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WoS SCOPUS Document Type Document Title Abstract Authors Affiliation ResearcherID (WoS) AuthorsID (SCOPUS) Author Email(s) Journal Name JCR Abbreviation ISSN eISSN Volume Issue WoS Edition WoS Category JCR Year IF JCR (%) FWCI FWCI Update Date WoS Citation SCOPUS Citation Keywords (WoS) KeywordsPlus (WoS) Keywords (SCOPUS) KeywordsPlus (SCOPUS) Language Publication Stage Publication Year Publication Date DOI JCR Link DOI Link WOS Link SCOPUS Link
Article Increased accuracy of estrus prediction using ruminoreticular biocapsule sensors in Hanwoo (Bos taurus coreanae) cows Visual estrus observation can only be confirmed at a rate of 50%-60%, which is lower than that obtained using a biosensor. Thus, the use of biosensors provides more opportunities for artificial insemination because it is easier to confirm estrus than by visual observation. This study determines the accuracy of estrus prediction using a ruminoreticular biosensor by analyzing ruminoreticular temperature during the estrus cycle and measuring changes in body activity. One hundred and twenty-five Hanwoo cows (64 with a ruminal biosensor in the test group and 61 without biosensors in the control group) were studied. Ruminoreticular temperatures and body activities were measured every 10 min. The first service of artificial insemination used gonadotropin-releasing hormone (GnRH)-based fixed-time artificial insemination protocol in the control and test groups. The test group received artificial insemination based on the estrus prediction made by the biosensor, and the control group received artificial insemination according to visual estrus observation. Before artificial insemination, the ruminoreticular temperature was maintained at an average of 38.95 +/- 0.05 degrees C for 13 h (-21 to -9 h), 0.73 degrees C higher than the average temperature observed at -48 h (38.22 +/- 0.06 degrees C). The body activity, measured using an indwelling 3-axis accelerometer, averaged 1502.57 +/- 27.35 for approximately 21 h from -4 to -24 h before artificial insemination, showing 203 indexes higher body activity than -48 hours (1299 +/- 9.72). Therefore, using an information and communication techonology (ICT)-based biosensor is highly effective because it can reduce the reproductive cost of a farm by accurately detecting estrus and increasing the rate of estrus confirmation in cattle. Kim, Daehyun; Kwon, Woo-Sung; Ha, Jaejung; Moon, Joonho; Yi, Junkoo Livestock Res Inst, Yeongju 36052, South Korea; Kyungpook Natl Univ, Dept Anim Sci & Biotechnol, Sangju 37224, South Korea; GenNBio Co Ltd, Pyeongtaek 17792, South Korea Kwon, Woo-Sung/J-6731-2019; Moon, Joonho/KFQ-2464-2024; Kwon, Woo-Sung/J-4391-2014; Yi, Junkoo/JBR-8507-2023 57211228843; 54383715800; 55848402300; 56410309500; 56182537200 kuma618@gmail.com;79lee38@korea.kr; JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY J ANIM SCI TECHNOL 2672-0191 2055-0391 65 4 SCIE AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES 2023 2.7 9.3 0.45 2025-06-25 1 2 Ruminoreticular biocapsule sensor; Ruminoreticular temperature; Body activity; Estrus detection; Conception rate; Hanwoo ARTIFICIAL-INSEMINATION; VAGINAL TEMPERATURE; DAIRY-COWS; TIME; SYNCHRONIZATION; OVULATION Body activity; Conception rate; Estrus detection; Hanwoo; Ruminoreticular biocapsule sensor; Ruminoreticular temperature English 2023 2023 10.5187/jast.2022.e125 바로가기 바로가기 바로가기 바로가기
Article Intestinal Immune Cell Populations, Barrier Function, and Microbiomes in Broilers Fed a Diet Supplemented with Chlorella vulgaris Simple Summary Microalgae have a broad nutritional composition of proteins, lipids, and bioactive compounds such as polysaccharides, carotenoids, and chlorophyll. Even at low inclusion levels, Chlorella vulgaris (CV) is a promising sustainable feed additive because of its positive effects on the nutritional and functional properties of broiler chickens. These features may play a vital role in the early stage of birds as intestinal and immune maturation can lead to improved gut development and production in later stages. Therefore, in this study, we investigated whether incorporating CV into the diet of 10-day-old broilers would enhance their growth performance, gut functional characteristics, and bacterial communities. This study aimed to evaluate the effects of dietary Chlorella vulgaris (CV) on the distribution of immune cells, intestinal morphology, intestinal barrier function, antioxidant markers, and the cecal microbiome in 10-day-old broiler chickens. A total of 120 day-old Ross 308 male broiler chicks were assigned to two dietary treatments using a randomized complete block design, with body weight as the blocking factor. Birds fed a diet containing CV showed an increase in CD4(+) T cells (p < 0.05) compared to those fed the control diet. The relative mRNA expression of intestinal epithelial barrier function-related markers (occludin and avian & beta;-defensin 5) was elevated (p < 0.05) in the CV-supplemented group compared to the control group. The alpha diversity indices (Chao1 and observed features) of the cecal microbiome in 10-day-old birds increased (p < 0.05), indicating higher richness within the cecal bacterial community. In the microbiome analysis, enriched genera abundance of Clostridium ASF356 and Coriobacteriaceae CHKCI002 was observed in birds fed the diet containing CV compared to those fed the control diet. Taken together, dietary CV supplementation might alter intestinal barrier function, immunity, and microbiomes in 10-day-old broiler chickens. Lee, Ji Young; Yoon, June Hyeok; An, Su Hyun; Cho, In Ho; Lee, Chae Won; Jeon, Yun Ji; Joo, Sang Seok; Ban, Byeong Cheol; Lee, Jae-Yeong; Jung, Hyun Jung; Kim, Minji; Kim, Z-Hun; Jung, Ji Young; Kim, Myunghoo; Kong, Changsu Pusan Natl Univ, Coll Nat Resources & Life Sci, Dept Anim Sci, Miryang 50463, South Korea; Kyungpook Natl Univ, Dept Anim Sci & Biotechnol, Sangju 37224, South Korea; Natl Inst Anim Sci, Rural Dev Adm, Anim Genet Resources Res Ctr, Wonju 55365, South Korea; Natl Inst Anim Sci, Anim Nutr & Physiol Team, Rural Dev Adm, Wonju 55365, South Korea; Nakdonggang Natl Inst Biol Resources NNIBR, Microbial Res Dept, Sangju 37242, South Korea; Pusan Natl Univ, Life & Ind Convergence Res Inst, Miryang 50463, South Korea; Kyungpook Natl Univ, Dept Anim Sci, Sangju 37224, South Korea; Kyungpook Natl Univ, Res Inst Horse Ind, Sangju 37224, South Korea; Hu Evergreen Pharm Inc, Incheon 21447, South Korea Kim, Seong-Hun/ABF-2927-2020; 안, 수현/JFK-5079-2023 58161969200; 57995779900; 57216974969; 58509432600; 59873229500; 