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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | ○ | Article | Enhancement of Drought-Stress Tolerance of Brassica oleracea var. italica L. by Newly Isolated Variovorax sp. YNA59 | Drought is a major abiotic factor and has drastically reduced crop yield globally, thus damaging the agricultural industry. Drought stress decreases crop productivity by negatively affecting crop morphological, physiological, and biochemical factors. The use of drought tolerant bacteria improves agricultural productivity by counteracting the negative effects of drought stress on crops. In this study, we isolated bacteria from the rhizosphere of broccoli field located in Daehaw-myeon, Republic of Korea. Sixty bacterial isolates were screened for their growth-promoting capacity, in vitro abscisic acid (ABA), and sugar production activities. Among these, bacterial isolates YNA59 was selected based on their plant growth-promoting bacteria traits, ABA, and sugar production activities. Isolate YNA59 highly tolerated oxidative stress, including hydrogen peroxide (H2O2) and produces superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) activities in the culture broth. YNA59 treatment on broccoli significantly enhanced plant growth attributes, chlorophyll content, and moisture content under drought stress conditions. Under drought stress, the endogenous levels of ABA, jasmonic acid (JA), and salicylic acid (SA) increased; however, inoculation of YNA59 markedly reduced ABA (877 +/- 22 ng/g) and JA (169.36 +/- 20.74 ng/ g) content, while it enhanced SA levels (176.55 +/- 9.58 ng/g). Antioxidant analysis showed that the bacterial isolate YNA59 inoculated into broccoli plants contained significantly higher levels of SOD, CAT, and APX, with a decrease in GPX levels. The bacterial isolate YNA59 was therefore identified as Variovorax sp. YNA59. Our current findings suggest that newly isolated drought tolerant rhizospheric Variovorax sp. YNA59 is a useful stress-evading rhizobacterium that improved droughtstress tolerance of broccoli and could be used as a bio-fertilizer under drought conditions. | Kim, Yu-Na; Khan, Muhammad Aaqil; Kang, Sang-Mo; Hamayun, Muhammad; Lee, In-Jung | Kyungpook Natl Univ, Sch Appl Biosci, Daegu 41566, South Korea; Kyungpook Natl Univ, Inst Agr Sci & Technol, Daegu 41566, South Korea; Abdul Wali Khan Univ, Dept Bot, Mardan, Pakistan | Lee, In-Jung/GLS-0432-2022; Kang, Sang-Mo/MBG-7823-2025; Khan, Muhammad/ABB-9797-2021; Hamayun, Muhammad/B-1694-2010 | 57217182101; 57188585606; 56189696900; 59297330100; 16425830900 | ijlee@knu.ac.kr; | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | J MICROBIOL BIOTECHN | 1017-7825 | 1738-8872 | 30 | 10 | SCIE | BIOTECHNOLOGY & APPLIED MICROBIOLOGY;MICROBIOLOGY | 2020 | 2.351 | 72.6 | 2.28 | 2025-06-25 | 39 | 45 | Variovorax sp. YNA59; drought stress; phytohormones; antioxidant activity; broccoli | GROWTH-PROMOTING BACTERIA; SALICYLIC-ACID; PLANT-GROWTH; WATER-STRESS; SALT STRESS; RESISTANCE; INDUCTION; BROCCOLI; MAIZE; THERMOTOLERANCE | Antioxidant activity; Broccoli; Drought stress; Phytohormones; Variovorax sp. YNA59 | Abscisic Acid; Antioxidants; Brassica; Catalase; Chlorophyll; Comamonadaceae; Cyclopentanes; Droughts; Hydrogen Peroxide; Oxidative Stress; Oxylipins; Plant Development; Plant Growth Regulators; Reactive Oxygen Species; Rhizosphere; Stress, Physiological; Superoxide Dismutase; abscisic acid; ascorbate peroxidase; catalase; chlorophyll; hydrogen peroxide; jasmonic acid; salicylic acid; sugar; superoxide dismutase; abscisic acid; antioxidant; catalase; chlorophyll; cyclopentane derivative; jasmonic acid; oxylipin; phytohormone; reactive oxygen metabolite; superoxide dismutase; Article; bacterium isolate; broccoli; controlled study; drought stress; enzyme activity; in vitro study; moisture; nonhuman; oxidative stress; plant growth; rhizosphere; Variovorax; Brassica; classification; Comamonadaceae; drought; isolation and purification; metabolism; microbiology; physiological stress; physiology; plant development | English | 2020 | 2020-10 | 10.4014/jmb.2006.06010 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Folate-Gold-Bilirubin Nanoconjugate Induces Apoptotic Death in Multidrug-Resistant Oral Carcinoma Cells | Background Gold nanoparticles (GNPs) are receiving increasing attention as drug delivery carriers due to their high surface-to-volume ratio, hydrophilicity, and functionality. Drug delivery by nanocarriers has the potential to bypass P-glycoprotein (P-gp)-mediated multidrug resistance (MDR) by altering the drug internalization mechanism and/or intracellular release pattern, inhibiting the activity of ABC-transporter efflux pumps, or downregulating the expression of genes responsible for the activity of efflux pumps. Objective We developed a folate-gold-bilirubin (FGB) nanoconjugate to reverse MDR in P-expressing KB-Ch (R)-8-5 cells. Methods The P-gp overexpressing KB-Ch (R)-8-5 cells were incubated with the FGB nanoconjugate, bilirubin, or GNPs. Various cellular endpoints, such as cytotoxicity, ROS generation, DNA damage, and apoptosis, were analyzed using analytical methods. Further, a KB-Ch (R)-8-5 cell-bearing tumor xenograft was developed and the anticancer potential of the prepared FGB nanoparticles was compared to that of bilirubin or GNPs in this preclinical model. Results The FGB nanoconjugate was found to be a stronger inhibitor of the viability of multidrug-resistant KB-Ch (R)-8-5 cells than bilirubin and GNPs treatment alone. The nanoconjugate induced reactive oxygen species (ROS) formation, DNA strand breaks, and apoptotic morphological changes in the P-gp-overexpressing drug-resistant cells to a greater degree than bilirubin treatment alone. Also, the FGB nanoparticles led to stronger suppression of tumor development in the KB-Ch (R)-8-5 xenograft mouse model than achieved with bilirubin treatment alone. Thus, the present results suggest that the FGB nanoconjugate suppresses tumor growth in drug-resistant tumor cells by inducing apoptotic cell death. Conclusion FGB nanoparticles significantly inhibit tumor growth, probably through the folate receptor, which is highly expressed in KB cells. Hence, folate-gold-bilirubin nanoparticles could be a promising agent for inducing apoptosis in P-gp-overexpressing drug-resistant cancer cells. Graphic | Rathinaraj, Pierson; Muthusamy, Ganesan; Prasad, Nagarajan Rajendra; Gunaseelan, Srithar; Kim, Boeun; Zhu, Suhang | Waikato Inst Technol, Ctr Int Res & Internships, Hamilton, New Zealand; Annamalai Univ, Dept Biochem & Biotechnol, Annamalainagar, Tamil Nadu, India; Kyungpook Natl Univ, Dept Chem Engn, Daegu, South Korea; Jinhua Polytech, Dept Mech Engn, Jinhua, Zhejiang, Peoples R China | N, Rajendra Prasad/X-1743-2019; MUTHUSAMY, GANESAN/AFH-1605-2022 | 56024868100; 54952120800; 8063432700; 56940631700; 57212508306; 57212509864 | pierson.rathinaraj@wintec.ac.nz; | EUROPEAN JOURNAL OF DRUG METABOLISM AND PHARMACOKINETICS | EUR J DRUG METAB PH | 0378-7966 | 2107-0180 | 45 | 2 | SCIE | PHARMACOLOGY & PHARMACY | 2020 | 2.448 | 72.6 | 1.78 | 2025-06-25 | 24 | 25 | DRUG-DELIVERY; CANCER; NANOPARTICLE; MODULATORS; ENCAPSULATION; EXPRESSION; DESIGN | Animals; Antineoplastic Agents; Apoptosis; ATP Binding Cassette Transporter, Subfamily B; Bilirubin; Drug Delivery Systems; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Folic Acid; Gold; Humans; KB Cells; Metal Nanoparticles; Mice; Mice, Nude; Mouth Neoplasms; Nanoconjugates; Xenograft Model Antitumor Assays; ABC transporter subfamily B; bilirubin; folic acid; gold nanoparticle; nanoconjugate; reactive oxygen metabolite; ABCB1 protein, human; antineoplastic agent; bilirubin; folic acid; gold; metal nanoparticle; nanoconjugate; animal experiment; animal model; apoptosis; Article; cancer inhibition; carcinoma cell line; cell viability; controlled study; cytotoxicity; DNA damage; DNA strand breakage; human; human cell; human tissue; in vivo study; KB-ChR-8-5 cell line; material coating; mouse; mouth carcinoma; nonhuman; protein expression; animal; apoptosis; chemistry; comparative study; drug delivery system; drug effect; drug resistance; drug screening; genetics; KB cell line; mouth tumor; multidrug resistance; nude mouse | English | 2020 | 2020-04 | 10.1007/s13318-019-00600-9 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Inoculation with Indole-3-Acetic Acid-Producing Rhizospheric Rhodobacter sphaeroides KE149 Augments Growth of Adzuki Bean Plants Under Water Stress | The use of plant growth-promoting rhizobacteria is economically viable and environmentally safe for mitigating various plant stresses. Abiotic stresses such as flood and drought are a serious threat to modern agriculture. In the present study, the indole-3-acetic acid-producing rhizobacterium R. sphaeroides KE149 was selected, and its effects on the growth of adzuki bean plants under flood stress (FS) and drought stress (DS) were investigated. IAA quantification of bacterial pure culture revealed that KE149 produced a significant amount of IAA. Moreover, KE149 inoculation notably decreased stress-responsive endogenous abscisic acid and jasmonic acid and increased salicylic acid in plants under DS and FS. KE149 inoculation also increased proline under DS and methionine under FS. In addition, KE149 inoculation significantly increased the levels of calcium (Ca), magnesium (Mg), and potassium (K) while lowering the sodium (Na) content in the plant shoot under stress. KE149-treated plants had markedly greater root length, shoot length, stem diameter, biomass, and higher chlorophyll content under both normal and stressed conditions. These results suggest that KE149 could be an efficient biofertilizer for mitigating water stress. | Kang, Sang-Mo; Adhikari, Arjun; Lee, Ko-Eun; Khan, Muhammad Aaqil; Khan, Abdul Latif; Shahzad, Raheem; Dhungana, Sanjeev Kumar; Lee, In-Jung | Kyungpook Natl Univ, Sch Appl Biosci, Daegu 41566, South Korea; Univ Nizwa, Nat & Med Sci Res Ctr, Nizwa 616, Oman; Imam Abdulrahman Bin Faisal Univ, Dept Biol, Coll Sci, POB 1982, Damam 31441, Saudi Arabia; Imam Abdulrahman Bin Faisal Univ, Basic & Appl Sci Res Ctr, POB 1982, Damam 31441, Saudi Arabia; Natl Inst Crop Sci Rural Dev Adm, Dept Southern Area Crop Sci, Miryang 50424, South Korea | Khan, Abdul/B-1005-2010; Khan, Muhammad/ABB-9797-2021; Adhikari, Arjun/AAV-6297-2021; Kang, Sang-Mo/MBG-7823-2025; Dhungana, Sanjeev/O-4097-2017; Dhungana, Sanjeev Kumar/O-4097-2017; Lee, In-Jung/GLS-0432-2022; Adhikari, Arjun/JCO-3306-2023; Shahzad, Raheem/AAG-8370-2019 | 56189696900; 57195601415; 56191302700; 57188585606; 26639372800; 56454250900; 56269940800; 16425830900 | ijlee@knu.ac.kr; | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | J MICROBIOL BIOTECHN | 1017-7825 | 1738-8872 | 30 | 5 | SCIE | BIOTECHNOLOGY & APPLIED MICROBIOLOGY;MICROBIOLOGY | 2020 | 2.351 | 72.6 | 1.22 | 2025-06-25 | 21 | 21 | Abiotic stress; drought; flood; phytohormones; Rhodobacter sphaeroides KE149 | SALICYLIC-ACID; AMINO-ACIDS; SALINITY; ANTIOXIDANTS; TOLERANCE; DROUGHT; AMELIORATION; POLYPHENOLS; SEEDLINGS; YIELD | Abiotic stress; Drought; Flood; Phytohormones; Rhodobacter sphaeroides KE149 | Droughts; Floods; Indoleacetic Acids; Plant Growth Regulators; Rhizosphere; Rhodobacter sphaeroides; Stress, Physiological; Vigna; 1,1 diphenyl 2 picrylhydrazyl; abscisic acid; calcium; chlorophyll; indoleacetic acid; jasmonic acid; magnesium; methionine; polyphenol; potassium; proline; salicylic acid; sodium; indoleacetic acid derivative; phytohormone; adzuki bean; amino acid analysis; Article; bacterial strain; bacterium culture; biomass; drought stress; extraction; flooding; inoculation; nonhuman; plant growth; plant stem; Rhodobacter sphaeroides; Rhodobacter sphaeroides KE149; root length; shoot; water stress; drought; drug effect; metabolism; microbiology; physiological stress; physiology; rhizosphere; Rhodobacter sphaeroides; Vigna | English | 2020 | 2020-05 | 10.4014/jmb.1911.11063 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Optimization and Pretreatment for Hot Water Extraction of Korean Deer (Cervus canadensis Erxleben) Velvet Antlers | Velvet antler (VA) is a historically traditional medicinal supplement and is well known in Asian countries for its pharmaceutical and health benefits. The objectives for this study were to optimize the hot water extraction (HWE) of VA for the Korean VA industry, and to determine the most effective pretreatment method among microwave (MW), ultrasonication (US), and enzymatic (EZ) techniques. Using response surface methodology, optimum extraction temperatures and times were determined by central composite design configuration based on extraction yield and sialic acid content. Various quality parameters of VA extract including yield, soluble solid, protein, and sialic acid contents were also compared with the conjunction of HWE and pretreatment. The yield and sialic acid content of