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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | ○ | Article | GRP78 plays a key role in sperm function via the PI3K/PDK1/AKT pathway | Glucose-regulated protein 78 (GRP78), which is commonly found in the endoplasmic reticulum (ER), is involved in stabilizing ER proteins and inducing the unfolded protein response. Furthermore, GRP78 is expressed on the surface of most common cancer cells, such as cells of breast, lung, liver, and prostate cancers, and plays a role in apoptosis and cell proliferation via the PI3K/PDK1/AKT signaling pathway. Therefore, various trials have been performed for evaluating cancer treatment by inhibiting GRP78. Moreover, GRP78 is expressed on the surface of spermatozoa; however, its role in spermatozoa physiology remains unclear. Therefore, this study was designed to investigate the effects of GRP78 on sperm function during capacitation and elucidate the underlying mecha-nisms. Boar spermatozoa were exposed to various concentrations of HA15, a GRP78 antagonist, and sperm ki-nematic parameters, capacitation status, cell viability, levels of PI3K/PDK1/AKT-pathway related proteins, and tyrosine phosphorylation were evaluated. GRP78 inhibition significantly decreased sperm motility, kinematic parameters, capacitated and acrosome-reacted spermatozoa counts, and cell viability. Moreover, GRP78 expression was significantly decreased in HA15-treated spermatozoa compared to that in the control group, and levels of PI3K/PDK1/AKT-pathway related proteins changed significantly. Furthermore, tyrosine phosphoryla-tion was significantly altered in the HA15-treated group. The results of this study suggest that GRP78 inhibition in cancer therapy may negatively affect sperm function. These results lay a strong foundation for future studies aiming to identify the molecular mechanisms related to GRP78 in spermatozoa. | Lee, Woo-Jin; Jung, Eun-Ju; Hwang, Ju-Mi; Bae, Jeong-Won; Kwon, Woo-Sung | Kyungpook Natl Univ, Dept Anim Sci & Biotechnol, Sangju 37224, Gyeongsangbugdo, South Korea | Bae, Jeong-Won/AAH-4932-2021; Kwon, Woo-Sung/J-6731-2019 | 57377138800; 57377289200; 57217871526; 57211231093; 54383715800 | wskwon@knu.ac.kr; | REPRODUCTIVE TOXICOLOGY | REPROD TOXICOL | 0890-6238 | 1873-1708 | 113 | SCIE | REPRODUCTIVE BIOLOGY;TOXICOLOGY | 2022 | 3.3 | 37.8 | 1.38 | 2025-06-25 | 14 | 14 | GRP78; Sperm; PI3K; PDK1; AKT pathway; Capacitation; GRP78; Sperm; PI3K; PDK1; AKT pathway; Capacitation | REGULATOR GRP78/BIP; SIGNALING PATHWAY; SURFACE; CELLS; CHAPERONE; PROTEINS; RECEPTOR; CANCER; HSP60; LOCALIZATION | Capacitation; GRP78; PI3K/PDK1/AKT pathway; Sperm | Animals; Male; Phosphatidylinositol 3-Kinases; Phosphorylation; Proto-Oncogene Proteins c-akt; Semen; Sperm Capacitation; Sperm Motility; Spermatozoa; Swine; Tyrosine; endoplasmic reticulum chaperone BiP; hemagglutinin; phosphoinositide dependent protein kinase 1; protein tyrosine kinase; phosphatidylinositol 3 kinase; protein kinase B; tyrosine; acrosome; Article; cancer therapy; cell viability; controlled study; enzyme phosphorylation; human; human cell; kinematics; male; Pi3K/Akt signaling; protein expression; protein function; semen parameters; sperm capacitation; sperm function; spermatozoon; spermatozoon motility; animal; metabolism; phosphorylation; pig; sperm; sperm capacitation; spermatozoon motility | English | 2022 | 2022-10 | 10.1016/j.reprotox.2022.08.008 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Isolated hypoaldosteronism managed by DOCP in a dog with chronic kidney disease and hypercortisolism | A 13-year-old spayed female Schnauzer dog with chronic kidney disease (CKD; International Renal Interest Society stage 2, non-proteinuric, normotensive), diabetes mellitus, hypercortisolism and myxomatous mitral valve degeneration (American College of Veterinary Internal Medicine stage B2) presented with electrolyte imbalance that had progressed to hyperkalaemia and hyponatremia, with a sodium to potassium (Na:K) ratio of 19.6. Cortisol levels after the adrenocorticotropic hormone stimulation test were within the therapeutic range, but aldosterone levels were below the reference range; hence, isolated hypoaldosteronism was diagnosed. After administration of deoxycorticosterone pivalate (DOCP), the electrolyte imbalance improved with a Na:K ratio of 27.7. This is the first report of the management of isolated hypoaldosteronism and hypercortisolism using trilostane and DOCP in a dog. This case highlights the importance of recognizing isolated hypoaldosteronism after long-term treatment with trilostane in a canine patient with CKD. | Park, Su-Min; An, Ju-Hyun; Kim, Na-Hyeong; Oh, Ye-In; Seo, Kyoung-won; Youn, Hwa-Young | Seoul Natl Univ, Dept Vet Clin Sci, Lab Vet Internal Med, Coll Vet Med, Seoul 08826, South Korea; Seoul Natl Univ, Res Inst Vet Sci, Seoul, South Korea; Kangwon Natl Univ, Coll Vet Med, Dept Vet Emergency & Crit Care Med, Chuncheon Si, South Korea; Kangwon Natl Univ, Coll Vet Med, Inst Vet Sci, Chuncheon Si, South Korea; Kyungpook Natl Univ, Dept Vet Internal Med, Coll Vet Med, Daegu, South Korea | Seo, Kwang/AAB-4821-2020 | 57211909684; 57206839650; 57888244200; 36466903200; 8833507700; 7102027331 | hyyoun@snu.ac.kr; | VETERINARY MEDICINE AND SCIENCE | VET MED SCI | 2053-1095 | 8 | 6 | SCIE | VETERINARY SCIENCES | 2022 | 1.7 | 37.8 | 0.22 | 2025-06-25 | 1 | 1 | chronic kidney disease; deoxycorticosterone pivalate; dog; hypoaldosteronism; trilostane | TERM-FOLLOW-UP; HYPORENINEMIC HYPOALDOSTERONISM; DEOXYCORTICOSTERONE PIVALATE; HYPERKALEMIA; TRILOSTANE; HYPOADRENOCORTICISM; HYPERADRENOCORTICISM; INHIBITORS | chronic kidney disease; deoxycorticosterone pivalate; dog; hypoaldosteronism; trilostane | Animals; Cushing Syndrome; Dog Diseases; Dogs; Electrolytes; Female; Hypoaldosteronism; Potassium; Renal Insufficiency, Chronic; Sodium; 6 n,n' dimethylarginine; aldosterone; corticotropin; creatinine; deoxycorticosterone pivalate; hydrocortisone; isophane insulin; pimobendan; potassium; sodium; trilostane; electrolyte; potassium; Article; blood pressure; chronic kidney failure; diabetes mellitus; dog; electrolyte disturbance; female; hypercortisolism; hyperkalemia; hypoaldosteronism; hyponatremia; mitral valve; nonhuman; proteinuria; animal; case