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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | ○ | Article | Cutibacterium acnes in Shoulder Surgery: Is It a Significant Risk Factor for Postoperative Infection? | Cutibacterium acnes, a commensal, lipophilic, anaerobic Gram-positive bacterium, is well known for its potential to cause infections, particularly in the field of orthopedics, notably in the shoulder. However, its indolent strain nature presents challenges in the diagnosis of the bacterium using clinical, laboratory, and culture-based methods. There are controversies surrounding its actual threat as an infection-causing agent, leading to an incomplete consensus on treatment strategies after the infection. Furthermore, research is ongoing to explore preventive procedures before the onset of infection. This review aimed to comprehensively explore the diagnosis and treatment of C. acnes and determine whether it is a risk factor for shoulder joint infections. | Yoon, Jong Pil; Lee, Kang-San; Park, Sung-Jin; Kim, Dong-Hyun; Kim, Junsung; Choi, Yoon Seong; Lee, Hyun Joo; Chung, Seok Won | Kyungpook Natl Univ Hosp, Dept Orthoped Surg, 130 Dongdeok Ro, Daegu, South Korea; Kyungpook Natl Univ, Dept Carbon Hybrid Fiber Sci, Seoul, South Korea; Konkuk Univ Med Ctr, Dept Orthoped Surg, Seoul, South Korea | 36098548400; 58967020700; 58527890700; 57198637188; 58527890900; 57013770300; 58838750100; 37065938600 | lksan202@gmail.com; | CLINICS IN ORTHOPEDIC SURGERY | CLIN ORTHOP SURG | 2005-291X | 2005-4408 | 16 | 6 | SCIE | ORTHOPEDICS | 2024 | 2 | 44.2 | 0 | 2025-05-07 | 0 | 0 | Cutibacterium acnes; Shoulder joint; Surgical site infection | PROSTHETIC-JOINT INFECTION; PROPIONIBACTERIUM-ACNES; BENZOYL PEROXIDE; SERUM INTERLEUKIN-6; REVISION; ARTHROPLASTY; DIAGNOSIS; CULTURE; EFFICACY; HIP | Cutibacterium acnes; Shoulder joint; Surgical site infection | Gram-Positive Bacterial Infections; Humans; Propionibacteriaceae; Propionibacterium acnes; Risk Factors; Shoulder Joint; Surgical Wound Infection; acne; commensal; diagnosis; drug therapy; female; human; infectious arthritis; nonhuman; postoperative infection; Propionibacterium acnes; review; risk factor; shoulder; shoulder joint; shoulder surgery; surgery; surgical infection; Gram positive infection; microbiology; prevention and control; Propionibacteriaceae; Propionibacterium acnes; risk factor; surgical infection | English | 2024 | 2024-12 | 10.4055/cios23371 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Efficacy beliefs and motivational dynamics in early-season collegiate swimming relays | This study examined the moderating effects of efficacy beliefs and perceived indispensability on individual efforts in early-season collegiate swimming relays. Effort changes from swimmers' individual best times to relay-leg times were compared as a function of relay members' relative strength (i.e., fastest or slowest) and serial position (i.e., first or last leg). Participants (N = 199) were collegiate swimmers in 200, 400, or 800-yard freestyle relays at fall invitational meets providing 260 cases included for analysis. Individual lifetime best times, adjusted relay times, rank, serial position, and self-report measures (i.e., efficacy, perceived indispensability of their relay performance) were used for hierarchical multiple regressions. Results indicated that the slowest relay members came closer to their individual best times compared with their team members. The fastest members showed less effort change toward their individual best times than their team members. However, efficacy beliefs in one's teammates had a small positive moderating influence on the fastest members' effort in relay performances. Based on our results, we recommend Division II and III collegiate coaches not be too concerned with positional order by particular rank at early season invitationals; instead, focus on how to obtain the most effort from each relay member. However, the slowest swimmers may perform better if not positioned first. | Hwang, Seunghyun; Sedabres, Kaitlynn; Dithurbide, Lori; Feltz, Deborah | Kyungpook Natl Univ, Dept Leisure Sport, Daegu, South Korea; Michigan State Univ, Dept Kinesiol, E Lansing, MI USA; Dalhousie Univ, Sch Hlth & Human Performance, Halifax, NS, Canada; Kyungpook Natl Univ, Dept Leisure Sport, 2559 Gyeongsang Daero, Gyeongsangbuk Do, South Korea | ; Feltz, Deborah/AAX-6575-2020 | 55571144800; 58159104000; 35068141200; 7003458695 | hwangsh@knu.ac.kr; | INTERNATIONAL JOURNAL OF SPORTS SCIENCE & COACHING | INT J SPORTS SCI COA | 1747-9541 | 2048-397X | 19 | 2 | SSCI | HOSPITALITY, LEISURE, SPORT & TOURISM;PSYCHOLOGY, APPLIED | 2024 | 2.1 | 44.2 | 0 | 2025-05-07 | 1 | 0 | Collective effort; Kohler effect; perceived indispensability; social comparison; social loafing | SELF-EFFICACY; EFFORT GAINS; LOSSES | Collective effort; Köhler effect; perceived indispensability; social comparison; social loafing | English | 2024 | 2024-04 | 10.1177/17479541231165133 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Staged Fixation with Respect to Soft Tissue in Tibial Plateau Fractures with Acute Compartment Syndrome: Correlation Analysis of Complications | Background: Staged operations are commonly employed in the management of high-energy tibial plateau fractures (TPF) complicated by acute compartment syndrome (ACS); however, complications, such as nonunion, deep wound infection, and traumatic arthritis, often occur due to severe bone and soft-tissue damage. We aimed to report the radiological and clinical outcomes of staged surgical interventions performed following complete closure of the fasciotomy wound for the treatment of TPF complicated by ACS. Additionally, we analyzed factors associated with complications arising from these procedures. Methods: Thirty patients with TPF and ACS were included (23 men and 7 women; average age, 59.7 years). The mean followup period was 33.2 months (range, 12-85 months). An external fixator was initially applied with emergency fasciotomy, and open reduction and plate fixation were performed after complete closure of the fasciotomy wound and soft-tissue stabilization (mean, 31 days; range, 9-55 days). Radiological evaluation of bone union and alignment was conducted, functional evaluation of the knee and ankle joints was performed using the Knee