VA extract were determined to be 40% and 0.73 mg/g, respectively, under an optimum temperature of 100 degrees C at 24 h of extraction time. The yields from VA extracts pretreated with MW, US, and EZ were 17.42%, 19.73%, and 29.15%, respectively. Among the tested commercial enzymes, pepsin was the most effective proteolytic enzyme and led to the highest yield (47.65%), soluble solids (4.03 degrees brix), protein (1.12 mg/ml), and sialic acid (3.04 mg/ml) contents from VA extract. | Jang, Dong Wook; Ameer, Kashif; Oh, Jun-Hyun; Park, Mi-Kyung | Kyungpook Natl Univ, Sch Food Sci & Biotechnol, Daegu 41566, South Korea; Chonnam Natl Univ, Dept Food Sci & Technol, Grad Sch, Gwangju 61186, South Korea; Chonnam Natl Univ, Grad Sch, BK 21 Plus Program, Gwangju 61186, South Korea; PMAS Arid Agr Univ, Inst Food & Nutr Sci, Rawalpindi 46300, Pakistan; Sangmyung Univ, Dept Plant & Food Sci, Choenan 31066, South Korea; Kyungpook Natl Univ, Food & Bioind Res Inst, Daegu 41566, South Korea | Park, Mi-Kyung/J-9643-2017; Ameer, Kashif/H-5449-2019; Oh, Jun-Hyun/J-7704-2017 | 57210994911; 57190066397; 36451213600; 7404491155 | parkmik@knu.ac.kr; | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | J MICROBIOL BIOTECHN | 1017-7825 | 1738-8872 | 30 | 8 | SCIE | BIOTECHNOLOGY & APPLIED MICROBIOLOGY;MICROBIOLOGY | 2020 | 2.351 | 72.6 | 0.41 | 2025-06-25 | 8 | 11 | Velvet antler; response surface methodology; Cervus canadensis Erxleben; sialic acid | SERINE-PROTEASE MECHANISM; CATALYTIC MECHANISM; SIALIC-ACID; ANTIOXIDANT; PURIFICATION; PEPTIDES; ELAPHUS; PEPSIN; FAMILY; PAPAIN | Cervus canadensis Erxleben; Response surface methodology; Sialic acid; Velvet antler | Animals; Antlers; Complex Mixtures; Deer; Hot Temperature; Medicine, Traditional; N-Acetylneuraminic Acid; Republic of Korea; Temperature; Water; hot water; pepsin A; proteinase; sialic acid; n acetylneuraminic acid; water; Article; catalysis; enzyme assisted extraction; extraction; hydrolysis; microwave assisted extraction; nonhuman; pH; surface property; temperature; wapiti; animal; antler; chemistry; deer; drug mixture; heat; South Korea; traditional medicine | English | 2020 | 2020-08 | 10.4014/jmb.2004.04009 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Prevalence and Characterization of Plasmid-Mediated Quinolone Resistance Determinants qnr and aac(6′)-Ib-cr in Ciprofloxacin-Resistant Escherichia coli Isolates from Commercial Layer in Korea | The prevalence and characterization of plasmid-mediated quinolone resistance (PMQR) determinants in ciprofloxacin-resistant Escherichia coli isolated from a Korean commercial layer farm were studied. A total of 45 ciprofloxacin- resistant E. coli isolates were recovered and all isolates were multidrug-resistant. Eight isolates have the PMQR genes aac(6')-Ib-cr, qnrS1, and qnrB4, and seven isolates exhibited double amino acid exchange at both gyrA and parC, and have high fluoroquinolone minimum inhibitory concentrations. Five transconjugants demonstrated transferability of PMQR and ss-lactamase genes and similar antimicrobial resistance. Because PMQR genes in isolates from commercial layer chickens could enter the food supply and directly affect humans, control of ciprofloxacin resistance is needed. | Seo, Kwang Won; Lee, Young Ju | Kyungpook Natl Univ, Coll Vet Med, Daegu 41566, South Korea; Kyungpook Natl Univ, Zoonoses Res Inst, Daegu 41566, South Korea; Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA | 57203233812; 56612134500 | youngju@knu.ac.kr; | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | J MICROBIOL BIOTECHN | 1017-7825 | 1738-8872 | 30 | 8 | SCIE | BIOTECHNOLOGY & APPLIED MICROBIOLOGY;MICROBIOLOGY | 2020 | 2.351 | 72.6 | 0.41 | 2025-06-25 | 7 | 6 | Antimicrobial resistance; commercial layer chicken; Escherichia coli; fluoroquinolones; plasmid-mediated quinolone resistance | SPECTRUM-BETA-LACTAMASE; SALMONELLA; MECHANISMS; GENES | Antimicrobial resistance; Commercial layer chicken; Escherichia coli; Fluoroquinolones; Plasmid-mediated quinolone resistance | Animals; Anti-Bacterial Agents; beta-Lactamases; Chickens; Ciprofloxacin; DNA Gyrase; Drug Resistance, Multiple, Bacterial; Escherichia coli; Escherichia coli Proteins; Fluoroquinolones; Food Supply; Humans; Intracellular Signaling Peptides and Proteins; Microbial Sensitivity Tests; Plasmids; Quinolones; Republic of Korea; ciprofloxacin; quinoline derived antiinfective agent; antiinfective agent; beta lactamase; ciprofloxacin; DNA topoisomerase (ATP hydrolysing); Escherichia coli protein; Qnr protein, E coli; qnrS1 protein, E coli; quinolone derivative; signal peptide; aac(6') Ib cr gene; antibiotic resistance; Article; bacterial gene; bacterium isolate; ciprofloxacin resistant Escherichia coli; controlled study; gene function; gyrA gene; Korea; minimum inhibitory concentration; nonhuman; parC gene; plasmid; predictive value; prevalence; qnr gene; qnrB4 gene; qnrS1 gene; animal; catering service; chicken; drug effect; Escherichia coli; genetics; human; isolation and purification; microbial sensitivity test; multidrug resistance; plasmid; South Korea | English | 2020 | 2020-08 | 10.4014/jmb.2003.03058 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Review | Recent Insights into Aeromonas salmonicida and Its Bacteriophages in Aquaculture: A Comprehensive Review | The emergence and spread of antimicrobial resistance in pathogenic bacteria of fish and shellfish have caused serious concerns in the aquaculture industry, owing to the potential health risks to humans and animals. Among these bacteria, Aeromonas salmonicida, which is one of the most important primary pathogens in salmonids, is responsible for significant economic losses in the global aquaculture industry, especially in salmonid farming because of its severe infectivity and acquisition of antimicrobial resistance. Therefore, interest in the use of alternative approaches to prevent and control A. salmonicida infections has increased in recent years, and several applications of bacteriophages (phages) have provided promising results. For several decades, A. salmonicida and phages infecting this fish pathogen have been thoroughly investigated in various research areas including aquaculture. The general overview of phage usage to control bacterial diseases in aquaculture, including the general advantages of this strategy, has been clearly described in previous reviews. Therefore, this review specifically focuses on providing insights into the phages