58509922100; 57215860725; 57995927600; 57219980047; 24401615700; 57212315601; 36003195100; 58915180600; 36611749600; 36027521600 wldud15873@naver.com;junehyeokyoon@gmail.com;woobi89@gmail.com;inhoblog970630@gmail.com;chaewon2991@gmail.com;cecil0706@naver.com;ssjoo7680@gmail.com;banbyeongcheol@gmail.com;jay0i@korea.kr;hyjjung@korea.kr;mjkim00@korea.kr;kimzhun@gmail.com;jyjung@nnibr.re.kr;mhkim18@pusan.ac.kr;changsukong@knu.ac.kr; ANIMALS ANIMALS-BASEL 2076-2615 13 14 SCIE AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES 2023 2.7 9.3 3.69 2025-06-25 10 12 microalgae; immune cells; gut health; microbiome; broilers GROWTH-PERFORMANCE; MEAT QUALITY; MICROALGAE; MORPHOLOGY; ACID; MICROFLORA; CHICKENS; INDEXES; STRESS broilers; gut health; immune cells; microalgae; microbiome arginine; beta defensin 5; catalase; CD3 antigen; CD4 antigen; citric acid; colecalciferol; copper; cysteine; edetic acid; ferric ammonium citrate; folic acid; glutathione peroxidase; histidine; isoleucine; malonaldehyde; manganese; methionine; mucin 2; nicotinamide; nitric oxide; occludin; pantothenate calcium; pyridoxine; retinol acetate; riboflavin; RNA 16S; selenium; superoxide dismutase; threonine; valine; animal experiment; antigen presenting cell; Article; Bacteroides; Bacteroidetes; broiler; CD4+ T lymphocyte; CD8+ T lymphocyte; Chlorella vulgaris; Clostridium; Coriobacteriaceae; Coriobacteriales; diet supplementation; discriminant analysis; Eggerthellaceae; Firmicutes; flow cytometry; food intake; gas chromatography; gene expression; immunocompetent cell; intestine flora; jugular vein; Lachnospiraceae; lamina propria; macrophage; male; microalga; microbial community; microbiome; neutrophil; nonhuman; Proteobacteria; real time polymerase chain reaction; RNA extraction English 2023 2023-07 10.3390/ani13142380 바로가기 바로가기 바로가기 바로가기
Article Lauric acid reduces apoptosis by inhibiting FOXO3a-signaling in Deoxynivalenol-treated IPEC-J2 cells Deoxynivalenol (DON) is the most common mycotoxin contaminant of food or feed worldwide and causes disease in animals. Lauric acid (LA) is a medium-chain fatty acid useful for barrier functions such as antimicrobial activity in the intestine of monogastric animals. However, the molecular mechanisms by which lauric acid exerts its effects on the deoxynivalenol-exposed small intestine have not been studied. We used an intestinal porcine epithelial cell line (IPEC-J2) as an in vitro model to explore the molecular mechanism of lauric acid in alleviating deoxynivalenol-induced damage. We found that lauric acid reversed deoxynivalenol-induced reduction in cell viability. Our qRT-PCR results indicated that lauric acid alleviated deoxynivalenol-induced apoptosis through Annexin-V. Additionally, immunofluorescence and Western blotting showed that lauric acid attenuated deoxynivalenol-induced Forkhead box O3 (FOXO3a) translocation. These results suggest that lauric acid attenuates Forkhead box O3 translocation in the small intestine damaged by deoxynivalenol, thereby reducing apoptosis. In conclusion, this study found that lauric acid alleviates deoxynivalenol-induced damage in intestinal porcine epithelial cell line through various molecular mechanisms. © 2023 Korean Society of Animal Sciences and Technology. All rights reserved. Kim, Na Yeon; In Lee, Sang Department of Animal Biotechnology, Kyungpook National University, Sangju, Gyeongsangbuk-do, 37224, South Korea; Department of Animal Biotechnology, Kyungpook National University, Sangju, Gyeongsangbuk-do, 37224, South Korea 59884547500; 57203597336 silee78@knu.ac.kr; Journal of Animal Science and Technology J ANIM SCI TECHNOL 2672-0191 2055-0391 SCIE AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES 2023 2.7 9.3 0 2025-06-25 0 Apoptosis; Deoxynivalenol; Foxo3a; IPEC-J2 cells; Lauric acid English Final 2023 10.5187/jast.2023.e92 바로가기 바로가기 바로가기
Article Molecular Detection of Porcine Parainfluenza Viruses 1 and 5 Using a Newly Developed Duplex Real-Time RT-PCR in South Korea Simple Summary In the present study, we first developed a highly specific and sensitive TaqMan probe-based duplex real-time reverse transcription polymerase chain reaction (dqRT-PCR) assay for simultaneous and differential detection of porcine parainfluenza virus 1 (PPIV1) and PPIV5 in a single reaction. The developed dqRT-PCR, with high sensitivity, specificity, and accuracy, was useful for the detection of PPIV1 and PPIV5 in clinical pig samples, and the diagnostic sensitivity was higher than that of the previous qRT-PCR for PPIV1 and consistent with that of the previous qRT-PCR for PPIV5. Using the dqRT-PCR, we demonstrated that PPIV1 and PPIV5 are co-circulating in Korean pig herds, and subsequent phylogenetic analysis suggested that genetically diverse PPIV1 strains are presented in Korean pig herds. The new dqRT-PCR developed in this study will be a valuable tool for diagnosis and further studies for PPIV1 and PPIV5. Two species of porcine parainfluenza viruses (PPIV), PPIV1 and PPIV5, are globally distributed in pig herds and associated with porcine respiratory diseases, and a diagnostic tool for the simultaneous detection of the two viruses is required. In this study, a TaqMan probe-based duplex real-time reverse transcription polymerase chain reaction (dqRT-PCR) assay was first developed for the differential detection of PPIV1 and PPIV5 nucleocapsid protein (NP) genes in porcine clinical samples. The dqRT-PCR assay was highly sensitive, its limit of detection was approximately 10 RNA copies/reaction, it specifically amplified the targeted NP genes of PPIV1 and PPIV5 without cross-reacting with other porcine pathogens, and their clinical detection rates were 15.2% and 0.7%, respectively. The results from 441 clinical samples taken from 278 Korean domestic pig farms showed that the prevalence of PPIV1 and PPIV5 was 11.2% and 1.1%, respectively, and co-infection of both viruses was confirmed in a farm, suggesting that PPIV1 and PPIV5 are co-circulating in current Korean pig herds. Phylogenetic analysis based on the partial NP genes suggested that genetically diverse PPIV1 strains are circulating in Korean pig herds. The developed dqRT-PCR assay was found to be an accurate, reliable, and quantitative detection tool for PPIV1 and PPIV5 RNA in clinical pig samples and will be useful for etiological and epidemiological studies and the control of viral infections in the field. Kim, Jong-Min; Kim, Hye-Ryung; Jeon, Gyu-Tae; Baek, Ji-Su; Kwon, Oh-Deog; Park, Choi-Kyu Kyungpook Natl Univ, Coll Vet Med, Anim Dis Intervent Ctr, Daegu 41566, South Korea 57277964700; 57195772612; 59366422400; 58111837700; 7402195886; 24768064900 parkck@knu.ac.kr; ANIMALS ANIMALS-BASEL 2076-2615 13 4 SCIE AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES 2023 2.7 9.3 2.35 2025-06-25 8 7 porcine parainfluenza virus 1; porcine parainfluenza virus 5; dqRT-PCR COMPLETE GENOME SEQUENCE; IN-VITRO; IDENTIFICATION; PARAMYXOVIRUS; GUIDELINES; ISOLATE; CANINE; PIGS dqRT-PCR; porcine parainfluenza virus 1; porcine parainfluenza virus 5 animal experiment; Article; domestic pig; gene expression; gene sequence; genetic variability; Human parainfluenza virus 1; molecular diagnosis; nonhuman; Parainfluenza virus 5; phylogeny; prevalence; real time reverse transcription polymerase chain reaction; sensitivity and specificity; virus nucleocapsid English 2023 2023-02 10.3390/ani13040598 바로가기 바로가기 바로가기 바로가기
