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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | ○ | Article | Association of Uncontrolled Hypertension or Diabetes Mellitus With Major Adverse Cardiovascular Events and Mortality in South Korea: Population-Based Cohort Study | Background: Managing hypertension (HT) and diabetes mellitus (DM) is crucial to preventing cardiovascular diseases. Few studies have investigated the incidence and risk of cardiovascular diseases or mortality in uncontrolled HT or DM in the Asian population. Epidemiological studies of cardiovascular disease should be conducted with continuous consideration of the changing disease risk profiles, lifestyles, and socioeconomic status over time. Objective: We aimed to examine the association of uncontrolled HT or DM with the incidence of cardiovascular events or deaths from any cause.Methods: This population-based retrospective study was conducted using data from the Korean National Health Insurance Service-National Health Screening Cohort, including patients aged 40-79 years who participated in national screening from 2002 to 2003 and were followed up until 2015. The health screening period from 2002 to 2013 was stratified into 6 index periods in 2-year cycles, and the follow-up period from 2004 to 2015 was stratified accordingly into 6 subsequent 2-year periods. The incidence rates and hazard ratio (HR) for major adverse cardiovascular events (MACE) and death from any cause were estimated according to HT or DM control status. Extended Cox models with time-dependent variables updated every 2 years, including sociodemographic characteristics, blood pressure (BP), fasting blood glucose (FBG), medication prescription, and adherence, were used.Results: Among the total cohort of 440,249 patients, 155,765 (35.38%) were in the uncontrolled HT or DM group. More than 60% of the patients with HT or DM who were prescribed medications did not achieve the target BP or FBG. The incidence of MACE was 10.8-15.5 and 9.6-13.3 per 1000 person-years in the uncontrolled DM and uncontrolled HT groups, respectively, and increased with age. In the uncontrolled HT and DM group, the incidence of MACE was high (15.2-17.5 per 1000 person-years) at a relatively young age and showed no age-related trend. Adjusted HR for MACE were 1.28 (95% CI 1.23-1.32) for the uncontrolled DM group, 1.32 (95% CI 1.29-1.35) for the uncontrolled HT group, and 1.54 (95% CI 1.47-1.60) for the uncontrolled HT and DM group. Adjusted HR for death from any cause were 1.05 (95% CI 1.01-1.10) for the uncontrolled DM group, 1.13 (95% CI 1.10-1.16) for the uncontrolled HT group, and 1.17 (95% CI 1.12-1.23) for the uncontrolled HT and DM group. Conclusions: This up-to-date evidence of cardiovascular epidemiology in South Korea serves as the basis for planning public health policies to prevent cardiovascular diseases. The high uncontrolled rates of HT or DM, regardless of medication prescription, have led us to suggest the need for a novel system for effective BP or glycemic control, such as a community-wide management program using mobile health technology. | Oh, Sung-Hee; Kim, Dohyang; Hwang, Jinseub; Kang, Jae-Heon; Kwon, Yeongkeun; Kwon, Jin-Won | Kyungpook Natl Univ, Coll Pharm, BK21 FOUR Community Based Intelligent Novel Drug D, Daegu, South Korea; Kyungpook Natl Univ, Res Inst Pharmaceut Sci, Daegu, South Korea; Daegu Univ, Dept Stat, Gyeongbuk, South Korea; Sungkyunkwan Univ, Kangbuk Samsung Hosp, Coll Med, Dept Family Med, Seoul, South Korea; Korea Univ Anam Hosp, Ctr Obes & Metab Dis, Seoul, South Korea; Kyungpook Natl Univ, Coll Pharm, BK21 FOUR Community Based Intelligent Novel Drug D, 80 Daehak Ro,Buk Gu, Daegu 41566, South Korea; Kyungpook Natl Univ, Res Inst Pharmaceut Sci, 80 Daehak Ro,Buk Gu, Daegu 41566, South Korea | ; Kim, Il Young/LLK-4732-2024 | 57188550994; 57218821860; 57189231296; 57217443426; 55970203000; 16202951700 | jwkwon@knu.ac.kr; | JMIR PUBLIC HEALTH AND SURVEILLANCE | JMIR PUBLIC HLTH SUR | 2369-2960 | 9 | SCIE;SSCI | PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH | 2023 | 3.5 | 20.7 | 1.24 | 2025-06-25 | 7 | 7 | prevention and control; cardiovascular diseases; diabetes mellitus; hypertension; extended Cox regression; cohort study | BLOOD-PRESSURE; DISEASE; HEALTH; RISK; COMPLICATIONS; PREVALENCE; ADHERENCE | cardiovascular diseases; cohort study; diabetes mellitus; extended Cox regression; hypertension; prevention and control | Cardiovascular Diseases; Cohort Studies; Diabetes Mellitus; Humans; Hypertension; Retrospective Studies; cardiovascular disease; cohort analysis; diabetes mellitus; human; hypertension; retrospective study | English | 2023 | 2023 | 10.2196/42190 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Editorial Material | Controlled Single Freezing Cryotherapy for Mild Facial Actinic Keratosis | Ha, Nam Gyoung; Lee, Seon Hwa; Ha, Dae-Lyong; Park, Kyung Duck; Jang, Yong Hyun; Lee, Weon Ju; Lee, Seok-Jong; Kim, Jun Young | Kyungpook Natl Univ, Sch Med, Dept Dermatol, 130 Dongdeok Ro, Daegu 41944, South Korea | Lee, Joong/A-5417-2013 | 57772600900; 57224078421; 57201367090; 55767995700; 57016046400; 24474659000; 56013454400; 35310922800 | 198kjy@hanmail.net; | ACTA DERMATO-VENEREOLOGICA | ACTA DERM-VENEREOL | 0001-5555 | 1651-2057 | 103 | SCIE | DERMATOLOGY | 2023 | 3.5 | 20.7 | 0.63 | 2025-06-25 | 3 | 2 | AMINOLEVULINATE-PHOTODYNAMIC THERAPY; CRYOSURGERY; MULTICENTER | Cryotherapy; Fluorouracil; Freezing; Humans; Keratosis, Actinic; carbon; fluorouracil; actinic keratosis; aged; Article; clinical article; controlled study; cryotherapy; erythema; freeze thawing; human; hypopigmentation; recurrence risk; remission; retrospective study; skin; wrinkle; actinic keratosis; cryotherapy; freezing | English | 2023 | 2023-01 | 10.2340/actadv.v103.5313 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||||
