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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | ○ | Article | Chromosome characterization and physical mapping of 18S rDNA in Lilium longiflorum originated interspecific hybrids using combined genomic and fluorescent in situ hybridization | This study was aimed to differentiate parental genomes, examining intergenomic composition, and mapping mitotic metaphase chromosomes by localizing parental and 18S rDNA probes in seven interspecific hybrid progenies that originated from Lilium longiflorum. Since single method of in situ hybridization has been widely used in interspecific lily breeding, while in this study, flow cytometry was used in conjunction with genomic and fluorescent in situ hybridization to determine the genomic contribution of each parent to the interspecific progenies. A significant variation was observed in the DNA content, chromosome length, and 18S loci in F-1 as compared to the female and male parents. L. longiflorum showed nearly two times higher DNA content than the male parents and L. longiflorum x Asiatic progenies, but eight times higher than L. longiflorum x L. hansonii. Genomic in situ hybridization results revealed that both female and male parents contributed an equal number of chromosomes to their interspecific F-1 offspring. Fluorescent in situ hybridization mapping revealed that 18S rDNA had 8, 6 and 7 loci in L. longiflorum parents, i.e., White heaven, Bright tower, and White tower, respectively, whereas each Asiatic cultivar and L. hansonii used as male showed 8 and 12 loci respectively. Interspecific progenies showed 8 and 7 loci in LA, and 10-11 in LM hybrids. These cytogenetic results implied equal genetic and chromosomal contribution from both parents to their intergenomic progenies. Therefore, this combined cytogenetic method has the potential to be genome specific and time-saving approach in lily breeding that could determine the status of hybrids and their genomic origin while achieving physical mapping and detecting genes in different genomes. | Islam, Md Mazharul; Lee, Hyemin; Deepo, Deen Mohammad; Yesmin, Reshma; Ramzan, Fahad; Kim, Hong-Yul; Lim, Ki-Byung | Kyungpook Natl Univ, Dept Hort Sci, Daegu 41566, South Korea; Kyungpook Natl Univ, Inst Agr Sci & Technol, Daegu 41566, South Korea | Islam, Mazharul/AFS-6460-2022; Islam, Md Mazharul/AFS-6460-2022 | 57224982409; 57739404200; 57216909682; 57216450296; 56483068500; 56941037200; 7403175915 | kblim@knu.ac.kr; | EUPHYTICA | EUPHYTICA | 0014-2336 | 1573-5060 | 218 | 9 | SCIE | AGRONOMY;HORTICULTURE;PLANT SCIENCES | 2022 | 1.9 | 34.7 | 0.11 | 2025-06-25 | 1 | 1 | Metaphase; Chromosome; Lilium hansonii; Genetic loci; rDNA probes; Flow cytometry | KARYOTYPE ANALYSIS; CYTOGENETIC ANALYSIS; LILY CULTIVARS; GISH; RECOMBINATION; PROGENIES; HANSONII; GROWTH; FISH | Chromosome; Flow cytometry; Genetic loci; Lilium hansonii; Metaphase; rDNA probes | English | 2022 | 2022-09 | 10.1007/s10681-022-03066-0 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Reactivation of Bone Lining Cells are Attenuated Over Repeated Anti-sclerostin Antibody Administration | Reactivation of bone lining cells (BLCs) is a crucial mechanism governing the anabolic action of anti-sclerostin antibody (Scl-Ab) via modeling-based bone formation; however, it remains unclear whether this reactivation can be attenuated after persistent administration of Scl-Ab. Here, we aimed to investigate the reproducibility of persistent Scl-Ab administration for the reactivation of BLCs, and to elucidate the relationship between the activity of BLCs and serum levels of N-terminal procollagen type I (P1NP) during chronic Scl-Ab administration. We conducted an osteoblast lineage tracing study. Briefly, Dmp1-CreERt2(+):Rosa26R mice were injected with 1 mg of 4-hydroxy-tamoxifen weekly from postnatal weeks four to eight. Mice were treated twice with either vehicle or Scl-Ab (25 mg/kg) at weeks 12, 16, and 20, and were euthanized at weeks 8, 12, 13, 16, 17, 20, and 21 (4-6 mice in each group). After euthanization, the number and thickness of X-gal (+) cells on the periosteum of the femoral bones and the serum levels of P1NP were quantified at each time point. Scl-Ab induced a significant increase in the thickness of X-gal (+) cells on periosteal bone surfaces at postnatal weeks 13 (after 1st dose), 17 (after 2nd dose), and 21 (after 3rd dose) compared to that in vehicle-treated mice (all P < 0.001). In the Scl-Ab group, significant increases in the thickness of labeled cells were observed between weeks 16 and 17 and weeks 20 and 21 (both P < 0.001). The percentage increase in X-gal (+) cell thickness was 108.9% from week 12 to week 13, 54.6% from week 16 to week 17, and 49.2% from week 20 to week 21 in the Scl-Ab group. Although Scl-Ab treatment increased the serum levels of P1NP at postnatal weeks 13 and 17 compared with those at week 12 (P = 0.017 and P = 0.038, respectively), the same was not observed at week 21 (P = 0.296). A significant increase in P1NP levels was observed between weeks 16 and 17 and weeks 20 and 21 in the Scl-Ab group (P = 0.005 and P = 0.007, respectively). The percentage increase in P1NP levels was 141.7% from weeks 12 to 13, 114.8% from weeks 16 to 17, and 99.4% from weeks 20 to 21. Serum P1NP levels were positively correlated with X-gal (+) cell thickness (R-2 = 0.732, P < 0.001). Reactivation of BLCs is modestly attenuated, but reproducible, during persistent Scl-Ab administration. Serum P1NP levels appear to be an indicator of the impact of Scl-Ab on the conversion of BLCs into mature osteoblasts on periosteal bone surfaces, thus contributing to modeling-based bone formation. | Hong, A. Ram; Yang, Jae-Yeon; Lee, Ji Yeon; Suh, Joonho; Lee, Yun-Sil; Kim, Jung-Eun; Kim, Sang Wan | Chonnam Natl Univ, Sch Med, Dept Internal Med, Gwangju, South Korea; Seoul Natl Univ Hosp, Biomed Res Inst, Dept Res & Expt, Seoul, South Korea; Seoul Natl Univ, Dept Mol Genet, Sch Dent, Seoul, South Korea; Seoul Natl Univ, Dent Res Inst, Seoul, South Korea; Kyungpook Natl Univ, Cell & Matrix Res Inst, Sch Med, Dept Mol Med, Daegu, South Korea; Seoul Natl Univ, Coll Med, Dept Internal Med, 20 Boramae Ro 5 Gil, Seoul 07061, South Korea; Boramae Med Ctr, 20 Boramae Ro 5 Gil, Seoul 07061, South Korea | Kim, Sang Wan/J-5399-2012; Kim, Sang/J-5399-2012 | 56521082900; 14018662000; 57216774221; 57210638674; 57226670628; 57209054588; 14819695800 | swkimmd@snu.ac.kr; | CALCIFIED TISSUE INTERNATIONAL | CALCIFIED TISSUE INT | 0171-967X | 1432-0827 | 111 | 5 | SCIE | ENDOCRINOLOGY & METABOLISM | 2022 | 4.2 | 34.8 | 0.25 | 2025-06-25 | 2 | 2 | Sclerostin antibody; Bone lining cell; Modeling-based bone formation; P1NP | INTERMITTENT PARATHYROID-HORMONE; POSTMENOPAUSAL WOMEN; OSTEOPOROSIS; ROMOSOZUMAB; EXPRESSION; PREVENTION; MECHANISMS; DIAGNOSIS | Bone lining cell; Modeling-based bone formation; P1NP; Sclerostin antibody | Animals; Antibodies; Mice; Osteoblasts; Osteocytes; Osteogenesis; Periosteum; Reproducibility of Results; afimoxifene; procollagen C proteinase; romosozumab; antibody; animal experiment; animal tissue; Article; bone surface density; bone volume fraction; cell activation; cell differentiation; cell lineage; cell surface; controlled study; cortical thickness (bone); dual energy X ray absorptiometry; enzyme blood level; experimental design; female; male; micro-computed tomography; monoclonal antibody therapy; morphometry; mouse; nonhuman; ossification; osteoblast; periosteum; quantitative analysis; reproducibility; subcutaneous immunotherapy; surface property; trabecular number; trabecular separation; trabecular thickness; animal; bone development; metabolism; osteocyte | English | 2022 | 2022-11 | 10.1007/s00223-022-01013-8 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | The Korean undiagnosed diseases program phase I: expansion of the nationwide network and the development of long-term infrastructure | Background Phase I of the Korean Undiagnosed Diseases Program (KUDP), performed for 3 years, has been completed. The Phase I program aimed to solve the problem of undiagnosed patients throughout the country and develop infrastructure, including a data management system and functional core laboratory, for long-term translational research. Herein, we share the clinical experiences of the Phase I program and introduce the activities of the functional core laboratory and data management system. Results During the program (2018-2020), 458 patients were enrolled and classified into 3 groups according to the following criteria: (I) those with a specific clinical assessment which can be verified by direct testing (32 patients); (II) those with a disease group with genetic and phenotypic heterogeneity (353 patients); and (III) those with atypical presentations or diseases unknown to date (73 patients). All patients underwent individualized diagnostic processes based on the decision of an expert consortium. Confirmative diagnoses were obtained for 242 patients (52.8%). The diagnostic yield was different for each group: 81.3% for Group I, 53.3% for Group II, and 38.4% for Group III. Diagnoses were made by next-generation sequencing for 204 patients (84.3%) and other genetic testing for 35 patients (14.5%). Three patients (1.2%) were diagnosed with nongenetic disorders. The KUDP functional core laboratory, with a group of experts, organized a streamlined research pipeline covering various resources, including animal models, stem cells, structural modeling and metabolic and biochemical approaches. Regular data review was performed to screen for candidate genes among undiagnosed patients, and six different genes were identified for functional research. We also developed a web-based database system that supports clinical cohort management and provides a matchmaker exchange protocol based on a matchbox, likely to reinforce the nationwide clinical network and further international collaboration. Conclusions The KUDP evaluated the unmet needs of undiagnosed patients and established infrastructure for a data-sharing system and future functional research. The advancement of the KUDP may lead to sustainable bench-to-bedside research in Korea and contribute to ongoing international collaboration. | Kim, Soo Yeon; Lee, Seungbok; Woo, Hyewon; Han, Jiyeon; Ko, Young Jun; Shim, Youngkyu; Park, Soojin; Jang, Se Song; Lim, Byung Chan; Ko, Jung Min; Kim, Ki Joong; Cho, Anna; Kim, Hunmin; Hwang, Hee; Choi, Ji Eun; Kim, Man Jin; Moon, Jangsup; Seong, Moon-Woo; Park, Sung Sup; Choi, Sun Ah; Lee, Ji Eun; Kwon, Young Se; Sohn, Young Bae; Kim, Jon Soo; Kim, Won Seop; Lee, Yun Jeong; Kwon, Soonhak; Kim, Young Ok; Kook, Hoon; Cho, Yong Gon; Cheon, Chong Kun; Kang, Ki-Soo; Song, Mi-Ryoung; Kim, Young-Joon; Cha, Hyuk-Jin; Choi, Hee-Jung; Kee, Yun; Park, Sung-Gyoo; Baek, Seung Tae; Choi, Murim; Ryu, Dong-Sung; Chae, Jong-Hee | Seoul Natl Univ Hosp, Dept Gen Med, Seoul, South Korea; Seoul Natl Univ Childrens Hosp, Natl Univ Coll Med, Pediat Clin Neurosci Ctr, Dept Pediat, 101 Daehakro, Seoul 110744, South Korea; Seoul Natl Univ, Bundang Hosp, Dept Pediat, Seongnam, South Korea; Korea Univ, Ansan Hosp, Dept Pediat, Ansan, South Korea; Seoul Natl Univ, Coll Med, Div Clin Genet, Dept Pediat, Seoul, South Korea; SMG SNU Boramae Hosp, Dept Pediat, Seoul, South Korea; Seoul Natl Univ Hosp, Dept Lab Med, Seoul, South Korea; Ehwa Womans Univ Mokdong Hosp, Mokdong Hosp, Coll Med, Dept Pediat, Seoul, South Korea; Inha Univ, Inha Univ Hosp, Coll Med, Dept Pediat, Incheon, South Korea; Ajou Univ, Ajou Univ Hosp, Sch Med, Dept Med Genet, Suwon, South Korea; Chungbuk Natl Univ Hosp, Dept Pediat, Cheongju, South Korea; Chungbuk Natl Univ, Coll Med, Dept Pediat, Cheongju, South Korea; Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Dept Pediat, Daegu, South Korea; Chonnam Natl Univ, Sch Med, Dept Pediat, Gwangju, South Korea; Jeonbuk Natl Univ, Sch Med, Dept Lab Med, Jeonju, South Korea; Pusan Natl Univ, Yangsan Hosp, Dept Pediat, Yangsan, South Korea; Jeju Natl Univ Hosp, Dept Pediat, Jeju, South Korea; Gwangju Inst Sci & Technol, Sch Life Sci, Gwangju, South Korea; Seoul Natl Univ, Coll Pharm, Seoul, South Korea; Seoul Natl Univ, Sch Biol Sci, Seoul, South Korea; Kangwon Natl Univ, Coll Biomed Sci, Div Biomed Convergence, Chunchon, South Korea; Pohang Univ Sci & Technol, Dept Life Sci, Pohang, South Korea; Seoul Natl Univ, Coll Med, Dept Biomed Sci, Seoul, South Korea; Theragen Bio Co Ltd, Seongnam, South Korea | Song, Mi Ryoung/KYR-5865-2024; Kim, Siwon/KHX-9078-2024; Cha, Hyuk-Jin/AAB-8508-2020; Woo, Hyewon/KOD-6614-2024; Lee, Eun/J-5594-2012; Cho, Yong/Q-9924-2016; Baek, Seung Tae/HLW-8566-2023; Kwon, Young Se/HGC-6038-2022; Cheon, Chong Kun/GLU-0305-2022; KIM, JOO YEOL/HRC-8018-2023; KIM, YOUNG JIN/E-9374-2011; Choi, Ji/J-5696-2012; Kim, Joonghee/C-9811-2014; Kim, Nam-Joong/J-2735-2012; Lee, Ji-Eun/I-4784-2013; Lee, Jieun/I-4784-2013; Choi, Murim/AAP-9120-2021; Kim, Sae/AAR-3907-2020; Kim, Sang-Ha/ACC-9085-2022; Ko, JM/J-5370-2012; Chae, Jong/J-5683-2012; Kee, Yun/LDE-8635-2024 | 57191544930; 57203597141; 57297897900; 57193320849; 57222982666; 57205656646; 57214361562; 