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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | ○ | Article | Anti-Melanogenic Effects of Korean Red Ginseng Oil in an Ultraviolet B-Induced Hairless Mouse Model | A 'remedy for all' natural product widely known in the Korean Peninsula is called Panax Ginseng Meyer. Globalization represents a persistent risk to the ozone layer, leading to bountiful amounts of Ultra-Violet B beams (UVB). The variety in human skin hues is ascribed to the characteristic color called Melanin. However, Melanin overproduction due to UVB beams promotes skin staining and tumorigenesis, a process called photo aging, which damages skin quality. To assess the effects of Korean Red Ginseng Oil (KGO) on photo aging, the murine melanoma cell lines B16/F10 were used in vitro and HRM-2 hairless mice exposed to UVB were studied in vivo. Our results revealed that KGO reduced tyrosinase activity and melanin production in B16/F10 cells along with the suppression of upstream factors involved in the melanin production pathway, both transcriptionally and transitionally. In the in vivo studies, KGO suppressed the expression of Matrix Metalloproteinase (MMP) and Interleukins along with a reduction of depth in wrinkle formation and reduced collagen degradation. Moreover, the feed intake and feed efficiency ratio that decreased as a result of UVB exposure was also improved by KGO treatment. In light of our results, we conclude that KGO can have considerable benefits due to its various properties of natural skin enhancement. | Saba, Evelyn; Kim, Seung-Hyung; Lee, Yuan Yee; Kim, Hyun-Kyoung; Roh, Seong-Soo; Kwak, Yi-Seong; Park, Chae-Kyu; Kim, Sung-Dae; Rhee, Man Hee | Kyungpook Natl Univ, Coll Vet Med, Dept Vet Med, Daegu 41566, South Korea; Daejeon Univ, Inst Tradit Med & Biosci, Daejeon 34520, South Korea; Seowon Univ, Dept Food Sci & Engn, Chungbuk 28674, South Korea; Daegu Haany Univ, Coll Korean Med, Daegu 42158, South Korea; Korean Ginseng Cooperat, R&D Headquarters, Daejeon 34520, South Korea; Dongnam Inst Radiol & Med Sci, Res Ctr, Busan 46033, South Korea; Pir Mehr Ali Shah Arid Agr Univ, Rawalpindi 46000, Pakistan | ; Yuan Yee, Lee/ABH-8956-2022; Kim, Seung-Hyung/AAA-4707-2020; Saba, Evelyn/JLN-1878-2023; Rhee, Man/O-5705-2016; Kim, Hyun/AAT-6695-2021 | 56721112000; 54383305300; 57203798815; 55791359100; 12752302700; 36868130200; 55885553100; 55156746000; 57211035357 | evelyn.saba@uaar.edu.pk;sksh518@dju.kr;yuanyeelee@knu.ac.kr;kimhk4@seowon.ac.kr;ddede@dhu.ac.kr;twostar@kgc.or.kr;ckpark@kgc.co.kr;sdkim@dirams.co.kr;rheemh@knu.ac.kr; | MOLECULES | MOLECULES | 1420-3049 | 25 | 20 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 4.412 | 35.1 | 0.49 | 2025-06-25 | 9 | 9 | Korean red ginseng oil ointment; melanin; wrinkles; antiaging; collagen | MELANIN; MECHANISMS; TYROSINASE; SKIN | Antiaging; Collagen; Korean red ginseng oil ointment; Melanin; Wrinkles | Animals; Carcinogenesis; Fibroblasts; Humans; Melanins; Melanoma, Experimental; Mice; Mice, Hairless; Ozone; Panax; Plant Extracts; Plant Oils; Skin; Ultraviolet Rays; melanin; ozone; plant extract; vegetable oil; adverse event; animal; biosynthesis; carcinogenesis; chemistry; drug effect; experimental melanoma; fibroblast; hairless mouse; human; metabolism; mouse; Panax; radiation response; skin; ultraviolet radiation | English | 2020 | 2020-10 | 10.3390/molecules25204755 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Bacillus amyloliquefaciens RWL-1 as a New Potential Strain for Augmenting Biochemical and Nutritional Composition of Fermented Soybean | Soybean (Glycine max L.) is a good source of natural antioxidants and commonly consumed as fermented products such as cheonggukjang, miso, tempeh, and sufu in Asian countries. The aim of the current study was to examine the influence of novel endophytic bacterial strain, Bacillus amyloliquefaciens RWL-1 as a starter for soybean fermentation. During fermentation, the cooked soybeans were inoculated with different concentrations (1%, 3%, and 5%) of B. amyloliquefaciens RWL-1. The changes in 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2 ' -azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activities, total phenolic contents, isoflavones (Daidzin, Genistin, Glycitin, Daidzein, Glycitein, and Genistein), amino acids (aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cysteine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine, arginine, and proline) composition, and minerals (calcium, copper, iron, potassium, magnesium, manganese, sodium, nickel, lead, arsenic, and zinc) were investigated. The level of antioxidants, total phenolic contents, isoflavones, and total amino acids were higher in fermented soybean inoculated with 1% B. amyloliquefaciens RWL-1 after 60 h of fermentation as compared to control, 3% and 5% B. amyloliquefaciens RWL-1. Additionally, fermented soybean inoculated with 5% B. amyloliquefaciens RWL-1 showed the highest values for mineral contents. Changes in antioxidant activities and bioactive compounds depended on the concentration of the strain used for fermentation. From these results, we conclude that fermented soybean has strong antioxidant activity, probably due to its increased total phenolic contents and aglycone isoflavone that resulted from fermentation. Such natural antioxidants could be used in drug and food industries and can be considered to alleviate oxidative stress. | Shahzad, Raheem; Shehzad, Adeeb; Bilal, Saqib; Lee, In-Jung | Imam Abdulrahman Bin Faisal Univ, Basic & Appl Sci Res Ctr, POB 1982, Dammam 31441, Saudi Arabia; Imam Abdulrahman Bin Faisal Univ, Coll Sci, Dept Biol, POB 1982, Dammam 31441, Saudi Arabia; Imam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat IRMC, Dept Clin Pharm, POB 1982, Dammam 31441, Saudi Arabia; Univ Nizwa, Nat & Med Sci Res Ctr, Nizwa 616, Oman; Kyungpook Natl Univ, Sch Appl Biosci, Daegu 41566, South Korea | Lee, In-Jung/GLS-0432-2022; Shahzad, Raheem/AAG-8370-2019; Shehzad, Adeeb/HHN-4847-2022 | 56454250900; 36162526700; 57031617400; 16425830900 | rmshahzad@iau.edu.sa;asmsiar@iau.edu.sa;saqib043@yahoo.com;ijlee@knu.ac.kr; | MOLECULES | MOLECULES | 1420-3049 | 25 | 10 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 4.412 | 35.1 | 1.72 | 2025-06-25 | 31 | 34 | fermented soybean; fermentation; antioxidant activity; amino acid; isoflavones; nutritional composition | AMINO-ACID PROFILES; ISOFLAVONE AGLYCONES; IN-VITRO; ANTIOXIDANT; CHEONGGUKJANG; ENHANCEMENT; VARIETIES; STRENGTH; TOFU | Amino acid; Antioxidant activity; Fermentation; Fermented soybean; Isoflavones; Nutritional composition | Amino Acids; Antioxidants; Bacillus amyloliquefaciens; Fermentation; Food Hypersensitivity; Genistein; Isoflavones; Nutritive Value; Phenols; Soybeans; amino acid; antioxidant; daidzein; daidzin; genistein; genistin; glycitein; isoflavone derivative; phenol derivative; Bacillus amyloliquefaciens; chemistry; fermentation; food allergy; metabolism; microbiology; nutritional value; soybean | English | 2020 | 2020-05 | 10.3390/molecules25102346 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Bioanalytical Method Using Ultra-High-Performance Liquid Chromatography Coupled with High-Resolution Mass Spectrometry (UHPL-CHRMS) for the Detection of Metformin in Human Plasma | Metformin is the first-line medicine for the treatment of type 2 diabetes. Drug interactions between metformin and other drugs, food, or beverages cannot only cause changes in the pharmacokinetic profiles but also affect the efficacy of metformin. The purpose of this study was to develop a rapid and reliable bioanalytical method for the detection of plasma metformin concentration in humans. To remove interfering substances in plasma, acidified acetonitrile (acetonitrile containing 0.1% formic acid) was added to samples. Ultra-high-performance liquid chromatography (UHPLC) coupled with high resolution mass spectrometry (HRMS) was used to analyze metformin and its internal standard (metformin-d6). Analyte separation was performed on a BEH HILIC analytical column (100 x 2.1 mm, 1.7 mu m) using a gradient elution of 0.1% formic acid (A) and acetonitrile with 0.1% formic acid (B). The total chromatographic run time was 2 min. The developed method was validated for its linearity, accuracy and precision, selectivity (signal of interfering substance; analyte, lower limit of quantification (LLOQ) = 10), stability (low quality control (LQC, 15 ng/mL), 2.95-14.19%; high quality control (HQC, 1600 ng/mL), -9.49-15.10%), dilution integrity (diluted QC (4000 ng/mL); 10-folds diluted QC (400 ng/mL); 5-folds diluted QC (800 ng/mL); accuracy, 81.30-91.98%; precision, <= 4.47%), carry-over (signal of double blank; analyte, LLOQ <= 20%; IS, IS <= 5%), and matrix effect (LQC, 10.109%; HQC, 12.271%) under various conditions. The constructed calibration curves were shown linear in the concentration range of 5-2000 ng/mL, with within- and between-run precision values of <8.19% and accuracy in the range of 91.13-105.25%. The plasma metformin concentration of 16 healthy subjects was successfully measured by applying the validated bioanalytical method. | Kang, Ye-Ji; Jeong, Hyeon-Cheol; Kim, Tae-Eun; Shin, Kwang-Hee | Kyungpook Natl Univ, Coll Pharm, Res Inst Pharmaceut Sci, Daegu 41566, South Korea; Konkuk Univ, Med Ctr, Dept Clin Pharmacol, Seoul 05029, South Korea | Kim, Tae/I-6368-2018 | 57219382830; 57196346934; 7407122618; 35216279300 | yeyezzy@knu.ac.kr;houkiboshi01@knu.ac.kr;tekim@kuh.ac.kr;kshin@knu.ac.kr; | MOLECULES | MOLECULES | 1420-3049 | 25 | 20 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 4.412 | 35.1 | 0.49 | 2025-06-25 | 14 | 15 | metformin; bioanalytical method; ultra-high-performance liquid chromatography (UHPLC); high-resolution mass spectrometry (HRMS); HILIC | QUANTITATIVE PERFORMANCE; ORBITRAP; DRUGS | Bioanalytical method; High-resolution mass spectrometry (HRMS); HILIC; Metformin; Ultra-high-performance liquid chromatography (UHPLC) | Chromatography, High Pressure Liquid; Humans; Metformin; Tandem Mass Spectrometry; metformin; blood; high performance liquid chromatography; human; procedures; tandem mass spectrometry | English | 2020 | 2020-10 | 10.3390/molecules25204625 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | d-allulose Ameliorates Metabolic Dysfunction in C57BL/KsJ-db/db Mice | d-allulose is an uncommon sugar that provides almost no calories when consumed. Its sweetness is 70% that of sucrose.d-allulose is a metabolic regulator of glucose and lipid metabolism. However, few reports concerning its effect on diabetes and related metabolic disturbances in db/db mice are available. In this study, we evaluatedd-allulose's effect on hyperglycemia, hyperinsulinemia, diabetes and inflammatory responses in C57BL/KsJ-db/db mice. Mice were divided into normal diet, erythritol supplemented (5%w/w), andd-allulose supplemented (5%w/w) groups. Blood glucose and plasma glucagon levels and homeostatic model assessment (HOMA-IR) were significantly lower in thed-allulose group than in the normal diet group, and plasma insulin level was significantly increased. Further,d-allulose supplement significantly increased hepatic glucokinase activity and decreased hepatic phosphoenolpyruvate carboxykinase and glucose-6-phosphatase activity. Expression of glucose transporter 4, insulin receptor substrate 1, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha and AKT serine/threonine kinase 2 were also upregulated byd-allulose supplement in adipocyte and muscle. Finally,d-allulose effectively lowered plasma and hepatic triglyceride and free fatty acid levels, and simultaneously reduced hepatic fatty acid oxidation and carnitine palmitoyl transferase activity. These changes are likely attributable to suppression of hepatic fatty acid synthase and glucose-6-phosphate dehydrogenase activity. Notably,d-allulose also reduced pro-inflammatory adipokine and cytokine levels in plasma. Our results indicate thatd-allulose is an effective sugar substitute for improving lipid and glucose metabolism. | Lee, Dayoun; Han, Youngji; Kwon, Eun-Young; Choi, Myung-Sook | Kyungpook Natl Univ, Dept Food Sci & Nutr, 1370 San Kyuk Dong Puk Ku, Daegu 702701, South Korea; Kyungpook Natl Univ, Ctr Food & Nutrit Genom Res, 1370 