| ○ | Meeting Abstract | HEAD AND NECK DERMATITIS IS EXACERBATED BY MALASSEZIA FURFUR COLONIZATION, SKIN BARRIER DISRUPTION, AND IMMUNE DYSREGULATION | Chu, Howard; Kim, Su Min; Zhang, Kelun; Wu, Zhexue; Lee, Hemin; Kim, Ji Hye; Kim, Hye Li; Kim, Yu Ri; Kim, Seo Hyeong; Kim, Wan Jin; Lee, Yang Won; Lee, Kwang Hoon; Liu, Kwang-Hyeon; Park, Chang Ook | Yonsei Univ, Cutaneous Biol Res Inst, Severance Hosp, Dept Dermatol,Coll Med, Seoul, South Korea; Kyungpook Natl Univ, Coll Pharm, BK21 FOUR Community Based Intelligent Novel Drug, Daegu, South Korea; Kyungpook Natl Univ, Res Inst Pharmaceut Sci, BK21 FOUR Community Based Intelligent Novel Drug, Daegu, South Korea; Konkuk Univ, Dept Dermatol, Sch Med, Seoul, South Korea | ZHANG, KELUN/NPJ-0253-2025; Lee, Jae-Hyun/ABE-3803-2020; Chu, Howard/IVH-3242-2023; Kim, Sumin/IZE-4757-2023; Kim, Sung-Soo/ABD-3771-2021 | ACTA DERMATO-VENEREOLOGICA | ACTA DERM-VENEREOL | 0001-5555 | 1651-2057 | 103 | SCIE | DERMATOLOGY | 2023 | 3.5 | 20.7 | 0 | English | 2023 | 2023 | 바로가기 | 바로가기 | ||||||||||||||||
| ○ | ○ | Article | Pellino-1 expression is associated with epidermal proliferation and enhanced Th17 cell infiltration in psoriatic lesions | Pellino-1 plays a crucial role in cellular proliferation and regulates inflammatory processes. This study investigated Pellino-1 expression patterns and their relationship with CD4(+) T-cell subsets in psoriasis patients. Group 1 comprised primarily biopsied psoriasis lesions from 378 patients, multiplex-immunostained for Pellino-1, CD4 and representative T helper (Th) cells (T-bet [Th1], GATA3 [Th2], and ROR?t [Th17] and regulatory T cell [FoxP3] markers). Ki-67 labeling was evaluated in the epidermis. Group 2 comprised 43 Pellino-1-positive cases immunostained for Pellino-1 in both lesion and non-lesion skin biopsy samples. Five normal skin biopsies served as controls. Among 378 psoriasis cases, 293 (77.5%) were positive for Pellino-1 in the epidermis. Pellino-1-positivity was higher in psoriasis lesions than in non-lesions and normal skin (52.55% vs. 40.43% vs. 3.48%, p < 0.001; H-score, 72.08 vs. 47.55 vs. 4.40, p < 0.001, respectively). Pellino-1-positive cases also had a significantly higher Ki-67 labeling index (p < 0.001). Epidermal Pellino1-positivity was significantly associated with higher ROR?t(+) (p = 0.001) and FoxP3(+) (p < 0.001) CD4(+) T cell ratios but not T-bet(+) and GATA3(+) CD4(+) T cell ratios. Among the CD4(+)Pellino-1(+) T-cell subsets, the CD4(+)Pellino-1(+)ROR?t(+) ratio was significantly associated with epidermal Pellinio-1 expression (p < 0.001). Pellino-1 expression is thus increased in psoriasis lesions and associated with increased epidermal proliferation and CD4(+) T-cell subset infiltration, especially Th17 cells. This suggests that Pellino-1 could be a therapeutic target that simultaneously regulates psoriasis epidermal proliferation and immune interactions. | Cho, Haeyon; Park, Nora Jee-Young; Ko, Jiwon; Lee, Chang-Woo; Lee, Jin-Kwan; Maeng, Young-In; Go, Heounjeong | Univ Ulsan, Asan Med Ctr, Dept Pathol, Coll Med, Seoul, South Korea; Kyungpook Natl Univ, Sch Med, Dept Pathol, Daegu, South Korea; Kyungpook Natl Univ, Dept Pathol, Chilgok Hosp, Daegu, South Korea; Kyungpook Natl Univ, Sch Med, Four Program BK21, Daegu, South Korea; Kyungpook Natl Univ, Clin Omics Inst, Daegu, South Korea; Univ Ulsan, Asan Inst Life Sci, Asan Med Ctr, Coll Med, Seoul, South Korea; Sungkyunkwan Univ, Samsung Med Ctr, Dept Mol Cell Biol, Sch Med, Suwon, South Korea; Curogen Co Ltd, Res Inst, Suwon, South Korea; Catholic Univ Daegu, Sch Med, Dept Pathol, Daegu, South Korea; Univ Ulsan, Asan Med Ctr, Dept Pathol, Coll Med, 88 Olymp ro,43 gil, Seoul 05505, South Korea | Lee, Chang-Woo/L-5093-2015 | 57164206800; 57226185359; 57222964743; 55700508300; 57002023400; 55834985000; 57204538826 | damul37@amc.seoul.kr; | EXPERIMENTAL DERMATOLOGY | EXP DERMATOL | 0906-6705 | 1600-0625 | 32 | 9 | SCIE | DERMATOLOGY | 2023 | 3.5 | 20.7 | 0 | 2025-06-25 | 0 | 0 | epidermal proliferation; Pellino-1; psoriasis; T helper cell; Th17 | E3 UBIQUITIN LIGASES; DENDRITIC CELLS; CD4 EXPRESSION; PATHOGENESIS; IL-17 | epidermal proliferation; Pellino-1; psoriasis; T helper cell; Th17 | Cell Proliferation; Epidermis; Forkhead Transcription Factors; Humans; Ki-67 Antigen; Nuclear Receptor Subfamily 1, Group F, Member 3; Psoriasis; Th17 Cells; CD4 antigen; pellino 1; retinoid related orphan receptor gamma; transcription factor FOXP3; transcription factor GATA 3; transcription factor T bet; ubiquitin protein ligase E3; unclassified drug; forkhead transcription factor; Ki 67 antigen; retinoid related orphan receptor gamma; adult; antibody labeling; Article; CD4+ T lymphocyte; cell infiltration; cell proliferation; controlled study; female; human; human cell; human tissue; immunohistochemistry; keratinocyte; labeling index; major clinical study; male; protein expression; psoriasis; regulatory T lymphocyte; retrospective study; skin biopsy; T lymphocyte subpopulation; Th1 cell; Th17 cell; Th2 cell; cell proliferation; epidermis; metabolism; psoriasis | English | 2023 | 2023-09 | 10.1111/exd.14852 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | Meeting Abstract | VALIDITY AND RELIABILITY OF ITCH INTENSITY SCALES FOR CHRONIC PRURITUS IN ATOPIC DERMATITIS AND CHRONIC URTICARIA | Bang, Jin Seon; Kim, Do Won; Jang, Yong Hyun | Kyungpook Natl Univ, Sch Med, Dept Dermatol, Daegu, South Korea; Kyungpook Natl Univ Hosp, Dept Dermatol, Daegu, South Korea | ACTA DERMATO-VENEREOLOGICA | ACTA DERM-VENEREOL | 0001-5555 | 1651-2057 | 103 | SCIE | DERMATOLOGY | 2023 | 3.5 | 20.7 | 0 | English | 2023 | 2023 | 바로가기 | 바로가기 | |||||||||||||||||
