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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | ○ | Article | Alpha-1 antitrypsin inhibits formaldehyde-induced apoptosis of human peritoneal mesothelial cells | Background: The alpha-1 antitrypsin (AAT) protein has an important role in the anti-inflammatory and apoptotic response. AAT inhibits not only serine proteases but also cysteine and aspartic proteases. Apoptosis results from the sequential activation of cysteine proteases of the caspase family. This study aimed to evaluate the effect of AAT on formaldehyde-induced apoptosis of human peritoneal mesothelial cells (HPMCs). Methods: HPMCs were cultured and treated with formaldehyde (250 mu M) to induce apoptosis. In the AAT group, the cultured HPMCs were pretreated with AAT (2 mg/mL) for 1 h before formaldehyde treatment. We used 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays to determine cell viability, and flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assays to detect apoptosis. The MTT assays were used to find optimal concentrations of formaldehyde and AAT. We measured caspase-3 activity and used Western blotting to estimate Bcl-2 and Bad expression. Results: Flow cytometry and TUNEL assays revealed that formaldehyde exposure significantly increased apoptosis compared with the control treatment, but pretreatment with AAT significantly inhibited this effect. Compared with the control, caspase-3 activity was significantly increased and the ratio of Bcl-2 to Bad expression significantly decreased following treatment with formaldehyde. However, caspase-3 activity was significantly lower and the Bcl-2 to Bad expression ratio higher in the AAT group than in the formaldehyde-only group. Conclusion: AAT inhibits formaldehyde-induced apoptosis of HPMCs via a caspase-mediated pathway. These data support a potential use for AAT as a therapeutic agent for the inhibition of peritoneal cell apoptosis during peritoneal dialysis. | Lim, Jeong-Hoon; Park, Sang Mi; Yook, Ju-Min; Ahn, Ji-Sun; Choi, Soon-Youn; Oh, Se-Hyun; Jung, Hee-Yeon; Choi, Ji-Young; Cho, Jang-Hee; Park, Sun-Hee; Kim, Yong-Lim; Kim, Chan-Duck | Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Div Nephrol,Dept Internal Med, Daegu, South Korea | Kim, Yong-Lim/AGK-3172-2022; Lim, Jeong-Hoon/ABE-6003-2020; Park, Sun-Hee/LMN-0033-2024; Cho, Jang-hee/ABD-3534-2020; Choi, Soon Youn/AHC-8865-2022 | 55360244300; 57201988114; 35110084800; 57191631694; 15845172400; 56053033900; 57196396467; 7501393222; 7403536291; 7501831741; 55633533600; 8558530700 | drcdkim@knu.ac.kr; | PERITONEAL DIALYSIS INTERNATIONAL | PERITON DIALYSIS INT | 0896-8608 | 1718-4304 | 40 | 2 | SCIE | UROLOGY & NEPHROLOGY | 2020 | 1.756 | 80.3 | 0.19 | 2025-06-25 | 5 | 4 | Alpha-1 antitrypsin; apoptosis; caspase-3 activity; human peritoneal mesothelial cells; peritoneal dialysis | GLUCOSE DEGRADATION-PRODUCTS; GLYCATION END-PRODUCTS; DIALYSIS SOLUTIONS; FLUID; INFLAMMATION; CASPASE-3 | Alpha-1 antitrypsin; apoptosis; caspase-3 activity; human peritoneal mesothelial cells; peritoneal dialysis | alpha 1-Antitrypsin; Apoptosis; bcl-Associated Death Protein; Caspase 3; Cell Culture Techniques; Cell Survival; Epithelial Cells; Formaldehyde; Humans; Peritoneal Dialysis; Peritoneum; Proto-Oncogene Proteins c-bcl-2; alpha 1 antitrypsin; alpha 1 antitrypsin concentrate; caspase 3; formaldehyde; protein BAD; protein bcl 2; alpha 1 antitrypsin; BAD protein, human; BCL2 protein, human; caspase 3; formaldehyde; protein BAD; protein bcl 2; SERPINA1 protein, human; apoptosis; Article; cell structure; cell viability; controlled study; enzyme activity; flow cytometry; human; human cell; human tissue; mesothelium cell; MTT assay; peritoneal dialysis; peritoneum cell; protein expression; TUNEL assay; Western blotting; apoptosis; cell culture technique; cell survival; drug effect; epithelium cell; metabolism; pathology; peritoneum | English | 2020 | 2020-03 | 10.1177/0896860819887288 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Deletion of Peroxiredoxin II Inhibits the Growth of Mouse Primary Mesenchymal Stem Cells Through Induction of the G0/G1 Cell-cycle Arrest and Activation of AKT/GSK3β/β-Catenin Signaling | Background/Aim: Dermal mesenchymal stem cells (DMSCs) are pluripotent stem cells found in the skin which maintain the thickness of the dermal layer and participate in skin wound healing. Materials and Methods: The MTT assay was performed to detect cell proliferation and cell-cycle progression and cell-surface markers were assessed by flow cytometry. The levels of proteins in related signaling pathways were detected by western blotting assay and the translocation of beta-catenin into the nucleus were detected by immunofluorescence. Red oil O staining was performed to examine the differentiational ability of DMSCs. Results: Knockout of PRDX2 inhibited DMSC cell growth, and cell-cycle arrest at G(0)/G(1) phase; p16, p21 and cyclin D1 expression levels in Prdx2 knockout DMSCs were significantly increased. Furthermore, AKT phosphorylation were significantly increased in Prdx2 knockout DMSCs, GSK3 beta activity were inhibited, result in beta-Catenin accumulated in the nucleus. Conclusion: In conclusion, these results demonstrated that PRDX2 plays a pivotal role in regulating the proliferation of DMSCs, and this is closely related to the AKT/glycogen synthase kinase 3 beta/beta-catenin signaling pathway. | Han, Ying-Hao; Jin, Mei-Hua; Jin, Ying-Hua; Yu, Nan-Nan; Liu, Jun; Zhang, Yong-Qing; Cui, Yu-Dong; Wang, Ai-Guo; Lee, Dong-Seok; Kim, Sun-Uk; Kim, Ji-Su; Kwon, Taeho; Sun, Hu-Nan | Heilongjiang Bayi Agr Univ, Coll Life Sci & Technol, Daqing 163319, Heilongjiang, Peoples R China; Heilongjiang Bayi Agr Univ, Coll Life Sci & Technol, Lib & Informat Ctr, Daqing, Heilongjiang, Peoples R China; Dalian Med Univ, Lab Anim Ctr, Dalian, Peoples R China; Kyungpook Natl Univ, Sch Life Sci, BK21 Plus Project, KNU Creat BioRes Grp, Daegu, South Korea; Korea Res Inst Biosci & Biotechnol, Futurist Anim Resource & Res Ctr, Cheongju, South Korea; Korea Res Inst Biosci & Biotechnol, Primate Resources Ctr, 351-33 Neongme Gil, Jeongeup Si 56216, Jeonbuk, South Korea | Han, Ying-Hao/IWU-6583-2023; Kwon, Taeho/JLL-1095-2023; Jin, Meihua/AAE-8526-2020; Kim, Sunuk/KJM-5211-2024; jin, Yinghua/T-7461-2019; Han, hao/IWU-6583-2023 | 7404096642; 57740660100; 8610761600; 57212654215; 59649419200; 57212661350; 24342913900; 57190730121; 57210068061; 8278891100; 39461534900; 57202850676; 8603267900 | hyhbynd@163.com;kwon@kribb.re.kr;sunhunan76@163.com; | IN VIVO | IN VIVO | 0258-851X | 1791-7549 | 34 | 1 | SCIE | MEDICINE, RESEARCH & EXPERIMENTAL | 2020 | 2.155 | 80.4 | 0.58 | 2025-06-25 | 13 | 13 | Peroxiredoxin II; cell cycle; mesenchymal stem cells; glycogen synthase kinase 3 beta/beta-catenin signaling | SYNTHASE KINASE 3; REACTIVE OXYGEN; IN-VITRO; CATENIN; DIFFERENTIATION; KNOCKDOWN; EXPANSION | Cell cycle; Glycogen synthase kinase 3 beta/β-catenin signaling; Mesenchymal stem cells; Peroxiredoxin II | Animals; Apoptosis; beta Catenin; Cell Cycle Checkpoints; Cell Line; Cell Proliferation; G1 Phase; Glycogen Synthase Kinase 3 beta; Mesenchymal Stem Cells; Mice; Mice, Knockout; Peroxiredoxins; Phosphorylation; Proto-Oncogene Proteins c-akt; Resting Phase, Cell Cycle; Signal Transduction; beta catenin; cell surface marker; cyclin D1; cycline; glycogen synthase kinase 3beta; peroxiredoxin 2; protein kinase B; protein p16; protein p21; STAT3 protein; beta catenin; glycogen synthase kinase 3beta; peroxiredoxin; protein kinase B; Akt signaling; animal cell; Article; cell cycle progression; cell differentiation; cell growth; cell isolation; cell proliferation; enzyme activation; flow cytometry; G1 phase cell cycle checkpoint; immunofluorescence; mesenchymal stem cell; mouse; MTT assay; newborn; nonhuman; pluripotent stem cell; protein phosphorylation; upregulation; Western blotting; animal; apoptosis; cell cycle checkpoint; cell cycle G0 phase; cell cycle G1 phase; cell line; genetics; knockout mouse; mesenchymal stem cell; pathology; phosphorylation; signal transduction | English | 2020 | 2020 (JAN-FEB) | 10.21873/invivo.11754 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Glycerols and fatty acids isolated from Micractinium sp. KSF0031 | From the collected extract from King Sejong Antarctic Station, strain Micractinium sp. KSF0031, led to the isolation of one new monoacyldigalactosyl glycerol (1) and seven known compounds (2-8). Their chemical structures were established using extensive spectroscopic techniques, including 1D, 2D-NMR, and MS, and compared with the published data. To the best of our knowledge, this is the first report to investigate the secondary metabolites from genus Micractinium. The monoacyldigalactosyl glycerol in Micractinium could serve as its chemotaxonomic markers. | Huynh Nguyen Khanh Tran; Youn, Ui Joung; Kim, Jeong Ah; Chae, Hyunsik; Kim, Sanghee; Min, Byung Sun | Drug Daegu Catholic Univ, Coll Pharm, Gyeongbuk 38430, South Korea; Korea Polar Res Inst, Div Life Sci, Incheon 21990, South Korea; Kyungpook Natl Univ, Coll Pharm, Res Inst Pharmaceut Sci, Daegu 41566, South Korea | 57205206862; 16417618600; 24722570900; 55785278500; 57208574704; 55663638200 | sangheekim@kopri.re.kr;bsmin@cu.ac.kr; | BIOCHEMICAL SYSTEMATICS AND ECOLOGY | BIOCHEM SYST ECOL | 0305-1978 | 1873-2925 | 89 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;ECOLOGY;EVOLUTIONARY BIOLOGY | 2020 | 1.381 | 80.4 | 0.09 | 2025-06-25 | 2 | 2 | Chlorellaceae; Micractinium sp. KSF0031; Glycerol | Chlorellaceae; Glycerol; Micractinium sp. KSF0031 | Chlorellaceae; Micractinium; biomarker; fatty acid; green alga; mass spectrometry; plant extract; secondary metabolite | English | 2020 | 2020-04 | 10.1016/j.bse.2019.104000 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Picrasma quassioides Extract Elevates the Cervical Cancer Cell Apoptosis Through ROS-Mitochondrial Axis Activated p38 MAPK Signaling Pathway | Background/Aim: Picrasma quassioides (P. quassioides) is used in traditional Asian medicine widely for the treatment of anemopyretic cold, eczema, nausea, loss of appetite, diabetes mellitus, hypertension etc. In this study we aimed to understand the effect of P. quassioides ethanol extract on SiHa cervical cancer cell apoptosis. Materials and Methods: The P. quassioides extract-induced apoptosis was analyzed using the MTT assay, fluorescence microscopy, flow cytometry and western blotting. Results: P. quassioides extract induced cellular apoptosis by increasing the accumulation of cellular and mitochondrial reactive oxygen species (ROS) levels and inhibiting ATP synthesis. Pretreatment with N-Acetylcysteine (NAC), a classic antioxidant, decreased the intracellular ROS production and inhibited apoptosis. In addition, the P38 MAPK signaling pathway is a key in the apoptosis of SiHa cells induced by the P. quassioides extract. Conclusion: The P. quassioides extract exerts its anti-cancer properties on SiHa cells through ROS-mitochondria axis and P38 MAPK signaling. Our data provide a new insight for P. quassioides as a therapeutic strategy for cervical cancer treatment. | Gong, Yi-Xi; Liu, Yue; Jin, Ying-Hua; Jin, Mei-Hua; Han, Ying-Hao; Li, Jing; Shen, Gui-Nan; Xie, Dan-Ping; Ren, Chen-Xi; Yu, Li-Yun; Lee, Dong-Seok; Kim, Ji-Su; Jo, Yu-Jin; Kwon, Jeongwoo; Lee, Jaihyung; Park, Yang Ho; Kwon, Taeho; Cui, Yu-Dong; Sun, Hu-Nan | Heilongjiang Bayi Agr Univ, Coll Life Sci & Technol, Daqing 163319, Heilongjiang, Peoples R China; Heilongjiang Bayi Agr Univ, Lib & Informat Ctr, Daqing, Peoples R China; Kyungpook Natl Univ, Sch Life Sci, KNU Creat BioRes Grp BK21 Plus Project, Daegu, South Korea; Korea Res Inst Biosci & Biotechnol KRIBB, Primate Resources Ctr, 351-33 Neongme Gil, Jeongeup Si 56216, Jeonbuk, South Korea; Chungbuk Natonal Univ, Dept Anim Sci, Cheongju, South Korea; Haeam Convalescence Hosp, Gyeonggi, South Korea; Pk Yang Ho BRM Inst, Seoul, South Korea | Kwon, Taeho/JLL-1095-2023; jin, Yinghua/T-7461-2019; Jin, Meihua/AAE-8526-2020 | 57209617864; 57208631142; 8610761600; 57740660100; 7404096642; 59793458600; 36193289800; 57209616350; 57217585371; 8604011000; 57210068061; 39461534900; 56321088600; 56372575200; 57217595872; 59063842600; 57202850676; 24342913900; 8603267900 | kwon@kribb.re.kr;cuiyudong@126.com;sunhunan76@163.com; | IN VIVO | IN VIVO | 0258-851X | 1791-7549 | 34 | 4 | SCIE | MEDICINE, RESEARCH & EXPERIMENTAL | 2020 | 2.155 | 80.4 | 0.71 | 2025-06-25 | 20 | 17 | Picrasma quassioides; cervical cancer; apoptosis; ROS; mitochondria | DEPENDENT