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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | ○ | Review | Preventive Effect of Pharmaceutical Phytochemicals Targeting the Src Family of Protein Tyrosine Kinases and Aryl Hydrocarbon Receptor on Environmental Stress-Induced Skin Disease | The skin protects our body; however, it is directly exposed to the environment and is stimulated by various external factors. Among the various environmental factors that can threaten skin health, the effects of ultraviolet (UV) and particulate matter (PM) are considered the most notable. Repetitive exposure to ultraviolet and particulate matter can cause chronic skin diseases such as skin inflammation, photoaging, and skin cancer. The abnormal activation of the Src family of protein tyrosine kinases (SFKs) and the aryl hydrocarbon receptor (AhR) in response to UV and/or PM exposure are involved in the development and aggravation of skin diseases. Phytochemicals, chemical compounds of natural plants, exert preventive effects on skin diseases through the regulation of various signaling pathways. Therefore, this review aims to highlight the efficacy of phytochemicals as potential nutraceuticals and pharmaceutical materials for the treatment of skin diseases, primarily by targeting SFK and AhR, and to explore the underlying mechanisms of action. Future studies are essential to validate the clinical potential for the prevention and treatment of skin diseases. | Paik, So Jeong; Kim, Dong Joon; Jung, Sung Keun | Kyungpook Natl Univ, Sch Food Sci & Biotechnol, Daegu 41566, South Korea; Dankook Univ, Coll Med, Dept Microbiol, Cheonan 31116, South Korea; Kyungpook Natl Univ, Res Inst Tailored Food Technol, Daegu 41566, South Korea | ; Jung, SUNG KEUN/AGR-2623-2022; Kim, Dong Joon/KDN-8414-2024 | 58161897900; 57203012616; 35310491400 | sjpaik0916@gmail.com;kjoon95@dankook.ac.kr;kjung04@knu.ac.kr;skjung04@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 6 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0.16 | 2025-06-25 | 2 | 3 | phytochemical; skin disease; prevention; src family of protein tyrosine kinases; aryl hydrocarbon receptor; environmental stress; signaling pathways | INDUCED DNA-DAMAGE; OXIDATIVE STRESS; AH RECEPTOR; SIGNALING PATHWAY; C-SRC; ULTRAVIOLET-RADIATION; PARTICULATE MATTER; MOUSE SKIN; INFLAMMATION; CANCER | aryl hydrocarbon receptor; environmental stress; phytochemical; prevention; signaling pathways; skin disease; src family of protein tyrosine kinases | Humans; Particulate Matter; Pharmaceutical Preparations; Protein-Tyrosine Kinases; Receptors, Aryl Hydrocarbon; Skin Diseases; src-Family Kinases; aromatic hydrocarbon receptor; nutraceutical; phytochemical; protein tyrosine kinase; aromatic hydrocarbon receptor; drug; protein tyrosine kinase; air pollution; cutaneous parameters; dermatitis; drug effect; drug efficacy; drug mechanism; environmental factor; environmental stress; enzyme activity; human; nonhuman; particulate matter; protein structure; protein targeting; Review; skin carcinogenesis; skin disease; ultraviolet irradiation; metabolism; skin disease | English | 2023 | 2023-03 | 10.3390/ijms24065953 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Procyanidin B2 Attenuates Sepsis-Induced Acute Lung Injury via Regulating Hippo/Rho/PI3K/NF-κB Signaling Pathway | Acute lung injury (ALI) is a frequent and challenging aspect of sepsis that currently lacks effective treatments. Procyanidin B2 (PB2) has anti-inflammatory and antioxidant properties. The aim of this study was to determine the effectiveness and mechanism of action of PB2 in treating sepsis-induced ALI using animal experiments. A sepsis-induced ALI mouse model was used by administering lipopolysaccharide (LPS) and then evaluating the levels of inflammatory cytokines and lung injury through measurements of cytokine levels using enzyme-linked immunosorbent assay (ELISA), Western blot and real-time PCR, as well as by the examination of relevant signaling pathways. The animal experiments showed that PB2 protected the lungs from injury caused by LPS and reduced the levels of various inflammatory cytokines in both the serum and lung tissue. Western blot analysis showed that PB2 reduced the expression of TLR4/NF-?B and increased the expression of PI3K/Akt, and also inhibited the Hippo and Rho signaling pathways. The results of the study showed that PB2 helps to treat sepsis-induced ALI by controlling cytokine storms and reducing inflammation by altering the expressions of the TLR4/NF-?B, PI3K/Akt, Hippo and Rho signaling pathways. This research provides a foundation for the further investigation of PB2's mechanism and its potential use in treating sepsis. | Kim, Go Oun; Park, Dong Ho; Bae, Jong-Sup | Kyungpook Natl Univ, Res Inst Pharmaceut Sci, Coll Pharm, Daegu 41566, South Korea; Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Dept Ophthalmol, Daegu 41944, South Korea | Bae, Jong-Sup/AAU-9724-2020 | 57896809800; 36676632900; 16021543200 | rhdns9231@gmail.com;dongho_park@knu.ac.kr;baejs@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 9 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 1.61 | 2025-06-25 | 13 | 12 | procyanidin B2; sepsis; acute lung injury; hippo; rho | TOLL-LIKE RECEPTORS; ACTIVATED PROTEIN-C; PHOSPHOINOSITIDE 3-KINASE; MITOCHONDRIAL DYSFUNCTION; APOPTOSIS; AXIS | acute lung injury; hippo; procyanidin B2; rho; sepsis | Acute Lung Injury; Animals; Cytokines; Lipopolysaccharides; Lung; Mice; NF-kappa B; Phosphatidylinositol 3-Kinases; Proto-Oncogene Proteins c-akt; Sepsis; Signal Transduction; Toll-Like Receptor 4; antiinflammatory agent; beta actin; dexamethasone; eosin; gamma interferon; granulocyte macrophage colony stimulating factor; hematoxylin; immunoglobulin enhancer binding protein; interleukin 1beta; interleukin 5; interleukin 6; long acting thyroid stimulator; messenger RNA; phosphatidylinositol 3 kinase; procyanidin B2; procyanidin derivative; protein kinase B; Rho guanine nucleotide binding protein; Rho kinase; RhoC guanine nucleotide binding protein; toll like receptor 4; tumor necrosis factor; unclassified drug; YAP signaling protein; cytokine; immunoglobulin enhancer binding protein; lipopolysaccharide; phosphatidylinositol 3 kinase; procyanidin B2; protein kinase B; toll like receptor 4; animal experiment; animal model; animal tissue; antiinflammatory activity; Article; controlled study; cytokine storm; drug efficacy; drug mechanism; enzyme linked immunosorbent assay; hippo signaling; inflammation; inflammatory cell; lipopolysaccharide-induced acute lung injury; lipopolysaccharide-induced sepsis; lung parenchyma; male; mouse; mRNA expression level; NF kB signaling; nonhuman; Pi3K/Akt signaling; protein blood level; protein expression; real time polymerase chain reaction; TLR signaling; Western blotting; acute lung injury; animal; complication; lung; metabolism; sepsis; signal transduction | English | 2023 | 2023-04-27 | 10.3390/ijms24097930 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Protaetia Brevitarsis-Derived Protein Hydrolysate Reduces Obesity-Related Colitis Induced by High-Fat Diet in Mice through Anti-Inflammatory Pathways | Ulcerative colitis is an inflammatory bowel disease characterized by inflammation in the mucosal and submucosal layers of the colon. Obesity is closely related to the occurrence and progression of colitis. The most plausible mechanism linking obesity and colitis is an excessive adipogenesis-related inflammatory