Article Rate-induced tipping in complex high-dimensional ecological networks In an ecosystem, environmental changes as a result of natural and human processes can cause some key parameters of the system to change with time. Depending on how fast such a parameter changes, a tipping point can occur. Existing works on rate-induced tipping, or R-tipping, offered a theoretical way to study this phenomenon but from a local dynamical point of view, revealing, e.g., the existence of a critical rate for some specific initial condition above which a tipping point will occur. As ecosystems are subject to constant disturbances and can drift away from their equilibrium point, it is necessary to study R-tipping from a global perspective in terms of the initial conditions in the entire relevant phase space region. In particular, we introduce the notion of the probability of R-tipping defined for initial conditions taken from the whole relevant phase space. Using a number of real-world, complex mutualistic networks as a paradigm, we find a scaling law between this probability and the rate of parameter change and provide a geometric theory to explain the law. The real-world implication is that even a slow parameter change can lead to a system collapse with catastrophic consequences. In fact, to mitigate the environmental changes by merely slowing down the parameter drift may not always be effective: Only when the rate of parameter change is reduced to practically zero would the tipping be avoided. Our global dynamics approach offers a more complete and physically meaningful way to understand the important phenomenon of R-tipping. Panahi, Shirin; Do, Younghae; Hastings, Alan; Lai, Ying-Cheng Arizona State Univ, Sch Elect Comp & Energy Engn, Tempe, AZ 85287 USA; Kyungpook Natl Univ, Nonlinear Dynam Math Applicat Ctr, Dept Math, Daegu 41566, South Korea; Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA; Santa Fe Inst, Santa Fe, NM 87501 USA; Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA ; Hastings, Alan/O-6864-2019; Do, Younghae/G-3522-2011 57220564996; 7103101109; 7102581412; 7401512359 Ying-Cheng.Lai@asu.edu;YingCheng.Lai@asu.edu; PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA P NATL ACAD SCI USA 0027-8424 1091-6490 120 51 SCIE MULTIDISCIPLINARY SCIENCES 2023 9.4 9.3 0.94 2025-06-25 9 9 tipping point; rate-induced tipping; mutualistic networks; nonlinear dynamics; scaling law CRITICAL SLOWING-DOWN; ANIMAL MUTUALISTIC NETWORKS; EARLY-WARNING SIGNALS; POINTS; SYSTEMS; ARCHITECTURE; COEVOLUTION; TRANSITIONS; EXTINCTION; RESILIENCE mutualistic networks; nonlinear dynamics; rate-induced tipping; scaling law Article; climate change; computer analysis; coral reef; dynamics; ecosystem; environmental change; environmental factor; growth rate; mathematical model; mathematical parameters; nonlinear system; probability; rate induced tipping; time series analysis; adolescent; article; ecosystem; human; law; nonlinear system English 2023 2023-12-19 10.1073/pnas.2308820120 바로가기 바로가기 바로가기 바로가기
Article Regular medical checkup program (in K-MEDI hub) to enhance the welfare of laboratory dogs and pigs BackgroundThe importance of animal welfare is being recognized worldwide. Recently, the increasing demand for enhanced laboratory animal welfare has led to clinically featured transformations of animal research institutes. This study aims to describe the process and findings of veterinary medical check-ups and its influence on laboratory dogs and pigs welfare. Regular medical checkups were conducted by the attending veterinarian twice a year to ensure the health and welfare of dogs and pigs in our animal research institute. Based on the findings from the medical checkup, we assessed the current health of dogs and pigs,providing reasonable treatments to prevent the risk of complications.ResultsBlood tests and physical examinations revealed clinically relevant findings. Some of these findings were due to insufficient postoperative care after invasive surgical experiments and the remaining were predictable side effects after surgical experiments. However, one finding involved severe gum bleeding due to retained deciduous teeth. This animal was euthanized because it was judged to reach the humane endpoint. Majority of the dogs and pigs at our animal research institute were considered to be healthy, based on the comprehensive results of the medical checkups.ConclusionsRegular medical checkups by the attending veterinarian established enhanced animal welfare, ensuring the accuracy and reproducibility of animal studies. This pioneering veterinary animal care program can serve as a potential advanced guideline for animal research institutes to improve dogs and pigs welfare. Lee, Gwang-Hoon; Jo, Woori; Park, Joon-Suk; Kang, Tae-Ku; Sung, Soo-Eun; Oh, Taeho; Kim, KilSoo Daegu Gyeongbuk Med Innovat Fdn, Preclin Res Ctr, Daegu 41061, South Korea; Kyungpook Natl Univ, Coll Vet Med, Dept Vet Internal Med, Daegu 41566, South Korea; Kyungpook Natl Univ, Coll Vet Med, Dept Vet Toxicol, Daegu 41566, South Korea Lee, GwangHoon/KHU-9790-2024 57266540000; 55848825000; 57674027800; 57218871402; 56051194000; 8251199600; 35272034300 thoh@knu.ac.kr;kskim728@knu.ac.kr; LABORATORY ANIMAL RESEARCH LAB ANIM RES 1738-6055 2233-7660 39 1 ESCI MEDICINE, RESEARCH & EXPERIMENTAL;VETERINARY SCIENCES 2023 2.7 9.3 0.31 2025-06-25 3 3 Attending Veterinarian; Laboratory animal; Dog; Pig; Animal welfare; Medical checks ANIMAL-WELFARE; OVERWEIGHT; RISK Animal welfare; Attending Veterinarian; Dog; Laboratory animal; Medical checks; Pig English 2023 2023-10-24 10.1186/s42826-023-00170-7 바로가기 바로가기 바로가기 바로가기