56841065300; 7201984041; 55087371200; 7409326381; 56655964000; 36190828200; 8632587500; 56126693100; 57069209100; 55201409700; 12242712000; 8766621100; 57191539101; 56813210000; 55721981300; 56457557700; 55425902600; 36677553400; 55978748900; 55468232200; 57192207814; 7006255531; 57712996300; 26649396100; 56892514000; 59192931700; 57207442933; 8370914200; 57195770981; 36902160900; 8144213000; 7201371505; 36929149400; 57800276700; 16241023700 | chaeped1@snu.ac.kr; | ORPHANET JOURNAL OF RARE DISEASES | ORPHANET J RARE DIS | 1750-1172 | 17 | 1 | SCIE | GENETICS & HEREDITY;MEDICINE, RESEARCH & EXPERIMENTAL | 2022 | 3.7 | 34.8 | 0.7 | 2025-06-25 | 6 | 5 | Rare disease; Undiagnosed disease program; Translational research; Data sharing | DISCOVERY | Data sharing; Rare disease; Translational research; Undiagnosed disease program | Databases, Factual; Humans; Information Dissemination; Rare Diseases; Republic of Korea; Undiagnosed Diseases; cholinesterase inhibitor; corticosteroid; levodopa; mexiletine; midazolam; pamidronic acid; rituximab; Article; bone development; brain atrophy; chromosome analysis; clinical assessment; clinical decision making; cohort analysis; congenital myasthenic syndrome; data reanalysis; diagnostic value; drug withdrawal; dystonia; gene; genetic heterogeneity; genetic screening; gestational age; health program; high throughput sequencing; human; hyperreflexia; immunotherapy; intellectual impairment; ketogenic diet; Korea; long term care; lymphedema; macrocephaly; microarray analysis; muscle biopsy; muscle hypertonia; muscle weakness; muscular dystrophy; myositis; nuclear magnetic resonance imaging; osteogenesis imperfecta; phase 1 clinical trial (topic); phenotype; PIEZO1 gene; pleura effusion; polymerase chain reaction; RNA sequencing; scoliosis; seizure; spinal muscular atrophy; undiagnosed disease; whole genome sequencing; factual database; genetics; information dissemination; rare disease; South Korea | English | 2022 | 2022-10-08 | 10.1186/s13023-022-02520-5 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | A Comparative Immunohistochemical Study of Wound Healing after Dental Diode Laser Treatment in the Rat Oral Mucosa | This study aimed to examine the differences in healing patterns using two types of diode laser devices (laser A and laser B) and a steel scalpel for periodontal surgery through histological and immunohistochemical methods. Twenty 12-week-old male rats were assigned to three groups (3, 7, and 14 days). Square-shaped erosion wounds (2 x 2 mm(2) diameter) were created on the hard palate of each rat. Two wounds were created using Laser A and a steel scalpel (Bard-Parker No. 15) on the right palate and using Laser B and a steel scalpel on the left side. Rats were sacrificed after 3, 7, and 14 days. Tissues were collected with a margin of 1 mm from the border of the erosional wound of the maxillary hard palate. Histological and immunohistochemical analyses were performed on the tissue samples after 3, 7, and 14 days. The tissue healing pattern and expression of inducible nitric oxide synthase (iNOS) and cluster of differentiation (CD) were observed under a light microscope. Statistical analysis was conducted using the Kruskal-Wallis H test for comparison among the groups (alpha = 0.05). In comparison to the wounds made with the scalpel, wounds treated with lasers A and B showed delayed healing patterns. There was no significant difference between the two laser treatment groups (p > 0.05). The expression of iNOS and CD68 was not significantly different among the three groups after 3 and 7 days (p > 0.05). On day 14, the groups treated with the dental diode lasers showed higher expression than the group treated with the steel scalpel, but no significant difference was observed (p > 0.05). Laser-induced wounds tended to heal slower than surgical wounds performed using a steel scalpel, but histological and immunohistochemical results showed no significant difference between the dental diode laser and scalpel groups. | Kim, Hye Rin; Son, Keunbada; Son, Young-Tak; Kim, Yong-Gun; Lee, Kyu-Bok; Lee, Seung Cheol; Suh, Jo-Young; Lee, Jae Mok | Kyungpook Natl Univ, Sch Dent, Dept Periodontol, Daegu 41940, South Korea; Kyungpook Natl Univ, Adv Dent Device Dev Inst A3DI, Daegu 41940, South Korea; Kyungpook Natl Univ, Grad Sch, Dept Dent Sci, Daegu 41940, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Prosthodont, Daegu 41940, South Korea; Andong Gen Hosp, Dept Pathol, Andong Si 36743, South Korea | ; SON, Keunbada/AAG-8089-2019; Son, Keunbada/AAG-8089-2019 | 57902451200; 57202916520; 57219111205; 55622694400; 15925571200; 57902247700; 7201514992; 17346330000 | kblee@knu.ac.kr;leejm@knu.ac.kr; | BIOENGINEERING-BASEL | BIOENGINEERING-BASEL | 2306-5354 | 9 | 9 | SCIE | ENGINEERING, BIOMEDICAL | 2022 | 4.6 | 34.9 | 0.3 | 2025-06-25 | 3 | 4 | dental diode laser; histologic research; periodontal surgery; scalpel; wound healing | dental diode laser; histologic research; periodontal surgery; scalpel; wound healing | English | 2022 | 2022-09 | 10.3390/bioengineering9090466 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | A nationwide survey of the association between nonalcoholic fatty liver disease and the incidence of asthma in Korean adults | Background Asthma and nonalcoholic fatty liver disease (NAFLD) are chronic diseases known to be associated with metabolic abnormalities. We aimed to clarify the association between NAFLD and asthma incidence in a large population-based cohort. Methods and findings We selected 160,603 individuals without comorbidities from the National Health Insurance Service-National Sample cohort between 2009 and 2014. NAFLD was defined using a surrogate marker, fatty liver index (FLI). During a median of 5.08 years' follow-up, 16,377 subjects (10.2%) were newly diagnosed with asthma and categorized into three groups according to FLI. The cumulative incidence of asthma was higher in subjects with higher vs. lower FLIs (FLI = 60, 10.5%). Higher FLI was associated with an increased incidence of asthma (Hazard ratios (HR)(highest vs. lowest FLI), 1.25; 95% CI, 1.15-1.36). The results using another definition of NAFLD, as measured by the hepatic steatosis index (HSI), were similar to the primary results. This association was more pronounced in women than in men (HR 1.46; 95% CI, 1.13-1.64 vs. HR 1.07; 95% CI, 0.94-1.20). Conclusions This study demonstrated that NAFLD, as measured by FLI and HSI, may influence the incidence rates of asthma in adults, especially in women. | Roh, Jae-Hyung; Lee, Hanbyul; Yun-Jeong, Bae; Park, Chan