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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | ○ | Article | Dynamics of Humic Acid, Silicon, and Biochar under Heavy Metal, Drought, and Salinity with Special Reference to Phytohormones, Antioxidants, and Melatonin Synthesis in Rice | This study aimed to develop a biostimulant formulation using humic acid (HA), silicon, and biochar alone or in combination to alleviate the lethality induced by combined heavy metals (HM-C; As, Cd, and Pb), drought stress (DS; 30-40% soil moisture), and salt stress (SS; 150 mM NaCl) in rice. The results showed that HA, Si, and biochar application alone or in combination improved plant growth under normal, DS, and SS conditions significantly. However, HA increased the lethality of rice by increasing the As, Cd, and Pb uptake significantly, thereby elevating lipid peroxidation. Co-application reduced abscisic acid, elevated salicylic acid, and optimized the Ca2+ and Si uptake. This subsequently elevated the K+/Na+ influx and efflux by regulating the metal ion regulators (Si: Lsi1 and Lsi2; K+/Na+: OsNHX1) and increased the expressions of the stress-response genes OsMTP1 and OsNramp in the rice shoots. Melatonin synthesis was significantly elevated by HM-C (130%), which was reduced by 50% with the HA + Si + biochar treatment. However, in the SS- and DS-induced crops, the melatonin content showed only minor differences. These findings suggest that the biostimulant formulation could be used to mitigate SS and DS, and precautions should be taken when using HA for heavy metal detoxification. | Adhikari, Arjun; Aneefi, Appiah Gregory; Sisuvanh, Hairkham; Singkham, Santivong; Pius, Masele Valentine; Akter, Farida; Kwon, Eun-Hae; Kang, Sang-Mo; Woo, Youn-Ji; Yun, Byung-Wook; Lee, In-Jung; Posmyk, Malgorzata M. | Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea | Lee, In-Jung/GLS-0432-2022; Adhikari, Arjun/JCO-3306-2023; Kang, Sang-Mo/MBG-7823-2025 | 57195601415; 58777395600; 58776802100; 58776802200; 58776420800; 58777395700; 57224398710; 56189696900; 58776614000; 8245123600; 16425830900 | arjun@knu.ac.kr;gregory22@knu.ac.kr;hairkham@gmail.com;singkham2020@gmail.com;valentinemasele2017@gmail.com;akterfarida1985@knu.ac.kr;eunhae.kwon1@gmail.com;kmoya@hanmail.net;yjwoo2363@gmail.com;bwyun@knu.ac.kr;ijlee@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 24 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 1.2 | 2025-06-25 | 9 | 9 | abiotic stress; environment; biostimulants; detoxification; melatonin; sustainable agriculture; toxicity | TOLERANCE PROTEIN; STRESS TOLERANCE; ABSCISIC-ACID; SALT STRESS; PLANTS; TRANSPORTER; EXPRESSION; DEFENSE; ACCUMULATION; INVOLVEMENT | abiotic stress; biostimulants; detoxification; environment; melatonin; sustainable agriculture; toxicity | Antioxidants; Cadmium; Droughts; Humic Substances; Lead; Melatonin; Metals, Heavy; Oryza; Plant Growth Regulators; Salinity; Silicon; abscisic acid; arsenic; cadmium; calcium ion; charcoal; humic acid; lead; malonaldehyde; melatonin; natural resistance associated macrophage protein; proline; salicylic acid; silicon; superoxide dismutase; abscisic acid; antioxidant; calcium ion; heavy metal; lead; melatonin; metal ion; phytohormone; salicylic acid; abiotic stress; arsenic poisoning; Article; cadmium poisoning; chlorophyll content; controlled study; drought stress; lead poisoning; lethality; limit of quantitation; lipid peroxidation; nonhuman; relative humidity; rice; root weight; salt stress; shoot length; soil acidity; soil health; soil treatment; synthesis; abiotic stress; article; detoxification; drought; drought stress; pharmacology; plant growth; salinity; salt stress; soil moisture | English | 2023 | 2023-12 | 10.3390/ijms242417369 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Enantioselective catalysts based on metal-organic framework-supported nucleotides | Adenosine triphosphate (ATP) and other nucleotides can be irreversibly bound to the metal-organic framework (MOF) MIL-101(Cr). Analysis of X-ray diffraction data suggests that the location of the adsorbed ATP molecule is in proximity of the Cr3 clusters. Solid-state NMR and DFT calculations indicate that ATP is bound to MIL-101(Cr) through linkages of the terminal phosphate group with Cr(III) of the framework. In the presence of Cu(II) ions, the MOF-supported nucleotides can function as stable and reusable enantioselective heterogeneous catalysts for reactions like Diels-Alder and Michael addition. Compared to the corresponding homogeneous nucleotide-based artificial metalloenzymes (ArMs), the MOF-supported nucleotide-based ArMs exhibit significantly enhanced activity and selectivity in certain cases, demonstrating their potential as a new class of enantioselective heterogeneous catalysts. | Wang, Danyu; Li, Zhe; Luo, Tian-Yi; Schmithorst, Michael B.; Park, Sunghwan; Xu, Wenqian; Miao, Yurun; Gawande, Kaivalya; Tang, Chaoyun; Bukowski, Brandon C.; Chmelka, Bradley F.; Fairbrother, Howard; Kokkoli, Efrosini; Tsapatsis, Michael | Johns Hopkins Univ, Inst NanoBioTechnol, 3400 N Charles St, Baltimore, MD 21218 USA; Johns Hopkins Univ, Dept Chem & Biomol Engn, 3400 N Charles St, Baltimore, MD 21218 USA; Univ Calif Santa Barbara, Dept Chem Engn, Santa Barbara, CA 93106 USA; Kyungpook Natl Univ, Sch Energy Mat & Chem Engn, 2559 Gyeongsang Daero, Sangju Si 37224, Gyeongsangbug D, South Korea; Argonne Natl Lab, X Ray Sci Div, Adv Photon Source, Lemont, IL 60439 USA; Univ Massachusetts Amherst, Dept Chem Engn, Amherst, MA 01003 USA; Johns Hopkins Univ, Dept Chem, 3400 N Charles St, Baltimore, MD 21218 USA; Johns Hopkins Univ, Appl Phys Lab, 11100 Johns Hopkins Rd, Laurel, MD 20723 USA | ; Tsapatsis, Michael/AAA-2527-2019; Kokkoli, Efrosini/J-1656-2015; Tang, Chaoyun/U-1817-2019; Luo, Tianyi/ABE-8919-2020 | 57486531500; 57200539350; 56844539700; 57223848252; 56402062100; 24825901500; 56516069000; 58131296200; 55546205300; 56479156400; 7005062811; 7004543225; 6603552071; 7005981012 | kokkoli@jhu.edu;tsapatsis@jhu.edu; | MICROPOROUS AND MESOPOROUS MATERIALS | MICROPOR MESOPOR MAT | 1387-1811 | 1873-3093 | 360 | SCIE | CHEMISTRY, APPLIED;CHEMISTRY, PHYSICAL;MATERIALS SCIENCE, MULTIDISCIPLINARY;NANOSCIENCE & NANOTECHNOLOGY | 2023 | 4.8 | 20.9 | 0.11 | 2025-06-25 | 1 | 1 | Metal-organic framework; Artificial metalloenzyme; Asymmetric catalysis; Heterogenous catalysis | DIELS-ALDER REACTION; AUXILIARY BASIS-SETS; ARTIFICIAL METALLOENZYMES; HYBRID CATALYSTS; DNA; WATER; SEPARATION; DESIGN; SITES | Artificial metalloenzyme; Asymmetric catalysis; Heterogenous catalysis; Metal-organic framework | Addition reactions; Catalysis; Catalyst selectivity; Chromium compounds; Copper compounds; Enantioselectivity; Enzymes; Nuclear magnetic resonance spectroscopy; Organometallics; Adenosine triphosphate; Artificial metalloenzymes; Asymmetric catalysis; Enantioselective; Enantioselective catalysts; Heterogeneous