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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | Meeting Abstract | TREATMENT ADHERENCE AS A COST DETERMINANT IN PATIENTS WITH TUBERCULOSIS | Kwon, S. H.; Nam, J. H.; Min, S.; Kim, H. L.; Kwon, J. W. | Sungkyunkwan Univ, Suwon, South Korea; Sahmyook Univ, Seoul, South Korea; Kyungpook Natl Univ, Daegu, South Korea | VALUE IN HEALTH | VALUE HEALTH | 1098-3015 | 1524-4733 | 25 | 1 | SSCI;SCIE | ECONOMICS;HEALTH CARE SCIENCES & SERVICES;HEALTH POLICY & SERVICES | 2022 | 4.5 | 8.6 | 0 | English | 2022 | 2022-01 | 바로가기 | 바로가기 | ||||||||||||||||
| ○ | ○ | Article | Ultrasonically dispersed multi-composite strategy of NiCo2S4/Halloysite nanotubes/carbon: An efficient solid-state hybrid supercapacitor and hydrogen evolution reaction material | Herein, we have developed a novel hybrid material based on NiCo2S4 (NCS), halloysite nanotubes (HNTs), and carbon as promising electrodes for supercapacitors (SCs). Firstly, mesoporous NCS nanoflakes were prepared by co-precipitation method followed by physically mixing with HNTs and carbon, and screen printed on nickel foam. After ultrasonication, a uniform distribution of the Carbon/HNTs complex was observed, which was confirmed by surface morphological analysis. When used as electrode material, the NCS/HNTs/C hybrid displayed a maximum specific capacity of 544 mAh g(-1) at a scan rate of 5 mV s(-1). Later, a solid-state hybrid SCs was fabricated using activated carbon (AC) as the negative and NCS/HNTs/C as the positive electrode (NCS/HNTs/C//AC). The device delivers a high energy density of 42.66 Wh kg(-1) at a power density of 8.36 kW kg(-1). In addition, the device demonstrates long-term cycling stability. Furthermore, the optimized NCS, NCS/HNTs, and NCS/HNTs/C nanocomposites also presented superior hydrogen evolution reaction (HER) performance of 201, 169, and 116 mV in the acidic bath at a current density of 10 mA cm(-2), respectively. Thus, the synthesis of NCS/HNTs/C nanocomposite as positive electrodes for hybrid SCs opens new opportunities for the development of next-generation high energy density SCs. | Shinde, Surendra K.; Dubal, Deepak P.; Yadav, Hemraj M.; Jagadale, Ajay D.; Maile, Nagesh; Karade, Swapnil S.; Lee, Dae-Sung; Kim, Dae-Young | Dongguk Univ Seoul, Dept Biol & Environm Sci, 32 Dongguk Ro, Goyang Si 10326, Gyeonggi Do, South Korea; Queensland Univ Technol QUT, Sch Chem Phys & Mech Engn, 2 George St, Brisbane, Qld 4001, Australia; Shivaji Univ Kolhapur, Sch Nanosci & Biotechnol, Kolhapur 416004, Maharashtra, India; SASTRA Deemed Univ, Ctr Energy Storage & Convers, Sch Elect & Elect Engn, Thanjavur 613401, Tamil Nadu, India; Kyungpook Natl Univ, Dept Environm Engn, 80 Daehak Ro, Daegu 41566, South Korea; Univ Southern Denmark, Dept Green Technol, Campusvej 55, DK-5230 Odense M, Denmark | Dubal, Deepak/I-1927-2015; Shinde, Santaji/LFT-9528-2024; Karade, Swapnil/AAA-8285-2020; Maile, Nagesh/AFD-8838-2022; Jagadale, Ajay/O-5253-2014 | 55776351000; 26645834500; 55389590800; 44161199800; 57193804539; 56674484600; 55568524907; 55742887400 | sbpkim@dongguk.edu; | CERAMICS INTERNATIONAL | CERAM INT | 0272-8842 | 1873-3956 | 48 | 17 | SCIE | MATERIALS SCIENCE, CERAMICS | 2022 | 5.2 | 8.6 | 0.55 | 2025-06-25 | 6 | 7 | NiCo2S4; Ultrasonication treatment; Halloysites; Carbon; Hybrid supercapacitors; Solid-state hybrid device; Hydrogen evolution reaction | NI FOAM; ELECTROCHEMICAL PERFORMANCE; ELECTRODE MATERIAL; CHEMICAL-SYNTHESIS; HIGHLY EFFICIENT; FACILE SYNTHESIS; ENERGY-STORAGE; THIN-FILMS; NICO2S4; NANOFLAKES | Carbon; Halloysites; Hybrid supercapacitors; Hydrogen evolution reaction; NiCo<sub>2</sub>S<sub>4</sub>; Solid-state hybrid device; Ultrasonication treatment | Activated carbon; Electrodes; Foams; Hybrid materials; Hydrogen; Kaolinite; Nanocomposites; Nanotubes; Precipitation (chemical); Supercapacitor; Halloysite; Halloysite nanotubes; Higher energy density; Hybrid devices; Hybrid supercapacitors; Hydrogen evolution reactions; Positive electrodes; Solid-state hybrid device; Ultra-sonication; Ultrasonication treatment; Nickel compounds | English | 2022 | 2022-09-01 | 10.1016/j.ceramint.2022.05.156 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Association of Serum Glucose, Serotonin, Aspartate Aminotransferase, and Calcium Levels with Meat Quality and Palatability Characteristics of Broiler Pectoralis Major Muscle | Simple Summary Apoptosis is a critical and highly regulated cell death process after exsanguination, and serum apoptosis-related molecules are associated with apoptotic and glycolytic potentials. Thus, the activities and production of these molecules can directly and/or indirectly affect variation in meat quality characteristics. However, there is limited information on the complex relationship between serum biochemical molecules and their effects on the meat and eating quality of broilers. In the present study, the activity of aspartate aminotransferase, a cytoprotective molecule, was found to be associated with other apoptosis-related molecules, including blood glucose, serotonin, and calcium levels, at exsanguination. All serum parameters analyzed in this study were related to the early postmortem and ultimate muscle pH. Thus, apoptosis-related molecules may affect the glycolytic rate in broiler pectoralis major muscle. Additionally, our results demonstrated that the variations in the sensory quality traits of broilers could be somewhat explained by the levels of apoptosis-related serum parameters at exsanguination. This study investigated the correlations between apoptosis-related blood biochemical parameters measured at exsanguination and the meat and sensory quality characteristics of broiler pectoralis major muscle. The concentration of serotonin showed a positive correlation with aspartate aminotransferase (AST) activity (p < 0.001) and a negative correlation with calcium content (p < 0.01). All serum parameters showed relationships with muscle pH at 15 min and/or 24 h postmortem (p < 0.05). Serum AST activity, which had a negative correlation with calcium content (p < 0.01), was positively related with muscle pH and negatively correlated with Warner-Bratzler shear force values (WBS, p < 0.05). Principal component analysis results revealed the associations between AST activity and meat quality traits, including pH(24h), lightness, and WBS. Furthermore, cooked breast with higher AST activity and lower calcium level tended to exhibit higher scores of tenderness and overall acceptability than that with lower AST activity and higher calcium level (p < 0.05). | Lee, Boin; Choi, Young Min | Kyungpook Natl Univ, Dept Anim Sci & Biotechnol, Sangju Si 37224, South Korea | Choi, Young/J-6027-2014 | 57218130871; 57226673843 | ananassab@knu.ac.kr;ymchoi1@knu.ac.kr; | ANIMALS | ANIMALS-BASEL | 2076-2615 | 12 | 12 | SCIE | AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES | 2022 | 3 | 8.7 | 0.61 | 2025-06-25 | 4 | 3 | serum biochemical parameters; apoptosis; meat quality; palatability; chicken breast | STRESS; SLAUGHTER; TRANSPORT; TRAITS; PALE; SOFT | apoptosis; chicken breast; meat quality; palatability; serum biochemical parameters | aspartate aminotransferase; calcium; cytoprotective agent; glucose; glycolytic enzyme; serotonin; apoptosis; Article; aspartate aminotransferase blood level; autopsy; broiler; canned food; chicken; clinical article; colorimetry; controlled study; enzyme activity; enzyme linked immunosorbent assay; exsanguination; food quality; glucose blood level; human; human tissue; male; meat; nonhuman; pectoralis major muscle; pH; principal component analysis; quality control; regulated cell death; sensory evaluation; urea nitrogen blood level; whole body scintiscanning | English | 2022 | 2022-06 | 10.3390/ani12121567 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Associations of Apoptotic and Anti-Apoptotic Factors with Beef Quality, Histochemical Characteristics, and Palatability of Hanwoo Longissimus thoracis Muscle | Simple Summary After the onset of muscle fiber apoptosis, various apoptotic and anti-apoptotic factors are selectively increased. These factors are directly and/or indirectly associated with tenderness variation. Given the complex relationship between the apoptosis-related molecules, more data are needed on how the expression patterns of these factors during the post-mortem period affects the palatability of beef. In this study, the combination of expression levels of apoptotic (cytochrome c and caspases) and anti-apoptotic (small heat shock proteins) factors at 45 min post-mortem tended to be associated with Warner-Bratzler shear force and tenderness attributes in Hanwoo steers with a lower degree of marbling. Meanwhile, levels of these molecules at 24 h had a somewhat limited effect on the beef tenderness. Clearly, these results demonstrate that expression levels of apoptotic and anti-apoptotic molecules at the early post-mortem period may be a relevant indicator to explain the tenderness variation in Hanwoo. This study compared the meat quality, histochemical traits, palatability, and expression levels of apoptotic (cytochrome c and caspases) and anti-apoptotic (small heat shock proteins) factors at 45 min and 24 h post-mortem of Hanwoo Longissimus thoracis muscles in groups categorized by Warner-Bratzler shear force (WBS) values to investigate the association between beef tenderness variation and apoptosis-related molecules. There were no differences in marbling scores, meat quality traits, or histochemical characteristics among the WBS groups (p > 0.05) indicating no significant effect on the tenderness variation in the current study. On the other hand, the low group exhibited higher levels of apoptotic and anti-apoptotic factors (except for alpha beta-crystallin) at 45 min post-mortem compared to the high WBS group, resulting in higher scores of tenderness attributes (p 0.05) except for heat shock protein 27 (p < 0.05). | Lee, Boin; Kim, Jae-Yeong; Choi, Young-Min | Kyungpook Natl Univ, Dept Anim Sci & Biotechnol, Sangju Si 37224, South Korea | Choi, Young/J-6027-2014 | 57218130871; 57226306561; 57226673843 | ananassab@knu.ac.kr;rlarbgml0603@naver.com;ymchoi1@knu.ac.kr; | ANIMALS | ANIMALS-BASEL | 2076-2615 | 12 | 4 | SCIE | AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES | 2022 | 3 | 8.7 | 1.83 | 2025-06-25 | 9 | 10 | tenderness; apoptosis; caspases; cytochrome c; small heat shock proteins; Hanwoo | HEAT-SHOCK PROTEINS; SENSORY QUALITY; MEAT TENDERNESS; POSTMORTEM; CASPASE-3; ACTIVATION; IDENTIFICATION; PROTEOLYSIS; SYSTEMS | Apoptosis; Caspases; Cytochrome c; Hanwoo; Small heat shock proteins; Tenderness | beta crystallin; caspase; caspase 3; caspase 7; caspase 9; cytochrome c; glyceraldehyde 3 phosphate dehydrogenase; heat shock protein 27; muscle protein; small heat shock protein; animal experiment; animal tissue; apoptosis; Article; cattle breed; hanwoo; histochemistry; longissimus thoracis; nonhuman; palatability; polyacrylamide gel electrophoresis; protein expression; real time polymerase chain reaction; RNA isolation; sensory analysis; shear flow; solvent extraction; Soxhlet extraction; Warner-Bratzler shear force; Western blotting | English | 2022 | 2022-02 | 10.3390/ani12040467 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Characterization of Virulence Factors in Enterotoxin-Producing Staphylococcus aureus from Bulk Tank Milk | Simple Summary Staphylococcus aureus, apathogen that causes bovine mastitis, produces various virulence factors, and human consumption of milk contaminated with the S. aureus enterotoxin may pose a public health risk. This study analyzed the genetic characteristics of bovine-mastitis-related virulence factors to evaluate the potential pathogenesis of S. aureus isolated from bulk tank milk. The results show that S. aureus isolated from bulk tank milk, not from mastitis, had a high prevalence of virulence factors and that the high presence of enterotoxins may be due to poor hygiene. Therefore, developing a strong monitoring and sanitation program for dairy factories is important to ensure hygienic milk production. Staphylococcus aureus, a persistent mastitis-causing pathogen, produces various virulence factors, including enterotoxins. This study analyzed the genetic characteristics of bovine-mastitis-related virulence factors to evaluate the potential pathogenesis of S. aureus isolated from bulk tank milk. Among 93 S. aureus isolates from 396 dairy farms operated by 3 dairy companies in Korea, 40 (43.0%) isolates carried one or more enterotoxin genes. Moreover, S. aureus carrying enterotoxin genes showed a higher prevalence in all virulence genes tested in this study except for pvl and lukM, which were not detected in any isolate, than in the isolates without enterotoxin genes. In particular, the prevalence of six genes (hla, hlb, lukED, fnbA, clfA, and clfB) was significantly higher in S. aureus carrying the enterotoxin genes than in the isolates without the enterotoxin genes (p < 0.05). The most common multilocus sequence type of enterotoxin-producing isolates was ST188, and all isolates of ST188 harbored the see gene. S. aureus isolated from bulk tank milk, not from mastitis, had a high prevalence of virulence factors, posing a public health threat. Moreover, a high presence of enterotoxins in bulk tank milk is probably because of poor hygiene; therefore, it is important to develop strong monitoring and sanitation programs for dairy factories. | Jung, Hye-Ri; Lee, Young Ju | Kyungpook Natl Univ, Coll Vet Med, Daegu 41566, South Korea; Kyungpook Natl Univ, Zoonoses Res Inst, Daegu 41566, South Korea | 57265190900; 56612134500 | heelee1214@naver.com;youngju@knu.ac.kr; | ANIMALS | ANIMALS-BASEL | 2076-2615 | 12 | 3 | SCIE | AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES | 2022 | 3 | 8.7 | 1.42 | 2025-06-25 | 6 | 7 | bulk tank milk; enterotoxin; virulence factor; bovine mastitis | PANTON-VALENTINE LEUKOCIDIN; BOVINE MASTITIS; RAW-MILK; GENES; INFECTIONS; PREVALENCE; RESISTANCE; ADHESION | Bovine mastitis; Bulk tank milk; Enterotoxin; Virulence factor | enterotoxin; gentamicin; leukocidin; trimethoprim; virulence factor; antibiotic sensitivity; Article; bacterial gene; bacterial virulence; genetic trait; hygiene; milk production; molecular phylogeny; molecular typing; multilocus sequence typing; pathogenesis; pathogenicity island; polymerase chain reaction; public health service; quorum sensing; septic shock; Staphylococcus aureus; toxic shock syndrome | English | 2022 | 2022-02 | 10.3390/ani12030301 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Contribution of tetrodotoxin-resistant persistent Na⁺ currents to the excitability of C-type dural afferent neurons in rats | Background Growing evidence supports the important role of persistent sodium currents (I-NaP) in the neuronal excitability of various central neurons. However, the role of tetrodotoxin-resistant (TTX-R) Na+ channel-mediated I-NaP in the neuronal excitability of nociceptive neurons remains poorly understood. Methods We investigated the functional role of TTX-R I-NaP in the excitability of C-type nociceptive dural afferent neurons, which was identified using a fluorescent dye, 1,1 '-dioctadecyl-3,3,3 ',3 '-tetramethylindocarbocyanine perchloride (DiI), and a whole-cell patch-clamp technique. Results TTX-R I-NaP were found in most DiI-positive neurons, but their density was proportional to neuronal size. Although the voltage dependence of TTX-R Na+ channels did not differ among DiI-positive neurons, the extent of the onset of slow inactivation, recovery from inactivation, and use-dependent inhibition of these channels was highly correlated with neuronal size and, to a great extent, the density of TTX-R I-NaP. In the presence of TTX, treatment with a specific I-NaP inhibitor, riluzole, substantially decreased the number of action potentials generated by depolarizing current injection, suggesting that TTX-R I-NaP are related to the excitability of dural afferent neurons. In animals treated chronically with inflammatory mediators, the density of TTX-R I-NaP was significantly increased, and it was difficult to inactivate TTX-R Na+ channels. Conclusions TTX-R I-NaP apparently contributes to the differential properties of TTX-R Na+ channels and neuronal excitability. Consequently, the selective modulation of TTX-R I-NaP could be, at least in part, a new approach for the treatment of migraine headaches. | Nakamura, Michiko; Jang, Il-Sung | Kyungpook Natl Univ, Sch Dent, Dept Pharmacol, Daegu 700412, South Korea; Kyungpook Natl Univ, Brain Sci & Engn Inst, Daegu 700412, South Korea | 36051866000; 7102177910 | jis7619@knu.ac.kr; | JOURNAL OF HEADACHE AND PAIN | J HEADACHE PAIN | 1129-2369 | 1129-2377 | 23 | 1 | SCIE | CLINICAL NEUROLOGY;NEUROSCIENCES | 2022 | 7.4 | 8.7 | 0.96 | 2025-06-25 | 7 | 7 | Migraine; Dural afferent neurons; TTX-R Na+ channels; Persistent Na+ currents; Patch-clamp | MEDIATOR-INDUCED SENSITIZATION; DISTINCT FIRING PROPERTIES; SODIUM CURRENT; SLOW INACTIVATION; DEPENDENT INHIBITION; BURST GENERATION; RAMP CURRENTS; V NEURONS; CHANNELS; NA(V)1.8 | Dural afferent neurons; Migraine; Patch-clamp; Persistent Na<sup>+</sup> currents; TTX-R Na<sup>+</sup> channels | Animals; Membrane Potentials; Neurons, Afferent; Rats; Rats, Sprague-Dawley; Sodium; Sodium Channels; Tetrodotoxin; 1,1' dioctadecyl 3,3,3',3' tetramethylindocarbocyanine; 4 (n ethyl n phenylamino) 1,2 dimethyl 6 (methylamino)pyrimidinium chloride; a 803467; bradykinin; calcitonin gene related peptide; collagenase; dizocilpine; doxycycline; ethylene vinyl acetate copolymer; fibroblast growth factor 2; histamine; ketamine; naproxen; penicillin G; prostaglandin E2; riluzole; sodium channel Nav1.8; tetrodotoxin; trypsin; unclassified drug; vanilloid receptor 1; xylazine; sodium; sodium channel; tetrodotoxin; action potential; adult; animal cell; animal experiment; animal model; Article; cell size; controlled study; correlation analysis; craniotomy; depolarization; electric potential; female; fluorescence microscopy; male; membrane current; migraine; nerve cell; nerve cell excitability; nociception; nonhuman; rat; sensory nerve cell; sodium current; whole cell patch clamp; animal; membrane potential; metabolism; physiology; Sprague Dawley rat | English | 2022 | 2022-12 | 10.1186/s10194-022-01443-7 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Differential Expression of MSTN Isoforms in Muscle between Broiler and Layer Chickens | Simple Summary Increasing the growth rate of food animals with a concomitant increase in muscle yield is a primary goal of animal producers. The identification of factors and a better understanding of the underlying mechanisms of these factors for muscle growth are important if we are to improve meat production in domestic animals. In avian species, MSTN-A has anti-myogenic activities and MSTN-B functions as a pro-myogenic factor. In this study, the expression of the Mstn isoforms and the sizes of myofibers and muscle bundles during development were analyzed in broiler and layer chicken breeds. Although the expression levels of total Mstn were not different between the two breeds, the ratios of Mstn-B to -A were significantly higher in the broilers with muscle hypertrophy and hyperplasia compared to the layers. Further characterizations of the muscle in broilers revealed a greater bundle area but similar fiber number per bundle compared to the layers. Our study demonstrated higher ratios of Mstn-B/-A expression in the muscles of broiler chickens, suggesting that the development of strategies to enhance the expression of Mstn-B will lead to increased muscle growth and poultry production. Myostatin (Mstn)-A, the main isoform among Mstn splicing variants, functions as a negative regulator, whereas Mstn-B functions as a positive regulator in muscle development. Because broiler chickens are a fast-growing breed raised for meat production and layer chickens are a slow-growing breed raised for egg production, differences in the expression of Mstn isoforms between the two distinct breeds were analyzed in this study. There was no difference in the expression levels of total Mstn (Mstn-A and -B forms) during embryonic development and at D33 between the two breeds. Interestingly, the