Sun; Kim, Hyo-Jung; Yoon, Sun-Young | Chungnam Natl Univ, Chungnam Natl Univ Sejong Hosp, Sch Med, Dept Cardiol Internal Med, Sejong, South Korea; Kyungpook Natl Univ, Dept Stat, Daegu, South Korea; Asan Med Ctr, Asan Inst Life Sci, Hlth Innovat Bigdata Ctr, Seoul, South Korea; Inje Univ, Haeundae Paik Hosp, Dept Internal Med, Busan, South Korea; INJE Haeundae Paik Hosp, Div Pulmonol, Dept Internal Med, Busan, South Korea; Chungnam Natl Univ, Chungnam Natl Univ Sejong Hosp, Dept Allergy & Pulmonol Internal Med, Sejong, South Korea | Park, Chan-Sun/AAT-3431-2021 | 57216309871; 59107079300; 57424122800; 57191996109; 58368872200; 56452038900 | ysyoon@cnuh.co.kr; | PLOS ONE | PLOS ONE | 1932-6203 | 17 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2022 | 3.7 | 34.9 | 0.85 | 2025-06-25 | 13 | 12 | BODY-MASS INDEX; METABOLIC SYNDROME; OBESITY; PREVALENCE; RISK; METAANALYSIS; MORTALITY | Adult; Asthma; Biomarkers; Female; Health Surveys; Humans; Incidence; Liver; Male; Middle Aged; Non-alcoholic Fatty Liver Disease; Patient Acceptance of Health Care; Republic of Korea; Risk Factors; Sex Factors; biological marker; adult; Article; asthma; cerebrovascular disease; cohort analysis; comorbidity; controlled study; diabetes mellitus; disease association; disease classification; fatty liver index; female; follow up; health service; health survey; hepatic steatosis index; human; hypertension; incidence; Korean (people); major clinical study; male; middle aged; national health insurance; nonalcoholic fatty liver; patient selection; risk factor; sample size; sex difference; smoking; asthma; complication; health survey; incidence; liver; nonalcoholic fatty liver; pathology; patient attitude; sex factor; South Korea | English | 2022 | 2022-01-21 | 10.1371/journal.pone.0262715 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | An account of Colletotrichum species associated with anthracnose of Atractylodes ovata in South Korea based on morphology and molecular data | Ovate-leaf atractylodes (OLA) (Atractylodes ovata) is a well-known medicinal plant in Korea; its dried rhizome and root extracts are used in herbal medicine. However, anthracnose is a great challenge to the OLA cultivation in South Korea. Colletotrichum spp. is a major group of plant pathogens responsible for anthracnose on a range of economically important hosts. Its occurrence on OLA remains unresolved. To investigate the diversity, morphology, phylogeny, and biology of Colletotrichum spp., 32 fungal isolates were obtained from 30 OLA-affected leaves collected from five different farms, in two regions in South Korea, Mungyeong and Sangju. The phylogenetic analysis with four or five gene loci (ITS, TUB2, ACT, GAPDH, and CHS-1) along with morphology of 26 representative isolates delineated six previously known Colletotrichum species including C. fructicola, C. gloeosporioides sensu stricto (s.s), C. cigarro, C. plurivorum, C. siamense and C. sojae, and one new species, described here as C. ovataense. Amongst these species, C. gloeosporioides s.s. and C. plurivorum were the most prevalent species. A pathogenicity test on the detached leaves revealed that different Colletotrichum species presented a distinct degree of virulence, confirming Koch's postulates. In this study, C. fructicola, C. cigarro, C. plurivorum, C. siamense, and C. sojae were reported from A. ovata for the first time, as the causal agent of ovate-leaf atractylodes anthracnose. Understanding the diversity and biology of the Colletotrichum species population will help in managing this disease. | Hassan, Oliul; Kim, Ju Sung; Romain, Bekale Be Ndong Dimitri; Chang, Taehyun | Kyungpook Natl Univ, Dept Ecol Environm Syst, Coll Ecol Environm Sci, Sangju, Gyeongbuk, South Korea | Chang, Taehyun/S-1364-2019; Hassan, Oliul/AEB-6678-2022; Kim, Seong Cheol/ABD-1493-2022 | 12762464400; 57322992200; 57428350300; 57428252000; 55301101700 | thchang@knu.ac.kr; | PLOS ONE | PLOS ONE | 1932-6203 | 17 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2022 | 3.7 | 34.9 | 0.92 | 2025-06-25 | 14 | 14 | LEAF-SPOT; GLOEOSPORIOIDES COMPLEX; PRIMER SETS; 1ST REPORT; DISEASE; SYSTEMATICS; PERSIMMON; PATHOGEN; YIELD | Atractylodes; Colletotrichum; Genes, Fungal; Phylogeny; Plant Diseases; Plant Leaves; Republic of Korea; ACT gene; anthracnose; Article; Atractylodes lancea; CHS 1 gene; Colletotrichum; Colletotrichum cigarro; Colletotrichum fructicola; Colletotrichum ovataense; Colletotrichum plurivorum; Colletotrichum siamense; Colletotrichum sojae; controlled study; fungal virulence; fungus isolation; GAPDH gene; gene locus; Glomerella cingulata; ITS gene; nonhuman; phylogeny; plant gene; plant leaf; plant structures; South Korea; species diversity; TUB2 gene; Atractylodes; classification; fungal gene; genetics; growth, development and aging; microbiology; phylogeny; plant disease | English | 2022 | 2022-01-25 | 10.1371/journal.pone.0263084 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Review | Application of Sygen® in Diabetic Peripheral Neuropathies-A Review of Biological Interactions | This study investigates the role of Sygen (R) in diabetic peripheral neuropathy, a severe disease that affects the peripheral nervous system in diabetic individuals. This disorder often impacts the lower limbs, causing significant discomfort and, if left untreated, progresses into more serious conditions involving chronic ulcers and even amputation in many cases. Although there are management strategies available, peripheral neuropathies are difficult to treat as they often present multiple causes, especially due to metabolic dysfunction in diabetic individuals. Gangliosides, however, have long been studied and appreciated for their role in neurological diseases. The monosialotetrahexosylganglioside (GM1) ganglioside, popularly known as Sygen, provides beneficial effects such as enhanced neuritic sprouting, neurotrophism, neuroprotection, anti-apoptosis, and anti-excitotoxic activity, being particularly useful in the treatment of neurological complications that arise from diabetes. This product mimics the roles displayed by neurotrophins, improving neuronal function and immunomodulation by attenuating exacerbated inflammation in neurons. Furthermore, Sygen assists in axonal stabilization and keeps nodal and paranodal regions of myelin fibers organized. This maintains an adequate propagation of action potentials and restores standard peripheral nerve function. Given the multifactorial nature of this complicated disorder, medical practitioners must carefully screen