Article Stress Evaluation of Mouse Husbandry Environments for Improving Laboratory Animal Welfare Simple Summary It is well recognized that companionship is important to animals and that they need to be provided with an environment accompanied by materials for enrichment, such as toys. However, few studies have evaluated whether specific environments actually benefit animals. Therefore, we designed various environments for laboratory animals and scientifically evaluated which environments reduced these animals' stress. We found that an environment with freer air circulation and the provision of enrichment materials reduced animal stress, and no risk or benefit could be determined for the presence or absence of a companion. We do not consider that our results necessarily indicate the lack of a need for a companion, but, rather, the importance of having a good companion. Our results can serve as a meaningful guideline for the creation of suitable environments for laboratory animals. Animal welfare is recognized as essential for the coexistence of humans and animals. Considering the increased demand and interest in animal welfare, many methods for improving animal welfare are being devised, but which method reduces animal stress has not been scientifically verified. Therefore, reducing animal stress by providing a proper breeding environment and environmental enrichment can be the basis for animal study. In this study, stress levels were assessed based on the mouse-breeding environment. We considered that the higher the body weight and the lower the corticosterone concentration, the lower the stress. According to the results, animals in the individual ventilation cages were determined to have lower serum cortisol concentrations, while the body weight of the animals was increased when in individual ventilation cages compared with individual isolated cages and when providing environmental enrichment compared with group breeding or not providing environmental enrichment. The results provide appropriate guidelines for improving laboratory animal welfare. Lee, Gwang-Hoon; Kim, KilSoo; Jo, Woori Daegu Gyeongbuk Med Innovat Fdn, Preclin Res Ctr, Daegu 41061, South Korea; Kyungpook Natl Univ, Coll Vet Med, Dept Vet Toxicol, Daegu 41566, South Korea Lee, GwangHoon/KHU-9790-2024 57266540000; 35272034300; 55848825000 kskim728@knu.ac.kr;c2dar@kmedihub.re.kr; ANIMALS ANIMALS-BASEL 2076-2615 13 2 SCIE AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES 2023 2.7 9.3 3.36 2025-06-25 9 10 animal welfare; environmental enrichment; housing; laboratory animals; stress evaluation BODY-WEIGHT; ENRICHMENT; MICE; CORTICOSTERONE; POTENTIATION; AGGRESSION; ANXIETY animal welfare; environmental enrichment; housing; laboratory animals; stress evaluation corticosterone; hydrocortisone; isoflurane; animal experiment; animal housing; animal husbandry; animal welfare; Article; artificial ventilation; bloodletting; body weight; breeding; environment; environmental enrichment; experimental animal welfare; food intake; hydrocortisone blood level; male; mouse; nonhuman; physiological stress; practice guideline English 2023 2023-01 10.3390/ani13020249 바로가기 바로가기 바로가기 바로가기
Article T-cell lymphoma infiltrating the uterus and ovaries of a Golden Retriever: a case report BackgroundTo the best of our knowledge, this is the first report of female genital system infiltration of T-cell lymphoma in veterinary literature.Case presentationA 1.5-year-old, intact female Golden Retriever was referred due to melena and hyporexia that lasted for three weeks. Fever (40.5celcius), tachycardia, tachypnoea, pale mucous membranes, and purulent vaginal discharge were identified on physical examination. Blood analyses revealed leucocytosis, anaemia, hypoalbuminemia, and increased lactate and C-reactive protein levels. On abdominal radiography, the small intestine was moderately deviated because of an oval-shaped mass (13 cm x 8.7 cm) located in the mid-abdomen. An enlarged tubular-shaped structure that had the opacity of soft tissue located in dorsal to the bladder to the middle of the abdomen, and an oval-shaped mass (5.28 cm x 3.26 cm), which was suspected to be a medial iliac lymph node located at the sixth to seventh lumbar level. Abdominal ultrasonography revealed gas and fluid in the lumen of the uterine horn with a severely thickened wall, round enlarged lymph nodes around the genitourinary system, and free fluid in the abdominal cavity. Based on these results, pyometra was suspected, and an exploratory laparotomy was performed for ovariohysterectomy. The resected ovary and uterus were macroscopically hypertrophied. Histopathological examination of the ovary and uterus revealed neoplastic proliferation of large round cells with strong immunoreactivity for CD3, indicating T-cell lymphoma. Therefore, the young dog was diagnosed with genital lymphoma.ConclusionsThe present report describes T-cell lymphoma infiltrating the uterus and ovaries in a young dog, which is rarely diagnosed and could aid in the differential diagnosis of genital diseases in young dogs. Jo, Jaeyeop; Son, Mingyun; Chae, Yeon; Yun, Taesik; Koo, Yoonhoi; Lee, Dohee; Kang, Hyun-Gu; Kang, Byeong-Teck; Yang, Mhan-Pyo; Kim, Hakhyun Chungbuk Natl Univ, Coll Vet Med, Lab Vet Internal Med, Cheongju 28644, Chungbuk, South Korea; Kyungpook Natl Univ, Coll Vet Med, Daegu 41566, South Korea; Chungbuk Natl Univ, Coll Vet Med, Lab Vet Obstet, Cheongju 28644, South Korea 58572623800; 57490438300; 57215577118; 57209458067; 57220050745; 57226189094; 55194469400; 8858992400; 7404927049; 57192503045 kimh@chungbuk.ac.kr; IRISH VETERINARY JOURNAL IRISH VET J 0368-0762 2046-0481 76 1 SCIE VETERINARY SCIENCES 2023 2.7 9.3 0.37 2025-06-25 1 1 Canine; Genital disease; Lymphoma; Ovariohysterectomy; Pyometra CANINE; TUMORS; CLASSIFICATION; INFLAMMATION; NEOPLASMS; CANCER; SEPSIS; DOGS Canine; Genital disease; Lymphoma; Ovariohysterectomy; Pyometra 6 n,n' dimethylarginine; alanine aminotransferase; alkaline phosphatase; aspartate aminotransferase; bile acid; butorphanol; C reactive protein; chloride; creatinine; enrofloxacin; gamma glutamyltransferase; globulin; glucose; lactate dehydrogenase; lactic acid; maropitant; phosphorus; potassium; sodium; sultamicillin; transcription factor PAX5; triacylglycerol; abdominal pain; anemia; animal experiment; animal model; animal tissue; Article; Canine distemper virus; cell infiltration; diffuse large B cell lymphoma; electrolyte blood level; female; female genital system; fever; golden retriever; histopathology; immunoreactivity; nonhuman; ovary; pleura effusion; polyuria; systemic inflammatory response syndrome; T cell lymphoma; tachycardia; tachypnea; thoracocentesis; uterus English 2023 2023-09-13 10.1186/s13620-023-00252-x 바로가기 바로가기 바로가기 바로가기