| ○ | Meeting Abstract | VARIATION IN HEMATOLOGICAL PARAMETERS ASSOCIATED WITH JANUS KINASES 1 AND 2 INHIBITION IN TWO PATIENTS | Bang, Jin Seon; Lee, Seung Soo; Ha, Dae-Lyong; Kim, Jun Young; Park, Kyung Duck; Lee, Weon Ju; Lee, Seok-Jong; Jang, Yong Hyun | Kyungpook Natl Univ Hosp, Dept Dermatol, Daegu, South Korea | Lee, Joong/A-5417-2013 | ACTA DERMATO-VENEREOLOGICA | ACTA DERM-VENEREOL | 0001-5555 | 1651-2057 | 103 | SCIE | DERMATOLOGY | 2023 | 3.5 | 20.7 | 0 | English | 2023 | 2023 | 바로가기 | 바로가기 | ||||||||||||||||
| ○ | ○ | Article | A Split-Inductor Flying Capacitor Converter for Medium-Voltage Application | This article presents a split-inductor flying capacitor (SIFC) converter for medium-voltage applications to improve reliability and eliminate the shoot-through issue. Reliability and lower total harmonic distortion (THD), mainly caused by the pulsewidth modulation (PWM) dead time, are the vital aspects while designing higher power density converters. The dead time in the PWM can be reduced/removed in the proposed converter. This will increase the voltage/current gain and enhance the quality of the voltage/current waveforms even at lower modulation index and light loads than the traditional flying capacitor (FC) and neutral point clamped (NPC) converters. Furthermore, an improved phase-shifted PWM (IPS-PWM) is used to maintain the flying capacitor's nominal reference voltage. A comparison is made between SIFC and five different existing multilevel topologies. The proposed converter has higher voltage/current gain, lower THD over conventional FC and NPC converters, and higher efficiency than the other three topologies. Finally, the SIFC converter's robustness with IPS-PWM is validated using experimental results in different operating conditions such as sudden load changes, modulation index, output frequency, and power factor (unity, lagging, and leading). | Faraji, Faramarz; Ghias, Amer M. Y. M.; Guo, Xiaoqiang; Chen, Zhaowen; Lu, Zhigang; Hua, Changchun | Kyungpook Natl Univ, Sch Energy Engn, Daegu 41566, South Korea; Nanyang Technol Univ, Sch Elect & Elect Engn, Singapore 639798, Singapore; Yanshan Univ, Dept Elect Engn, Qinhuangdao 066004, Peoples R China | ; Guo, xiaoqiang/AHB-6234-2022; Mohammad Ghias, Amer Mohammad Yusuf/E-5281-2017; Chen, Zhaowen/JXL-2828-2024; Faraji, Faramarz/J-4074-2019 | 57191226987; 46062041900; 14830056700; 57560938500; 55186088900; 34769628200 | Faraji.u@gmail.com;amer.ghias@ntu.edu.sg;yeduming@163.com;454435787@qq.com;zhglu@ysu.edu.cn;cch@ysu.edu.cn; | IEEE JOURNAL OF EMERGING AND SELECTED TOPICS IN POWER ELECTRONICS | IEEE J EM SEL TOP P | 2168-6777 | 2168-6785 | 11 | 1 | SCIE | ENGINEERING, ELECTRICAL & ELECTRONIC | 2023 | 4.6 | 20.8 | 0.69 | 2025-06-25 | 3 | 7 | Topology; Voltage; Pulse width modulation; Capacitors; Voltage control; Inductors; Inverters; High reliability; improved phase-shifted pulsewidth modulation (IPS-PWM); multilevel converter (MC); split-inductor flying capacitor (SIFC); total harmonic distortion (THD) | PHASE TRANSFORMERLESS INVERTER; LEAKAGE CURRENT ATTENUATION; DEAD-TIME COMPENSATION; DUAL-BUCK INVERTER; BALANCING METHOD; MULTILEVEL CONVERTER; SHIFTED PWM; TOPOLOGIES; TECHNOLOGIES; RELIABILITY | High reliability; improved phase-shifted pulsewidth modulation (IPS-PWM); multilevel converter (MC); split-inductor flying capacitor (SIFC); total harmonic distortion (THD) | Electric inductors; Electric inverters; Pulse width modulation; Reliability; Uninterruptible power systems; Voltage control; Flying capacitor; High reliability; Improved phase-shifted PWM; Inductor; Inverter; Multilevel converter; Phase-shifted PWM; Pulsewidth modulations (PWM); Split-inductor flying capacitor; Total harmonic distortions; Topology | English | 2023 | 2023-02 | 10.1109/jestpe.2022.3164130 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Universal and Non-Iterative Design Method for In-Phase Radiation of Microstrip Traveling-Wave Series-Fed Antenna Arrays | Microstrip traveling-wave series-fed antenna arrays (MTSAAs) have been popularly developed for radar systems in the millimeter-waveband. Because the antennas are connected and fed in series in MTSAA, in-phase radiation of the antenna elements with properly tapered amplitude for achieving a broadside beam with a low sidelobe level (SLL) is challenging. Although the reported series-fed patch and comb-line arrays accomplished the goal, design equations for the optimum distances between antenna elements in the series-fed array do not guarantee in-phase radiation due to the mutual coupling in array structures. Therefore, optimization of the distances in the array using full-wave simulations is inevitable. In this article, we propose the first universal and non-iterative design equation that compensates for the phase offset generated in patch and comb-line MTSAAs and successfully found optimum distances for broadside beams with low SLLs without optimization. Finally, we fabricated both MTSAAs with ten elements operating at 76.5 GHz and demonstrated almost 13 dB boresight gain and low SLLs near -18 dB without a beam tilt. | Lee, Sangmin; Choi, Sangjo | Kyungpook Natl Univ, Sch Elect & Elect Engn, Daegu 41566, South Korea | 57213176293; 36697690200 | sangmin2@knu.ac.kr;sangjoc@knu.ac.kr; | IEEE TRANSACTIONS ON ANTENNAS AND PROPAGATION | IEEE T ANTENN PROPAG | 0018-926X | 1558-2221 | 71 | 2 | SCIE | ENGINEERING, ELECTRICAL & ELECTRONIC;TELECOMMUNICATIONS | 2023 | 4.6 | 20.8 | 0.85 | 2025-06-25 | 4 | 7 | Antennas; Microstrip antenna arrays; Phased arrays; Mutual coupling; Mathematical models; Delays; Couplings; Comb-line array; millimeter-wave antenna array; series-fed patch array; traveling-wave series-fed antenna array | Comb-line array; millimeter-wave antenna array; series-fed patch array; traveling-wave series-fed antenna array | Antenna feeders; Antenna phased arrays; Microstrip antennas; Microwave antennas; Millimeter waves; Radar antennas; Slot antennas; Structural optimization; Traveling wave antennas; Comb-line array; Delay; Line arrays; Microstrip antenna array; Millimeter wave antenna arrays; Mutual coupling; Patch arrays; Phased-arrays; Series feed; Series-fed antennas; Series-feed patch array; Traveling-wave series-feed antenna array; Travelling waves; Iterative methods | English | 2023 | 2023-02 | 10.1109/tap.2022.3227751 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | An Inverse Agonist GSK5182 Increases Protein Stability of the Orphan Nuclear Receptor ERR? via Inhibition of Ubiquitination | The orphan nuclear receptor, estrogen-related receptor gamma (ERR gamma) is a constitutively active transcription factor involved in mitochondrial metabolism and energy homeostasis. GSK5182, a specific inverse agonist of ERR gamma that inhibits transcriptional activity, induces a conformational change in ERR gamma, resulting in a loss of coactivator binding. However, the molecular mechanism underlying the stabilization of the ERR gamma protein by its inverse agonist remains largely unknown. In this study, we found that GSK5182 inhibited ubiquitination of ERR gamma, thereby stabilizing the ERR gamma protein, using cell-based assays and confocal image analysis. Y326 of ERR gamma was essential for stabilization by GSK5182, as ligand-induced stabilization of ERR gamma was not observed with the ERR gamma-Y326A mutant. GSK5182 suppressed ubiquitination of ERR gamma by the E3 ligase Parkin and subsequent degradation. The inhibitory activity of GSK5182 was strong even when the ERR gamma protein level was elevated, as ERR gamma bound to GSK5182 recruited a corepressor, small heterodimer partner-interacting leucine zipper (SMILE), through the activation function 2 (AF-2) domain, without alteration of the nuclear localization or DNA-binding ability of ERR gamma. In addition, the AF-2 domain of ERR gamma was critical for the regulation of protein stability. Mutants in the AF-2 domain were present at higher levels than the wild type in the absence of GSK5182. Furthermore, the ERR gamma-L449A/L451A mutant was no longer susceptible to GSK5182. Thus, the AF-2 domain of ERR gamma is responsible for the regulation of transcriptional activity and protein stability by GSK5182. These findings suggest that GSK5182 regulates ERR gamma by a unique molecular mechanism, increasing the inactive form of ERR gamma via inhibition of ubiquitination. | Na, Soon-Young; Kim, Ki-Sun; Jung, Yoon Seok; Kim, Don-Kyu; Kim, Jina; Cho, Sung Jin; Lee, In-Kyu; Chung, Jongkyeong; Kim, Jeong-Sun; Choi, Hueng-Sik | Chonnam Natl Univ, Sch Biol Sci & Technol, Gwangju 61186, South Korea; Chonnam Natl Univ, Dept Integrat Food Biosci & Biotechnol, Gwangju 61186, South Korea; Daegu Gyeongbuk Med Innovat Fdn, New Drug Dev Ctr, Daegu 41061, South Korea; Korea Inst Sci & Technol, Brain Sci Inst, Ctr Brain Disorders, Seoul 02792, South Korea; Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Dept Internal Med, Daegu 41944, South Korea; Kyungpook Natl Univ, Res Inst Aging & Metab, Daegu 41940, South Korea; Seoul Natl Univ, Inst Mol Biol & Genet, SRC Ctr Syst Gerosci, Sch Biol Sci, Seoul 08826, South Korea; Chonnam Natl Univ, Dept Chem, Gwangju 61186, South Korea | Jung, Yoon/B-8512-2011; Kim, Sun/C-2026-2011; Lee, In-Kyu/AAR-6374-2021 | 7102024265; 57190437981; 57203348590; 37081358700; 56949261900; 58735369700; 36071537600; 56040953500; 7601379316; 7404338771 | hsc@chonnam.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 1 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0.27 | 2025-06-25 | 1 | 2 | nuclear receptor; ERR gamma; inverse agonist; protein stability; Parkin; ubiquitination | ESTROGEN-RELATED RECEPTORS; LIGAND-BINDING DOMAIN; 4-HYDROXYTAMOXIFEN ANALOGS; BIOLOGICAL EVALUATION; GAMMA; ALPHA; DEGRADATION; EXPRESSION; SMILE; ANTAGONISTS | ERRγ; inverse agonist; nuclear receptor; Parkin; protein stability; ubiquitination | Drug Inverse Agonism; Furylfuramide; Orphan Nuclear Receptors; Protein Stability; Ubiquitination; cycloheximide; estrogen receptor alpha; gsk 5182; heterodimer; parkin; progesterone receptor; protein synthesis inhibitor; unclassified drug; furylfuramide; GSK5182; orphan nuclear receptor; amino acid substitution; Article; cell lysate; cellular distribution; conformational transition; controlled study; crystal structure; DNA binding; human; hydrogen bond; image analysis; immunoblotting; immunohistochemistry; protein degradation; protein metabolism; protein stability; regulatory mechanism; transcription regulation; ubiquitination; Western blotting; inverse agonism; protein stability; ubiquitination | English | 2023 | 2023-01 | 10.3390/ijms24010096 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Anti-Inflammatory and Antioxidant Activities of Lipophilic Fraction from Liriope platyphylla Seeds Using Network Pharmacology, Molecular Docking, and In Vitro Experiments | Antioxidant and anti-inflammatory mechanisms counteract the pathogenesis of chronic diseases, such as diabetes, aging, and cancer. Therefore, enhancing antioxidant and anti-inflammatory functions may help manage these pathological