ratios of Mstn-B to -A were significantly higher in the broiler compared to the layer at most ages. In pectoralis major muscle (PM) tissue, the cross-sectional area (CSA) of muscle fiber was significantly greater in the broiler. The broiler also showed greater bundle CSA and a similar fiber number per bundle compared to the layer at D5 and D33. These data suggest that the greater bundle CSA with myofiber hypertrophy in the broilers is associated with greater muscle growth. The relationship between the expression of Mstn isoforms and growth rate can be used as a potential genetic marker for the selection of higher muscle growth in chickens. | Kim, Dong-Hwan; Choi, Young Min; Lee, Joonbum; Shin, Sangsu; Kim, Sanggu; Suh, Yeunsu; Lee, Kichoon | Ohio State Univ, Dept Anim Sci, Columbus, OH 43210 USA; Kyungpook Natl Univ, Dept Anim Sci, Sangju 37224, South Korea; Ohio State Univ, Interdisciplinary PhD Program Nutr, Columbus, OH 43210 USA; Kyungpook Natl Univ, Dept Anim Biotechnol, Sangju 37224, South Korea; Ohio State Univ, Dept Vet Biosci, Coll Vet Med, Columbus, OH 43210 USA | Choi, Young/J-6027-2014; Lee, Suhyun/HOF-4644-2023; Kim, Donghwan/LRC-0840-2024; Lee, Kichoon/G-2234-2012; Kim, Sanggu/KPA-0398-2024 | 56621562800; 57226673843; 57194466213; 55490360000; 37021558600; 26430117900; 14123395400 | kim.4094@osu.edu;ymchoi1@knu.ac.kr;lee.3920@osu.edu;sss@knu.ac.kr;kim.6477@osu.edu;suh.83@osu.edu;lee.2626@osu.edu; | ANIMALS | ANIMALS-BASEL | 2076-2615 | 12 | 5 | SCIE | AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES | 2022 | 3 | 8.7 | 2.04 | 2025-06-25 | 12 | 10 | myostatin isoforms; chicken; broiler; layer; muscle hypertrophy | MYOSTATIN; GROWTH | Broiler; Chicken; Layer; Muscle hypertrophy; Myostatin isoforms | myostatin; myostatin A; myostatin B; RNA isoform; unclassified drug; animal cell; animal experiment; animal tissue; Article; broiler; chicken; controlled study; egg production; embryo; female; genetic variability; histology; male; mRNA expression level; muscle cell; nonhuman; pectoralis major muscle; protein expression; real time polymerase chain reaction | English | 2022 | 2022-03 | 10.3390/ani12050539 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Distribution of CRISPR in Escherichia coli Isolated from Bulk Tank Milk and Its Potential Relationship with Virulence | Simple Summary In the dairy farms of many different countries, E. coli is one of the most common causes of mastitis. It is defined as mammary pathogenic E. coli, and is known to cause opportunistic infections by possessing diverse virulence factors. Therefore, the purpose of this study was to investigate the virulence potential of E. coli isolates from bulk tank milk in Korea, and observe its association with clustered regularly interspaced short palindromic repeat (CRISPR) arrays. The results showed that out of 183 isolates, 164 (89.6%) possessed one or more of 18 virulence genes, and belonged to phylogenetic groups B1 (64.0%), A (20.1%), D (8.5%), and C (7.3%). CRISPR arrays of E. coli are classified as either CRISPR I-E (CRISPR 1 and 2) or CRISPR I-F (CRISPR 3 and 4). In this study, only CRISPR 1 (95.7%) and 2 (74.4%) were detected. Among the eight protospacers matching plasmids and phages, three were associated with gene regulation, and one was associated with virulence. Moreover, the different virulence genes showed significantly different patterns of CRISPR distribution and CRISPR sequence-types. This result implies that CRISPR loci may be associated with gene regulation and pathogenicity in E. coli, and that the CRISPR sequence-typing approach can help to clarify and trace virulence potential, even though the E. coli isolates were from normal bulk tank milk. Escherichia coli is one of the most common causes of mastitis on dairy farms around the world, but its clinical severity is determined by a combination of virulence factors. Recently, clustered regularly interspaced short palindromic repeat (CRISPR) arrays have been reported as a novel typing method because of their usefulness in discriminating pathogenic bacterial isolates. Therefore, this study aimed to investigate the virulence potential of E. coli isolated from bulk tank milk, not from mastitis, and to analyze its pathogenic characterization using the CRISPR typing method. In total, 164 (89.6%) out of 183 E. coli isolated from the bulk tank milk of 290 farms carried one or more of eighteen virulence genes. The most prevalent virulence gene was fimH (80.9%), followed by iss (38.3%), traT (26.8%), ompT (25.7%), afa/draBC (24.0%), and univcnf (21.9%). Moreover, the phylogenetic group with the highest prevalence was B1 (64.0%), followed by A (20.1%), D (8.5%), and C (7.3%) (p < 0.05). Among the four CRISPR loci, only two, CRISPR 1 and CRISPR 2, were found. Interestingly, the distribution of CRISPR 1 was significantly higher in groups A and B1 compared to that of CRISPR 2 (p < 0.05), but there were no significant differences in groups C and D. The prevalence of CRISPR 1 by virulence gene ranged from 91.8% to 100%, whereas that of CRISPR 2 ranged from 57.5% to 93.9%. The distribution of CRISPR 1 was significantly higher in fimH, ompT, afa/draBC, and univcnf genes than that of CRISPR 2 (p < 0.05). The most prevalent E. coli sequence types (EST) among 26 ESTs was EST 22 (45.1%), followed by EST 4 (23.2%), EST 16 (20.1%), EST 25 (19.5%), and EST 24 (18.3%). Interestingly, four genes, fimH, ompT, afa/draBC, and univcnf, had a significantly higher prevalence in both EST 4 and EST 22 (p < 0.05). Among the seven protospacers derived from CRISPR 1, protospacer 163 had the highest prevalence (20.4%), and it only existed in EST 4 and EST 22. This study suggests that the CRISPR sequence-typing approach can help to clarify and trace virulence potential, although the E. coli isolates were from normal bulk tank milk and not from mastitis. | Kang, Hyo-Jung; Lee, Young-Ju | Kyungpook Natl Univ, Coll Vet Med, Daegu 41566, South Korea; Kyungpook Natl Univ, Zoonoses Res Inst, Daegu 41566, South Korea | 57224364605; 56612134500 | sa01083@knu.ac.kr;youngju@knu.ac.kr; | ANIMALS | ANIMALS-BASEL | 2076-2615 | 12 | 4 | SCIE | AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES | 2022 | 3 | 8.7 | 0.61 | 2025-06-25 | 2 | 3 | E; coli; virulence gene; phylogeny; CRISPR array; bulk tank milk | BOVINE MASTITIS; ANTIMICROBIAL RESISTANCE; PHYLOGENETIC GROUP; CLINICAL MASTITIS; VI SECRETION; SEROTYPES; STRAINS; IMMUNITY; GENES; ASSOCIATION | Bulk tank milk; CRISPR array; E. coli; Phylogeny; Virulence gene | virulence factor; allele; antibiotic sensitivity; Article; bacterial gene; bacterial virulence; bacterium isolate; bacterium isolation; broth dilution; clustered regularly interspaced short palindromic repeat; controlled