Article Tailored Multiplex Real-Time RT-PCR with Species-Specific Internal Positive Controls for Detecting SARS-CoV-2 in Canine and Feline Clinical Samples Simple Summary Given that SARS-CoV-2 infections in companion dogs and cats have been frequently reported worldwide during the ongoing COVID-19 pandemic, a multiplex real-time RT-PCR assay is urgently required to reliably detect SARS-CoV-2 infection in companion animals. In this study, we developed a tailored multiplex real-time RT-PCR assay to simultaneously detect RdRp and N genes of currently circulating SARS-CoV-2 variants and canine or feline 16S rRNA as an endogenous internal positive control. The developed assay had high sensitivity, specificity, and accuracy and could detect all tested SARS-CoV-2 variants, including Omicron subvariants. Clinical evaluation of canine and feline specimens revealed that the diagnostic sensitivity of the assay was equivalent to that of a commercial SARS-CoV-2 multiplex real-time RT-PCR kit. Furthermore, canine or feline endogenous internal positive control was amplified using the developed assay while avoiding false-negative results. Considering the high sensitivity, specificity, accuracy, and reliability, the developed assay can help diagnose COVID-19 in dogs and cats and potentially play a vital role in the rapid diagnosis and control of SARS-CoV-2 infections in companion animals. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections have been frequently reported in companion dogs and cats worldwide during the ongoing coronavirus disease. However, RT-qPCR methods developed for humans have been used for the diagnosis of SARS-CoV-2 infections in suspected companion dogs and cats owing to the lack of the companion animal-tailored methods. Therefore, we developed a multiplex RT-qPCR (mRT-qPCR) using newly designed primers and probes targeting RdRp and N genes of all currently circulating SARS-CoV-2 variants as well as the canine or feline 16S rRNA gene as an endogenous internal positive control (EIPC) for reliable diagnosis of SARS-CoV-2 infection from suspected dogs and cats. The developed mRT-qPCR assay specifically detected the target genes of SARS-CoV-2 but no other canine or feline pathogens. Furthermore, canine and feline EIPCs were stably amplified by mRT-qPCR in samples containing canine- or feline-origin cellular materials. This assay has high repeatability and reproducibility, with an optimal limit of detection (<10 RNA copies per reaction) and coefficients of variation (<1.0%). The detection rate of SARS-CoV-2 of the developed mRT-qPCR was 6.6% for canine and feline nasopharyngeal samples, which was consistent with that of a commercial mRT-qPCR kit for humans. Collectively, the newly developed mRT-qPCR with canine and feline EIPC can efficiently diagnose and evaluate the viral load in field specimens and will be a valuable tool for etiological diagnosis, epidemiological study, and controlling SARS-CoV-2 infections in canine and feline populations. Jeon, Gyu-Tae; Kim, Hye-Ryung; Kim, Jong-Min; Baek, Ji-Su; Shin, Yeun-Kyung; Kwon, Oh-Kyu; Kang, Hae-Eun; Cho, Ho-Seong; Cheon, Doo-Sung; Park, Choi-Kyu Kyungpook Natl Univ, Coll Vet Med, Anim Dis Intervent Ctr, Daegu 41566, South Korea; Anim & Plant Quarantine Agcy, Foreign Anim Dis Div, Gimcheon 39660, South Korea; Jeonbuk Natl Univ, Biosafety Res Inst, Coll Vet Med, Iksan 54596, South Korea; Postbio Inc, Guri Si 11906, South Korea CHO, Ho-Seong/K-8386-2015; Cho, Ho-Seong/K-8386-2015 59366422400; 57195772612; 57277964700; 58111837700; 7402816546; 58077549200; 7404071354; 12765051200; 6603797758; 24768064900 parkck@knu.ac.kr; ANIMALS ANIMALS-BASEL 2076-2615 13 4 SCIE AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES 2023 2.7 9.3 0.67 2025-06-25 2 2 SARS-CoV-2; multiplex real-time RT-PCR; RdRp gene; N gene; internal positive control VARIANTS; IMPACT; RNA internal positive control; multiplex real-time RT-PCR; N gene; RdRp gene; SARS-CoV-2 RNA 16S; RNA directed RNA polymerase; virus RNA; Article; Bordetella bronchiseptica; canine distemper; Canine distemper virus; Canis; cat; controlled study; CRFK cell line; Feline herpesvirus; limit of detection; MDCK cell line; measurement precision; measurement repeatability; multiplex real time polymerase chain reaction; nasopharyngeal swab; nonhuman; Parainfluenza virus infection; Porcine reproductive and respiratory syndrome virus; reproducibility; RNA extraction; sensitivity and specificity; sequence alignment; Severe acute respiratory syndrome coronavirus 2; spectrophotometry; Vero cell line; virus detection English 2023 2023-02 10.3390/ani13040602 바로가기 바로가기 바로가기 바로가기
Article Upregulation of the ERRγ-VDAC1 axis underlies the molecular pathogenesis of pancreatitis Emerging evidence suggest that transcription factors play multiple roles in the development of pancreatitis, a necroinflammatory condition lacking specific therapy. Estrogen-related receptor ? (ERR?), a pleiotropic transcription factor, has been reported to play a vital role in pancreatic acinar cell (PAC) homeostasis. However, the role of ERR? in PAC dysfunction remains hitherto unknown. Here, we demonstrated in both mice models and human cohorts that pancreatitis is associated with an increase in ERR? gene expression via activation of STAT3. Acinar-specific ERR? haploinsufficiency or pharmacological inhibition of ERR? significantly impaired the progression of pancreatitis both in vitro and in vivo. Using systematic transcriptomic analysis, we identified that voltage-dependent anion channel 1 (VDAC1) acts as a molecular mediator of ERR?. Mechanistically, we showed that induction of ERR? in cultured acinar cells and mouse pancreata enhanced VDAC1 expression by directly binding to specific site of the Vdac1 gene promoter and resulted in VDAC1 oligomerization. Notably, VDAC1, whose expression and oligomerization were dependent on ERR?, modulates mitochondrial Ca2+ and ROS levels. Inhibition of the ERR?-VDAC1 axis could alleviate mitochondrial Ca2+ accumulation, ROS formation and inhibit progression of pancreatitis. Using two different mouse models of pancreatitis, we showed that pharmacological blockade of ERR?