the patient to avoid confusion and misdiagnosis. There are several studies analyzing the role of Sygen in neurological disorders. However, the medical literature still needs more robust investigations such as randomized clinical trials regarding the administration of this compound for diabetic peripheral neuropathies, specifically. | Coelho, Marcelo Amaral; Jeyaraman, Madhan; Jeyaraman, Naveen; Rajendran, Ramya Lakshmi; Sugano, Andre Atsushi; Mosaner, Tomas; Santos, Gabriel Silva; Bizinotto Lana, Joao Vitor; Lana, Anna Vitoria Santos Duarte; da Fonseca, Lucas Furtado; Domingues, Rafael Barnabe; Gangadaran, Prakash; Ahn, Byeong-Cheol; Lana, Jose Fabio Santos Duarte | Brazilian Inst Regenerat Med, Dept Orthopaed, BR-13334170 Indaiatuba, Brazil; Dr MGR Educ & Res Inst, Dept Orthopaed, Fac Med, Sri Lalithambigai Med Coll & Hosp, Chennai 600095, Tamil Nadu, India; Atlas Hosp, Dept Orthopaed, Tiruchirappalli 620002, Tamil Nadu, India; Kyungpook Natl Univ, Sch Med, Dept Nucl Med, Daegu 41944, South Korea; Ctr Univ Max Planck, Med Specialties Sch Ctr, BR-13343060 Indaiatuba, Brazil; Univ Fed Sao Paulo, Dept Orthopaed, BR-04024002 Sao Paulo, Brazil; Kyungpook Natl Univ, BK21 FOUR KNU Convergence Educ Program Biomed Sci, Dept Biomed Sci, Sch Med, Daegu 41944, South Korea | Jeyaraman, Madhan/ABB-8464-2020; Rajendran, Ramya/AAV-6338-2021; Gangadaran, Prakash/AAV-3102-2021 | 57715297300; 57216926503; 57219306833; 57195318729; 57715819300; 57217423329; 57204415693; 57716594400; 57423777300; 55953908300; 57715559200; 54393130400; 7202791511; 56275938500 | macamaral73@gmail.com;madhanjeyaraman@gmail.com;naveenjeyaraman@yahoo.com;ramyag@knu.ac.kr;drandresugano@gmail.com;contato@clinicaviveresanus.com.br;gabriel1_silva@hotmail.com;jvblana@gmail.com;annavitorialana1104@gmail.com;ffonsecalu@gmail.com;dr.rafael.itu@gmail.com;prakashg@knu.ac.kr;abc2000@knu.ac.kr;josefabiolana@gmail.com; | BIOENGINEERING-BASEL | BIOENGINEERING-BASEL | 2306-5354 | 9 | 5 | SCIE | ENGINEERING, BIOMEDICAL | 2022 | 4.6 | 34.9 | 0.07 | 2025-06-25 | 2 | 2 | diabetic peripheral neuropathy; gangliosides; sygen; neuroprotection; regenerative medicine | diabetic peripheral neuropathy; gangliosides; neuroprotection; regenerative medicine; sygen | English | 2022 | 2022-05 | 10.3390/bioengineering9050217 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Aspergillus violaceofuscus alleviates cadmium and chromium stress in Okra through biochemical modulation | Endophytic fungi from the Chilli were used to help okra plants exposed to cadmium (Cd) or chromium (Cr) stress. Initially, the strain Ch06 produced higher amounts of indole acetic acid (IAA) (230.5 mu g/mL), sugar (130.7 mu g/mL), proteins (128.2 mu g/mL), phenolics (525.6 mu g/mL) and flavonoids (98.4 mu g/mL) in Czapek broth supplemented with Cd or Cr. The production of IAA and other metabolites in such a higher concentration suggested that Ch06 might improve plant growth under heavy metal stress. For this reason, an experiment was designed, in which biomass of Ch06 (at 2g/100g of sand) were applied to the okra plants exposed to Cd or Cr stress (at 100 or 500 mu g/g). The results exhibited that Ch06 improved the total chlorophyll (36.4 +/- 0.2 SPAD), shoot length (22.6 +/- 0.2 cm), root length (9.1 +/- 0.6 cm), fresh weight (5 +/- 0.6 g), dry weight (1.25 +/- 0.01 g), sugars (151.6 mu g/g), proteins (114.8 mu g/g), proline (6.7 mu g/g), flavonoids (37.9 mu g/g), phenolics (70.7 mu g/g), IAA (106.7 mu g/g), catalase (0.75 enzyme units/g tissue) and ascorbic acid oxidaze (2.2 enzyme units/g tissue) of the associated okra plants. Similar observations have been recorded in Ch06 associated okra plants under Cd and Cr stress. Also, Ch06 association reduced translocation of Cd (35% and 45%) and Cr (47% and 53%) to the upper parts of the okra plants and thus reduced their toxicity. The internal transcribed spacer (ITS) region amplification of 18S rDNA (ribosomal deoxyribo nucleic acid) exhibited that the potent strain Ch06 was Aspergillus violaceofuscus. The results implied that A. violaceofuscus has the ability to promote host species growth exposed to Cd and Cr. Moreover, it helped the host plants to recover in Cd and Cr polluted soils, hence can be used as biofertilizer. | Aziz, Laila; Hamayun, Muhammad; Rauf, Mamoona; Iqbal, Amjad; Husssin, Anwar; Khan, Sumera Afzal; Shafique, Maryam; Arif, Muhammad; Ahmad, Ayaz; Rehman, Gauhar; Ali, Sajid; Kang, Sang Mo; Lee, In-Jung | Abdul Wali Khan Univ Mardan, Dept Bot, Mardan, Pakistan; Abdul Wali Khan Univ Mardan, Dept Food Sci & Technol, Mardan, Pakistan; Univ Peshawar, Ctr Biotechnol & Microbiol, Peshawar, Pakistan; Fed Urdu Univ Art Sci & Technol, Dept Microbiol, Karachi, Pakistan; Abdul Wali Khan Univ Mardan, Dept Biotechnol, Mardan, Pakistan; Abdul Wali Khan Univ Mardan, Dept Zool, Mardan, Pakistan; Yeumgnam Univ, Dept Hort & Life Sci, Gyongsan, South Korea; Kyungpook Natl Univ, Coll Agr & Life Sci, Dept Appl Biosci, Daegu, South Korea | ; rauf, mamoona/AAC-3246-2019; Kang, Sang-Mo/MBG-7823-2025; Ahmad, Ayaz/GVT-1025-2022; Hamayun, Muhammad/B-1694-2010; Arif, Muhammad/GNM-5831-2022; Rauf, Mamoona/AAC-3246-2019; Lee, In-Jung/GLS-0432-2022; Iqbal, Amjad/E-2010-2014; Ali, Sajid/GLS-7322-2022; Ayaz, Ahmad/GVT-1025-2022; Rehman, Gauhar/AAW-9173-2020; Khan, Shuhab/C-6839-2008 | 57222572212; 59297330100; 55609311400; 10142205100; 57222571663; 9636646900; 56598427200; 57202654530; 55420263200; 35734938700; 57214290889; 56189696900; 16425830900 | hamayun@awkum.edu.pk;ijlee@knu.ac.kr; | PLOS ONE | PLOS ONE | 1932-6203 | 17 | 10 | SCIE | MULTIDISCIPLINARY SCIENCES | 2022 | 3.7 | 34.9 | 0.78 | 2025-06-25 | 9 | 11 | PLANT-GROWTH; BIOREMEDIATION; ACCUMULATION; TOLERANCE; METALS | Abelmoschus; Ascorbic Acid; Aspergillus; Biodegradation, Environmental; Cadmium; Catalase; Chlorophyll; Chromium; DNA, Ribosomal; Flavonoids; Metals, Heavy; Nucleic Acids; Proline; Sand; Soil Pollutants; Sugars; ascorbate oxidase; cadmium; catalase; chlorophyll; chromium; DNA 18S; flavonoid; indoleacetic acid; internal transcribed spacer; phenol derivative; proline; protein; sugar; ascorbic acid; cadmium; carbohydrate; chromium; heavy metal; nucleic acid; ribosome DNA; Article; Aspergillus; Aspergillus violaceofuscus; biochemistry; concentration (parameter); controlled study; dry weight; fresh weight; heavy metal stress; host; metabolite; nonhuman; nucleotide sequence; okra; plant growth; plant stress; root length; sand; shoot length; Abelmoschus; Aspergillus; bioremediation; metabolism; soil pollutant | English | 2022 | 2022-10-14 | 10.1371/journal.pone.0273908 