catalyst; Heterogenous catalysis; Metalorganic frameworks (MOFs); Solid state DFT; X-ray diffraction data; Nucleotides | English | 2023 | 2023-10 | 10.1016/j.micromeso.2023.112703 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Engineering an Optimal Y280-Lineage H9N2 Vaccine Strain by Tuning PB2 Activity | H9N2 avian influenza A viruses (AIVs) cause economic losses in the poultry industry and provide internal genomic segments for the evolution of H5N1 and H7N9 AIVs into more detrimental strains for poultry and humans. In addition to the endemic Y439/Korea-lineage H9N2 viruses, the Y280-lineage spread to Korea since 2020. Conventional recombinant H9N2 vaccine strains, which bear mammalian pathogenic internal genomes of the PR8 strain, are pathogenic in BALB/c mice. To reduce the mammalian pathogenicity of the vaccine strains, the PR8 PB2 was replaced with the non-pathogenic and highly productive PB2 of the H9N2 vaccine strain 01310CE20. However, the 01310CE20 PB2 did not coordinate well with the hemagglutinin (HA) and neuraminidase (NA) of the Korean Y280-lineage strain, resulting in a 10-fold lower virus titer compared to the PR8 PB2. To increase the virus titer, the 01310CE20 PB2 was mutated (I66M-I109V-I133V) to enhance the polymerase trimer integrity with PB1 and PA, which restored the decreased virus titer without causing mouse pathogenicity. The reverse mutation (L226Q) of HA, which was believed to decrease mammalian pathogenicity by reducing mammalian receptor affinity, was verified to increase mouse pathogenicity and change antigenicity. The monovalent Y280-lineage oil emulsion vaccine produced high antibody titers for homologous antigens but undetectable titers for heterologous (Y439/Korea-lineage) antigens. However, this defect was corrected by the bivalent vaccine. Therefore, the balance of polymerase and HA/NA activities can be achieved by fine-tuning PB2 activity, and a bivalent vaccine may be more effective in controlling concurrent H9N2 viruses with different antigenicities. | An, Se-Hee; Hong, Seung-Min; Song, Jin-Ha; Son, Seung-Eun; Lee, Chung-Young; Choi, Kang-Seuk; Kwon, Hyuk-Joon | Seoul Natl Univ, Coll Vet Med, Lab Avian Dis, Seoul 08826, South Korea; Seoul Natl Univ, BK21 PLUS Vet Sci, Seoul 08826, South Korea; Seoul Natl Univ, Res Inst Vet Sci, Coll Vet Med, Seoul 08826, South Korea; Kyungpook Natl Univ, Sch Med, Dept Microbiol, Daegu 41566, South Korea; Seoul Natl Univ, Coll Vet Med, Dept Farm Anim Med, Lab Poultry Med, Seoul 88026, South Korea; Seoul Natl Univ, Farm Anim Clin Training & Res Ctr FACTRC, GBST, Pyeongchang 25354, South Korea; GeNiner Ltd, Seoul 08826, South Korea | 57195539685; 55512474800; 57221101773; 57221094613; 57195422071; 35798047900; 57196596247 | kchoi0608@snu.ac.kr;kwonhj01@snu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 10 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0.54 | 2025-06-25 | 5 | 5 | avian influenza A virus; recombinant vaccine strain; mammalian pathogenicity; bivalent oil emulsion vaccine | AVIAN INFLUENZA-VIRUSES; AMINO-ACID SUBSTITUTION; RECEPTOR-BINDING; A VIRUSES; INTERNAL GENES; PATHOGENICITY; HEMAGGLUTININ; VIRULENCE; GENERATION; INHIBITORS | avian influenza A virus; bivalent oil emulsion vaccine; H9N2; mammalian pathogenicity; recombinant vaccine strain | Animals; Chickens; Humans; Influenza A Virus, H5N1 Subtype; Influenza A Virus, H7N9 Subtype; Influenza A Virus, H9N2 Subtype; Influenza in Birds; Mammals; Mice; Vaccines, Combined; Vaccines, Synthetic; avian influenza vaccine; epitope; inactivated virus vaccine; Influenza virus hemagglutinin; recombinant vaccine; sialidase; recombinant vaccine; vaccine; animal cell; animal experiment; animal model; antibody blood level; antibody titer; antigenicity; Article; Bagg albino mouse; body weight loss; controlled study; drug efficacy; enzyme activity; enzyme inhibition; female; Gallus gallus; genetic engineering; genotype; hemagglutination inhibition test; human; human cell; humoral immunity; Influenza A virus (H5N1); Influenza A virus (H7N9); Influenza A virus (H9N2); mouse; nonhuman; poultry; revertant; survival rate; vaccine immunogenicity; viral genomics; virus isolation; virus load; virus mutation; virus recombinant; virus strain; virus titration; virus virulence; animal; avian influenza; genetics; Influenza A virus (H5N1); Influenza A virus (H7N9); mammal | English | 2023 | 2023-05-16 | 10.3390/ijms24108840 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Exosome from IFN-γ-Primed Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Improved Skin Inflammation and Barrier Function | The pathogenesis of atopic dermatitis (AD) is multifactorial, including immune dysregulation and epidermal barrier defects, and a novel therapeutic modality that can simultaneously target multiple pathways is needed. We investigated the therapeutic effects of exosomes (IFN-& gamma;-iExo) secreted from IFN-& gamma;-primed induced pluripotent stem cell-derived mesenchymal stem cells (iMSC) in mice with Aspergillus fumigatus-induced AD. IFN-& gamma;-iExo was epicutaneously administered to mice with AD-like skin lesions. The effects of IFN-& gamma;-iExo treatment were investigated through clinical scores, transepidermal water loss (TEWL) measurements, and histopathology. To elucidate the therapeutic mechanism, we used an in vitro model of human keratinocyte HaCaT cells stimulated with IL-4 and IL-13 and performed extensive bioinformatics analysis of skin mRNA from mice. The expression of indoleamine 2,3-dioxygenase was higher in IFN-& gamma; primed iMSCs than in iMSCs. In human keratinocyte HaCaT cells, treatment with IFN-& gamma;-iExo led to decreases in the mRNA expression of thymic stromal lymphopoietin, IL-25, and IL-33 and increases in keratin 1, keratin 10, desmoglein 1, and ceramide synthase 3. IFN-& gamma;-iExo treatment significantly improved clinical and histological outcomes in AD mice, including clinical scores, TEWL, inflammatory cell infiltration, and epidermal thickness. Bioinformatics analysis of skin mRNA from AD mice showed that IFN-& gamma;-iExo treatment is predominantly involved in skin barrier function and T cell immune response. Treatment with IFN-& gamma;-iExo improved the clinical and histological outcomes of AD mice, which were likely mediated by restoring proper skin barrier function and suppressing T cell-mediated immune response. | Yoon, Jin; Lee, Seul Ki; Park, Arum; Lee, Jiho; Jung, Inuk; Song, Kun Baek; Choi, Eom Ji; Kim, Soo; Yu, Jinho | Asan Med Ctr, Asan Inst Life Sci, Seoul 05505, South Korea; Brexogen Inc, Brexogen Res Ctr, Seoul 05855, South Korea; Kyungpook Natl Univ, Sch Comp Sci & Engn, Daegu 41566, South Korea; Univ Ulsan, Coll Med, Asan Med Ctr, Dept Pediat, Seoul 05505, South Korea | Lee, Seung-Hyun/A-6106-2013 | 55383547100; 55879487900; 57206857390; 57285314600; 56067575500; 57223855265; 57313056700; 57196486505; 8586114400 | yoonjin8787@gmail.com;seulki.lee@brexogen.com;alal6824@gmail.com;gis6093@daum.net;inukjung@knu.ac.kr;dizz2@hanmail.net;calleomza@naver.com;sue.kim@brexogen.com;jyu3922@gmail.com; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 14 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 2.14 | 2025-06-25 | 18 | 17 | atopic dermatitis; exosome; interferon-gamma; mesenchymal stem cell | EXTRACELLULAR VESICLES; ATOPIC-DERMATITIS; IFN-GAMMA; MECHANISMS; DISEASE | atopic dermatitis; exosome; interferon-gamma; mesenchymal stem cell | Animals; Cytokines; Dermatitis, Atopic; Exosomes; Humans; Induced Pluripotent Stem Cells; Inflammation; Interferon-gamma; Mesenchymal Stem Cells; Mice; RNA, Messenger; Skin; Water; 5' nucleotidase; cytokeratin 1; cytokeratin 10; desmoglein 1; dexamethasone; endoglin; immunoglobulin E; indoleamine 2,3 dioxygenase; interleukin 13; interleukin 25; interleukin 33; interleukin 4; messenger RNA; recombinant gamma interferon; sphingosine acyltransferase; thymic stromal lymphopoietin; water; cytokine; gamma interferon; messenger RNA; water; animal cell; animal experiment; animal model; animal tissue; Article; aspergillosis; Aspergillus fumigatus; atopic dermatitis; bioinformatics; cell infiltration; cellular immunity; confocal laser scanning microscopy; controlled study; cutaneous drug administration; dermatomycosis; differential gene expression; drug megadose; eosinophil count; epidermal thickness; exosome; female; fluorescence activated cell sorting; HaCat cell line; histopathology; human; human cell; immunoblotting; immunocytochemistry; immunofluorescence assay; immunoglobulin blood level; immunotherapy; in vitro study; induced pluripotent stem cell; inflammatory cell; KEGG; keratinization; low drug dose; lymphocyte count; lymphocyte differentiation; mesenchymal stem cell transplantation; mouse; mRNA expression level; neutrophil count; nonhuman; pathway enrichment analysis; pluripotent stem cell; protein expression level; real time reverse transcription polymerase chain reaction; skin defect; skin permeability; skin water loss; supernatant; Th1 cell; Th17 cell; Th2 cell; tissue regeneration; transmission electron microscopy; ultracentrifugation; animal; atopic dermatitis; inflammation; mesenchymal stem cell; metabolism; skin | English | 2023 | 2023-07 | 10.3390/ijms241411635 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Fabrication, Structural Characteristics, and Properties of Sericin-Coated Wool Nonwoven Fabrics | Recently, nonwoven fabrics from natural silk have attracted considerable attention for biomedical and cosmetic applications because of their good mechanical properties and cytocompatibility. Although these fabrics can be easily fabricated using the binding character of sericin, the high cost of silk material may restrict its industrial use in certain areas. In this study, sericin was added as a binder to a cheaper material (wool) to prepare wool-based nonwoven fabrics and investigate the effect of the amount of sericin added on the structural characteristics and properties of the wool nonwoven fabric. It was found using SEM that sericin coated the surface of wool fibers and filled the space between them. With an increase in sericin addition, the porosity, moisture regain, and the contact angle of the sericin-coated wool nonwoven fabric decreased. The maximum stress and initial Young's modulus of the nonwoven fabric increased with the increase in sericin amount up to 32.5%, and decreased with a further increase in the amount of sericin. Elongation at the end steadily decreased with the increase in sericin addition. All of the nonwoven fabrics showed good cytocompatibility, which increased with the amount of sericin added. These results indicate that sericin-coated wool-based nonwoven fabrics may be successfully prepared by adding sericin to wool fibers, and that the properties of these fabrics may be diversely controlled by altering the amount of sericin added, making them promising candidates for biomedical and cosmetic applications. | Lee, Hye Gyeoung; Jang, Mi Jin; Um, In Chul | Kyungpook Natl Univ, Dept Biofibers & Biomat Sci, Daegu 41566, South Korea; Daegu Gyeongbuk Med Innovat Fdn, Preclin Res Ctr, Daegu 41061, South Korea | 58550127900; 57222997201; 7006725707 | icum@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 19 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0.4 | 2025-06-25 | 2 | 3 | wool; sericin; nonwoven fabric; structural characteristics; mechanical properties; cell viability | GELATION BEHAVIOR; SILK SERICIN; KERATIN; FIBERS; PROTEIN; FTIR | cell viability; mechanical properties; nonwoven fabric; sericin; structural characteristics; wool | Animals; Sericins; Silk; Textiles; Wool; Wool Fiber; keratin; sericin; silk; animal cell; aqueous solution; Article; Bombyx mori; cell viability; comparative study; compression; conformation; contact angle; controlled study; crystal structure; dry weight; field emission scanning electron microscopy; fluorescence; morphological trait; nanofabrication; nonhuman; porosity; scanning electron microscopy; surface property; tensile strength; water absorption; X ray diffraction; animal; chemistry | English | 2023 | 2023-10 | 10.3390/ijms241914750 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Fabrication, Structure, and Properties of Nonwoven Silk Fabrics Prepared with Different Cocoon Layers | In this study, five different nonwoven silk fabrics were fabricated with silk fibers from different cocoon layers, and the effect of the cocoon layer on the structural characteristics and properties of the nonwoven silk fabric was examined. The diameter of the silk fiber and thickness of the nonwoven silk fabric decreased from the outer to the inner cocoon layer. More amino acids with higher hydrophilicity (serine, aspartic acid, and glutamic acid) and lower hydrophilicity (glycine and alanine) were observed in the outer layers. From the outer to the inner layer, the overall crystallinity and contact angle of the nonwoven silk fabric increased, whereas its yellowness index, moisture retention, and mechanical properties decreased. Regardless of the cocoon layer at which the fiber was sourced, the thermal stability of fibroin and sericin and good cell viability remained unchanged. The results of this study indicate that the properties of nonwoven silk fabric can be controlled by choosing silk fibers from the appropriate cocoon layers. Moreover, the findings in this study will increase the applicability of nonwoven silk fabric in the biomedical and cosmetic fields, which require specific properties for industrialization. | Choi, Yun Yeong; Jang, Mi Jin; Park, Byung-Dae; Um, In Chul | Kyungpook Natl Univ, Dept Biofibers & Biomat Sci, Daegu 41566, South Korea; Daegu Gyeongbuk Med Innovat Fdn, Preclin Res Ctr, Daegu 41061, South Korea; Kyungpook Natl Univ, Dept Wood & Paper Sci, Daegu 41566, South Korea | ; Park, Byung-Dae/ABB-1934-2020 | 58509793700; 57222997201; 7402834820; 