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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | ○ | Article | Diagnostic yield and clinical utility of whole exome sequencing using an automated variant prioritization system,EVIDENCE | EVIDENCE, an automated variant prioritization system, has been developed to facilitate whole exome sequencing analyses. This study investigated the diagnostic yield of EVIDENCE in patients with suspected genetic disorders. DNA from 330 probands (age range, 0-68 years) with suspected genetic disorders were subjected to whole exome sequencing. Candidate variants were identified by EVIDENCE and confirmed by testing family members and/or clinical reassessments. EVIDENCE reported a total 228 variants in 200 (60.6%) of the 330 probands. The average number of organs involved per patient was 4.5 +/- 5.0. After clinical reassessment and/or family member testing, 167 variants were identified in 141 probands (42.7%), including 105 novel variants. These variants were confirmed as being responsible for 121 genetic disorders. A total of 103 (61.7%) of the 167 variants in 95 patients were classified as pathogenic or probably to be pathogenic before, and 161 (96.4%) variants in 137 patients (41.5%) after, clinical assessment and/or family member testing. Factor associated with a variant being regarded as causative includes similar symptom scores of a gene variant to the phenotype of the patient. This new, automated variant interpretation system facilitated the diagnosis of various genetic diseases with a 42.7% diagnostic yield. | Seo, Go Hun; Kim, Taeho; Choi, In Hee; Park, Jung-young; Lee, Jungsul; Kim, Sehwan; Won, Dhong-gun; Oh, Arum; Lee, Yena; Choi, Jeongmin; Lee, Hajeong; Kang, Hee Gyung; Cho, Hee Yeon; Cho, Min Hyun; Kim, Yoon Jeon; Yoon, Young Hee; Eun, Baik-Lin; Desnick, Robert J.; Keum, Changwon; Lee, Beom Hee | 3billion Inc, Div Med Genet, Seoul, South Korea; Univ Ulsan, Asan Inst Life Sci, Biomed Res Ctr, Coll Med, Seoul, South Korea; Univ Ulsan, Med Genet Ctr, Asan Med Ctr, Coll Med, Seoul, South Korea; Univ Ulsan, Dept Pediat, Asan Med Ctr, Coll Med, Seoul, South Korea; Seoul Natl Univ, Seoul Natl Univ Hosp, Dept Internal Med, Coll Med, Seoul, South Korea; Seoul Natl Univ, Seoul Natl Univ Hosp, Dept Pediat, Coll Med, Seoul, South Korea; Sungkyunkwan Univ, Dept Pediat, Samsung Med Ctr, Sch Med, Seoul, South Korea; Kyungpook Natl Univ, Sch Med, Dept Pediat, Daegu, South Korea; Univ Ulsan, Dept Ophthalmol, Asan Med Ctr, Seoul, South Korea; Korea Univ, Dept Pediat, Coll Med, Seoul, South Korea; Icahn Sch Med Mt Sinai, Mt Sinai Med Ctr, Dept Genet & Genom Sci, New York, NY 10029 USA | ; Kim, Yoon Jeon/GZA-3765-2022; Lee, Jungsul/A-7851-2013 | 57062033100; 59442794700; 55513671300; 57219005374; 56050008300; 57219011361; 57219014558; 57210467812; 57218133973; 57215203693; 36554389800; 7404071546; 15724272900; 7401727726; 35761498000; 16204492800; 6602326137; 35407620100; 57210465047; 55513175800 | bhlee@amc.seoul.kr; | CLINICAL GENETICS | CLIN GENET | 0009-9163 | 1399-0004 | 98 | 6 | SCIE | GENETICS & HEREDITY | 2020 | 4.438 | 30.4 | 4.52 | 2025-06-25 | 111 | 111 | automated prioritization system; genetic diagnosis; variant; whole exome sequencing | GENOME; GENETICS; IDENTIFICATION; DISCOVERY; INFANTS | automated prioritization system; genetic diagnosis; variant; whole exome sequencing | Adolescent; Adult; Aged; Automation; Child; Child, Preschool; Computational Biology; Databases, Genetic; Exome; Female; Genetic Diseases, Inborn; Genetic Variation; Humans; Infant; Infant, Newborn; Male; Middle Aged; Phenotype; Whole Exome Sequencing; Young Adult; genomic DNA; adolescent; adult; aged; Article; child; clinical classification; controlled study; diagnostic value; family assessment; female; genetic disorder; genetic screening; genetic variation; human; indel mutation; infant; major clinical study; male; newborn; ontology; phenotype; pilot study; priority journal; single nucleotide polymorphism; symptom; whole exome sequencing; automation; biology; classification; exome; genetic database; genetic disorder; genetic variation; genetics; middle aged; pathology; preschool child; young adult | English | 2020 | 2020-12 | 10.1111/cge.13848 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Factors affecting the dormancy and germination of bleeding