conditions. This study aimed to assess the antioxidant and anti-inflammatory potentials of lipophilic fraction of Liriope platyphylla seeds (LLPS) using network pharmacology, molecular docking, and in vitro experiments. Here GC-MS analysis tentatively identified forty-three lipophilic compounds in LLPS. LLPS exhibited powerful antioxidant activity, according to the results from chemical-based antioxidant assays on DPPH, ABTS(+), superoxide anion, hydrogen peroxide, nitric oxide, and hydroxyl radicals scavenging, lipid peroxidation, reducing antioxidant powers, and total antioxidant capacity. Additionally, LLPS enhanced cellular antioxidant capacity by inhibiting reactive oxygen species formation and elevating antioxidant enzyme levels, including catalase and heme oxygenase-1. Moreover, LLPS attenuated inflammatory response by reducing nitric oxide secretion and downregulating the expression of inducible nitric oxide synthase, cyclooxygenase-2, and interleukin-1 beta in lipopolysaccharide-treated macrophages. Network pharmacology and molecular docking analyses showed that key compounds in LPPS, particularly phytosterols and fatty acid esters, exerted antioxidant and anti-inflammatory properties through regulating NFKB1, PTGS1, PTGS2, TLR4, PRKCA, PRKCD, KEAP1, NFE2L2, and NR1l2. Overall, these data suggest that LLPS may be a potential antioxidant and anti-inflammatory agent for developing functional foods. | Truong, Van-Long; Bae, Yeon-Ji; Rarison, Razanamanana H. G.; Bang, Ji-Hong; Park, So-Yoon; Jeong, Woo-Sik | Kyungpook Natl Univ, Coll Agr & Life Sci, Sch Food Sci & Biotechnol, Daegu 41566, South Korea; Kyungpook Natl Univ, Food & Bioind Res Inst, Sch Food Sci & Biotechnol, Coll Agr & Life Sci, Daegu 41566, South Korea | Jeong, Woo-Sik/AAN-6885-2020 | 55925363400; 58150149400; 57930282700; 58150149300; 58660979700; 10440750200 | truonglongpro@gmail.com;qoduswl530@naver.com;rhanitranirina@gmail.com;wlghd6780@naver.com;thdbs1958@naver.com;wsjeong@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 19 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 1.07 | 2025-06-25 | 8 | 9 | antioxidant; anti-inflammation; Liriope platyphylla seed; network pharmacology; molecular docking | NF-KAPPA-B; PROTEIN-KINASE; OXIDATIVE STRESS; FREE-RADICALS; INFLAMMATION; CYCLOARTENOL; HOMEOSTASIS; FUCOSTEROL; EXPRESSION; CELLS | anti-inflammation; antioxidant; Liriope platyphylla seed; molecular docking; network pharmacology | Anti-Inflammatory Agents; Antioxidants; Kelch-Like ECH-Associated Protein 1; Molecular Docking Simulation; Network Pharmacology; NF-E2-Related Factor 2; Nitric Oxide; Plant Extracts; catalase; cyclooxygenase 2; fatty acid ester; heme oxygenase 1; hydrocarbon; hypoxia inducible factor 1; I kappa B; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; interleukin 17; interleukin 1beta; kelch like ECH associated protein 1; lipopolysaccharide; mitogen activated protein kinase; nitric oxide; nucleotide binding oligomerization domain like receptor; peptides and proteins; phosphatidylinositol 3 kinase; phytosterol; protein kinase B; protein kinase C alpha; protein kinase C delta; protein nuclear receptor subfamily 1 group i member 2; protein prostaglandin endoperoxide synthase 1; protein prostaglandin endoperoxide synthase 2; reactive oxygen metabolite; terpene derivative; tocol; toll like receptor; toll like receptor 4; transcription factor Nrf2; tumor necrosis factor; unclassified drug; antiinflammatory agent; antioxidant; kelch like ECH associated protein 1; nitric oxide; plant extract; transcription factor Nrf2; ABTS radical scavenging assay; age rage signaling; antiinflammatory activity; antioxidant activity; antioxidant assay; Article; controlled study; down regulation; DPPH radical scavenging assay; gene ontology; hydrogen peroxide scavenging assay; hydroxyl radical scavenging assay; in vitro study; interleukin signaling; KEGG; lipid peroxidation assay; lipophilicity; Liriope (plant); Liriope platyphylla; macrophage; MAPK signaling; mass fragmentography; molecular docking; NF kB signaling; nitric oxide scavenging assay; nonhuman; Pi3K/Akt signaling; plant seed; protein expression; protein protein interaction; reducing antioxidant power assay; signal transduction; superoxide anion scavenging assay; systems pharmacology; TLR signaling; TNF signaling; total antioxidant capacity; metabolism | English | 2023 | 2023-10 | 10.3390/ijms241914958 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Antioxidant, Tyrosinase, α-Glucosidase, and Elastase Enzyme Inhibition Activities of Optimized Unripe Ajwa Date Pulp (Phoenix dactylifera) Extracts by Response Surface Methodology | The Ajwa date (Phoenix dactylifera L., Arecaceae family) is a popular edible fruit consumed all over the world. The profiling of the polyphenolic compounds of optimized unripe Ajwa date pulp (URADP) extracts is scarce. The aim of this study was to extract polyphenols from URADP as effectively as possible by using response surface methodology (RSM). A central composite design (CCD) was used to optimize the extraction conditions with respect to ethanol concentration, extraction time, and temperature and to achieve the maximum amount of polyphenolic compounds. High-resolution mass spectrometry was used to identify the URADP's polyphenolic compounds. The DPPH-, ABTS-radical scavenging, alpha-glucosidase, elastase and tyrosinase enzyme inhibition of optimized extracts of URADP was also evaluated. According to RSM, the highest amounts of TPC (24.25 +/- 1.02 mgGAE/g) and TFC (23.98 +/- 0.65 mgCAE/g) were obtained at 52% ethanol, 81 min time, and 63 degrees C. Seventy (70) secondary