study; Escherichia coli; major clinical study; mastitis; microarray analysis; milk; nonhuman; phylogeny; plasmid; polymerase chain reaction; prevalence; randomized controlled trial; sequence analysis | English | 2022 | 2022-02 | 10.3390/ani12040503 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Frequency of an X-Linked Maternal Variant of the Bovine FOXP3 Gene Associated with Infertility in Different Cattle Breeds: A Pilot Study | Immune adaptation plays an essential role in determining pregnancy, which has been shown to be dependent on sufficient immunological tolerance mediated by FOXP3(+) regulatory T cells. Recently, an X-linked maternal single-nucleotide polymorphism (SNP), located 2175 base pairs upstream of the start codon in the bovine FOXP3 gene (NC_037357.1: g.87298881A>G, rs135720414), was identified in Japanese Black (JB: Bos taurus) cows in association with recurrent infertility. However, with the exception of JB cows, the frequency of this SNP has yet to be studied in other cow populations. In this study, we thus aimed to evaluate the frequency of this SNP in different cow breeds. Between 2018 and 2021, a total of 809 DNA samples were obtained from 581 JB, 73 Holstein Friesian (HF: B. taurus), 125 Korean Hanwoo (KH: B. taurus coreanae), and 30 Indonesian Madura (IM: a crossbreed between B. indicus and B. javanicus) cows, which were genotyped using a TaqMan probe-based real-time polymerase chain reaction assay designed in this study. The frequency of the G allele was found to be relatively high in local IM (0.700), moderate in dairy HF (0.466), and low in beef JB (0.250) and KH (0.112) cows, with differences in the frequencies between each group being shown to be statistically significant (p < 0.005) using Fisher's exact test. The results obtained in this study indicate that the G allele frequencies of the identified the SNP differ markedly in different breeds of taurine and indicine cattle. Given these findings, it would thus be important to evaluate the relationships between high frequencies of the G allele and infertility in different breeds. | Islam, Md Shafiqul; Takagi, Mitsuhiro; Lee, Keun-Woo; Chang, Hye-Sook; Okawa, Hiroaki; Yunus, Muchammad; Lestari, Tita Damayanti; Tacharina, Martia Rani; Pervin, Shahnaj; Rakib, Tofazzal Md; Yabuki, Akira; Yamato, Osamu | Kagoshima Univ, Joint Fac Vet Med, Lab Clin Pathol, 1-21-24 Korimoto, Kagoshima 8900065, Japan; Chattogram Vet & Anim Sci Univ, Fac Vet Med, Dept Pathol & Parasitol, Khulshi 4225, Chattogram, Bangladesh; Yamaguchi Univ, Joint Fac Vet Med, Lab Theriogenol, 1677-1 Yoshida, Yamaguchi 7538511, Japan; Kyungpook Natl Univ, Coll Vet Med, Dept Vet Internal Med, 80 Daehak Ro, Daegu 41566, North Gyeongsan, South Korea; Anim & Plant Quarantine Agcy, 177,Hyeoksin 8-Ro, Gimcheon Si 39660, Gyeongsangbuk D, South Korea; Guardian Co Ltd, 2794-127 Nishi Beppu Cho, Kagoshima 8900033, Japan; Airlangga Univ, Fac Vet Med, Campus C, Surabaya 60115, Indonesia | Rakib, Tofazzal/O-5344-2016; Rakib, Tofazzal Md/O-5344-2016; Islam, Md Shafiqul/GLS-1837-2022; Yunus, Muchammad/LEM-4072-2024; Tacharina, Martia/ADE-0651-2022 | 59281979900; 7402557377; 56118254400; 7407523067; 57190807204; 8357376100; 57207314733; 57219900903; 57221994194; 57191866506; 7005694765; 56230744900 | si.mamun@ymail.com;mtakagi@yamaguchi-u.ac.jp;kwolee@knu.ac.kr;bestofme@daum.net;okawa0117@guardian-vet.com;muchammad-y@fkh.unair.ac.id;titadlestari@fkh.unair.ac.id;martia.rt@fkh.unair.ac.id;s.pervin30@yahoo.com;rakibtofazza1367@gmail.com;yabu@vet.kagoshima-u.ac.jp;osam@vet.kagoshima-u.ac.jp; | ANIMALS | ANIMALS-BASEL | 2076-2615 | 12 | 8 | SCIE | AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES | 2022 | 3 | 8.7 | 0.2 | 2025-06-25 | 2 | 1 | bovine FOXP3 gene; regulatory T cell; allele frequency; Japanese black; Holstein Friesian; Hanwoo; Madura; cow; infertility | KOREAN NATIVE CATTLE; REPRODUCTIVE-PERFORMANCE; MUTANT ALLELE; PROFILE; DAIRY | allele frequency; bovine FOXP3 gene; cow; Hanwoo; Holstein Friesian; infertility; Japanese black; Madura; regulatory T cell | DNA; transcription factor FOXP3; animal experiment; animal model; Article; blood sampling; bovine; cattle breed; DNA extraction; female; genetic variability; genotype; infertility; nonhuman; pilot study; real time polymerase chain reaction; sampling; Sanger sequencing; single nucleotide polymorphism; X linked maternal variant | English | 2022 | 2022-04 | 10.3390/ani12081044 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Heat Stress during Summer Attenuates Expression of the Hypothalamic Kisspeptin, an Upstream Regulator of the Hypothalamic-Pituitary-Gonadal Axis, in Domestic Sows | Simple Summary The present study compared the expression of hypothalamic kisspeptin, an upstream regulator of the hypothalamic-pituitary-gonadal axis, in different seasons (summer and spring) to assess the effect of heat stress on reproductive performance in domestic sows. Sows in summer showed a decreased pregnancy rate and litter size and increased secretion of stress-related hormones than those in spring. Heat stress in summer lowered hypothalamic kisspeptin expression and reduced the activity of kisspeptin neurons. Due to the lowered expression of hypothalamic kisspeptin, the downstream reproductive hormones and the number of large ovarian follicles were reduced in summer. These findings may have significant implications in the fields of reproductive biology and the livestock industry for heat stress control. The release of reproductive hormones in the hypothalamic-pituitary-gonadal (HPG) axis is regulated by its upstream regulator, kisspeptin, and influenced by external stresses, including heat stress. Since the effect of heat stress (summer infertility) on hypothalamic kisspeptin expression in domestic sows is not yet understood, the present study attempted to identify changes in kisspeptin expression in different seasons (summer and spring). The high atmospheric temperature in summer decreased the pregnancy rate and litter size and increased stress-related hormones as a chronic stressor to domestic sows. The hypothalamic kisspeptin expression in summer was decreased regardless of the estrus phase and negatively correlated with atmospheric temperature, indicating that high temperature decreased kisspeptin. When the activity of hypothalamic kisspeptin neurons in the follicular phase was assessed using c-Fos staining, a decreased number of kisspeptin neurons coexpressing c-Fos was observed in domestic sows in summer. Accordingly, lower expression of kisspeptin induced decreased levels of HPG axis-related reproductive hormones, such as gonadotropins and estrogen, and fewer large ovarian follicles. In conclusion, the present study demonstrated that reduced kisspeptin expression and its