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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | ○ | Article | Cross-Species Studies Reveal That Dysregulated Mitochondrial Gene Expression and Electron Transport Complex I Activity Are Crucial for Sarcopenia | The significance of complex I of the electron transport chain (ETC) in the aging process is widely acknowledged; however, its specific impact on the development of sarcopenia in muscle remains poorly understood. This study elucidated the correlation between complex I inhibition and sarcopenia by conducting a comparative analysis of skeletal muscle gene expression in sarcopenia phenotypes from rats, mice, and humans. Our findings reveal a common mechanistic link across species, particularly highlighting the correlation between the suppression of complex I of ETC activity and dysregulated mitochondrial transcription and translation in sarcopenia phenotypes. Additionally, we observed macrophage dysfunction alongside abnormal metabolic processes within skeletal muscle tissues across all species, implicating their pathogenic role in the onset of sarcopenia. These discoveries underscore the importance of understanding the shared mechanisms associated with complex I of ETC in sarcopenia development. The identified correlations provide valuable insights into potential targets for therapeutic interventions aimed at mitigating the impact of sarcopenia, a condition with substantial implications for aging populations. | Lee, Ji-Yoon; Shin, Su-Kyung; Han, Ji-Won; Kwon, Eun-Young; Bae, Heekyong R. | Kyungpook Natl Univ, Dept Food Sci & Nutr, 80 Daehak Ro, Daegu 41566, South Korea; Kyungpook Natl Univ, Ctr Food & Nutr Genom Res, 80 Daehak Ro, Daegu 41566, South Korea; Kyungpook Natl Univ, Ctr Beautiful Aging, 80 Daehak Ro, Daegu 41566, South Korea | 58631087000; 23988149900; 59136406500; 15765422500; 57191253762 | ssk1210@knu.ac.kr;eykwon@knu.ac.kr;baehee@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 25 | 19 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2024 | 4.9 | 22.4 | 0.38 | 2025-05-07 | 0 | 1 | aging; electron transport complex I; macrophage; mitochondria; muscle; skeletal; sarcopenia | SKELETAL-MUSCLE | aging; electron transport complex I; macrophage; mitochondria; muscle; sarcopenia; skeletal | Aging; Animals; Electron Transport Complex I; Female; Gene Expression Regulation; Humans; Male; Mice; Mitochondria; Muscle, Skeletal; Rats; Sarcopenia; gamma interferon; interleukin 6; reduced nicotinamide adenine dinucleotide dehydrogenase (ubiquinone); tumor necrosis factor; reduced nicotinamide adenine dinucleotide dehydrogenase (ubiquinone); animal cell; Article; controlled study; gastrocnemius muscle; gene expression; human; human cell; inflammation; macrophage; macrophage function; metabolome; mitochondrial gene; mouse; muscle tissue; nonhuman; phenotype; rat; respiratory chain; sarcopenia; skeletal muscle; vastus lateralis muscle; aging; animal; female; gene expression regulation; genetics; male; metabolism; mitochondrion; pathology; skeletal muscle | English | 2024 | 2024-10 | 10.3390/ijms251910302 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Development of a Rapid Detection Method for Ethylene Glycol and Glycolic Acid in Feline Samples: A Response to Increasing Antifreeze Poisoning Incidents in Korea | Recently, cases of antifreeze poisoning in companion animals, particularly cats, have surged in the Republic of Korea. Ethylene glycol (EG), the toxic primary component of antifreeze, is metabolized into glycolic acid (GA), leading to severe metabolic acidosis, acute kidney injury, and death. Traditional detection methods, although effective, are often time-consuming owing to complex sample preparation. This study involved a novel analytical method utilizing GC-MS for EG and LC-MS/MS for GA detection, which streamlined the detection process by eliminating the need for derivatization. The method was validated for accuracy and reliability, enabling the rapid and precise identification of EG and GA in biological samples. This study also included the successful application of this method in a case where initial exposure to antifreeze was not apparent, which highlighted the effectiveness of this method in diagnosing poisoning even in cases where clinical history is unclear. The development of this rapid diagnostic approach addresses the urgent need for the efficient detection of antifreeze poisoning, improving animal welfare and supporting forensic investigations. | Chae, Hyunyoung; Byun, Jae Won; Shin, Go-Eun; Lee, Kyung Hyun; Kim, Ah-Young; Ku, Bok-Kyung; Hossain, Md Akil; Kim, Tae-Wan; Kang, Jeongwoo | Minist Agr Food & Rural Affairs, Anim & Plant Quarantine Agcy APQA, Anim Dis Diag Div, 177 Hyeoksin 8 Ro, Gimcheon Si 39660, South Korea; Kyungpook Natl Univ, Coll Vet Med, Lab Vet Physiol, Daegu 41566, South Korea; Univ Illinois, Inst TB Res, Coll Pharm, 833 S Wood St MC964, Chicago, IL 60612 USA | ; Hossain, Akil/AAY-7229-2020 | 58175968000; 35075815400; 57226653598; 35183367100; 7402063210; 9745131200; 55754133800; 57202769925; 57212201397 | iichy33ii@korea.kr;jaewon8911@korea.kr;tlsrhdms924@korea.kr;mylovehyun@korea.kr;mochsha@korea.kr;kubk@korea.kr;mdakil@uic.edu;twkim@knu.ac.kr;hijach@korea.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 25 | 20 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2024 | 4.9 | 22.4 | 0 | 2025-05-07 | 0 | 0 | antifreeze poisoning; ethylene glycol; glycolic acid; rapid diagnostic method | HUMAN SERUM; DIAGNOSIS; URINE; DOGS | antifreeze poisoning; ethylene glycol; glycolic acid; rapid diagnostic method | Animals; Cats; Chromatography, Liquid; Ethylene Glycol; Gas Chromatography-Mass Spectrometry; Glycolates; Reproducibility of Results; Republic of Korea; Tandem Mass Spectrometry; eosin; ethylene glycol; glycolic acid; hematoxylin; ethylene glycol; glycolic acid; glycolic acid derivative; analytic method; animal experiment; Article; blood sampling; cat; criminalistics; derivatization; intoxication; Korea; liquid chromatography-mass spectrometry; mass fragmentography; mass spectrometry; nonhuman; polymerase chain reaction; animal; cat; liquid chromatography; procedures; reproducibility; South Korea; tandem mass spectrometry | English | 2024 | 2024-10 | 10.3390/ijms252011030 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | ECG data analysis to determine ST-segment elevation myocardial infarction and infarction territory type: an integrative approach of artificial intelligence and clinical guidelines | Introduction Acute coronary syndrome (ACS) is one of the leading causes of death from cardiovascular diseases worldwide, with ST-segment elevation myocardial infarction (STEMI) representing a severe form of ACS that exhibits high prevalence and mortality rates. This study