heart [Lamprocapnos spectabilis (L.) Fukuhara] seeds | Information on the optimal conditions to promote the germination of Lamprocapnos spectabilis (L.) Fukuhara seeds is limited; consequently, this study was conducted to establish the requirements to break seed dormancy and promote germination. The selected seeds had morphophysiological dormancy and had not begun embryo development. To study the dormancy breaking and embryo development processes, seeds were subjected to constant or changing temperature treatments during moist stratification. High temperature and humidity resulted in vigorous embryo growth, with the longest embryos occurring after 1 month of incubation at 20 degrees C. At 4 degrees C, the seeds required incubation period of at least 3 months to germinate. Embryo growth and germination were higher with changing high and low temperatures than under a constant temperature, and changing temperatures also considerably changed the endogenous hormone levels, embryo development and germination. Bioactive gibberellin (GA) content was higher in seeds incubated at 20 degrees C for 1 month, then at 4 degrees C for 2 months. The content of endogenous abscisic acid in seeds subjected to the same treatment decreased by 97.6% compared with that of the untreated seeds. Embryo growth and seed germination require changing high and low temperatures; however, exogenous GA(3) could substitute for high temperatures, as it also causes accelerated germination. In this study, the seeds of L. spectabilis were identified as an intermediate simple type, a sub-level of morphophysiologically dormant seeds. | Cho, J. S.; Jang, B. K.; Lee, S. M.; Lee, I. J.; Lee, C. H. | Chungbuk Natl Univ, Sch Appl Plant Sci & Biotechnol, Major Hort, Cheongju, South Korea; Chungbuk Natl Univ, Brain Korea Ctr Bioresource Dev 21, Cheongju 28644, South Korea; Gyeongsangnam Do Agr Res & Extens Serv, Environm Agr Res Div, Jinju, South Korea; Kyungpook Natl Univ, Sch Appl Biosci, Daegu, South Korea | Lee, In-Jung/GLS-0432-2022 | 57189053294; 57203308403; 56735697700; 16425830900; 56100698800 | leech@chungbuk.ac.kr; | PLANT BIOLOGY | PLANT BIOLOGY | 1435-8603 | 1438-8677 | 22 | 3 | SCIE | PLANT SCIENCES | 2020 | 3.081 | 30.4 | 0.52 | 2025-06-25 | 6 | 6 | Abscisic acid; embryo development; gibberellins; Lamprocapnos spectabilis; moisture stratification; morphophysiological dormancy | ABSCISIC-ACID; EMBRYO DEVELOPMENT; TEMPERATURE; BIOSYNTHESIS; STRATIFICATION; GIBBERELLINS; METABOLISM; GROWTH | Abscisic acid; embryo development; gibberellins; Lamprocapnos spectabilis; moisture stratification; morphophysiological dormancy | Abscisic Acid; Germination; Gibberellins; Papaveraceae; Plant Dormancy; Seeds; Temperature; Lamprocapnos spectabilis; abscisic acid; gibberellin; embryonic development; environmental factor; germination; seed dormancy; stratification; temperature effect; chemistry; dormancy; germination; metabolism; Papaveraceae; physiology; plant seed; temperature | English | 2020 | 2020-05 | 10.1111/plb.13089 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Plant growth-promoting endophytic bacteria augment growth and salinity tolerance in rice plants | Salt stress negatively affects growth and development of plants. However, it is hypothesized that plant growth-promoting endophytic bacteria can greatly alleviate the adverse effects of salinity and can promote growth and development of plants. In the present research, we aimed to isolate endophytic bacteria from halotolerant plants and evaluate their capacity for promoting crop plant growth. The bacterial endophytes were isolated from selected plants inhabiting sand dunes at Pohang beach, screened for plant growth-promoting traits and applied to rice seedlings under salt stress (NaCl; 150 mm). Out of 59 endophytic bacterial isolates, only six isolates,i.e. Curtobacterium oceanosedimentumSAK1,Curtobacterium luteumSAK2,Enterobacter ludwigiiSAK5,Bacillus cereusSA1,Micrococcus yunnanensisSA2,Enterobacter tabaciSA3, resulted in a significant increase in the growth ofWaito-Crice. The cultural filtrates of bacterial endophytes were tested for phytohormones, including indole-3-acetic acid, gibberellins and organic acids. Inoculation of the selected strains considerably reduced the amount of endogenous ABA in rice plants under NaCl stress, however, they