Society and American Orthopedic Foot and Ankle Society (AOFAS) scores, and complications and related factors were analyzed. Results: Primary bone union was achieved in 29 of the 30 cases (96.7%) at an average of 20.8 weeks (range, 12-35 weeks). Malalignment was not observed in any case. At the final follow-up examination, the mean Knee Society and AOFAS scores were 92.5 (range, 65-100) and 95.5 (range, 74-100), respectively. Complications included 1 case of nonunion (3.3%), 2 cases of deep wound infection (6.7%), and 5 cases of traumatic arthritis (16.7%). A statistically significant correlation was noted between complications and patients who underwent dual approaches for the fixation of bicondylar TPFs. Conclusions: A staged operation coupled with sufficient soft-tissue healing can achieve excellent bone union and functional outcomes in patients with TPF and ACS. However, complications may occur more often in patients undergoing dual approaches for bicondylar TPFs, necessitating vigilant monitoring and management. | Yoon, Yong-Cheol; Kim, Ye Joon; Oh, Chang-Wug; Kim, Hee-June; Sim, Seung-Bo; Son, Sang-Woo; Kim, Joon-Woo | Gachon Univ, Coll Med, Trauma Ctr, Orthoped Trauma Div, Incheon, South Korea; Denver Hlth Med Ctr, Dept Orthoped, Denver, CO USA; Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Dept Orthoped Surg, 130 Dongdeok Ro, Daegu 41944, South Korea | Yong-Cheol, Yoon/HPD-8077-2023; Oh, Chang-Wug/AAO-4602-2021 | 37082544400; 57211919186; 22135834200; 36463735200; 59574988400; 59629229300; 22951329000 | joonwoo@knu.ac.kr; | CLINICS IN ORTHOPEDIC SURGERY | CLIN ORTHOP SURG | 2005-291X | 2005-4408 | 16 | 6 | SCIE | ORTHOPEDICS | 2024 | 2 | 44.2 | 0.92 | 2025-05-07 | 1 | 1 | Tibial plateau fracture; Compartment syndrome; Soft tissue injuries; External fixator; Complications | INFECTION; MANAGEMENT | Compartment syndrome; Complications; External fixator; Soft tissue injuries; Tibial plateau fracture | Adult; Aged; Compartment Syndromes; External Fixators; Fasciotomy; Female; Fracture Fixation; Fracture Fixation, Internal; Humans; Male; Middle Aged; Postoperative Complications; Retrospective Studies; Tibial Fractures; Tibial Plateau Fractures; acute compartment syndrome; adult; Article; clinical article; clinical feature; clinical outcome; correlational study; emergency surgery; fasciotomy; female; follow up; fracture fixation; fracture healing; fracture nonunion; functional status; human; Knee Society and American Orthopedic Foot and Ankle Society score; male; middle aged; open fracture reduction; plate fixation; posttraumatic arthropathy; retrospective study; risk factor; scoring system; soft tissue; surgical infection; tibial plateau fracture; adverse event; aged; compartment syndrome; complication; etiology; external fixator; fasciotomy; fracture fixation; osteosynthesis; postoperative complication; procedures; surgery; tibia fracture; tibial plateau fracture | English | 2024 | 2024-12 | 10.4055/cios24058 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Tissue-Engineered Bone Regeneration for Medium-to-Large Osteonecrosis of the Femoral Head in the Weight-Bearing Portion: An Observational Study | Background: Stem cell therapy for the treatment of osteonecrosis of the femoral head (ONFH) showed promising outcomes. However, ONFH with a large lesion in the weight-bearing portion is a poor prognostic factor and still challenging issue to be solved. We aimed to evaluate the effect of tissue-engineered bone regeneration for this challenging condition to preserve the femoral head. Methods: A total of 7 patients (9 hips) with ONFH who received osteoblasts expanded ex vivo from bone marrow-derived mesenchymal stem cells (BMdMSCs) and calcium metaphosphate (CMP) as scaffolds from March 2002 to March 2004 were retrospectively reviewed. The median age was 27.0 years (interquartile range [IQR], 23.0-34.0 years), and the median follow-up period was 20.0 years (IQR, 11.0-20.0 years). After culture and expansion of stem cells, we performed core decompression with BMdMSC implantation at a median number of 10.1 x107 (IQR, 9.9-10.9 x107). To evaluate radiographic outcomes, the Association Research Circulation Osseous (ARCO) classifications, the Japanese Investigation Committee (JIC) classification, and modified Kerboul combined necrotic angle (mKCNA) were evaluated preoperatively and during follow-up. Clinical outcomes were evaluated by a visual analog scale (VAS) and Harris Hip Score (HHS). Results: The preoperative stage of ONFH was ARCO 2 in 5 hips and ARCO 3a in 4 hips. The ARCO staging was maintained in 3 hips of ARCO 2 and 4 hips of ARCO 3a. Two hips of ARCO 2 with radiographic progression underwent total hip arthroplasty. According to mKCNA, 2 hips showed medium lesions, and 7 hips showed large lesions. The size of necrotic lesion was decreased in 4 hips (2 were ARCO 2 and 2 were ARCO 3a). There were no significant changes in JIC classification in all hips (type C1: 3 hips and type C2: 6 hips) (p = 0.655). Clinically, there were no significant changes in the VAS and HHS between preoperative and last followup (p = 0.072 and p = 0.635, respectively). Conclusions: Tissue engineering technique using osteoblasts expanded ex vivo from BMdMSC and CMP showed promising outcomes for the treatment of pre-collapsed and early-collapsed stage ONFH with medium-to-large size, mainly located in weight- bearing areas. | Park, Eui-Kyun; Shim, Bum-Jin; Kim, Suk-Young; Baek, Seung-Hoon; Kim, Shin-Yoon | Kyungpook Natl Univ, Coll Dent, Dept Pathol & Regenerat Med, Daegu, South Korea; Kyungpook Natl Univ Chilgok Hosp, Dept Orthoped Surg, Daegu, South Korea; Yeungnam Univ, Sch Mat Sci & Engn, Gyongsan, South Korea; Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Coll Med, Dept Orthoped Surg, 130 Dongdeok Ro, Daegu 41944, South Korea; Yeungnam Univ, Yeungnam Univ Hosp, Dept Orthoped Surg, Coll Med, Daegu, South Korea | 37071072400; 57201499293; 55849842100; 56232924900; 26663842900 | syukim@knu.ac.kr; | CLINICS IN ORTHOPEDIC SURGERY | CLIN ORTHOP SURG | 2005-291X | 2005-4408 | 16 | 5 | SCIE | ORTHOPEDICS | 2024 | 2 | 44.2 | 0.92 | 2025-05-07 | 1 | 1 | Femoral head; Osteonecrosis; Osteoblast; Stem cell; Tissue engineered | STEM-CELL THERAPY; CORE DECOMPRESSION; AVASCULAR NECROSIS; FOLLOW-UP; COLLAPSE; IMPLANTATION; PREDICTION; HIP | Femoral