response, which causes mucosal dysfunction. Obesity and colitis are linked by several etiologic mechanisms, including excessive adipogenesis, lipotoxicity, pro-inflammatory adipokines/cytokines, macrophage polarization, oxidative stress, endoplasmic reticulum (ER) stress, and gut microbiota. These low-grade enteric inflammations cause mucosal layer damage, especially goblet cell dysfunction through mucin 2 (MUC2) misfolding, ultimately leading to colitis. Inhibiting the inflammatory response can be the most effective approach for treating obesity-related colitis. We focused on the anti-inflammatory effects of polyphenols in Protaectia brevitas larvae. The P. brevitas was prepared as a low molecular protein hydrolysate (PHPB) to increase the concentration of anti-inflammatory molecules. In the current study, we investigated the anti-inflammatory effect of PHPB in an obesity-induced colitis mouse model. Compared with the high-fat diet (HFD) group, the group treated with PHPB exhibited reduced body/organ/fat weight, appetite/food intake inhibition, hypolipidemic effect on ectopic fat, and anti-adipogenic mechanism through the AMPK signaling pathway. Furthermore, we observed attenuated expression of PPAR & gamma; and C/EBP & alpha;, inhibition of pro-inflammatory molecules, stimulation of anti-inflammatory molecules, probiotic-like effect against obesogenic gut microbiota, inhibition of macrophage polarization into M1, suppression of oxidative/ER stress, and reduction of Muc2 protein misfolding in colon. These diverse anti-inflammatory responses caused histological and functional recovery of goblet cells, eventually improving colitis. Therefore, our findings suggest that the protein hydrolysate of Protaetia brevitarsis can improve obesity-related colitis through its anti-inflammatory activities. | Kwon, Hyung Jun; Chun, So Young; Lee, Eun Hye; Yoon, BoHyun; Han, Man-Hoon; Chung, Jae-Wook; Ha, Yun-Sok; Lee, Jun Nyung; Kim, Hyun Tae; Kim, Dae Hwan; Kwon, Tae Gyun; Kim, Bum Soo; Lee, Syng-Ook; Jang, Byung Ik | Kyungpook Natl Univ, Sch Med, Dept Surg, Daegu 41566, South Korea; Kyungpook Natl Univ Hosp, Biomed Res Inst, Daegu 41944, South Korea; Kyungpook Natl Univ, Joint Inst Regenerat Med, Daegu 41566, South Korea; Kyungpook Natl Univ, Sch Med, Dept Pathol, Daegu 41566, South Korea; Kyungpook Natl Univ, Sch Med, Dept Urol, Daegu 41566, South Korea; Yeungnam Univ, Med Ctr, Dept Lab Anim Res Support Team, Daegu 42415, South Korea; Keimyung Univ, Dept Food Sci & Technol, Daegu 42601, South Korea; Yeungnam Univ, Sch Med, Dept Internal Med, Daegu 42415, South Korea | ; Kim, Soo-Yeon/ADR-9663-2022; Lee, Jae-Sung/A-5236-2015; Kim, Jihyun/F-6940-2013; Lee, Jun Young/CAI-2335-2022 | 57199757347; 8688166900; 57189661699; 57218931150; 57194067936; 35204798500; 35487226400; 16301364600; 55739531300; 55897992900; 15073765400; 57202817150; 12796984400; 22953491400 | kwonhj95@knu.ac.kr;jeus119@hanmail.net;ljnlover@gmail.com;ikorando5@hanmail.net;tgkwon@knu.ac.kr;urokbs@knu.ac.kr;synglee@kmu.ac.kr;jbi@med.yu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 15 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0.94 | 2025-06-25 | 5 | 7 | colitis; P; brevitarsis; anti-inflammatory response; protein hydrolysate; high-fat diet | ULCERATIVE-COLITIS; CROHNS-DISEASE; INFLAMMATION; STRESS; MICROBIOTA; RISK | anti-inflammatory response; colitis; high-fat diet; P. brevitarsis; protein hydrolysate | Animals; Anti-Inflammatory Agents; Colitis; Diet, High-Fat; Inflammation; Mice; Mice, Inbred C57BL; Obesity; Protein Hydrolysates; adipocytokine; antiinflammatory agent; CCAAT enhancer binding protein alpha; cytokine; hydroxymethylglutaryl coenzyme A reductase kinase; mucin 2; peroxisome proliferator activated receptor gamma; polyphenol; probiotic agent; protein hydrolysate; antiinflammatory agent; protein hydrolysate; adipogenesis; AMPK signaling; animal experiment; animal model; animal tissue; antiinflammatory activity; antioxidant activity; appetite; Article; body fat; body weight; cell function; colitis; colon tissue; controlled study; disease association; drug efficacy; drug mechanism; endoplasmic reticulum stress; epithelium cell; food intake; gene expression profiling; goblet cell; histopathology; hypolipidemic activity; immunohistochemistry; inflammation; insect; intestine flora; intra-abdominal fat; larva; lipid diet; lipid metabolism; lipid storage; lipotoxicity; M1 macrophage; male; mouse; nonhuman; obesity; oxidative stress; pathogenesis; phenotype; polarization; Protaetia brevitarsis; protein expression level; protein misfolding; regulatory mechanism; signal transduction; treatment response; animal; C57BL mouse; colitis; lipid diet; metabolism; obesity | English | 2023 | 2023-08 | 10.3390/ijms241512333 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Protective Effects of Cirsilineol against Lipopolysaccharide-Induced Inflammation; Insights into HO-1, COX-2, and iNOS Modulation | In this study, the potential protective effects of cirsilineol (CSL), a natural compound found in Artemisia vestita, were examined on lipopolysaccharide (LPS)-induced inflammatory responses. CSL was found to have antioxidant, anticancer, and antibacterial properties, and was lethal to many cancer cells. We assessed the effects of CSL on heme oxygenase (HO)-1, cyclooxygenase (COX)-2, and inducible nitric oxide synthase (iNOS) in LPS-activated human umbilical vein endothelial cells (HUVECs). We also examined the effects of CSL on the expression of iNOS, tumor necrosis factor (TNF)-alpha, and interleukin (IL)-1 beta in the pulmonary histological status of LPS-injected mice. The results showed that CSL increased HO-1 production, inhibited luciferase-NF-kappa B interaction, and reduced COX-2/PGE2 and iNOS/NO levels, leading to a decrease in signal transducer and activator of transcription (STAT)-1 phosphorylation. CSL also enhanced the nuclear translocation of Nrf2, elevated the binding activity between Nrf2 and antioxidant response elements (AREs), and reduced IL-1 beta expression in LPS-treated HUVECs. We found that CSL's suppression of iNOS/NO synthesis was restored by inhibiting HO-1 through RNAi. In the animal model, CSL significantly decreased iNOS expression in the pulmonary biostructure, and TNF-ff level in the bronchoalveolar lavage fluid. These findings indicate that CSL has anti-inflammatory properties by controlling iNOS through inhibition of both NF-kappa B expression and p-STAT-1. Therefore, CSL may have potential as a candidate for developing new clinical substances to treat pathological inflammation. | Kim, Go Oun; Park, Dong Ho; Bae, Jong-Sup | Kyungpook Natl Univ, Res Inst Pharmaceut Sci, Coll Pharm, Daegu 41566, South Korea; Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Dept Ophthalmol, Daegu 41944, South Korea | Bae, Jong-Sup/AAU-9724-2020 | 57896809800; 36676632900; 16021543200 | baejs@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 10 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0.67 | 2025-06-25 | 5 | 5 | cirsilineol; endothelium; iNOS; p-STAT-1 | CARBON-MONOXIDE; INHIBITION; RELEASE; CELLS | cirsilineol; endothelium; iNOS; p-STAT-1 | Animals; Cyclooxygenase 2; Endothelial Cells; Heme Oxygenase-1; Humans; Inflammation; Lipopolysaccharides; Mice; NF-E2-Related Factor 2; NF-kappa B; Nitric Oxide; Nitric Oxide Synthase Type II; antiinfective agent; antiinflammatory agent; cirsilineol; cyclooxygenase 2; heme oxygenase 1; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; interleukin 