increased GSH and sugar content. Similarly, these strains augmented the expression of flavin monooxygenase (OsYUCCA1) and auxin efflux carrier (OsPIN1) genes under salt stress. In conclusion, the pragmatic application of the above selected bacterial strains alleviated the adverse effects of NaCl stress and enhanced rice growth attributes by producing various phytohormones. | Khan, M. A.; Asaf, S.; Khan, A. L.; Adhikari, A.; Jan, R.; Ali, S.; Imran, M.; Kim, K-M; Lee, I-J | Kyungpook Natl Univ, Sch Appl Biosci, Daegu 41566, South Korea; Univ Nizwa, Nat & Med Sci Res Ctr, Nizwa, Oman | ; Jan, Rahmatullah/AIC-3439-2022; Adhikari, Arjun/JCO-3306-2023; Khan, Abdul/D-9215-2017; Ali, Sajid/GLS-7322-2022; Imran, Muhammad/AFL-6590-2022; Adhikari, Arjun/AAV-6297-2021; Khan, Muhammad/ABB-9797-2021; Asaf, Sajjad/ABA-3647-2021; Kim, Kyung-Min Kim/C-7007-2014; Lee, In-Jung/GLS-0432-2022 | 57188585606; 56595059900; 26639372800; 57195601415; 57201981969; 57214290889; 58282433800; 34868260300; 16425830900 | ijlee@knu.ac.kr; | PLANT BIOLOGY | PLANT BIOLOGY | 1435-8603 | 1438-8677 | 22 | 5 | SCIE | PLANT SCIENCES | 2020 | 3.081 | 30.4 | 7.44 | 2025-06-25 | 111 | 113 | Bacterial endophytes; gene expression; phytohormones; rice plant; salinity stress | IAA PRODUCING BACTERIA; SPHINGOMONAS SP LK11; INDOLE ACETIC-ACID; SALT-STRESS; AUXIN BIOSYNTHESIS; ACC-DEAMINASE; INDOLE-3-ACETIC-ACID IAA; AZOSPIRILLUM-BRASILENSE; PHYTOHORMONE PRODUCTION; PHASEOLUS-VULGARIS | Bacterial endophytes; gene expression; phytohormones; rice plant; salinity stress | Actinobacteria; Bacterial Physiological Phenomena; Endophytes; Enterobacter; Micrococcus; Oryza; Plant Roots; Salt Tolerance; North Kyongsang; Pohang; South Korea; Bacillus cereus; Bacteria (microorganisms); Curtobacterium; Curtobacterium luteum; Enterobacter; Enterobacter ludwigii; Micrococcus; bacterium; concentration (composition); crop plant; endophyte; phytohormone; rice; salinity tolerance; seedling; sugar; Actinobacteria; bacterial phenomena and functions; endophyte; Enterobacter; microbiology; Micrococcus; Oryza; physiology; plant root; salt tolerance | English | 2020 | 2020-09 | 10.1111/plb.13124 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Regularized Exploratory Bifactor Analysis With Small Sample Sizes | Several methods of factor extraction have recently gained popularity as a procedure for dealing with estimation problems associated with small sample sizes, which can be found in the various behavioral science disciplines, such as comparative psychology and behavior genetics. Two popular approaches for particularly small samples (below 50) include unweighted least squares factor analysis (ULS-FA) and regularized exploratory factor analysis (REFA). However, it is unclear how well each of the approaches performs with small samples in the context of exploratory bifactor modeling. In the current study, a comprehensive simulation study was conducted to evaluate the small sample behavior of the two approaches in terms of bifactor structure recovery under different sample size, factor loading, number of variables per factor, number of factors, and factor correlation experimental conditions. The results show that REFA is recommended for use over ULS-FA, particularly in the conditions involving low factor loadings, few group factors, or a small number of variables per factor. | Jung, Sunho; Seo, Dong Gi; Park, Jungkyu | Kyung Hee Univ, Sch Management, Seoul, South Korea; Hallym Univ, Dept Psychol, Chunchon, South Korea; Kyungpook Natl Univ, Dept Psychol, Daegu, South Korea | Jung, Sunho/AAF-9467-2021 | 35147888400; 57132031600; 56206565000 | wmotive@hallym.ac.kr;jkp@knu.ac.kr; | FRONTIERS IN PSYCHOLOGY | FRONT PSYCHOL | 1664-1078 | 11 | SSCI | PSYCHOLOGY, MULTIDISCIPLINARY | 2020 | 2.988 | 30.4 | 0.49 | 2025-06-25 | 13 | 18 | exploratory bifactor analysis; small sample size; unweighted least squares; regularized exploratory factor analysis; Monte Carlo simulation | SCHMID-LEIMAN ORTHOGONALIZATION; PERSONALITY; ROTATION; ALGORITHMS; MODELS | exploratory bifactor analysis; Monte Carlo simulation; regularized exploratory factor analysis; small sample size; unweighted least squares | English | 2020 | 2020-04-09 | 10.3389/fpsyg.2020.00507 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Coordinated corrective control for model matching of asynchronous