APOPTOSIS; ALKALOIDS; STRESS; GROWTH | Apoptosis; Female; Humans; Membrane Potential, Mitochondrial; Mitochondria; p38 Mitogen-Activated Protein Kinases; Picrasma; Reactive Oxygen Species; Signal Transduction; Uterine Cervical Neoplasms; acetylcysteine; adenosine triphosphate; alcohol; antineoplastic agent; caspase 3; caspase 9; mitogen activated protein kinase; mitogen activated protein kinase p38; Picrasma quassioides extract; plant extract; protein Bax; protein bcl xl; reactive oxygen metabolite; stress activated protein kinase; unclassified drug; mitogen activated protein kinase p38; reactive oxygen metabolite; antiapoptosis; antineoplastic activity; apoptosis; Article; cell migration; cell survival; cell viability; cervical cancer cell line; controlled study; down regulation; flow cytometry; fluorescence microscopy; human; human cell; MAPK signaling; membrane permeability; membrane potential; mitochondrion; MTT assay; Picrasma; Picrasma quassioides; protein expression; SiHa cell line; synthesis; upregulation; Western blotting; apoptosis; female; genetics; metabolism; mitochondrial membrane potential; mitochondrion; signal transduction; uterine cervix tumor | English | 2020 | 2020 (JUL-AUG) | 10.21873/invivo.11977 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Prognostic Impact of the Neoadjuvant Rectal Score as Compared With the Tumor Regression Grade and Yield Pathologic TNM Stage in Patients With Locally Advanced Rectal Cancer After Neoadjuvant Chemoradiotherapy | Background/Aim: The present study compared the prognostic value of the yield pathologic (yp) stage, tumor regression grade (TRG), and neoadjuvant rectal (NAR) score in patients with locally advanced rectal cancer (LARC) who received neoadjuvant chemoradiotherapy (nCRT). Patients and Methods: For the assessment of tumor regression, the Dworak grading system was used. The NAR score was calculated using the following equation: (5ypN-3[cT-ypT]+12)(2) divided by 9.61. Results: In univariate analysis, the NAR score and ypTNM stage were significantly associated with DFS [hazard ratio (HR)=2.514, p<0.001 and HR=3.200, p<0.001] and OS (HR=2.292, p=0.001 and HR=2.859, p<0.001), whereas the TRG was significantly associated with only DFS (HR=2.008, p=0.017). In multivariate analysis, the ypTNM stage was the only independent prognostic factor for DFS (HR=3.796, p<0.001) and OS (HR=3.591, p=0.0034). Conclusion: Only the ypTNM stage was significantly associated with survival outcomes in multivariate analysis, suggesting that it is the most powerful prognostic factor of nCRT in patients with LARC. | Baek, Jin Ho; Baek, Dong Won; Kang, Byung Woog; Kim, Hye Jin; Park, Su Yeon; Park, Jun Seok; Choi, Gyu Seog; Kim, Jong Gwang | Kyungpook Natl Univ, Canc Res Inst, Dept Oncol Hematol, Chilgok Hosp,Sch Med, 807 Hogukno, Daegu 41404, South Korea; Kyungpook Natl Univ, Dept Surg, Chilgok Hosp, Sch Med, Daegu, South Korea | Park, Junsu/IQU-2241-2023; Kim, Hye/W-1059-2019 | 57217075847; 57191874272; 28567838500; 57204567554; 57203275898; 35226761100; 8058759100; 59501049300 | jkk21c@knu.ac.kr; | IN VIVO | IN VIVO | 0258-851X | 1791-7549 | 34 | 4 | SCIE | MEDICINE, RESEARCH & EXPERIMENTAL | 2020 | 2.155 | 80.4 | 0.65 | 2025-06-25 | 13 | 13 | Rectal neoplasms; neoadjuvant therapy; neoplasm staging; prognosis | ADJUVANT CHEMOTHERAPY USE; PREOPERATIVE CHEMORADIOTHERAPY; POSTOPERATIVE CHEMOTHERAPY; FREE SURVIVAL; OPEN-LABEL; OXALIPLATIN; FLUOROURACIL; METAANALYSIS; MULTICENTER; RECURRENCE | Neoadjuvant therapy; Neoplasm staging; Prognosis; Rectal neoplasms | Chemoradiotherapy; Disease-Free Survival; Humans; Neoadjuvant Therapy; Neoplasm Staging; Prognosis; Rectal Neoplasms; Retrospective Studies; Treatment Outcome; capecitabine; fluorouracil; folinic acid; adjuvant chemoradiotherapy; adult; advanced cancer; aged; Article; cancer grading; cancer prognosis; cancer staging; cancer survival; comparative study; controlled study; disease free survival; dworak grading system; female; human; long term survival; major clinical study; male; neoadjuvant rectal score; overall survival; prognostic assessment; rectum cancer; retrospective study; scoring system; treatment outcome; tumor regression; cancer staging; chemoradiotherapy; neoadjuvant therapy; pathology; prognosis; rectum tumor | English | 2020 | 2020 (JUL-AUG) | 10.21873/invivo.11997 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Quantitative Assessment of Interim PET/CT Could Have More Prognostic Relevance than Visual Assessment for Predicting Clinical Outcome of Extranodal Diffuse Large B Cell Lymphoma | Background/Aim: The present study retrospectively investigated the predictive accuracy of interim positron emission tomography/computed tomography (iPET/CT) based on the Deauville 5-point scale (5-PS) and a quantitative SUV-based assessment in patients with extranodal (EN) diffuse large B cell lymphoma (DLBCL). Patients and Methods: The Deauville 5-PS and the SUVmax reduction (Delta SUVmax) assessment for interpreting the response to iPET/CT were used. Results: A total of 163 patients were enrolled in this study. With a median follow- up of 52.5 months, Delta SUVmax successfully predicted the survival outcomes of patients with one extranodal (EN) involvement in terms of overall survival (OS) (p=0.012) and progression-free survival (PFS) (p<0.001). Visual assessment using the Deauville 5-PS did not predict survival outcomes in patients with one or more EN involvements in terms of OS and PFS. Conclusion: The quantitative SUV-based assessment with iPET/CT was a significant prognosticator for long-term survival outcomes, especially in patients with one EN involvement. | Baek, Dong Won; Cho, Hee Jeong; Kim, Ju-Hyung; Sohn, Sang Kyun; Song, Ga-Young; Ahn, Seo-Yeon; Jung, Sung-Hoon; Ahn, Jae Sook; Lee, Je-Jung; Kim, Hyeoung-Joon; Jeong, Shin-Young; Hong, Chae Moon; Min, Jung-Joon; Moon, Joon-Ho; Yang, Deok-Hwan | Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Dept Hematol Oncol, Sch Med, 130 Dongdeok Ro, Daegu 41944, South Korea; Chonnam Natl Univ, Hwasun Hosp, Sch Med, Dept Hematol Oncol, Hwasun, South Korea; Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Dept Nucl Med, Daegu, South Korea; Chonnam Natl Univ, Hwasun Hosp, Sch Med, Dept Nucl Med, Hwasun, South Korea | ; Lee, Jung-Hye/F-6974-2013 | 