proposes a new method for accurately diagnosing STEMI and categorizing the infarction area in detail, based on 12-lead electrocardiogram (ECG) data using a deep learning-based artificial intelligence (AI) algorithm.Methods Utilizing an ECG database consisting of 888 myocardial infarction (MI) patients, this study enhanced the generalization ability of the AI model through five-fold cross-validation. The developed ST-segment elevation (STE) detector accurately identified STE across all 12 leads, which is a crucial indicator for the clinical ECG diagnosis of STEMI. This detector was employed in the AI model to differentiate between STEMI and non-ST-segment elevation myocardial infarction (NSTEMI).Results In the process of distinguishing between STEMI and NSTEMI, the average area under the receiver operating characteristic curve (AUROC) was 0.939, and the area under the precision-recall curve (AUPRC) was 0.977, demonstrating significant results. Furthermore, this detector exhibited the ability to accurately differentiate between various infarction territories in the ECG, including anterior myocardial infarction (AMI), inferior myocardial infarction (IMI), lateral myocardial infarction (LMI), and suspected left main disease.Discussion These results suggest that integrating clinical domains into AI technology for ECG diagnosis can play a crucial role in the rapid treatment and improved prognosis of STEMI patients. This study provides an innovative approach for the diagnosis of cardiovascular diseases and contributes to enhancing the practical applicability of AI-based diagnostic tools in clinical settings. | Kim, Jongkwang; Shon, Byungeun; Kim, Sangwook; Cho, Jungrae; Seo, Jung-Ju; Jang, Se Yong; Jeong, Sungmoon | Kyungpook Natl Univ, Sch Med, Dept Med Informat, Daegu 700422, South Korea; Kyungpook Natl Univ Hosp, Res Ctr AI Med, Daegu, South Korea; Kyungpook Natl Univ Hosp, Biomed Res Inst, Daegu, South Korea; Kyungpook Natl Univ, Sch Med, Dept Internal Med, Daegu, South Korea; Kyungpook Natl Univ, Dept Internal Med, Div Cardiol, Chilgok Hosp, Daegu 41404, South Korea | 59226639400; 58635954000; 59377738500; 57205507149; 59377583700; 57207977889; 23100090400 | seyongjang@knu.ac.kr;jeongsm00@gmail.com; | FRONTIERS IN PHYSIOLOGY | FRONT PHYSIOL | 1664-042X | 15 | SCIE | PHYSIOLOGY | 2024 | 3.4 | 22.4 | 0 | 2025-05-07 | 0 | 1 | ST-segment elevation detection; deep learning-based artificial intelligence; ST-segment elevation myocardial infarction; 12-lead electrocardiogram; infarction territory | PERCUTANEOUS CORONARY INTERVENTION; CARDIOGENIC-SHOCK; MORTALITY | 12-lead electrocardiogram; deep learning-based artificial intelligence; infarction territory; ST-segment elevation detection; ST-segment elevation myocardial infarction | acute heart infarction; Article; artificial intelligence; artificial neural network; cardiac muscle cell; clinical practice guideline; coronary artery disease; cross validation; diagnostic accuracy; electrocardiogram; electrocardiography; human; inferior myocardial infarction; machine learning; mortality rate; non ST segment elevation myocardial infarction; prevalence; QRS complex; receiver operating characteristic; ST segment elevation; ST segment elevation myocardial infarction; support vector machine; validation process | English | 2024 | 2024-10-07 | 10.3389/fphys.2024.1462847 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Editorial Material | Editorial for the Special Issue "Recent Advances in Nanomaterials Science" | Lee, Gang Ho | Kyungpook Natl Univ, Coll Nat Sci, Dept Chem, Daegu 41566, South Korea | 7404851841 | ghlee@mail.knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 25 | 10 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2024 | 4.9 | 22.4 | 0 | 2025-05-07 | 0 | 0 | Biosensing Techniques; Humans; Nanomedicine; Nanoparticles; Nanostructures; Nanotechnology; Solar Energy; heavy metal; metal nanoparticle; nanomaterial; nanoparticle; polymer nanoparticle; water; catalysis; Editorial; magnetic separation; nanomedicine; particle size; physical chemistry; chemistry; genetic procedures; human; nanotechnology; procedures; solar energy | English | 2024 | 2024-05 | 10.3390/ijms25105541 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||||
| ○ | ○ | Article | Effects of Rumen-Protected L-Tryptophan Supplementation on Productivity, Physiological Indicators, Blood Profiles, and Heat Shock Protein Gene Expression in Lactating Holstein Cows under Heat Stress Conditions | In this study, we examined the effects of rumen-protected L-tryptophan supplementation on the productivity and physiological metabolic indicators in lactating Holstein cows under heat stress conditions. The study involved eight early lactating Holstein cows (days in milk = 40 +/- 9 days; milk yield 30 +/- 1.5 kg/day; parity 1.09 +/- 0.05, p < 0.05), four cows per experiment, with environmentally controlled chambers. In each experiment, two distinct heat stress conditions were created: a low-temperature and low-humidity (LTLH) condition at 25 degrees C with 35-50% humidity and a high-temperature and high-humidity (HTHH) condition at 31 degrees C with 80-95% humidity. During the adaptation phase, the cows were subjected to LTLH and HTHH conditions for 3 days. This was followed by a 4-day heat stress phase and then by a 7-day phase of heat stress, which were complemented by supplementation with rumen-protected L-tryptophan (ACT). The findings revealed that supplementation with ACT increased dry matter intake as well as milk yield and protein and decreased water intake, heart rate, and rectal temperature in the HTHH group (p < 0.05). For plateletcrit (PCT, p = 0.0600), the eosinophil percentage (EOS, p = 0.0880) showed a tendency to be lower, while the monocyte (MONO) and large unstained cells (LUC) amounts were increased in both groups (p < 0.05). Albumin and glucose levels were lower in the HTHH group (p < 0.05). The gene expressions of heat shock proteins 70 and 90 in the peripheral blood mononuclear cells were higher in the ACT group (HTHH, p < 0.05). These results suggest that ACT supplementation improved productivity, physiological indicators, blood characteristics, and gene expression in the peripheral blood mononuclear cells of early lactating Holstein cows under heat-stress conditions. In particular, ACT supplementation objectively relieved stress in these animals, suggesting that L-tryptophan has potential as a viable solution for combating heat-stress-induced effects on the cattle in dairy farming. | Jo, Jang-Hoon; Jalil, Ghassemi Nejad; Kim, Won-Seob; Moon, Jun-Ok; Lee, Sung-Dae; Kwon, Chan-Ho; Lee, Hong-Gu | Konkuk Univ, Sanghuh Coll Life Sci, Dept Anim Sci & Technol, Seoul 05029, South Korea; Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA; CJ CheilJedang, Inst Integrated Technol, Suwon 16495, South Korea; Rural Dev Adm, Natl Inst Anim Sci, Anim Nutr & Physiol Team, Wonju 55365, South Korea; Kyungpook Natl Univ, Dept Anim Sci, Sangju 37224, South Korea | Nejad, Jalil/V-4645-2019; Kwon, Chan Ho/HGE-7846-2022 | 57217207224; 58119163200; 57192382284; 57212004119; 57192503342; 57201618361; 35074370700 | godandthegod@naver.com;jalilgh@konkuk.ac.kr;kws9285@hanmail.net;junok.moon@cj.net;leesd@korea.kr;chkwon@knu.ac.kr;hglee66@konkuk.