1beta; lipopolysaccharide; luciferase; maslinic acid; prostaglandin E2; STAT1 protein; transcription factor Nrf2; tumor necrosis factor; unclassified drug; cirsilineol; cyclooxygenase 2; heme oxygenase 1; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; lipopolysaccharide; nitric oxide; transcription factor Nrf2; animal cell; animal experiment; animal model; antibacterial activity; antiinflammatory activity; antioxidant responsive element; apoptosis; Artemisia; Artemisia vestita; Article; binding affinity; cell differentiation; cell proliferation; cell viability; controlled study; endothelium; histology; human; human cell; lipopolysaccharide-induced inflammation; lung lavage; lung parenchyma; male; mouse; nonhuman; protein expression; protein expression level; protein phosphorylation; real time polymerase chain reaction; regulatory mechanism; umbilical vein endothelial cell; animal; endothelium cell; genetics; inflammation; metabolism | English | 2023 | 2023-05-10 | 10.3390/ijms24108537 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Quantitative Trait Loci Mapping Identified Candidate Genes Involved in Plant Height Regulation in Rice | Rice plant height is an agricultural trait closely related to biomass, lodging tolerance, and yield. Identifying quantitative trait loci (QTL) regions related to plant height regulation and developing strategies to screen potential candidate genes can improve agricultural traits in rice. In this study, a double haploid population (CNDH), derived by crossing 'Cheongcheong' and 'Nagdong' individuals, was used, and a genetic map was constructed with 222 single-sequence repeat markers. In the RM3482-RM212 region on chromosome 1, qPh1, qPh1-1, qPh1-3, qPh1-5, and qPh1-6 were identified for five consecutive years. The phenotypic variance explained ranged from 9.3% to 13.1%, and the LOD score ranged between 3.6 and 17.6. OsPHq1, a candidate gene related to plant height regulation, was screened in RM3482-RM212. OsPHq1 is an ortholog of gibberellin 20 oxidase 2, and its haplotype was distinguished by nine SNPs. Plants were divided into two groups based on their height, and tall and short plants were distinguished and clustered according to the expression level of OsPHq1. QTLs and candidate genes related to plant height regulation, and thus, biomass regulation, were screened and identified in this study, but the molecular mechanism of the regulation remains poorly known. The information obtained in this study will help develop molecular markers for marker-assisted selection and breeding through rice plant height control. | Park, Jae-Ryoung; Jang, Yoon-Hee; Kim, Eun-Gyeong; Hur, Sang-Sun; Kim, Kyung-Min | Rural Dev Adm, Natl Inst Crop Sci, Crop Breeding Div, Wonju 55365, South Korea; Kyungpook Natl Univ, Coastal Agr Res Inst, Daegu 41566, South Korea; Joongbu Univ, Dept BioFood Sci, Div Hlth & Welf, Geunmsan 32713, South Korea; Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea | ; Kim, Kyung-Min Kim/C-7007-2014 | 57211205505; 57219901992; 57221496070; 36987968400; 34868260300 | icd0192@korea.kr;uniunnie@naver.com;dkqkxk632@naver.com;sshur@joongbu.ac.kr;kkm@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 23 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0.67 | 2025-06-25 | 5 | 5 | breeding; gibberellin; plant height; quantitative trait loci; rice | GIBBERELLIN 20-OXIDASE; QTL; AGRICULTURE; RESISTANCE; STABILITY; AUXIN | breeding; gibberellin; plant height; quantitative trait loci; rice | Chromosome Mapping; Humans; Oryza; Phenotype; Plant Breeding; Quantitative Trait Loci; DNA; amino acid sequence; Article; biomass; chromosome 1; chromosome 7; chromosome 9; cultivar; DNA determination; environmental factor; gene identification; gene mapping; genetic association; genetic distance; genetic regulation; genome analysis; haplotype; heritability; lod score; phenotype; phenotypic variation; phylogenetic tree; plant height; protein interaction; quantitative trait locus; quantitative trait locus mapping; rice; sequence analysis; single nucleotide polymorphism; chromosomal mapping; genetics; human; Oryza; plant breeding | English | 2023 | 2023-12 | 10.3390/ijms242316895 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Rhein Induces Oral Cancer Cell Apoptosis and ROS via Suppresse AKT/mTOR Signaling Pathway In Vitro and In Vivo | Oral cancer remains the leading cause of death worldwide. Rhein is a natural compound extracted from the traditional Chinese herbal medicine rhubarb, which has demonstrated therapeutic effects in various cancers. However, the specific effects of rhein on oral cancer are still unclear. This study aimed to investigate the potential anticancer activity and underlying mechanisms of rhein in oral cancer cells. The antigrowth effect of rhein in oral cancer cells was estimated by cell proliferation, soft agar colony formation, migration, and invasion assay. The cell cycle and apoptosis were detected by flow cytometry. The underlying mechanism of rhein in oral cancer cells was explored by immunoblotting. The in vivo anticancer effect was evaluated by oral cancer xenografts. Rhein significantly inhibited oral cancer cell growth by inducing apoptosis and S-phase cell cycle arrest. Rhein inhibited oral cancer cell migration and invasion through the regulation of epithelial-mesenchymal transition-related proteins. Rhein induced reactive oxygen species (ROS) accumulation in oral cancer cells to inhibit the AKT/mTOR signaling pathway. Rhein exerted anticancer activity in vitro and in vivo by inducing oral cancer cell apoptosis and ROS via the AKT/mTOR signaling pathway in oral cancer. Rhein is a potential therapeutic drug for oral cancer treatment. | Zhang, Haibo; Ma, Lei; Kim, Eungyung; Yi, Junkoo; Huang, Hai; Kim, Hyeonjin; Raza, Muhammad Atif; Park, Sijun; Jang, Soyoung; Kim, Kirim; Kim, Sung-Hyun; Lee, Youngkyun; Kim, Eunkyong; Ryoo, Zae Young; Kim, Myoung Ok | Kyungpook Natl Univ, Dept Anim Sci & Biotechnol, Sangju 37224, South Korea; Kyungpook Natl Univ, Res Ctr Horse Ind, Dept Anim Biotechnol, Sangju 37224, South Korea; Henan Univ Chinese Med, Coll Pharm, Zhengzhou 450046, Peoples R China; Hankyung Natl Univ, Sch Anim Life Convergence Sci, Anseong 17579, South Korea; Kyungpook Natl Univ, Sch Life Sci, BK21 FOUR KNU Creat Biores Grp, Daegu 41566, South Korea; Kyungpook Natl Univ, Dept Dent Hyg, Sangju 37224, South Korea; Korea Polytech Coll, Dept Biomed Anal, Chungnam 34134, South Korea; Kyungpook Natl Univ, Sch Dent, Daegu 41566, South Korea | ; Zhang, Haibo/HLP-9266-2023; Yi, Junkoo/JBR-8507-2023; RYOO, ZAEYOUNG/AAQ-1573-2020 | 57221648126; 57217867673; 57217871658; 56182537200; 57215021952; 57343180400; 58291484800; 54682212300; 57139360300; 35793746200; 59103241900; 36062942200; 55915892100; 16937104900; 8934745900 | ok4325@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 10 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 3.48 | 2025-06-25 | 28 | 29 | apoptosis; autophagy; mTOR; oral cancer; rhein | DOWN-REGULATION; LUNG-CANCER; N-CADHERIN; AUTOPHAGY; CARCINOMA; MIGRATION; KINASES; STRESS; GROWTH | apoptosis; autophagy; mTOR; oral cancer; rhein | Apoptosis; Cell Line, Tumor; Cell Proliferation; Humans; Mouth Neoplasms; Proto-Oncogene Proteins c-akt; Reactive Oxygen Species; Signal Transduction; TOR Serine-Threonine Kinases; caspase 3; cyclin A1; cyclin dependent kinase 2; cyclin E1; Ki 67 antigen; mammalian target of rapamycin; microtubule associated protein 1A 1B light chain 3; nerve cell adhesion