sequential machines | This paper proposes coordinated corrective control of input/state asynchronous sequential machines (ASMs). The states of the considered ASM are physically or logically separated to which complete observation by a single controller is impossible. A number of local dynamic controllers that have access to local states cooperate to match the stable-state behaviour of the closed-loop system to that of a reference model. We investigate reachability description of ASMs in the configuration of coordinated control and present the existence condition and design procedure for local controllers that solve the model matching problem. An illustrative example is provided to validate the proposed coordinated corrective control scheme. | Yang, Jung-Min; Lee, Dong-Eun; Park, Seong-Jin | Kyungpook Natl Univ, Sch Elect Engn, Daegu, South Korea; Kyungpook Natl Univ, Sch Architecture & Civil Engn, Daegu, South Korea; Ajou Univ, Dept Elect & Comp Engn, 206 Worldcup Ro, Suwon 16499, South Korea | 57208450551; 56605563300; 57188837512 | parksjin@ajou.ac.kr; | INTERNATIONAL JOURNAL OF SYSTEMS SCIENCE | INT J SYST SCI | 0020-7721 | 1464-5319 | 51 | 15 | SCIE | AUTOMATION & CONTROL SYSTEMS;COMPUTER SCIENCE, THEORY & METHODS;OPERATIONS RESEARCH & MANAGEMENT SCIENCE | 2020 | 2.281 | 30.5 | 0 | 2025-06-25 | 0 | 0 | Asynchronous sequential machines (ASMs); dynamic corrective control; coordinated control; model matching | INPUT/OUTPUT CONTROL | Asynchronous sequential machines (ASMs); coordinated control; dynamic corrective control; model matching | Closed loop systems; Controllers; Electric machine theory; Sequential machines; Asynchronous sequential machines; Co-ordinated control; Corrective control; Existence conditions; Local controllers; Model matching problems; Reference modeling; Single controllers; Electric machine control | English | 2020 | 2020-11-17 | 10.1080/00207721.2020.1803440 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | In-Band RCS Reduction and Isolation Enhancement of a 24 GHz Radar Antenna Using Metamaterial Absorber for Sensing and Automotive Radar Applications | A triple-band low radar cross-section (RCS) high isolation antenna is proposed for 24 GHz ISM band sensing and automotive radar applications. The proposed design consists of 2 x 2 patch array that acts as transmit and receive antennas. Low RCS and high isolation are achieved at 24 GHz by designing a metamaterial absorber (MA), which consists of a square ring with a resistor connected in its diagonal arm for the absorption of electromagnetic waves. The proposed MA shows near unity normalized impedance at 24.1 GHz with 90% absorptivity bandwidth of 1 GHz. An array of MA is placed in between and around the transmit/receive antennas to suppress surface current and reduce in-band RCS of the radar sensor. The -10 dB impedance bandwidths of the triple-band sensor antenna are 20.8 to 21.24 GHz, 23.94 to 24.55 GHz, and 27.18 to 27.5 GHz. The proposed sensor antenna achieves isolation of 34 dB between the transmit and receive ports, and peak RCS reduction of 11 dB, as compared to the reference antenna. The half-power beamwidth of the proposed sensor antenna is 38 degrees for E-plane and 52 degrees for H-plane at 24 GHz. | Sharma, Ankit; Dwari, Santanu; Kanaujia, Binod Kumar; Gangwar, Deepak; Kumar, Sachin; Singh, Satya P.; Lay-Ekuakille, Aime | Indian Inst Technol ISM Dhanbad, Dept Elect Engn, Dhanbad 826004, Bihar, India; Galgotias Coll Engn & Technol, Greater Noida 201310, India; Indian Inst Technol ISM, Dept Elect Engn, Dhanbad 826004, Bihar, India; Jawaharlal Nehru Univ, Sch Computat & Integrat Sci, New Delhi 110067, India; Bharati Vidhyapeeths Coll Engn, Dept Elect & Commun Engn, New Delhi 416001, India; Kyungpook Natl Univ, Sch Elect Engn, Daegu 41566, South Korea; Nanyang Technol Univ, Sch Comp Sci & Engn, Singapore 639798, Singapore; Univ Salento, Dept Innovat Engn, I-73100 Lecce, Italy | ; Dwari, Santanu/NQF-2967-2025; Kanaujia, Binod/L-6484-2019; Singh, Satya P/AAI-2932-2020; Kumar, Sachin/W-2211-2019; Sharma, Ankit/AAD-1183-2019; Lay-Ekuakille, Aime'/AFN-8262-2022; Gangwar, Deepak/I-8876-2019; kanaujia, Binod/L-6484-2019 | 55605770334; 13205077400; 56962785800; 55258945300; 56907994000; 56175904100; 6602987304 | ankit.deli@gmail.com;santanu@iitism.ac.in;bkkanaujia@yahoo.co.in;er.deepakgangwar@gmail.com;gupta.sachin0708@gmail.com;satya@ntu.edu.sg;aime.lay.ekuakille@unisalento.it; | IEEE SENSORS JOURNAL | IEEE SENS J | 1530-437X | 1558-1748 | 20 | 21 | SCIE | ENGINEERING, ELECTRICAL & ELECTRONIC;INSTRUMENTS & INSTRUMENTATION;PHYSICS, APPLIED | 2020 | 3.301 | 30.5 | 1.51 | 2025-06-25 | 24 | 33 | Radar antennas; Radar cross-sections; Antenna arrays; Sensor arrays; Antenna array; automotive radar sensor; high gain; low RCS; metamaterial absorber (MA); mutual coupling | SENSOR; ARRAY | Antenna array; automotive radar sensor; high gain; low RCS; metamaterial absorber (MA); mutual coupling | Antenna arrays; Automotive radar; Bandwidth; Directional patterns (antenna); Electric impedance; Metamaterial antennas; Metamaterials; Radar antennas; Radar cross section; Receiving antennas; Slot antennas; Automotive radar applications; Half power beamwidth; High isolation antennas; Impedance bandwidths; Isolation enhancement; Metamaterial absorbers; Normalized impedance; Reference antennas; Microwave antennas | English | 2020 | 2020-11-01 | 10.1109/jsen.2020.3002337 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | The Impact of Moderate-Dose Acetylsalicylic Acid in the Reduction of Inflammatory Cytokine and Prevention of Complication in Acute Phase of Kawasaki Disease: The Benefit of Moderate-Dose Acetylsalicylic Acid | Background: Acetylsalicylic acid (ASA) is part of the recommended treatment of Kawasaki disease (KD). Controversies remain regarding the optimal dose of ASA. We aimed to evaluate the impact of different doses of ASA on inflammation control while minimizing adverse effects in the acute phase treatment of KD. Methods: The enrolled 323 patients with KD were divided into three groups according to ASA dose: moderate-dose (30-50 mg/kg/day), high-dose (80-100 mg/kg/day), and non-ASA. Results: High-dose ASA group showed a significantly shorter duration of fever from the start of treatment to remission than other groups. Baseline level and delta score of interleukin (IL)-1, IL-6, IL-10, tumor necrosis factor-alpha, and transforming growth factor beta were not statistically different among the groups. The number of patients who received additional treatments in the non-ASA group was more than other groups. Coronary artery dilatation was not significantly different among the groups. One patient with high-dose ASA was diagnosed with Reye syndrome. Conclusion: Different doses of ASA did not show any differences in changes of inflammatory bio-makers and cytokines. However, high-dose ASA showed occurrence of Reye syndrome, and non-ASA showed intravenous immunoglobulin refractoriness. We suggest that moderate-dose ASA may be beneficial for the treatment of patients in the acute phase of KD. | Kwon, Jung Eun; Roh, Da Eun; Kim, Yeo Hyang | Kyungpook Natl Univ, Kyungpook Natl Univ Childrens Hosp, Sch Med, Div Pediat Cardiol,Dept Pediat, Daegu 41404, South Korea | 57194002366; 57195734230; 57032023800 | lovecello623@gmail.com;ponyks1004@naver.com;kimyhmd@knu.ac.kr; | CHILDREN-BASEL | CHILDREN-BASEL | 2227-9067 | 7 | 10 | SCIE | PEDIATRICS | 2020 | 2.863 | 30.6 | 0.43 | 2025-06-25 | 6 | 6 | acetylsalicylic acid; coronary artery; fever; Kawasaki disease | INTRAVENOUS IMMUNOGLOBULIN; MANAGEMENT; ARTERITIS; PROFILES; GLOBULIN; ASPIRIN; TNF | Acetylsalicylic acid; Coronary artery; Fever; Kawasaki disease | English | 2020 | 2020-10 | 10.3390/children7100185 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Causal mediation analysis in nested case-control studies using conditional logistic regression | The paper proposes an approach to causal mediation analysis in nested case-control study designs, often incorporated with countermatching schemes using conditional likelihood, and we compare the method's performance to that of mediation analysis using the Cox model for the full cohort with a continuous or dichotomous mediator. Simulation studies are conducted to assess our proposed method and investigate the efficiency relative to the cohort. We illustrate the method using actual data from two studies of potential mediation of radiation risk conducted within the Adult Health Study cohort of atomic-bomb survivors. The performance becomes comparable to that based on the full cohort, illustrating the potential for valid mediation analysis based on the reduced data obtained