head; Osteoblast; Osteonecrosis; Stem cell; Tissue engineered | Adult; Bone Regeneration; Female; Femur Head Necrosis; Humans; Male; Mesenchymal Stem Cell Transplantation; Osteoblasts; Retrospective Studies; Tissue Engineering; Tissue Scaffolds; Weight-Bearing; Young Adult; adolescent; adult; Article; bone marrow mesenchymal stem cell transplantation; bone regeneration; cell expansion; clinical article; clinical outcome; ex vivo study; female; femur head necrosis; follow up; Harris hip score; hip radiography; human; human cell; male; observational study; osteoblast; preoperative period; retrospective study; tissue engineering; total hip replacement; visual analog scale; weight bearing; young adult; femur head necrosis; mesenchymal stem cell transplantation; physiology; procedures; surgery; tissue scaffold | English | 2024 | 2024-10 | 10.4055/cios23344 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | A Novel Truncated CHAP Modular Endolysin, CHAPSAP26-161, SAP26-161, That Lyses Staphylococcus aureus, , Acinetobacter baumannii, , and Clostridioides difficile, , and Exhibits Therapeutic Effects in a Mouse Model of A. baumannii Infection | Development of novel antibacterial agents is imperative due to the increasing threat of antibiotic- resistant pathogens. This study aimed to develop the enhanced antibacterial activity and in- vivo efficacy of a novel truncated endolysin, CHAPSAP26-161, SAP26-161, derived from the endolysin LysSAP26, against multidrug-resistant bacteria. CHAPSAP26-161 SAP26-161 exhibited higher protein purification efficiency in E. coli and antibacterial activity than LysSAP26. Moreover, CHAPSAP26-161 SAP26-161 showed the higher lytic activity against A. baumannii with minimal bactericidal concentrations (MBCs) of 5-10 mu g/ml, followed by Staphylococcus aureus with MBCs of 10-25 mu g/ml. Interestingly, CHAPSAP26-161 SAP26-161 could lyse anaerobic bacteria, such as Clostridioides difficile, , with MBCs of 25-50 mu g/ml. At pH 4-8 and temperatures of 4oC-45oC, o C-45 o C, CHAPSAP26-161 SAP26-161 maintained antibacterial activity without remarkable difference. The lytic activity of CHAPSAP26-161 SAP26-161 was increased with Zn2+. 2+ . In vivo tests demonstrated the therapeutic effects of CHAPSAP26-161 SAP26-161 in murine systemicA. A. baumannii infection model. In conclusion, CHAPSAP26-161, SAP26-161, a truncated endolysin that retains only the CHAP domain from LysSAP26, demonstrated enhanced protein purification efficiency and antibacterial activity compared to LysSAP26. It further displayed broad-spectrum antibacterial effects against S. aureus, , A. baumannii, , and C. difficile. . Our in vitro and in-vivo results of CHAPSAP26-161 SAP26-161 highlights its promise as an innovative therapeutic option against those bacteria with multiple antibiotic resistance. | Choi, Yoon-Jung; Kim, Shukho; Dahal, Ram Hari; Kim, Jungmin | Kyungpook Natl Univ, Sch Med, Dept Microbiol, Daegu 41566, South Korea | Dahal, Ram Hari/H-8673-2016 | 57220082311; 24341187900; 57110097800; 57211297681 | minkim@knu.ac.kr; | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | J MICROBIOL BIOTECHN | 1017-7825 | 1738-8872 | 34 | 8 | SCIE | MICROBIOLOGY;BIOTECHNOLOGY & APPLIED MICROBIOLOGY | 2024 | 3.1 | 44.4 | 0.19 | 2025-05-07 | 2 | 2 | Endolysin; deletion mutants; resistance; Acinetobacter baumannii; Staphylococcus aureus | BACTERIOPHAGE ENDOLYSINS; PROTEIN; DOMAIN | Acinetobacter baumannii; deletion mutants; Endolysin; resistance; Staphylococcus aureus | Acinetobacter baumannii; Acinetobacter Infections; Animals; Anti-Bacterial Agents; Clostridioides difficile; Disease Models, Animal; Drug Resistance, Multiple, Bacterial; Endopeptidases; Escherichia coli; Female; Hydrogen-Ion Concentration; Mice; Mice, Inbred BALB C; Microbial Sensitivity Tests; Staphylococcal Infections; Staphylococcus aureus; Temperature; zinc ion; antiinfective agent; endolysin; proteinase; Acinetobacter baumannii; anaerobic bacterium; animal experiment; animal model; animal tissue; antibacterial activity; antibiotic resistance; Clostridioides difficile; controlled study; deletion mutant; drug comparison; Escherichia coli; in vitro study; lysis; minimum bactericidal concentration; mouse; mouse model; nonhuman; pH; protein purification; review; Staphylococcus aureus; therapy effect; Acinetobacter infection; animal; Bagg albino mouse; chemistry; disease model; drug effect; drug therapy; female; genetics; metabolism; microbial sensitivity test; microbiology; multidrug resistance; Staphylococcus infection; temperature | English | 2024 | 2024-08 | 10.4014/jmb.2402.02042 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | A single-blind randomised study comparing the efficacy of fluconazole and itraconazole for the treatment of Malassezia dermatitis in client-owned dogs | Background: No reports have compared the clinical therapeutic efficacy of fluconazole and itraconazole in canine Malassezia dermatitis.Objectives: The study aimed to compare the clinical therapeutic efficacy of fluconazole and itraconazole and to evaluate the adverse effects of fluconazole in canine Malassezia dermatitis.Animals: Sixty-one client-owned dogs with Malassezia dermatitis.Materials and Methods: The enrolled animals were randomly divided into groups receiving 5 mg/kg fluconazole (5FZ), 10 mg/kg fluconazole (10FZ) or 5 mg/kg itraconazole (5IZ). The drugs were orally administered once daily for 28 days. Cytological examination, clinical index score (CIS), pruritus Visual Analog Scale (PVAS) evaluation and blood analysis (for 5FZ only) were performed on Day (D)0, D14 and D28.Results: On D14, significant reductions in mean yeast count (MYC), CIS and PVAS were observed in the 5FZ (n = 20, p < 0.01), 10FZ (n = 17, p < 0.01) and 5IZ (n = 16, p < 0.05) groups. In all three groups, a significant reduction (p < 0.001) in MYC, CIS and PVAS expression was observed on D28. There was no significant difference in the percentage reduction of MYC, CIS and PVAS among the groups. Moreover, there was a significant difference (p < 0.05) in each group between D14 and D28, except for the percentage reduction in MYC in the 10FZ and 5IZ groups. No adverse effects of fluconazole were observed in the 5FZ or 10FZ groups.Conclusions and Clinical Relevance: This study indicates that 5FZ and 10FZ are as effective as itraconazole in canine