molecule; protein Bax; protein bcl 2; protein kinase B; reactive oxygen metabolite; rhein; sequestosome 1; sodium chloride; stress activated protein kinase; unclassified drug; uvomorulin; MTOR protein, human; protein kinase B; reactive oxygen metabolite; rhein; target of rapamycin kinase; Akt/mTOR signaling; animal experiment; animal model; animal tissue; antineoplastic activity; antiproliferative activity; apoptosis; Article; autophagy (cellular); body weight; Ca9-22 cell line; cancer inhibition; cell cycle; cell invasion; cell migration; cell proliferation; colony formation; controlled study; down regulation; epithelial mesenchymal transition; flow cytometry; heart; histology; human; human cell; immunoblotting; immunohistochemistry; in vitro study; in vivo study; kidney; liver; lung; male; metastasis inhibition; mouse; mouth cancer; nonhuman; protein expression level; S phase cell cycle checkpoint; treatment duration; tumor volume; tumor xenograft; upregulation; Western blotting; YD-10B cell line; apoptosis; metabolism; mouth tumor; signal transduction; tumor cell line | English | 2023 | 2023-05-09 | 10.3390/ijms24108507 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | S-Nitrosoglutathione (GSNO)-Mediated Lead Detoxification in Soybean through the Regulation of ROS and Metal-Related Transcripts | Heavy metal toxicity, including lead (Pb) toxicity, is increasing in soils, and heavy metals are considered to be toxic in small amounts. Pb contamination is mainly caused by industrialization (e.g., smelting and mining), agricultural practices (e.g., sewage sludge and pests), and urban practices (e.g., lead paint). An excessive concentration of Pb can seriously damage and threaten crop growth. Furthermore, Pb adversely affects plant growth and development by affecting the photosystem, cell membrane integrity, and excessive production of reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) and superoxide (O-2(-)). Nitric oxide (NO) is produced via enzymatic and non-enzymatic antioxidants to scavenge ROS and lipid peroxidation substrates to protect cells from oxidative damage. Thus, NO improves ion homeostasis and confers resistance to metal stress. In the present study, we investigated the effect of exogenously applied NO and S-nitrosoglutathione in soybean plants Our results demonstrated that exogenously applied NO aids in better growth under lead stress due to its ability in sensing, signaling, and stress tolerance in plants under heavy metal stress along with lead stress. In addition, our results showed that S-nitrosoglutathione (GSNO) has a positive effect on soybean seedling growth under lead-induced toxicity and that NO supplementation helps to reduce chlorophyll maturation and relative water content in leaves and roots following strong bursts under lead stress. GSNO supplementation (200 & mu;M and 100 & mu;M) reduced compaction and approximated the oxidative damage of MDA, proline, and H2O2. Moreover, under plant stress, GSNO application was found to relieve the oxidative damage by reactive oxygen species (ROS) scavenging. Additionally, modulation of NO and phytochelatins (PCS) after prolonged metal reversing GSNO application confirmed detoxification of ROS induced by the toxic metal lead in soybean. In summary, the detoxification of ROS caused by toxic metal concentrations in soybean is confirmed by using NO, PCS, and traditionally sustained concentrations of metal reversing GSNO application. | Methela, Nusrat Jahan; Islam, Mohammad Shafiqul; Lee, Da-Sol; Yun, Byung-Wook; Mun, Bong-Gyu | Kyungpook Natl Univ, Coll Agr & Life Sci, Sch Appl Biosci, Dept Plant Biosci, Daegu 41566, South Korea; Noakhali Sci & Technol Univ, Fac Sci, Dept Agr, Noakhali 3814, Bangladesh | ; Islam, Mohammad/K-8232-2019; Mun, BongGyu/GYD-6010-2022 | 57880065700; 59448717500; 57222624235; 8245123600; 57147241300 | mbg@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 12 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 2.01 | 2025-06-25 | 16 | 16 | nitric oxide; S-nitrosoglutathione (GSNO); reactive oxygen species; metal stress; stress tolerance | STRESS; CHLOROPLASTS; TOLERANCE; MECHANISM; ROOTS | metal stress; nitric oxide; reactive oxygen species; S-nitrosoglutathione (GSNO); stress tolerance | Antioxidants; Heavy Metal Poisoning; Hydrogen Peroxide; Lead; Metals, Heavy; Nitric Oxide; Plants; Reactive Oxygen Species; S-Nitrosoglutathione; Soybeans; catalase; chlorophyll; hydrogen peroxide; lead; nitric oxide; phytochelatin; proline; reactive oxygen metabolite; s nitrosoglutathione; superoxide; antioxidant; heavy metal; hydrogen peroxide; lead; nitric oxide; reactive oxygen metabolite; Article; biomass; chelation; chlorophyll content; controlled study; detoxification; gene expression; genetic transcription; hierarchical clustering; homeostasis; lead poisoning; lipid peroxidation; nonhuman; oxidative stress; phenotype; photosynthesis; plant development; plant growth; plant leaf; plant root; plant stress; signal transduction; soybean; supplementation; water content; heavy metal poisoning; metabolism; plant; soybean | English | 2023 | 2023-06 | 10.3390/ijms24129901 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Side-Chain Immune Oxysterols Induce Neuroinflammation by Activating Microglia | In individuals with Alzheimer's disease, the brain exhibits elevated levels of IL-1 beta and oxygenated cholesterol molecules (oxysterols). This study aimed to investigate the effects of side-chain oxysterols on IL-1 beta expression using HMC3 microglial cells and ApoE-deficient mice. Treatment of HMC3 cells with 25-hydroxycholesterol (25OHChol) and 27-hydroxycholesterol (27OHChol) led to increased IL-1 beta expression at the transcript and protein levels. Additionally, these oxysterols upregulated the surface expression of MHC II, a marker of activated microglia. Immunohistochemistry performed on the mice showed increased microglial expression of IL-1 beta and MHC II when fed a high-cholesterol diet. However, cholesterol and 24s-hydroxycholesterol did not increase IL-1 beta transcript levels or MHC II expression. The extent of IL-1 beta increase induced by 25OHChol and 27OHChol was comparable to that caused by oligomeric beta-amyloid, and the IL-1 beta expression induced by the oxysterols was not impaired by polymyxin B, which inhibited lipopolysaccharide-induced IL-1 beta expression. Both oxysterols enhanced the phosphorylation of Akt, ERK, and Src, and inhibition of these kinase pathways with pharmacological inhibitors suppressed the expression of IL-1 beta and MHC II. The pharmacological agents chlorpromazine and cyclosporin A also impaired the oxysterol-induced expression of IL-1 beta and upregulation of MHC II. Overall, these findings suggest that dysregulated cholesterol metabolism leading to elevated levels of side-chain oxysterols, such as 25OHChol and 27OHChol, can activate microglia to secrete IL-1 beta through a mechanism amenable to pharmacologic intervention. The activation of microglia and subsequent neuroinflammation elicited by the immune oxysterols can contribute to the development of neurodegenerative diseases. | Son, Yonghae; Yeo, In-Jun; Hong, Jin-Tae; Eo, Seong-Kug; Lee, Dongjun; Kim, Koanhoi | Pusan Natl Univ, Sch Med, Dept Pharmacol, Yangsan 50612, Gyeongnam, South Korea; Kyungpook Natl Univ, Coll Pharm, 80 Dahak Ro, Daegu 41566, South Korea; Chungbuk Natl Univ, Coll Pharm, Med Res Ctr, Osongsaengmyeong 1 Ro,194-21,Osong eup, Cheongju 28160, South Korea; Jeonbuk Natl Univ, Biosafety Res Inst, Coll Vet Med, Iksan 54596, Jeonbuk, South Korea; Pusan Natl Univ, Dept Convergence Med, Sch Med, Yangsan 50612, South Korea | ; Son, Yonghae/R-6194-2019; Lee, Dongjun/ABF-4381-2021 | 7102761595; 57201941942; 55657116500; 6603701619; 56133551800; 35519698800 | squall0211@hanmail.net;section18@naver.com;jinthong@chungbuk.ac.kr;vetvirus@chonbuk.ac.kr;lee.dongjun@pusan.ac.kr;koanhoi@pusan.