San Kyuk Dong Puk Ku, Daegu 702701, South Korea | 57218550537; 57206914262; 15765422500; 7402093877 | dayoon1746@naver.com;youngji.kor.han@gmail.com;eykwon@knu.ac.kr;mschoi@knu.ac.kr; | MOLECULES | MOLECULES | 1420-3049 | 25 | 16 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 4.412 | 35.1 | 1.39 | 2025-06-25 | 22 | 25 | type 2 diabetes mellitus; d-allulose; sugar substitute; metabolic syndrome | FREE FATTY-ACIDS; INSULIN-RESISTANCE; LIPID-METABOLISM; TNF-ALPHA; OBESITY; GLUCOSE; LEPTIN; INFLAMMATION; PATHOGENESIS; COMPLICATIONS | D-allulose; Metabolic syndrome; Sugar substitute; Type 2 diabetes mellitus | Animals; Blood Glucose; Diabetes Mellitus, Experimental; Fructose; Hyperglycemia; Hyperinsulinism; Inflammation; Insulin; Lipids; Male; Mice; Mice, Inbred C57BL; Mice, Inbred Strains; fructose; insulin; lipid; psicose; animal; blood; C57BL mouse; experimental diabetes mellitus; glucose blood level; hyperglycemia; hyperinsulinism; inbred mouse strain; inflammation; male; metabolism; mouse; pathology | English | 2020 | 2020-08 | 10.3390/molecules25163656 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Gamma Aminobutyric Acid-Enriched Fermented Oyster (Crassostrea gigas) Increases the Length of the Growth Plate on the Proximal Tibia Bone in Sprague-Dawley Rats | Bone growth during childhood and puberty determines an adult's final stature. Although several prior studies have reported that fermented oyster (FO) consisting of a high amount of gamma aminobutyric acid can be attributed to bone health, there is no research on the efficacy of FO on growth regulation and the proximal tibial growth plate. Therefore, in this study, we investigated the effect of FO oral administration on hepatic and serum growth regulator levels and the development of the proximal tibial growth plate in young Sprague-Dawley rats. Both oral administration of FO (FO 100, 100 mg/kg FO and FO 200, 200 mg/kg FO) and subcutaneous injection of recombinant human growth hormone (rhGH, 200 mu g/kg of rhGH) for two weeks showed no toxicity. Circulating levels of growth hormone (GH) significantly increased in the FO 200 group. The expression and secretion of insulin-like growth factor-1 (IGF-1) and insulin-like growth factor binding protein-3 (IGFBP-3) were enhanced by FO administration. FO administration promoted the expression of bone morphogenic proteins IGF-1 and IGFBP-3 in the proximal tibial growth plate. This positive effect of FO resulted in incremental growth of the entire plate length by expanding the proliferating and hypertrophic zones in the proximal tibial growth plate. Collectively, our results suggested that oral administration of FO is beneficial for bone health, which may ultimately result in increased height. | Lee, Hyesook; Hwangbo, Hyun; Ji, Seon Yeong; Kim, Min Yeong; Kim, So Young; Kim, Da Hye; Hong, Su Hyun; Lee, Su Jeong; Assefa, Freshet; Kim, Gi-Young; Park, Eui Kyun; Park, Joung-Hyun; Lee, Bae-Jin; Jeon, You-Jin; Choi, Yung Hyun | Dong Eui Univ, Antiaging Res Ctr, Busan 47340, South Korea; Dong Eui Univ, Coll Korean Med, Dept Biochem, Busan 47227, South Korea; Pusan Natl Univ, Dept Mol Biol, Busan 46241, South Korea; Dong Eui Univ, Dept Smart Biohlth, Busan 47340, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Pathol & Regenerat Med, Daegu 41940, South Korea; Jeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea; Marine Bioproc Co Ltd, Ocean Fisheries & Biol Ctr, Busan 46048, South Korea | ; Kim, Kyoung-Sook/A-7768-2017; Lee, sujeong/KUD-4735-2024; Kim, So-Young/JFS-7698-2023; Jeon, You-Jin/AAD-3452-2021 | 57192499270; 57201497483; 57196357007; 57196352735; 57190262437; 57221629878; 37030297500; 57219236074; 57219236123; 7403063801; 37071072400; 53871782200; 35620558000; 55782690600; 57211727369 | 14769@deu.ac.kr;hbhyun2003@naver.com;14602@deu.ac.kr;ilytoo365@deu.ac.kr;14731@deu.ac.kr;believe0402@naver.com;hongsh@deu.ac.kr;marhaul@naver.com;fresheta@gmail.com;epark@knu.ac.kr;pdc327@hanmail.net;hansola82@hanmail.net;youjinj@jejunu.ac.kr;choiyh@deu.ac.kr; | MOLECULES | MOLECULES | 1420-3049 | 25 | 19 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 4.412 | 35.1 | 0.41 | 2025-06-25 | 6 | 6 | fermented oyster; gamma aminobutyric acid; insulin like growth factor-1; recombinant human growth hormone; tibial growth plate | AMINO-ACID; HORMONE; SERUM; GABA; IGF; PROTEINS; SYSTEM; SAUCE; MICE; BMP2 | Fermented oyster; Gamma aminobutyric acid; Insulin like growth factor-1; Recombinant human growth hormone; Tibial growth plate | Animals; Bone Morphogenetic Proteins; Crassostrea; Fermentation; gamma-Aminobutyric Acid; Gene Expression Regulation; Growth Hormone; Growth Plate; Insulin-Like Growth Factor Binding Protein 3; Insulin-Like Growth Factor I; Organ Size; Rats; Rats, Sprague-Dawley; Tibia; 4 aminobutyric acid; bone morphogenetic protein; growth hormone; somatomedin binding protein 3; somatomedin C; animal; blood; chemistry; Crassostrea; drug effect; epiphysis plate; fermentation; gene expression regulation; growth, development and aging; metabolism; organ size; rat; Sprague Dawley rat; tibia | English | 2020 | 2020-10 | 10.3390/molecules25194375 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Herb-Drug Interaction of Red Ginseng Extract and Ginsenoside Rc with Valsartan in Rats | The purpose of this study was to investigate the herb-drug interactions involving red ginseng extract (RGE) or ginsenoside Rc with valsartan, a substrate for organic anion transporting polypeptide (OATP/Oatp) transporters. In HEK293 cells overexpressing drug transporters, the protopanaxadiol (PPD)-type ginsenosides- Rb1, Rb2, Rc, Rd, Rg3, compound K, and Rh2-inhibited human OATP1B1 and OATP1B3 transporters (IC50 values of 7.99-68.2 mu M for OATP1B1; 1.36-30.8 mu M for OATP1B3), suggesting the herb-drug interaction of PPD-type ginsenosides involving OATPs. Protopanaxatriol (PPT)-type ginsenosides-Re, Rg1, and Rh1-did not inhibit OATP1B1 and OATP1B3 and all ginsenosides tested didn't inhibit OCT and OAT transporters. However, in rats, neither RGE nor Rc, a potent OATP inhibitor among PPD-type ginsenoside, changed in vivo pharmacokinetics of valsartan following