-VDAC1 pathway has therapeutic benefits in mitigating progression of pancreatitis. Likewise, using PRSS1R122H-Tg mice to mimic human hereditary pancreatitis, we demonstrated that ERR? inhibitor also alleviated pancreatitis. Our findings highlight the importance of ERR? in pancreatitis progression and suggests its therapeutic intervention for prevention and treatment of pancreatitis. Chanda, Dipanjan; Thoudam, Themis; Sinam, Ibotombi Singh; Lim, Chae Won; Kim, Myeongjin; Wang, Jiale; Lee, Kyeong-Min; Ma, Jing; Saxena, Romil; Choi, Jinhyuk; Oh, Chang Joo; Lee, Hoyul; Jeon, Yong Hyun; Cho, Sung Jin; Jung, Hoe-Yune; Park, Keun-Gyu; Choi, Hueng-Sik; Suh, Jae Myoung; Auwerx, Johan; Ji, Baoan; Liangpunsakul, Suthat; Jeon, Jae-Han; Lee, In-Kyu Kyungpook Natl Univ, Res Inst Aging & Metab, Daegu 41404, South Korea; Kyungpook Natl Univ Hosp, Biomed Res Inst, Daegu 41404, South Korea; Mayo Clin, Dept Canc Biol, Jacksonville, FL 32066 USA; Daegu Gyeongbuk Inst Sci & Technol, Div Biotechnol, Daegu 42988, South Korea; Indiana Univ Sch Med, Dept Med, Div Gastroenterol & Hepatol, Indianapolis, IN 46202 USA; Indiana Univ Sch Med, Dept Pathol & Lab Med, Indianapolis, IN 46202 USA; Korea Adv Inst Sci & Technol, Grad Sch Med Sci & Engn, Daejeon 34141, South Korea; Daegu Gyeongbuk Med Innovat Fdn, Lab Anim Ctr, Daegu 41061, South Korea; Daegu Gyeongbuk Med Innovat Fdn, New Drug Dev Ctr, Daegu 41061, South Korea; R&D Ctr NovMetaPharma Co Ltd, Pohang 37688, South Korea; Pohang Univ Sci & Technol, Sch Interdisciplinary Biosci & Bioengn, Pohang 37673, South Korea; Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Dept Internal Med, Daegu 41944, South Korea; Chonnam Natl Univ, Sch Biol Sci & Technol, Gwangju 61186, South Korea; Ecole Polytech Fed Lausanne, Lab Integrat Syst Physiol, CH-1015 Lausanne, Switzerland; Kyungpook Natl Univ, Chilgok Hosp, Sch Med, Dept Internal Med, Daegu 41404, South Korea Jeon, Yong/N-6910-2019; Suh, Jae/B-3268-2015; Auwerx, Johan/ABE-9307-2021; Wang, Jiale/KWU-9590-2024; Lee, In-Kyu/AAR-6374-2021; thoudam, themis/ACM-3919-2022; Lee, YoungMi/JCF-0461-2023; Chanda, Dipanjan/AAU-3996-2021 16416525900; 57192905626; 57846985600; 58158274300; 58158274200; 57211340211; 35242750600; 57201898640; 7202190528; 59643638200; 14049080600; 58017533900; 16042453400; 58735369700; 35484149000; 57202558343; 7404338771; 36799636000; 57414044100; 7102566163; 6602240165; 36910340400; 59060573600 ggoloo@hanmail.net;leei@knu.ac.kr; PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA P NATL ACAD SCI USA 0027-8424 1091-6490 120 20 SCIE MULTIDISCIPLINARY SCIENCES 2023 9.4 9.3 1.18 2025-06-25 10 10 nuclear receptor; ERR & gamma;; VDAC1; mitochondrial Ca2+; pancreatitis ERR-GAMMA; INVERSE AGONIST; MOUSE MODEL; AUTOPHAGY; INFLAMMATION; HOMEOSTASIS; INHIBITION; EXPRESSION; ALCOHOL; ROLES Animals; Humans; Mice; Pancreatitis, Chronic; Reactive Oxygen Species; Up-Regulation; Voltage-Dependent Anion Channel 1; calcium ion; estrogen related receptor alpha; reactive oxygen metabolite; STAT3 protein; voltage dependent anion channel 1; reactive oxygen metabolite; VDAC1 protein, human; voltage dependent anion channel 1; animal experiment; animal model; Article; binding site; calcium metabolism; cell culture; cohort analysis; controlled study; disease exacerbation; gene expression; haploinsufficiency; in vitro study; in vivo study; male; mouse; nonhuman; oligomerization; pancreatitis; pathogenesis; promoter region; protein binding; protein expression; transcriptomics; upregulation; animal; chronic pancreatitis; human; metabolism; upregulation English 2023 2023-05-16 10.1073/pnas.2219644120 바로가기 바로가기 바로가기 바로가기
Article Clinical Features and Natural History of Paediatric Patients with Ulcerative Proctitis: A Multicentre Study from the Paediatric IBD Porto Group of ESPGHAN Background and Aims: Ulcerative proctitis [UP] is an uncommon presentation in paediatric patients with ulcerative colitis. We aimed to characterize the clinical features and natural history of UP in children, and to identify predictors of poor outcomes. Methods: This was a retrospective study involving 37 sites affiliated with the IBD Porto Group of ESPGHAN. Data were collected from patients aged = 35 providing an increased risk for poor outcomes. By the end of follow-up, 3.1% of patients underwent colectomy. Patients with UP that experienced proximal disease progression during follow-up [48%] had significantly higher rates of a caecal patch at diagnosis and higher PUCAI score by the end of induction, compared to those without progression. Conclusion: Paediatric patients with UP exhibit high rates of treatment escalation and proximal disease extension. Tal, Noa; Tzivinikos, Christos; Gasparetto, Marco; Serban, Daniela E.; Zifman, Eyal; Hojsak, Iva; Ledder, Oren; Feler, Anat Yerushalmy; Rolandsdotter, Helena; Aloi, Marina; Bramuzzo, Matteo; Buderus, Stephan; Lionetti, Paolo; Norsa, Lorenzo; Norden, Christoph; Urlep, Darja; Romano, Claudio; Shaoul, Ron; Martinez-Vinson, Christine; Karoliny, Anna; De Greef, Elisabeth; Kang, Ben; VIckova, Eva; Alvisi, Patrizia; Kori, Michal; Tavares, Marta; Weiss, Batia; Hussey, Seamus; Qamhawi, Maria E.; Perez, Laura M. Palomino; Henderson, Paul; Parmar, Raj; Miele, Erasmo; Rinawi, Firas; Lozano-Ruf, Ana; Zamvar, Veena; Kolho, Kaija-Leena; Shouval, Dror S. Schneider Childrens Med Ctr, Inst Gastroenterol Nutr & Liver Dis, Petah Tiqwa, Israel; Tel Aviv Univ, Fac Med, Tel Aviv, Israel; Mohammed Bin Rashid Univ, Al Jalila Childrens Specialty Hosp, Paediat Gastroenterol Dept, Dubai, U Arab Emirates; Queen Mary Univ London, Blizard Inst, Royal London Childrens Hosp, Dept Paediat Gastroenterol,Barts Hlth NHS Trust,C, London, England; Iuliu Hatieganu Univ Med & Pharm, Clin Pediat 2, Emergency Clin Hosp Children, Cluj Napoca, Romania; Meir Med Ctr, Pediat Gastroenterol Unit, Kefar Sava, Israel; Childrens Hosp Zagreb, Referral Ctr Pediat Gastroenterol & Nutr, Zagreb, Croatia; Hebrew Univ Jerusalem, Juliet Keidan Inst Pediat Gastroenterol & Nutr, Shaare Zedek Med Ctr, Jerusalem, Israel; Tel Aviv Sourasky Med Ctr, Pediat Gastroenterol Inst, Dana Dwek Childrens Hosp, Tel Aviv, Israel; Karolinska Inst, Dept Clin Sci & Educ, Sodersjukhuset, Stockholm, Sweden; Sachs Children & Youth Hosp, Dept Gastroenterol, Sodersjukhuset, Stockholm, Sweden; Sapienza Univ Rome, Pediat Gastroenterol Hepatol & Nutr Inst, Rome, Italy; IRCCS Burlo Garofolo, Institute Maternal & Child Hlth, Trieste, Italy; St Marien Hosp, Dept Pediat, Bonn, Germany; Univ Florence, Meyer Childrens Hosp, Dept NEUROFARBA, Florence, Italy; ASST Papa Giovanni XXIII, Pediat Hepatol Gastroenterol & Transplantat Unit, Bergamo, Italy; Hvidovre Univ Hosp, Dept Pediat, Copenhagen, Denmark; Univ Med Ctr Ljubljana, Univ Childrens Hosp, Pediat Gastroenterol & Liver Unit, Ljubljana, Slovenia; Univ Messina, Dept Human Pathol Adulthood & Childhood G Barresi, Pediat Gastroenterol & Cyst Fibrosis Unit, Messina, Italy; Ruth Childrens Hosp Haifa, Pediat Gastroenterol & Nutr Inst, Rambam Med Ctr, Fac Med, Haifa, Israel; Hop Univ Robert Debre, Serv Gastroenterol & Nutr Pediat, Paris, France; Heim