neuronal activity in the hypothalamus under heat stress in summer induced downregulation of the HPG axis and caused summer infertility in domestic sows. | Kim, Hwan-Deuk; Kim, Young-Jong; Jang, Min; Bae, Seul-Gi; Yun, Sung-Ho; Lee, Mi-Ree; Seo, Yong-Ryul; Cho, Jae-Keun; Kim, Seung-Joon; Lee, Won-Jae | Kyungpook Natl Univ, Coll Vet Med, Daegu 41566, South Korea; Daegu Metropolitan City Inst Hlth & Environm, Dept Vet Res, Daegu 42183, South Korea; Kyungpook Natl Univ, Inst Equine Med, Daegu 41566, South Korea | 57219801684; 57203809959; 55341507600; 55418865900; 57201366734; 57962037200; 57962562500; 57963088700; 55718678200; 57205486455 | iamcyshd@knu.ac.kr; | ANIMALS | ANIMALS-BASEL | 2076-2615 | 12 | 21 | SCIE | AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES | 2022 | 3 | 8.7 | 2.44 | 2025-06-25 | 11 | 12 | kisspeptin; domestic sows; heat stress; summer infertility; c-Fos; hypothalamic-pituitary-gonadal axis | LUTEINIZING-HORMONE SECRETION; MESSENGER-RNA EXPRESSION; HYPOPHYSEAL PORTAL BLOOD; NEURON ACTIVATION; KISS1 GENE; C-FOS; GONADOTROPIN; REPRODUCTION; RECEPTOR; RELEASE | c-Fos; domestic sows; heat stress; hypothalamic–pituitary–gonadal axis; kisspeptin; summer infertility | estrogen; glyceraldehyde 3 phosphate dehydrogenase; gonadotropin; kisspeptin; animal experiment; animal model; animal tissue; Article; chemoluminescence; controlled study; enzyme linked immunosorbent assay; estrus cycle; follicular phase; heat stress; hypothalamus; hypothalamus hypophysis gonad system; immunofluorescence assay; infertility; nonhuman; ovary follicle; physiological stress; polyacrylamide gel electrophoresis; pregnancy; pregnancy rate; protein phosphorylation; radioimmunoprecipitation; sow (swine); summer; Western blotting | English | 2022 | 2022-11 | 10.3390/ani12212967 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Impact of the Deep Neuromuscular Block on Oncologic Quality of Laparoscopic Surgery in Obese Gastric Cancer Patients: A Randomized Clinical Trial | BACKGROUND: Obesity can hinder laparoscopic procedures and impede oncological safety during laparoscopic cancer surgery. Deep neuromuscular block (NMB) reportedly improves laparoscopic surgical conditions, but its oncological benefits are unclear. We aimed to evaluate whether deep NMB improves the oncologic quality of laparoscopic cancer surgery in obese patients. STUDY DESIGN: We conducted a double-blinded, parallel-group, randomized, phase 3 trial at 9 institutions in Korea. Clinical stage I and II gastric cancer patients with a BMI at or above 25 kg m (-2) were eligible and randomized 1:1 ratio to the deep or moderate NMB groups, with continuous infusion of rocuronium (0.5-1.0 and 0.1-0.5 mg kg (-1) h (-1), respectively). The primary endpoint was the number of retrieved lymph nodes (LNs). The secondary endpoints included the surgeon's surgical rating score (SRS) and interrupted events. RESULTS: Between August 2017 and July 2020, 196 patients were enrolled. Fifteen patients were excluded, and 181 patients were finally included in the study. There was no significant difference in the number of retrieved LNs between the deep (N = 88) and moderate NMB groups (N = 93; 44.6 +/- 17.5 vs 41.5 +/- 16.9, p = 0.239). However, deep NMB enabled retrieving more LNs in patients with a BMI at or above 28 kg/m(2) than moderate NMB (49.2 +/- 18.6 vs 39.2 +/- 13.3, p = 0.026). Interrupted events during surgery were lower in the deep NMB group than in the moderate NMB group (21.6% vs 36.6%; p = 0.034). The SRS was not influenced by NMB depth. CONCLUSION: Deep NMB provides potential oncologic benefits by retrieving more LNs in patients with BMI at or above 28 kg/m(2) during laparoscopic gastrectomy. (C) 2022 by the American College of Surgeons. Published by Wolters Kluwer Health, Inc. All rights reserved. | Park, Shin-Hoo; Huh, Hyub; Choi, Sung Il; Kim, Jong-Han; Jang, You-Jin; Park, Joong-Min; Kwon, Oh Kyoung; Jung, Mi Ran; Jeong, Oh; Lee, Chang Min; Min, Jae Seok; Kim, Jin-Jo; An, Liang; Yang, Kyung Sook; Park, Sungsoo; Lee, Il Ok | Korea Univ, Dept Surg, Div Foregut Surg, Coll Med, Seoul, South Korea; Korea Univ, Div Foregut Surg, Anam Hosp, Seoul, South Korea; Kyung Hee Univ Hosp Gangdong, Dept Anesthesiol & Pain Med Huh, Seoul, South Korea; Kyung Hee Univ Hosp Gangdong, Dept Surg, Seoul, South Korea; Korea Univ, Dept Surg, Div Foregut Surg, Guro Hosp, Seoul, South Korea; Chung Ang Univ, Dept Surg, Coll Med, Seoul, South Korea; Kyungpook Natl Univ, Sch Med, Dept Surg, Daegu, South Korea; Chonnam Natl Univ, Dept Surg, Med Sch, Jeollanam Do, South Korea; Korea Univ, Div Foregut Surg, Ansan Hosp, Seoul, South Korea; Dongnam Inst Radiol & Med Sci, Canc Ctr, Dept Surg, Busan, South Korea; Catholic Univ Korea, Dept Surg, Incheon St Marys Hosp, Coll Med, Incheon, South Korea; Zhejiang Univ, Shaoxing Hosp, Sch Med, Shaoxing, Peoples R China; Korea Univ, Dept Biostat, Coll Med, Seoul, South Korea; Korea Univ, Dept Anesthesiol & Pain Med, Guro Hosp, Seoul, South Korea | Lee, Chang/AAA-8912-2021; Park, Sun-Young/B-4634-2014; Kim, Jonghan/AAH-9562-2021; Min, Jae-Seok/ABG-5529-2022; 박, 중민/GMW-4506-2022; Huh, Hyub/HPE-2938-2023 | 57188744111; 57192182870; 25959932300; 55081512500; 35573659700; 14030287200; 26536109900; 39861699600; 26428191900; 56901528900; 37031730000; 36013396100; 57204105777; 35270603000; 57187346700; 7404441699; 56599265100; 23670633900; 26325931400; 58034860300; 57225826955; 36126257100; 58251278200; 57194619410; 57205138808; 55615093400; 7404036103 | kugspss@korea.ac.kr; | JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS | J AM COLL SURGEONS | 1072-7515 | 1879-1190 | 234 | 3 | SCIE | SURGERY | 2022 | 5.2 | 8.7 | 1.28 | 2025-06-25 | 7 | 8 | LYMPH-NODE DISSECTION; IMPROVES SURGICAL CONDITIONS; BILLROTH II RECONSTRUCTION; ROUX-EN-Y; CURATIVE RESECTION; EXTENDED LYMPHADENECTOMY; DISTAL GASTRECTOMY; STAGE MIGRATION; SURVIVAL; NUMBER | Humans; Laparoscopy; Neuromuscular Blockade; Obesity; Rocuronium; Stomach Neoplasms; rocuronium; sugammadex; rocuronium; abdominal bleeding; adult; aged; Article; artificial pneumoperitoneum; body mass; cancer staging; cholecystectomy; clinical outcome; continuous infusion; controlled study; distal Roux-en Y gastric bypass; double blind procedure; endocrine disease; female; gastrectomy Billroth II; health care quality; heart disease; human; kidney disease; laparoscopic distal gastrectomy; liver disease; lung disease; lymph node; lymph node dissection; major clinical study; male; middle aged; multicenter study; neuromuscular blocking; obese patient; obesity; operative blood loss; pancreatitis; parallel design; phase 3 clinical trial; postoperative complication; postoperative ileus; postoperative nausea and vomiting; randomized controlled trial; scoring system; stenosis; stomach