase their spread. This information is vital for public health awareness and protection from vector-borne diseases.Abstract Rodents are reservoirs for zoonotic pathogens, making it essential to study both rodents and their ectoparasites. In 2022 and 2023, we investigated the spatial distribution of rodents and their mites across Korea, focusing on three vector-borne diseases (VBDs): Anaplasma, Ehrlichia, and Rickettsia. A total of 835 wild rodents were collected from 16 locations, each consisting of five distinct environmental settings (mountains, waterways, reservoirs, fields, and paddy fields), with 20 traps per setting, totaling 100 Sherman live folding traps per site. Each rodent was identified using a taxonomic key, and post-mortem examinations led to the collection of 7971 mites (498 pools), followed by PCR analysis. Among the rodents, Anaplasma phagocytophilum was detected in 10.3%, Ehrlichia muris in 0.5%, Ehrlichia ruminantium in 0.2%, and Rickettsia raoultii in 2.9%. In mites, A. phagocytophilum was found in 8.8%, E. muris in 0.2%, R. raoultii in 0.2%, R. endosymbiont in 1.6%, and R. australis in 1.2%. This study marks the first detection of E. muris and R. raoultii in Korean rodents and the first global discovery of E. ruminantium in rodents. The detection of multiple pathogens in mites worldwide highlights the importance of continuous VBD monitoring to mitigate public health risks. | Kim, Beoul; Lee, You-Jeong; Kwak, Dongmi; Seo, Min-Goo | Kyungpook Natl Univ, Coll Vet Med, Daegu 41566, South Korea; Kyungpook Natl Univ, Inst Vet Biomed Sci, 80 Daehak ro, Daegu 41566, South Korea | Seo, Min-Goo/NQF-4335-2025 | 59343608800; 59343760600; 7007148758; 53982155300 | kbjjhnm@naver.com;wowgirlsgood@naver.com;dmkwak@knu.ac.kr;koreasmg@knu.ac.kr; | ANIMALS | ANIMALS-BASEL | 2076-2615 | 14 | 20 | SCIE | AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES | 2024 | 2.7 | 12.1 | 0 | 2025-05-07 | 1 | 1 | wild rodents; mites; Anaplasma; Ehrlichia; Rickettsia | MOLECULAR-DETECTION; HIGH PREVALENCE; TICKS; PHAGOCYTOPHILUM; INFECTION; PATHOGENS; CATTLE; SPP.; IDENTIFICATION; EPIDEMIOLOGY | Anaplasma; Ehrlichia; mites; Rickettsia; wild rodents | polyvinyl alcohol; RNA 16S; Anaplasma; Anaplasma phagocytophilum; Article; bioinformatics; DNA extraction; ectoparasite; Ehrlichia; Ehrlichia muris; Ehrlichia ruminantium; endosymbiont; health hazard; Korea; microbial diversity; mountain; nonhuman; nucleotide sequence; paddy field; phylogenetic tree; phylogeny; polymerase chain reaction; Rickettsia; Rickettsia raoultii; rodent; sequence alignment; vector borne disease; virus detection | English | 2024 | 2024-10 | 10.3390/ani14202950 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Prevalence and Genetic Characterization of Porcine Respiratory Coronavirus in Korean Pig Farms | Simple Summary The current epidemiology of porcine respiratory coronavirus (PRCV) in domestic pig farms in the Republic of Korea is not well understood. In this study, PRCV was found to still be present in Korean pig herds with a high seroprevalence. Genetic and phylogenetic analyses of PRCV S gene sequences suggested that Korean PRCV originated from European PRCV and has evolved in Korea. These findings will help expand knowledge about the epidemiology and genetic characteristics of PRCV in Korea.Abstract Porcine respiratory coronavirus (PRCV) is a member of the species Alphacoronavirus 1 within the genus Alphacoronavirus of the family Coronaviridae. A few studies have been conducted on the prevalence of PRCV since its first identification in 1997, but there have been no recent studies on the prevalence and genetic characterization of the virus in Korea. In this study, the seroprevalence of PRCV was determined