7006725707 | icum@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 14 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0.8 | 2025-06-25 | 7 | 6 | silk web; nonwoven silk fabric; silkworm cocoon layer; structural characteristics; mechanical properties; cell viability | MOLECULAR-WEIGHT; MECHANICAL-PROPERTIES; PHYSICAL-PROPERTIES; GELATION BEHAVIOR; FIBROIN MEMBRANE; SERICIN; CRYSTALLINITY; REGENERATION; CONFORMATION; VARIETY | cell viability; mechanical properties; nonwoven silk fabric; silk web; silkworm cocoon layer; structural characteristics | Animals; Bombyx; Cell Survival; Fibroins; Sericins; Silk; Textiles; alanine; amino acid; aspartic acid; fibroin; glutamic acid; glycine; sericin; serine; silk; silk fibroin; fibroin; sericin; silk; amino acid composition; Article; Bombyx mori; breaking strength; cell viability; cocoon; color; conformation; contact angle; controlled study; crystal structure; cytotoxicity test; decomposition; differential scanning calorimetry; field emission scanning electron microscopy; Fourier transform infrared spectroscopy; hydrophilicity; industrialization; moisture; nonhuman; structure analysis; synthesis; tensile strength; thermostability; thickness; water absorption; X ray diffraction; animal; Bombyx; cell survival; chemistry | English | 2023 | 2023-07 | 10.3390/ijms241411485 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Genome-Engineered mpkCCDc14 Cells as a New Resource for Studying AQP2 | mpkCCDc14 cells, a polarized epithelial cell line derived from mouse kidney cortical collecting ducts, are known to express the vasopressin V2 receptor (V2R) and aquaporin-2 (AQP2) that are responsive to vasopressin. However, a low abundance of the endogenous AQP2 protein in the absence of vasopressin and heterogeneity of AQP2 protein abundance among the cultured cells may limit the further application of the cell line in AQP2 studies. To overcome the limitation, we aimed to establish mpkCCDc14 cells constitutively expressing V2R and AQP2 via CRISPR/Cas9-mediated genome engineering technology (i.e., V2R-AQP2 cells). 3 '- and 5 '-Junction PCR revealed that the V2R-AQP2 expression cassette with a long insert size (similar to 2.2 kb) was correctly integrated. Immunoblotting revealed the expression of products of integrated Aqp2 genes. Cell proliferation rate and dDAVP-induced cAMP production were not affected by the knock-in of Avpr2 and Aqp2 genes. The AQP2 protein abundance was significantly higher in V2R-AQP2 cells compared with control mpkCCDc14 cells in the absence of dDAVP and the integrated AQP2 was detected. Immunocytochemistry demonstrated that V2R-AQP2 cells exhibited more homogenous and prominent AQP2 labeling intensity in the absence of dDAVP stimulation. Moreover, prominent AQP2 immunolabeling (both AQP2 and pS256-AQP2) in the apical domain of the genome-edited cells was observed in response to dDAVP stimulation, similar to that in the unedited control mpkCCDc14 cells. Taken together, mpkCCDc14 cells constitutively expressing V2R and AQP2 via genome engineering could be exploited for AQP2 studies. | Jang, Hyo-Ju; Park, Hye-Jeong; Choi, Hong Seok; Jung, Hyun Jun; Kwon, Tae-Hwan | Kyungpook Natl Univ, Sch Med, Dept Biochem & Cell Biol, Taegu 41944, South Korea; Kyungpook Natl Univ, Sch Med, Dept Biomed Sci, BK21 FOUR KNU Convergence Educ Program, Taegu 41944, South Korea; Johns Hopkins Univ, Dept Med, Div Nephrol, Sch Med, Baltimore, MD 21205 USA | ; Park, Joonhong/AAZ-9885-2020; Jung, Hyun Jun/LKM-4480-2024; Kwon, Tae-Hwan/ABA-1981-2020 | 57214330719; 57213039714; 58072531000; 36985354100; 7202206089 | hjung24@jhmi.edu;thkwon@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 2 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0.54 | 2025-06-25 | 4 | 4 | aquaporin-2; CRISPR/Cas9; genome engineering; vasopressin receptor | KIDNEY COLLECTING DUCT; MEMBRANE INSERTION; WATER CHANNELS; VASOPRESSIN; EXPRESSION; AQUAPORIN-2; PATHOPHYSIOLOGY; STIMULATION; PHYSIOLOGY; ABUNDANCE | aquaporin-2; CRISPR/Cas9; genome engineering; vasopressin receptor | Animals; Aquaporin 2; Cell Membrane; Deamino Arginine Vasopressin; Kidney Tubules, Collecting; Mice; Vasopressins; aquaporin 2; cyclic AMP; vasopressin V2 receptor; Aqp2 protein, mouse; aquaporin 2; argipressin[1 deamino]; vasopressin derivative; 3' untranslated region; 5' untranslated region; animal cell; antibody labeling; Article; cell labeling; cell proliferation rate; cell stimulation; controlled study; CRISPR-CAS9 system; epithelial cell line; gene cassette; gene expression; gene insertion; gene knock-in; gene locus; genetic engineering; homology directed repair; immunoblotting; immunocytochemistry; mouse; mpkCCDc14 cell line; nonhuman; polymerase chain reaction; protein expression; animal; cell membrane; kidney collecting tubule; metabolism | English | 2023 | 2023-01 | 10.3390/ijms24021684 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Genome-Wide Association Studies of Seven Root Traits in Soybean (Glycine max L.) Landraces | Soybean [Glycine max (L.) Merr.], an important oilseed crop, is a low-cost source of protein and oil. In Southeast Asia and Africa, soybeans are widely cultivated for use as traditional food and feed and industrial purposes. Given the ongoing changes in global climate, developing crops that are resistant to climatic extremes and produce viable yields under predicted climatic conditions will be essential in the coming decades. To develop such crops, it will be necessary to gain a thorough understanding of the genetic basis of agronomic and plant root traits. As plant roots generally lie beneath the soil surface, detailed observations and phenotyping throughout plant development present several challenges, and thus the associated traits have tended to be ignored in genomics studies. In this study, we phenotyped 357 soybean landraces at the early vegetative (V2) growth stages and used a 180 K single-nucleotide polymorphism (SNP) soybean array in a genome-wide association study (GWAS) conducted to determine the phenotypic relationships among root traits, elucidate the genetic bases, and identify significant SNPs associated with root trait-controlling genomic regions/loci. A total of 112 significant SNP loci/regions were detected for seven root traits, and we identified 55 putative candidate genes considered to be the most promising. Our findings in this study indicate that a combined approach based on SNP array and GWAS analyses can be applied to unravel the genetic basis of complex root traits in soybean, and may provide an alternative high-resolution marker strategy to traditional bi-parental mapping. In addition, the identified SNPs, candidate genes, and diverse variations in the root traits of soybean landraces will serve as a valuable basis for further application in genetic studies and the breeding of climate-resilient soybeans characterized by improved root