report; complication; Cushing syndrome; dog; dog disease; hypoaldosteronism; veterinary medicine | English | 2022 | 2022-11 | 10.1002/vms3.954 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Isopanepoxydone inhibits oxidative damage in murine alveolar macrophages via NRF2 and NLRP3 inflammasome | Background: Respiratory diseases due to particulate matter are a serious health issue. We sought to investigate the efficacy of isopanepoxydone (ISO) isolated from the Panus rudis as a therapeutic against particulate matter-induced respiratory complications. Materials and Methods: ISO was isolated from a culture broth of Panus rudis using solvent partition, silica gel, and column chromatography, and high-performance liquid chromatography. Its chemical structure was determined spectroscopically. Murine alveolar macrophages (MH-S) were treated with ISO to investigate the inhibition of nitric oxide (NO) while cytotoxicity was investigated via a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. The expression of pro-inflammatory mediators, cytokines, and protein expression levels in the oxidative protective and inflammasome pathway were also investigated. Reactive oxygen species in MH-S cells were investigated using 2',7'-dichlorofluorescein diacetate while immunofluorescence was performed to investigate the expression of activated apoptosis-associated speck-like proteins (ASC) containing a caspase recruitment domain in MH-S cells. Results: ISO effectively inhibited CFA-induced NO production with no cytotoxicity on MH-S cells and pro-inflammatory mediators and cytokines were also inhibited (except tumor necrosis factor a and interleukin-6). ISO enhanced the protein expression of nuclear factor erythroid 2-related factor 2, while suppressing proteins in the inflammasome pathway, but did not suppress the expression of nuclear factor-kappa B. ISO also reduced detectable ROS other than preventing the activation of ASC. Conclusion: Pathways of action of ISO in MH-S cells that prevent oxidative damage and suppress the expression of proteins in the inflammasome pathway were investigated. ISO may be developed as a treatment for respiratory inflammation. | Lee, Yuan Yee; Ullah, H. M. Arif; Ha, Lee Su; Kim, Sung Dae; Yun, Bong-Sik; Rhee, Man Hee | Kyungpook Natl Univ, Dept Vet Med, Daegu, South Korea; Jeonbuk Natl Univ, Coll Environm & Bioresource Sci, Div Biotechnol & Adv Inst Environm & Biosci, Jeonbuk, South Korea | ; Yuan Yee, Lee/ABH-8956-2022; Ullah, H/AAE-5513-2021; Rhee, Man/O-5705-2016 | 57203798815; 57198885380; 57208421087; 55156746000; 7201805685; 57211035357 | bsyun@jbnu.ac.kr;rheemh@knu.ac.kr; | IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY | IMMUNOPHARM IMMUNOT | 0892-3973 | 1532-2513 | 44 | 3 | SCIE | IMMUNOLOGY;PHARMACOLOGY & PHARMACY;TOXICOLOGY | 2022 | 3.3 | 37.8 | 0.09 | 2025-06-25 | 1 | 1 | Isopanepoxydone; reactive oxygen species; NF-kappa B; lung inflammation; MH-S | PARTICULATE MATTER; PATHOPHYSIOLOGY; STRIGELLUS; METABOLITE; EXPOSURE; DISEASE; RISK | Isopanepoxydone; lung inflammation; MH-S; NF-κB; reactive oxygen species | Animals; Cytokines; Inflammasomes; Inflammation Mediators; Macrophages, Alveolar; Mice; NF-E2-Related Factor 2; NLR Family, Pyrin Domain-Containing 3 Protein; Oxidative Stress; Particulate Matter; Polyporales; autacoid; cryopyrin; cytokine; inflammasome; Nlrp3 protein, mouse; transcription factor Nrf2; animal; lung alveolus macrophage; metabolism; mouse; oxidative stress; particulate matter; Polyporales | English | 2022 | 2022-05-04 | 10.1080/08923973.2022.2047197 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Nasopharyngeal collapse can be identified on radiography in healthy male Beagle dogs without cardiopulmonary diseases | Pharyngeal collapse has been described as a decrease of more than 50% in the diameter of the lumen. However, there has been no study on changes in the pharyngeal lumen in clinically normal dogs. The hypothesis of this prospective, observational, and pilot study was that change in nasopharyngeal lumen would be over 50% in Beagle dogs without cardiopulmonary diseases. Thus, we assessed the nasopharyngeal luminal change using cervical radiography in Beagle dogs without respiratory signs or cardiac abnormalities. The study sample included 42 Beagle dogs without cardiopulmonary diseases. Cervical radiographies were acquired during inspiration and expiration, and nasopharyngeal luminal change (%) was calculated. The median nasopharyngeal luminal change was 16.6%, and nasopharyngeal luminal change over 50% was identified in two dogs. The nasopharyngeal luminal change was significantly associated with body condition score and body weight. There was no correlation between nasopharyngeal luminal change and age. This study revealed that change in nasopharyngeal lumen over 50% can be identified in Beagle dogs without cardiopulmonary diseases and may be over-diagnosed as partial pharyngeal collapse. Further studies for comparing change in nasopharyngeal lumen between clinically normal dogs and dogs with respiratory signs are warranted. | Na, Hyemin; Lee, Sang-Kwon; Choi, Hojung; Lee, Youngwon; Lee, Kija | Kyungpook Natl Univ, Coll Vet Med, 80 Daehak Ro, Daegu 41566, South Korea; Chungnam Natl Univ, Coll Vet Med, Daejeon 34134, South Korea | 57219434344; 56494541400; 35202930200; 8983566800; 35311016000 | leekj@knu.ac.kr; | VETERINARY RADIOLOGY & ULTRASOUND | VET RADIOL ULTRASOUN | 1058-8183 | 1740-8261 | 63 | 5 | SCIE | VETERINARY SCIENCES | 2022 | 1.7 | 37.8 | 0.43 | 2025-06-25 | 2 | 2 | airway collapse; canine; cervical radiography | OBSTRUCTIVE SLEEP-APNEA; COMPUTED-TOMOGRAPHY; PHARYNGEAL COLLAPSE; HEAD POSTURE; UPPER AIRWAY; OBESITY | airway collapse; canine; cervical radiography | Animals; Dog Diseases; Dogs; Male; Pharyngeal Diseases; Pharynx; Pilot Projects; Prospective Studies; Radiography; animal experiment; Article; beagle; body constitution; body weight; bone density; breathing pattern; cervical spine radiography; endotracheal intubation; expired air; lung auscultation; lung parenchyma; male; nasopharyngeal collapse; nonhuman; observational study; pharynx disease; pilot study; radiography; sneezing; vertebral canal; animal; diagnostic imaging; dog; dog disease; pharynx; prospective