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 20 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 1.07 | 2025-06-25 | 8 | 8 | immune oxysterol; interleukin-1 beta; microglia; neuroinflammation | HIGH CHOLESTEROL DIET; E-KNOCKOUT MICE; CYCLOSPORINE-A; ALZHEIMERS-DISEASE; BRAIN CHOLESTEROL; MONOCYTIC CELLS; APOE; LIFE; 27-HYDROXYCHOLESTEROL; 25-HYDROXYCHOLESTEROL | immune oxysterol; interleukin-1β; microglia; neuroinflammation | Animals; Brain; Macrophages; Mice; Microglia; Neuroinflammatory Diseases; Oxysterols; 25 hydroxycholesterol; 26 hydroxycholesterol; amyloid beta protein; apolipoprotein E; chlorpromazine; cyclosporine; interleukin 1beta; lipopolysaccharide; major histocompatibility antigen class 2; mitogen activated protein kinase; mitogen activated protein kinase 1; oxysterol; polymyxin B; protein kinase B; protein tyrosine kinase; animal cell; animal experiment; animal model; animal tissue; Article; cell activation; cholesterol diet; controlled study; enzyme inhibition; enzyme phosphorylation; human; human cell; immunohistochemistry; male; microglia; mouse; nervous system inflammation; nonhuman; protein expression; upregulation; animal; brain; macrophage; metabolism; nervous system inflammation | English | 2023 | 2023-10 | 10.3390/ijms242015288 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Silicon-Induced Morphological, Biochemical and Molecular Regulation in Phoenix dactylifera L. under Low-Temperature Stress | Climate changes abruptly affect optimum growth temperatures, leading to a negative influence on plant physiology and productivity. The present study aimed to investigate the extent of low-temperature stress effects on date palm growth and physiological indicators under the exogenous application of silicon (Si). Date palm seedlings were treated with Si (1.0 mM) and exposed to different temperature regimes (5, 15, and 30 degrees C). It was observed that the application of Si markedly improved fresh and dry biomass, photosynthetic pigments (chlorophyll and carotenoids), plant morphology, and relative water content by ameliorating low-temperature-induced oxidative stress. Low-temperature stress (5 and 15 degrees C), led to a substantial upregulation of ABA-signaling-related genes (NCED-1 and PyL-4) in non Si treated plants, while Si treated plants revealed an antagonistic trend. However, jasmonic acid and salicylic acid accumulation were markedly elevated in Si treated plants under stress conditions (5 and 15 degrees C) in comparison with non Si treated plants. Interestingly, the upregulation of low temperature stress related plant plasma membrane ATPase (PPMA3 and PPMA4) and short-chain dehydrogenases/reductases (SDR), responsible for cellular physiology, stomatal conductance and nutrient translocation under silicon applications, was observed in Si plants under stress conditions in comparison with non Si treated plants. Furthermore, a significant expression of LSi-2 was detected in Si plants under stress, leading to the significant accumulation of Si in roots and shoots. In contrast, non Si plants demonstrated a low expression of LSi-2 under stress conditions, and thereby, reduced level of Si accumulation were observed. Less accumulation of oxidative stress was evident from the expression of superoxide dismutase (SOD) and catalase (CAT). Additionally, Si plants revealed a significant exudation of organic acids (succinic acid and citric acid) and nutrient accumulation (K and Mg) in roots and shoots. Furthermore, the application of Si led to substantial upregulation of the low temperature stress related soybean cold regulated gene (SRC-2) and ICE-1 (inducer of CBF expression 1), involved in the expression of CBF/DREB (C-repeat binding factor/dehydration responsive element binding factor) gene family under stress conditions in comparison with non Si plants. The current research findings are crucial for exploring the impact on morpho-physio-biochemical attributes of date palms under low temperature and Si supplementation, which may provide an efficient strategy for growing plants in low-temperature fields. | Bilal, Saqib; Khan, Taimoor; Asaf, Sajjad; Khan, Nasir Ali; Jan, Syed Saad; Imran, Muhammad; Al-Rawahi, Ahmed; Khan, Abdul Latif; Lee, In-Jung; Al-Harrasi, Ahmed | Univ Nizwa, Nat & Med Sci Res Ctr, Nizwa 616, Oman; Univ Warwick, Sch Life Sci, Coventry CV4 7AL, England; Texas Tech Univ, Inst Genom Crop Abiot Stress Tolerance, Dept Plant & Soil Sci, Lubbock, TX 79409 USA; Kyungpook Natl Univ, Coll Agr & Life Sci, Sch Appl Biosci, Div Plant Biosci, 80 Dahak Ro, Daegu 41566, South Korea; Univ Houston, Dept Engn Technol, Sugar Land, TX 77479 USA | ; Khan, Abdul/B-1005-2010; Imran, Muhammad/AFL-6590-2022; Khan, Abdul/H-5910-2011; Ul-Hamid, Anwar/B-7297-2015; Asaf, Sajjad/ABA-3647-2021; Lee, In-Jung/GLS-0432-2022 | 57031617400; 57886884600; 56595059900; 57320375300; 58137940300; 58282433800; 7801308442; 26639372800; 16425830900; 6506093146 | ijlee@knu.ac.kr;aharrasi@unizwa.edu.om; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 7 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0.94 | 2025-06-25 | 7 | 7 | oxidative stress; plant plasma membrane ATPase; salicylic acid; jasmonic acid; inducer of CBF expression 1 (ICE-1); lipid peroxidation; catalase; low silicon gene | COLD STRESS; ARABIDOPSIS-THALIANA; MEDIATED ALLEVIATION; SIGNAL-TRANSDUCTION; LIPID-PEROXIDATION; TOLERANCE; GROWTH; MECHANISMS; RESISTANCE; RESPONSES | catalase; inducer of CBF expression 1 (ICE-1); jasmonic acid; lipid peroxidation; low silicon gene; oxidative stress; plant plasma membrane ATPase; salicylic acid | Antioxidants; Oxidative Stress; Phoeniceae; Silicon; Temperature; abscisic acid; adenosine triphosphatase; C repeat binding factor dehydration responsive element binding factor; carboxylic acid; carotenoid; catalase; chlorophyll; citric acid; inducer of CBF expression 1; jasmonic acid; magnesium; phytohormone; pigment; plant protein; potassium; salicylic acid; short chain dehydrogenase/reductase; silicon; succinic acid; superoxide dismutase; unclassified drug; antioxidant; silicon; abiotic stress; Article; bioaccumulation; biochemistry; biomass; cell function; cell membrane; cold stress; controlled study; date (fruit); DNA responsive element; dry mass; elemental analysis; gene expression level; lipid peroxidation; low temperature; LSi 2 gene; molecular biology; multigene family; NCED 1 gene; nonhuman; nutrient; oxidative stress; photosynthesis; plant gene; plant growth; plant nutrient; plant root; plant structures; PPMA3 gene; PPMA4 gene; protein expression; PyL 4 gene; seedling; shoot; signal transduction; soybean; SRC 2 gene; stomatal conductance; temperature stress; upregulation; water content; genetics; metabolism; Phoenix (plant); temperature | English | 2023 | 2023-04 | 10.3390/ijms24076036 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Review | Small Molecule Inhibitors for Unc-51-like Autophagy-Activating Kinase Targeting Autophagy in Cancer | Autophagy is a cellular process that removes damaged components of cells and recycles them as biochemical building