traits. | Kim, Seong-Hoon; Tayade, Rupesh; Kang, Byeong-Hee; Hahn, Bum-Soo; Ha, Bo-Keun; Kim, Yoon-Ha | RDA, Natl Inst Agr Sci, Natl Agrobiodivers Ctr, Jeonju, South Korea; Chonnam Natl Univ, Dept Appl Plant Sci, Gwangju 61186, South Korea; Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea | ; Kim, Seong-Hoon/AHE-2059-2022; Tayade, Rupesh/AAM-9652-2021 | 57208236056; 57191753234; 58046054200; 7201799276; 7006749575; 57224866763 | bkha@jnu.ac.kr;kyh1229@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 1 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 1.61 | 2025-06-25 | 12 | 12 | soybean; root trait; GWAS; SNP; candidate gene | GROWTH | candidate gene; GWAS; root trait; SNP; soybean | Chromosome Mapping; Genome, Plant; Genome-Wide Association Study; Linkage Disequilibrium; Phenotype; Plant Breeding; Polymorphism, Single Nucleotide; Quantitative Trait Loci; Soybeans; article; breeding; climate; crop; genetic association; genetic marker; genome-wide association study; genomics; growth curve; human cell; landrace; nonhuman; phenotype; plant development; plant root; single nucleotide polymorphism array; soil; soybean; chromosomal mapping; gene linkage disequilibrium; genetics; metabolism; plant breeding; plant genome; quantitative trait locus; single nucleotide polymorphism | English | 2023 | 2023-01 | 10.3390/ijms24010873 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Genome-Wide Identification and Comprehensive Analysis of the GASA Gene Family in Peanuts (Arachis hypogaea L.) under Abiotic Stress | Peanut (Arachis hypogaea L.) is a globally cultivated crop of significant economic and nutritional importance. The role of gibberellic-acid-stimulated Arabidopsis (GASA) family genes is well established in plant growth, development, and biotic and abiotic stress responses. However, there is a gap in understanding the function of GASA proteins in cultivated peanuts, particularly in response to abiotic stresses such as drought and salinity. Thus, we conducted comprehensive in silico analyses to identify and verify the existence of 40 GASA genes (termed AhGASA) in cultivated peanuts. Subsequently, we conducted biological experiments and performed expression analyses of selected AhGASA genes to elucidate their potential regulatory roles in response to drought and salinity. Phylogenetic analysis revealed that AhGASA genes could be categorized into four distinct subfamilies. Under normal growth conditions, selected AhGASA genes exhibited varying expressions in young peanut seedling leaves, stems, and roots tissues. Notably, our findings indicate that certain AhGASA genes were downregulated under drought stress but upregulated under salt stress. These results suggest that specific AhGASA genes are involved in the regulation of salt or drought stress. Further functional characterization of the upregulated genes under both drought and salt stress will be essential to confirm their regulatory roles in this context. Overall, our findings provide compelling evidence of the involvement of AhGASA genes in the mechanisms of stress tolerance in cultivated peanuts. This study enhances our understanding of the functions of AhGASA genes in response to abiotic stress and lays the groundwork for future investigations into the molecular characterization of AhGASA genes. | Syed Nabi, Rizwana Begum; Lee, Myoung Hee; Cho, Kwang-Soo; Tayade, Rupesh; Kim, Sungup; Kim, Jung-In; Kim, Min-Young; Lee, Eunsoo; Lee, Jungeun; Kim, Sang-Woo; Oh, Eunyoung | Natl Inst Crop Sci, Dept Southern Area Crop Sci, RDA, Miryang 50424, South Korea; Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea | Tayade, Rupesh/AAM-9652-2021; Lee, Seung Eun/ABG-1607-2021; Lee, Daesu/G-6407-2011 | 57200232212; 56142671500; 55722770800; 57191753234; 56016191300; 57204539161; 57026508700; 57908074100; 57206732355; 57908263700; 57204882790 | ruhii.syed@gmail.com;kscholove@korea.kr;rupesh.tayade@gmail.com;kji1204@korea.kr;lavondy10@korea.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 23 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0.27 | 2025-06-25 | 1 | 2 | GASA; peanut; gibberellin; drought; salt stress; phylogenetic analysis; expression analysis | DROUGHT STRESS; CROSSTALK; TOLERANCE; PROTEIN; WHEAT; SALT | drought; expression analysis; GASA; gibberellin; peanut; phylogenetic analysis; salt stress | Arabidopsis; Arabidopsis Proteins; Arachis; Gene Expression Regulation, Plant; Phylogeny; Plant Proteins; Stress, Physiological; gibberellic acid; Arabidopsis protein; plant protein; abiotic stress; Article; biomass; biotic stress; cellular distribution; chlorophyll content; comparative study; controlled study; drought; drought stress; gene expression; multigene family; peanut; phylogenetic tree; phylogeny; plant development; plant growth; plant height; plant leaf; plant root; real time polymerase chain reaction; root length; salinity; salt stress; shoot length; soybean; tomato; Arabidopsis; Arachis; gene expression regulation; genetics; metabolism; physiological stress | English | 2023 | 2023-12 | 10.3390/ijms242317117 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | In Silico Characterisation of the Aedes aegypti Gustatory Receptors | Aedes aegypti, also known as the dengue mosquito or the yellow fewer mosquito, is the vector of dengue, chikungunya, Zika, Mayaro and yellow fever viruses. The A. aegypti genome contains an array of gustatory receptor (GR) proteins that are related to the recognition of taste. In this study, we performed in silico molecular characterization of all 72 A. aegypti GRs reported in the latest version of A. aegypti genome AaegL5. Phylogenetic analysis classified the receptors into three major clads. Multiple GRs were found to encode multiple transcripts. Physicochemical attributes such as the aliphatic index, hydropathicity index and isoelectric point indicated that A. aegypti gustatory receptors are highly stable and are tailored to perform under a variety of cellular environments. Analysis for subcellular localization indicated that all the GRs are located either in the extracellular matrix or the plasma membrane. Results also indicated that the GRs are distributed mainly on chromosomes 2 and 3, which house 22 and 49 GRs, respectively, whereas chromosome 1 houses only one GR. NCBI-CDD analysis showed the presence of a highly conserved 7tm₇ chemosensory receptor protein superfamily that includes gustatory and odorant receptors from insect species Anopheles gambiae and Drosophila melanogaster. Further, three significantly enriched ungapped motifs in the protein sequence of all 72 A. aegypti gustatory receptors were found. High-quality 3D models for the tertiary structures were predicted with significantly higher confidence, along with ligand-binding residues. Prediction of S-nitrosylation sites indicated the presence of target cysteines in all the GRs with close proximity to the ligand-bindings sites within the 3D structure of the receptors. In addition, two