study; radiography; veterinary medicine | English | 2022 | 2022-09 | 10.1111/vru.13094 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Reproductive toxicity of ritonavir in male: Insight into mouse sperm capacitation | Since COVID-19 began in 2019, therapeutic agents are being developed for its treatment. Among the numerous potential therapeutic agents, ritonavir (RTV), an anti-viral agent, has recently been identified as an important element of the COVID-19 treatment. Moreover, RTV has also been applied in the drug repurposing of cancer cells. However, previous studies have shown that RTV has toxic effects on various cell types. In addition, RTV regulates AKT phosphorylation within cancer cells, and AKT is known to control sperm functions (motility, capacitation, and so on). Although deleterious effects of RTV have been reported, it is not known whether RTV has male reproduction toxicity. Therefore, in this study, we aimed to investigate the effects of RTV on sperm function and male fertility. In the present study, sperm collected from the cauda epididymis of mice were incubated with various concentrations of RTV (0, 0.1, 1, 10, and 100 mu M). The expression levels of AKT, phospho-AKT (Thr308 and Ser473), and phospho-tyrosine proteins, sperm motility, motion kinematics, capacitation status, and cell viability were assessed after capacitation. The results revealed that AKT phosphorylation at Thr308 and Ser473 was significantly increased, and the levels of tyrosine-phosphorylated proteins (at approximately 25 and 100 kDa) were significantly increased in a dose-dependent manner. In addition, RTV adversely affected sperm motility, motion kinematics, and cell viability. Taken together, RTV may have negative effects on sperm function through an abnormal increase in tyrosine phosphorylation and phospho-AKT levels. Therefore, individuals taking or prescribing RTV should be aware of its reproductive toxicity. | Jung, Eun-Ju; Lee, Woo-Jin; Hwang, Ju-Mi; Bae, Jeong-Won; Kwon, Woo-Sung | Kyungpook Natl Univ, Dept Anim Sci & Biotechnol, Sangju 37224, Gyeongsangbuk D, South Korea | Bae, Jeong-Won/AAH-4932-2021; Kwon, Woo-Sung/J-6731-2019 | 57377289200; 57377138800; 57217871526; 57211231093; 54383715800 | wskwon@knu.ac.kr; | REPRODUCTIVE TOXICOLOGY | REPROD TOXICOL | 0890-6238 | 1873-1708 | 114 | SCIE | REPRODUCTIVE BIOLOGY;TOXICOLOGY | 2022 | 3.3 | 37.8 | 0.99 | 2025-06-25 | 10 | 10 | Ritonavir; Spermatozoa; Capacitation; AKT pathway; Male fertility | PROTEASE INHIBITOR RITONAVIR; MOTILITY; PATHWAY; APOPTOSIS; AKT/PKB | AKT pathway; Capacitation; Male fertility; Ritonavir; Spermatozoa | Animals; COVID-19; Humans; Male; Mice; Phosphorylation; Proto-Oncogene Proteins c-akt; Ritonavir; Semen; Sperm Capacitation; Sperm Motility; Spermatozoa; Tyrosine; alpha tubulin; chlortetracycline; immunoglobulin G; phosphotyrosine; povidone; protein kinase B; ritonavir; serine; thyroxine; protein kinase B; ritonavir; tyrosine; acrosome reaction; adult; amplitude of lateral head displacement; animal cell; animal experiment; animal model; animal tissue; Article; average path velocity; beat cross frequency; cauda epididymis; cell motility; cell viability; centrifugation; chemiluminescence immunoassay; comparative study; computer assisted sperm analysis program; controlled study; coronavirus disease 2019; curvilinear velocity; drug repositioning; fluorescence; incubation time; kinematics; linearity; male; male fertility; mean angular displacement; medium sperm motility; microtubule; motion kinematics; mouse; nonhuman; polyacrylamide gel electrophoresis; progressive sperm motility; protein expression; protein phosphorylation; rapid sperm motility; reproductive toxicity; semen analysis; slow sperm motility; sperm activation; sperm capacitation; sperm function; sperm preservation; spermatozoon; spermatozoon density; spermatozoon motility; straight line velocity; straightness; Western blotting; wobble; animal; human; metabolism; phosphorylation; sperm; spermatozoon | English | 2022 | 2022-12 | 10.1016/j.reprotox.2022.09.008 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Spiritual leadership and employee innovation | This study examines the effects of spiritual leadership on employees' innovative work behavior (IWB) and how employees' spiritual well-being intervenes this relationship. The research was based on a self-reported survey of 264 workers in China and was analyzed through multiple regression in structural equation modeling to test the hypothesized relationships. The results of the study concluded that employees' IWB is positively influenced by the effects of spiritual leadership. Furthermore, the effects of spiritual well-being intervened in explaining how spiritual leadership influences employee innovation. This study suggests that the innovative behavior of employees can be enhanced through initiating spiritual leadership practices that enable a spiritual workplace, which, in turn, can help organizations to more effectively cope with competitive market pressures to continuously innovate. Moreover, the study expands our current understanding of the role employee well-being can play in enriching individual creativity and innovation. | Hunsaker, William D. | Kyungpook Natl Univ, Sch Business Adm, 80 Daehak Ro, Daegu 41566, South Korea | Hunsaker, William/E-9696-2017 | 55974245100 | hunsaker@knu.ac.kr; | CURRENT PSYCHOLOGY | CURR PSYCHOL | 1046-1310 | 1936-4733 | 41 | 8 | SSCI | PSYCHOLOGY, MULTIDISCIPLINARY | 2022 | 2.8 | 37.8 | 4.62 | 2025-06-25 | 37 | 40 | employee well-being; innovative work behavior; spiritual leadership; spiritual well-being; workplace spirituality | TRANSFORMATIONAL LEADERSHIP; WORKPLACE SPIRITUALITY; ORGANIZATIONAL CULTURE; INDIVIDUAL INNOVATION; MODERATING ROLE; WORK; BEHAVIOR; CREATIVITY; DETERMINANTS; PERFORMANCE | employee well-being; innovative work behavior; spiritual leadership; spiritual well-being; workplace spirituality | English | 2022 | 2022-08 | 10.1007/s12144-020-01011-9 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Modeling Crime Density with Population Dynamics in Space and Time: An Application of Assault in Gangnam, South Korea | The current study explores populational and environmental factors associated with violent crime. Specifically, it compares