through the nested case-control design. | Kim, Young Min; Cologne, John B.; Jang, Euna; Lange, Theis; Tatsukawa, Yoshimi; Ohishi, Waka; Utada, Mai; Cullings, Harry M. | Kyungpook Natl Univ, Dept Stat, 410 Coll Nat Sci Bldg,80 Daehak Ro, Daegu 41566, South Korea; Radiat Effects Res Fdn, Dept Stat, Hiroshima, Hiroshima, Japan; Univ Copenhagen, Sect Biostat, Copenhagen, Denmark; Radiat Effects Res Fdn, Dept Clin Studies, Hiroshima, Hiroshima, Japan; Radiat Effects Res Fdn, Dept Epidemiol, Hiroshima, Hiroshima, Japan | ; Utada, Mai/KDN-8907-2024 | 56035273800; 7004075047; 57217388673; 37031450700; 8532182700; 6603185673; 36705155300; 35067866700 | kymmyself@knu.ac.kr; | BIOMETRICAL JOURNAL | BIOMETRICAL J | 0323-3847 | 1521-4036 | 62 | 8 | SCIE | MATHEMATICAL & COMPUTATIONAL BIOLOGY;STATISTICS & PROBABILITY | 2020 | 2.207 | 30.8 | 0.73 | 2025-06-25 | 10 | 8 | causal mediation analysis; cohort; conditional logistic regression; Cox proportional hazards model; nested case-control study | ATOMIC-BOMB SURVIVORS; BREAST-CANCER; RADIATION; IMPLEMENTATION; STATISTICS; EFFICIENCY; RISK | causal mediation analysis; cohort; conditional logistic regression; Cox proportional hazards model; nested case-control study | Logistic regression; Case-control study; Causal mediation analyse; Cohort; Conditional likelihood; Conditional logistic regressions; Cox proportional hazard model; Mediation analysis; Nested case-control study; Performance; Study design; adult; article; atomic bomb survivor; case control study; cohort analysis; controlled study; female; human; male; radiation hazard; simulation; Health risks | English | 2020 | 2020-12 | 10.1002/bimj.201900120 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Electrical and carrier transport properties of Au/Pr6O11/n-GaN MIS structure with a high-k rare-earth oxide interlayer at high temperature range | The electrical and carrier transport properties of Au/Pr6O11/n-GaN metal/interlayer/semiconductor (MIS) structure with an electron beam deposited high-k rare-earth oxide as an interlayer have been investigated in the temperature range of 270-420 K. The electrical characteristics such as current-voltage (I-V), turn-on voltage and series resistance (R-S) are analyzed at different temperatures. These analysis showed that the obtained barrier heights (Phi(b)) increase while ideality factor (n) and R-S decrease with increasing temperature. The characteristic temperature (T-0) is determined from the R-S against temperature plots, and the value (T-0) is well matched with each other. The calculated thermal coefficient (K-j) value is - 2.1 mV/K at >= 270 K. The obtained effective barrier height (1.22 eV) is consistent with the theoretical value. The temperature-dependent ideality factor values are in good agreement with characteristic energy of 31 meV. The estimated interface state density (N-SS) increases with decreasing temperature that could be associated with the molecular restructuring and reordering of the Pr6O11/n-GaN interface. The reverse bias results showed that the current conduction is dominated by Poole-Frenkel emission in the temperature range of 270-330 K while the Schottky emission is dominated in the temperature range of 360-420 K. | Uma, M.; Reddy, M. Siva Pratap; Reddy, K. Ravindranatha; Reddy, V. Rajagopal | Sri Venkateswara Univ, Dept Phys, Tirupati 517502, Andhra Pradesh, India; Kyungpook Natl Univ, Sch Elect Engn, Daegu 41566, South Korea; SGS Arts Coll, Dept Phys, Tirupati 517502, Andhra Pradesh, India | Umar, Moh. Tauhid/U-6055-2017; V, Rajagopal Reddy/AFK-1413-2022 | 57208260574; 6504345678; 57200075078; 58070251100 | dr.mspreddy@gmail.com;reddy_vrg@rediffmail.com; | VACUUM | VACUUM | 0042-207X | 174 | SCIE | MATERIALS SCIENCE, MULTIDISCIPLINARY;PHYSICS, APPLIED | 2020 | 3.627 | 30.9 | 0.84 | 2025-06-25 | 13 | 13 | Rare-earth oxide; Pr6O11 interlayer; n(Si-doped)-GaN; Carrier transport; Thermal coefficient | C-V CHARACTERISTICS; METAL-SEMICONDUCTOR; CONDUCTION MECHANISMS; INTERFACE STATES; SCHOTTKY DIODES; BARRIER HEIGHT; I-V; GOLD | Carrier transport; n(Si-doped)-GaN; Pr<sub>6</sub>O<sub>11</sub> interlayer; Rare-earth oxide; Thermal coefficient | Carrier transport; Electric resistance; Gallium nitride; Gold deposits; III-V semiconductors; Interface states; Rare earths; Silicon compounds; Temperature; Transport