infecting A. salmonicida, from basic research to biotechnological application in aquaculture, as well as recent advances in the study of A. salmonicida. | Park, Seon Young; Han, Jee Eun; Kwon, Hyemin; Park, Se Chang; Kim, Ji Hyung | Korea Res Inst Biosci & Biotechnol, Infect Dis Res Ctr, Daejeon 34141, South Korea; Chungnam Natl Univ, Coll Agr & Life Sci, Div Anim & Dairy Sci, Daejeon 34134, South Korea; Kyungpook Natl Univ, Coll Vet Med, Lab Aquat Biomed, Daegu 41566, South Korea; Seoul Natl Univ, Coll Vet Med, Lab Aquat Biomed, Seoul 08826, South Korea; Seoul Natl Univ, Res Inst Vet Sci, Seoul 08826, South Korea; Korea Univ Sci & Technol UST, KRIBB Sch Biosci, Dept Biomol Sci, Daejeon 34141, South Korea | Park, Chang/AAT-3439-2021; Kim, Ji Hyung/B-7321-2011 | 57208480038; 57214671240; 57219719164; 25925284000; 57225000204 | parksec@snu.ac.kr;kzh81@kribb.re.kr; | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | J MICROBIOL BIOTECHN | 1017-7825 | 1738-8872 | 30 | 10 | SCIE | BIOTECHNOLOGY & APPLIED MICROBIOLOGY;MICROBIOLOGY | 2020 | 2.351 | 72.6 | 0.74 | 2025-06-25 | 48 | 53 | Aeromonas salmonicida; antimicrobial resistance; salmonid culture; bacteriophage | SALMO-SALAR L.; TROUT ONCORHYNCHUS-MYKISS; ATLANTIC SALMON; SUBSP SALMONICIDA; MOLECULAR CHARACTERIZATION; ANTIBIOTIC-RESISTANCE; INTERNATIONAL COMMITTEE; TETRACYCLINE-RESISTANT; T4-LIKE BACTERIOPHAGE; BIOLOGICAL-CONTROL | Aeromonas salmonicida; Antimicrobial resistance; Bacteriophage; Salmonid culture | Aeromonas salmonicida; Animals; Aquaculture; Bacterial Infections; Bacteriophages; Fish Diseases; Fishes; beta lactam antibiotic; florfenicol; folic acid; quinolone derivative; tetracycline derivative; Aeromonas salmonicida; antibiotic resistance; aquaculture; bacterial strain; bacteriophage; bacterium isolate; biotechnology; clustered regularly interspaced short palindromic repeat; disease control; disease transmission; DNA damage; electron microscopy; Food and Drug Administration; gene locus; gene sequence; genomics; microbial community; nonhuman; Oncorhynchus mykiss; plasmid; prevalence; Review; Salmo salar; Salvelinus fontinalis; shellfish; ultraviolet irradiation; vaccination; Aeromonas salmonicida; animal; bacterial infection; bacteriophage; fish; fish disease; isolation and purification; metabolism; microbiology; virology | English | 2020 | 2020-10 | 10.4014/jmb.2005.05040 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Smilax guianensis Vitman Extract Prevents LPS-Induced Inflammation by Inhibiting the NF-κB Pathway in RAW 264.7 Cells | Nutraceutical treatments can reduce inflammation and prevent the development of inflammatory diseases. In this study, the anti-inflammatory effects of Smilax guianensis Vitman extract (SGE) were examined. SGE suppressed lipopolysaccharide (LPS)-mediated nitrite production in RAW 264.7 cells. SGE also prevented the LPS-induced expression of inducible nitric oxide synthase (iNOS) but not cyclooxygenase (COX)-2. Western blot analysis showed that SGE attenuated LPS-induced phosphorylation of I kappa B kinase (IKK), inhibitor of kappa B (I kappa B), and p65. Additionally, SGE inhibited LPS-induced I kappa B degradation in RAW 264.7 cells. Western blot analysis of the cytosolic and nuclear fractions, as well as immunofluorescence assay results, revealed that SGE suppressed LPS-induced p65 nuclear translocation in RAW 264.7 cells. Moreover, SGE reduced LPS-induced interleukin (IL)-10, IL-6, and tumor necrosis factor-alpha (TNF-alpha) mRNA expression and IL-10 and IL-6 protein expression in RAW 264.7 cells. Collectively, these results indicate that SGE suppresses the NF-alpha B signaling pathway and thereby inhibits the production of NO, IL-10, and IL-6. | Kim, Ju Gyeong; Kim, Min Jeong; Lee, Ji Su; Sydara, Kongmany; Lee, Sangwoo; Byun, Sanguine; Jung, Sung Keun | Kyungpook Natl Univ, Sch Food Sci & Biotechnol, Daegu 41566, South Korea; Incheon Natl Univ, Div Bioengn, Incheon 22012, South Korea; Minist Hlth, Inst Tradit Med, Viangchan 116, Laos; Korea Res Inst Biosci & Biotechnol, Int Biol Mat Res Ctr, Daejeon 34141, South Korea; Yonsei Univ, Dept Biotechnol, Seoul 03722, South Korea; Kyungpook Natl Univ, Inst Agr Sci & Technol, Daegu 41566, South Korea | Jung, SUNG KEUN/AGR-2623-2022; Byun, Sanguine/AAR-5451-2021; Lee, Sang/P-4462-2019 | 57216640718; 57215818497; 57201996340; 6505874529; 7601398671; 24490630000; 35310491400 | skjung04@knu.ac.kr; | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | J MICROBIOL BIOTECHN | 1017-7825 | 1738-8872 | 30 | 6 | SCIE | BIOTECHNOLOGY & APPLIED MICROBIOLOGY;MICROBIOLOGY | 2020 | 2.351 | 72.6 | 0.73 | 2025-06-25 | 13 | 16 | Smilax guianensis Vitman extract; nutraceuticals; nitric oxide; nitric oxide synthase; inflammation; NF-kappa B | OXIDE SYNTHASE INOS; SIGNALING PATHWAY; COX-2; EXPRESSION; MACROPHAGES; ACTIVATION; AP-1; NO | Inflammation; NF-κB; Nitric oxide; Nitric oxide synthase; Nutraceuticals; Smilax guianensis Vitman extract | Animals; Cell Survival; Dietary Supplements; Inflammation; Mice; NF-kappa B; Nitric Oxide; Nitric Oxide Synthase; Plant Extracts; RAW 264.7 Cells; Signal Transduction; Smilax; antiinflammatory agent; cyclooxygenase 2; I kappa B kinase; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; interleukin 1beta; interleukin 6; lipopolysaccharide; messenger RNA; nitric oxide; nitrite; plant extract; Smilax guianensis extract; transcription factor RelA; tumor necrosis factor; unclassified drug; antiinflammatory agent; I kappa B kinase inhibitor; plant extract; Smilax guianensis extract; synaptotagmin I; vitman extract; immunoglobulin enhancer binding protein; nitric oxide; nitric oxide synthase; plant extract; animal cell; antiinflammatory activity; Article; cell nucleus; controlled study; cytosol; enzyme linked immunosorbent assay; enzyme phosphorylation; immunofluorescence; lipopolysaccharide-induced inflammation; macrophage culture; mouse; nonhuman; protein expression; RAW 264.7 cell line; real time polymerase chain reaction; signal transduction; Western blotting; cellular distribution; cytokine production; cytosolic fraction; enzyme degradation; gene expression; immunofluorescence test; sarsaparilla; Smilax guianensis; animal; cell survival; chemistry; dietary supplement; drug effect; inflammation; metabolism; RAW 264.7 cell line; signal transduction | English | 2020 | 2020-06 | 10.4014/jmb.1911.11042 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Trehalose Protects the Probiotic Yeast Saccharomyces