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Bioengineering of Hair Follicle-like Structure for Validation of Hair Growth Promoting Compounds | We aimed to establish screening and efficacy test techniques for use in the development of hair-promoting agents. To this end, we used the dermal papilla cell (DPc)-derived immortalized cell line (SV40T-hTERT DPc) and neonatal foreskin-derived keratinocyte cell line (Ker-CT) to form an immortalized cell-based hair follicle-like structure. The SV40T-hTERT DPc spheroids exhibited a higher cell ratio in the spheroids than primary DPc spheroids, and SV40T-hTERT DPc aggregated with spheroids larger in diameter than primary DPc when the same cell number was seeded into the low-adhesion plate. Microscopic imaging and fluorescence staining results indicated that both primary and immortalized cell combinations form a hair follicle-like structure with a long-stretched keratinocyte layer under the condition that the spheroids have the same diameter as that of in vivo dermal papillary tissue in the hair follicle. The hair follicle-like structure elongation was increased upon treatment with three known hair follicle growth-promoting compounds (minoxidil, tofacitinib, and ascorbic acid) compared with that in the control group. Therefore, using immortalized cells to generate a coherent follicle-like structure, we have developed models for screening and evaluating hair-care materials commonly used in the industry. | Joo, Hyun Woo; Kim, Min Kyu; Bak, Soon Sun; Sung, Young Kwan | Kyungpook Natl Univ, Sch Med, Dept Immunol, Daegu 41944, South Korea; Kyungpook Natl Univ, Sch Med, Dept Biomed Sci, BK21 FOUR KNU Convergence Educ Program Biomed Sci, Daegu 41944, South Korea | 55989818400; 57208648398; 41561065700; 55663365300 | ysung@knu.ac.kr; | BIOENGINEERING-BASEL | BIOENGINEERING-BASEL | 2306-5354 | 9 | 11 | SCIE | ENGINEERING, BIOMEDICAL | 2022 | 4.6 | 34.9 | 0.23 | 2025-06-25 | 2 | 3 | alopecia; hair follicles; hair loss; keratinocytes; screening; spheroids | alopecia; hair follicles; hair loss; keratinocytes; screening; spheroids | English | 2022 | 2022-11 | 10.3390/bioengineering9110645 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||||
| ○ | ○ | Article | Clinical and growth outcomes after meconium-related ileus improved with Gastrografin enema in very low birth weight infants | BackgroundMeconium-related ileus in very low birth weight infants can lead to increased morbidity or mortality and prolonged hospitalization without prompt diagnosis and treatment. This study primarily aimed to identify the incidence of and factors associated with meconium-related ileus and secondarily sought to investigate clinical and growth outcomes after water-soluble contrast media (Gastrografin) enema. MethodsWe retrospectively reviewed medical records of very low birth weight infants born between February 2009 and March 2019 in the neonatal intensive care unit of a single medical center. Perinatal factors, clinical outcomes, and growth outcomes were compared between the group with meconium-related ileus that received Gastrografin enema and the control group. ResultsTwenty-four (6.9%) patients were diagnosed with meconium-related ileus among 347 very low birth weight infants. All achieved successful evacuation of meconium with an average of 2.8 (range: 1-8) Gastrografin enema attempts without procedure-related complications. Initiation of Gastrografin enema was performed at mean 7.0 days (range: 2-16) after birth. Incidences of moderate to severe bronchopulmonary dysplasia were higher and the duration of mechanical ventilation and need for oxygen were longer in the meconium-related ileus group (P = 0.039, 0.046, 0.048, respectively). Meconium-related ileus infants took more time to start enteral feeding and the nothing per oral time was longer (P = 0.001 and 0.018, respectively). However, time to achieve full enteral feeding and Z-scores for weight and height at 37 weeks and at 6 months corrected age did not differ between the two groups. ConclusionsGastrografin enema in very low birth weight infants with meconium-related ileus was an effective and safe medical management. Following Gastrografin enema, very low birth weight infants with meconium-related ileus achieved similar subsequent feeding progress and similar growth levels as the control groups without meconium-related ileus. | Song, Woo Sun; Yoon, Hye Sun; Kim, Seung Yeon | Kyungpook Natl Univ, Coll Med, Dept Med, Daegu, South Korea; Eulji Univ, Nowon Eulji Med Ctr, Dept Pediat, Sch Med, Seoul, South Korea | 57204459654; 25032139400; 57226532914 | dunggiduk@eulji.ac.kr; | PLOS ONE | PLOS ONE | 1932-6203 | 17 | 8 | SCIE | MULTIDISCIPLINARY SCIENCES | 2022 | 3.7 | 34.9 | 0.28 | 2025-06-25 | 3 | 4 | PRETERM INFANTS; CONTRAST ENEMA; 1ST STOOL; OBSTRUCTION; BOWEL; EVACUATION; PREMATURITY; MANAGEMENT; DISORDERS; DIAGNOSIS | Diatrizoate Meglumine; Enema; Humans; Ileus; Infant, Newborn; Infant, Very Low Birth Weight; Intestinal Obstruction; Meconium; Retrospective Studies; meglumine diatrizoate plus sodium diatrizoate; enema; meglumine diatrizoate; adult; Article; artificial ventilation; body height; body weight; case control study; child growth; clinical article; clinical outcome; controlled study; disease association; disease severity; enteric feeding; female; health center; human; incidence; infant; lung dysplasia; male; meconium ileus; medical record review; neonatal intensive care unit; retrospective study; scoring system; very low birth weight; ileus; intestine obstruction; meconium; newborn; very low birth weight | English | 2022 | 2022-08-11 | 10.1371/journal.pone.0272915 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||||