blocks. Autophagy can also be induced to protect cells in response to intra- and extracellular stresses, including damage to cellular components, nutrient deprivation, hypoxia, and pathogenic invasion. Dysregulation of autophagy has been attributed to various diseases. In particular, autophagy protects cancer cells by supporting tumor cell survival and the development of drug resistance. Understanding the pathophysiological mechanisms of autophagy in cancer has stimulated the research on discovery and development of specific inhibitors targeting various stages of autophagy. In recent years, Unc-51-like autophagy-activating kinase (ULK) inhibitors have become an attractive strategy to treat cancer. This review summarizes recent discoveries and developments in small-molecule ULK inhibitors and their potential as anticancer agents. We focused on structural features, interactions with binding sites, and biological effects of these inhibitors. Overall, this review will provide guidance for using ULK inhibitors as chemical probes for autophagy in various cancers and developing improved ULK inhibitors that would enhance therapeutic benefits in the clinic. | Karmacharya, Ujjwala; Jung, Jong-Wha | Kyungpook Natl Univ, Res Inst Pharmaceut Sci, Coll Pharm, Daegu 41566, South Korea; Kyungpook Natl Univ, Vessel Organ Interact Res Ctr, Daegu 41566, South Korea | 57191042671; 26032128600 | jungj@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 2 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0.9 | 2025-06-25 | 16 | 17 | Unc-51-like autophagy-activating kinase; kinase inhibitor; autophagy; cancer | ULK1; CELL; POTENT; IDENTIFICATION; DISCOVERY; APOPTOSIS; INSIGHTS; COMPLEX; EXERTS | autophagy; cancer; kinase inhibitor; Unc-51-like autophagy-activating kinase | Autophagy; Autophagy-Related Protein-1 Homolog; Humans; Neoplasms; Protein Serine-Threonine Kinases; antineoplastic agent; autophagy related protein; azd 8055; cisplatin; daunorubicin; doxorubicin; gw 406108x; gw 837331x; hesperidin; mrt 67307; mrt 68921; pf 3814735; protein serine threonine kinase inhibitor; sbi 0206965; sbp 7455; serine threonine protein kinase ULK1; serine threonine protein kinase ULK2; sr 20295; u 2; ulk 101; Unc 51 like autophagy activating kinase inhibitor; unclassified drug; xst 14; serine threonine protein kinase ULK1; antineoplastic activity; autophagy (cellular); binding site; biological activity; cancer cell line; cancer inhibition; cancer resistance; combination drug therapy; drug design; drug selectivity; drug structure; human; IC50; ligand binding; malignant neoplasm; pathophysiology; Review; survival; tumor cell; autophagy; metabolism; neoplasm | English | 2023 | 2023-01 | 10.3390/ijms24020953 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Structural Characteristics and Properties of Cocoon and Regenerated Silk Fibroin from Different Silkworm Strains | Silk has attracted the attention of researchers as a biomedical and cosmetic material because of its good biocompatibility and cytocompatibility. Silk is produced from the cocoons of silkworms, which have various strains. In this study, silkworm cocoons and silk fibroins (SFs) were obtained from ten silkworm strains, and their structural characteristics and properties were examined. The morphological structure of the cocoons depended on the silkworm strains. The degumming ratio of silk ranged from 22.8% to 28% depending on the silkworm strains. The highest and lowest solution viscosities of SF were shown by 9671 and 9153, respectively, showing a 12-fold difference. The silkworm strains of 9671, KJ5, and I-NOVI showed a two-fold higher work of ruptures for the regenerated SF film than 181 and 2203, indicating that the silkworm strains considerably influence the mechanical properties of the regenerated SF film. Regardless of the silkworm strain, all silkworm cocoons showed good cell viability, making them suitable candidates for advanced functional biomaterials. | Kim, Yeon Jin; Kim, Seong Wan; Kim, Kee Young; Ki, Chang Seok; Um, In Chul | Kyungpook Natl Univ, Dept Biofibers & Biomat Sci, Daegu 41566, South Korea; Natl Inst Agr Sci, RDA, Wonju 55365, South Korea; Seoul Natl Univ, Dept Agr Forestry & Bioresources, Seoul 08826, South Korea; Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul 08826, South Korea | ; Ki, Chang-Seok/G-7559-2014; Kim, Yeon Jin/J-7844-2019 | 58140454700; 55890317600; 7409321947; 56800160200; 7006725707 | icum@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 5 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0.67 | 2025-06-25 | 3 | 5 | silkworm strain; cocoon; silk fibroin; degumming; structural characteristics; mechanical properties | ELECTRO-SPINNING PERFORMANCE; MOLECULAR-WEIGHT; GELATION BEHAVIOR; SERICIN; MEMBRANE; WET; CRYSTALLINITY; VARIETY; BIODEGRADATION; SOLVENT | cocoon; degumming; mechanical properties; silk fibroin; silkworm strain; structural characteristics | Animals; Biocompatible Materials; Bombyx; Fibroins; Silk; Viscosity; silk fibroin; biomaterial; fibroin; silk; animal experiment; Article; Bombyx mori; cell viability; cocoon; controlled study; hatchability; hydrophilicity; nonhuman; viscosity; animal; Bombyx; chemistry | English | 2023 | 2023-03 | 10.3390/ijms24054965 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Synergistic Effect of Melatonin and Lysinibacillus fusiformis L. (PLT16) to Mitigate Drought Stress via Regulation of Hormonal, Antioxidants System, and Physio-Molecular Responses in Soybean Plants | Drought is one of the most detrimental factors that causes significant effects on crop development and yield. However, the negative effects of drought stress may be alleviated with the aid of exogenous melatonin (MET) and the use of plant-growth-promoting bacteria (PGPB). The present investigation aimed to validate the effects of co-inoculation of MET and Lysinibacillus fusiformis on hormonal, antioxidant, and physio-molecular regulation in soybean plants to reduce the effects of drought stress. Therefore, ten randomly selected isolates were subjected to various plant-growth-promoting rhizobacteria (PGPR) traits and a polyethylene-glycol (PEG)-resistance test. Among these, PLT16 tested positive for the production of exopolysaccharide (EPS), siderophore, and indole-3-acetic acid (IAA), along with higher PEG tolerance, in vitro IAA, and organic-acid production. Therefore, PLT16 was further used in combination with MET to visualize the role in drought-stress mitigation in soybean plant. Furthermore, drought stress significantly damages photosynthesis, enhances ROS production, and reduces water stats, hormonal signaling and antioxidant enzymes, and plant growth and development. However, the co-application of MET and PLT16 enhanced plant growth and development and improved photosynthesis pigments (chlorophyll a and b and carotenoids) under both normal conditions and drought stress. This may be because hydrogen-peroxide (H2O2), superoxide-anion (O2-), and malondialdehyde (MDA) levels were reduced and antioxidant activities were enhanced to maintain redox homeostasis and reduce the abscisic-acid (ABA) level and its biosynthesis gene NCED3 while improving the synthesis of jasmonic acid (JA) and salicylic acid (SA) to mitigate drought stress and balance the stomata activity to maintain the relative water states. This may be possible due to a significant increase in endo-melatonin content, regulation of organic acids, and enhancement of nutrient uptake (calcium, potassium, and