highly conserved motifs inside the GR proteins were discovered that house a tyrosine (Y) and a cysteine (C) residue which may serve as targets for NO-mediated tyrosine nitration and S-nitrosylation, respectively. This study will help devise strategies for functional genomic studies of these important receptor molecules in A. aegypti and other mosquito species through in vitro and in vivo studies. | Bibi, Maria; Hussain, Adil; Ali, Farman; Ali, Asad; Said, Fazal; Tariq, Kaleem; Yun, Byung-Wook | Abdul Wali Khan Univ Mardan, Dept Entomol, Khyber Pakhtunkhwa 23200, Pakistan; Kyungpook Natl Univ, Coll Agr & Life Sci, Dept Appl Biosci, Daegu 41566, South Korea | Ali, Farman/GVT-3127-2022; Tariq, kaleem/J-7875-2015; BIBI, MARIA/NDS-5312-2025; Hussain, Dr. Adil/K-6016-2018; Hussain, Adil/K-6016-2018; TARIQ, KALEEM/J-7875-2015; Ali, Asad/GWQ-7078-2022 | 58449868300; 41961162600; 57220735812; 58269166700; 57194265962; 56364312600; 8245123600 | adilhussain@awkum.edu.pk;bwyun@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 15 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0 | 2025-06-25 | 1 | 0 | Aedes aegypti; gustatory receptors; characterization; nitric oxide; S-nitrosylation | NITRIC-OXIDE; S-NITROSYLATION; MOSQUITOS DIPTERA; CIBARIAL SENSILLA; PROTEIN; IMMUNITY | Aedes aegypti; characterization; gustatory receptors; nitric oxide; S-nitrosylation | Aedes; Animals; Dengue; Drosophila melanogaster; Drosophila Proteins; Ligands; Mosquito Vectors; Phylogeny; Receptors, Cell Surface; Taste; Zika Virus; Zika Virus Infection; cysteine; nitric oxide; tyrosine; cell surface receptor; Drosophila protein; ligand; Aedes aegypti; amino acid sequence; Anopheles gambiae; Article; cell membrane; cellular distribution; chromosome 1; chromosome 2; chromosome 3; computer model; Drosophila melanogaster; extracellular matrix; in vitro study; in vivo study; isoelectric point; ligand binding; nitration; nonhuman; olfactory receptor; physical chemistry; protein motif; protein processing; S-nitrosylation; taste bud; Aedes; animal; dengue; genetics; mosquito vector; phylogeny; taste; Zika fever; Zika virus | English | 2023 | 2023-08 | 10.3390/ijms241512263 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Insensitive Effects of Inflammatory Cytokines on the Reference Genes of Synovial Fluid Resident-Mesenchymal Stem Cells Derived from Rheumatoid Arthritis Patients | Mesenchymal stem cells derived from rheumatoid arthritis patients (RA-MSCs) provide an understanding of a variety of cellular and immunological responses within the inflammatory milieu. Sustained exposure of MSCs to inflammatory cytokines is likely to exert an influence on genetic variations, including reference genes (RGs). The sensitive effect of cytokines on the reference genes of RA-SF-MSCs may be a variation factor affecting patient-derived MSCs as well as the accuracy and reliability of data. Here, we comparatively evaluated the stability levels of nine RG candidates, namely GAPDH, ACTB, B2M, EEF1A1, TBP, RPLP0, PPIA, YWHAZ, and HPRT1, to find the most stable ones. Alteration of the RG expression was evaluated in MSCs derived from the SF of healthy donors (H-SF-MSCs) and in RA-SF-MSCs using the geNorm and NormFinder software programs. The results showed that TBP, PPIA, and YWHAZ were the most stable RGs for the normalization of H-SF-MSCs and RA-SF-MSCs using RT-qPCR, whereas ACTB, the most commonly used RG, was less stable and performed poorly. Additionally, the sensitivity of RG expression upon exposure to proinflammatory cytokines (TNF-alpha and IL-1 beta) was evaluated. RG stability was sensitive in the H-SF-MSCs exposed to TNF-alpha and IL-1 beta but insensitive in the RA-SF-MSCs. Furthermore, the normalization of IDO expression using ACTB falsely diminished the magnitude of biological significance, which was further confirmed with a functional analysis and an IDO activity assay. In conclusion, the results suggest that TBP, PPIA, and YWHAZ can be used in SF-MSCs, regardless of their exposure to inflammatory cytokines. | Bok, Eun-Yeong; Kim, Saet-Byul; Thakur, Gitika; Choe, Yong-Ho; Oh, Seong-Ju; Hwang, Sun-Chul; Ock, Sun-A.; Rho, Gyu-Jin; Lee, Sang-Il; Lee, Won-Jae; Lee, Sung-Lim | Gyeongsang Natl Univ, Coll Vet Med, Jinju 52828, South Korea; Gyeongsang Natl Univ, Sch Med & Hosp, Dept Orthopaed Surg, Jinju 52727, South Korea; Rural Dev Adm RDA, Natl Inst Anim Sci NIAS, Anim Biotechnol Div, 1500 Kongjwipatjwi ro, Isero Myeon 565851, South Korea; Gyeongsang Natl Univ, Res Inst Life Sci, Jinju 52828, South Korea; Gyeongsang Natl Univ, Sch Med & Hosp, Dept Internal Med, Jinju 52727, South Korea; Gyeongsang Natl Univ, Sch Med & Hosp, Inst Hlth Sci, Jinju 52727, South Korea; Kyungpook Natl Univ, Coll Vet Med, Daegu 41566, South Korea | Lee, SangHun/GPW-6306-2022; Kim, Saet-Byul/AAK-7920-2021; bok, eun yeong/MYS-4388-2025; lee, sang/Q-4650-2019 | 57221645701; 57221645272; 57216199962; 57202927556; 57220159013; 55466671500; 6603398912; 6603725057; 7601416380; 57205486455; 16678898100 | eybok@gnu.ac.kr;sbkim4@gnu.ac.kr;gitika18oct@gnu.ac.kr;yhchoego@gmail.com;osj414@gnu.ac.kr;hscspine@gnu.ac.kr;ocksa@korea.kr;jinrho@gnu.ac.kr;goldgu@gnu.ac.kr;iamcyshd@knu.ac.kr;sllee@gnu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 20 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0.13 | 2025-06-25 | 1 | 1 | patient-derived mesenchymal stem cell; reference gene; rheumatoid arthritis; inflammatory cytokines; quantitative polymerase chain reaction | SUITABLE REFERENCE GENE; BONE-MARROW; IMMUNOSUPPRESSIVE PROPERTIES; HOUSEKEEPING GENES; STROMAL CELLS; STABILITY; PATHOGENESIS; EXPRESSION; INCREASE; DISEASE | inflammatory cytokines; patient-derived mesenchymal stem cell; quantitative polymerase chain reaction; reference gene; rheumatoid arthritis | Arthritis, Rheumatoid; Cytokines; Gene Expression Profiling; Humans; Mesenchymal Stem Cells; Real-Time Polymerase Chain Reaction; Reference Standards; Reproducibility of Results; Synovial Fluid; Tumor Necrosis Factor-alpha; APC protein; beta 2 microglobulin; beta actin; CD34 antigen; complementary DNA; cyclic citrullinated peptide antibody; cyclophilin A; endoglin; glyceraldehyde 3 phosphate dehydrogenase; Hermes antigen; indoleamine 2,3 dioxygenase; interleukin 1beta; peptides and proteins; protein eukaryotic translation elongation factor 1 alpha 1; protein hypoxanthine phosphoribosyltransferase 1; protein ribosomal protein large p0; protein tyrosine 3 monooxygenase tryptophan 5 monooxygenase activation zeta; receptor type tyrosine protein phosphatase C; rheumatoid factor; TATA binding protein; Thy 1 membrane glycoprotein; tumor necrosis factor; unclassified drug; cytokine; tumor necrosis factor; adipocyte; adipogenesis; antigen expression; Article; cell culture; cell differentiation; cell isolation; chondrocyte; chondrogenesis; clinical article; controlled study; cycle threshold value; DAS28; disease duration; DNA synthesis; female; flow