ambient and residential populations with regard to their association with assault density at a fine spatial and temporal unit in a city with socio-economic control variables. The results show that the ambient population are consistently associated with the level of assaults throughout the four time periods in a day, while residential population does not contribute much to explaining its variation. In addition, we also find that the percentage of single-member households and the distance to the nearest subway station are constantly associated with assault density, while the proportion of non-residential use and the land price are partially associated. | Jung, Yeondae; Chun, Yongwan; Kim, Kamyoung | Univ Texas Dallas, Geospatial Informat Sci, Richardson, TX 75080 USA; Kyungpook Natl Univ, Geog Educ, Daegu, South Korea | 57207947218; 24802081100; 16022369700 | yeondae.jung@utdallas.edu; | CRIME & DELINQUENCY | CRIME DELINQUENCY | 0011-1287 | 1552-387X | 68 | 2 | SSCI | CRIMINOLOGY & PENOLOGY | 2022 | 2.1 | 37.9 | 1.46 | 2025-06-25 | 4 | 7 | ambient population; space-time analysis; assault | SOCIAL-DISORGANIZATION; ROUTINE ACTIVITY; LAND-USE; AMBIENT POPULATION; SPATIAL-ANALYSIS; STREET ROBBERY; VIOLENT CRIME; BUS STOPS; OVERDISPERSION; DISADVANTAGE | ambient population; assault; space-time analysis | English | 2022 | 2022-02 | 10.1177/0011128720948037 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Determinants of left ventricular function improvement for cardiac resynchronization therapy candidates | Aims A waiting period of more than 3 months is recommended for patients before undergoing cardiac resynchronization therapy (CRT). However, due to an anticipated high mortality rate, early implementation of CRT might be beneficial for some patients. We aimed to evaluate the rate and the probability of left ventricular (LV) function improvement and their predictors in patients with heart failure (HF) with indications for CRT. Methods and results From March 2011 to February 2014, a total of 5625 hospitalized patients for acute HF were consecutively enrolled in 10 tertiary hospitals. Among them, we analysed 1792 patients (mean age 63.96 +/- 15.42 years, female 63.1%) with left ventricular ejection fraction (LVEF) <= 35% at the baseline echocardiography and divided them into three groups: 144 with left bundle branch block (LBBB), 136 with wide QRS complexes without LBBB, and 1512 not having these findings (control). We compared and analysed these three groups for improvement of LV function at follow-up echocardiography. In patients who met CRT indications (patients with LBBB or wide QRS complexes without LBBB), logistic regression was performed to identify risk factors for no improvement of LV. No improvement of LV was defined as LVEF <= 35% at follow-up echocardiography or the composite adverse outcomes: death, heart transplantation, extracorporeal membrane oxygenation, or use of a ventricular assist device before follow-up echocardiography. A classification tree was established using the binary recursive partitioning method to predict the outcome of patients who met CRT indications. In a median follow-up of 11 months, LVEF improvement was observed in 24.3%, 15.4%, and 40.5% of patients with LBBB, wide QRS complexes without LBBB, and control, respectively. Patients meeting CRT indications had higher 3 month mortality rates than the control (24.6% vs. 17.7%, P = 0.002). Multivariable logistic regression analysis revealed that large LV end-systolic dimension [odds ratio (OR) 1.10, 95% confidence interval (CI) 1.05-1.15, P < 0.001], low LVEF (OR 0.92, 95% CI 0.87-0.98, P = 0.006), diabetes requiring insulin (OR 6.49, 95% CI 2.53-19.33, P < 0.001), and suboptimal medical therapy (OR 6.85, 95% CI 3.21-15.87, P < 0.001) were significant factors predictive of no improvement. A decision tree analysis was consistent with these results. Conclusions Patients with CRT indications had higher mortality during their follow-up compared with control. LV function improvement was rare in this population, especially when they had some risk factors. These results suggest that the uniform waiting period before CRT implantation could be reconsidered and individualized. | Hong, Jung Ae; Lee, Sang Eun; Kim, Seon-Ok; Kim, Min-Seok; Lee, Hae-Young; Cho, Hyun-Jai; Choi, Jin Oh; Jeon, Eun-Seok; Hwang, Kyung-Kuk; Chae, Shung Chull; Baek, Sang Hong; Kang, Seok-Min; Choi, Dong-Ju; Yoo, Byung-Su; Kim, Kye Hun; Cho, Myeong-Chan; Oh, Byung-Hee; Kim, Jae-Joong | Univ Ulsan, Man Med Ctr, Dept Cardiol, Coll Med, 88 Olymp Ro 43 Gil, Seoul 05505, South Korea; Univ Ulsan, Man Med Ctr, Dept Cardiovasc Surg, Coll Med, Seoul, South Korea; Univ Ulsan, Man Med Ctr, Dept Clin Epidemiol & Blostat, Coll Med, Seoul, South Korea; Seoul Natl Univ Hosp, Dept Internal Med, Seoul, South Korea; Sungkyunkwan Univ, Coll Medidne, Seoul, South Korea; Chungbuk Natl Univ, Coll Med, Cheongju, South Korea; Kyungpook Natl Univ, Coll Med, Daegu, South Korea; Catholic Univ Korea, Seoul, South Korea; Yonsei Univ, Coll Med, Seoul, South Korea; Seoul Natl Univ, Bundang Hosp, Seongnam, South Korea; Yonsei Univ, Wonju Coll Med, Wonju, South Korea; Chonnam Natl Univ, Heart Res Ctr, Gwangju, South Korea; Mediplex Sejong Hosp, Cardiovasc Ctr, Div Cardiol, Incheon, South Korea | LEE, JI/L-6920-2013; KIM, SEON-OK/HGC-7012-2022; Oh, Byung-Hee/G-9875-2011; Choi, Dong-Ju/J-5686-2012; Kim, Soo-Yeon/ADR-9663-2022; Choi, Joon/D-6140-2017; Jeong, Gi/AAB-2830-2021 | 57195584507; 57207065107; 57196230629; 57212315719; 56151235500; 35285421400; 15848011800; 7004279641; 7402426370; 7101962036; 7201371594; 7405685375; 35274349200; 7102851884; 56150430800; 7401727518; 57216293873; 36065764100 | sangeunlee.md@gmail.com;sangeunlee.md@amc.seoul.kr; | ESC HEART FAILURE | ESC HEART FAIL | 2055-5822 | 9 | 1 | SCIE | CARDIAC & CARDIOVASCULAR SYSTEMS | 2022 | 3.8 | 38.1 | 0.23 | 2025-06-25 | 2 | 2 | Heart failure; Reduced ejection fraction; Cardiac resynchronization therapy; Waiting period | ACUTE HEART-FAILURE; REDUCED EJECTION FRACTION; AMERICAN-COLLEGE; TASK-FORCE; DEFIBRILLATOR; PREDICTORS; GUIDELINE; MORTALITY; ASSOCIATION; MEDICATIONS | Aged; Cardiac Resynchronization Therapy; Electrocardiography; Female; Humans; Middle Aged; Stroke Volume; Treatment Outcome; Ventricular