magnesium) by co-inoculated PLT16 and MET under normal conditions and drought stress. In addition, co-inoculated PLT16 and MET modulated the relative expression of DREB2 and TFs bZIP while enhancing the expression level of ERD1 under drought stress. In conclusion, the current study found that the combined application of melatonin and Lysinibacillus fusiformis inoculation increased plant growth and could be used to regulate plant function during drought stress as an eco-friendly and low-cost approach. | Imran, Muhammad; Mpovo, Clems Luzolo; Khan, Muhammad Aaqil; Shaffique, Shifa; Ninson, Daniel; Bilal, Saqib; Khan, Murtaza; Kwon, Eun-Hae; Kang, Sang-Mo; Yun, Byung-Wook; Lee, In-Jung | Rural Dev Adm, Natl Inst Agr Sci, Biosafety Div, Jeonju 54874, South Korea; Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea; Qurtuba Univ Sci & Informat Technol, Dept Chem & Life Sci, Peshawar 24830, Pakistan; Univ Nizwa, Nat & Med Sci Res Ctr, Nizwa 616, Oman; Yeungnam Univ, Dept Hort & Life Sci, Gyongsan 38541, South Korea | Imran, Muhammad/AFL-6590-2022; Lee, In-Jung/GLS-0432-2022; Kang, Sang-Mo/MBG-7823-2025; Khan, Muhammad/ABB-9797-2021; shaffique, shifa/KUC-7102-2024 | 58282433800; 58291523700; 57188585606; 57203898867; 58291549000; 57031617400; 57207990116; 57224398710; 56189696900; 8245123600; 16425830900 | m.imran02@yahoo.com;ijlee@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 10 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 3.75 | 2025-06-25 | 27 | 28 | melatonin; plant-growth-promoting rhizobacteria (PGPR); soybean; drought; antioxidants; phytohormones | SPHINGOMONAS SP LK11; OXIDATIVE STRESS; ABSCISIC-ACID; ORGANIC-ACIDS; WATER-STRESS; TOLERANCE; GENE; ARABIDOPSIS; BACTERIA; GROWTH | antioxidants; drought; melatonin; phytohormones; plant-growth-promoting rhizobacteria (PGPR); soybean | Antioxidants; Chlorophyll A; Droughts; Hydrogen Peroxide; Lysinibacillus fusiformis; Melatonin; Soybeans; Stress, Physiological; Water; abscisic acid; antioxidant; basic leucine zipper transcription factor; calcium; carboxylic acid; carotenoid; chlorophyll a; chlorophyll b; exopolysaccharide; hydrogen peroxide; indoleacetic acid; jasmonic acid; melatonin; phytohormone; pigment; reactive oxygen metabolite; siderophore; antioxidant; chlorophyll a; hydrogen peroxide; melatonin; water; antioxidant activity; Article; biosynthesis; controlled study; drought stress; gene expression; homeostasis; hormonal regulation; in vitro study; Lysinibacillus; Lysinibacillus fusiformis; nonhuman; nutrient uptake; oxidation reduction reaction; photosynthesis; plant development; plant growth; plant growth-promoting bacterium; plant response; protein expression; signal transduction; soybean; synergistic effect; drought; genetics; physiological stress; soybean | English | 2023 | 2023-05-09 | 10.3390/ijms24108489 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | The Synergistic Action of Metformin and Glycyrrhiza uralensis Fischer Extract Alleviates Metabolic Disorders in Mice with Diet-Induced Obesity | Metformin, an antidiabetic drug, and Glycyrrhiza uralensis Fischer (GU), an oriental medicinal herb, have been reported to exert anti-obesity effects. This study investigated the synergistic action of metformin and GU in improving diet-induced obesity. Mice were fed a normal diet, a high-fat diet (HFD), or HFD + 0.015% GU water extract for 8 weeks. The HFD and GU groups were then randomly divided into two groups and fed the following diets for the next 8 weeks: HFD with 50 mg/kg metformin (HFDM) and GU with 50 mg/kg metformin (GUM). GUM prevented hepatic steatosis and adiposity by suppressing expression of mRNAs and enzyme activities related to lipogenesis in the liver and upregulating the expression of adipocyte mRNAs associated with fatty acid oxidation and lipolysis, and as a result, improved dyslipidemia. Moreover, GUM improved glucose homeostasis by inducing glucose uptake in tissues and upregulating mRNA expressions associated with glycolysis in the liver and muscle through AMP-activated protein kinase activation. GUM also improved inflammation by increasing antioxidant activity in the liver and erythrocytes and decreasing inflammatory cytokine productions. Here, we demonstrate that GU and metformin exert synergistic action in the prevention of obesity and its complications. | Hong, Min-Kyeong; Han, Youngji; Park, Hae-Jin; Shin, Mi-Rae; Roh, Seong-Soo; Kwon, Eun-Young | Kyungpook Natl Univ, Dept Food Sci & Nutr, 1370 San Kyuk Dong Puk Ku, Daegu 41566, South Korea; Kyungpook Natl Univ, Ctr Food & Nutr Genom Res, 1370 San Kyuk Dong Puk Ku, Daegu 41566, South Korea; Kyungpook Natl Univ, Ctr Beautiful Aging, 1370 San Kyuk Dong Puk Ku, Daegu 41566, South Korea; Med Innovat Complex, Raydel Res Inst, Daegu 41061, South Korea; Daegu Haany Univ, Bio Convergence Testing Ctr, 1 Haanydaero, Gyongsan 38610, South Korea; Daegu Haany Univ, Coll Korean Med, Dept Herbol, 64 Gil,25 Suseongro, Daegu 42158, South Korea | 58026993100; 57206914262; 7601569369; 57040413200; 12752302700; 15765422500 | ddede@dhu.ac.kr;eykwon@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 2 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 1.2 | 2025-06-25 | 7 | 9 | metformin; type 2 diabetes mellitus; obesity; Glycyrrhiza uralensis Fischer; combined treatment | HEPATIC GLUCONEOGENESIS; OXIDATIVE STRESS; MECHANISMS; LICORICE; DISRUPTION; COMPLEX; CELLS | combined treatment; Glycyrrhiza uralensis Fischer; metformin; obesity; type 2 diabetes mellitus | Animals; Diet, High-Fat; Glycyrrhiza uralensis; Liver; Metabolic Diseases; Metformin; Mice; Mice, Inbred C57BL; Obesity; adiponectin; cholesterol; Glycyrrhiza uralensis extract; high density lipoprotein cholesterol; hydroxymethylglutaryl coenzyme A reductase kinase; interleukin 1beta; interleukin 6; leptin; messenger RNA; metformin; monocyte chemotactic protein 1; resistin; triacylglycerol; tumor necrosis factor; metformin; animal experiment; animal model; animal tissue; Article; cholesterol blood level; controlled study; diet-induced obesity; drug potentiation; dyslipidemia; enzyme activation; enzyme activity; fasting blood glucose level; fatty acid blood level; fatty acid level; fatty acid oxidation; fatty liver; gene expression; glucose homeostasis; glucose transport; glycolysis; lipid fingerprinting; lipogenesis; lipolysis; liver weight; male; metabolic disorder; mouse; muscle; nonhuman; triacylglycerol blood level; upregulation; adverse event; animal; C57BL mouse; Glycyrrhiza uralensis; lipid diet; liver; metabolic disorder; metabolism; obesity | English | 2023 | 2023-01 | 10.3390/ijms24020936 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Therapeutic Effects of Cornuside on Particulate Matter-Induced Lung Injury | Particulate matter (PM) is a mixture comprising both organic and inorganic particles, both of which are hazardous to health. The inhalation of airborne PM with a diameter of <= 2.5 mu m (PM2.5) can cause considerable lung damage. Cornuside (CN), a natural bisiridoid glucoside derived from the fruit of Cornus officinalis Sieb, exerts protective properties against tissue damage via controlling the immunological response and reducing inflammation. However, information regarding the therapeutic potential of CN in patients with PM2.5-induced lung injury is limited. Thus, herein, we examined the protective properties of CN against PM2.5-induced lung damage. Mice were categorized into eight groups (n = 10): a mock control group, a CN control group (0.8 mg/kg mouse body