Malassezia dermatitis. | Lee, Hayoon; Koo, Yoonhoi; Yun, Taesik; Lee, Dohee; Chae, Yeon; Min, Kyung-Duk; Kim, Hakhyun; Yang, Mhan-Pyo; Kang, Byeong-Teck | Chungbuk Natl Univ, Coll Vet Med, Lab Vet Internal Med & Dermatol, Cheongju, Chungbuk, South Korea; Kyungpook Natl Univ, Coll Vet Med, Daegu, South Korea; Chungbuk Natl Univ, Coll Vet Med, Lab Vet Surg, Cheongju, Chungbuk, South Korea; Chungbuk Natl Univ, Coll Vet Med, Lab Vet Internal Med & Dermatol, Cheongju 28644, Chungbuk, South Korea | 58062005000; 57220050745; 57209458067; 57226189094; 57215577118; 57218662067; 57192503045; 7404927049; 8858992400 | kangbt@chungbuk.ac.kr; | VETERINARY DERMATOLOGY | VET DERMATOL | 0959-4493 | 1365-3164 | 35 | 3 | SCIE | DERMATOLOGY;VETERINARY SCIENCES | 2024 | 1.4 | 44.4 | 0 | 2025-05-07 | 0 | 0 | clinical efficacy; fluconazole; itraconazole; Malassezia dermatitis | ATOPIC-DERMATITIS; CONTROLLED-TRIAL; PACHYDERMATIS; TRIAZOLE | clinical efficacy; fluconazole; itraconazole; Malassezia dermatitis | Animals; Antifungal Agents; Dermatomycoses; Dog Diseases; Dogs; Female; Fluconazole; Itraconazole; Malassezia; Male; Single-Blind Method; Treatment Outcome; alanine aminotransferase; albumin; alkaline phosphatase; aspartate aminotransferase; creatinine; fluconazole; itraconazole; antifungal agent; fluconazole; itraconazole; animal experiment; Article; blood analysis; blood sampling; body weight; clinical index score; clinical evaluation; controlled study; cytology; dog; drug efficacy; hematocrit; leukocyte count; Malassezia dermatitis; nonhuman; platelet count; prophylaxis; randomized controlled trial; scoring system; single blind procedure; urea nitrogen blood level; vein puncture; visual analog scale; animal; comparative study; dermatomycosis; dog disease; drug effect; female; Malassezia; male; microbiology; treatment outcome; veterinary medicine | English | 2024 | 2024-06 | 10.1111/vde.13233 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Anti-Inflammatory Effect of Extract from Fragaria ananassa Duch. Calyx via MAPK and NF-κB Signaling Pathway | Currently, Fragaria ananassa Duch. are discarded as by-products except for the fruit part, so we developed a natural material using the top (= calyx), one of the by-products, and prepared an extract using 70% ethanol to investigate its effects on anti-inflammatory mechanisms. The polyphenol content of 70% ethanol extracts from Fragaria ananassa Duch. calyx was measured to be 265.86 +/- 0.85 mg TAE/100g, respectively. The antioxidant activity was confirmed through the electron donating ability and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) radical scavenging ability measurements. When extracts from Fragaria ananassa Duch. calyx was treated to LPSinduced RAW 264.7 cells, it was confirmed that the production of inflammation-related factors, NO, PGE2, iNOS, COX-2, TNF-a, and IL-6, was inhibited. In addition, it was confirmed that extracts from Fragaria ananassa Duch. calyx affected the MAPK signaling pathway by reducing the protein expression of p-ERK, p-JNK, and p-p38, which are the upper signaling pathways. In addition, it was confirmed to reduce the protein expression of p-p65 and p-I kappa B, which are NF-kappa B signaling pathways. Therefore, this study suggests that extracts from Fragaria ananassa Duch. calyx affect the regulation of the production of major inflammation-related factors by inhibiting the MAPK and NF-kappa B signaling pathway. These results confirmed that extracts from Fragaria ananassa Duch. calyx have the potential to be developed as a new natural material with anti-inflammatory activity. | Kim, Hyo-Min; Yoo, Dan-Hee; Kang, Jung-Wook; Lee, In-Chul; Bae, Jong-Sup | Kyungpook Natl Univ, Coll Pharm, Daegu 41566, South Korea; Seowon Univ, Coll Fus & Convergence, Cheongju 28674, South Korea; Seowon Univ, Dept Biocosmet Sci, Cheongju 28674, South Korea | 57581209400; 57189321512; 57678656700; 56390536600; 16021543200 | 5229418@hanmail.net;baejs@knu.ac.kr; | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | J MICROBIOL BIOTECHN | 1017-7825 | 1738-8872 | 34 | 12 | SCIE | MICROBIOLOGY;BIOTECHNOLOGY & APPLIED MICROBIOLOGY | 2024 | 3.1 | 44.4 | 0 | 2025-05-07 | 0 | 0 | Fragaria ananassa Duch.; natural material; anti-inflammatory; MAPK; NF-kappa B | RAW 264.7 MACROPHAGES; INDUCED INOS; TNF-ALPHA; LIPOPOLYSACCHARIDE; INACTIVATION; INHIBITION; EXPRESSION; STRAWBERRY; COX-2; IL-6 | anti-inflammatory; Fragaria ananassa Duch.; MAPK; natural material; NF-κB | Animals; Anti-Inflammatory Agents; Antioxidants; Cyclooxygenase 2; Dinoprostone; Fragaria; Inflammation; Interleukin-6; Lipopolysaccharides; Macrophages; MAP Kinase Signaling System; Mice; Mitogen-Activated Protein Kinases; NF-kappa B; Nitric Oxide; Nitric Oxide Synthase Type II; Plant Extracts; Polyphenols; RAW 264.7 Cells; Signal Transduction; Tumor Necrosis Factor-alpha; alcohol; antiinflammatory agent; ascorbic acid; cyclooxygenase 2; I kappa B; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; interleukin 6; lipopolysaccharide; messenger RNA; mitogen activated protein kinase; nitric oxide; plant extract; polyphenol; prostaglandin E2; strawberry extract; stress activated protein kinase; transcription factor RelA; tumor necrosis factor; unclassified drug; antioxidant; cyclooxygenase 2; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; interleukin 6; mitogen activated protein kinase; nitric oxide; plant extract; polyphenol; prostaglandin E2; tumor necrosis factor; ABTS radical scavenging assay; antiinflammatory activity; antioxidant activity; Article; cell viability; controlled study; cytokine production; DPPH radical scavenging assay; drug mechanism; enzyme phosphorylation; gene expression; IC50; macrophage; MAPK signaling; NF kB signaling; prostaglandin synthesis; protein expression; RAW 264.7 cell line; strawberry; animal; chemistry; drug effect; Fragaria; inflammation; MAPK signaling; metabolism; mouse; signal transduction | English | 2024 | 2024-12 | 10.4014/jmb.2409.09044 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Bacterial Apoptosis-Like Death through Accumulation of Reactive Oxygen Species Quercetin in Escherichia coli | The antimicrobial activity of the natural compounds from plant and food have well discovered since the interest on the beneficial effect of the natural compounds was risen. Quercetin, a flavonoid