cytometry; gene; gene expression; genetic stability; human; human cell; immunocytochemistry; major histocompatibility complex; mesenchymal stem cell; osteoblast; osteocyte; real time polymerase chain reaction; rheumatoid arthritis; RNA extraction; synovial fluid; gene expression profiling; genetics; metabolism; procedures; reproducibility; standard; synovial fluid | English | 2023 | 2023-10 | 10.3390/ijms242015159 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Review | Insights into the Structure-Property-Activity Relationship of Zeolitic Imidazolate Frameworks for Acid-Base Catalysis | Zeolitic imidazolate frameworks (ZIFs) have been extensively examined for their potential in acid-base catalysis. Many studies have demonstrated that ZIFs possess unique structural and physicochemical properties that allow them to demonstrate high activity and yield products with high selectivity. Herein, we highlight the nature of ZIFs in terms of their chemical formulation and the textural, acid-base, and morphological properties that strongly affect their catalytic performance. Our primary focus is the application of spectroscopic methods as instruments for analyzing the nature of active sites because these methods can allow an understanding of unusual catalytic behavior from the perspective of the structure-property-activity relationship. We examine several reactions, such as condensation reactions (the Knoevenagel condensation and Friedlander reactions), the cycloaddition of CO2 to epoxides, the synthesis of propylene glycol methyl ether from propylene oxide and methanol, and the cascade redox condensation of 2-nitroanilines with benzylamines. These examples illustrate the broad range of potentially promising applications of Zn-ZIFs as heterogeneous catalysts. | Timofeeva, Maria N.; Panchenko, Valentina N.; Jhung, Sung Hwa | Boreskov Inst Catalysis SB RAS, Prospekt Akad Lavrentieva 5, Novosibirsk 630090, Russia; Kyungpook Natl Univ, Dept Chem, Daegu 41566, South Korea; Kyungpook Natl Univ, Green Nano Mat Res Ctr, Daegu 41566, South Korea | Timofeeva, Maria/E-9277-2012; Jhung, Sung/AAO-6683-2021; Panchenko, Valentina/D-1068-2014 | 7005428141; 7102133466; 6701659467 | timofeeva@catalysis.ru;sung@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 5 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0.85 | 2025-06-25 | 16 | 17 | zeolitic imidazolate frameworks; acid-base properties; structural impact; chemical composition impact; particle size effect; catalytic properties | METAL-ORGANIC FRAMEWORKS; CRYSTAL-SIZE; KNOEVENAGEL CONDENSATION; REDOX CONDENSATION; WATER-ADSORPTION; CO2 ADSORPTION; ZIF-8; PERFORMANCE; CYCLOADDITION; ZSM-5 | acid–base properties; catalytic properties; chemical composition impact; particle size effect; structural impact; zeolitic imidazolate frameworks | Catalysis; Imidazoles; Structure-Activity Relationship; Zeolites; 2 nitroaniline; aniline derivative; benzylamine derivative; carbon dioxide; epoxide; imidazole derivative; metal organic framework; methanol; propylene glycol; propylene glycol methyl ether; propylene oxide; unclassified drug; zeolite; zeolitic imidazolate framework; zinc; imidazole derivative; acid base catalysis; catalysis; catalyst; chemical composition; chemical reaction; chemical structure; cycloaddition; desorption; Friedlander reaction; hydrophilicity; hydrophobicity; Knoevenagel condensation; oxidation reduction reaction; particle size; physical chemistry; polymerization; Review; spectroscopy; structure activity relation; synthesis; temperature; catalysis; chemistry; structure activity relation | English | 2023 | 2023-03 | 10.3390/ijms24054370 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Molecular Structure of Phosphoserine Aminotransferase from Saccharomyces cerevisiae | Phosphoserine aminotransferase (PSAT) is a pyridoxal 5'-phosphate-dependent enzyme involved in the second step of the phosphorylated pathway of serine biosynthesis. PSAT catalyzes the transamination of 3-phosphohydroxypyruvate to 3-phosphoserine using L-glutamate as the amino donor. Although structural studies of PSAT have been performed from archaea and humans, no structural information is available from fungi. Therefore, to elucidate the structural features of fungal PSAT, we determined the crystal structure of Saccharomyces cerevisiae PSAT (ScPSAT) at a resolution of 2.8 A. The results demonstrated that the ScPSAT protein was dimeric in its crystal structure. Moreover, the gate-keeping loop of ScPSAT exhibited a conformation similar to that of other species. Several distinct structural features in the halide-binding and active sites of ScPSAT were compared with its homologs. Overall, this study contributes to our current understanding of PSAT by identifying the structural features of fungal PSAT for the first time. | Jang, Jiyeon; Chang, Jeong Ho | Kyungpook Natl Univ, Dept Biol Educ, 80 Daehak Ro, Daegu 41566, South Korea; Kyungpook Natl Univ, Dept Biomed Convergence Sci & Technol, 80 Daehak Ro, Daegu 41566, South Korea | 58161773300; 57203598905 | jhcbio@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 6 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0.13 | 2025-06-25 | 1 | 1 | phosphoserine aminotransferase; protein crystallography; Saccharomyces cerevisiae; phylogenetic relationship; crystal structure; pyridoxal 5'-phosphate; molecular protein structure; yeast; fungi | OXIDIZED GLUTATHIONE FERMENTATION; SPERMIDINE SYNTHASE; SERINE BIOSYNTHESIS; CRYSTAL-STRUCTURE; ESCHERICHIA-COLI; PHOSPHORYLATED PATHWAY; METABOLISM; ENZYMES; BINDING; DEHYDROGENASE | crystal structure; fungi; molecular protein structure; phosphoserine aminotransferase; phylogenetic relationship; protein crystallography; pyridoxal 5′-phosphate; Saccharomyces cerevisiae; yeast | Amino Acid Sequence; Crystallography, X-Ray; Humans; Molecular Structure; Pyridoxal Phosphate; Saccharomyces cerevisiae; Transaminases; aminotransferase; glutamic acid; monomer; phosphoserine; phosphoserine aminotransferase; recombinant protein; unclassified drug; aminotransferase; phosphoserine aminotransferase; pyridoxal 5 phosphate; amino terminal sequence; Article; conformational transition; controlled study; crystal structure; crystallography; DNA sequencing; enzyme active site; enzyme conformation; enzyme structure; nonhuman; phylogeny; Saccharomyces cerevisiae; transamination; amino acid sequence; chemical structure; human; metabolism; X ray crystallography | English | 2023 | 2023-03 | 10.3390/ijms24065139 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Review | Nitric Oxide Acts as a Key Signaling Molecule in Plant Development under Stressful Conditions | Nitric oxide (NO), a colorless gaseous molecule, is a lipophilic free radical that easily diffuses through the plasma membrane. These characteristics make NO an ideal autocrine (i.e., within a single cell) and paracrine (i.e., between adjacent cells) signalling molecule. As a