weight), four PM2.5+CN groups (0.2, 0.4, 0.6, and 0.8 mg/kg mouse body weight), and a PM2.5+CN group (0.2, 0.4, 0.6, and 0.8 mg/kg mouse body weight). The mice were administered with CN 30 min following intratracheal tail vein injection of PM2.5. In mice exposed to PM2.5, different parameters including changes in lung tissue wet/dry (W/D) lung weight ratio, total protein/total cell ratio, lymphocyte counts, inflammatory cytokine levels in the bronchoalveolar lavage fluid (BALF), vascular permeability, and histology were examined. Our findings revealed that CN reduced lung damage, the W/D weight ratio, and hyperpermeability caused by PM2.5. Moreover, CN reduced the plasma levels of inflammatory cytokines produced because of PM2.5 exposure, such as tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, and nitric oxide, as well as the total protein concentration in the BALF, and successfully attenuated PM2.5-associated lymphocytosis. In addition, CN substantially reduced the expression levels of Toll-like receptors 4 (TLR4), MyD88, and autophagy-related proteins LC3 II and Beclin 1, and increased protein phosphorylation of the mammalian target of rapamycin (mTOR). Thus, the anti-inflammatory property of CN renders it a potential therapeutic agent for treating PM2.5-induced lung injury by controlling the TLR4-MyD88 and mTOR-autophagy pathways. | Kim, Go Oun; Park, Eui Kyun; Park, Dong Ho; Song, Gyu Yong; Bae, Jong-Sup | Kyungpook Natl Univ, Res Inst Pharmaceut Sci, Coll Pharm, Daegu 41566, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Pathol & Regenerat Med, Daegu 41940, South Korea; Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Dept Ophthalmol, Daegu 41944, South Korea; Chungnam Natl Univ, Coll Pharm, 99 Daehak ro, Daejon 34134, South Korea | Bae, Jong-Sup/AAU-9724-2020 | 57896809800; 37071072400; 36676632900; 7402253074; 16021543200 | gysong@cnu.ac.kr;baejs@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 5 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0.27 | 2025-06-25 | 1 | 2 | cornuside; particulate matter; lung injury; TLR4-mTOR-autophagy pathway | NF-KAPPA-B; ACTIVATION; PM2.5; METABOLISM; AUTOPHAGY; GROWTH; CANCER; MTOR | cornuside; lung injury; particulate matter; TLR4–mTOR–autophagy pathway | Adaptor Proteins, Signal Transducing; Animals; Cytokines; Glucosides; Lung; Lung Injury; Mammals; Mice; Myeloid Differentiation Factor 88; Particulate Matter; Toll-Like Receptor 4; TOR Serine-Threonine Kinases; Tumor Necrosis Factor-alpha; 2 morpholino 8 phenylchromone; autophagy related protein; beclin 1; Cornus officinalis extract; cornuside; cytokine; dexamethasone; glucoside; interleukin 10; interleukin 1beta; isoflurane; mammalian target of rapamycin; mitogen activated protein kinase p38; myeloid differentiation factor 88; myeloperoxidase; nitric oxide; protein; small interfering RNA; toll like receptor; toll like receptor 4; tumor necrosis factor; unclassified drug; cornuside; cytokine; glucoside; myeloid differentiation factor 88; signal transducing adaptor protein; target of rapamycin kinase; toll like receptor 4; tumor necrosis factor; adult; air pollutant; animal experiment; animal model; animal tissue; antiinflammatory activity; Article; autophagy (cellular); blood vessel permeability; body weight; bronchoalveolar lavage fluid; cell count; controlled study; Cornus officinalis; cytokine production; dry weight; gene expression; health hazard; histopathology; immune response; inflammation; lung development; lung edema; lung injury; lung parenchyma; lung toxicity; lung weight; lymphocyte count; lymphocytosis; male; MAPK signaling; mouse; mTOR signaling; MVEC cell line; nonhuman; particle size; particulate matter; particulate matter 2.5; particulate matter exposure; PM2.5 exposure; protein blood level; protein expression; protein phosphorylation; respiratory tract disease; signal transduction; tail vein; therapy effect; TLR signaling; Western blotting; adverse event; animal; lung; lung injury; mammal; metabolism; particulate matter; pathology | English | 2023 | 2023-03 | 10.3390/ijms24054979 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Toxicity and Biodistribution of Fragmented Polypropylene Microplastics in ICR Mice | Currently, polypropylene (PP) is used in various products, thus leading to high daily exposure in humans. Thus, it is necessary to evaluate the toxicological effects, biodistribution, and accumulation of PP microplastics in the human body. In this study, administration of two particle sizes of PP microplastics (approximately 5 and 10-50 mu m) did not lead to any significant changes in several toxicological evaluation parameters, including body weight and pathological examination, compared with the control group in ICR mice. Therefore, the approximate lethal dose and no-observed-adverseeffect level of PP microplastics in ICR mice were established as >= 2000 mg/kg. Furthermore, we manufactured cyanine 5.5 carboxylic acid (Cy5.5-COOH)-labeled fragmented PP microplastics to monitor real-time in vivo biodistribution. After oral administration of the Cy5.5-COOH-labeled microplastics to the mice, most of the PP microplastics were detected in the gastrointestinal tract and observed to be out of the body after 24 h in IVIS Spectrum CT. Therefore, this study provides a new insight into the short-term toxicity, distribution, and accumulation of PP microplastics in mammals. | Lee, Sijoon; Kim, Dongseon; Kang, Kyung-Ku; Sung, Soo-Eun; Choi, Joo-Hee; Sung, Minkyoung; Shin, Chang-Hoon; Jeon, Eunyoung; Kim, Dongkyu; Kim, Dongmin; Lee, Sunjong; Kim, Hee-Kyung; Kim, Kilsoo | Daegu Gyeongbuk Med Innovat Fdn, Preclin Res Ctr, Daegu 41061, South Korea; Kyungpook Natl Univ, Dept Med & Biol Engn, 80 Dahakro, Daegu 41566, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Pharmacol, 80 Dahakro, Daegu 41566, South Korea; Korea Inst Ind Technol, Cheonan 31056, South Korea; Kyungpook Natl Univ, Coll Vet Med, 80 Dahakro, Daegu 41566, South Korea | Lee, Sijoon/LIC-1291-2024 | 57219164019; 58291623700; 57215079376; 56051194000; 55882556800; 57256838000; 58291543500; 58291676300; 57779034700; 58291492600; 57129404900; 56014729400; 35272034300 | sjlee1013@kmedihub.re.kr;kds0433@kmedihub.re.kr;kangkk@kmedihub.re.kr;sesung@kmedihub.re.kr;cjh522@kmedihub.re.kr;tjdalsrud27@kmedihub.re.kr;shinchoon@kmedihub.re.kr;duvet111@kmedihub.re.kr;dgkim728@kmedihub.re.kr;sunjong1774@kitech.re.kr;hkkim@kmedihub.re.kr;kskim728@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 10 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 2.27 | 2025-06-25 | 19 | 17 | polypropylene; microplastics; toxicity; biodistribution; Cy5.5-COOH-labeled | POLYSTYRENE MICROPLASTICS | biodistribution; Cy5.5-COOH-labeled; microplastics; polypropylene; toxicity | Animals; Humans; Mammals; Mice; Mice, Inbred ICR; Microplastics; Plastics; Polypropylenes; Tissue Distribution; Water Pollutants, Chemical; cyanine dye 5; microplastic; polypropylene; CY5.5 cyanine dye; microplastic; plastic; animal experiment; animal model; animal tissue; Article; body weight; computer assisted tomography; controlled study; female; gastrointestinal tract; in vitro study; lethal dose; male; molecular stability; mouse; nonhuman; particle size; toxicity; toxicology; animal; human; Institute for Cancer Research mouse; mammal; tissue distribution; water pollutant | English | 2023 | 2023-05-09 | 10.3390/ijms24108463 | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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