Function, Left; insulin; adult; adverse outcome; Article; cardiac resynchronization therapy; diabetes mellitus; female; heart left bundle branch block; heart left ventricle ejection fraction; heart left ventricle function; human; major clinical study; male; mortality rate; probability; QRS complex; risk factor; treatment indication; aged; electrocardiography; heart left ventricle function; heart stroke volume; middle aged; procedures; treatment outcome | English | 2022 | 2022-02 | 10.1002/ehf2.13765 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Modified reverse shock index predicts early outcomes of heart failure with reduced ejection fraction | Aims Increased blood pressure (BP) and decreased heart rate (HR) are signs of stabilization in patients admitted for acute HF. Changes in BP and HR during admission and their correlation with outcomes were assessed in hospitalized patients with heart failure (HF) with reduced ejection fraction (HFrEF). Methods A novel modified reverse shock index (mRSI), defined as the ratio between changes in systolic BP and HR during admission, was devised, and its prognostic value in the early outcomes of acute HF was assessed using the Korean Acute HF registry. Results Among 2697 patients with HFrEF (mean age 65.8 +/- 14.9 years, 60.6% males), patients with mRSI >= 1.25 at discharge were significantly younger and were more likely to have de novo HF. An mRSI >= 1.25 was associated with a significantly lower incidence of 60-day and 180-day all-cause mortality [hazard ratio (HR) 0.49, 95% confidence interval (CI) 0.31-0.77; HR 0.62, 95% CI 0.45-0.85, respectively), compared with 1 1.25 was consistent in sub-group analyses. The correlation of mRSI and outcomes were also consistent regardless of admission SBP, presence of atrial fibrillation, or use of beta blockers at discharge. Conclusions In patients hospitalized for HFrEF, the mRSI was a significant predictor of early outcomes. The mRSI could be used as a tool to assess patient status and guide physicians in treating patients with HFrEF. | Oh, Gyu Chul; An, Seokyung; Lee, Hae-Young; Cho, Hyun-Jai; Jeon, Eun-Seok; Lee, Sang Eun; Kim, Jae-Joong; Kang, Seok-Min; Hwang, Kyung-Kuk; Cho, Myeong-Chan; Chae, Shung Chull; Choi, Dong-Ju; Yoo, Byung-Su; Kim, Kye Hun; Park, Sue K.; Baek, Sang Hong | Catholic Univ Korea, Coll Med, Dept Internal Med, Seoul, South Korea; Catholic Univ Korea, Coll Med, Catholic Res Inst Intractable Cardiovasc Dis CRID, Seoul, South Korea; Seoul Natl Univ, Grad Sch, Dept Biomed Sci, Seoul, South Korea; Seoul Natl Univ, Canc Res Inst, Seoul, South Korea; Seoul Natl Univ Hosp, Dept Internal Med, Seoul, South Korea; Sungkyunkwan Univ, Coll Med, Dept Internal Med, Seoul, South Korea; Univ Ulsan, Coll Med, Asan Med Ctr, Dept Internal Med, Seoul, South Korea; Yonsei Univ, Coll Med, Dept Internal Med, Seoul, South Korea; Chungbuk Natl Univ, Coll Med, Dept Internal Med, Cheongju, South Korea; Kyungpook Natl Univ, Sch Med, Dept Internal Med, Daegu, South Korea; Seoul Natl Univ, Bundang Hosp, Dept Internal Med, Seongnam, South Korea; Yonsei Univ, Wonju Coll Med, Dept Internal Med, Wonju, South Korea; Chonnam Natl Univ, Heart Res Ctr, Dept Internal Med, Gwangju, South Korea; Seoul Natl Univ, Grad Sch, Dept Integrated Major Innovat Med Sci, Seoul, South Korea | ; Oh, Gyu/AAJ-6530-2021; LEE, JI/L-6920-2013; An, Seokyung/AAT-1785-2020; choi, jo/O-5940-2014; Choi, Dong-Ju/J-5686-2012; Jeong, Gi/AAB-2830-2021 | 52264473100; 57210703966; 56151235500; 35285421400; 7004279641; 57207065107; 36065764100; 7405685375; 7402426370; 7401727518; 7101962036; 35274349200; 7102851884; 56150430800; 8085325400; 7201371594 | hylee612@snu.ac.kr; | ESC HEART FAILURE | ESC HEART FAIL | 2055-5822 | 9 | 5 | SCIE | CARDIAC & CARDIOVASCULAR SYSTEMS | 2022 | 3.8 | 38.1 | 0.47 | 2025-06-25 | 3 | 4 | Heart failure; Blood pressure; Heart rate; Mortality | BLOOD-PRESSURE; PROGNOSTIC VALUE; HOSPITALIZATION; GUIDELINES; DIAGNOSIS; ESC | Blood pressure; Heart failure; Heart rate; Mortality | Adrenergic beta-Antagonists; Aged; Aged, 80 and over; Female; Heart Failure; Hospitalization; Humans; Male; Middle Aged; Stroke Volume; Ventricular Dysfunction, Left; aldosterone antagonist; angiotensin receptor antagonist; beta adrenergic receptor blocking agent; beta adrenergic receptor blocking agent; adult; aged; all cause mortality; Article; atrial fibrillation; cardiovascular mortality; cohort analysis; confidence interval; controlled study; diastolic blood pressure; female; hazard ratio; heart failure with reduced ejection fraction; heart rate; hospital admission; hospital discharge; human; incidence; major clinical study; male; modified reverse shock index; outcome assessment; patient registry; prognosis; systolic blood pressure; heart failure; heart left ventricle function; heart stroke volume; hospitalization; middle aged; physiology; very elderly | English | 2022 | 2022-10 | 10.1002/ehf2.14031 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Real-world eligibility for vericiguat in decompensated heart failure with reduced ejection fraction | Aims In 2021, vericiguat was approved by the US Food and Drug Administration (FDA) and the European Commission (EC) for reducing cardiovascular mortality and heart failure (HF) hospitalizations in patients with HF with reduced ejection fraction (HFrEF) based on the Vericiguat Global Study in Subjects with Heart Failure with Reduced Ejection Fraction (VICTORIA) trial. However, there has been no report for characterizing the generalizability of vericiguat to real-world clinical practice. Methods and results The Korean Acute Heart Failure (KorAHF) registry is a multicentre prospective cohort study. A total of 5625 patients who were admitted for HF decompensation were consecutively enrolled. We excluded the patients without left ventricular ejection fraction (LVEF) quantification, patients with LVEF > 45%, patients with in-hospital death or urgent heart transplantation, and patients without natriuretic peptide measurement. Among a total of 3014 enrolled patients, there were 21.9% patients with lower systolic blood pressure (SBP) (<100 mmHg) and 20.1% patients without elevated natriuretic peptide. Regarding chronic kidney disease (CKD) status, 5.1% patients had CKD Stage V [estimated