boulardii against Oxidative Stress-Induced Cell Death | Saccharomyces boulardii is the only probiotic yeast with US Food and Drug Administration approval. It is routinely used to prevent or treat acute diarrhea and other gastrointestinal disorders, including the antibiotic-associated diarrhea caused by Clostridium difficile infections. The formation of reactive oxygen species (ROS), specifically H2O2 during normal aerobic metabolism, contributes to programmed cell death and represents a risk to the viability of the probiotic microbe. Moreover, a loss of viability reduces the efficacy of the probiotic treatment. Therefore, inhibiting the accumulation of ROS in the oxidant environment could improve the viability of the probiotic yeast and lead to more efficacious treatment. Here, we provide evidence that supplementation with a non-reducing disaccharide, namely trehalose, enhanced the viability of S. boulardii exposed to an oxidative environment by preventing metacaspase YCA1-mediated programmed cell death through inhibition of intracellular ROS production. Our results suggest that supplementation with S. boulardii together with trehalose could increase the viability of the organism, and thus improve its effectiveness as a probiotic and as a treatment for acute diarrhea and other gastrointestinal disorders. | Moon, Ji Eun; Heo, Wan; Lee, Sang Hoon; Lee, Suk Hee; Lee, Hong Gu; Lee, Jin Hyup; Kim, Young Jun | Korea Univ, Dept Food & Biotechnol, Sejong 30019, South Korea; Korea Univ, Inst Nat Sci, Sejong 30019, South Korea; Kyungpook Natl Univ, Cell & Matrix Res Inst, Dept Mol Med, Sch Med, Taegu 41566, South Korea; Konkuk Univ, Coll Anim Biosci & Technol, Dept Anim Sci & Technol, Seoul 05029, South Korea | 57215136844; 36602648000; 57221454100; 35242753100; 35074370700; 36067819400; 59051181500 | jinhyuplee@korea.ac.kr;yk46@korea.ac.kr; | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | J MICROBIOL BIOTECHN | 1017-7825 | 1738-8872 | 30 | 1 | SCIE | BIOTECHNOLOGY & APPLIED MICROBIOLOGY;MICROBIOLOGY | 2020 | 2.351 | 72.6 | 1.3 | 2025-06-25 | 19 | 23 | Trehalose; probiotic; reactive oxygen species; programmed cell death | ACUTE DIARRHEA; PREVENTION; APOPTOSIS; GLUTATHIONE; EFFICACY; SAFETY; DAMAGE | Probiotic; Programmed cell death; Reactive oxygen species; Trehalose | Microbial Viability; Oxidative Stress; Probiotics; Saccharomyces boulardii; Trehalose; caspase; caspase YCA1; fungal enzyme; hydrogen peroxide; probiotic agent; reactive oxygen metabolite; trehalose; unclassified drug; trehalose; apoptosis; Article; cell protection; controlled study; cytotoxicity; drug efficacy; fungal viability; fungus culture; nonhuman; oxidative stress; Saccharomyces boulardii; supplementation; drug effect; microbial viability; oxidative stress; Saccharomyces boulardii | English | 2020 | 2020-01 | 10.4014/jmb.1906.06041 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Characteristics and survival impact of polymorphonuclear leucocyte-predominant malignant pleural effusions secondary to lung cancer | Introduction In comparison with mononuclear leucocyte (MNL)-predominant malignant pleural effusions (MPEs), polymorphonuclear leucocyte (PMNL)-predominant MPEs have rarely been investigated and may be associated with a poorer prognosis. Objectives To investigate the characteristics and survival impact of PMNL-predominant MPEs secondary to lung cancer. Methods This retrospective study included patients with MPE secondary to lung cancer, which were classified into the PMNL- and MNL-predominant groups according to cellular predominance in the pleural fluid. Clinical, hematological, radiological, and pleural fluid data were compared between the groups, and the survival impact of PMNL predominance in MPE was evaluated. Results Of the 193 MPEs included, 37 (19%) were characterised by PMNL predominance. Compared to the MNL-predominant group, the PMNL-predominant group showed significantly poorer patient performances (P = .001), higher white blood cell counts (P = .009), higher neutrophil counts, higher blood neutrophil-to-lymphocyte ratio (P = .046), higher serum C-reactive protein (P = .003), lower serum albumin (P < .001), lower pleural fluid pH (P = .002) and higher pleural fluid lactate dehydrogenase (P = .029) levels. In contrast, most clinical and radiological findings, including the duration of symptoms, showed no significant intergroup differences. A shift towards MNL predominance was observed in only 38% of the PMNL-predominant patients who underwent repeat thoracentesis. Overall survival of the PMNL-predominant group was significantly shorter than the MNL-predominant group (P = .003). Conclusions PMNL predominance in MPEs secondary to lung cancer may be observed in variable phases with respect to the duration of symptoms and the time of thoracentesis. Overall, PMNL-predominant MPEs were associated with more advanced stages and poorer survival outcomes, compared to MNL-predominant MPEs. | Lee, Jaehee; Lee, Yong Hoon; Seo, Hyewon; Do, Young Woo; Lee, Deok Heon; Lee, Sang Yub; Lim, Jae Kwang; Yoo, Seung Soo; Lee, Shin Yup; Cha, Seung Ick; Park, Jae Yong; Kim, Chang Ho | Kyungpook Natl Univ, Sch Med, Dept Internal Med, 130 Dongduk Ro, Daegu 41944, South Korea; Kyungpook Natl Univ, Sch Med, Dept Thorac & Cardiovasc Surg, Daegu, South Korea; Kyungpook Natl Univ, Sch Med, Dept Radiol, Daegu, South Korea | Lee, Jun Young/CAI-2335-2022; Lee, Joo/C-3851-2013; Lee, Jaehee/S-1697-2018 | 13805476000; 57199022948; 55612130200; 57189600352; 39561353900; 56016177400; 55515341400; 56479781600; 49863712700; 35227126400; 58360293800; 7409873555 | kimch@knu.ac.kr; | CLINICAL RESPIRATORY JOURNAL | CLIN RESPIR J | 1752-6981 | 1752-699X | 14 | 8 | SCIE | RESPIRATORY SYSTEM | 2020 | 2.57 | 72.7 | 0.21 | 2025-06-25 | 3 | 3 | lung cancer; malignant pleural effusion; polymorphonuclear leucocyte; survival | LYMPHOCYTE RATIO; NEUTROPHILS; FLUID | lung cancer; malignant pleural effusion; polymorphonuclear leucocyte; survival | albumin; C reactive protein; lactate dehydrogenase; adult; aged; albumin blood level; Article; cell survival; controlled study; disease association; disease duration; female; follow up; human; human cell; leukocyte count; lung cancer; major clinical study; male; malignant pleura effusion; neutrophil; neutrophil count; overall survival; pH; pleura fluid; postoperative period; priority journal; protein blood level; retrospective study; thoracocentesis | English | 2020 | 2020-08 | 10.1111/crj.13195 