derived from vegetables, including onions, red leaf lettuces and cherries has been studied for diverse biological characteristics as anti-cancer and anti-microbial activities. The aim of current study is to investigate the specific antibacterial modes of action of quercetin against Escherichia coli. Quercetin decreased the E. coli cell viability and induced the severe damages (oxidative stress, DNA fragmentation) leading to cell death. Reactive oxygen species (ROS) generation was observed during the process, which we confirmed that oxidative stress was the key action of antibacterial activity of quercetin exerting its influence potently. Based on the results of Annexin V and Caspace FITC-VAD-FMK assay, the oxidative damage in E. coli has led to the bacterial apoptosis-like death in E. coli. To sum up, the contribution of ROS generation exerts crucial impact in antibacterial activity of quercetin. | Kwun, Min Seok; Lee, Dong Gun | Kyungpook Natl Univ, Coll Nat Sci, Sch Life Sci, BK 21 FOUR KNU Creat Biores Grp, Daegu 41566, South Korea | Lee, Dong-Gun/IWD-9833-2023 | 57212491555; 55668060900 | dglee222@knu.ac.kr; | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | J MICROBIOL BIOTECHN | 1017-7825 | 1738-8872 | 34 | 7 | SCIE | MICROBIOLOGY;BIOTECHNOLOGY & APPLIED MICROBIOLOGY | 2024 | 3.1 | 44.4 | 1.39 | 2025-05-07 | 3 | 3 | Apoptosis-like death; Escherichia coli; quercetin; reactive oxygen species | OXIDATIVE STRESS; ANTIOXIDANT | Apoptosis-like death; Escherichia coli; quercetin; reactive oxygen species | Anti-Bacterial Agents; Apoptosis; DNA Fragmentation; Escherichia coli; Microbial Sensitivity Tests; Microbial Viability; Oxidative Stress; Quercetin; Reactive Oxygen Species; fluorescein isothiocyanate; lipocortin 5; quercetin; reactive oxygen metabolite; antiinfective agent; quercetin; reactive oxygen metabolite; antibacterial activity; apoptosis; Article; bacterial strain; cell viability; controlled study; DNA fragmentation; Escherichia coli; nonhuman; oxidative stress; drug effect; Escherichia coli; metabolism; microbial sensitivity test; microbial viability; oxidative stress | English | 2024 | 2024-07 | 10.4014/jmb.2403.03057 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Bacterial Community of Breast Milk in Breastfeeding Women Using Culture-Dependent and Culture-Independent Approaches | This study aimed to analyze bacterial communities in breast milk obtained from five breastfeeding women. Culture-dependent and culture-independent methods were used to analyze microbial communities. Total bacterial count of breast milk determined using plate count agar ranged from 3.3 x 104 +/- 3.5 x 102 colony forming unit (CFU)/g to 1.7 x 105 +/- 3.5 x 103 CFU/g, with a pH between 6.4 and 6.8. Only three species, Leuconostoc citreum (17 out of 160 strains; 10.63%), Staphylococcus epidermidis (118 strains; 73.75%), and Staphylococcus lugdunensis (25 strains; 15.63%), belong to the phylum Bacillota were detected by culture-dependent analysis. Microbial communities analyzed via pyrosequencing revealed greater diversity compared to the culture-dependent analysis. At the phylum level, Bacillota accounted for 60.9% of the microbial community. At the genus level, Staphylococcus (24.57%), Streptococcus (22.93%), and Methylobacterium (8.76%) were dominant genera. While pyrosequencing demonstrated greater microbial diversity than the agar plate culture method, identified microbes might lack information or include many unculturable microbes. Most of all, considering the low total bacterial count averaging 7.2 x 104 CFU/g, further research is needed to determine the significance of microbial presence in breast milk. | Lee, Sumin; Heo, Sojeong; Park, Mi-Kyung; Sung, Moon-Hee; Jeong, Do-Won | Dongduk Womens Univ, Dept Food & Nutr, Seoul 02748, South Korea; Kyungpook Natl Univ, Sch Food Sci & Biotechnol, Daegu 41566, South Korea; Kyungpook Natl Univ, Bioind Res Inst, Daegu 41566, South Korea; KookminBio Corp, Seoul 02826, South Korea | Park, Mi-Kyung/J-9643-2017; LEE, SOOYEON/KAM-6204-2024 | 59391274100; 57191537574; 7404491155; 7202291188; 15623117800 | jeongdw@dongduk.ac.kr; | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | J MICROBIOL BIOTECHN | 1017-7825 | 1738-8872 | 34 | 10 | SCIE | MICROBIOLOGY;BIOTECHNOLOGY & APPLIED MICROBIOLOGY | 2024 | 3.1 | 44.4 | 0.19 | 2025-05-07 | 3 | 2 | Breast milk; bacterial community; culture-dependent; culture-independent | SP NOV. | bacterial community; Breast milk; culture-dependent; culture-independent | Adult; Bacteria; Bacterial Load; Biodiversity; Breast Feeding; DNA, Bacterial; Female; Humans; Microbiota; Milk, Human; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; agar; bacterial DNA; RNA 16S; adult; bacterial count; breast feeding; breast milk; colony forming unit; complication; drug therapy; female; human; Leuconostoc; Methylobacterium; microbial community; microbial diversity; middle aged; nonhuman; pH; pharmaceutics; phylum; plate count; pyrosequencing; review; Staphylococcus; Staphylococcus epidermidis; Staphylococcus lugdunensis; Streptococcus; bacterial load; bacterium; biodiversity; classification; DNA sequencing; genetics; isolation and purification; microbiology; microflora; phylogeny | English | 2024 | 2024-10 | 10.4014/jmb.2407.07001 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Biocontrol of Peach Gummosis by Bacillus velezensis KTA01 and Its Antifungal Mechanism | Peach tree gummosis is a botanical anomaly distinguished by the secretion of dark-brown gum from the shoots of peach trees, and Botryosphaeria dothidea has been identified as one of the fungal species responsible for its occurrence. In South Korea, approximately 80% of gummosis cases are linked to infections caused by B. dothidea. In this study, we isolated microbes from the soil surrounding peach trees exhibiting antifungal activity against B. dothidea. Subsequently, we identified several bacterial strains as potential candidates for a biocontrol agent. Among them, Bacillus velezensis KTA01 displayed the most robust antifungal activity and was therefore selected for further analysis. To investigate the antifungal mechanism of B. velezensis KTA01, we performed tests to assess cell wall degradation and siderophore production. Additionally, we conducted reverse transcriptionquantitative polymerase chain reaction (RT-qPCR) analysis based on whole-genome sequencing to confirm the presence of genes responsible for the biosynthesis of lipopeptide compounds, a wellknown characteristic of Bacillus spp., and to compare gene expression levels. Moreover, we extracted lipopeptide compounds using methanol and subjected them to both antifungal activity testing and high-performance liquid chromatography (HPLC) analysis. The experimental findings presented in this study unequivocally demonstrate the promising potential of B. velezensis KTA01 as a biocontrol agent against B. dothidea KACC45481, the pathogen responsible for causing peach tree gummosis. | Kang, Tae-An; Lee, Gyudae; Kim, Kihwan; Hahn, Dongyup; Shin, Jae-Ho; Kim, Won-Chan | Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea; Kyungpook Natl Univ, Dept Integrat Biol, Daegu 41566, South Korea; Kyungpook Natl Univ, NGS Core Facil, Daegu 41566, South Korea | 57652386200; 57222101785; 57210357476; 36554163400; 57224125922; 55492085000 | kwc@knu.ac.kr; | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | J MICROBIOL BIOTECHN | 1017-7825 | 1738-8872 | 34 | 2 | SCIE | MICROBIOLOGY;BIOTECHNOLOGY & APPLIED MICROBIOLOGY | 2024 | 3.1 | 44.4 | 0.93 | 2025-05-07 | 3 | 3 | Gummosis; biocontrol agent; Bacillus velezensis; Botryosphaeria dothidea; lipopeptide | BOTRYOSPHAERIA-DOTHIDEA; ANTIMICROBIAL LIPOPEPTIDE; DISEASE; TREES; IDENTIFICATION; SIDEROPHORES; RESISTANCE; PROVINCE | Bacillus velezensis; biocontrol agent; Botryosphaeria dothidea; Gummosis; lipopeptide | Antifungal Agents; Bacillus; Bacillus velezensis; Bacteria; Lipopeptides; cellulase; chitinase; DNA 16S; RNA 16S; antifungal agent; lipopeptide; antifungal activity; Article; Bacillus; Bacillus aerius; Bacillus amyloliquefaciens; Bacillus glycinifermentans; Bacillus subtilis; Bacillus velezensis; bacterial strain; bacterium isolate; biological pest control; Botryosphaeria; Botryosphaeria dothidea; cell wall; controlled study; DNA base composition; DNA sequence; enzyme activity; fungal plant disease; gene expression; gummosis; high performance liquid chromatography; nonhuman; peach; phylogenetic tree; reverse transcription polymerase chain reaction; RNA sequencing; whole genome sequencing; Bacillus; Bacillus velezensis; bacterium; chemistry; genetics; metabolism | English | 2024 | 2024-02 | 10.4014/jmb.2310.10005 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Review | Cellular Stress Responses against Coronavirus Infection: A Means of the Innate Antiviral Defense | Cellular stress responses are crucial for maintaining cellular homeostasis. Stress granules (SGs), activated by eIF2 alpha kinases in response to various stimuli, play a pivotal role in dealing with diverse stress conditions. Viral infection, as one kind of cellular stress, triggers specific cellular programs aimed at overcoming virus -induced stresses. Recent studies have revealed that virus -derived stress responses are tightly linked to the host's antiviral innate immunity. Virus infection -induced SGs act as platforms for antiviral sensors, facilitating the initiation of protective antiviral responses called "antiviral stress granules" (avSGs). However, many viruses, including coronaviruses, have evolved strategies to suppress avSG formation, thereby counteracting the host's immune responses. This review discusses the intricate relationship between cellular stress responses and antiviral innate immunity, with a specific focus on coronaviruses. Furthermore, the diverse mechanisms employed by viruses to counteract avSGs are described. | Yoo, Ji-Seung | Kyungpook Natl Univ, Sch Life Sci, FOUR KNU Creat BioRes Grp BK21, Daegu 41566, South Korea | Yoo, Ji-Seung/KHD-6177-2024 | 59510752300 | jisyoo@knu.ac.kr; | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | J MICROBIOL BIOTECHN | 1017-7825 | 1738-8872 | 34 | 1 | SCIE | MICROBIOLOGY;BIOTECHNOLOGY & APPLIED MICROBIOLOGY | 2024 | 3.1 | 44.4 | 0.96 | 2025-05-07 | 5 | 5 | Coronavirus; RIG-I-like receptors; toll-like receptors; antiviral stress granule; Interferon; virus immune evasion | DOUBLE-STRANDED-RNA; RIG-I; GRANULE FORMATION; HOST-DEFENSE; RECOGNITION; KINASE; TRANSLATION; INTERFERON; ACTIVATION; GCN2 | antiviral stress granule; Coronavirus; Interferon; RIG-I-like receptors; toll-like receptors; virus immune evasion | Antiviral Agents; Coronavirus; Coronavirus Infections; Humans; Immunity, Innate; Virus Diseases; dependent protein kinase; double stranded RNA; general control nonderepressible kinase 2; heme regulated inhibitor; initiation factor 2; PKR like ER kinase; retinoic acid receptor; toll like receptor; unclassified drug; antivirus agent; antiviral activity; cellular stress response; Coronavirus infection; enzyme activity; enzyme analysis; immune evasion; innate immunity; nonhuman; protein function; Review; stress granule; virus immunity; Coronavirinae; human; innate immunity; virus infection | English | 2024 | 2024-01 | 10.4014/jmb.2307.07038 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Change in the Gut Microbiota of Lactating Sows and Their Piglets by Inclusion of-Dried Plasma in Sow Diets | This study aimed to investigate the effects of dietary spray -dried plasma (SDP) on the gut microbiota of lactating sows and their piglets. A total of 12 sows were randomly assigned to one of two dietary treatment groups in a completely randomized design. The treatments were a sow diet based on corn and soybean meal (CON), and a CON diet with an added 1% SDP. The sows were fed the dietary treatments from d 30 before farrowing to weaning (d 28). The fecal samples of three sows from each treatment and two of their randomly selected piglets were collected to verify their fecal microbiota. There were no differences in the alpha diversity and distinct clustering of the microbial communities in the sows and their piglets when SDP was added to the sow diets from late gestation to weaning. The fecal microbiota of the lactating sows and their piglets showed a higher relative abundance of the phylum Bacteroidota and genus Lactobacillus and Ruminococcus and showed a lower relative abundance of the phylum Bacillota and genus Bacteroides, Escherichia/Shigella, and Clostridium in the sows fed the SDP diet than those fed the CON diet. Overall, these results show that the addition of SDP to the sow diet during lactation altered the