glomerular filtration rate (eGFR) <15 mL/min/1.73 m(2)] and 11.8% patients had CKD Stage IV (15 <= eGFR < 30 mL/min/1.73 m(2)). When we analysed these criteria sequentially, 21.9% were excluded from lower SBP, 15.9% were excluded from elevated natriuretic peptide, and 4.2% were excluded from advanced CKD Stage V (9.6% for CKD Stages IV and V). Among the KorAHF registry patients, we found two main reasons for not meeting the inclusion criteria of the VICTORIA trial such as low SBP and non-elevated natriuretic peptide. Conclusions Among the Korean hospitalized HFrEF patients, 94.9% met the FDA/EC label criteria, while 58% met the inclusion criteria of the VICTORIA trial. Our findings suggest the need for better strategies to integrate up-to-date HF treatment in a real-world HF population, especially decompensated HF patients with low SBP and non-elevated natriuretic peptide. | Oh, Jaewon; Lee, Chan Joo; Park, Jin Joo; Lee, Sang Eun; Kim, Min-Seok; Cho, Hyun-Jai; Choi, Jin-Oh; Lee, Hae-Young; Hwang, Kyung-Kuk; Kim, Kye Hun; Yoo, Byung-Su; Choi, Dong-Ju; Baek, Sang Hong; Jeon, Eun-Seok; Kim, Jae-Joong; Cho, Myeong-Chan; Chae, Shung Chull; Oh, Byung-Hee; Kang, Seok-Min | Yonsel Univ, Coll Med, Cardiovasc Res Inst, Severance Cardiovasc Hosp,Div Cardiol,Dept Intern, Seoul 03722, South Korea; Seoul Natl Univ, Div Cardiol, Bundang Hosp, Seongnam, South Korea; Asan Med Ctr, Div Cardiol, Seoul, South Korea; Seoul Natl Univ Hosp, Dept Internal Med, Seoul, South Korea; Sungkyunkwan Univ, Dept Internal Med, Coll Med, Seoul, South Korea; Chungbuk Natl Univ, Dept Internal Med, Coll Med, Cheongju, South Korea; Chonnam Natl Univ, Dept Internal Med, Gwangju, South Korea; Yonsei Univ, Dept Internal Med, Wonju Coll Med, Wonju, South Korea; Catholic Univ Korea, Dept Internal Med, Seoul, South Korea; Kyungpook Natl Univ, Dept Internal Med, Coll Med, Daegu, South Korea; Mediplex Sejong Hosp, Dept Internal Med, Incheon, South Korea | Choi, Joon/D-6140-2017; Choi, Dong-Ju/J-5686-2012; Oh, Byung-Hee/G-9875-2011; Lee, Jeong Hoon/AAF-2400-2020; Jeong, Gi/AAB-2830-2021; Kim, Soo-Yeon/ADR-9663-2022; LEE, JI/L-6920-2013 | 12775923700; 56812485300; 35799900000; 57207065107; 57212315719; 35285421400; 15848011800; 56151235500; 7402426370; 56150430800; 7102851884; 35274349200; 7201371594; 7004279641; 36065764100; 7401727518; 7101962036; 57216293873; 7405685375 | smkang@yuhs.ac; | ESC HEART FAILURE | ESC HEART FAIL | 2055-5822 | 9 | 2 | SCIE | CARDIAC & CARDIOVASCULAR SYSTEMS | 2022 | 3.8 | 38.1 | 1.99 | 2025-06-25 | 15 | 17 | Vericiguat; Heart failure; Eligibility; Clinical pharmacology; Registries | Clinical pharmacology; Eligibility; Heart failure; Registries; Vericiguat | Heart Failure; Heterocyclic Compounds, 2-Ring; Hospital Mortality; Humans; Prospective Studies; Pyrimidines; Stroke Volume; Ventricular Function, Left; amino terminal pro brain natriuretic peptide; angiotensin receptor antagonist; beta adrenergic receptor blocking agent; brain natriuretic peptide; dipeptidyl carboxypeptidase inhibitor; enkephalinase inhibitor; hemoglobin; mineralocorticoid antagonist; potassium; sodium; vericiguat; fused heterocyclic rings; pyrimidine derivative; vericiguat; aged; Article; chronic kidney failure; cohort analysis; controlled study; diastolic blood pressure; disease registry; drug indication; estimated glomerular filtration rate; female; heart failure with reduced ejection fraction; heart rate; hospital admission; human; Korean (people); major clinical study; male; multicenter study; patient selection; prospective study; systolic blood pressure; heart failure; heart left ventricle function; heart stroke volume; hospital mortality; physiology | English | 2022 | 2022-04 | 10.1002/ehf2.13837 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Tuning the catalytic properties for cycloaddition of CO2 to propylene oxide on zeolitic-imidazolate frameworks through variation of structure and chemical composition | A series of Zn- and Co containing zeolitic-imidazolate frameworks (ZIFs), such as ZIF-8(Zn)/ZIF-67(Co), MAF-5 (Zn)/MAF-5(Co), and MAF-6(Zn)/MAF-6(Co), were investigated as catalysts for the synthesis of propylene carbonate (PC) from propylene oxide (PO) and CO2. The effect of the chemical composition, textural, structural and physicochemical properties on their activity was investigated. It was found that the activity of Zn-containing materials was higher in comparison with Co-based materials. Moreover, the yield of a PC was decreased with increasing Co content in structure of mixed Zn,Co-ZIFs (ZIF-8/ZIF-67) that was related to the accessibility of active centers to reagents and the strength of their interaction with CO2 and propylene oxide. The catalytic performance of Zn-ZIFs was found to improve with increasing the size of pore aperture due to the guest molecule facile diffusion and accessibility of the active site for reagent. In general, based on the results of the present work, the Zn-ZIFs could be considered as promising catalysts for cycloaddition of CO2 to epoxides and thereby opens new paths for further research. | Timofeeva, M. N.; Lukoyanov, I. A.; Panchenko, V. N.; Shefer, K., I; Mel'gunov, M. S.; Bhadra, B. N.; Jhung, S. H. | Boreskov Inst Catalysis SB RAS, Prospekt Akad Lavrentieva 5, Novosibirsk 630090, Russia; Novosibirsk State Tech Univ, Prospekt K Marksa 20, Novosibirsk 630092, Russia; Kyungpook Natl Univ, Dept Chem, DaeHak Ro 80, Daegu 41566, South Korea; Kyungpook Natl Univ, Green Nano Mat Res Ctr, DaeHak Ro 80, Daegu 41566, South Korea | Lukoyanov, Ivan/ABE-9113-2021; Jhung, Sung/AAO-6683-2021; Panchenko, Valentina/D-1068-2014; Timofeeva, Maria/E-9277-2012; Mel'gunov, Maxim/F-6554-2013; Bhadra, Biswa/ABG-6935-2021; Bhadra, Biswa Nath/ABG-6935-2021; Mel'gunov, Maksim/F-6554-2013 | 7005428141; 57214319427; 7102133466; 16231089500; 6602537021; 56982844300; 6701659467 | timofeeva@catalysis.ru;sung@knu.ac.kr; | MOLECULAR CATALYSIS | MOL CATAL | 2468-8231 | 529 | SCIE | CHEMISTRY, PHYSICAL | 2022 | 4.6 | 38.2 | 1.3 | 2025-06-25 | 16 | 17 | Zeolitic imidazolate frameworks; Catalytic properties; Propylene carbonate; CO2; Propylene oxide | METAL-ORGANIC FRAMEWORKS; CRYSTAL-GROWTH; CARBON-DIOXIDE; HIGH-PRESSURE; ZIF-8; PERFORMANCE; CONVERSION; FIXATION; PHASE; SIZE | Catalytic