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 25 | 2 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2024 | 4.9 | 22.4 | 1.25 | 2025-04-16 | 3 | 3 | heat stress; rumen protected L-tryptophan; lactating Holstein cow; welfare | DAIRY-COWS; MILK-YIELD; DIETARY TRYPTOPHAN; INTESTINAL INTEGRITY; SEROTONIN LEVELS; GHRELIN LEVELS; SHORT-TERM; METABOLISM; TEMPERATURE; PERFORMANCE | heat stress; lactating Holstein cow; rumen protected L-tryptophan; welfare | Animals; Cattle; Diet; Dietary Supplements; Female; Gene Expression; Heat-Shock Proteins; Heat-Shock Response; Hot Temperature; Lactation; Leukocytes, Mononuclear; Milk; Pregnancy; Rumen; Tryptophan; albumin; glucose; heat shock protein; heat shock protein 70; heat shock protein 90; milk protein; rumen protected tryptophan; tryptophan; unclassified drug; heat shock protein; tryptophan; albumin level; animal cell; animal experiment; Article; chemical composition; controlled study; diet supplementation; dry matter intake; eosinophil percentage; female; fluid intake; food intake; gene expression; glucose level; hair follicle; heart rate; heat stress; hematological parameters; high temperature; Holstein cattle; humidity; lactation; low temperature; metabolic regulation; milk yield; monocyte; nonhuman; peripheral blood mononuclear cell; physiological process; plateletcrit; rectal temperature; animal; bovine; diet; dietary supplement; gene expression; genetics; heat shock response; metabolism; milk; mononuclear cell; physiology; pregnancy; rumen; veterinary medicine | English | 2024 | 2024-01 | 10.3390/ijms25021217 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Effects of Siegesbeckia herba extract against particulate matter 10 (PM10) in skin barrier-disrupted mouse models | Objectives: Skin barrier disruption is a significant problem of the older population in an aging society. It is characterized by increased transepidermal water loss and decreased skin water content, and particulate matter (PM) is a social issue that can contribute to the exacerbation of skin inflammation. Thus, addressing this problem is urgent. Methods: Skin barrier-disrupted mouse models were induced by two methods using acetone application or tape-stripping. This study investigated the antioxidative and anti-inflammatory properties of the Siegesbeckia herba extract (SHE) on PM-induced changes in skin barrier-disrupted mouse models. To examine changes in skin water content, inflammatory cytokines, and keratinocyte differentiation markers, mouse models were treated with vehicle 100 mu L, PM10 100 mu L (100 mu g/mL), SHE 100 mu L, or PM10 100 mu L (100 mu g/mL) plus SHE 100 mu L. Results: SHE preserved skin hydration in the skin barrier-disrupted mouse models regardless of the presence of PM10. SHE also inhibited the upregulation of inflammatory cytokines such as interleukin (IL)-1 beta, IL-4, IL-6, IL-8, and tumor necrosis factor-alpha and normalized the downregulation of keratinocyte differentiation markers against PM10 in skin barrier-disrupted mouse models. Conclusions: This study elucidated the therapeutic effects of SHE against PM10 in skin barrier-disrupted mouse models. | Park, Kyung Duck; Kwack, Mi Hee; Yoon, Hyo Jin; Lee, Weon Ju | Kyungpook Natl Univ, Sch Med, Dept Dermatol, Daegu, South Korea; Kyungpook Natl Univ, Sch Med, Dept Immunol, Daegu, South Korea; Kyungpook Natl Univ Hosp, Dept Dermatol, 130 Dongdeok Ro, Daegu, South Korea | 55767995700; 6507685557; 58896787300; 24474659000 | weonju@knu.ac.kr; | SKIN RESEARCH AND TECHNOLOGY | SKIN RES TECHNOL | 0909-752X | 1600-0846 | 30 | 3 | SCIE | DERMATOLOGY | 2024 | 3.2 | 22.4 | 3.74 | 2025-05-07 | 4 | 4 | Particulate matter; Siegesbeckia herba extract; Skin barrier disruption; Xerosis | DISORDERS; REPAIR | Particulate matter; Siegesbeckia herba extract; Skin barrier disruption; Xerosis | Animals; Antigens, Differentiation; Cytokines; Mice; Particulate Matter; Sigesbeckia; Water; Cell death; Extraction; Particles (particulate matter); acetone; antiinflammatory agent; antioxidant; interleukin 1beta; interleukin 4; interleukin 6; interleukin 8; plant extract; Siegesbeckia herba extract; tumor necrosis factor; unclassified drug; cytokine; differentiation antigen; water; Differentiation markers; Inflammatory cytokines; Keratinocyte differentiation; Mice models; Particulate Matter; Particulate matter 10; Siegesbeckium herba extract; Skin barrier disruption; Skin waters; Xerosis; aging; animal experiment; animal model; animal tissue; antiinflammatory activity; antioxidant activity; Article; down regulation; hydration; keratinocyte; medical procedures; mouse; mouse model; nonhuman; particulate matter; particulate matter 10; real time polymerase chain reaction; Siegesbeckia; Siegesbeckia herba; skin; skin barrier disruption; skin disease; skin water loss; tape stripping; upregulation; water content; xerosis; animal; particulate matter; Acetone | English | 2024 | 2024-03 | 10.1111/srt.13615 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Efficacy of Integrated Risk Score Using Omics-Based Biomarkers for the Prediction of Acute Rejection in Kidney Transplantation: A Randomized Prospective Pilot Study | Acute rejection (AR) is critical for long-term graft survival in kidney transplant recipients (KTRs). This study aimed to evaluate the efficacy of the integrated risk score of omics-based biomarkers in predicting AR in KTRs. This prospective, randomized, controlled, multicenter, pilot study enrolled 40 patients who recently underwent high-immunologic-risk kidney transplantation (KT). Five omics biomarkers were measured, namely, blood mRNA (three-gene signature), urinary exosomal miRNA (three-gene signature), urinary mRNA (six-gene signature), and two urinary exosomal proteins (hemopexin and tetraspanin-1) at 2 weeks and every 4 weeks after KT for 1 year. An integrated risk score was generated by summing each biomarker up. The biomarker group was informed about the integrated risk scores and used to adjust immunosuppression, but not the control group. The outcomes were graft function and frequency of graft biopsy. Sixteen patients in the biomarker group and nineteen in the control group completed the study. The mean estimated glomerular filtration rate after KT did not differ between the groups. Graft biopsy was performed in two patients (12.5%) and nine (47.4%) in