repeated oral administration of RGE (1.5 g/kg/day for 7 days) or repeated intravenous injection of Rc (3 mg/kg for 5 days). The lack of in vivo herb-drug interaction between orally administered RGE and valsartan could be attributed to the low plasma concentration of PPD-type ginsenosides (5.3-48.4 nM). Even high plasma concentration of Rc did not effectively alter the pharmacokinetics of valsartan because of high protein binding and the limited liver distribution of Rc. The results, in conclusion, would provide useful information for herb-drug interaction between RGE or PPD-type ginsenosides and Oatp substrate drugs. | Jeon, Ji-Hyeon; Lee, Sowon; Lee, Wonpyo; Jin, Sojeong; Kwon, Mihwa; Shin, Chul Hwi; Choi, Min-Koo; Song, Im-Sook | Kyungpook Natl Univ, Coll Pharm, Daegu 41566, South Korea; Kyungpook Natl Univ, Res Inst Pharmaceut Sci, Daegu 41566, South Korea; Dankook Univ, Coll Pharm, Cheonan 31116, South Korea | 57204685946; 57204650133; 57202872476; 57204690167; 55964714000; 57214674287; 8695781400; 7201564500 | kei7016@naver.com;okjin917@hanmail.net;woopyo906@gmail.com;astraea327@naver.com;mihwa_k@naver.com;tusshinn@gmail.com;minkoochoi@dankook.ac.kr;isssong@knu.ac.kr; | MOLECULES | MOLECULES | 1420-3049 | 25 | 3 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 4.412 | 35.1 | 2.21 | 2025-06-25 | 32 | 34 | red ginseng extract (RGE); ginsenoside Rc; herb-drug interaction; organic anion transporting polypeptide (Oatp); valsartan | ORGANIC CATION TRANSPORTERS; PANAX-GINSENG; IN-VITRO; MOLECULAR-MECHANISMS; PHARMACOKINETICS; PLASMA; DISPOSITION; COMPONENTS; RIFAMPICIN | Ginsenoside Rc; Herb–drug interaction; Organic anion transporting polypeptide (Oatp); Red ginseng extract (RGE); Valsartan | Administration, Oral; Animals; Down-Regulation; Gene Expression Regulation; Ginsenosides; HEK293 Cells; Herb-Drug Interactions; Humans; Liver-Specific Organic Anion Transporter 1; Male; Rats; Solute Carrier Organic Anion Transporter Family Member 1B3; Valsartan; ginsenoside; ginsenoside M1; ginsenoside Rb 1; ginsenoside Rb 2; ginsenoside Rc; ginsenoside Re; ginsenoside Rg 3; SLCO1B1 protein, human; SLCO1B3 protein, human; solute carrier organic anion transporter 1B3; valsartan; animal; down regulation; drug effect; gene expression regulation; genetics; HEK293 cell line; herb drug interaction; human; male; oral drug administration; rat | English | 2020 | 2020-02-01 | 10.3390/molecules25030622 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | In Vitro Interaction of AB-FUBINACA with Human Cytochrome P450, UDP-Glucuronosyltransferase Enzymes and Drug Transporters | AB-FUBINACA, a synthetic indazole carboxamide cannabinoid, has been used worldwide as a new psychoactive substance. Because drug abusers take various drugs concomitantly, it is necessary to explore potential AB-FUBINACA-induced drug-drug interactions caused by modulation of drug-metabolizing enzymes and transporters. In this study, the inhibitory effects of AB-FUBINACA on eight major human cytochrome P450s (CYPs) and six uridine 5 '-diphospho-glucuronosyltransferases (UGTs) of human liver microsomes, and on eight clinically important transport activities including organic cation transporters (OCT)1 and OCT2, organic anion transporters (OAT)1 and OAT3, organic anion transporting polypeptide transporters (OATP)1B1 and OATP1B3, P-glycoprotein, and breast cancer resistance protein (BCRP) in transporter-overexpressing cells were investigated. AB-FUBINACA inhibited CYP2B6-mediated bupropion hydroxylation via mixed inhibition with K-i value of 15.0 mu M and competitively inhibited CYP2C8-catalyzed amodiaquine N-de-ethylation, CYP2C9-catalyzed diclofenac 4 '-hydroxylation, CYP2C19-catalyzed [S]-mephenytoin 4 '-hydroxylation, and CYP2D6-catalyzed bufuralol 1 '-hydroxylation with K-i values of 19.9, 13.1, 6.3, and 20.8 mu M, respectively. AB-FUBINACA inhibited OCT2-mediated MPP+ uptake via mixed inhibition (K-i, 54.2 mu M) and competitively inhibited OATP1B1-mediated estrone-3-sulfate uptake (K-i, 94.4 mu M). However, AB-FUBINACA did not significantly inhibit CYP1A2, CYP2A6, CYP3A4, UGT1A1, UGT1A3, UGT1A4, UGT1A6, or UGT2B7 enzyme activities at concentrations up to 100 mu M. AB-FUBINACA did not significantly inhibit the transport activities of OCT1, OAT1/3, OATP1B3, P-glycoprotein, or BCRP at concentrations up to 250 mu M. As the pharmacokinetics of AB-FUBINACA in humans and animals remain unknown, it is necessary to clinically evaluate potential in vivo pharmacokinetic drug-drug interactions induced by AB-FUBINACA-mediated inhibition of CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, OCT2, and OATP1B1 activities. | Kim, Sunjoo; Kim, Dong Kyun; Shin, Yongho; Jeon, Ji-Hyeon; Song, Im-Sook; Lee, Hye Suk | Catholic Univ Korea, Drug Metab & Bioanal Lab, Coll Pharm, Bucheon 14662, South Korea; Kyungpook Natl Univ, Coll Pharm, Daegu 41566, South Korea; Kyungpook Natl Univ, Pharmaceut Sci Res Inst, Daegu 41566, South Korea | ; Shin, Yongho/HKO-5185-2023 | 57051715600; 55742880300; 57206634976; 57204685946; 7201564500; 35316111800 | sjkim712@catholic.ac.kr;kdk3124@catholic.ac.kr;driger6103@catholic.ac.kr;kei7016@naver.com;isssong@knu.ac.kr;sianalee@catholic.ac.kr; | MOLECULES | MOLECULES | 1420-3049 | 25 | 19 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 4.412 | 35.1 | 0.25 | 2025-06-25 | 6 | 5 | AB-FUBINACA; drug-drug interaction; cytochrome P450; uridine 5 '-diphosphoglucuronosyltransferases; drug transporters | SYNTHETIC CANNABINOIDS; PSYCHOACTIVE SUBSTANCES; MAJOR PHYTOCANNABINOIDS; ADB-FUBINACA; CANNABIDIOL; METABOLISM; INHIBITOR; PINACA; ABUSE; FLUID | AB-FUBINACA; Cytochrome P450; Drug transporters; Drug-drug interaction; Uridine 5<sup>0</sup>-diphospho-glucuronosyltransferases | Cannabinoids; Cell Line; Cytochrome P-450 Enzyme Inhibitors; Cytochrome P-450 Enzyme System; Drug Interactions; Glucuronosyltransferase; HEK293 Cells; Humans; Indazoles; Membrane Transport Proteins; Microsomes, Liver; Uridine Diphosphate; AB-FUBINACA; cannabinoid; carrier protein; cytochrome P450; cytochrome P450 inhibitor; glucuronosyltransferase; indazole derivative; uridine diphosphate; cell