57191874272; 57216754907; 58739544500; 13310226800; 57193027251; 55945078500; 55511978300; 22984055900; 7601478211; 7410127473; 36164032500; 37050876700; 7201920094; 56568642700; 8701758000 | jhmoon@knu.ac.kr;drydh1685@hotmail.com; | IN VIVO | IN VIVO | 0258-851X | 1791-7549 | 34 | 4 | SCIE | MEDICINE, RESEARCH & EXPERIMENTAL | 2020 | 2.155 | 80.4 | 0.13 | 2025-06-25 | 3 | 4 | DLBCL; extranodal; interim PET/CT; SUV; Deauville 5-point scale | POSITRON-EMISSION-TOMOGRAPHY; NON-HODGKINS-LYMPHOMA; SUV-BASED ASSESSMENT; EARLY F-18-FDG PET; FDG-PET; INTERNATIONAL WORKSHOP; RESPONSE ASSESSMENT; ELDERLY-PATIENTS; R-CHOP; CRITERIA | Deauville 5-point scale; DLBCL; Extranodal; Interim PET/CT; SUV | Disease-Free Survival; Fluorodeoxyglucose F18; Humans; Lymphoma, Large B-Cell, Diffuse; Positron Emission Tomography Computed Tomography; Positron-Emission Tomography; Prognosis; Progression-Free Survival; Retrospective Studies; cyclophosphamide; doxorubicin; fluorodeoxyglucose; prednisolone; rituximab; vincristine; fluorodeoxyglucose f 18; adult; aged; Article; cancer prognosis; cancer survival; clinical assessment; clinical outcome; comparative study; contrast enhancement; controlled study; deauville 5 point scale; diagnostic accuracy; diffuse large B cell lymphoma; female; follow up; human; long term survival; major clinical study; male; marginal zone lymphoma; maximum standardized uptake value; overall survival; positron emission tomography-computed tomography; progression free survival; quantitative analysis; retrospective study; treatment response; diagnostic imaging; diffuse large B cell lymphoma; disease free survival; positron emission tomography; prognosis | English | 2020 | 2020 (JUL-AUG) | 10.21873/invivo.12018 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | TGFBI Expression Predicts the Survival of Patients With Oropharyngeal Squamous Cell Carcinoma | Background/Aim: This study was conducted to investigate transforming growth factor beta-induced protein (TGFBI) expression and analyze the clinical and prognostic significance of TGFBI in oropharyngeal squamous cell carcinoma (OPSCC). Patients and Methods: We evaluated TGFBI expression by immunohistochemistry in 94 patients with OPSCC. For comprehensive analysis, TGFBI expression was subdivided into tumor cell score (T), stroma score (S), and the sum of two scores (TS) calculated using H-score. Clinicopathological features and survival outcomes were compared between groups of high expression and low expression of TGFBI in each area. Results: Overall, 12 patients (12.8%) showed high T score, and 41 patients (43.6%) revealed high S score. Although T score showed no significant difference both in overall survival (OS) (p=0.080) and recurrence free survival (RFS) (p=0.272), high S score patients had significantly worse OS (p=0.003) and worse RFS (p=0.043). High TS score also showed significant association with worse OS (p=0.011) and worse RFS (p=0.021). High S score was an independent prognostic factor predicting shorter OS (HR=6.352, 95%CI=1.20640.050, p=0.029) and RFS (HR=18.843, 95%CI=1.030344.799, p=0.048) in the multivariate analysis. Conclusion: High S score of TGFBI was a significant predictor of poor prognosis in OPSCC. TGFBI could be a useful new predictive and prognostic biomarker in OPSCC. | Kim, Ha-Jeong; Ahn, Dongbin; Park, Tae-In; Jeong, Ji Yun | Kyungpook Natl Univ, Sch Med, Dept Physiol, Daegu, South Korea; Kyungpook Natl Univ, Sch Med, Dept Otolaryngol Head & Neck Surg, Daegu, South Korea; Kyungpook Natl Univ, Sch Med, Dept Pathol, Daegu, South Korea; Kyungpook Natl Univ, Chilgok Hosp, Dept Pathol, Daegu, South Korea | 57191717512; 44761055400; 7401801814; 57205472984 | jyjeong@knu.ac.kr; | IN VIVO | IN VIVO | 0258-851X | 1791-7549 | 34 | 5 | SCIE | MEDICINE, RESEARCH & EXPERIMENTAL | 2020 | 2.155 | 80.4 | 0.13 | 2025-06-25 | 4 | 3 | TGFBI; oropharyngeal squamous cell carcinoma | POOR-PROGNOSIS; MATRIX PROTEIN; GROWTH; CANCER; LUNG; ALPHA-V-BETA-3; SIGNATURE; DOMAINS | Oropharyngeal squamous cell carcinoma; TGFBI | Carcinoma, Squamous Cell; Head and Neck Neoplasms; Humans; Oropharyngeal Neoplasms; Prognosis; Squamous Cell Carcinoma of Head and Neck; Transforming Growth Factor beta; antineoplastic agent; transforming growth factor beta1; transforming growth factor beta; adult; Article; cancer localization; cancer prognosis; cancer radiotherapy; cancer staging; cancer surgery; cancer survival; chemoradiotherapy; clinical feature; controlled study; disease association; female; follow up; human; human tissue; immunohistochemistry; major clinical study; male; medical record review; middle aged; oropharynx squamous cell carcinoma; outcome assessment; overall survival; protein expression; recurrence free survival; retrospective study; risk factor; throat; genetics; head and neck tumor; oropharynx tumor; prognosis; squamous cell carcinoma | English | 2020 | 2020 (SEP-OCT) | 10.21873/invivo.12132 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Implementation of tidal parameterization in the Weather Research and Forecasting (WRF) model | In this study, the Noah land surface model with a tidal parameterization was implemented in the WRF model. Newly added inputs included gridded tidal data that indicated the presence of a tidal flat, and a time varying water layer thickness over the tidal flat. Two simulations were performed in an online mode, namely, a simulation without tidal effects (CNTL) and simulation with tidal effects (TIDE). A comparison between the simulations and observations at flux tower showed that the surface heat and momentum fluxes were well reproduced in the TIDE simulation both in the magnitude and variation. In spite of better surface flux simulations, the air temperatures were slightly better simulated in the TIDE simulation than in the CNIL simulation during nighttime only. The 10 m wind speed was slightly overestimated in the TIDE compared to the CNTL simulation. The mean differences in the field variables between the TIDE and CNTL, simulations were examined for Ibur cases during the simulation period (inundation and exposed conditions at night and inundation and exposed conditions during the day). Tidal grids were classified into two groups depending on the land use type in the CNTL simulation as either the