the biomarker and control groups, respectively, with the proportion being significantly lower in the biomarker group (p = 0.027). One patient (6.3%) in the biomarker group and two (10.5%) in the control group were diagnosed with AR, and the AR incidence did not differ between the groups. The tacrolimus trough level was significantly lower in the biomarker group than in the control group at 1 year after KT (p = 0.006). Integrated omics biomarker monitoring may help prevent unnecessary or high-complication-risk biopsy and enables tailored immunosuppression by predicting the risk of AR in KTRs. | Lim, Jeong-Hoon; Chung, Byung Ha; Lee, Sang-Ho; Lee, Jong Soo; Kim, Yeong Hoon; Han, Man-Hoon; Jung, Hee-Yeon; Choi, Ji-Young; Cho, Jang-Hee; Park, Sun-Hee; Kim, Yong-Lim; Kim, Chan-Duck | Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Div Nephrol,Dept Internal Med, Daegu 41944, South Korea; Catholic Univ Korea, Seoul St Marys Hosp, Coll Med, Dept Internal Med,Div Nephrol, Seoul 06591, South Korea; Kyung Hee Univ, Coll Med, Dept Internal Med, Div Nephrol, Seoul 02447, South Korea; Inje Univ, Coll Med, Dept Internal Med, Div Nephrol, Pusan 44033, South Korea; Inje Univ, Busan Paik Hosp, Coll Med, Dept Internal Med,Div Nephrol, Busan 47392, South Korea; Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Dept Pathol, Daegu 41944, South Korea | Lim, Jeong-Hoon/ABE-6003-2020; Kim, Tae-Hee/AAN-9079-2021; Park, Sun-Hee/LMN-0033-2024; Jung, Hee-Yeon/AFB-8578-2022; Kim, Yong-Lim/AGK-3172-2022; Cho, Jang-hee/ABD-3534-2020 | 55360244300; 57201863822; 55890136000; 57201264463; 7410196419; 57194067936; 57196396467; 7501393222; 7403536291; 7501831741; 55633533600; 8558530700 | jh-lim@knu.ac.kr;chungbh@catholic.ac.kr;lshkidney@khu.ac.kr;yeonghnl@inje.ac.kr;mhhan1@knu.ac.kr;hy-jung@knu.ac.kr;jyss1002@hanmail.net;jh-cho@knu.ac.kr;sh-park@knu.ac.kr;ylkim@knu.ac.kr;drcdkim@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 25 | 10 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2024 | 4.9 | 22.4 | 1.51 | 2025-05-07 | 4 | 4 | biomarker; graft rejection; kidney transplantation; omics | RECIPIENTS; DIALYSIS | biomarker; graft rejection; kidney transplantation; omics | Adult; Biomarkers; Female; Graft Rejection; Graft Survival; Humans; Kidney Transplantation; Male; MicroRNAs; Middle Aged; Pilot Projects; Prospective Studies; Risk Assessment; Risk Factors; basiliximab; biological marker; calcineurin inhibitor; complement component C1q; creatinine; CXCL9 chemokine; gamma interferon inducible protein 10; hemopexin; hepatitis A virus cellular receptor 2; immunosuppressive agent; messenger RNA; microRNA; microRNA 21; microRNA 31; mycophenolic acid; tacrolimus; tetraspanin 1; thymocyte antibody; unclassified drug; biological marker; adult; Article; BK virus nephropathy; capillaritis; clinical article; controlled study; creatinine blood level; estimated glomerular filtration rate; exosome; female; glomerulitis; human; immunohistochemistry; immunosuppressive treatment; kidney graft rejection; kidney transplantation; male; microvasculitis; middle aged; multicenter study; nephrotoxicity; omics; pilot study; prediction; prospective study; proteinuria; randomized controlled trial; adverse event; blood; clinical trial; diagnosis; genetics; graft rejection; graft survival; risk assessment; risk factor; urine | English | 2024 | 2024-05 | 10.3390/ijms25105139 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Efficient Assessment of Tumor Vascular Shutdown by Photodynamic Therapy on Orthotopic Pancreatic Cancer Using High-Speed Wide-Field Waterproof Galvanometer Scanner Photoacoustic Microscopy | To identify the vascular alteration by photodynamic therapy (PDT), the utilization of high-resolution, high-speed, and wide-field photoacoustic microscopy (PAM) has gained enormous interest. The rapid changes in vasculature during PDT treatment and monitoring of tumor tissue activation in the orthotopic pancreatic cancer model have received limited attention in previous studies. Here, a fully two-axes waterproof galvanometer scanner-based photoacoustic microscopy (WGS-PAM) system was developed for in vivo monitoring of dynamic variations in micro blood vessels due to PDT in an orthotopic pancreatic cancer mouse model. The photosensitizer (PS), Chlorin e6 (Ce6), was utilized to activate antitumor reactions in response to the irradiation of a 660 nm light source. Microvasculatures of angiogenesis tissue were visualized on a 40 mm2 area using the WGS-PAM system at 30 min intervals for 3 h after the PDT treatment. The decline in vascular intensity was observed at 24.5% along with a 32.4% reduction of the vascular density at 3 h post-PDT by the analysis of PAM images. The anti-vascularization effect was also identified with fluorescent imaging. Moreover, Ce6-PDT increased apoptotic and necrotic markers while decreasing vascular endothelial growth factor (VEGF) expression in MIA PaCa-2 and BxPC-3 pancreatic cancer cell lines. The approach of the WGS-PAM system shows the potential to investigate PDT effects on the mechanism of angiographic dynamics with high-resolution wide-field imaging modalities. | Lee, Jaeyul; Han, Sangyeob; Magar, Til Bahadur Thapa; Gurung, Pallavi; Lee, Junsoo; Seong, Daewoon; Park, Sungjo; Kim, Yong-Wan; Jeon, Mansik; Kim, Jeehyun | Kyungpook Natl Univ, Coll IT Engn, Sch Elect & Elect Engn, Daegu 41566, South Korea; Kyungpook Natl Univ, KNU Inst Nanophoton Applicat KINPA, Sch Appl Chem Engn, Dept Chem Engn,Organ Nanoelect Lab, Daegu 41566, South Korea; Kyungpook Natl Univ, Inst Biomed Engn Res, Daegu 41566, South Korea; Dongsung Bio Pharmaceut Co Ltd, Dongsung Canc Ctr, Daegu 41061, South Korea; Kyungpook Natl Univ, Inst Adv Convergence Technol, Laser Applicat Ctr, Daegu 41061, South Korea; Harvard Med Sch, Massachusetts Gen Hosp, Div Pulm & Crit Care Med, Boston, MA 02114 USA | ; Thapa Magar, Til Bahadur/S-4529-2016; Han, Sangyeob/W-3206-2018; Lee, Junsoo/IUO-9627-2023; Lee, Jung Bok/HHZ-3200-2022 | 57188689420; 57193695305; 57189299456; 57119548200; 57209986073; 57212512353; 7501826338; 57223792129; 24171094000; 7601373350 | jlee350@mgh.harvard.edu;syhan850224@knu.ac.kr;til09@ds-pharm.co.kr;gp20@ds-pharm.co.kr;junsoo.lee@uci.edu;smc7095@knu.ac.kr;sungjo@iact.or.kr;kyw17@ds-pharm.co.kr;msjeon@knu.ac.kr;jeehk@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 25 | 6 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2024 | 4.9 | 22.4 | 0.76 | 2025-05-07 | 1 | 2 | photoacoustic microscopy; waterproof galvanometer scanner; photodynamic therapy; pancreatic cancer; chlorin e6 | COMBRETASTATIN A-4 PHOSPHATE; CHLORIN E6; HIGH-RESOLUTION; CELLS; CHEMOTHERAPY; RESECTION; DELIVERY; MICROENVIRONMENT; PHOTOSENSITIZERS; NANOPARTICLES | chlorin e6; pancreatic cancer; photoacoustic microscopy; photodynamic therapy; waterproof