properties; CO<sub>2</sub>; Propylene carbonate; Propylene oxide; Zeolitic imidazolate frameworks | Catalysts; Physicochemical properties; Propylene; Zinc compounds; Catalytic properties; Chemical compositions; Cycloadditions; Physicochemical property; Propylene carbonate; Propylene oxide; Textural properties; Zeolitic imidazolate framework-8; Zeolitic imidazolate frameworks; ]+ catalyst; Carbon dioxide | English | 2022 | 2022-08 | 10.1016/j.mcat.2022.112530 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | Meeting Abstract | Antiosteoclastogenic active compounds from the roots of Broussonetia kazonoki | Vu, N. K.; Kim, J. A.; Min, B. S. | Daegu Catholic Univ, Gyongsan, South Korea; Kyungpook Natl Univ, Daegu, South Korea | khanh, vu/JXL-3187-2024 | PLANTA MEDICA | PLANTA MED | 0032-0943 | 1439-0221 | 88 | 15 | SCIE | CHEMISTRY, MEDICINAL;INTEGRATIVE & COMPLEMENTARY MEDICINE;PHARMACOLOGY & PHARMACY;PLANT SCIENCES | 2022 | 2.7 | 38.3 | 0 | English | 2022 | 2022-12 | 10.1055/s-0042-1759083 | 바로가기 | 바로가기 | 바로가기 | |||||||||||||
| ○ | ○ | Article | Circular Noncoding RNA hsacᵢᵣc₀₀₀₃₅₇₀ as a Prognostic Biomarker for Hepatocellular Carcinoma | Circular RNAs (circRNAs) are potential biomarkers owing to their stability, tissue specificity, and abundance. This study aimed to evaluate the clinical significance of hsa_circ_0003570 expression and to investigate its potential as a biomarker in hepatocellular carcinoma (HCC). We evaluated hsa_circ_0003570 expression in 121 HCC tissue samples, its association with clinicopathological characteristics, and overall and progression-free survival. Hsa_circ_0003570 expression was downregulated in HCC tissues. Low hsa_circ_0003570 expression was more common in tumors larger than 5 cm (odds ratio (OR), 6.369; 95% confidence interval (CI), 2.725-14.706; p 200 ng/mL; OR, 2.475; 95% CI, 1.159-5.291; p = 0.018). High hsa_circ_0003570 expression was an independent prognostic factor for overall survival (hazard ratio (HR), 0.541; 95% confidence interval (CI), 0.327-0.894; p = 0.017) and progression-free survival (HR, 0.633; 95% CI, 0.402-0.997; p = 0.048). Hsa_circ_0003570 is a potential prognostic biomarker in patients with HCC, and further validation of hsa_circ_0003570 is needed. | Jang, Se Young; Kim, Gyeonghwa; Tak, Won Young; Kweon, Young Oh; Lee, Yu Rim; Han, Young Seok; Han, Ja Ryung; Park, Jung Gil; Kang, Min Kyu; Lee, Hye Won; Lee, Won Kee; Park, Soo Young; Hur, Keun | Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Dept Internal Med, 130 Dongdeok Ro, Daegu 41944, South Korea; Kyungpook Natl Univ, Cell & Matrix Res Inst, Sch Med, Dept Biochem & Cell Biol, 680 Gukchaebosang Ro, Daegu 41944, South Korea; Daegu Catholic Univ, Dept Surg, Sch Med, 33 Duryugongwon Ro, Daegu 42472, South Korea; Yeungnam Univ, Dept Internal Med, Coll Med, 170 Hyonchung Ro, Daegu 42415, South Korea; Keimyung Univ, Dept Pathol, Sch Med, 1035 Dalgubeol Daero, Daegu 42601, South Korea; Kyungpook Natl Univ, Sch Med, Dept Med Informat, 680 Gukchaebosang Ro, Daegu 41944, South Korea; Kyungpook Natl Univ, Sch Med, Dept Biomed Sci, BK21 FOUR KNU Convergence Educ Program Biomed Sci, 680 Gukchaebosang Ro, Daegu 41944, South Korea | ; Kang, Min Kyu/U-8050-2018; Hur, Keun/G-9513-2011; Kim, Gyeonghwa/AAQ-6424-2021; Park, Jung/AAK-5167-2020; Kang, Min/U-8050-2018 | 57202881977; 57195957884; 7004074582; 7004694832; 57194094753; 7404096216; 57214671308; 57216816399; 59142854300; 57907713300; 22953484700; 57191674344; 8861888000 | psyoung0419@gmail.com;keunhur@knu.ac.kr; | GENES | GENES-BASEL | 2073-4425 | 13 | 8 | SCIE | GENETICS & HEREDITY | 2022 | 3.5 | 38.3 | 0.74 | 2025-06-25 | 7 | 7 | circular RNA; epigenetics; hepatocellular carcinoma; survival; progression; biomarker; prognosis | MICRORNA | biomarker; circular RNA; epigenetics; hepatocellular carcinoma; prognosis; progression; survival | Biomarkers, Tumor; Carcinoma, Hepatocellular; Humans; Liver Neoplasms; Prognosis; RNA; RNA, Circular; RNA, Untranslated; alpha fetoprotein; biological marker; circular ribonucleic acid; untranslated RNA; RNA; tumor marker; untranslated RNA; adult; Article; cancer prognosis; cancer staging; chronic hepatitis B; female; gene expression; human; human tissue; liver cell carcinoma; major clinical study; male; overall survival; progression free survival; tumor volume; genetics; liver tumor; metabolism; pathology; prognosis | English | 2022 | 2022-08 | 10.3390/genes13081484 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | HPRT1 Most Suitable Reference Gene for Accurate Normalization of mRNA Expression in Canine Dermal Tissues with Radiation Therapy | Reference genes are crucial in molecular biological studies as an internal control for gene re-search as they exhibit consistent expression patterns across many tissue types. In canines, radiation therapy is the most important therapeutic tool to cure various diseases like cancer. However, when using radiation for therapeutic strategy, radiation exposure to healthy tissues leads to some possible side effects such as acute radiation-induced skin injury and alters gene expression. Therefore, the analysis of a change in reference gene expression during the skin recovery process after radiation therapy is essential in healthy canine tissue. In the present study, we analyzed eight reference genes (ACTB, GAPDH, YWHAZ, GUSB, HPRT1, RPL4, RPS5, and TBP) in canine dermal tissues at 0, 1, 2, 3, 4, 5, 7, and 9 weeks of radiation exposure that affected the skin condition of canines. The stability of reference genes is determined by evaluating radiation therapy's effect on healthy canine dermal tissue. Epidermal marker, Keratin 10 expression varies each week after irradiation, and HPRT1 is found to be the most suitable for normalization of mRNA expression in radiation-exposed canine dermal tissues. Changes in the gene expression level were evaluated by using a reliable tool such as quantitative real-time polymerase chain reaction (qRT-PCR). In order to achieve a valid qRT-PCR result, the most stable reference genes used for normalization after the radiation exposure process are important. Therefore, the current study was designed to evaluate the most stable reference gene for the post-irradiation canine tissues. After radiation exposure, the alternation of reference