metabolites, including phenolic, flavonoids, fatty acids, and sugar, were discovered using high-resolution mass spectrometry. In addition, twelve (12) new phytoconstituents were identified for the first time in this plant. Optimized URADP extract showed inhibition of DPPH-radical (IC50 = 87.56 mg/mL), ABTS-radical (IC50 = 172.36 mg/mL), alpha-glucosidase (IC50 = 221.59 mg/mL), elastase (IC50 = 372.25 mg/mL) and tyrosinase (IC50 = 59.53 mg/mL) enzymes. The results revealed a significant amount of phytoconstituents, making it an excellent contender for the pharmaceutical and food industries. | Alshammari, Fanar; Alam, Md Badrul; Song, Bo-Rim; Lee, Sang-Han | Kyungpook Natl Univ, Grad Sch, Dept Food Sci & Biotechnol, Daegu 41566, South Korea; Kyungpook Natl Univ, Food & Bioind Res Inst, Inner Beauty Antiaging Ctr, Daegu 41566, South Korea | Alam, Md Badrul/AFL-7668-2022; Lee, Seung Eun/ABG-1607-2021 | 57191860948; 56706777100; 57223138662; 57221453703 | sang@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 4 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0.94 | 2025-06-25 | 7 | 7 | Ajwa date; enzymatic activity; polyphenolics; response surface methodology | ASSISTED EXTRACTION; L.; FRUIT; PALM; PREVENTION; CULTIVAR; DISEASES | Ajwa date; enzymatic activity; polyphenolics; response surface methodology | alpha-Glucosidases; Antioxidants; Monophenol Monooxygenase; Pancreatic Elastase; Phoeniceae; Plant Extracts; alcohol; alpha glucosidase; antioxidant; ascorbic acid; elastase; fatty acid; flavonoid; monophenol monooxygenase; phenolic acid; Phoenix dactylifera extract; phytochemical; plant extract; polyphenol derivative; solvent; sugar; unclassified drug; 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; alpha glucosidase; antioxidant; monophenol monooxygenase; pancreatic elastase; plant extract; ABTS radical scavenging assay; Ajwa; Arecaceae; Article; central composite design; chemometric analysis; comparative study; concentration (parameter); controlled study; date (fruit); DPPH radical scavenging assay; drug screening; drug structure; electrospray mass spectrometry; enzyme activity; enzyme inhibition; extraction time; IC50; mass spectrometry; metabolic fingerprinting; metabolite; nonhuman; response surface method; solvent extraction; temperature; validation study; chemistry; metabolism; Phoenix (plant) | English | 2023 | 2023-02 | 10.3390/ijms24043396 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Brevundimonas vesicularis (S1T13) Mitigates Drought-Stress-Associated Damage in Arabidopsis thaliana | Drought stress is a significant threat to agricultural productivity and poses challenges to plant survival and growth. Research into microbial plant biostimulants faces difficulties in understanding complicated ecological dynamics, molecular mechanisms, and specificity; to address these knowledge gaps, collaborative efforts and innovative strategies are needed. In the present study, we investigated the potential role of Brevundimonas vesicularis (S1T13) as a microbial plant biostimulant to enhance drought tolerance in Arabidopsis thaliana. We assessed the impact of S1T13 on Col-0 wild-type (WT) and atnced3 mutant plants under drought conditions. Our results revealed that the inoculation of S1T13 significantly contributed to plant vigor, with notable improvements observed in both genotypes. To elucidate the underlying mechanisms, we studied the role of ROS and their regulation by antioxidant genes and enzymes in plants inoculated with S1T13. Interestingly, the inoculation of S1T13 enhanced the activities of GSH, SOD, POD, and PPO by 33, 35, 41, and 44% in WT and 24, 22, 26, and 33% in atnced3, respectively. In addition, S1T13 upregulated the expression of antioxidant genes. This enhanced antioxidant machinery played a crucial role in neutralizing ROS and protecting plant cells from oxidative damage during drought stress. Furthermore, we investigated the impact of S1T13 on ABA and drought-stress-responsive genes. Similarly, S1T13 modulated the production of ABA and expression of AO3, ABA3, DREB1A, and DREB2A by 31, 42, 37, 41, and 42% in WT and 20, 29, 27, 38, and 29% in atnced3. The improvement in plant vigor, coupled with the induction of the antioxidant system and modulation of ABA, indicates the pivotal role of S1T13 in enhancing the drought stress tolerance of the plants. Conclusively, the current study provides valuable insights for the application of multitrait S1T13 as a novel strain to improve drought stress tolerance in plants and could be added to the consortium of biofertilizers. | Tran, Can Thi My; Al Azzawi, Tiba Nazar Ibrahim; Khan, Murtaza; Ali, Sajid; Moon, Yong-Sun; Yun, Byung-Wook | Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea; Yeungnam Univ, Dept Hort & Life Sci, Gyongsan 38541, South Korea | Ali, Sajid/GLS-7322-2022; Ali, Sajid/IWD-7100-2023 | 58750696800; 57224980187; 57207990116; 57214290889; 7203054923; 8245123600 | redflower660@yahoo.com;murtazakhan.bio@gmail.com;sajidbioali@gmail.com;hangulmys@ynu.ac.kr;bwyun@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 23 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0.13 | 2025-06-25 | 2 | 1 | drought stress; PGPRB; antioxidants; gene expression; ABA; Arabidopsis | SALICYLIC-ACID; PLANT-GROWTH | ABA; antioxidants; Arabidopsis; drought stress; gene expression; PGPRB | Abscisic Acid; Antioxidants; Arabidopsis; Arabidopsis Proteins; Brevundimonas vesicularis; Droughts; Gene Expression Regulation, Plant; Plant Proteins; Plants, Genetically Modified; Reactive Oxygen Species; Stress, Physiological; abscisic acid; chlorophyll a; chlorophyll b; electrolyte; glutathione; hydrogen