adenocarcinoma; urogenital tract disease; wound complication; clinical trial; complication; laparoscopy; obesity; procedures; stomach tumor | English | 2022 | 2022-03 | 10.1097/xcs.0000000000000061 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Influences of Dog Attachment and Dog Walking on Reducing Loneliness during the COVID-19 Pandemic in Korea | Simple Summary Even before the emergence of COVID-19, animal companionship was gaining popularity in Korea as a strategy to reduce loneliness and isolation caused by a rapidly aging population and an increase in one-person households. Many dog owners have experienced the emotional and physical benefits that dog ownership provides during the COVID-19 period. This study examined the relationship between dog attachment, dog walking and loneliness during times of widespread isolation. An online, cross-sectional survey for dog owners was conducted in the fall of 2020. Voluntary participants responded to questionnaires about their relationship with dogs, dog attachment, and dog walking during COVID-19. Our study suggests that dog walking has no direct effect on reducing loneliness but the relationship between dog walking and loneliness might be mediated by attachment. Due to the strict social distancing guidelines during the pandemic, dog walking would not allow opportunities for conversation with other people, but it seems that spending time outside with dogs strengthens the bonds between humans and companion dogs, alleviating loneliness. The COVID-19 pandemic has changed people's lives and increased their vulnerability to physical and mental health hazards. While Korea has avoided nationwide lockdown measures since the COVID-19 outbreak, the prolonged restrictions and social isolation measures have resulted in detrimental psychological effects, such as increased anxiety, boredom, and loneliness. The present study investigated dog attachment and changes in dog walking during the COVID-19 pandemic and the effects of dog attachment and dog walking on the loneliness of Korean dog owners. An online, cross-sectional survey was conducted in the fall of 2021 in which 249 dog owners responded to questionnaires that asked questions about dog attachment, their perception of dog walking, and their feelings of loneliness during the COVID-19 pandemic. Most dog owners responded that they spent more time with their dogs and developed a stronger bond with them during the pandemic. Additionally, respondents stated that they walked their dogs more often than they did before COVID-19 and that their dogs aided in reducing loneliness. We found that dog walking directly affected attachment and indirectly influenced the loneliness of dog owners. Further research is required to determine how dog walking impacts positive psychological effects and promote dog walking. | Lee, Hyung-Sook; Song, Jin-Gyeoung; Lee, Jeong-Yeon | Kyungpook Natl Univ, Dept Landscape Architecture, Daegu 41566, South Korea | Lee, Hyung/HGB-2587-2022; Jeong Yeon, Lee/NFT-1014-2025 | 35210890100; 57449056700; 57449281300 | soolee@knu.ac.kr;luvpopconi@knu.ac.kr;hyo04031@knu.ac.kr; | ANIMALS | ANIMALS-BASEL | 2076-2615 | 12 | 4 | SCIE | AGRICULTURE, DAIRY & ANIMAL SCIENCE;VETERINARY SCIENCES | 2022 | 3 | 8.7 | 2.65 | 2025-06-25 | 12 | 14 | companion animals; dog walking; dog attachment; loneliness; COVID-19 | OWNERSHIP; BEHAVIOR | Companion animals; COVID-19; Dog attachment; Dog walking; Loneliness | adult; animal experiment; animal model; anxiety; Article; behavior; boredom; child; coronavirus disease 2019; cross-sectional study; dog; education; female; health behavior; health care survey; information processing; loneliness; male; mental health; nonhuman; outcome assessment; pandemic; physical activity; psychological well-being; psychometry; questionnaire; social distance; social isolation; walking | English | 2022 | 2022-02 | 10.3390/ani12040483 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Psychiatry curriculum: How does it affect medical students' attitude toward psychiatry? | The purpose of this study was to identify the attitude of South Korean medical school students towards psychiatry and to analyze how lectures and clinical clerkship in psychiatry effect changes in students' attitudes. A total of 100 medical school students were recruited as subjects in the study, 86 of whom completed psychiatry lectures and underwent psychiatry clerkship from March 2015 to August 2018. The participating students completed a survey, which included demographic data, specialty choice, and attitude towards psychiatry. Subjects who completed lectures and clinical clerkship in psychiatry showed positive changes in attitude towards psychiatry in the following items: "Among mental health professionals, psychiatrists have the most authority and influence" (p = 0.002), "Psychiatrists frequently abuse their legal power to hospitalize patients against their will" (p < 0.001), and "Many people who could not obtain a residency position in other specialties eventually enter psychiatry" (p = 0.028). However, negative changes in attitude towards psychiatry were shown in the following item: "On average, psychiatrists make as much money as most other doctors" (p = 0.008). Also, specific medical school factors scored positively overall. Although it was not statistically significant, the questionnaire item "During my psychiatry rotation, psychiatry residents were good role models" was more positively observed by students after completion of psychiatry lectures and clerkship. After each curriculum, certain attitudes toward psychiatry were positively changed, but others either remained unchanged or were negatively changed. By using these study results to revise the current curriculum, medical school students' attitude towards psychiatry can be improved. | Lee, Hye Jeong; Jang, Hyeok Jun; Jang, Seoyoung; Bong, Su Hyun; Won, Geun Hui; Yoon, Seoyoung; Choi, Tae Young | Daegu Catholic Univ, Sch Med, Dept Psychiat, 33,Duryugongwon ro 17-gil, Daegu 42472, South Korea; Seoul Reg Mil Manpower Adm, Seoul, South Korea; Kyungpook Natl Univ, Sch Med, Daegu, South Korea; Seoul Natl Univ Hosp, Dept Neuropsychiatry, Seoul, South Korea | 57280746700; 57343392900; 57343109500; 57219670611; 57199505446; 56449806300; 55694739600 | tyoungchoi@cu.ac.kr; | ASIAN JOURNAL OF PSYCHIATRY | ASIAN J PSYCHIATR | 1876-2018 | 1876-2026 | 67 | SCIE | PSYCHIATRY | 2022 | 9.5 | 8.7 | 0.59 | 2025-06-25 | 6 | 5 | Attitude; Psychiatry; Curriculum; Clinical clerkship; Republic of Korea | PRIMARY-CARE; CHOICES | Attitude; Clinical clerkship; Curriculum; Psychiatry; Republic of Korea | Attitude; Attitude of Health Personnel; Career Choice; Curriculum; Humans; Psychiatry; Students, Medical; Surveys and