gut environment with positive microbial composition changes. These results were similar in the nursing piglets, suggesting that the control of the sow diets during lactation may contribute to the intestinal health and growth in piglets after weaning. | Lee, Jeong Jae; Kyoung, Hyunjin; Cho, Jin Ho; Park, Kyeong Il; Kim, Yonghee; Ahn, Jinmu; Choe, Jeehwan; Kim, Younghoon; Kim, Hyeun Bum; Song, Minho | Kyungpook Natl Univ, Inst Agr Sci & Technol, Daegu 41566, South Korea; Chungnam Natl Univ, Div Anim & Dairy Sci, Daejeon 34134, South Korea; Chungbuk Natl Univ, Div Food & Anim Sci, Cheongju 28644, South Korea; Korea Natl Agr & Fisheries, Dept Floriculture, Jeonju 54874, South Korea; Seoul Natl Univ, Dept Agr Biotechnol, Seoul 08826, South Korea; Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul 08826, South Korea; Dankook Univ, Dept Anim Resources Sci, Cheonan 31116, South Korea | Kim, Hansu/F-5909-2013; Song, Minho/KHW-1844-2024 | 55915465100; 57213620137; 35228467000; 57749933600; 57709228800; 58967163000; 26631522600; 57861979600; 55784908300; 57200569587 | hbkim@dankook.ac.kr;mhsong@cnu.ac.kr; | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | J MICROBIOL BIOTECHN | 1017-7825 | 1738-8872 | 34 | 3 | SCIE | MICROBIOLOGY;BIOTECHNOLOGY & APPLIED MICROBIOLOGY | 2024 | 3.1 | 44.4 | 0.51 | 2025-04-16 | 2 | 2 | Dietary spray-dried plasma; fecal microbiota; lactating sows; nursing piglets | PERFORMANCE; PIGS; PCR | Dietary spray-dried plasma; fecal microbiota; lactating sows; nursing piglets | Animal Feed; Animals; Diet; Dietary Supplements; Feces; Female; Gastrointestinal Microbiome; Lactation; Pregnancy; Swine; Weaning; aerosol; animal experiment; article; Bacteroides; Clostridium; controlled study; diet; diet therapy; Escherichia; feces microflora; female; intestine; intestine flora; lactation; Lactobacillus; maize; microbial community; nonhuman; phylum; piglet; plasma; pregnancy; randomized controlled trial; Ruminococcus; Shigella; sow (swine); soybean meal; weaning; animal; animal food; diet; dietary supplement; feces; lactation; pig; veterinary medicine | English | 2024 | 2024-03 | 10.4014/jmb.2311.11001 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Effects of Microplastic Exposure against White Spot Syndrome Virus Infection in Pacific White Shrimp (Penaeus vannamei) | Plastic waste has emerged as a major environmental concern in recent years. As plastic waste discharged into the marine environment, it undergoes a breakdown process, eventually accumulating in aquatic organisms in the form of microplastics (MPs). To date, reduced food intake, nutritional absorption, and impaired immune system are known adverse effects of MPs-exposed aquatic organisms. This study aims to investigate whether MP exposure accelerated white spot syndrome virus (WSSV) infection in Pacific white shrimp (Penaeus vannamei) via laboratory tests. Briefly, experimental shrimp were divided into four groups; WSSV (group 1); MP (group 2); WSSV + MP (group 3); and Control (group 4). No mortality was observed in group 2, group 4, and even in group 1. However, group 3 showed a cumulative mortality of 50% during the experimental period. The PCR assay results showed no WSSV in the other three groups (groups 1, 2, and 4), but the dead and alive shrimp collected from group 3 were confirmed to be infected with the virus. Histopathological examination revealed normal structures in the hepatopancreas, gill, and muscle tissues of group 4, whereas numerous abnormally shaped nuclei were detected in the gill tissue of group 2. Moreover, group 1 showed minor WSSV-related lesions with few basophilic inclusion bodies in the gills, interestingly, group 3 exhibited severe lesions with numerous basophilic inclusion bodies in the gills. In conclusion, this study confirmed the correlation between the viral disease of shrimp and MPs, which can cause significant economic losses to the shrimp aquaculture industry. | Jeon, Hye Jin; Seo, Sangsu; Lee, Chorong; Kim, Bumkeun; Piamsomboon, Patharapol; Kim, Ji Hyung; Han, Jee Eun | Kyungpook Natl Univ, Coll Vet Med, Lab Aquat Biomed, Daegu 41566, South Korea; Chulalongkorn Univ, Fac Vet Sci, Dept Vet Med, Bangkok, Thailand; Chulalongkorn Univ, Vet Med Aquat Anim Res Ctr Excellence, Bangkok, Thailand; Gachon Univ, Dept Food Sci & Biotechnol, Seongnam 13120, South Korea; Kyungpook Natl Univ, Inst Vet Biomed Sci, Daegu 41566, South Korea | Kim, Ji Hyung/B-7321-2011 | 57212193058; 59150066600; 56707749200; 57225056167; 56106906400; 57225000204; 57214671240 | kzh81@gacheon.ac.kr;jehan@knu.ac.kr; | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | J MICROBIOL BIOTECHN | 1017-7825 | 1738-8872 | 34 | 8 | SCIE | MICROBIOLOGY;BIOTECHNOLOGY & APPLIED MICROBIOLOGY | 2024 | 3.1 | 44.4 | 0 | 2025-05-07 | 0 | 0 | Aquaculture; histopathology; microplastics; marine pollution; shrimp | WSSV; PCR | Aquaculture; histopathology; marine pollution; microplastics; shrimp | Animals; Aquaculture; Gills; Hepatopancreas; Microplastics; Penaeidae; Water Pollutants, Chemical; White spot syndrome virus 1; microplastic; microplastic; absorption; animal experiment; animal tissue; aquaculture; cell inclusion; controlled study; exposure; food intake; gill; hepatopancreas; histopathology; laboratory test; marine environment; mortality; muscle tissue; nonhuman; Penaeus vannamei; plastic waste; polymerase chain reaction; review; sea pollution; shrimp; virus infection; White spot syndrome virus; animal; aquaculture; gill; pathology; Penaeidae; toxicity; virology; water pollutant | English | 2024 | 2024-08 | 10.4014/jmb.2402.02001 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Effects of Peptidase Treatment on Properties of Yeast Protein as an Alternative Protein Source | Yeast protein, high-quality and high-content microbial protein, can serve as alternative sources of protein. This study examined the structural and functional characteristics of yeast protein through enzymatic treatment using different ratios of alcalase (endo-type) and prozyme 2000P (exo-type) including 2:1 (A2P1), 1:1 (A1P1), and 1:2 (A1P2). After enzymatic hydrolysis, a significant increase in protein solubility from less than 3.1% in untreated proteins to around 16%, particularly at pH 2 or pH 12. Furthermore, a maximum degree of hydrolysis of over 85% was achieved after enzyme treatment. Among