properties; Characteristic temperature; Effective barrier heights; Electrical characteristic; Increasing temperatures; Molecular restructuring; Pr6O11 interlayer; Rare earth oxide; Thermal coefficients; Praseodymium compounds | English | 2020 | 2020-04 | 10.1016/j.vacuum.2020.109201 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Glibenclamide modulates microglial function and attenuates Aβ deposition in 5XFAD mice | Severe neuroinflammation is known as a main pathology of neurodegenerative disorders such as Alzheimer's disease (AD) and Parkinson's disease (PD). In these diseases, excessive microglial activation is one of the main causes of inflammation in the central nervous system. Therefore, inhibition of activated microglia may be suggested as a treatment for neuminflammatory diseases. Glibenclamide, known as a therapeutics for type 2 diabetes in clinical trials has been shown to be effective in the inhibiting inflammatory conditions of various diseases. However, studies on the effects of glibenclamide for improving AD pathologies are little known. In this study, we tested glibenclamide on microglial cell line BV2 and 5XFAD mice. We found that glibenclamide significantly inhibited nitric oxide (NO) at 10 mu M and 40 mu M in BV2 cells induced by lipopolysaccharide (LPS) stimulation. In addition, we confirmed that 40 mu M of glibenclamide reduced pro-inflammatory cytokines and proteins in the LPS-stimulated microglial cells. The anti-inflammatory effect of glibenclamide was further tested in APP/PS1 transgenic mouse. Although further analysis would be needed to confirm whether glibenclamide affects behavioral performance, our data suggests that glibenclamide may be a therapeutic option for AD treatment. | Ju, Yeon-Joo; Kim, Namkwon; Gee, Min Sung; Jeon, Seung Ho; Lee, Danbi; Do, Jimin; Ryu, Jong-Sik; Lee, Jong Kil | Kyung Hee Univ, Dept Fundamental Pharmaceut Sci, Grad Sch, 26 Kyungheedae Ro, Seoul 02447, South Korea; Kyung Hee Univ, Dept Life & Nanopharmaceut Sci, Grad Sch, 26 Kyungheedae Ro, Seoul 02447, South Korea; Kyung Hee Univ, Dept Biomed Sci & Technol, Grad Sch, 26 Kyungheedae Ro, Seoul 02447, South Korea; Kyungpook Natl Univ, Dept Phys Educ, Exercise Metab Lab, 80 Daehak Ro, Daegu, South Korea; Kyung Hee Univ, Coll Pharm, Dept Pharm, 26 Kyungheedae Ro, Seoul 02447, South Korea | Lee, Jong/AAK-8231-2020 | 57201153322; 56927286900; 57202802999; 57211874146; 57218343646; 57194011403; 57218342739; 25928433100 | jklee3984@khu.ac.kr; | EUROPEAN JOURNAL OF PHARMACOLOGY | EUR J PHARMACOL | 0014-2999 | 1879-0712 | 884 | SCIE | PHARMACOLOGY & PHARMACY | 2020 | 4.432 | 31.0 | 0.7 | 2025-06-25 | 15 | 14 | Alzheimer's disease; 5XFAD mice; BV2 microglial cells; Amyloid beta; Neuroinflammation; Glibenclamide | ALZHEIMERS-DISEASE; BV2 MICROGLIA; AMYLOID-BETA; NEUROINFLAMMATION; INFLAMMATION; BRAIN; MODEL | 5XFAD mice; Alzheimer's disease; Amyloid β; BV2 microglial cells; Glibenclamide; Neuroinflammation | Alzheimer Disease; Amyloid beta-Peptides; Amyloid beta-Protein Precursor; Animals; Anti-Inflammatory Agents; Behavior, Animal; Brain; Cell Line; Cytokines; Disease Models, Animal; Female; Glyburide; Humans; Inflammation Mediators; Male; Mice, Transgenic; Microglia; Mitogen-Activated Protein Kinases; Morris Water Maze Test; Mutation; NF-kappa B; Nitric Oxide; Presenilin-1; amyloid beta protein; cyclooxygenase 2; glibenclamide; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; interleukin 1beta; interleukin 6; lipopolysaccharide; messenger RNA; metformin; mitogen activated protein kinase 1; mitogen activated protein kinase 3; nitric oxide; stress activated protein kinase; transcription factor RelA; tumor necrosis factor; amyloid beta protein; amyloid precursor protein; antiinflammatory agent; APP protein, human; autacoid; cytokine; glibenclamide; immunoglobulin enhancer binding protein; mitogen activated protein kinase; nitric oxide; presenilin 1; PSEN1 protein, human; 5xFAD mouse; Alzheimer disease; animal cell; animal experiment; animal model; animal tissue; antiinflammatory activity; Article; BV-2 cell line; cell activation; cell function; cell stimulation; cell viability; controlled study; cytokine release; drug mechanism; female; genetic transfection; hippocampus; male; MAPK signaling; microglia; mouse; nervous system inflammation; neuromodulation; nonhuman; priority