galvanometer scanner | Animals; Cell Line, Tumor; Chlorophyllides; Mice; Microscopy; Pancreatic Neoplasms; Photochemotherapy; Photosensitizing Agents; Porphyrins; Vascular Endothelial Growth Factor A; antineoplastic agent; baculoviral IAP repeat containing protein 5; caspase 3; caspase 7; caspase 9; chlorin e6; deoxyhemoglobin; fluorescent dye; luciferin; nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase 1; oxyhemoglobin; phonozen; photosensitizing agent; povidone; protein Bax; protein bcl 2; unclassified drug; vasculotropin; chlorophyllide; photosensitizing agent; porphyrin; vasculotropin A; animal cell; animal experiment; animal model; animal tissue; apoptosis; Article; bioluminescence; blood vessel density; BxPC-3 cell line; controlled study; experimental pancreatic cancer; female; fluorescence imaging; fluorescence intensity; high speed wide field waterproof galvanometer scanner based photoacoustic microscopy; histopathology; human; human cell; image processing; in vitro study; MIA PaCa-2 cell line; microvasculature; mouse; necroptosis; nonhuman; pancreas tissue; photoacoustic microscopy; photodynamic therapy; protein expression; protein phosphorylation; qualitative analysis; tumor vascularization; Western blotting; animal; diagnostic imaging; microscopy; pancreas tumor; pathology; photochemotherapy; procedures; tumor cell line | English | 2024 | 2024-03 | 10.3390/ijms25063457 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Enhanced Antitumor Efficacy of Oncolytic Vaccinia Virus Therapy Through Keratin-Mediated Delivery in Triple-Negative Breast Cancer | Triple-negative breast cancer (TNBC) represents an aggressive subtype characterized by high rates of recurrence and metastasis, necessitating the exploration of alternative treatment strategies. Oncolytic vaccinia virus (OVV) therapy has emerged as a promising approach, selectively infecting and destroying tumor cells. However, its efficacy is often hampered by inadequate viral distribution within the tumor microenvironment. Here, we investigate the potential of keratin (KTN) as a carrier for OVV delivery to enhance viral distribution and antitumor efficacy. In vitro assays revealed that KTN significantly improves OVV stability, leading to increased tumor cell apoptosis and necrosis. Furthermore, KTN effectively inhibits cancer cell migration by suppressing the epithelial-mesenchymal transition (EMT) process and downregulating metastasis-related proteins. These findings are corroborated in a syngeneic TNBC mouse model, where KTN-mediated OVV delivery enhances cytotoxic T cell-mediated antitumor immune responses without compromising the anti-angiogenic effects of the virus. Notably, KTN alone exhibits antitumor effects by suppressing tumor growth and metastasis, underscoring its potential as a standalone therapeutic agent. In conclusion, our study underscores the promise of KTN-mediated OVV delivery as a promising therapeutic strategy for TNBC. By improving viral distribution, suppressing EMT, and enhancing antitumor immunity, this approach holds significant potential for enhancing patient outcomes in TNBC treatment. Further investigation is warranted to explore the broader utility of KTN in various cancer therapy approaches. | Kim, Hyo-Sung; Youn, Yun Hee; Kim, Han-Jun; Koo, Young-Hyun; Lee, Junho; Kwon, Il Keun; Do, Sun Hee | Konkuk Univ, Coll Vet Med, Dept Vet Clin Pathol, 120 Neungdong Ro, Seoul 05029, South Korea; Kyung Hee Univ, Sch Dent, Dept Dent Mat, 26 Kyungheedae Ro, Seoul 02477, South Korea; Korea Univ, Coll Pharm, 2511 Sejong Ro, Sejong 30019, South Korea; Kyungpook Natl Univ, Coll Vet Med, Dept Vet Surg, Daegu 41566, South Korea; Kyung Hee Univ, Med Sci Res Inst, Med Device Res Ctr, Med Ctr, 23 Kyung Hee Dae Ro, Seoul 02447, South Korea | ; YOUN, YUNHEE/E-2148-2016; Kim, Hanjun/AAJ-7528-2021; Kim, Hyosung/LNQ-1108-2024 | 57206693038; 57198345536; 55957698800; 59402967200; 57219032336; 26643276900; 57209079865 | kwoni@khu.ac.kr;shdo@konkuk.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 25 | 21 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2024 | 4.9 | 22.4 | 0 | 2025-05-07 | 2 | 0 | triple-negative breast cancer; keratin; oncolytic vaccinia virus; delivery system | POXVIRUS; VASCULATURE; COMBINATION; EXTRACTION; SPREAD; STAT3 | delivery system; keratin; oncolytic vaccinia virus; triple-negative breast cancer | Animals; Apoptosis; Cell Line, Tumor; Cell Movement; Epithelial-Mesenchymal Transition; Female; Humans; Keratins; Mice; Mice, Inbred BALB C; Oncolytic Virotherapy; Oncolytic Viruses; Triple Negative Breast Neoplasms; Vaccinia virus; Xenograft Model Antitumor Assays; caspase 3; CD3 antigen; CD56 antigen; endoglin; gelatinase B; keratin; oncolytic Vaccinia virus; platelet endothelial cell adhesion molecule 1; STAT3 protein; uvomorulin; vasculotropin; vimentin; oncolytic virus; 4T1 cell line; animal cell; animal experiment; animal model; animal tissue; antiangiogenic activity; antineoplastic activity; antiproliferative activity; apoptosis; Article; cell viability; comparative study; controlled study; cytotoxic T lymphocyte; down regulation; drug delivery system; epithelial mesenchymal transition; female; human; human cell; immunocompetent cell; immunomodulation; in vitro study; in vivo study; migration inhibition; mouse; natural killer T cell; nonhuman; peripheral blood mononuclear cell; triple negative breast cancer; tumor associated leukocyte; tumor invasion; tumor microenvironment; virus replication; animal; Bagg albino mouse; cell motion; drug screening; genetics; metabolism; oncolytic virotherapy; pathology; physiology; procedures; therapy; triple negative breast cancer; tumor cell line; Vaccinia virus | English | 2024 | 2024-11 | 10.3390/ijms252111470 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Evaluation of Teneligliptin and Retagliptin on the Clearance of Melanosome by Melanophagy in B16F1 Cells | A specialized membrane-bound organelle, named the melanosome, is central to the storage and transport of melanin as well as melanin synthesis in melanocytes. Although previous studies have linked melanosomal degradation to autophagy, the precise mechanisms remain elusive. Autophagy, a complex catabolic process involving autophagosomes and lysosomes, plays a vital role in cellular constituent degradation. In this study, the role of autophagy in melanosomal degradation was explored, employing a cell-based screening system designed to unveil key pathway regulators. We identified specific dipeptidyl peptidase-4 inhibitors, such as teneligliptin hydrobromide and retagliptin phosphate, as novel agents inducing melanophagy through a comprehensive screening of a ubiquitination-related chemical library. We found that treatment with teneligliptin hydrobromide or retagliptin phosphate not only