line; drug interaction; HEK293 cell line; human; liver microsome; metabolism; physiology | English | 2020 | 2020-10 | 10.3390/molecules25194589 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | Article | In vivo positive magnetic resonance imaging applications of poly(methyl vinyl ether-alt-maleic acid)-coated ultra-small paramagnetic gadolinium oxide nanoparticles | The study of ultra-small paramagnetic gadolinium oxide (Gd2O3) nanoparticles (NPs) as in vivo positive (T1) magnetic resonance imaging (MRI) contrast agents is one of the most attractive fields in nanomedicine. The performance of the Gd2O3 NP imaging agents depends on the surface-coating materials. In this study, poly(methyl vinyl ether-alt-maleic acid) (PMVEMA) was used as a surface-coating polymer. The PMVEMA-coated paramagnetic ultra-small Gd2O3 NPs with an average particle diameter of 1.9 nm were synthesized using the one-pot polyol method. They exhibited excellent colloidal stability inwater and good biocompatibility. They also showed a very high longitudinal water proton spin relaxivity (r1) value of 36.2 s-1mM-1 (r2/r1 = 2.0; r2 = transverse water proton spin relaxivity) under a 3.0 tesla MR field which is approximately 10 times higher than the r1 values of commercial molecular contrast agents. High positive contrast enhancements were observed in in vivo T1 MR images after intravenous administration of the NP solution sample, demonstrating its potential as a T1 MRI contrast agent. © 2020 by the authors. | Ahmad, Mohammad Yaseen; Ahmad, Md. Wasi; Yue, Huan; Ho, Son Long; Park, Ji Ae; Jung, Ki-Hye; Cha, Hyunsil; Marasini, Shanti; Ghazanfari, Adibehalsadat; Liu, Shuwen; Tegafaw, Tirusew; Chae, Kwon-Seok; Chang, Yongmin; Lee, Gang Ho | Department of Chemistry and Department of Nanoscience and Nanotechnology (DNN), College of Natural Sciences, Kyungpook National University (KNU), Taegu, 41566, South Korea; Department of Chemistry and Department of Nanoscience and Nanotechnology (DNN), College of Natural Sciences, Kyungpook National University (KNU), Taegu, 41566, South Korea, Department of Chemical Engineering, College of Engineering, Dhofar University, P. O. Box 2509, Salalah, 211, Oman; Department of Chemistry and Department of Nanoscience and Nanotechnology (DNN), College of Natural Sciences, Kyungpook National University (KNU), Taegu, 41566, South Korea; Department of Chemistry and Department of Nanoscience and Nanotechnology (DNN), College of Natural Sciences, Kyungpook National University (KNU), Taegu, 41566, South Korea; Division of RI-Convergence Research, Korea Institute of Radiological and Medical Science (KIRAMS), Seoul, 01817, South Korea; Division of RI-Convergence Research, Korea Institute of Radiological and Medical Science (KIRAMS), Seoul, 01817, South Korea; Department of Molecular Medicine and Medical and Biological Engineering and DNN, School of Medicine, KNU and Hospital, Taegu, 41566, South Korea; Department of Chemistry and Department of Nanoscience and Nanotechnology (DNN), College of Natural Sciences, Kyungpook National University (KNU), Taegu, 41566, South Korea; Department of Chemistry and Department of Nanoscience and Nanotechnology (DNN), College of Natural Sciences, Kyungpook National University (KNU), Taegu, 41566, South Korea; Department of Chemistry and Department of Nanoscience and Nanotechnology (DNN), College of Natural Sciences, Kyungpook National University (KNU), Taegu, 41566, South Korea; Department of Chemistry and Department of Nanoscience and Nanotechnology (DNN), College of Natural Sciences, Kyungpook National University (KNU), Taegu, 41566, South Korea; Department of Biology Education and DNN, Teachers' College, KNU, Taegu, 41566, South Korea; Department of Molecular Medicine and Medical and Biological Engineering and DNN, School of Medicine, KNU and Hospital, Taegu, 41566, South Korea; Department of Chemistry and Department of Nanoscience and Nanotechnology (DNN), College of Natural Sciences, Kyungpook National University (KNU), Taegu, 41566, South Korea | 57203054570; 59107859000; 57200329016; 55659242700; 16319690600; 53865234900; 57189728122; 57200329199; 57200327606; 57208926248; 55983618600; 15743626400; 7501840633; 7404851841 | ghlee@mail.knu.ac.kr; | Molecules | MOLECULES | N/A | 1420-3049 | 25 | 5 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 4.412 | 35.1 | 1.72 | 2025-06-25 | 26 | Contrast agent; Paramagnetic; Poly (methyl vinyl ether-alt-maleic acid); T1 magnetic resonance imaging; Ultra-small Gd<sub>2</sub>O<sub>3</sub> nanoparticle | Animals; Cell Line, Tumor; Cell Survival; Chemical Phenomena; Coated Materials, Biocompatible; Contrast Media; Gadolinium; Magnetic Resonance Imaging; Maleic Anhydrides; Metal Nanoparticles; Mice; Molecular Structure; Particle Size; Polyvinyls; Signal-To-Noise Ratio; Spectrum Analysis; biocompatible coated material; contrast medium; gadolinium; gadolinium oxide; maleic anhydride; metal nanoparticle; poly(methyl vinyl ether-alt-maleic acid); polyvinyl derivative; animal; cell survival; chemical phenomena; chemical structure; chemistry; mouse; nuclear magnetic resonance imaging; particle size; procedures; signal noise ratio; spectroscopy; tumor cell line; ultrastructure | English | Final | 2020 | 10.3390/molecules25051159 | 바로가기 | 바로가기 | 바로가기 | |||||||