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Emphysema is associated with the aggressiveness of COPD-related adenocarcinomas | Objectives To compare the differences in radiologic and pathologic features of surgically resected chronic obstructive pulmonary disease (COPD)-related adenocarcinomas according to the presence of emphysema. Methods A total of 216 smokers with surgically resected lung adenocarcinoma were included in this retrospective study, and 102 patients were diagnosed with COPD. We classified COPD patients as emphysematous or non-emphysematous group based on the emphysema severity on computed tomography (CT) and evaluated the differences in the CT and pathologic features between the two groups. The relationship between emphysema and disease-free survival was assessed using a Kaplan-Meier curve. Results Lung adenocarcinomas in emphysema group presented a more aggressive pathologic grade and higher prevalence of solid lesions (vs subsolid lesions) on CT than those in non-emphysematous group (P = 0.006 and <0.001, respectively). After adjustment for age, sex, smoking pack-years and tumor size, emphysema group had a greater risk for higher histologic grade and higher prevalence of solid lesions than non-emphysema group (odds ratio, 3.445; 95% confidence interval, 1.124-10.564; P = 0.030, odds ratio, 6.192; 95% confidence interval, 1.804-21.254; P = 0.004, respectively). Kaplan-Meier survival curves showed that patients with emphysema had significantly impaired disease-free survival compared with those without emphysema (median disease-free survival = 37.0 vs 57.5 months, P = 0.038). Conclusion Adenocarcinomas in emphysema-present COPD had more aggressive features of pathology and CT findings, and worse disease-free survival than those without emphysema. These findings might provide an insight into the different pathobiology and prognostic implications of lung adenocarcinomas according to the presence of emphysema in patients with COPD. | Lim, Chun Geun; Shin, Kyung Min; Lim, Jae Kwang; Kim, Hye Jung; Kim, Won Hwa; Cho, Seung Hyun; Kim, Gab Chul; Lim, Jiseun; Jeong, Ji Yun; Cha, Seung-Ick | Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Dept Radiol, Daegu, South Korea; Kyungpook Natl Univ, Chilgok Hosp, Sch Med, Dept Radiol, 807 Hoguk Ro, Daegu 41404, South Korea; Eulji Univ, Dept Prevent Med, Sch Med, Daejeon, South Korea; Kyungpook Natl Univ, Chilgok Hosp, Sch Med, Dept Pathol, Daegu, South Korea; Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Dept Internal Med, Daegu, South Korea | LIM, CHUN GEUN/GXH-5232-2022 | 57195922967; 7402410737; 55515341400; 57203506201; 36081886500; 55686242700; 23004499400; 55630938100; 57205472984; 35227126400 | skmrad@knu.ac.kr; | CLINICAL RESPIRATORY JOURNAL | CLIN RESPIR J | 1752-6981 | 1752-699X | 14 | 4 | SCIE | RESPIRATORY SYSTEM | 2020 | 2.57 | 72.7 | 0.14 | 2025-06-25 | 8 | 6 | adenocarcinoma of lung; COPD; lung neoplasms; pulmonary emphysema | OBSTRUCTIVE PULMONARY-DISEASE; AIR-FLOW OBSTRUCTION; LUNG-CANCER; INTERNATIONAL-ASSOCIATION; IASLC/ATS/ERS CLASSIFICATION; TUMOR RECURRENCE; SURVIVAL; FIBROBLASTS | adenocarcinoma of lung; COPD; lung neoplasms; pulmonary emphysema | Adenocarcinoma; Adenocarcinoma of Lung; Aged; Disease-Free Survival; Female; Humans; Male; Middle Aged; Neoplasm Grading; Pulmonary Disease, Chronic Obstructive; Pulmonary Emphysema; Retrospective Studies; Survival Rate; Tomography, X-Ray Computed; adult; age; aged; aggressiveness; Article; cancer grading; cancer prognosis; cancer surgery; chronic obstructive lung disease; clinical evaluation; clinical feature; comparative study; computer assisted tomography; disease association; disease free survival; disease severity; female; human; lung adenocarcinoma; lung emphysema; major clinical study; male; middle aged; pathology; prevalence; priority journal; radiological procedures; retrospective study; risk factor; sex difference; smoking; survival rate; tumor volume; adenocarcinoma; chronic obstructive lung disease; diagnostic imaging; lung adenocarcinoma; lung emphysema; mortality; pathology; x-ray computed tomography | English | 2020 | 2020-04 | 10.1111/crj.13146 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | A SEARCH FOR EXOPLANETS AROUND NORTHERN CIRCUMPOLAR STARS VI. DETECTION OF PLANETARY COMPANIONS ORBITING THE GIANTS HD 60292 AND HD 112640 | We report the detection of exoplanet candidates in orbits around HD 60292 and HD 112640 from a radial velocity (RV) survey. The stars exhibit RV variations with periods of 495 +/- 3 days and 613 +/- 6 days, respectively. These detections are part of the Search for Exoplanets around Northern Circumpolar Stars (SENS) survey using the fiber-fed Bohyunsan Observatory Echelle Spectrograph installed at the 1.8-m telescope of the Bohyunsan Optical Astronomy Observatory in Korea. The aim of the survey is to search for planetary or substellar companions. We argue that the periodic RV variations are not related to surface inhomogeneities; rather, Keplerian motions of planetary companions are the most likely interpretation. Assuming stellar masses of 1.7 +/- 0.2M(circle dot) (HD 60292) and 1.8 +/- 0.2M(circle dot) (HD 112640), we obtain minimum planetary companion masses of 6.5 +/- 1.0M(Jup) and 5.0 +/- 1.0M(Jup), and periods of 495.4 +/- 3.0 days and 613.2 +/- 5.8 days, respectively. | Lee, Byeong-Cheol; Park, Myeong-Gu; Han, Inwoo; Bang, Tae-Yang; Oh, Hyeong-Il; Choi, Yeon-Ho | Korea Astron & Space Sci Inst, 776 Daedeokdae Ro, Daejeon 34055, South Korea; Univ Sci & Technol, Astron & Space Sci Major, Daejeon 34113, South Korea; Kyungpook Natl Univ, Dept Astron & Atmospher Sci, Daegu 41566, South Korea | 8878262900; 7404490321; 7201559866; 57201317966; 56988390800; 57247914500 | bclee@kasi.re.kr;mgp@knu.ac.kr;iwhan@kasi.re.kr;qkdxodid1230@knu.ac.kr;ymy501@kasi.re.kr;apollo.cho@gmail.com; | JOURNAL OF THE KOREAN ASTRONOMICAL SOCIETY | J KOREAN ASTRON SOC | 1225-4614 | 53 | 1 | SCIE | ASTRONOMY & ASTROPHYSICS | 2020 | 1.39 | 72.8 | 0.14 | 2025-06-25 | 2 | 2 | planetary systems; stars: individual: HD 60292, HD 112640; techniques: radial velocities | K-GIANT; PARAMETERS | HD 112640 — techniques: radial velocities; Planetary systems — stars: individual: HD 60292 | English | 2020 | 2020-02 | 10.5303/jkas.2020.53.1.27 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Bacillus megaterium-induced biocorrosion on mild steel and the effect ofArtemisia pallens methanolic extract as a natural corrosion inhibitor | Methanolic extract ofArtemisia pallens (MEAP) (Asteraceae) was explored as greenbiocorrosion inhibitor for mild steel 1010 in 1.5% sodium chloride environment.Bacillus megateriumSKR7 induces the development of biofilm on the metal surface and forms the pitting corrosion. MEAP was showed (25 ppm) optimum inhibition effect of biocorrosion and further corrosion rate was highly reduced (0.3335 mm/year) than the control system (0.009 mm/year). The electrochemical study has supported the results with a higher value of total resistance (34 omega cm(2)) when compared to control systems. It reveals the formation of a protective layer on the metal surface and reduces the adsorption of biofilm. This was due to the antimicrobial effect of MEAP. Overall, the results recognized that MEAP used as a green corrosion inhibitor for MS 1010 with 83% inhibition efficiency. | Kokilaramani, Seenivasan; AlSalhi, Mohamad S.; Devanesan, Sandhanasamy; Narenkumar, Jayaraman; Rajasekar, Aruliah; Govarthanan, Muthusamy | Thiruvalluvar Univ, Dept Biotechnol, Environm Mol Microbiol Res Lab, Vellore 632115, Tamil Nadu, India; King Saud Univ, Coll Sci, Dept Phys & Astron, POB 2455, Riyadh 11451, Saudi Arabia; Northeastern Univ, Corros & Protect Div, Shenyang Natl Lab Mat Sci, Shenyang 110819, Peoples R China; Kyungpook Natl Univ, Dept Environm Engn, 80 Daehak Ro, Daegu 41566, South Korea | Muthusamy, Govarthanan/C-1491-2014; Govarthanan, Muthusamy/C-1491-2014; Jayaraman, Narenkumar/JGD-7456-2023; alsalhi, mohamad/M-5013-2019; Aruliah, Rajasekar/E-4568-2015; Devanesan, Dr. Sandhanasamy/ABH-3378-2021; Devanesan, Sandhanasamy/ABH-3378-2021; SEENIVASAN, KOKILARAMANI/ADR-9563-2022 | 57217213968; 10440259800; 55279721800; 57194229197; 55928874800; 54881927600 | malsalhi@ksu.edu.sa;rajasekargood@gmail.com;gova.muthu@gmail.com; | ARCHIVES OF MICROBIOLOGY | ARCH MICROBIOL | 0302-8933 | 1432-072X | 202 | 8 | SCIE | MICROBIOLOGY | 2020 | 2.552 | 73.2 | 1.28 | 2025-06-25 | 19 | 25 | Biocorrosion; Mild steel; Bacterial community; Electrochemical studies | CARBON-STEEL; ARTEMISIA-PALLENS; COPPER; WATER; BIOCIDES; SURFACES; BIOFILM; METALS; ACID | Bacterial community; Biocorrosion; Electrochemical studies; Mild steel | Artemisia; Bacillus megaterium; Biofilms; Corrosion; Methanol; Plant Extracts; Steel; Artemisia pallens extract; bacterial DNA; corrosion inhibitor; DNA 16S; organic compound; plant extract; sodium chloride; steel; unclassified drug; methanol; plant extract; adsorption; antimicrobial activity; Artemisia; Artemisia pallens; Article; Bacillus megaterium; bacterial count; bacterial strain; biofilm; controlled study; electrochemical analysis; green chemistry; impedance; microbial corrosion; minimum inhibitory concentration; nonhuman; priority journal; surface property; viable cell count; Artemisia; Bacillus megaterium; chemistry; corrosion; drug effect; metabolism | English | 2020 | 2020-10 | 10.1007/s00203-020-01951-7 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Biocharacteristics and draft genome sequence of Halomonas hydrothermalis C22, a pyruvate-producing halophilic bacterium isolated from a commercial Spirulina culture in Vietnam | Halophilic bacteria are receiving increasing attention for industrial chemical production processes due to their unique properties. Herein, an alkaliphilic and halophilic bacterium was isolated from a commercialSpirulinaculture at Nghe An province in Vietnam and found to secrete pyruvate. Pyruvate is widely used as a starting material in the industrial biosynthesis of pharmaceuticals, and is employed for production of crop protection agents, polymers, cosmetics, and food additives. Phenotypic and chemotaxonomic characterization, and the 16S rRNA gene sequence homology withHalomonas hydrothermalisstrain DSM 15,725 (99.2%) predicted that the strain belongs to theHalomonasgenus, thus we named this strain asH. hydrothermalisstrain C22. We investigated the biocharacteristics and capacity of strain C22 and determined the draft genome sequence comprising 3,934,166 bp with a G + C content of 60.2% encoding 3,668 proteins, 58 tRNAs, 9 rRNAs, and 1 tmRNA. Maximal pyruvate secretion reached 51.1 g/l after 84 h of cultivation. The results will facilitate future studies on the genetic and metabolic diversity of halophilic bacteria and expand our understanding of important bioprocesses in this microorganism. | Hoang Thi Lan Anh; Kawata, Yoshikazu; Luu Thi Tam; Le Thi Thom; Nguyen Cam Ha; Hoang Thi Minh Hien; Ngo Thi Hoai Thu; Pham Quang Nuy; Dang Diem Hong | Vietnam Acad Sci & Technol VAST, Inst Biotechnol IBT, 18 Hoang Quoc Viet, Hanoi, Vietnam; Thuyloi Univ, Hanoi, Vietnam; VAST, Grad Univ Sci & Technol, Hanoi, Vietnam; Natl Inst Adv Ind Sci & Technol, Biomed Res Inst, Osaka, Japan; Kyungpook Natl Univ, Sch Appl Biosci, Daegu, South Korea | Kawata, Yoshikazu/L-7714-2018 | 55256013700; 57197423043; 56052972000; 56649218600; 56053061300; 42161245300; 46661474100; 57214084374; 7201840679 | y-kawata@aist.go.jp;ddhong60vn@yahoo.com; | ARCHIVES OF MICROBIOLOGY | ARCH MICROBIOL | 0302-8933 | 1432-072X | 202 | 5 | SCIE | MICROBIOLOGY | 2020 | 2.552 | 73.2 | 0.07 | 2025-06-25 | 2 | 2 | Draft genome; Halomonas; Halophilic bacteria; Organic acid; Pyruvate | JEOTGALI SP NOV.; MUTANT; KM-1; ACID | Draft genome; Halomonas; Halophilic bacteria; Organic acid; Pyruvate | Base Composition; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Genome, Bacterial; Halomonas; Phylogeny; Pyruvic Acid; RNA, Bacterial; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Spirulina; Vietnam; genomic DNA; pyruvic acid; ribosome RNA; RNA 16S; transfer RNA; bacterial DNA; bacterial RNA; fatty acid; pyruvic acid; ribosome DNA; tmRNA; Article; bacterial genome; bacterium isolation; crop protection; gene sequence; Halomonas; Halomonas hydrothermalis; halophilic bacterium; nonhuman; nucleotide sequence; phylogenetic tree; priority journal; species comparison; Spirulina; Viet Nam; bacterial genome; chemistry; DNA base composition; DNA sequence; genetics; Halomonas; isolation and purification; metabolism; phylogeny; physiology | English | 2020 | 2020-07 | 10.1007/s00203-020-01811-4 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Flaviaesturariibacter flavussp. nov., isolated from soil in Jeju Island | A Gram-stain-negative, non-motile and yellow-colored bacterium, designated 17J68-12(T), was isolated from soil in Jeju Island, Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 17J68-12(T)formed a distinct lineage