land to tide (LT) group or the water to tide (WT) group. The magnitude of the 2 m air temperature difference between the two simulations was generally larger on LT group than on WT group. During the day, both the 2 m air temperature and the planetary boundary layer height (PBLH) decreased under inundation conditions over LT group. The impact of the tidal flat on 10 m wind and PBLH extended inland beyond the tidal flats during the daytime inundation period. | Lee, Young-Hee; Ahn, Kwang-Deuk; Lee, Yong Hee; Eom, Hyunmin | Kyungpook Natl Univ, Dept Astron & Atmospher Sci, Daegu, South Korea; Korea Meteorol Adm, Numer Data Applicat Div, Numer Modeling Ctr, Seoul, South Korea; Korea Meteorol Adm, Marine Meteorol Div, Seoul, South Korea | 56812706200; 57189992426; 47962282400; 36245980700 | young@knu.ac.kr; | TERRESTRIAL ATMOSPHERIC AND OCEANIC SCIENCES | TERR ATMOS OCEAN SCI | 1017-0839 | 2311-7680 | 31 | 1 | SCIE | GEOSCIENCES, MULTIDISCIPLINARY;METEOROLOGY & ATMOSPHERIC SCIENCES;OCEANOGRAPHY | 2020 | 1.009 | 80.5 | 0.18 | 2025-06-25 | 2 | 3 | Implementation of tidal parameterization; Surface fluxes; WRF model | SEA | Implementation of tidal parameterization; Surface fluxes; WRF model | air temperature; comparative study; computer simulation; heat flux; land surface; parameterization; surface flux; tidal flat; weather forecasting; wind velocity | English | 2020 | 2020-02 | 10.3319/tao.2019.07.03.01 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Mediation of induced systemic resistance by the plant growth-promoting rhizobacteriaBacillus pumilusS2-3-2 | Plant-rhizobacteria interaction and co-evolution developed adaptive strategies which may help the plant survive in nature. Plant rhizosphere soil isolates were analyzed to investigated the effects of rhizobacteria for promoting plant growth and suppress plant disease. Bacterial strains which isolated from plant rhizosphere soil were screened for elicitation of induced systemic resistance (ISR) on tobacco. Strain S2-3-2 results in significant reduction of disease severity on tobacco, it was identified asBacillus pumilusby multilocus sequence analysis (MLSA). Strain S2-3-2 was deeper studied for pepper plant growth promotion and biological control activity against pepper bacterial spot disease. It was found that the pepper disease severity was decreased when the roots were drenched with strain S2-3-2, and the pepper plants had a higher weight and chlorophyll content, as compared with the mock-treated plants. Transcriptional expression of pathogenesis-related (PR) protein genes in pepper was analyzed by real-time PCR, gene expressions ofCaPR1,CaPR4, andCaPR10were increased when the plants were treated with strain S2-3-2. Moreover, strain S2-3-2 was tested for the production of indole-3-acetic acid (IAA), and it was determined to emit volatiles that enhance the growth of the tobacco plants. Interesting, heat-killed S2-3-2 enhance the pepper root growth, increase the gene expressions ofCaPR4andCaPR10after pathogen challenge for 6 h, but limited to suppress the pepper bacterial spot disease as compare to the mock-treated plants. Strain S2-3-2 can be a potential biological control agent on the plant root for plant growth promoting and disease suppression. | Li, Weilan; Lee, Seung-Yeol; Cho, Young-Je; Ghim, Sa-Youl; Jung, Hee-Young | Kyungpook Natl Univ, Sch Appl Biosci, Daegu 41566, South Korea; Kyungpook Natl Univ, Inst Plant Med, Daegu 41566, South Korea; Kyungpook Natl Univ, Sch Food Sci & Biotechnol, Daegu 41566, South Korea; Kyungpook Natl Univ, Sch Life Sci, Daegu 41566, South Korea | Lee, Hye Ah/ABC-8131-2021 | 57196248967; 56106499600; 55265396300; 7003577307; 7403029383 | heeyoung@knu.ac.kr; | MOLECULAR BIOLOGY REPORTS | MOL BIOL REP | 0301-4851 | 1573-4978 | 47 | 11 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY | 2020 | 2.316 | 80.5 | 1 | 2025-06-25 | 27 | 27 | Bacterial spot disease; Biological control; Induced systemic resistance (ISR); Plant growth-promoting rhizobacteria (PGPR) | SALICYLIC-ACID; BACILLUS-PUMILUS; PV. VESICATORIA; BACTERIAL SPOT; RHIZOBACTERIA; PEPPER; STRAINS; TOLERANCE; TOBACCO; DISEASE | Bacterial spot disease; Biological control; Induced systemic resistance (ISR); Plant growth-promoting rhizobacteria (PGPR) | Bacillus pumilus; Capsicum; Disease Resistance; Gene Expression Regulation, Plant; Multilocus Sequence Typing; Plant Diseases; Plant Proteins; Plant Roots; Rhizosphere; Soil Microbiology; Tobacco; indoleacetic acid; plant protein; Article; Bacillus pumilus; bacterial plant disease; bacterial strain; bacterium identification; bacterium isolation; biological control agent; CaPR1 gene; CaPR10 gene; CaPR4 gene; controlled study; gene; gene expression; nonhuman; Pectobacterium carotovorum; plant growth; plant growth-promoting bacterium; real time polymerase chain reaction; rhizosphere; Xanthomonas axonopodis; Bacillus pumilus; disease resistance; gene expression regulation; genetics; growth, development and aging; isolation and purification; metabolism; microbiology; multilocus sequence typing; pepper; physiology; plant disease; plant root; procedures; rhizosphere; tobacco | English | 2020 | 2020-11 | 10.1007/s11033-020-05883-9 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Sunken society with Sewol Ferry: Three Korean social workers' experience | We are three Korean Social Workers who have described our experiences of the Sewol Ferry disaster using collaborative autoethnography. We were standing in the turmoil of this disaster, trying to help people in crisis, but still curious about what we can do as Social Workers to help people living in such societal crisis. As we tell our stories we have tried to understand ourselves and offer a psychosocial analysis of the social dynamics in Korean sociocultural context and the collective responses to the loss and trauma during this man-made disaster, so that we can find out what Korean social workers can do or have to do in disasters like this and further this society be better conscious through empathy. We have learned that social workers should be the ego trainer and container to help people. | Ha, Jungmi; Kim, Sunghoon; Han, Doolee | Busan Presbyterian Univ, Social Welf & Counseling, Gimhae, South Korea; Pusan Natl Univ, Social Welf, Busan, South Korea; Kyungpook