| ○ | ○ | Review | Intranasal Delivery of Nanoformulations: A Potential Way of Treatment for Neurological Disorders | Although the global prevalence of neurological disorders such as Parkinson's disease, Alzheimer's disease, glioblastoma, epilepsy, and multiple sclerosis is steadily increasing, effective delivery of drug molecules in therapeutic quantities to the central nervous system (CNS) is still lacking. The blood brain barrier (BBB) is the major obstacle for the entry of drugs into the brain, as it comprises a tight layer of endothelial cells surrounded by astrocyte foot processes that limit drugs' entry. In recent times, intranasal drug delivery has emerged as a reliable method to bypass the BBB and treat neurological diseases. The intranasal route for drug delivery to the brain with both solution and particulate formulations has been demonstrated repeatedly in preclinical models, including in human trials. The key features determining the efficacy of drug delivery via the intranasal route include delivery to the olfactory area of the nares, a longer retention time at the nasal mucosal surface, enhanced penetration of the drugs through the nasal epithelia, and reduced drug metabolism in the nasal cavity. This review describes important neurological disorders, challenges in drug delivery to the disordered CNS, and new nasal delivery techniques designed to overcome these challenges and facilitate more efficient and targeted drug delivery. The potential for treatment possibilities with intranasal transfer of drugs will increase with the development of more effective formulations and delivery devices. | Ul Islam, Salman; Shehzad, Adeeb; Ahmed, Muhammad Bilal; Lee, Young Sup | Kyungpook Natl Univ, Coll Nat Sci, Sch Life Sci, Daegu 41566, South Korea; Imam Abdulrahman bin Faisal Univ, Inst Res & Med Consultat IRMC, Dept Clin Pharm, Dammam 31441, Saudi Arabia | Shehzad, Adeeb/HHN-4847-2022 | 56985186700; 36162526700; 58689879600; 36013628200 | dr_ssulman@yahoo.com;asmsiar@iau.edu.sa;mbilalknu@gmail.com;yselee@knu.ac.kr; | MOLECULES | MOLECULES | 1420-3049 | 25 | 8 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 4.412 | 35.1 | 2.76 | 2025-06-25 | 121 | 141 | neurological disorders; Parkinson's disease; Alzheimer's disease; glioblastoma; epilepsy; multiple sclerosis; nose-to-brain; blood brain barrier; nanoformulations | BLOOD-BRAIN-BARRIER; LOADED CHITOSAN NANOPARTICLES; NASAL DRUG-DELIVERY; SILVER NANOPARTICLES; MUCOADHESIVE MICROEMULSIONS; MAGNETIC NANOPARTICLES; CEREBROSPINAL-FLUID; PLGA NANOPARTICLES; GOLD NANOPARTICLES; GROWTH-FACTOR | Alzheimer’s disease; Blood brain barrier; Epilepsy; Glioblastoma; Multiple sclerosis; Nanoformulations; Neurological disorders; Nose-to-brain; Parkinson’s disease | Administration, Intranasal; Animals; Blood-Brain Barrier; Central Nervous System Diseases; Drug Administration Routes; Drug Carriers; Drug Compounding; Drug Delivery Systems; Humans; Nanoparticles; Permeability; Theranostic Nanomedicine; drug carrier; nanoparticle; animal; blood brain barrier; central nervous system disease; chemistry; drug administration route; drug delivery system; drug formulation; human; intranasal drug administration; metabolism; permeability; theranostic nanomedicine | English | 2020 | 2020-04-02 | 10.3390/molecules25081929 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Isolation of Isocoumarins and Flavonoids as α-Glucosidase Inhibitors from Agrimonia pilosa L. | Agrimonia pilosa L. (AP) showed potent alpha -glucosidase inhibitory (AGI) activity, but it is uncertain what phytochemicals play a key factor. The phytochemical study of AP based on AGI activity led to the isolation of four isocoumarins; agrimonolide (1), agrimonolide-6-O-beta -d-glucopyranoside (2), desmethylagrimonolide (3), desmethylagrimonolide-6-O-beta -d-glucopyranoside (4), and four flavonoids; luteolin (5), quercetin (6), vitexin (7), and isovitexin (8). The four isocoumarins were isolated as alpha -glucosidase inhibitors for the first time. Isocoumarins, compound 1 (agrimonolide) and 3 (desmethylagrimonolide) showed strong alpha -glucosidase inhibitory activities with IC50 values of 24.2 and 37.4 mu M, respectively. Meanwhile, isocoumarin and flavonoid glycosides showed weak AGI activity. In the kinetic analysis, isocoumarins, compounds 1 and 3 showed non-competitive inhibition, whereas flavonoid, compound 6 showed competitive inhibition. | Park, Mi Jin; Kang, Young-Hwa | Kyungpook Natl Univ, Div Appl Biosci, Coll Agr & Life Sci, Daegu 41566, South Korea | 56319353100; 56423929300 | mj-7311@hanmail.net;youngh@knu.ac.kr; | MOLECULES | MOLECULES | 1420-3049 | 25 | 11 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 4.412 | 35.1 | 0.74 | 2025-06-25 | 19 | 18 | Agrimonia pilosa L; alpha-glucosidase inhibitory activity; isocoumarin; flavonoid; structure-activity relationship; industrial source | ANTIOXIDANT ACTIVITIES; IN-VITRO; EXTRACT; AMYLASE; ROOTS; LEDEB | Agrimonia pilosa L.; Flavonoid; Industrial source; Isocoumarin; Structure-activity relationship; α-glucosidase inhibitory activity | Agrimonia; Drugs, Chinese Herbal; Flavonoids; Glycoside Hydrolase Inhibitors; Glycosides; Hypoglycemic Agents; Inhibitory Concentration 50; Isocoumarins; Kinetics; Magnetic Resonance Spectroscopy; Methanol; Molecular Structure; Phytochemicals; Plant Extracts; Quercetin; Structure-Activity Relationship; agrimonolide; antidiabetic agent; flavonoid; glycosidase inhibitor; glycoside; herbaceous agent; isocoumarin derivative; methanol; phytochemical; plant extract; quercetin; Agrimonia; chemical structure; chemistry; IC50; isolation and purification; kinetics; nuclear magnetic resonance spectroscopy; structure activity relation | English | 2020 | 2020-06 | 10.3390/molecules25112572 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Review | Present and Future of Anti-Glioblastoma Therapies: A Deep Look into Molecular Dependencies/Features | Glioblastoma (GBM) is aggressive malignant tumor residing within the central nervous system. Although the standard treatment options, consisting of surgical resection followed by combined radiochemotherapy, have long been established for patients with GBM, the prognosis is still poor. Despite recent advances in diagnosis, surgical techniques, and therapeutic approaches, the increased patient survival after such interventions is still sub-optimal. The unique characteristics of GBM, including highly infiltrative nature, hard-to-access location (mainly due to the existence of the blood brain barrier), frequent and rapid recurrence, and multiple drug resistance mechanisms, pose challenges to the development of an effective treatment. To overcome current limitations on GBM therapy and devise ideal therapeutic strategies, efforts should focus on an improved molecular understanding of GBM pathogenesis. In this review, we summarize the molecular basis for the development and progression of GBM as well as some emerging therapeutic approaches. | Kim, Hyeon