within the familyChitinophagaceaeand was mostly related to members ofFlaviaesturariibacter luteus(97.5%),Flaviaesturariibacter amylovorans(96.8%) andFlaviaesturariibacter terrae(96.8%). Growth was observed at 18-42 degrees C (optimum 30 degrees C) in R2A broth at pH 7.0. The major cellular fatty acids of the strain 17J68-12( T)were summed feature 3 (C-16:1 omega 6cand/or C-16:1 omega 7c), summed feature 1 (C(15:1)iso-H and/or C(13:0)3-OH), and iso-C-15:0. The predominant respiratory quinones are MK-7 and MK-6. The major polar lipid was identified as phosphatidylethanolamine. Based on biochemical, chemotaxonomic and phylogenetic characteristics, the strain 17J68-12(T)represents a novel bacterial species within the familyChitinophagaceae, for which the nameFlaviaesturariibacter flavussp. nov. is proposed. The type strain ofFlaviaesturariibacter flavusis 17J68-12(T)(= KCTC 62219(T) = JCM 33179(T)). | Jang, Jun Hwee; Maeng, Soo Hyun; Jung, Hee Young; Kim, Myung Kyum; Subramani, Gayathri | Seoul Womens Univ, Coll Nat Sci, Dept Bio & Environm Technol, Seoul 01797, South Korea; Kyungpook Natl Univ, Coll Agr & Life Sci, Daegu 41566, South Korea | Kim, Jung Oh/JDC-5061-2023; Subramani, Gayathri/AGF-7052-2022 | 57201467227; 57205512087; 7403029383; 35080583500; 57205424880 | biotech@swu.ac.kr;drgaya@swu.ac.kr; | ARCHIVES OF MICROBIOLOGY | ARCH MICROBIOL | 0302-8933 | 1432-072X | 202 | 10 | SCIE | MICROBIOLOGY | 2020 | 2.552 | 73.2 | 0.07 | 2025-06-25 | 1 | 1 | Chitinophagaceae; Flaviaesturariibacter; Novel species; Taxonomy | GEN. NOV.; BACTERIUM | Chitinophagaceae; Flaviaesturariibacter; Novel species; Taxonomy | Bacterial Typing Techniques; Bacteroidetes; Base Composition; DNA, Bacterial; Fatty Acids; Phylogeny; Republic of Korea; RNA, Ribosomal, 16S; Soil Microbiology; Species Specificity; alpha glucosidase; bacterial DNA; bacterial RNA; chymotrypsin A; esterase; fatty acid; genomic DNA; phosphatidylethanolamine; quinone derivative; RNA 16S; trypsin; bacterial DNA; fatty acid; RNA 16S; Article; bacterial genome; bacterial growth; bacterial strain; biochemical analysis; biotechnological production; chemotaxonomy; controlled study; enzyme activity; Flaviaesturariibacter flavus; Gram negative bacterium; island (geological); lipid fingerprinting; maximum likelihood method; new species; nonhuman; nucleotide sequence; phylogeny; priority journal; RNA sequence; soil microflora; South Korea; bacterium identification; Bacteroidetes; classification; DNA base composition; genetics; isolation and purification; microbiology; phylogeny; species difference | English | 2020 | 2020-12 | 10.1007/s00203-020-01963-3 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | In vitro screening and in silico prediction of antifungal metabolites from rhizobacteriumAchromobacter kerstersiiJKP9 | The main objective of this study was to identify the antifungal metabolites fromAchromobacter kerstersiiJKP9, a rhizosphere bacterium isolated from tomato cultivations, inhibiting the melanin biosynthetic pathways in vascular wilt pathogenFusarium oxysporumf. sp.lycopersici(Fol).To achieve this objective, all the rhizobacterial morphotypes were screened for plant-growth-promoting and antagonistic activities. Ethyl acetate extract ofAchromobacter kerstersiiJKP9 was purified in HPLC and predicted for antifungals in GC-MS equipped with Wiley library. After identification, molecular docking of useful ligands with modeled Short-chain Dehydrogenase/ Reductase (SDR) of Fol (Locus: FOXG₀₀₄₇₂). Results were indicated that the potential strainAchromobacter kerstersiiJKP9 exclusively secreted five pyrrole analogs notable for their antifungal role with no extracellular antifungal enzyme production as seen in other rhizobacterial isolates. In silico docking studies identified, Pyrrolo[1, 2-a]pyrazine-1,4-dione, hexahydro- as effective for SDR in Fol. From these results, we conclude that bacterial pyrroles can be used as an effective fungicide to controlFusariumwilt in tomatoes. In the future, these pyrrole derivatives can directly be employed as eco-friendly fungicides or may be used as antifungal supplements in agrochemical products for the sustainable production of tomatoes. | Vijay, Karuppiah; Devi, Thangarasu Suganya; Sree, Karthikeyan Kirupa; Elgorban, Abdallah M.; Kumar, Ponnuchamy; Govarthanan, Muthusamy; Kavitha, Thangavel | Alagappa Univ, Dept Microbiol, Sci Campus, Karaikkudi, Tamil Nadu, India; King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh 11451, Saudi Arabia; Alagappa Univ, Dept Anim Hlth & Management, Sci Campus, Karaikkudi, Tamil Nadu, India; Kyungpook Natl Univ, Dept Environm Engn, Daegu 41566, South Korea | ; Ponnuchamy, Kumar/D-3470-2013; Elgorban, Dr. Abdallah/C-6000-2013; Govarthanan, Muthusamy/C-1491-2014; Karuppiah, Vijay/GMW-9090-2022; Muthusamy, Govarthanan/C-1491-2014 | 57216493201; 57216491230; 57216487706; 55353581000; 55173720800; 54881927600; 57214889652 | kavikmicro@gmail.com; | ARCHIVES OF MICROBIOLOGY | ARCH MICROBIOL | 0302-8933 | 1432-072X | 202 | 10 | SCIE | MICROBIOLOGY | 2020 | 2.552 | 73.2 | 0.81 | 2025-06-25 | 16 | 16 | Antifungals; Achromobacter kerstersii; Pyrroles; Rhizosphere bacteria; Vascular wilt | PLANT-GROWTH; FUSARIUM-WILT; STRUCTURAL-CHARACTERIZATION; PYRROLE DERIVATIVES; MOLECULAR DOCKING; BACTERIA; EXTRACT; XYLOSOXYDANS; PURIFICATION; PROMOTION | Achromobacter kerstersii; Antifungals; Pyrroles; Rhizosphere bacteria; Vascular wilt | Achromobacter; Antifungal Agents; Fungicides, Industrial; Fusarium; Lycopersicon esculentum; Molecular Docking Simulation; Plant Diseases; Pyrroles; acetic acid ethyl ester; antifungal agent; bacterial extract; fungicide; ligand; pyrrole derivative; pyrrolol[1,2 a]pyrazine 1,4 dione; short chain dehydrogenase/reductase; unclassified drug; antifungal agent; fungicide; pyrrole derivative; Achromobacter kerstersii; Article; bacterial strain; computer model; computer prediction; controlled study; enzyme synthesis; Fusarium wilt; gene locus; high performance liquid chromatography; in vitro study; mass fragmentography; molecular docking; morphotype; nonhuman; plant growth-promoting bacterium; priority journal; rhizosphere bacterium; screening; solvent extraction; tomato; Achromobacter; drug effect; Fusarium; genetics; metabolism; microbiology; plant disease; prevention and control | English | 2020 | 2020-12 | 10.1007/s00203-020-01982-0 | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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