Natl Univ, Social Welf, Daegu, South Korea | Kim, Youn/AAT-3380-2021 | 57201673586; 59288109800; 57201673746 | drh7219@naver.com; | JOURNAL OF SOCIAL WORK PRACTICE | J SOC WORK PRACT | 0265-0533 | 1465-3885 | 34 | 2 | SSCI | SOCIAL WORK | 2020 | 1.219 | 80.7 | 0.15 | 2025-06-25 | 3 | 3 | Autoethnography; Sewol Ferry disaster; social work | Autoethnography; Sewol Ferry disaster; social work | English | 2020 | 2020-04-02 | 10.1080/02650533.2018.1460585 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Risk of malignancy in Korean patients with primary Sjogren's syndrome | Objectives To evaluate the risks of overall and site-specific malignancies in Korean patients with primary Sjogren's syndrome (pSS). Methods Using the Korean nationwide healthcare claims database, we constructed a retrospective cohort for prevalent pSS aged over 50 years. After enrollment between January 2012 and December 2014, patients were followed until the development of any malignancy, or until December 2015. Crude incidence rates of malignancies of pSS patients were calculated, and their standardized incidence ratios (SIRs) for malignancies were calculated compared to those in knee osteoarthritis (OA) patients. Results A total of 6,359 pSS and 5,476,302 knee OA patients were included in this study. During follow-up (19,474 person-years [PYs]), 310 cases of solid malignancy (158.8/10,000 PYs) and 47 cases of hematologic malignancies (23.5/10,000 PYs) were observed in pSS patients. The risks of overall (SIR 1.30, 95% CI 1.16-1.43), solid (SIR 1.16, 95% CI 1.03-1.29), and hematologic malignancies (SIR 4.80, 95% CI 3.43-6.17) were increased in pSS patients. There was an elevated risk of site-specific malignancy in non-Hodgkin's lymphoma (NHL, SIR 6.45, 95% CI 4.05-8.83), multiple myeloma (SIR 4.88, 95% CI 2.00-7.76), and oropharynx (SIR 4.16, 95% CI 1.90-6.42). The risk of lung cancer was increased only in male pSS patients (2.50, 95% CI 1.02-3.99), while the risk of thyroid cancer was increased in female patients (1.44, 95% CI 1.04, 1.84). Conclusion In patients with pSS over age 50, the risk of solid cancers such as oropharynx, thyroid, and lung cancers is also increased in addition to NHL. | Kang, Juyeon; Kim, Hyoungyoung; Kim, Jinwook; Choi, Seongmi; Jung, Sun-Young; Jang, Eun Jin; Cho, Soo-Kyung; Sung, Yoon-Kyoung | Hanyang Univ Hosp Rheumat Dis, Dept Rheumatol, Seoul, South Korea; Kyungpook Natl Univ, Dept Stat, Deagu, South Korea; Natl Hlth Insurance Serv, Hlth Insurance Policy Res Inst, Wonju, South Korea; Chung Ang Univ, Coll Pharm, Seoul, South Korea; Andong Natl Univ, Dept Informat Stat, Andong, South Korea | 57210833095; 57202108695; 57212384364; 57211676692; 57207445502; 37861741600; 35185890800; 15754508900 | sungyk@hanyang.ac.kr; | INTERNATIONAL JOURNAL OF RHEUMATIC DISEASES | INT J RHEUM DIS | 1756-1841 | 1756-185X | 23 | 9 | SCIE | RHEUMATOLOGY | 2020 | 2.454 | 80.9 | 1.41 | 2025-06-25 | 25 | 22 | hematologic malignancy; incidence; primary Sjogren's syndrome; solid malignancy | HASHIMOTOS-THYROIDITIS; CANCER INCIDENCE; PATHOGENESIS; POPULATION; MORTALITY; LYMPHOMA; DISEASES; COHORT; NATIONWIDE; UPDATE | hematologic malignancy; incidence; primary Sjögren's syndrome; solid malignancy | Aged; Databases, Factual; Female; Humans; Incidence; Male; Middle Aged; Neoplasms; Osteoarthritis, Knee; Prevalence; Republic of Korea; Retrospective Studies; Risk Assessment; Risk Factors; Sjogren's Syndrome; Time Factors; adult; aged; Article; cancer incidence; cancer risk; cohort analysis; comparative study; disease association; female; follow up; hematologic malignancy; human; Korean (people); lip cancer; lung cancer; major clinical study; male; middle aged; multiple myeloma; nonhodgkin lymphoma; oropharynx cancer; patient risk; prevalence; priority journal; retrospective study; sex ratio; Sjoegren syndrome; solid malignant neoplasm; standardized incidence ratio; thyroid cancer; factual database; incidence; knee osteoarthritis; neoplasm; risk assessment; risk factor; Sjoegren syndrome; South Korea; time factor | English | 2020 | 2020-08 | 10.1111/1756-185x.13927 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Pasteurella multocida specific bacteriophage suppresses P. multocida-induced inflammation: identification of genes related to bacteriophage signaling by Pasteurella multocida-infected swine nasal turbinate cells | Background Although Pasteurella multocida is highly prevalent pathogen in animals and plays an important role in swine respiratory diseases, only a few studies on the use of bacteriophages specific to Pasteurella multocida disease have been reported. Objective The object of this study was to investigate the therapeutic effect of specific P. multocida bacteriophages and to identify genes related to bacteriophage signaling utilizing RNA microarrays in swine nasal turbinate cells. Methods Pas-MUP-1 phages were applied 24 h prior to P. multocida infection (1 x 10(7) cfu/ml) at several concentrations of bacterial infection. Cells were incubated to detect cytokines and 24 h to detect mucin production. And real-time quantitative PCR was performed to examine related genes expression. To determine the change of total gene expression based on P. multocida and Pas-MUP-1 treatment, we performed RNA sequencing experiments. Results We found that P. multocida-infected PT-K75 cells show increased gene expression of IL-1 beta, IL-6, and Muc1 in a dose-dependent manner. Interestingly, these genes resulted in decreased expression in P. multocida pretreated with the P. multocida-specific Pas-MUP-1 bacteriophage. RNA sequencing analysis revealed that bacteriophage administration regulated genes associated with immune and inflammatory responses, and the regulated genes were dramatically concentrated in the cytokine/chemokine-based signaling pathways. Pas-MUP-1 treatment was shown to regulate P. multocida induced gene expression in the bacteria. Conclusion These results suggest the specific bacteriophage has therapeutic potential as an alternative to antibiotic treatment to defend against P. multocida infection by altering inflammatory gene expression profiles. | Park, Ga Young; Yu, Hyun Jin; Son, Jee Soo; Park, Sang Joon; Cha, Hee-Jae; Song, Kyoung Seob | Kosin Univ, Dept Cell Biol, Coll Med, 34 Amnam Dong, Busan 