diminishes melanin content elevated by alpha-melanocyte-stimulating hormone (alpha-MSH) but also triggers autophagy activation within B16F1 cells. In addition, the targeted inhibition of unc-51-like kinase (ULK1) significantly attenuated both the anti-pigmentation effects and autophagy induced by teneligliptin hydrobromide and retagliptin phosphate in alpha-MSH-treated cells. Collectively, our data demonstrate a new frontier in understanding melanosomal degradation, identifying teneligliptin hydrobromide and retagliptin phosphate as promising inducers of melanophagy via autophagy activation. This study contributes essential insights into cellular degradation mechanisms and offers potential therapeutic avenues in the regulation of pigmentation. | Kim, Seong Hyun; Bae, Ji-Eun; Park, Na Yeon; Kim, Joon Bum; Kim, Yong Hwan; Kim, So Hyun; Oh, Gyeong Seok; Wang, Hee Won; Chang, Jeong Ho; Cho, Dong-Hyung | Kyungpook Natl Univ, Sch Life Sci, BK21 FOUR KNU Creat BioRes Grp, Daegu 41566, South Korea; Kyungpook Natl Univ, Inst Basic Sci, KNU LAMP Res Ctr, Daegu 41566, South Korea; Kyungpook Natl Univ, Dept Biol Educ, Daegu 41566, South Korea; Kyungpook Natl Univ, Organelle Inst, Daegu 41566, South Korea | Choi, Hye Rin/JDV-9065-2023; Kim, Joon/ADP-8066-2022 | 57253406700; 57190605352; 57190609826; 57190611030; 57204676401; 57253406800; 57219084322; 59004132300; 57203598905; 35093684400 | kgj010@naver.com;loveg730@naver.com;yeonie5613@gmail.com;kss3213@naver.com;yoo035913@gmail.com;ks90608@naver.com;dhrudtjr2468@naver.com;dhcho@knu.ac.kr; | COSMETICS | COSMETICS-BASEL | 2079-9284 | 11 | 2 | ESCI | BIOCHEMISTRY & MOLECULAR BIOLOGY;DERMATOLOGY | 2024 | 3.2 | 22.4 | 0 | 2025-05-07 | 0 | 0 | dipeptidyl peptisase-4 inhibitors; teneligliptin hydrobromide; retagliptin phosphate; melanophagy; melanosome | DIPEPTIDYL-PEPTIDASE-IV; SELECTIVE AUTOPHAGY; MELANOGENESIS; TFEB; PIGMENTATION; INFLAMMATION; DEGRADATION; INHIBITION; SENESCENCE; SKIN | dipeptidyl peptisase-4 inhibitors; melanophagy; melanosome; retagliptin phosphate; teneligliptin hydrobromide | English | 2024 | 2024-04 | 10.3390/cosmetics11020035 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Evolution and Competitive Struggles of Lactiplantibacillus plantarum under Different Oxygen Contents | Lactiplantibacillus (Lb.) plantarum is known as a benign bacterium found in various habitats, including the intestines of animals and fermented foods. Since animal intestines lack oxygen, while fermented foods provide a limited or more oxygen environment, this study aimed to investigate whether there were genetic differences in the growth of Lb. plantarum under aerobic vs. anaerobic conditions. Genomic analysis of Lb. plantarum obtained from five sources-animals, dairy products, fermented meat, fermented vegetables, and humans-was conducted. The analysis included not only an examination of oxygen-utilizing genes but also a comparative pan-genomic analysis to investigate evolutionary relationships between genomes. The ancestral gene analysis of the evolutionary pathway classified Lb. plantarum into groups A and B, with group A further subdivided into A1 and A2. It was confirmed that group A1 does not possess the narGHIJ operon, which is necessary for energy production under limited oxygen conditions. Additionally, it was found that group A1 has experienced more gene acquisition and loss compared to groups A2 and B. Despite an initial assumption that there would be genetic distinctions based on the origin (aerobic or anaerobic conditions), it was observed that such differentiation could not be attributed to the origin. However, the evolutionary process indicated that the loss of genes related to nitrate metabolism was essential in anaerobic or limited oxygen conditions, contrary to the initial hypothesis. | Heo, Sojeong; Jung, Eun Jin; Park, Mi-Kyung; Sung, Moon-Hee; Jeong, Do-Won | Dongduk Womens Univ, Dept Food & Nutr, Seoul 02748, South Korea; Minist Food & Drug Safety, Cheongju 28159, South Korea; Kyungpook Natl Univ, Food & Bioind Res Inst, Sch Food Sci & Biotechnol, Daegu 41566, South Korea; KookminBio Corp, Seoul 02826, South Korea | Park, Mi-Kyung/J-9643-2017 | 57191537574; 59306700700; 7404491155; 7202291188; 15623117800 | hsjeong325v@gmail.com;alwalj@korea.kr;parkmik@knu.ac.kr;smoonhee@kookmin.ac.kr;jeongdw@dongduk.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 25 | 16 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2024 | 4.9 | 22.4 | 1.13 | 2025-05-07 | 3 | 3 | Lactiplantibacillus plantarum; pan-genome; core-genome; nitrate metabolism | COLI NITRATE REDUCTASE; LACTIC-ACID BACTERIA; LACTOBACILLUS-PLANTARUM; LACTATE UTILIZATION; NARGHI | core-genome; Lactiplantibacillus plantarum; nitrate metabolism; pan-genome | Anaerobiosis; Animals; Evolution, Molecular; Genome, Bacterial; Humans; Lactobacillus plantarum; Oxygen; Phylogeny; nitrate reductase; oxygen; reactive oxygen metabolite; oxygen; Article; controlled study; core genome; dairy product; energy yield; evolution; fermented product; genetic analysis; genetic difference; genomics; habitat; human; Lactiplantibacillus plantarum; Lactobacillus plantarum; nitrate metabolism; nonhuman; operon; pangenome; respiratory system; vegetable; anaerobic growth; animal; bacterial genome; genetics; Lactobacillus plantarum; metabolism; molecular evolution; phylogeny | English | 2024 | 2024-08 | 10.3390/ijms25168861 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Review | Exploring Plant-Bacterial Symbiosis for Eco-Friendly Agriculture and Enhanced Resilience | This review explores the intricate relationship between plants and bacterial endophytes, revealing their multifaceted roles in promoting plant growth, resilience, and defense mechanisms. By selectively shaping their microbiome, plants harness diverse endophytic bacterial strains to enhance nutrient absorption, regulate hormones, mitigate damage, and contribute to overall plant health. The review underscores the potential of bacterial endophytes in self-sustaining agricultural systems, offering solutions to reduce reliance on fertilizers and pesticides. Additionally, the review highlights the importance of endophytes in enhancing plant tolerance to various environmental stresses, such as drought, salinity, extreme temperatures, and heavy metal toxicity. The review emphasizes the significance of understanding and harnessing the mutualistic relationship between plants and endophytes for maximizing agricultural yields and promoting sustainable farming practices. | Qadir, Muhammad; Iqbal, Amjad; Hussain, Anwar; Hussain, Adil; Shah, Farooq; Yun, Byung-Wook; Mun, Bong-Gyu | Abdul Wali Khan Univ Mardan, Dept Bot, Khyber Pakhtunkhwa 23200, Pakistan; Hunan Univ, Coll Biol, Hunan Key Lab Plant Funct Genom & Dev Regulat, Changsha 410082, Peoples R China; Abdul Wali Khan Univ Mardan, Dept Food Sci & Technol, Khyber Pakhtunkhwa 23200, Pakistan; Abdul Wali Khan Univ Mardan, Dept Entomol, Khyber Pakhtunkhwa 23200, Pakistan; Abdul Wali Khan Univ Mardan, Dept Agron, Khyber Pakhtunkhwa 23200, Pakistan; Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea; Chungbuk Natl Univ, Dept Environm Biochem, Cheongju 28644, Chungbuk, South Korea | Shah, Farooq/GVT-6352-2022; Mun, BongGyu/GYD-6010-2022; Hussain, Anwar/N-1605-2019; Hussain, Dr. Adil/K-6016-2018; Iqbal, Amjad/E-2010-2014; Qadir, Muhammad/AFJ-3919-2022; Hussain, Adil/K-6016-2018 | 57210988627; 10142205100; 35621772200; 41961162600; 35111931300; 8245123600; 57147241300 | qadir1434@gmail.com;munbg@chungbuk.