| ○ | ○ | Article | Comparison of Osseointegration of Dental Implants Placed in Rabbit Tibia Using Two Dental Laser and Implant Handpiece Systems | The present study aimed to confirm the usefulness of a multi-laser handpiece system currently under development. Implants were placed in the tibia of rabbits using a conventional separate laser-implant handpiece system (control group; SurgicPro+; NSK, Kanuma, Japan and Epic 10; Biolase, Irvine, CA, USA) and a multi-laser handpiece system (experimental group; BLP 10; Saeshin, Daegu, Korea). Implants were placed in left and right tibias of five rabbits using a conventional laser-implant handpiece system and a multi-laser handpiece system (N = 5 per group). Subsequently, micro-computed tomography (micro-CT; bone-to-implant contact evaluation), implant stability quotient (ISQ) measurement, and histological evaluations were performed to confirm the implant placement results. The independent t-test and the paired t-test were used to compare the ISQ values and the results of the two implant-laser handpiece groups (alpha = 0.05), respectively. No statistically significant difference in micro-CT, ISQ, and histological evaluations was observed between implant placement by the two systems (p > 0.05) except implant initial stability. The use of the multi-laser handpiece system is expected to produce the same results as a conventional separate laser-implant handpiece system with the higher implant initial stability. Additionally, it will potentially make the clinical environment more pleasant and will provide convenience for the clinicians. | Park, Jin-Han; Son, Keunbada; Son, Young-Tak; Kim, Yong-Gun; Suh, Jo-Young; Lee, Kyu-Bok; Lee, Jae-Mok | Kyungpook Natl Univ, Sch Dent, Dept Periodontol, Daegu 41566, South Korea; Kyungpook Natl Univ, Adv Dent Device Dev Inst A3DI, Daegu 41566, South Korea; Kyungpook Natl Univ, Grad Sch, Dept Dent Sci, Daegu 41566, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Prosthodont, Daegu 41566, South Korea | ; SON, Keunbada/AAG-8089-2019; Son, Keunbada/AAG-8089-2019 | 57959011500; 57202916520; 57219111205; 55622694400; 7201514992; 15925571200; 17346330000 | kblee@knu.ac.kr;leejm@knu.ac.kr; | BIOENGINEERING-BASEL | BIOENGINEERING-BASEL | 2306-5354 | 9 | 11 | SCIE | ENGINEERING, BIOMEDICAL | 2022 | 4.6 | 34.9 | 0.08 | 2025-06-25 | 1 | 1 | dental implant; laser; implant stability; osseointegration | dental implant; implant stability; laser; osseointegration | English | 2022 | 2022-11 | 10.3390/bioengineering9110681 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Comparison of Seegene Anyplex II HPV28 assay with BD Onclarity HPV assay for human papillomavirus genotyping | Presently, human papillomavirus (HPV)-based cervical cancer screening is commonly used and is replacing conventional cytology screening tests. The HPV genotyping assay is useful for triage in cervical cancer screening and the evaluation of HPV vaccination effects. In this study, we evaluated the clinical performance of two HPV genotyping assays, BD Onclarity HPV (Onclarity) and Seegene Anyplex II HPV28 (Anyplex) in the detection of relevant cervical lesions and for HPV genotyping concordance. Anyplex and Onclarity assays were performed on 920 consecutive liquid-based specimens. Anyplex, sensitivity, specificity, and genotyping concordance with Onclarity were optimal when restricted to >= 2+ (medium) viral loads. HPV genotyping agreement between the two assays ranged between 0.75 and 0.9 (excellent), except for HPV 33/58, which was 0.73 (good). With Onclarity as a reference, the relative sensitivity of Anyplex for the detection of >= CIN 2 was 1.05 (95% CI: 0.99-1.1) and the relative specificity for detection of negative for intraepithelial lesion and malignancy (NILM) was 0.89 (95% CI: 0.85-0.93). For most >= CIN 2 lesions, high-risk HPV was detected by Onclarity (66/72) and Anyplex (69/72) assays. For high-risk HPV negative >= CIN 2 lesions, possible high-risk HPV genotypes were detected by Anyplex. In conclusion, the genotyping agreement between the tests was good to excellent. Full genotyping with Anyplex might confer additional benefits to patients with >= CIN 2, although the difference is small. We also suggest an optimal cutoff value when reporting HPV infections using the Anyplex assay (>= 2+; medium viral loads). | Kim, Moonsik; Kim, Jinhee; Park, Nora Jee-Young; Park, Ji Young | Kyungpook Natl Univ, Chilgok Hosp, Sch Med, Dept Pathol, Daegu, South Korea | Kim, Ju-Hyoung/N-1450-2019 | 57195918515; 57789253600; 57226185359; 57210160197 | jyparkmd@knu.ac.kr; | PLOS ONE | PLOS ONE | 1932-6203 | 17 | 7 | SCIE | MULTIDISCIPLINARY SCIENCES | 2022 | 3.7 | 34.9 | 0.14 | 2025-06-25 | 2 | 2 | CERVICAL INTRAEPITHELIAL NEOPLASIA; ANALYTICAL PERFORMANCE; TEST REQUIREMENTS; DNA TEST; GUIDELINES; CANCER; WOMEN; MANAGEMENT; INFECTION; TESTS | Alphapapillomavirus; Cervix Uteri; DNA, Viral; Early Detection of Cancer; Female; Genotype; Humans; Papillomaviridae; Papillomavirus Infections; Sensitivity and Specificity; Uterine Cervical Neoplasms; virus DNA; adult; aged; Article; atypical squamous cells of the cervix; cohort analysis; female; genotyping; high risk population; human; Human papillomavirus type 33; Human papillomavirus type 58; major clinical study; Papillomaviridae; sensitivity and specificity; squamous intraepithelial lesion of the cervix; virus detection; virus load; Alphapapillomavirus; early cancer diagnosis; genetics; genotype; papillomavirus infection; pathology; uterine cervix; uterine cervix tumor | English | 2022 | 2022-07-08 | 10.1371/journal.pone.0267836 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Comparison of Wear of Interim Crowns in Accordance with the Build Angle of Digital Light Processing 3D Printing: A Preliminary In Vivo Study | The aim of this study is to evaluate the wear volume of interim crowns fabricated using digital light processing 3D printing according to the printing angle. A total of five patients undergoing the placement of a single crown on the mandibular molar were included. Interim crowns were fabricated directly in the oral cavity using the conventional method. A digital light processing 3D printer was then used to fabricate crowns with build angles of 0, 45, and 90 degrees. Therefore, four fabricated interim crowns were randomly delivered to the patients, and each was used for one week. Before and after use, the intaglio surfaces of the interim crowns were scanned using a 3D scanner. The volume changes before and after use were measured, and changes in the height of the occlusal surface were evaluated using the root mean square value. Data normality was verified by statistical analysis, and the wear volume in each group was evaluated using a one-way analysis of variance and Tukey's honestly significant difference test (alpha = 0.05). Compared with the RMS values of the conventional method (11.88 +/- 2.69 mu m) and the 3D-printing method at 0 degrees (12.14 +/- 2.38 mu m), the RMS values were significantly high at 90 degrees (16.46 +/- 2.39 mu m) (p < 0.05). Likewise, there was a significant difference in the change in volume between the groups (p = 0.002), with a significantly higher volume change value at 90 degrees (1.74 +/- 0.41 mm(3)) than in the conventional method (0.70 +/- 0.15 mm(3)) (p < 0.05). A printing angle of 90 degrees is not recommended when interim crowns are fabricated using digital light processing 3D