49267, South Korea; iNtRON Biotechnol, Inst Life Technol, Seongnam, South Korea; Kyungpook Natl Univ, Coll Vet Med, Daegu, South Korea; Kosin Univ, Dept Parasitol & Genet, Coll Med, Busan, South Korea | Kim, Young-Il/ISS-7678-2023; Cha, Hee-Jae/AFO-8772-2022 | 57204480605; 57204478496; 35182087500; 7501825941; 7103181639; 7401740535 | kssong@kosin.ac.kr; | GENES & GENOMICS | GENES GENOM | 1976-9571 | 2092-9293 | 42 | 2 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;BIOTECHNOLOGY & APPLIED MICROBIOLOGY;GENETICS & HEREDITY | 2020 | 1.839 | 81.0 | 0.13 | 2025-06-25 | 3 | 3 | Swine nasal turbinate cells; Pasteurella multocida; IL-6; IL-1 beta; Muc1; Pas-MUP-1; microarray | BORDETELLA-BRONCHISEPTICA; PATHOGENESIS; CYTOKINE; PIGS | IL-1β, Muc1; IL-6; Pas-MUP-1, microarray; Pasteurella multocida; Swine nasal turbinate cells | Animals; Bacteriophages; Cells, Cultured; Gene Expression Profiling; Gene Ontology; Inflammation Mediators; Interleukin-1beta; Interleukin-6; Mucin-1; Pasteurella multocida; RNA, Messenger; Swine; Turbinates; complementary DNA; cytokine; interleukin 1beta; interleukin 6; microRNA; mucin 1; RNA; autacoid; interleukin 1beta; interleukin 6; messenger RNA; mucin 1; animal cell; animal experiment; animal model; Article; bacteriophage; clinical evaluation; concentration process; controlled study; cytokine production; cytokine release; gene expression; gene expression profiling; gene identification; genetic association; immune response; incubation time; microarray analysis; nonhuman; Pasteurella multocida; Pasteurella multocida infection; pig; real time polymerase chain reaction; respiratory tract inflammation; RNA sequence; signal transduction; therapy effect; turbinate; animal; cell culture; gene ontology; genetics; metabolism; microbiology; Pasteurella multocida; physiology; turbinate; virology | English | 2020 | 2020-02 | 10.1007/s13258-019-00898-4 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Prognostic value of Iroquois homeobox 1 methylation in non-small cell lung cancers | Background Non-small cell lung cancer (NSCLC) poses a great threat to human health. DNA methylation abnormalities play a central role in the development and outcome of most human malignancies, providing potential biomarkers for diagnosis and prognosis. Iroquois homeobox 1 (IRX1) can act as a tumor suppressor or promoter depending on the tumor microenvironment, and its role in lung cancer is still controversial. Objective The purpose of this study was to investigate the biological role and prognostic value of IRX1 in NSCLC. Methods We examined the methylation status of IRX1 promoter in 146 tumors from patients with NSCLC using pyrosequencing and analyzed the association between methylation status and overall patient survival. Results A total of 37 cases (25.3%) showed IRX1 methylation-positive tumors when compared with matched normal tissues. No association between IRX1 expression level and methylation status was found in lung cancer cell lines. IRX1 methylation significantly correlated with smoking status and TP53 mutation. Patients with IRX1 methylation showed significantly longer survival than patients without methylation (log-rank P = 0.011). In a multivariate analysis of prognostic factors, IRX1 methylation in tumor samples was an independent prognostic factor (adjusted hazard ratio = 0.35, 95% confidence interval 0.17-0.73, P = 0.005). Conclusion These results suggest that IRX1 promoter methylation may be a tumor-associated event and an independent predictor of survival advantage in patients with NSCLC. Further large-scale studies are needed to confirm these findings. | Lee, Ji Yun; Lee, Won Kee; Park, Jae Yong; Kim, Dong Sun | Kyungpook Natl Univ, Sch Med, Dept Anat, 2-101 Dongin Dong, Daegu 702422, South Korea; Kyungpook Natl Univ, Sch Med, BK21 Plus KNU Biomed Convergence Program, 2-101 Dongin Dong, Daegu 702422, South Korea; Kyungpook Natl Univ, Sch Med, Dept Prevent Med, Daegu 702422, South Korea; Kyungpook Natl Univ, Sch Med, Dept Internal Med, 2-101 Dongin Dong, Daegu 702422, South Korea | ; Kim, Dae/AAJ-7518-2021 | 37124481700; 22953484700; 58360293800; 57125070500 | jaeyong@knu.ac.kr;doskim@knu.ac.kr; | GENES & GENOMICS | GENES GENOM | 1976-9571 | 2092-9293 | 42 | 5 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;BIOTECHNOLOGY & APPLIED MICROBIOLOGY;GENETICS & HEREDITY | 2020 | 1.839 | 81.0 | 0.27 | 2025-06-25 | 5 | 5 | Non-small cell lung cancer; Hypermethylation; IRX1; Pyrosequencing; Prognosticator | DNA METHYLATION; PROMOTER METHYLATION; CPG ISLANDS; GENOME; GENES; IRX1; ADENOCARCINOMA; HYPERMETHYLATION; EXPRESSION; PATTERNS | Hypermethylation; IRX1; Non-small cell lung cancer; Prognosticator; Pyrosequencing | Biomarkers, Tumor; Carcinoma, Non-Small-Cell Lung; Cell Line, Tumor; DNA Methylation; Female; Homeodomain Proteins; Humans; Lung Neoplasms; Male; Middle Aged; Prognosis; Transcription Factors; cytosine; glyceraldehyde 3 phosphate dehydrogenase; iroquois homeobox 1; messenger RNA; protein p53; tumor suppressor protein; unclassified drug; homeodomain protein; IRX1 protein, human; transcription factor; tumor marker; adult; aged; Article; cancer prognosis; cancer survival; comparative study; controlled study; disease association; DNA methylation; female; gene mutation; genetic association; human; human tissue; lung cancer cell line; major clinical study; male; non small cell lung cancer; outcome assessment; overall survival; people by smoking status; promoter region; protein expression level; pyrosequencing; genetics; lung tumor; metabolism; middle aged; non small cell lung cancer; pathology; prognosis; tumor cell line | English | 2020 | 2020-05 | 10.1007/s13258-020-00925-9 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Specific bacteriophage of Bordetella bronchiseptica regulates B. bronchiseptica-induced microRNA expression profiles to decrease inflammation in swine nasal turbinate cells | Background Respiratory diseases in pigs are the main health concerns for swine producers. Similar to the diseases in human and other animals, respiratory diseases are primary related to morbidity and are the result of infection with bacteria, viruses, or both. B. bronchiseptica causes serious respiratory diseases in the swine airway track. However, the B. bronchiseptica-specific