in Korean pig farms using a commercially available TGEV/PRCV differential enzyme-linked immunosorbent assay kit. The farm-level seroprevalence of PRCV was determined to be 68.6% (48/70), similar to previous reports in Korea, suggesting that PRCV is still circulating in Korean pig herds nationwide. Among the 20 PRCV-seropositive farms tested in this study, PRCV RNAs were detected in 17 oral fluid samples (28.3%) from nine farms (45.0%), while TGEV RNAs were not detected in any sample. To investigate the genetic characteristics of Korean PRCV strains, genetic and phylogenetic analyses were conducted on PRCV spike gene sequences obtained in this study. The three Korean PRCV strains (KPRCV2401, KPRCV2402, and KPRCV2403) shared 98.5-100% homology with each other and 96.2-96.6% and 91.6-94.5% homology with European and American strains, respectively. A 224-amino acid deletion was found in the S gene of both Korean and European PRCVs but not in that of American PRCVs, suggesting a European origin for Korean PRCVs. Phylogenetic analysis showed that Korean PRCVs are more closely related to European PRCVs than American PRCVs but clustered apart from both, suggesting that Korean PRCV has evolved independently since its emergence in Korean PRCVs. The results of this study will help expand knowledge on the epidemiology and molecular biology of PRCV currently circulating in Korea. | Kim, Ju-Han; Park, Jonghyun; Lee, Dong-Kyu; Kim, Won-Il; Lyoo, Young S.; Park, Choi-Kyu; Kim, Hye-Ryung | Konkuk Univ, Coll Vet Med, Icheon 05029, South Korea; Swine Med Corp, Sunjin Bridge Lab, Icheon 17332, South Korea; Kyungpook Natl Univ, Inst Vet Biomed Sci, Coll Vet Med, Daegu 41566, South Korea; DIVA Bio Inc, Daegu 41519, South Korea; Jeonbuk Natl Univ, Coll Vet Med, Iksan 54596, South Korea | ; Kim, Jihan/AAE-6280-2021; Dong Kyu, Lee/HIK-3875-2022 | 59172011600; 57210160562; 55698913900; 55730056800; 35501431200; 24768064900; 57195772612 | jhkim2@sj.co.kr;parkjh@knu.ac.kr;mooninlake@dodram.co.kr;kwi0621@jbnu.ac.kr;lyoo@konkuk.ac.kr;parkck@knu.ac.kr;gpfuddl25@knu.ac.kr; | ANIMALS | ANIMALS-BASEL | 2076-2615 | 14 | 11 | SCIE | AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES | 2024 | 2.7 | 12.1 | 3.24 | 2025-05-07 | 4 | 4 | porcine respiratory coronavirus; prevalence; genetic analysis; pig farm; Republic of Korea | TRANSMISSIBLE GASTROENTERITIS VIRUS; EPIDEMIC DIARRHEA VIRUS; INFLUENZA-VIRUS; PCR; PATHOGENICITY; INFECTION; SEQUENCE; QPCR; PRCV | genetic analysis; pig farm; porcine respiratory coronavirus; prevalence; Republic of Korea | Article; enzyme linked immunosorbent assay; gene sequence; genetic analysis; knowledge; Korea; nonhuman; nucleotide sequence; phylogenetic tree; phylogeny; pig; Porcine respiratory coronavirus; prevalence; real time polymerase chain reaction; real time reverse transcription polymerase chain reaction; sequence alignment; sequence analysis; serology; seroprevalence | English | 2024 | 2024-06 | 10.3390/ani14111698 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Regulation of Colonic Inflammation and Macrophage Homeostasis of IFN-γ-Primed Canine AMSCs in Experimental Colitis in Mice | Mesenchymal stem cells (MSCs) have shown potential in treating immune-mediated diseases due to their immunomodulatory properties, which can be enhanced by priming with inflammatory cytokines like interferon-gamma (IFN-gamma). This study evaluates the therapeutic effects of IFN-gamma-primed canine adipose tissue-derived MSCs (AMSCs) in a mouse model of inflammatory bowel disease (IBD). Canine AMSCs were primed with 50 ng/mL recombinant canine IFN-gamma for 48 h, and the effects were compared to those seen in na & iuml;ve (unprimed) AMSCs. IBD was induced in mice using dextran