Pal Natl Pediat Inst, Budapest, Hungary; Free Univ Brussels, Dept Paediat Gastroenterol & Nutr, Kidz Hlth Castle UZ Brussels, Brussels, Belgium; Kyungpook Natl Univ, Sch Med, Dept Pediat, Daegu, South Korea; Charles Univ Prague, Med Fac 2, Dept Pediat, Prague, Czech Republic; Univ Hosp Motol, Prague, Czech Republic; Maggiore Hosp, Pediat Gastroenterol Unit, Bologna, Italy; Kaplan Med Ctr, Pdiatr Gastroenterol, Rehovot, Israel; Hebrew Univ Jerusalem, Fac Med, Jerusalem, Israel; Ctr Hosp & Univ Porto, Ctr Materno Infantil Norte, Dept Pediat Gastroenterol, Porto, Portugal; Edmond & Lily Safra Childrens Hosp, Div Pediat Gastroenterol & Nutr, Ramat Gan, Israel; UCD & RCSI, Childrens Hlth Ireland, Dublin, Ireland; Karolinska Univ Hosp, Astrid Lindgren Childrens Hosp, Dept Gastroenterol Hepatol & Nutr, Stockholm, Sweden; Hosp Infantil Univ Nino Jesus, Gastroenterol & Nutr Dept, Madrid, Spain; Royal Hosp Children & Young People, Dept Paediat Gastroenterol & Nutr, Edinburgh, Midlothian, Scotland; Great North Childrens Hosp, Dept Pediat Gastroenterol, Newcastle, NSW, Australia; Univ Naples Federico II, Sect Pediat, Dept Translat Med Sci, Naples, Italy; Emek Med Ctr, Pediat Gastroenterol Unit, Afula, Israel; Technion Israel Inst Technol, Ruth & Bruce Rappaport Fac Med, Afula, Israel; Hosp St Joan de Deu, Dept Pediat Gastroenterol Hepatol & Nutr, Barcelona, Spain; Leeds Childrens Hosp, Leeds Teaching Hosp NHS Trust, Dept Paediat Gastroenterol, Leeds, W Yorkshire, England; Univ Helsinki, Childrens Hosp, Helsinki, Finland; Univ Tampere, Fac Med & Hlth Technol, Tampere, Finland Norsa, Lorenzo/I-1462-2018; Miele, Erasmo/A-1906-2010; Bramuzzo, Matteo/K-2504-2018; Serban, Daniela Elena/V-4326-2017; Aloi, Marina/K-2496-2016; Hojsak, Iva/AAI-8937-2020; Gasparetto, Marco/AFP-5871-2022; Hussey, Seamus/F-8810-2015; Mata Lopez, Laura/GSI-6751-2022; Romano, Claudio/L-1950-2016; Henderson, Paul/AAK-7867-2021; Hussey, Séamus/F-8810-2015; 康, 奔/JMQ-0812-2023; Rolandsdotter, Helena/J-5146-2019; Serban, Elena Daniela/V-4326-2017; Alvisi, Patrizia/HLH-2943-2023 15924580900; 56230811200; 37861301600; 57203231245; 23989876100; 7801459343; 6504767930; 56016081800; 57193544549; 22950164000; 33067537800; 55923616600; 7003266892; 55207312500; 57217485861; 8964706700; 57190369663; 6603884926; 26030040500; 57191440581; 37761333200; 57194823199; 58831924600; 36942786700; 16550408000; 7102531252; 9269163300; 25636843300; 58830568300; 57200084640; 36141572100; 58832387800; 7006209608; 56262375400; 57223946362; 9333600100; 6701638121; 56024272300 dror.shouval@gmail.com; JOURNAL OF CROHNS & COLITIS J CROHNS COLITIS 1873-9946 1876-4479 17 12 SCIE GASTROENTEROLOGY & HEPATOLOGY 2023 8.3 9.4 0.77 2025-06-25 5 3 Children; inflammatory bowel disease; proctitis; ulcerative colitis EVIDENCE-BASED CONSENSUS; DISEASE EXTENSION; COLITIS; MANAGEMENT; MESALAMINE; PREDICTORS; DIAGNOSIS; EFFICACY; THERAPY; SAFETY Children; inflammatory bowel disease; proctitis; ulcerative colitis Adolescent; Biological Products; Child; Colitis, Ulcerative; Humans; Inflammatory Bowel Diseases; Pediatric ulcerative colitis; Proctitis; Retrospective Studies; aminosalicylic acid; C reactive protein; mesalazine; steroid; biological product; abdominal pain; adolescent; Article; child; colectomy; diarrhea; disease activity; disease exacerbation; erythrocyte sedimentation rate; female; follow up; hematochezia; human; inflammation; inflammatory bowel disease; male; multicenter study; multivariate analysis; pediatrics; proctitis; proportional hazards model; retrospective study; special situation for pharmacovigilance; ulcerative colitis; case report; clinical trial; complication; inflammatory bowel disease; proctitis; ulcerative colitis English 2023 2023-12-30 10.1093/ecco-jcc/jjad111 바로가기 바로가기 바로가기 바로가기
Meeting Abstract Combination treatment of biologics with immunomodulator increases risk of infection after discharge in biologics naive steroid refractory acute severe ulcerative colitis patient: A KASID multicenter study Kim, S. J.; Lee, J.; Kim, D. H.; Park, S. H.; Kim, E. S.; Kim, K. O.; Lee, Y. J.; Song, E. M.; Kim, D. S. Chosun Univ, Internal Med, Coll Med, Gwangju, South Korea; Chonnam Natl Univ Hosp, Internal Med, Gwangju, South Korea; Sch Med, Internal Med, Gwangju, South Korea; Asan Med Ctr, Internal Med, Seoul, South Korea; Kyungpook Natl Univ, Internal Med, Sch Med, Daegu, South Korea; Yeungnam Univ Hosp, Internal Med, Deagu, South Korea; Keimyung Univ Hosp, Internal Med, Daegu, South Korea; Ewha Womans Univ, Internal Med, Seoul Hosp, Seoul, South Korea; Konyang Univ Hosp, Internal Med, Daejeon, South Korea Park, Sun-Young/B-4634-2014; Lee, Yoojin/AAB-9799-2022 JOURNAL OF CROHNS & COLITIS J CROHNS COLITIS 1873-9946 1876-4479 17 SCIE GASTROENTEROLOGY & HEPATOLOGY 2023 8.3 9.4 0 English 2023 2023-02 바로가기 바로가기
Meeting Abstract Comparison of Adverse Events of SARS-CoV-2 Vaccination between Patients with Inflammatory Bowel Disease and Healthcare Workers Kim, J.; Sagong, M.; Lee, Y. J.; Kim, K. O.; Kim, E. S.; Kim, E. Y.; Jang, B. I. Keimyung Univ, Sch Med, Dept Internal Med, Daegu, South Korea; Yeungnam Univ, Coll Med, Dept Internal Med, Daegu, South Korea; Kyungpook Natl Univ, Sch Med, Dept Internal Med, Daegu, South Korea; Daegu Catholic Univ, Sch Med, Dept Internal Med, Daegu, South Korea Lee, Yoojin/AAB-9799-2022 JOURNAL OF CROHNS & COLITIS J CROHNS COLITIS 1873-9946 1876-4479 17 SCIE GASTROENTEROLOGY & HEPATOLOGY 2023 8.3 9.4 0 English 2023 2023-02 바로가기 바로가기
Article CycloZ Improves Hyperglycemia and Lipid Metabolism by Modulating Lysine Acetylation in KK-Ay Mice Background: CycloZ, a combination of cyclo-His-Pro and zinc, has anti-diabetic activity. However, its exact mode of action remains to be elucidated. Methods: KK-Ay mice, a type 2 diabetes mellitus (T2DM) model, were administered CycloZ either as a preventive intervention, or as a therapy. Glycemic control was evaluated using the oral glucose tolerance test (OGTT), and glycosylated hemoglobin (HbA1c) levels. Liver and visceral adipose tissues (VATs) were used for histological evaluation, gene expression analysis, and protein expression analysis. Results: CycloZ administration improved glycemic control in KK-Ay mice in both prophylactic and therapeutic studies. Lysine acetylation of peroxisome proliferator-activated receptor gamma coactivator 1-alpha, liver kinase B1, and nuclear factor-kappa B p65 was decreased in the liver and VATs in CycloZ-treated mice. In addition, CycloZ treatment improved mitochondrial function, lipid oxidation, and inflammation in the liver and VATs of mice. CycloZ treatment also increased the level of