gene expression was estimated by three algorithms (geNorm, Normfinder, and Bestkeeper). The RG validation programs (GeNorm and NormFinder) suggested that HPRT1, RPL4, and TBP were suitable for normalization in qRT-PCR. Furthermore, three algorithms suggested that HPRT1 was the most stable reference gene for normalization with qRT-PCR results, regardless of before and after radiation exposure. Whereas GAPDH was found to be the most unstable reference gene. In addition, the use of stable or unstable reference genes for the normalization of Keratin 10 expression showed statistical differences. Therefore, we observed that, to obtain accurate and suitable PCR results of the canine tissues with and without radiation exposure, the HPRT1 reference gene is recommended for normalization with its high stability. Additionally, the use of RGs such as HPRT1, RPL4, and TBP for normalization in qRT-PCR experiments is recommended for post-radiation canine tissues to generate more accurate and reliable data. These results will provide fundamental information regarding internal controls for gene expression studies and can be used for the analysis of gene patterns in regenerative medicine. | Lee, Sang-Yun; Choe, Yong-Ho; Han, Jang-Ho; Hwang, Gunha; Choi, Moon-Yeong; Thakur, Gitika; Jo, Chan-Hee; Oh, Seong-Ju; Lee, Won-Jae; Rho, Gyu-Jin; Lee, Sung-Lim; Hwang, Tae-Sung | Gyeongsang Natl Univ, Coll Vet Med, Jinju 52828, South Korea; Yangsan S Anim Canc Ctr, Yangsan 50638, South Korea; Kyungpook Natl Univ, Coll Vet Med, Daegu 41566, South Korea; Gyeongsang Natl Univ, Res Inst Life Sci, Jinju 52828, South Korea; Gyeongsang Natl Univ, Inst Anim Med, Coll Vet Med, Jinju 52828, South Korea | ; lee, sang/Q-4650-2019; Kim, Gi/AAK-3377-2021 | 57216913305; 57202927556; 57220161355; 57220815750; 57194007368; 57216199962; 57221808828; 57220159013; 57205486455; 6603725057; 16678898100; 56959822600 | sllee@gnu.ac.kr;hwangts@gnu.ac.kr; | GENES | GENES-BASEL | 2073-4425 | 13 | 11 | SCIE | GENETICS & HEREDITY | 2022 | 3.5 | 38.3 | 0.42 | 2025-06-25 | 5 | 4 | radiation therapy; canines; reference genes; HPRT1 | REAL-TIME; IONIZING-RADIATION; HOUSEKEEPING GENES; SKIN; IRRADIATION; KERATIN; INTEGRITY; SELECTION; CELLS; DOGS | canines; HPRT1; radiation therapy; reference genes | Algorithms; Animals; Dogs; Keratin-10; Real-Time Polymerase Chain Reaction; RNA, Messenger; 60S ribosomal protein L4; beta actin; beta glucuronidase; cytokeratin 10; homodimer; hypoxanthine phosphoribosyltransferase; messenger RNA; propofol; Ribosomal Protein S5; unclassified drug; cytokeratin 10; messenger RNA; algorithm; animal experiment; Article; dog; endotracheal intubation; gene amplification; gene expression; human; irradiation; nonhuman; polymerase chain reaction; punch biopsy; radiation dose distribution; radiation exposure; real time polymerase chain reaction; regenerative medicine; reverse transcription polymerase chain reaction; RNA extraction; RNA isolation; skin injury; algorithm; animal; genetics; metabolism; procedures | English | 2022 | 2022-11 | 10.3390/genes13111928 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Induced host defense by virulence manipulation of Erysiphe orontii through exogenous application of apoplastic nutrients | Host apoplastic nutrients might influence the proliferating biotroph, therefore, we analyzed the effect of host apoplastic nutrients on the virulence strategy of E. orontii (a biotroph model). E. orontii was initially treated with the analogs of commonly found apoplastic nutrients in a culture media. The analogs included Cyanoalanine (CAL), Trehalose, H2O2, acidic water (0.3% H3PO4), NaNO3, and MgSO4. After pre-treatment with the analogs of apoplastic nutrients, E. orontii was then inoculated to the host plants. After 5 and 8 days of post-infection (dpi), the virulence of E. orontii was determined through the expression of 20 virulence factor and 7 host defence genes. The expression of AVRk1, Ekal1, Ekal2, Ekal3, Ekal4, Ekal5, Ekal6, Ekal7, Ekal8, Epul5, Epul6, Epul7, Epul8, Epul9, Epul10, Epul11, Epul12, Epul5, Epul17 was determined as a virulence factor and expression of PR1, ERF1, PDF1.2a, PMRs, DMRs, IOS1, MYB3R4 was determined as host defence genes. The results revealed that the tested analogs vigorously affected the phenotypic and genotypic properties of E. orontii and thus its biotrophic virulence strategy. Certainly, the exogenous application of fungal growth-promoting apoplastic nutrients (CAL or TRE) severely reduced the virulence of E. orontii. Contrarily, the stress-inducing apoplastic analogs (H2O2 or acidic water) surprisingly increased the virulence of E. orontii. Moreover, the application of NaNO3 or MgSO4 decoyed the fungal growth and thus decreasing the E. orontii virulence. | Yousaf, Muhammad Junaid; Hussain, Anwar; Humayun, Muhammad; Iqbal, Amjad; Ali, Fawad; Lee, In-Jung; El-Sabrout, Ahmed M.; Elansary, Hosam O. | Abdul Wali Khan Univ Mardan, Dept Bot, Garden Campus, Mardan, Khyber Pakhtunk, Pakistan; Abdul Wali Khan Univ Mardan, Dept Food Sci & Technol, Garden Campus, Mardan, Khyber Pakhtunk, Pakistan; Bacha Khan Univ Charsadda, Dept Biotechnol, Charsadda, Khyber Pakhtunk, Pakistan; Kyungpook Natl Univ, Dept Appl Biosci, Daegu, South Korea; Alexandria Univ, Fac Agr El Shatby, Dept Appl Entomol & Zool, Alexandria 21545, Egypt; King Saud Univ, Coll Food & Agr Sci, Plant Prod Dept, Riyadh 11451, Saudi Arabia | ; Elansary, Hosam/E-3684-2019; Iqbal, Amjad/E-2010-2014; Lee, In-Jung/GLS-0432-2022; Humayun, Muhammad/M-6632-2015; Ali, Fawad/JCE-0794-2023; Hussain, Anwar/N-1605-2019 | 57739465900; 35621772200; 59140232300; 10142205100; 59784973200; 16425830900; 57202835194; 35388049900 | junaidyousaf44@gmail.com;drhussain@awkum.edu.pk;ijlee@knu.edu.kr;helansary@ksu.edu.sa; | PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | PHYSIOL MOL PLANT P | 0885-5765 | 119 | SCIE | PLANT SCIENCES | 2022 | 2.7 | 38.3 | 0.1 | 2025-06-25 | 0 | 1 | Biotroph; Exogenous treatment; Apoplastic nutrients; E; orontii; Virulence | POWDERY MILDEW; PLANTS | Apoplastic nutrients; Biotroph; E. orontii; Exogenous treatment; Virulence | English | 2022 | 2022-05 | 10.1016/j.pmpp.2022.101831 | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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