peroxide; peroxidase; superoxide; superoxide dismutase; abscisic acid; antioxidant; Arabidopsis protein; plant protein; reactive oxygen metabolite; antioxidant activity; Arabidopsis thaliana; Article; Brevundimonas; Brevundimonas vesicularis; chlorophyll content; controlled study; drought stress; enzyme activity; gene expression; nonhuman; oxidative stress; plant cell; upregulation; Arabidopsis; drought; gene expression regulation; genetics; metabolism; physiological stress; transgenic plant | English | 2023 | 2023-12 | 10.3390/ijms242316590 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Characterization of the Seed Biopriming, Plant Growth-Promoting and Salinity-Ameliorating Potential of Halophilic Fungi Isolated from Hypersaline Habitats | Salinity stress is one of the major abiotic factors limiting crop yield in arid and semi-arid regions. Plant growth-promoting fungi can help plants thrive in stressful conditions. In this study, we isolated and characterized 26 halophilic fungi (endophytic, rhizospheric, and soil) from the coastal region of Muscat, Oman, for plant growth-promoting activities. About 16 out of 26 fungi were found to produce IAA, and about 11 isolates (MGRF1, MGRF2, GREF1, GREF2, TQRF4, TQRF5, TQRF5, TQRF6, TQRF7, TQRF8, TQRF2) out of 26 strains were found to significantly improve seed germination and seedling growth of wheat. To evaluate the effect of the above-selected strains on salt tolerance in wheat, we grew wheat seedlings in 150 mM, 300 mM NaCl and SW (100% seawater) treatments and inoculated them with the above strains. Our findings showed that fungal strains MGRF1, MGRF2, GREF2, and TQRF9 alleviate 150 mM salt stress and increase shoot length compared to their respective control plants. However, in 300 mM stressed plants, GREF1 and TQRF9 were observed to improve shoot length. Two strains, GREF2 and TQRF8, also promoted plant growth and reduced salt stress in SW-treated plants. Like shoot length, an analogous pattern was observed in root length, and different salt stressors such as 150 mM, 300 mM, and SW reduced root length by up to 4%, 7.5%, and 19.5%, respectively. Three strains, GREF1, TQRF7, and MGRF1, had higher catalase (CAT) levels, and similar results were observed in polyphenol oxidase (PPO), and GREF1 inoculation dramatically raised the PPO level in 150 mM salt stress. The fungal strains had varying effects, with some, such as GREF1, GREF2, and TQRF9, showing a significant increase in protein content as compared to their respective control plants. Under salinity stress, the expression of DREB2 and DREB6 genes was reduced. However, the WDREB2 gene, on the other hand, was shown to be highly elevated during salt stress conditions, whereas the opposite was observed in inoculated plants. | Aizaz, Muhammad; Ahmad, Waqar; Asaf, Sajjad; Khan, Ibrahim; Saad Jan, Syed; Salim Alamri, Safiya; Bilal, Saqib; Jan, Rahmatullah; Kim, Kyung-Min; Al-Harrasi, Ahmed | Univ Nizwa, Nat & Med Sci Res Ctr, Nizwa 616, Oman; Univ Houston, Dept Engn Technol, Sugar Land, TX 77479 USA; Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea | Ul-Hamid, Anwar/B-7297-2015; Khan, Ibrahim/JMC-5061-2023; Jan, Rahmatullah/AIC-3439-2022; Asaf, Sajjad/ABA-3647-2021; Kim, Kyung-Min Kim/C-7007-2014 | 57224641342; 59054664000; 56595059900; 57191379139; 58137940300; 58137044600; 57031617400; 57201981969; 34868260300; 6506093146 | kkm@knu.ac.kr;aharrasi@unizwa.edu.om; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 5 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 2.54 | 2025-06-25 | 18 | 20 | halophilic fungi; salinity; plant growth promotion; hormones; identification | VICIA-FABA L.; SALT-STRESS; PIRIFORMOSPORA-INDICA; ANTIOXIDANT ENZYMES; ENDOPHYTIC FUNGUS; ABIOTIC STRESS; TOLERANCE; ALLEVIATION; MECHANISMS; INOCULATION | halophilic fungi; hormones; identification; plant growth promotion; salinity | Ecosystem; Plant Development; Salinity; Seedlings; Seeds; catalase; catechol oxidase; flavonoid; flavonol; sea water; sodium chloride; Article; biomass; controlled study; dreb2 gene; dreb6 gene; dry weight; endophytic fungus; fresh weight; fungal strain; fungus identification; fungus isolation; gene; gene expression; germination; nonhuman; Oman; plant growth; plant seed; plant-growth promoting fungus; protein content; rhizosphere fungus; root length; salinity; salt stress; salt tolerance; shoot length; species habitat; wdreb2 gene; wheat; ecosystem; metabolism; plant development; plant seed; seedling | English | 2023 | 2023-03 | 10.3390/ijms24054904 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Dermatophagoides farinae Extract Induces Interleukin 33-Mediated Atopic Skin Inflammation via Activation of RIP1 | Receptor-interacting protein kinase (RIP) family 1 signaling has complex effects on inflammatory processes and cell death, but little is known concerning allergic skin diseases. We examined the role of RIP1 in Dermatophagoides farinae extract (DFE)-induced atopic dermatitis (AD)-like skin inflammation. RIP1 phosphorylation was increased in HKCs treated with DFE. Nectostatin-1, a selective and potent allosteric inhibitor of RIP1, inhibited AD-like skin inflammation and the expression of histamine, total IgE, DFE-specific IgE, IL-4, IL-5, and IL-13 in an AD-like mouse model. The expression of RIP1 was increased in ear skin tissue from a DFE-induced mouse model with AD-like skin lesions and in the lesional skin of AD patients with high house dust mite sensitization. The expression of IL-33 was down-regulated after RIP1 inhibition, and the levels of IL-33 were increased by over-expression of RIP1 in keratinocytes stimulated with DFE. Nectostatin-1 