chemical messenger, NO plays a crucial role in plant growth, development, and responses to biotic and abiotic stresses. Furthermore, NO interacts with reactive oxygen species, antioxidants, melatonin, and hydrogen sulfide. It regulates gene expression, modulates phytohormones, and contributes to plant growth and defense mechanisms. In plants, NO is mainly produced via redox pathways. However, nitric oxide synthase, a key enzyme in NO production, has been poorly understood recently in both model and crop plants. In this review, we discuss the pivotal role of NO in signalling and chemical interactions as well as its involvement in the mitigation of biotic and abiotic stress conditions. In the current review, we have discussed various aspects of NO including its biosynthesis, interaction with reactive oxygen species (ROS), melatonin (MEL), hydrogen sulfide, enzymes, phytohormones, and its role in normal and stressful conditions. | Khan, Murtaza; Ali, Sajid; Al Azzawi, Tiba Nazar Ibrahim; Yun, Byung-Wook | Yeungnam Univ, Dept Hort & Life Sci, Gyongsan 38541, South Korea; Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea | Ali, Sajid/GLS-7322-2022 | 57207990116; 57214290889; 57224980187; 8245123600 | sajidbioali@gmail.com;bwyun@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 5 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 3.81 | 2025-06-25 | 64 | 73 | nitric oxide; antioxidants; phytohormones; signaling; stress; crop plants | LATERAL ROOT-FORMATION; S-NITROSYLATED PROTEINS; ABSCISIC-ACID; ARABIDOPSIS-THALIANA; NITRATE REDUCTASE; HYDROGEN-PEROXIDE; STOMATAL CLOSURE; SEED DORMANCY; ANTIOXIDANT DEFENSE; NO GENERATION | antioxidants; crop plants; nitric oxide; phytohormones; signaling; stress | Hydrogen Sulfide; Melatonin; Nitric Oxide; Plant Development; Plant Growth Regulators; Plants; Reactive Oxygen Species; Stress, Physiological; abscisic acid; auxin; cytokinin; ethylene; gibberellin; hydrogen sulfide; melatonin; nitrate; nitric oxide; nitric oxide synthase; nitrite reductase; phytohormone; reactive oxygen metabolite; hydrogen sulfide; melatonin; nitric oxide; phytohormone; reactive oxygen metabolite; abiotic stress; autocrine signaling; biosynthesis; biotic stress; cell membrane; chemical interaction; gene expression regulation; lipophilicity; nonhuman; oxidation reduction reaction; paracrine signaling; plant development; plant growth; Review; signal transduction; metabolism; physiological stress; plant; plant development | English | 2023 | 2023-03 | 10.3390/ijms24054782 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Nonsteroidal Anti-Inflammatory Drug Conjugated with Gadolinium (III) Complex as an Anti-Inflammatory MRI Agent | Studies have been actively conducted to ensure that gadolinium-based contrast agents for magnetic resonance imaging (MRI) are accompanied by various biological functions. A new example is the anti-inflammatory theragnostic MRI agent to target inflammatory mediators for imaging diagnosis and to treat inflammatory diseases simultaneously. We designed, synthesized, and characterized a Gd complex of 1,4,7-tris(carboxymethylaza) cyclododecane-10-azaacetylamide (DO3A) conjugated with a nonsteroidal anti-inflammatory drug (NSAID) that exerts the innate therapeutic effect of NSAIDs and is also applicable in MRI diagnostics. Gd-DO3A-fen (0.1 mmol/kg) was intravenously injected into the turpentine oil-induced mouse model, with Gd-DO3A-BT as a control group. In the in vivo MRI experiment, the contrast-to-noise ratio (CNR) was higher and persisted longer than that with Gd-DO3A-BT; specifically, the CNR difference was almost five times at 2 h after injection. Gd-DO3A-fen had a binding affinity (K-a) of 6.68 x 10(6) M-1 for the COX-2 enzyme, which was 2.1-fold higher than that of fenbufen, the original NSAID. In vivo evaluation of anti-inflammatory activity was performed in two animal models. In the turpentine oil-induced model, the mRNA expression levels of inflammatory parameters such as COX-2, TNF-alpha, IL-1 beta and IL-6 were reduced, and in the carrageenan-induced edema model, swelling was suppressed by 72% and there was a 2.88-fold inhibition compared with the saline group. Correlation analysis between in vitro, in silico, and in vivo studies revealed that Gd-DO3A-fen acts as an anti-inflammatory theragnostic agent by directly binding to COX-2. | Sung, Bokyung; Kim, Hee-Kyung; Baek, Ah-Rum; Yang, Byeong-Woo; Kim, Yeoun-Hee; Choi, Garam; Park, Hyun-Jin; Kim, Minsup; Lee, Jongmin; Chang, Yongmin | Kyungpook Natl Univ, Dept Med & Biol Engn, Daegu 41944, South Korea; Daegu Gyeongbuk Med Innovat Fdn K MEDIhub, Preclin Res Ctr, Daegu 41061, South Korea; Kyungpook Natl Univ, Inst Biomed Engn Res, Daegu 41566, South Korea; Etnova Therapeut Corp, R&D Ctr, Suwon 13120, South Korea; Korea Univ, Dept Biotechnol & Bioinformat, Sejong Campus,2511 Sejong Ro, Sejong City 30019, South Korea; Kyungpook Natl Univ Hosp, Dept Radiol, Daegu 41944, South Korea; Kyungpook Natl Univ, Sch Med, Dept Mol Med, Daegu 41944, South Korea | ; Yang, Byeong Woo/HII-8624-2022; Lee, Jongmin/MTC-9433-2025; Park, Hyun-Jin/HPE-8152-2023 | 58929528400; 56014729400; 57194601711; 57195807615; 57576538700; 56013903700; 57203526784; 56122945800; 55689919700; 7501840633 | priere4@gmail.com;hkkim@kmedihub.re.kr;ychang@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 7 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0.54 | 2025-06-25 | 3 | 4 | magnetic resonance imaging; contrast agent; NSAIDs; COX-2; anti-inflammation | COX-2 INHIBITORS; CONTRAST AGENTS; INFLAMMATION; CYCLOOXYGENASE-2; NSAIDS; CANCER; INDOMETHACIN; DERIVATIVES; FENBUFEN; DESIGN | anti-inflammation; contrast agent; COX-2; magnetic resonance imaging; NSAIDs | Animals; Anti-Inflammatory Agents; Anti-Inflammatory Agents, Non-Steroidal; Contrast Media; Cyclooxygenase 2; Gadolinium; Magnetic Resonance Imaging; Mice; Organometallic Compounds; Turpentine; fenbufen; gadolinium; gd do3a fen; interleukin 1beta; interleukin 6; nonsteroid antiinflammatory agent; radiopharmaceutical agent; unclassified drug; antiinflammatory agent; contrast medium; cyclooxygenase 2; gadolinium; nonsteroid antiinflammatory agent; organometallic compound; turpentine; animal experiment; animal model; antiinflammatory activity; Article; association constant; binding affinity; C2C12 cell line; contrast to noise ratio; controlled study; correlation analysis; cytotoxicity; extracellular fluid; histogram; in vitro study; in vivo study; inductively coupled plasma atomic emission spectrometry; lipophilicity; male; mouse; mRNA expression assay; mRNA expression level; nonhuman; nuclear magnetic resonance imaging; protein expression; Western blotting; whole body MRI; animal; chemistry; genetics; nuclear magnetic resonance imaging; procedures | English | 2023 | 2023-04 | 10.3390/ijms24076870 | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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