Ji; Kim, Do-Yeon | Kyungpook Natl Univ, Sch Dent, Dept Pharmacol, Daegu 41940, South Korea; Kyungpook Natl Univ, Brain Sci & Engn Inst, Sch Dent, Dept Pharmacol, Daegu 41940, South Korea | Kim, Hee/AAU-6368-2021; Kim, Do-Yeon/AET-3021-2022 | 57216816929; 57203012542 | guswl1634@naver.com;dykim82@knu.ac.kr; | MOLECULES | MOLECULES | 1420-3049 | 25 | 20 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 4.412 | 35.1 | 0.18 | 2025-06-25 | 10 | 10 | glioblastoma; molecular pathogenesis; immunotherapy; drug resistance; personalized therapies | INTEGRATED GENOMIC ANALYSIS; TERT PROMOTER MUTATIONS; CENTRAL-NERVOUS-SYSTEM; TEMOZOLOMIDE RESISTANCE; TUMOR ANGIOGENESIS; RADIATION-THERAPY; GLIOMA PATIENTS; CANCER-THERAPY; GENE; P53 | Drug resistance; Glioblastoma; Immunotherapy; Molecular pathogenesis; Personalized therapies | Brain Neoplasms; Female; Glioblastoma; Humans; Immunotherapy; Male; Prognosis; brain tumor; female; glioblastoma; human; immunotherapy; male; pathology; prognosis | English | 2020 | 2020-10 | 10.3390/molecules25204641 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Pu-erh Tea Extract Treatment Could Be an Efficient Way to Enhance the Yield and Nutritional Value of Soybean Sprout | Soybean sprouts are one of the most inexpensive and nutritious food items that can be easily grown year-round. Several studies have been conducted to increase their yield and nutritional values. This study was carried out to examine the effects of Pu-erh tea extracts on the production and nutrients content of soybean sprouts. Soybean seeds were soaked in 1%, 2%, or 3% (w/v) tea extracts, or tap water, before keeping for sprout cultivation; the sprout samples were named PE-1, PE-2, PE-3, and the control, respectively. The sprout yields were increased by up to 17% in PE-2 and PE-3 than in the control. The vitamin C, total free amino acid, total mineral, total isoflavone, total polyphenol, and flavonoid contents as well as the antioxidant potentials of the tea extract-treated sprouts were higher than those of the control. The results indicated that pre-soaking soybean seeds in 2% Pu-erh tea extracts could offer an easy, inexpensive, and efficient way to improve the yield and nutritional value of soybean sprouts. | Kim, Jeong-Ho; Yoon, Yong-Han; Kim, Il-Doo; Dhungana, Sanjeev Kumar; Shin, Dong-Hyun | Konkuk Univ, Dept Green Technol Convergence, Chungju 27478, South Korea; Kyungpook Natl Univ, Int Inst Res & Dev, Daegu 41566, South Korea; Rural Dev Adm, Natl Inst Crop Sci, Dept Southern Area Crop Sci, Miryang 50424, South Korea; Kyungpook Natl Univ, Sch Appl Biosci, Daegu 41566, South Korea | Kim, Il-Doo/C-1850-2011; Kim, Jeong-Ho/A-7641-2018; Dhungana, Sanjeev Kumar/O-4097-2017 | 57221537227; 56230173600; 56269995600; 56269940800; 7403352903 | hoya1209@kku.ac.kr;yonghan7204@kku.ac.kr;ildookim@hanmail.net;sanjeevdhungana@yahoo.com;dhshin@knu.ac.kr; | MOLECULES | MOLECULES | 1420-3049 | 25 | 17 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 4.412 | 35.1 | 0.41 | 2025-06-25 | 6 | 8 | antioxidant potential; nutrient; Pu-erh tea; soybean sprout; yield | MAX L. MERRILL; ANTIOXIDANT ACTIVITY; PHENOLIC-COMPOUNDS; QUALITY; ISOFLAVONES; GABA; GERMINATION; CALCIUM; GROWTH; ZINC | Antioxidant potential; Nutrient; Pu-erh tea; Soybean sprout; Yield | Antioxidants; Nutritive Value; Plant Extracts; Seedlings; Soybeans; Tea; antioxidant; plant extract; chemistry; growth, development and aging; nutritional value; seedling; soybean; tea | English | 2020 | 2020-09 | 10.3390/molecules25173869 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Review | Radioanalytical Techniques to Quantitatively Assess the Biological Uptake and In Vivo Behavior of Hazardous Substances | Concern about environmental exposure to hazardous substances has grown over the past several decades, because these substances have adverse effects on human health. Methods used to monitor the biological uptake of hazardous substances and their spatiotemporal behavior in vivo must be accurate and reliable. Recent advances in radiolabeling chemistry and radioanalytical methodologies have facilitated the quantitative analysis of toxic substances, and whole-body imaging can be achieved using nuclear imaging instruments. Herein, we review recent literature on the radioanalytical methods used to study the biological distribution, changes in the uptake and accumulation of hazardous substances, including industrial chemicals, nanomaterials, and microorganisms. We begin with an overview of the radioisotopes used to prepare radiotracers for in vivo experiments. We then summarize the results of molecular imaging studies involving radiolabeled toxins and their quantitative assessment. We conclude the review with perspectives on the use of radioanalytical methods for future environmental research. | Lee, Jae Young; Mushtaq, Sajid; Park, Jung Eun; Shin, Hee Soon; Lee, So-Young; Jeon, Jongho | Ajou Univ, Dept Environm & Safety Engn, Suwon 16499, South Korea; Pakistan Inst Engn & Appl Sci, Dept Nucl Engn, Islamabad 45650, Pakistan; Kyungpook Natl Univ, Sch Appl Chem Engn, Dept Appl Chem, Daegu 41566, South Korea; Korea Food Res Inst, Div Funct Food Res, 245 Nongsaengmyeong Ro, Wanju Gun 55365, Jeollabuk Do, South Korea; Univ Sci & Technol, Food Biotechnol Program, Daejeon 34113, South Korea | ; Lee, Jae-Young/GWZ-2558-2022; Lee, YoungMi/JCF-0461-2023 | 57217999869; 57207516170; 57210160353; 55413401300; 57204518100; 35082028100 | jaeylee@ajou.ac.kr;sajidmushtaq@pieas.edu.pk;pje1204@knu.ac.kr;hsshin@kfri.re.kr;sylee09@kfri.re.kr;jeonj@knu.ac.kr; | MOLECULES | MOLECULES | 1420-3049 | 25 | 17 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 4.412 | 35.1 | 0.21 | 2025-06-25 | 10 | 10 | hazardous substances; biodistribution; radiolabeling; in vivo imaging; environmental health | S-35-LABELED PERFLUOROOCTANE SULFONATE; TISSUE DISTRIBUTION; EFFICIENT METHOD; BISPHENOL-A; TRACKING; EXPOSURE; CHALLENGES; STRATEGIES; CHEMICALS; MICE | Biodistribution; Environmental health; Hazardous substances; In vivo imaging; Radiolabeling | Animals; Bacteria; Hazardous Substances; Humans; Nanostructures; Radioisotopes; Technology, Radiologic; Tissue