reduced IL-33 expression in vitro and in the DFE-induced mouse model. These results suggest that RIP1 can be one of the mediators that regulate IL-33-mediated atopic skin inflammation by house dust mites. | Jin, Meiling; Bang, Jin Seon; Ha, Dae-Lyong; Kim, Jun Young; Park, Kyung Duck; Lee, Weon Ju; Lee, Seok-Jong; Choi, Jin Kyeong; Choi, Young-Ae; Jang, Yong Hyun; Kim, Sang-Hyun | Yanbian Natl Univ, Sch Med, Dept Pharmacol, Yanji 133002, Peoples R China; Kyungpook Natl Univ, Sch Med, Dept Pharmacol, Daegu 41944, South Korea; Kyungpook Natl Univ, Biomed Res Inst, Sch Med, Dept Dermatol, Daegu 41944, South Korea; Jeonbuk Natl Univ, Med Sch, Dept Immunol, Jeonju 54907, South Korea | Lee, Joong/A-5417-2013 | 59808148000; 57781956500; 57201367090; 35310922800; 55767995700; 24474659000; 56013454400; 50261279600; 7404777420; 57016046400; 57210450420 | yhjang@knu.ac.kr;shkim72@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 6 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0 | 2025-06-25 | 0 | 0 | atopic dermatitis; Dermatophagoides farinae; house dust mite; interleukin-33; receptor-interacting protein kinase 1 | INNATE; CYTOKINES; IL-33 | atopic dermatitis; Dermatophagoides farinae; house dust mite; interleukin-33; receptor-interacting protein kinase 1 | Animals; Antigens, Dermatophagoides; Cytokines; Dermatitis, Atopic; Dermatophagoides farinae; Disease Models, Animal; Immunoglobulin E; Inflammation; Interleukin-33; Mice; Plant Extracts; Pyroglyphidae; Skin; dust mite extract; histamine; immunoglobulin E; interleukin 13; interleukin 33; interleukin 4; interleukin 5; phosphotransferase; protein kinase; receptor interacting protein kinase 1; unclassified drug; cytokine; house dust allergen; immunoglobulin E; interleukin 33; plant extract; Rip11 protein, mouse; allosterism; animal experiment; animal model; animal tissue; Article; atopic dermatitis; cell infiltration; complex formation; controlled study; dermatitis; Dermatophagoides; Dermatophagoides farinae; down regulation; ear thickness; ear tissue; enzyme activation; female; gene expression; gene overexpression; HaCat cell line; human; human cell; human tissue; in vitro study; innate immunity; keratinocyte; mouse; nonhuman; protein expression; protein function; protein phosphorylation; sensitization; skin defect; animal; disease model; inflammation; pathology; Pyroglyphidae; skin | English | 2023 | 2023-03 | 10.3390/ijms24065228 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Detection and Genotypic Analysis of Anaplasma bovis and A. phagocytophilum in Horse Blood and Lung Tissue | A clinical case of Anaplasma bovis was reported for the first time in our previous study (2019) in a horse, a nondefinitive host. Although A. bovis is a ruminant and not a zoonotic pathogen, it is responsible for persistent infections in horses. In this follow-up study, the prevalence of Anaplasma spp., including A. bovis, was assessed in horse blood and lung tissue samples to fully understand Anaplasma spp. pathogen distribution and the potential risk factors of infection. Among 1696 samples, including 1433 blood samples from farms nationwide and 263 lung tissue samples from horse abattoirs on Jeju Island, a total of 29 samples (1.7%) tested positive for A. bovis and 31 (1.8%) samples tested positive for A. phagocytophilum, as determined by 16S rRNA nucleotide sequencing and restriction fragment length polymorphism. This study is the first to detect A. bovis infection in horse lung tissue samples. Further studies are needed to clarify the comparison of sample types within cohorts. Although the clinical significance of Anaplasma infection was not evaluated in this study, our results emphasize the need to clarify the host tropism and genetic divergence of Anaplasma to enable the development of effective prevention and control measures through broad epidemiological studies. | Seo, Min-Goo; Ouh, In-Ohk; Kwak, Dongmi | Kyungpook Natl Univ, Coll Vet Med, 80 Daehak ro, Daegu 41566, South Korea; Korea Dis Control & Prevent Agcy, Natl Inst Hlth, 212 Osongsaengmyeong 2 ro, Cheongju 28160, Chungbuk, South Korea | ; Seo, Min-Goo/NQF-4335-2025 | 53982155300; 56096229500; 7007148758 | dmkwak@knu.ac.kr; | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | INT J MOL SCI | 1661-6596 | 1422-0067 | 24 | 4 | SCIE | BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY | 2023 | 4.9 | 20.9 | 0.67 | 2025-06-25 | 4 | 5 | Anaplasma spp; blood; horse; phylogeny; lung; restriction fragment length polymorphism | MOLECULAR-DETECTION; PHYLOGENETIC ANALYSIS; INFECTION; IDENTIFICATION; CATTLE; EHRLICHIA; MARGINALE; GOATS; RICKETTSIALES; VARIANTS | Anaplasmaspp; blood; horse; lung; phylogeny; restriction fragment length polymorphism | Anaplasma; Anaplasmosis; Animals; DNA, Bacterial; Follow-Up Studies; Horses; Phylogeny; RNA, Ribosomal, 16S; Ruminants; nucleotide; RNA 16S; bacterial DNA; RNA 16S; agricultural land; Anaplasma phagocytophilum; Article; blood sampling; clinical significance; comparative study; DNA extraction; DNA sequencing; Escherichia coli; follow up; genetic variability; horse; host pathogen interaction; host tropism; housekeeping gene; human; lung parenchyma; maximum likelihood method; molecular cloning; nested polymerase chain reaction; nonhuman; polymerase chain reaction; prevention and control; raccoon dog; restriction fragment length polymorphism; sample size; slaughterhouse; species identification; Anaplasma; anaplasmosis; animal; genetics; horse; phylogeny; ruminant | English | 2023 | 2023-02 | 10.3390/ijms24043239 | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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