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 25 | 22 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2024 | 4.9 | 22.4 | 1.03 | 2025-05-07 | 6 | 6 | endophytic bacteria; secondary metabolites; plant defense; biofertilizers; biopesticides | INDUCED SYSTEMIC RESISTANCE; DROUGHT-STRESS; DISEASE RESISTANCE; ZEA-MAYS; GROWTH; TOLERANCE; AZOSPIRILLUM; ENDOPHYTES; BACILLUS; SPP. | biofertilizers; biopesticides; endophytic bacteria; plant defense; secondary metabolites | Agriculture; Bacteria; Crops, Agricultural; Endophytes; Microbiota; Plant Development; Plants; Stress, Physiological; Symbiosis; fertilizer; pesticide; abiotic stress; absorption; agriculture; bacterial strain; drought; endophyte; environmental resilience; heavy metal poisoning; nonhuman; pest control; phytopathogen; plant bacterium interaction; plant defense; plant growth; plant stress; Review; salinity; secondary metabolism; sustainable agriculture; symbiosis; temperature; bacterium; crop; growth, development and aging; metabolism; microbiology; microflora; physiological stress; physiology; plant; plant development; procedures | English | 2024 | 2024-11 | 10.3390/ijms252212198 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Functional Characterization of Circadian Nuclear Receptors REV-ERBα and REV-ERBβ in Human Osteosarcoma Cell Cultures | REV-ERB alpha and its paralog, REV-ERB beta, encoded by NR1D1 and NR1D2 genes, are key nuclear receptors that link the circadian timing system and metabolic homeostasis. Since heme is an endogenous ligand, REV-ERBs have been considered key components of the circadian molecular clock and can be pharmacologically targeted to treat various circadian rhythm-related diseases, such as cardiometabolic, inflammatory, and neuropsychiatric diseases, as well as cancer. REV-ERBs are believed to be functionally redundant and compensatory, although they often affect the expression of gene subsets in an isoform-specific manner. Therefore, this study aimed to identify the redundant and distinct roles of each isoform in controlling its target genes by comparing the transcriptome profiles of a panel of mutant U2OS human osteosarcoma cells in which either NR1D1 or NR1D2 was ablated. Indeed, our transcriptomic analyses revealed that most REV-ERB-regulated genes are controlled by redundant or even additive actions. However, the RNA expression profiles of each single mutant cell line also provide strong evidence for isoform-dependent actions. For example, REV-ERB alpha is more responsible for regulating the NF-kappa Beta signaling pathway, whereas a group of extracellular matrix components requires REV-ERB beta to maintain their expression. We found that REV-ERBs have isoform-selective functions in the regulation of certain circadian output pathways despite their overlapping roles in the circadian molecular clock. Thus, the development of isoform-selective REV-ERB modulators can help treat metabolic disturbances and certain types of cancer. | Cho, Hana; Yun, Ahee; Kim, Joohee; Park, Eunjeong; Jung, Jong-Wha; Chung, Sooyoung; Son, Gi Hoon; Bahar, Bojlul | Korea Univ, Coll Med, Dept Biomed Sci, Seoul 02841, South Korea; Ewha Womans Univ, Scranton Coll, Dept Brain & Cognit Sci, Seoul 03760, South Korea; Kyungpook Natl Univ, Coll Pharm, Res Inst Pharmaceut Sci, Daegu 41566, South Korea | 58846335900; 58846408700; 58846408800; 58846408900; 26032128600; 7404292716; 7003374073 | prettyhana1@hanmail.net;aheeyun@gmail.com;kim.jooheejudykim@gmail.com;pinkcolony@korea.ac.kr;jungj@knu.ac.kr;csooy@ewha.ac.kr;songh@korea.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 25 | 2 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2024 | 4.9 | 22.4 | 0 | 2025-04-16 | 1 | 0 | circadian clock; circadian rhythm; REV-ERBs; U2OS cell; transcriptome | TRANSCRIPTION; METABOLISM; BEHAVIOR; RHYTHM; HEME | circadian clock; circadian rhythm; REV-ERBs; transcriptome; U2OS cell | Bone Neoplasms; Cell Culture Techniques; Chronobiology Disorders; Humans; Osteosarcoma; Protein Isoforms; Receptors, Cytoplasmic and Nuclear; cell nucleus receptor; immunoglobulin enhancer binding protein; nuclear receptor NR1D1; nuclear receptor rev erb beta; RNA; transcriptome; unclassified drug; cell receptor; isoprotein; Article; cell culture; cell mutant; comparative study; controlled study; CRISPR-CAS9 system; differential gene expression; extracellular matrix; genetic transcription; genome-wide association study; human; human cell; NF kB signaling; osteosarcoma; osteosarcoma cell; transcriptomics; U2OS cell line; wild type; bone tumor; cell culture technique; genetics; sleep disorder | English | 2024 | 2024-01 | 10.3390/ijms25020770 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Hair Thickness Growth Effect of Adenosine Complex in Male-/Female-Patterned Hair Loss via Inhibition of Androgen Receptor Signaling | Aging (senescence) is an unavoidable biological process that results in visible manifestations in all cutaneous tissues, including scalp skin and hair follicles. Previously, we evaluated the molecular function of adenosine in promoting alopecia treatment in vitro. To elucidate the differences in the molecular mechanisms between minoxidil (MNX) and adenosine, gene expression changes in dermal papilla cells were examined. The androgen receptor (AR) pathway was identified as a candidate target of adenosine for hair growth, and the anti-androgenic activity of adenosine was examined in vitro. In addition, ex vivo examination of human hair follicle organ cultures revealed that adenosine potently elongated the anagen stage. According to the severity of alopecia, the ratio of the two peaks (terminal hair area/vellus hair area) decreased continuously. We further investigated the adenosine hair growth promoting effect in vivo to examine the hair thickness growth effects of topical 5% MNX and the adenosine complex (0.75% adenosine, 1% penthenol, and 2% niacinamide; APN) in vivo. After 4 months of administration, both the MNX and APN group showed significant increases in hair density (MNX + 5.01% (p < 0.01), APN + 6.20% (p < 0.001)) and thickness (MNX + 5.14% (p < 0.001), APN + 10.32% (p < 0.001)). The inhibition of AR signaling via adenosine could have contributed to hair thickness growth. We suggest that the anti-androgenic effect of adenosine, along with the evaluation of hair thickness distribution, could help us to understand hair physiology and to investigate new approaches for drug development. | Kim, Jaeyoon; Shin, Jae young; Choi, Yun-Ho; Joo, Jang Ho; Kwack, Mi Hee; Sung, Young Kwan; Kang, Nae Gyu | LG Household & Hlth Care LG H&H R&D Ctr, 70 Magokjoongang 10 ro, Seoul 07795, South Korea; Kyungpook Natl Univ, Sch Med, Dept Immunol, Daegu 41944, South Korea | ; Kim, Jaeyoon/JZE-2020-2024 | 57211615656; 57220731453; 57211705649; 59195540500; 6507685557; 55663365300; 55658832600 | kjy5281@lghnh.com;sjy2811@lghnh.com;youknow@lghnh.com;janghojoo@lghnh.com;go3004@knu.ac.kr;ysung@knu.ac.kr;ngkang@lghnh.com; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 25 | 12 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2024 | 4.9 | 22.4 | 0.38 | 2025-05-07 | 2 | 2 | adenosine; anti-androgenic activity; hair thickness; androgen receptor; androgen alopecia | INCREASES ANAGEN HAIR; DERMAL PAPILLA CELLS; FOLLICLE MORPHOGENESIS; TOPICAL MINOXIDIL; ALOPECIA; MEN; SKIN; FINASTERIDE; EXPRESSION; APOPTOSIS | adenosine; androgen alopecia; androgen receptor; anti-androgenic activity; hair thickness | Adenosine; Alopecia; Animals; Female; Hair; Hair Follicle; Humans; Male; Minoxidil; Receptors, Androgen; Signal Transduction; adenosine; adenosine A2b receptor; adenosine complex; adenosine receptor; androgen receptor; dexpanthenol; finasteride; heat shock protein 27; melanin; minoxidil; mitogen activated protein kinase; nicotinamide; protein p53; receptor subtype; unclassified drug; adenosine; androgen receptor; minoxidil; 22Rv1 cell line; adult; aging; alopecia; antiandrogen therapy; antibody microarray; Article; cell differentiation; chemical structure; cluster analysis; computer model; controlled study; dermis; ex vivo study; female; gene; gene expression; hair; hair follicle; hair growth; hair loss; hair thickness; human; human cell; immunohistochemistry; in vitro study; luciferase assay; male; MAPK signaling; microdissection; mRNA expression level; organ culture; papillary dermis; phosphorylation; real time polymerase chain reaction; RNA extraction; scalp; senescence; signal transduction; terminal hair; thickness; vellus hair; alopecia; animal; drug effect; drug therapy; growth, development and aging; hair; hair follicle; metabolism; pathology | English | 2024 | 2024-06 | 10.3390/ijms25126534 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Review | High Quantum Yields and Biomedical Fluorescent Imaging Applications of Photosensitized Trivalent Lanthanide Ion-Based Nanoparticles | In recent years, significant advances in enhancing the quantum yield (QY) of trivalent lanthanide (Ln(3+)) ion-based nanoparticles have been achieved through photosensitization, using host matrices or capping organic ligands as photosensitizers to absorb incoming photons and transfer energy to the Ln(3+) ions. The Ln(3+) ion-based nanoparticles possess several excellent fluorescent properties, such as nearly constant transition energies, atomic-like sharp transitions, long emission lifetimes, large Stokes shifts, high photostability, and resistance to photobleaching; these properties make them more promising candidates as next-generation fluorescence probes in the visible region, compared with other traditional materials such as organic dyes and quantum dots. However, their QYs are generally low and thus need to be improved to facilitate and extend their applications. Considerable efforts have been made to improve the QYs of Ln(3+) ion-based nanoparticles through photosensitization. These efforts include the doping of Ln(3+) ions into host matrices or capping the nanoparticles with organic ligands. Among the Ln(3+) ion-based nanoparticles investigated in previous studies, this review focuses on those containing Eu3+, Tb3+, and Dy3+ ions with red, green, and yellow emission colors, respectively. The emission intensities of Eu3+ and Tb3+ ions are stronger than those of other Ln3+ ions; therefore, the majority of the reported studies focused on Eu3+ and Tb3+ ion-based nanoparticles. This review discusses the principles of photosensitization, several examples of photosensitized Ln(3+) ion-based nanoparticles, and in vitro and in vivo biomedical fluorescent imaging (FI) applications. This information provides valuable insight into the development of Ln(3+) ion-based nanoparticles with high QYs through photosensitization, with future potential applications in biomedical FI. | Tegafaw, Tirusew; Zhao, Dejun; Liu, Ying; Yue, Huan; Saidi, Abdullah Khamis Ali Al; Baek, Ahrum; Kim, Jihyun; Chang, Yongmin; Lee, Gang Ho | Kyungpook Natl Univ, Coll Nat Sci, Dept Chem, Taegu 41566, South Korea; Kyungpook Natl Univ, Dept Med & Biol Engn, Taegu 41944, South Korea; Kyungpook Natl Univ, Teachers Coll, Dept Chem Educ, Taegu 41566, South Korea; Kyungpook Natl Univ, Sch Med, Dept Mol Med, Taegu 41944, South Korea | 55983618600; 57222567792; 57221731093; 57200329016; 57452796700; 57194601711; 56954528100; 7501840633; 7404851841 | tirukorea@gmail.com;djzhao.chem@gmail.com;ly1124161@gmail.com;yuehuan888@gmail.com;abdullah_al_saidi@hotmail.com;baxun@naver.com;jkim23@knu.ac.kr;ychang@knu.ac.kr;ghlee@mail.knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 25 | 21 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2024 | 4.9 | 22.4 | 0 | 2025-05-07 | 1 | 1 | Ln(3+) ion-based nanoparticle; photosensitization; host matrix; organic ligand; high quantum yield; biomedical fluorescent imaging application | MICROWAVE-ASSISTED SYNTHESIS; LUMINESCENT LAPO4-CE,TB NANOCRYSTALS; HOST-SENSITIZED LUMINESCENCE; RED-EMITTING PHOSPHOR; LIQUID BASED APPROACH; ENERGY-TRANSFER; IN-VITRO; TUNABLE LUMINESCENCE; TEMPERATURE SYNTHESIS; ROOM-TEMPERATURE | biomedical fluorescent imaging application; high quantum yield; host matrix; Ln<sup>3+</sup> ion-based nanoparticle; organic ligand; photosensitization | Animals; Fluorescent Dyes; Humans; Ions; Lanthanoid Series Elements; Nanoparticles; Optical Imaging; Photosensitizing Agents; Quantum Dots; dye; fluorescent dye; ion; lanthanide; ligand; nanoparticle; photosensitizing agent; quantum dot; ion; photosensitizing agent; bleaching; color; controlled study; drug development; electric potential; energy transfer; fluorescence; imaging software; in vitro study; nonhuman; pharmaceutics; pharmacology; photon; photosensitization; quantum yield; review; animal; chemistry; fluorescence imaging; human; procedures | English | 2024 | 2024-11 | 10.3390/ijms252111419 | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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