bacteriophage has diverse advantages such as decreasing antibiotic overuse and possible therapeutic potential against bacteria. Objective The objects of this study were to investigate the therapeutic effect of specific B. bronchiseptica bacteriophages and to identify genes related to bacteriophage signaling utilizing RNA microarrays in swine nasal turbinate cells. Methods Bor-BRP-1 phages were applied 24 h prior to B.bronchiseptica infection (1 x 10(7) cfu/ml) at several concentrations of bacterial infection. Cells were incubated to detect cytokines and 24 h to detect mucin production. And real-time quantitative PCR was performed to examine related genes expression. To determine the change of total gene expression based on B.bronchiseptica and Bor-BRP-1 treatment, we performed RNA sequencing experiments. Results The results showed that B. bronchiseptica induced increased expression of several inflammatory genes such as IL-1 beta, IL-6, and Muc1 in a dose-dependent manner. However, Bor-BRP-1 induced reduction of gene expression compared to the B. bronchiseptica induction group. In addition, microarrays detected Bor-BRP-1-altered inflammatory gene expression against B. bronchiseptica, reducing B. bronchiseptica-induced airway inflammation in swine epithelial cells. Conclusion These results suggest that the specific bacteriophage has a therapeutic potential to defend against B. bronchiseptica infection by altering inflammatory gene expression profiles. | Park, Ga Young; Yu, Hyun Jin; Son, Jee Soo; Park, Sang Joon; Cha, Hee-Jae; Song, Kyoung Seob | Kosin Univ, Dept Cell Biol, Coll Med, 34 Amnam Dong, Busan 49267, South Korea; INtRON Biotechnol, Inst Life Technol, Seongnam, South Korea; Kyungpook Natl Univ, Coll Vet Med, Daegu, South Korea; Kosin Univ, Dept Parasitol & Genet, Coll Med, Busan, South Korea | Kim, Young-Il/ISS-7678-2023; Cha, Hee-Jae/AFO-8772-2022 | 57204480605; 57204478496; 35182087500; 7501825941; 7103181639; 7401740535 | kssong@kosin.ac.kr; | GENES & GENOMICS | GENES GENOM | 1976-9571 | 2092-9293 | 42 | 4 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;BIOTECHNOLOGY & APPLIED MICROBIOLOGY;GENETICS & HEREDITY | 2020 | 1.839 | 81.0 | 0.07 | 2025-06-25 | 2 | 2 | Swine nasal turbinate cells; Bordetella bronchiseptica; IL-6; IL-1 beta; Muc1; Bor-BRP-1; Microarray | RESPIRATORY-TRACT; BACTERIAL PATHOGENS; STREPTOCOCCUS-SUIS; DISEASE; TRANSMISSION; THERAPY; PHAGE; PIGS | Bor-BRP-1; Bordetella bronchiseptica; IL-1 β; IL-6; Microarray; Muc1; Swine nasal turbinate cells | Animals; Bacteriophages; Bordetella bronchiseptica; Bordetella Infections; Cells, Cultured; Interleukins; MicroRNAs; Mucin-1; Swine; Swine Diseases; Transcriptome; Turbinates; interleukin 1beta; interleukin 6; microRNA; mucin 1; interleukin derivative; microRNA; mucin 1; transcriptome; AMPK signaling; animal cell; Article; Bordetella bronchiseptica; Bordetella bronchiseptica infection; cell viability assay; controlled study; cytokine production; cytokine release; gene expression profiling; hierarchical clustering; inflammation; microarray analysis; nonhuman; phage therapy; Pi3K/Akt signaling; pig; RNA sequencing; turbinate; animal; bacteriophage; Bordetella bronchiseptica; bordetellosis; cell culture; cytology; genetics; metabolism; microbiology; pathogenicity; swine disease; turbinate; veterinary medicine; virology | English | 2020 | 2020-04 | 10.1007/s13258-019-00906-7 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | The Prognostic Significance of TERT Locus Polymorphism (rs36115365) in Surgically Resected Non-Small Cell Lung Cancer | Objective. Telomere length is an important factor for the development of non-small cell lung cancer (NSCLC), and current articles focused on telomere associated genes. We studied the clinicopathological and prognostic implications of rs36115365 polymorphism of the TERT-CLPTM1L locus in NSCLC. The association between rs36115365 and telomere length was investigated in 176 NSCLCs. Methods. DNA was extracted from NSCLC tissues and polymorphism and telomere length were analyzed. Results. The rs36115365 polymorphism showed the following frequencies according to the genotype: G/G in 81.8% of the patients, G/C in 14.2%, and C/C in 4.0%. Average telomere length in the tumor tissues were 3.06-fold longer than telomeres in paired non-tumor tissues (SD=1.87), and telomere length was not significantly different according to rs36115365 (p=0.134). The rs36115365 polymorphism did not have any relationships with clinicopathological characteristics. A poor overall survival result was found in NSCLC with C allele carriers than that with G/G allele (p=0.034). However, disease free survival rate was not different statistically (p=0.938). Conclusions. These findings suggest that rs36115365 may contribute to the progression of NSCLC. | Lee, Deok Heon; Jung, Soo-Jung; Park, Won-Jin; Heo, Yu-Ran; Lee, Jae-Ho | Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Dept Thorac & Cardiovasc Surg, Sch Med, Daegu, South Korea; Keimyung Univ, Sch Med, Dongsan Med Ctr, Dept Anat, Daegu, South Korea | Lee, Jae-Ho/I-1935-2019 | 39561353900; 57193347340; 55448891100; 57194457061; 55224798300 | anato82@dsmc.or.kr; | ANNALS OF CLINICAL AND LABORATORY SCIENCE | ANN CLIN LAB SCI | 0091-7370 | 1550-8080 | 50 | 5 | SCIE | MEDICAL LABORATORY TECHNOLOGY | 2020 | 1.256 | 81.0 | 0 | 2025-06-25 | 0 | 0 | rs36115365 polymorphism; non-small cell lung cancer; TERT-CLPTM1L locus; telomere length | TELOMERASE REVERSE-TRANSCRIPTASE; CHROMOSOME 5P15.33; ASSOCIATION; LENGTH | non-small cell lung cancer; rs36115365 polymorphism; telomere length; TERT-CLPTM1L locus | Aged; Aged, 80 and over; Alleles; Carcinoma, Non-Small-Cell Lung; Female; Gene Frequency; Genetic Predisposition to Disease; Genotype; Humans; Lung Neoplasms; Male; Middle Aged; Polymorphism, Single Nucleotide; Prognosis; Telomerase; Telomere; Telomere Homeostasis; telomerase; TERT protein, human; aged; allele; female; gene frequency; genetic predisposition; genetics; genotype; human; lung tumor; male; metabolism; middle aged; non small cell lung cancer; pathology; prognosis; single nucleotide polymorphism; telomere; telomere homeostasis; very elderly | English | 2020 | 2020 (SEP-OCT) | 바로가기 | 바로가기 | 바로가기 |
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