Distribution; nanomaterial; radioisotope; animal; bacterium; chemistry; dangerous goods; human; metabolism; procedures; radiology; tissue distribution | English | 2020 | 2020-09 | 10.3390/molecules25173985 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Structural Basis for Broad Substrate Selectivity of Alcohol Dehydrogenase YjgB from Escherichia coli | In metabolic engineering and synthetic biology fields, there have been efforts to produce variable bioalcohol fuels, such as isobutanol and 2-phenylethanol, in order to meet industrial demands. YjgB is an aldehyde dehydrogenase from Escherichia coli that shows nicotinamide adenine dinucleotide phosphate (NADP)-dependent broad selectivity for aldehyde derivatives with an aromatic ring or small aliphatic chain. This could contribute to the design of industrial synthetic pathways. We determined the crystal structures of YjgB for both its apo-form and NADP-complexed form at resolutions of 1.55 and 2.00 angstrom, respectively, in order to understand the mechanism of broad substrate selectivity. The hydrophobic pocket of the active site and the nicotinamide ring of NADP(H) are both involved in conferring its broad specificity toward aldehyde substrates. In addition, based on docking-simulation data, we inferred that pi-pi stacking between substrates and aromatic side chains might play a crucial role in recognizing substrates. Our structural analysis of YjgB might provide insights into establishing frameworks to understand its broad substrate specificity and develop engineered enzymes for industrial biofuel synthesis. | Giang Thu Nguyen; Kim, Yeon-Gil; Ahn, Jae-Woo; Chang, Jeong Ho | Kyungpook Natl Univ, Dept Biol Educ, 80 Daehak Ro, Daegu 41566, South Korea; Pohang Accelerator Lab, Beamline Sci Div, 127 Jigok Ro, Pohang 37673, Gyoungbuk, South Korea; Pohang Univ Sci & Technol, Postech Biotech Ctr, 77 Cheongam Ro, Pohang 37673, Gyoungbuk, South Korea; Kyungpook Natl Univ, Dept Biomed Convergence Sci & Technol, 80 Daehak Ro, Daegu 41566, South Korea | ; Kim, Jae-hyung/J-8504-2012 | 57211290188; 35781059400; 57191577996; 57203598905 | thugiang1995@gmail.com;ygkim76@postech.ac.kr;jaewooahn@postech.ac.kr;jhcbio@knu.ac.kr; | MOLECULES | MOLECULES | 1420-3049 | 25 | 10 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 4.412 | 35.1 | 0.16 | 2025-06-25 | 9 | 8 | YjgB; aldehyde dehydrogenase; broad specificity; crystal structure; hydrophobic pocket; docking-simulation | FLAVORS; TOOLS; YQHD; MDR | Aldehyde dehydrogenase; Broad specificity; Crystal structure; Docking-simulation; Hydrophobic pocket; YjgB | Alcohol Dehydrogenase; Alcohol Oxidoreductases; Binding Sites; Catalytic Domain; Crystallography, X-Ray; Escherichia coli; Escherichia coli Proteins; Metabolic Engineering; Models, Molecular; Protein Conformation; Substrate Specificity; alcohol dehydrogenase; Escherichia coli protein; YjgB protein, E coli; binding site; chemistry; enzyme active site; enzyme specificity; enzymology; Escherichia coli; genetics; metabolic engineering; molecular model; protein conformation; ultrastructure; X ray crystallography | English | 2020 | 2020-05 | 10.3390/molecules25102404 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Review | Tooth-Supporting Hard Tissue Regeneration Using Biopolymeric Material Fabrication Strategies | The mineralized tissues (alveolar bone and cementum) are the major components of periodontal tissues and play a critical role to anchor periodontal ligament (PDL) to tooth-root surfaces. The integrated multiple tissues could generate biological or physiological responses to transmitted biomechanical forces by mastication or occlusion. However, due to periodontitis or traumatic injuries, affect destruction or progressive damage of periodontal hard tissues including PDL could be affected and consequently lead to tooth loss. Conventional tissue engineering approaches have been developed to regenerate or repair periodontium but, engineered periodontal tissue formation is still challenging because there are still limitations to control spatial compartmentalization for individual tissues and provide optimal 3D constructs for tooth-supporting tissue regeneration and maturation. Here, we present the recently developed strategies to induce osteogenesis and cementogenesis by the fabrication of 3D architectures or the chemical modifications of biopolymeric materials. These techniques in tooth-supporting hard tissue engineering are highly promising to promote the periodontal regeneration and advance the interfacial tissue formation for tissue integrations of PDL fibrous connective tissue bundles (alveolar bone-to-PDL or PDL-to-cementum) for functioning restorations of the periodontal complex. | Kim, Min Guk; Park, Chan Ho | Kyungpook Natl Univ, Grad Sch, Dept Dent Sci, Daegu 41940, South Korea; Kyungpook Natl Univ, Dept Dent Biomat, Sch Dent, Daegu 41940, South Korea; Kyungpook Natl Univ, Inst Biomat Res & Dev, Daegu 41940, South Korea | 57219538160; 55728043300 | minguk.kim@knu.ac.kr;chanho@knu.ac.kr; | MOLECULES | MOLECULES | 1420-3049 | 25 | 20 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2020 | 4.412 | 35.1 | 0.3 | 2025-06-25 | 16 | 17 | biopolymers; alveolar bone; cementum; tissue engineering; regenerative medicine; fabrication; periodontal tissues | GROWTH-FACTOR DELIVERY; ENAMEL MATRIX PROTEINS; PERIODONTAL-LIGAMENT; BONE REGENERATION; EXTRACELLULAR-MATRIX; DRUG-DELIVERY; MECHANICAL-PROPERTIES; CONTROLLED-RELEASE; ALVEOLAR BONE; STEM-CELLS | Alveolar bone; Biopolymers; Cementum; Fabrication; Periodontal tissues; Regenerative medicine; Tissue engineering | Animals; Biopolymers; Humans; Osteogenesis; Periodontal Ligament; Periodontitis; Periodontium; Regeneration; Tissue Engineering; Tooth; Wound Healing; biopolymer; animal; bone development; drug effect; growth, development and aging; human; pathology; periodontal ligament; periodontitis; periodontium; regeneration; tissue engineering; tooth; wound healing | English | 2020 | 2020-10 | 10.3390/molecules25204802 | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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