printing. | Lee, Hakjun; Son, Keunbada; Lee, Du-Hyeong; Kim, So-Yeun; Lee, Kyu-Bok | Kyungpook Natl Univ, Sch Dent, Dept Prosthodont, Daegu 41940, South Korea; Kyungpook Natl Univ, Adv Dent Device Dev Inst A3DI, Daegu 41940, South Korea | ; SON, Keunbada/AAG-8089-2019; Son, Keunbada/AAG-8089-2019 | 57797775100; 57202916520; 35770948000; 57190972249; 15925571200 | kblee@knu.ac.kr; | BIOENGINEERING-BASEL | BIOENGINEERING-BASEL | 2306-5354 | 9 | 9 | SCIE | ENGINEERING, BIOMEDICAL | 2022 | 4.6 | 34.9 | 0.91 | 2025-06-25 | 11 | 12 | 3D printing; digital light processing; interim crown; in vivo study; wear | 3D printing; digital light processing; in vivo study; interim crown; wear | English | 2022 | 2022-09 | 10.3390/bioengineering9090417 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | COP1 controls salt stress tolerance by modulating sucrose content | The E3 ubiquitin ligase Constitutive Photomorphogenic 1 (COP1) plays evolutionarily conserved and divergent roles. In plants, COP1 regulates a large number of developmental processes including photomorphogenesis, seedling emergence, and gravitropism. Nevertheless, its function in abiotic stress tolerance remains largely unknown. Here, we demonstrate the role of COP1 in salt stress tolerance in Arabidopsis thaliana. In soil, cop1-4 and cop1-6 mutants were more tolerant to high salinity than wild-type (WT) plants during vegetative growth. However, in high salt-containing Murashige and Skoog (MS) medium, cop1-4 and cop1-6 seedlings exhibited significantly impaired growth compared with WT plants. Notably, cop1-4 and cop1-6 seedlings recovered their growth to the WT level upon exogenous sucrose treatment even under high salinity conditions. Compared with WT plants, the sucrose content of cop1-4 mutants was much higher at the vegetative growth stage but similar at the seedling stage. Upon exogenous sucrose supply, root elongation was significantly stimulated in cop1-4 seedlings but only slightly stimulated in WT plants. Thus, no significant difference was observed in root length between the two genotypes. Altogether, our data indicate that cop1 mutants are more tolerant to salt stress than WT plants, and the salt tolerance of cop1 mutants is correlated with their sucrose content. | Kim, Joo Yong; Lee, Seung Ju; Min, Wang Ki; Cha, Seoyeon; Song, Jong Tae; Seo, Hak Soo | Seoul Natl Univ, Res Inst Agr & Life Sci, Dept Agr Forestry & Bioresources, Seoul, South Korea; Kyungpook Natl Univ, Dept Appl Biosci, Daegu, South Korea; Seoul Natl Univ BioMax, Seoul, South Korea | Lee, Jung-Seok/L-6826-2019; KIM, JOO YONG/JBJ-6285-2023 | 58529814100; 57797133900; 57797161600; 57797161700; 57797134000; 26642769200 | seohs@snu.ac.kr; | PLANT SIGNALING & BEHAVIOR | PLANT SIGNAL BEHAV | 1559-2316 | 1559-2324 | 17 | 1 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;PLANT SCIENCES | 2022 | 2.9 | 34.9 | 1.45 | 2025-06-25 | 15 | 13 | Arabidopsis; COP1; cop1-4 mutant; cop1-6 mutant; root growth; salt tolerance; sucrose | ARABIDOPSIS; PHOTOMORPHOGENESIS; LIGHT | Arabidopsis; COP1; cop1-4 mutant; cop1-6 mutant; root growth; salt tolerance; sucrose | Arabidopsis; Arabidopsis Proteins; Gene Expression Regulation, Plant; Light; Salt Stress; Salt Tolerance; Seedlings; Sucrose; Ubiquitin-Protein Ligases; Arabidopsis protein; sucrose; ubiquitin protein ligase; Arabidopsis; gene expression regulation; genetics; light; metabolism; physiology; salt stress; salt tolerance; seedling | English | 2022 | 2022-12-31 | 10.1080/15592324.2022.2096784 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Data integration of National Dose Registry and survey data using multivariate imputation by chained equations | Introduction Data integration is the process of merging information from multiple datasets generated from different sources, which can obtain more information in comparison to to one data source. All diagnostic medical radiation workers were enrolled in National Dose Registry (NDR) from 1996 to 2011, linked with mortality and cancer registry data. (https://kdca.go.kr/) Survey was conducted during 2012-2013 using self-reported questionnaire on occupational radiation practices among diagnostic medical radiation workers. Methods Data integration of NDR and Survey was performed using the multivariate imputation by chained equations (MICE) algorithm. Results The results were compared by sex and type of job because characteristics of target variables for imputation depend on these variables. There was a difference between the observed and pooled mean for the frequency of interventional therapy for nurses due to different type of medical facility distribution between observed and completed data. Concerning the marital status of males and females, and status of pregnancy for females, there was a difference between observed and pooled mean because the distribution of the year of birth was different between the observed and completed data. For lifetime status of smoking, the percentage of smoking experience was higher in the completed data than in the observed data, which could be due to reasons, such as underreporting among females and the distribution difference in the frequency of drinking between the observed and completed data for males. Conclusion Data integration can allow us to obtain survey information of NDR units without additional surveys, saving us time and costs for the survey. | Kim, Ryu Kyung; Kim, Young Min; Lee, Won Jin; Im, Jongho; Lee, Juhee; Bang, Ye Jin; Cha, Eun Shil | Kyungpook Natl Univ, Dept Stat, Daegu, South Korea; Korea Univ, Coll Med, Dept Prevent Med, Seoul, South Korea; Yonsei Univ, Dept Stat & Data Sci, Seoul, South Korea; Yonsei Univ, Dept Appl Stat, Seoul, South Korea; Korea Dis Control & Prevent Agcy, Cheongju, Chuncheongbuk D, South Korea | ; Lee, JooSang/KLC-3096-2024 | 57699350600; 56035273800; 55641918400; 56179626100; 57210897006; 56985424800; 24436753300 | kymmyself@knu.ac.kr; | PLOS ONE | PLOS ONE | 1932-6203 | 17 | 6 | SCIE | MULTIDISCIPLINARY SCIENCES | 2022 | 3.7 | 34.9 | 0.14 | 2025-06-25 | 3 | 2 | MULTIPLE IMPUTATION; RADIOLOGIC TECHNOLOGISTS; SAMPLE | Algorithms; Female; Health Personnel; Humans; Male; Marital Status; Registries; Surveys and Questionnaires; adult; algorithm; article; controlled study; data integration; drinking; female; health survey; human; male; marriage; nurse; pregnancy; smoking; target variable; health care personnel; questionnaire; register | English | 2022 | 2022 | 10.1371/journal.pone.0261534 | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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