Questionnaires; adult; article; clinical education; controlled study; curriculum; demography; female; human; major clinical study; male; medical student; mental health care personnel; money; psychiatrist; psychiatry; questionnaire; resident; rotation; South Korea; attitude; curriculum; decision making; education; health personnel attitude | English | 2022 | 2022-01 | 10.1016/j.ajp.2021.102919 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Semi-enclosed paper sensor for highly sensitive and selective detection of proline | In the current study, we have utilized semi-enclosed, leak-proof, micmfluidic paper-based analytical devices (mu PAD's) modified with isatin conjugated chitosan as specific colorimetric reagent for the detection of proline. Praline is one of the globally accepted stress biomarker in plants and also one of the prominent amino acid present in wine and some processed food. Quantification of proline is regularly required in agriculture field, food and wine industries. Specific interaction of isatin with proline, uniform film forming ability of chitosan which results in uniform coloration and the presence of leak-proof layer which prevent the diffusion of colorimetric reagent deeper resulted in enhancement of color signal intensity at the reaction zone were utilized. Further, the images of the mu PAD's were captured using smartphone with 3D printed imaging box which houses smartphone and mu PAD's. This platform utilizes smartphone flash for uniform illumination and ensures constant positioning of mu PAD's to capture images. This greatly enhances the sensitivity and accuracy of our platform. Compared to previously published highly sensitive multi-layer, paper-based platform for detection of proline, current method has enhanced detection range (similar to 7 fold) and has comparable limit of detection of 23.75 mu M. Moreover, the developed mu PAD's platform has reduced optimum reaction temperature and time compared to previous work. The developed paper based platform was utilized for evaluation of proline content in young Arabidopsis plants which are subjected to water stress for 5 days. The devised paper-based methods have the potential to be applicable for the on-site evaluation of various stresses in plants. | Santhosh, Mallesh; Park, Tusan | Kyungpook Natl Univ, Smart Agr Innovat Ctr, Daegu, South Korea; Kyungpook Natl Univ, Bioind Machinery Engn, Daegu, South Korea | Park, Tusan/C-2057-2012 | 56533710200; 57202780408 | tusan.park@knu.ac.kr; | ANALYTICA CHIMICA ACTA | ANAL CHIM ACTA | 0003-2670 | 1873-4324 | 1231 | SCIE | CHEMISTRY, ANALYTICAL | 2022 | 6.2 | 8.7 | 0.22 | 2025-06-25 | 3 | 3 | VALIDATION; DEVICE; HONEY | Arabidopsis; Chitosan; Colorimetry; Indicators and Reagents; Isatin; Lab-On-A-Chip Devices; Paper; Proline; 3D printers; Analytic equipment; Chemical reactions; Chitosan; Color; Colorimetric analysis; Colorimetry; Wine; amino acid; chitosan; isatin; proline; chitosan; dyes, reagents, indicators, markers and buffers; isatin; proline; 'current; Amino-acids; Colorimetric reagents; Food industries; Highly sensitive detections; Leak proofs; Paper-based analytical devices; Selective detection; Smart phones; Stress biomarkers; amino acid analysis; Arabidopsis; Article; controlled study; diffusion; Fourier transform infrared spectroscopy; illumination; limit of detection; microfluidics; nonhuman; reaction temperature; reaction time; three-dimensional imaging; ultraviolet visible spectroscopy; water stress; wine industry; Arabidopsis; chemistry; colorimetry; lab on a chip; paper; procedures; Smartphones | English | 2022 | 2022-10-23 | 10.1016/j.aca.2022.340399 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | The Diversity Patterns of Rare to Abundant Microbial Eukaryotes Across a Broad Range of Salinities in a Solar Saltern | Solar salterns are excellent artificial systems for examining species diversity and succession along salinity gradients. Here, the eukaryotic community in surface water of a Korean solar saltern (30 to 380 practical salinity units) was investigated from April 2019 to October 2020 using Illumina sequencing targeting the V4 and V9 regions of 18S rDNA. A total of 926 operational taxonomic units (OTUs) and 1,999 OTUs were obtained with the V4 and V9 regions, respectively. Notably, most of the OTUs were microbial eukaryotes, and the high-abundance groups (> 5% relative abundance (RA), Alveolata, Stramenopila, Archaeplastida, and Opisthokonta) usually accounted for > 90% of the total cumulative read counts and > 80% of all OTUs. Moreover, the high-abundance Alveolata (larger forms) and Stramenopila (smaller forms) groups displayed a significant inverse relationship, probably due to predator-prey interactions. Most of the low-abundance (0.1-5% RA) and rare (< 0.1% RA) groups remained small portion during the field surveys. Taxonomic novelty (at < 90% sequence identity) was high in the Amoebozoa, Cryptista, Haptista, Rhizaria, and Stramenopila groups (69.8% of all novel OTUs), suggesting the presence of a large number of hidden species in hypersaline environments. Remarkably, the high-abundance groups had little overlap with the other groups, implying the weakness of rare-to-prevalent community dynamics. The low-abundance Discoba group alone temporarily became the high-abundance group, suggesting that it is an opportunistic group. Overall, the composition and diversity of the eukaryotic community in hypersaline environments may be persistently stabilized, despite diverse disturbance events. | Lee, Hyeon Been; Jeong, Dong Hyuk; Cho, Byung Cheol; Park, Jong Soo | Kyungpook Natl Univ, Dept Oceanog, Daegu 41566, South Korea; Seoul Natl Univ, Sch Earth & Environm Sci, Seoul 08826, South Korea; Kunsan Natl Univ, Saemangeum Environm Res Ctr, Kunsan 54150, South Korea | Park, Jong/AAJ-3576-2020 | 57337671100; 57338712200; 7401747485; 23486274100 | jongsoopark@knu.ac.kr; | MICROBIAL ECOLOGY | MICROB ECOL | 0095-3628 | 1432-184X | 84 | 4 | SCIE | ECOLOGY;MARINE & FRESHWATER BIOLOGY;MICROBIOLOGY | 2022 | 3.6 | 8.7 | 1.37 | 2025-06-25 | 11 | 12 | Microbial eukaryotic diversity; Hypersaline ecosystems; Solar saltern; Salinity gradients; Operational taxonomic units | SALINARUM NOV SPEC.; RIBOSOMAL-RNA GENE; N. SP.; HETEROTROPHIC PROTISTS; MOLECULAR PHYLOGENY; DEEP-SEA; HYPERSALINE; CILIOPHORA; MARINE; MORPHOLOGY | Hypersaline ecosystems; Microbial eukaryotic diversity; Operational taxonomic units; Salinity gradients; Solar saltern | Alveolata; Biodiversity; DNA, Ribosomal; Eukaryota; Rhizaria; Salinity; ribosome DNA; Alveolata; biodiversity; eukaryote; genetics; Rhizaria; salinity | English | 2022 | 2022-11 | 10.1007/s00248-021-01918-1 | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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