them, the highest value of 87.73% was achieved at yeast protein treated by A1P2. Scanning electron microscopy images revealed varied surface morphologies, with exhibiting an increased surface area, particularly after treatment using A2P1. Next, yeast protein treated with A2P1 also demonstrated a superior emulsion stability index (3364.17). However, the antioxidant capacity was higher in proteins treated with A1P2 (78.30%). In addition, the elevated levels of certain amino acids, specifically leucine, lysine, phenylalanine, valine, and arginine, thereby indicating an enhanced amino acid profile was observed. Overall, yeast proteins treated with complex enzymes exhibited improved functionality and potential for diverse food applications. | Min, Ju Hyun; Istianah, Nur; Jang, Jeong Hwa; Jeon, Hyeon Ji; Jung, Young Hoon | Kyungpook Natl Univ, Sch Food Sci & Biotechnol, Daegu 41566, South Korea; Brawijaya Univ, Dept Food Sci & Biotechnol, Malang 65145, Indonesia | Jung, YoungHoon/LCE-3750-2024; Jeon, Hyeon Ji/HPH-4892-2023 | 57217835247; 56664484400; 57208244041; 57795638700; 55550063700 | younghoonjung@knu.ac.kr; | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | J MICROBIOL BIOTECHN | 1017-7825 | 1738-8872 | 34 | 12 | SCIE | MICROBIOLOGY;BIOTECHNOLOGY & APPLIED MICROBIOLOGY | 2024 | 3.1 | 44.4 | 0 | 2025-05-07 | 0 | 0 | Yeast protein hydrolysates; endotype protease; exotype protease; hydrolysis; alternative protein | FUNCTIONAL-PROPERTIES; HYDROLYSATE; RESOURCE; ALCALASE | alternative protein; endotype protease; exotype protease; hydrolysis; Yeast protein hydrolysates | Amino Acids; Antioxidants; Fungal Proteins; Hydrogen-Ion Concentration; Hydrolysis; Peptide Hydrolases; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Solubility; arginine; fungal protein; leucine; lysine; peptidase; phenylalanine; protein hydrolysate; subtilisin; unclassified drug; valine; yeast protein; amino acid; antioxidant; fungal protein; peptide hydrolase; Saccharomyces cerevisiae protein; ABTS radical scavenging assay; Article; Bacillus licheniformis; controlled study; enzymatic hydrolysis; Fourier transform infrared spectroscopy; molecular weight; morphology; nonhuman; particle size; pH; plant; polyacrylamide gel electrophoresis; protein degradation; protein function; protein structure; scanning electron microscopy; size exclusion chromatography; solubility; ultraviolet spectroscopy; water holding capacity; chemistry; hydrolysis; metabolism; Saccharomyces cerevisiae; solubility | English | 2024 | 2024-12 | 10.4014/jmb.2409.09062 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Enzymatic Activity and Amino Acids Production of Predominant Fungi from Traditional Meju during Soybean Fermentation | To investigate the effect of the predominant fungal species from Korean traditional meju and doenjang on soybean fermentation, the enzymatic activity and amino acid production of twentytwo fungal strains were assessed through solid- and liquid -state soybean fermentation. Enzymatic activity analyses of solid-state fermented soybeans revealed different enzyme activities involving protease, leucine aminopeptidase (LAP), carboxypeptidase (CaP), glutaminase, gamma-glutamyl transferase (GGT), and amylase, depending on the fungal species. These enzymatic activities significantly affected the amino acid profile throughout liquid -state fermentation. Strains belonging to Mucoromycota, including Lichtheimia, Mucor, Rhizomucor, and Rhizopus, produced smaller amounts of total amino acids and umami-producing amino acids, such as glutamic acid and aspartic acid, than strains belonging to Aspergillus subgenus circumdati. The genera Penicillium and Scopulariopsis produced large amounts of total amino acids and glutamic acid, suggesting that these genera play an essential role in producing umami and kokumi tastes in fermented soybean products. Strains belonging to Aspergillus subgenus circumdati, including A. oryzae, showed the highest amino acid content, including glutamic acid, suggesting the potential benefits of A. oryzae as a starter for soybean fermentation. This study showed the potential of traditional meju strains as starters for soybean fermentation. However, further analysis of processes such as the production of G -peptide for kokumi taste and volatile compounds for flavor and safety is needed. | Kim, Dong Hyun; Chun, Byung Hee; Lee, Jae-Jung; Kim, Oh Cheol; Hyun, Jiye; Han, Dong Min; Jeon, Che Ok; Lee, Sang Hun; Lee, Sang-Han; Choi, Yong-Ho; Hong, Seung-Beom | Natl Inst Agr Sci, Agr Microbiol Div, Korean Agr Culture Collect, Wonju 55365, South Korea; Pukyong Natl Univ, Dept Microbiol, Busan 48513, South Korea; Sempio Foods Co, Fermentat Res Lab, Fermentat R&D Ctr, Cheongju 28156, South Korea; Chung Ang Univ, Dept Life Sci, Seoul 06974, South Korea; Natl Inst Agr Sci, Food & Nutr Div, Wonju 55365, South Korea; Kyungpook Natl Univ, Dept Food Sci & Biotechnol, Daegu 41566, South Korea | KIM, SEONG/E-4164-2012; Lee, Seung Eun/ABG-1607-2021 | 57994755300; 57002535100; 56512578900; 58967835200; 57202446328; 57208507235; 57226512494; 57847972400; 57221453703; 56512745900; 35198177100 | funguy@korea.kr;cyongho@sempio.com; | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | J MICROBIOL BIOTECHN | 1017-7825 | 1738-8872 | 34 | 3 | SCIE | MICROBIOLOGY;BIOTECHNOLOGY & APPLIED MICROBIOLOGY | 2024 | 3.1 | 44.4 | 2.06 | 2025-04-16 | 6 | 7 | Meju soybean fermentation; fungi; enzyme activity; amino acid | SOY-SAUCE; STARTING MATERIAL; DOENJANG | amino acid; enzyme activity; fungi; Meju soybean fermentation | Amino Acids; Aspergillus; Fermentation; Fungi; Glutamic Acid; Glycine max; Peptide Hydrolases; Soy Foods; amino acid; amylase; aspartic acid; carboxypeptidase; cytosol aminopeptidase; gamma glutamyltransferase; glutamic acid; glutaminase; proteinase; volatile agent; glutamic acid; peptide hydrolase; Article; Aspergillus; Aspergillus oryzae; biosafety; circumdati; controlled study; enzyme activity; fermentation technique; flavor; fungal strain; fungus; Lichtheimia; liquid; meju; Mucor; nonhuman; Rhizomucor; Rhizopus; solid state; soybean; taste; traditional medicine; fermentation; metabolism; microbiology; soy food | English | 2024 | 2024-03 | 10.4014/jmb.2309.09008 | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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