sodium sulfate (DSS), and AMSCs were injected intraperitoneally on days 1 and 3. The mice treated with IFN-gamma-primed AMSCs showed improved clinical outcomes, including a reduced disease activity index (DAI), less body weight loss, and longer colon length compared to the mice treated with na & iuml;ve AMSCs. A histological analysis revealed less damage to the intestinal structures and reduced inflammatory cell infiltration. IFN-gamma priming led to a shift in the immune cell balance in the gut, decreasing pro-inflammatory macrophages (Ly6Chi) and increasing anti-inflammatory macrophages (Ly6Clo/MHC-IIhi). This was associated with the reduced expression of inflammatory cytokine genes (Il-1 beta, Il-6, and Il-18) and increased expression of the intestinal stem cell marker Lgr5. These findings suggest that IFN-gamma-primed AMSCs offer enhanced therapeutic potential for treating CE in veterinary medicine. | Jo, Chan-Hee; Lee, Sang-Yun; Son, Young-Bum; Lee, Won-Jae; Choe, Yong-Ho; Lee, Hyeon-Jeong; Oh, Seong-Ju; Kim, Tae-Seok; Hong, Chae-Yeon; Lee, Sung-Lim; Rho, Gyu-Jin | Gyeongsang Natl Univ, Coll Vet Med, Dept Anat, Jinju 52828, South Korea; Chonnam Natl Univ, Coll Vet Med, Dept Obstet, 300 Yonbongdong, Gwangju 61186, South Korea; Kyungpook Natl Univ, Coll Vet Med, Daegu 41566, South Korea; Gyeongsang Natl Univ, Res Inst Life Sci, Jinju 52828, South Korea | 57221808828; 57216913305; 57197735518; 57205486455; 57202927556; 57202973938; 57220159013; 58412692900; 58411924300; 16678898100; 6603725057 | ch_jo@gnu.ac.kr;sy_lee@gnu.ac.kr;ybson@jnu.ac.kr;iamcyshd@knu.ac.kr;yhchoego@gmail.com;feellove97@naver.com;osj414@gnu.ac.kr;kiminfaith@naver.com;xoojoox8@gmail.com;sllee@gnu.ac.kr;jinrho@gnu.ac.kr; | ANIMALS | ANIMALS-BASEL | 2076-2615 | 14 | 22 | SCIE | AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES | 2024 | 2.7 | 12.1 | 0 | 2025-05-07 | 0 | 0 | canine; mesenchymal stem cells; IFN-gamma; inflammatory bowel disease; immunomodulation | MESENCHYMAL STEM-CELLS; BOWEL-DISEASE; INTERFERON-GAMMA; IMMUNOMODULATION | canine; IFN-γ; immunomodulation; inflammatory bowel disease; mesenchymal stem cells | CD11b antigen; chemokine receptor CX3CR1; complementary DNA; dextran sulfate; diaminobenzidine; gamma interferon; glycoprotein p 15095; interleukin 17; interleukin 18; interleukin 1beta; interleukin 4; interleukin 6; peroxidase; adipose-derived mesenchymal stem cell; animal model; animal tissue; Article; body weight loss; C57BL 6 mouse; CD4+ T lymphocyte; cell differentiation; cell infiltration; cell permeabilization; cell suspension; colitis; confocal microscopy; dendritic cell; disease activity; disease severity; enteropathy; flow cytometry; fluorescence intensity; histology; histopathology; homeostasis; immunocompetent cell; immunofluorescence; immunohistochemistry; immunotherapy; inflammatory bowel disease; macrophage; male; mesenchymal stem cell; mouse; neutralization test; nonhuman; phenotype; real time polymerase chain reaction; RNA extraction; Th1 cell; upregulation | English | 2024 | 2024-11 | 10.3390/ani14223283 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Standardized Ileal Digestibility of Calcium and Phosphorus in Feed Ingredients for 21-Day-Old Broilers | Simple Summary Plant-based ingredients are the major constituents of poultry diets and supply a significant portion of dietary phosphorus (P). However, approximately two-thirds of the P of plant origin is bound to phytic acid, which is poorly available to monogastric animals. Broiler diets have been formulated based on non-phytate P (NPP), which might lead to the excessive use of inorganic P sources and reduced P availability for birds. Recent research has suggested using digestible P to assess