beta-nicotinamide adenine dinucleotide (NAD(+)), which affected the activity of deacetylases, such as sirtuin 1 (Sirt1). Conclusion: Our findings suggest that the beneficial effects of CycloZ on diabetes and obesity occur through increased NAD(+) synthesis, which modulates Sirt1 deacetylase activity in the liver and VATs. Given that the mode of action of an NAD(+) booster or Sirt1 deacetylase activator is different from that of traditional T2DM drugs, CycloZ would be considered a novel therapeutic option for the treatment of T2DM. Jeon, Jongsu; Lee, Dohyun; Kim, Bobae; Park, Bo-Yoon; Oh, Chang Joo; Kim, Min-Ji; Jeon, Jae-Han; Lee, In-Kyu; Park, Onyu; Baek, Seoyeong; Lim, Chae Won; Ryu, Dongryeol; Fang, Sungsoon; Auwerx, Johan; Kim, Kyong-Tai; Jung, Hoe-Yune NovMetaPharma Co Ltd, R&D Ctr, Seoul, South Korea; Pohang Univ Sci & Technol POSTECH, Dept Life Sci, 77 Cheongam Ro, Pohang 37673, South Korea; Kyungpook Natl Univ, Res Inst Aging & Metab, Daegu, South Korea; Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Dept Internal Med, Daegu, South Korea; Handong Global Univ, Sch Life Sci, Pohang, South Korea; Daegu Catholic Univ, Grad Sch, Dept Med, Gyongsan, South Korea; Sungkyunkwan Univ, Dept Mol Cell Biol, Sch Med, Suwon, South Korea; Sungkyunkwan Univ, Biomed Inst Convergence SKKU BICS, Suwon, South Korea; Yonsei Univ, Grad Sch Med Sci, Coll Med, Brain Korea Project 21, Seoul, South Korea; Yonsei Univ, Gangnam Severance Hosp, Severance Biomed Sci Inst, Coll Med, Seoul, South Korea; Swiss Fed Inst Technol Lausanne, Inst Bioengn, Lab Integrat Syst Physiol, CH-1015 Lausanne, Switzerland; Pohang Univ Sci & Technol POSTECH, Sch Interdisciplinary Biosci & Bioengn, 77 Cheongam Ro, Pohang 37673, South Korea FANG, Sungsoon/HKW-2279-2023; Ryu, Dongryeol/AAQ-3642-2020; Kim, Min-Ji/Z-5205-2019; Park, Mi-Kyung/J-9643-2017; Auwerx, Johan/ABE-9307-2021; Lee, In-Kyu/AAR-6374-2021 42461616500; 57200249792; 55569996000; 57191358558; 14049080600; 57206189095; 36910340400; 36071537600; 58528716400; 58529395100; 58158274300; 57201809600; 7402422501; 57414044100; 7409315595; 35484149000 admin.auwerx@epfl.ch;ktk@postech.ac.kr;elijah98@postech.ac.kr; DIABETES & METABOLISM JOURNAL DIABETES METAB J 2233-6079 2233-6087 47 5 SCIE ENDOCRINOLOGY & METABOLISM 2023 6.8 9.4 0.53 2025-06-25 3 4 Acetylation; Diabetes mellitus; type 2; NAD; Obesity NF-KAPPA-B; HIS-PRO; ADIPOSE-TISSUE; ZINC; SIRT1; CYCLO(HIS-PRO); MITOCHONDRIAL; ENZYMES; EXPRESSION; MOLECULES Acetylation; Diabetes mellitus, type 2; NAD; Obesity antidiabetic agent; cycloz; hemoglobin A1c; immunoglobulin enhancer binding protein; lysine; nicotinamide adenine dinucleotide; peroxisome proliferator activated receptor gamma coactivator 1alpha; protein kinase LKB1; sirtuin 1; unclassified drug; animal experiment; animal model; animal tissue; Article; controlled study; enzyme activity; gene expression profiling; glycemic control; hepatitis; histopathology; hyperglycemia; intra-abdominal fat; KK-Ay mouse; lipid metabolism; lipid oxidation; liver tissue; male; mitochondrion; mouse; non insulin dependent diabetes mellitus; nonhuman; obesity; oral glucose tolerance test; protein acetylation; protein expression English 2023 2023-09 10.4093/dmj.2022.0244 바로가기 바로가기 바로가기 바로가기
Meeting Abstract Development of an artificial intelligence model using stool pictures for predicting endoscopic mucosal inflammation in patients with Ulcerative Colitis; KASID multicenter study Kim, E. S.; Lee, J. W.; Kim, S. K.; Lee, H. S.; Lee, Y. J.; Kim, J.; Kim, K. O.; Jang, B. I.; Kim, E. Y.; Jung, Y. J.; Park, S. K.; Park, D. I.; Woo, D.; Jeong, S. Kyungpook Natl Univ Hosp, Internal Med, Daegu, South Korea; Keimyung Univ, Internal Med, Sch Med, Daegu, South Korea; Yeungnam Univ, Internal Med, Coll Med, Daegu, South Korea; Daegu Catholic Univ, Internal Med, Sch Med, Daegu, South Korea; Sungkyunkwan Univ, Internal Med, Sch Med, Kangbuk Samsung Hosp, Seoul, South Korea; Kyungpook Natl Univ Hosp, Med Informat, Res Ctr Artificial Intelligence Med, Daegu, South Korea Lee, Yoojin/AAB-9799-2022 JOURNAL OF CROHNS & COLITIS J CROHNS COLITIS 1873-9946 1876-4479 17 SCIE GASTROENTEROLOGY & HEPATOLOGY 2023 8.3 9.4 0 English 2023 2023-02 바로가기 바로가기
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WoS Web of Science. Clarivate Analytics에서 제공하는 학술 데이터베이스입니다. 해당 논문이 WoS에 수록되어 있는지 여부를 표시합니다 (○: 수록됨).
SCOPUS Elsevier에서 제공하는 세계 최대 규모의 초록 및 인용 데이터베이스입니다. 해당 논문이 SCOPUS에 수록되어 있는지 여부를 표시합니다 (○: 수록됨).
Document Type 문헌의 유형을 나타냅니다. Article(원저), Review(리뷰), Proceeding Paper(학회논문), Editorial Material(편집자료), Letter(레터) 등으로 분류됩니다.
Title 논문의 제목입니다.
Abstract 논문의 초록(요약)입니다. 연구의 목적, 방법, 결과, 결론을 간략히 요약한 내용입니다.
Authors 논문의 저자 목록입니다. 공동 저자가 여러 명인 경우 세미콜론(;)으로 구분됩니다.
Affiliation 저자들의 소속 기관 정보입니다. 대학, 연구소, 기업 등 저자가 소속된 기관명이 표시됩니다.
ResearcherID (WoS) Web of Science의 고유 연구자 식별번호입니다. 동명이인을 구분하고 연구자의 업적을 정확하게 추적할 수 있습니다.
AuthorsID (SCOPUS) SCOPUS의 고유 저자 식별번호입니다. 연구자의 모든 출판물을 추적하고 관리하는 데 사용됩니다.
Journal 논문이 게재된 학술지의 정식 명칭입니다.
JCR Abbreviation Journal Citation Reports에서 사용하는 저널의 공식 약어입니다. 저널을 간략하게 표기할 때 사용됩니다.
ISSN International Standard Serial Number. 국제표준연속간행물번호로, 인쇄본 저널에 부여되는 고유 식별번호입니다.
eISSN Electronic ISSN. 전자 버전 저널에 부여되는 고유 식별번호입니다.
Volume 저널의 권(Volume) 번호입니다. 보통 연도별로 하나의 권이 부여됩니다.
Issue 저널의 호(Issue) 번호입니다. 한 권 내에서 여러 호로 나누어 출판되는 경우가 많습니다.
WoS Edition Web of Science의 에디션입니다. SCIE(Science Citation Index Expanded), SSCI(Social Sciences Citation Index), AHCI(Arts & Humanities Citation Index) 등으로 구분됩니다.
WoS Category Web of Science의 주제 분류 카테고리입니다. 저널과 논문이 속한 학문 분야를 나타냅니다.
JCR Year 해당 저널의 JCR(Journal Citation Reports) 지표가 산출된 연도입니다.
IF (Impact Factor) 저널 영향력 지수. 최근 2년간 발표된 논문이 해당 연도에 평균적으로 인용된 횟수를 나타냅니다. 저널의 학술적 영향력을 나타내는 대표적인 지표입니다.
JCR (%) 해당 카테고리에서 저널이 위치하는 상위 백분율입니다. 값이 낮을수록 우수한 저널임을 의미합니다 (예: 5%는 상위 5%를 의미).
FWCI Field-Weighted Citation Impact. 분야별 가중 인용 영향력 지수입니다. 논문이 받은 인용을 동일 분야, 동일 연도, 동일 문헌 유형의 평균과 비교한 값입니다. 1.0이 평균이며, 1.0보다 높으면 평균 이상의 인용을 받았음을 의미합니다.
FWCI UpdateDate FWCI 값이 마지막으로 업데이트된 날짜입니다. FWCI는 인용이 누적됨에 따라 주기적으로 업데이트됩니다.
WOS Citation Web of Science에서 집계된 해당 논문의 총 인용 횟수입니다.
SCOPUS Citation SCOPUS에서 집계된 해당 논문의 총 인용 횟수입니다.
Keywords (WoS) 저자가 논문에서 직접 지정한 키워드입니다. Web of Science에 등록된 저자 키워드 목록입니다.
KeywordsPlus (WoS) Web of Science에서 자동으로 추출한 추가 키워드입니다. 논문의 참고문헌 제목에서 자주 등장하는 단어들로 생성됩니다.
Keywords (SCOPUS) 저자가 논문에서 직접 지정한 키워드입니다. SCOPUS에 등록된 저자 키워드 목록입니다.
KeywordsPlus (SCOPUS) SCOPUS에서 자동으로 추출하거나 추가한 색인 키워드입니다.
Language 논문이 작성된 언어입니다. 대부분 English이며, 그 외 다양한 언어로 작성된 논문이 포함될 수 있습니다.
Publication Year 논문이 출판된 연도입니다.
Publication Date 논문의 정확한 출판 날짜입니다 (년-월-일 형식).
DOI Digital Object Identifier. 디지털 객체 식별자로, 논문을 고유하게 식별하는 영구적인 식별번호입니다. 이를 통해 논문의 온라인 위치를 찾을 수 있습니다.