availability, and due to nutritional interactions, there is also increasing interest in determining calcium (Ca) digestibility. The objectives of this study were to determine the standardized ileal digestibility (SID) of Ca and P in the feed ingredients for broilers. On day 18 post-hatch, 512 male and female broilers were individually weighed and randomly allocated to eight treatments with four replicates for each sex (eight birds/cage) in a randomized complete block design based on body weight and sex. No significant interaction between experimental diets and sex regarding the SID of Ca or P was found, and no effect of sex on the SID was observed. There were significant differences in the SID of the feed ingredients.Abstract This study aimed to determine the standardized ileal digestibility (SID) of calcium (Ca) and phosphorus (P) in various feed ingredients using the direct method. This study comprised eight experimental diets: a Ca-P-free diet and seven experimental diets, each containing monocalcium phosphate (MCP), dicalcium phosphate (DCP), monosodium phosphate (MSP) + limestone, corn, and soybean meal (SBM) as the sole sources of Ca and/or P. These diets provided 4.21 g/kg of non-phytate P from MCP, DCP, or MSP, and the MSP + limestone diet included 7.50 g/kg of Ca. The corn and SBM diets formulated to determine P digestibility maintained a dietary Ca/total P ratio of 1.4 through the addition of limestone. Chromic oxide was added to the diets as an indigestible index. On day 18, 256 male and 256 female broilers were individually weighed and randomly assigned to eight treatments, each with four replicates for each sex (eight birds per cage). This allocation followed a randomized complete block design based on body weight. On day 21, the birds were euthanized using carbon dioxide, and ileal digesta samples were collected from the distal two-thirds section of the ileum. No significant interactions between the experimental diets and sex regarding the SID of Ca or P were detected, and no effect of sex on the SID was observed. The standardized ileal Ca digestibility of MCP, DCP, limestone, corn, and SBM was found to be 84.7%, 70.1%, 52.6%, 88.6%, and 81.6%, respectively. The standardized ileal P digestibility of MCP, DCP, MSP, corn, and SBM was determined to be 91.8%, 76.8%, 94.4%, 73.1, and 88.4%, respectively. Given the variable digestibility of Ca and P across different feed ingredients, the consideration of the specific type of ingredients used in diet formulation is crucial. | Lee, Chae-Won; Kong, Changsu | Kyungpook Natl Univ, Dept Anim Sci & Biotechnol, Sangju 37224, South Korea; Kyungpook Natl Univ, Dept Anim Sci, Sangju 37224, South Korea; Kyungpook Natl Univ, Res Inst Innovat Anim Sci, Sangju 37224, South Korea | 59873229500; 36027521600 | chaewon2991@gmail.com;changsukong@knu.ac.kr; | ANIMALS | ANIMALS-BASEL | 2076-2615 | 14 | 17 | SCIE | AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES | 2024 | 2.7 | 12.1 | 2.16 | 2025-05-07 | 2 | 2 | broiler; calcium; feed ingredient; ileal digestibility; phosphorus | AMINO-ACID DIGESTIBILITY; SOYBEAN-MEAL; PERFORMANCE; RETENTION; METABOLISM; CHICKENS; APPARENT; POULTRY; MAIZE | broiler; calcium; feed ingredient; ileal digestibility; phosphorus | calcium; calcium phosphate dibasic; cobalamin; folic acid; limestone; lipopolysaccharide; phosphorus; phytate; pyridoxine; riboflavin; selenium; trace element; animal experiment; animal model; Article; chemical analysis; controlled study; diet composition; female; male; nonhuman; sampling; soybean meal; statistical analysis | English | 2024 | 2024-09 | 10.3390/ani14172603 | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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