journal; protein expression; spatial learning; spatial memory; animal; animal behavior; brain; cell line; disease model; drug effect; genetics; human; metabolism; microglia; Morris water maze test; mutation; pathology; pathophysiology; transgenic mouse | English | 2020 | 2020-10-05 | 10.1016/j.ejphar.2020.173416 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | Article | Mechanical and clinical evaluation of the effect of microscrew on root proximity and cortical bone thickness | Background/Objectives: Primary stability is required for successful use of microscrew. This study investigated correlations among biomechanical, morphological, and clinical values in relationship to root contact and different placement locations. Materials/Methods: Thirty-three microscrews were placed between the molars (n = 18) or in the body of the mandible (n = 15) in three pigs. Insertion torque, Periotest, resonance frequency analysis (RFA), and static and dynamic stiffness were measured. Cone beam computed tomography was performed before and after the insertion of microscrews. Interproximal microscrews were divided into root contacted microscrews (n = 9) and non-root contact microscrews (n = 9). Factorial analysis of variance was conducted, with significance set at P < 0.05. Results: A significant difference was observed between bodily and root contacted microscrews in Periotest, RFA, static and dynamic stiffness, Tan delta, and bone density (RFA, P = 0.045; all others, P < 0.001). A significant difference was observed between bodily and non-root contact microscrews in Periotest, RFA, and bone density (RFA, P = 0.025; all others, P < 0.001). A significant difference was observed in static (P = 0.01) and dynamic (P = 0.038) stiffness between microscrews with and without contact. Dynamic stiffness (P = 0.02) and Tan delta (P = 0.03) showed significant correlations with Periotest results only in bodily microscrews. Limitations: Since a pig bone was used, some differences in the quality and quantity of the bone might be observed between humans. Conclusions/Implications: Stiffness values distinguished between microscrews with and without contact. Periotest and RFA results indicated that bodily microscrews were more stable than interproximal microscrews. Periotest and RFA may be useful with large, microscrews and/or in thick cortical bone, but further investigation is required to determine the stability of interproximal microscrews. | Tsatalis, Andrea E.; Watanabe, Keiichiro; Mitchell, Bobby; Kim, Do-Gyoon; Lee, Damian J.; Zheng, Fengyuan; Kyung, Hee-Moon; Deguchi, Toru | Ohio State Univ, Coll Dent, Columbus, OH 43210 USA; Ohio State Univ, Coll Dent, Div Orthodont, 4088 Postle Hall,305 W 12th Ave, Columbus, OH 43210 USA; Ohio State Univ, Columbus, OH 43210 USA; Ohio State Univ, Coll Dent, Div Restorat Sci & Prosthodont, Columbus, OH 43210 USA; Kyungpook Natl Univ, Sch Dent, Dept Orthodont, Daegu, South Korea | Kim, Dogyoon/D-5543-2012; Watanabe, Keiichiro/HGB-8792-2022; Kim, Hyongbum/D-5804-2019 | deguchi.4@osu.edu; | EUROPEAN JOURNAL OF ORTHODONTICS | EUR J ORTHODONT | 0141-5387 | 1460-2210 | 42 | 2 | SCIE | DENTISTRY, ORAL SURGERY & MEDICINE | 2020 | 3.075 | 31.0 | 6 | MINI-IMPLANT STABILITY; MINISCREW IMPLANTS; ORTHODONTIC ANCHORAGE; DAMPING CAPACITY; PLACEMENT TORQUE; INSERTION TORQUE; FAILURE RATE; MAXILLARY; CONTACT; VALUES | English | 2020 | 2020-04 | 10.1093/ejo/cjz017 | 바로가기 | 바로가기 | 바로가기 | ||||||||||
| ○ | ○ | Article | Positive allosteric modulation of GABAA receptors by a novel antiepileptic drug cenobamate | Cenobamate is a novel antiepileptic drug under investigation for use in patients with focal (partial-onset) seizures. To understand its potential molecular mechanism of action, the effects of cenobamate on GABA(A) -mediated currents and GABA(A) receptors in rodent hippocampal neurons were examined. Cenobamate potentiated GABA-induced currents (I-GABA) in acutely isolated CA3 pyramidal cells in a concentration-dependent manner (EC50, 164 mu M), which was not affected by flumazenil, a benzodiazepine receptor antagonist. Cenobamate enhanced tonic GABA(A) currents (I-tonic), which is defined as a holding current shift by the GABA(A) receptor antagonist bicuculline (EC50, 36.63 mu M). At therapeutically relevant concentrations, cenobamate induced minimal changes in the frequency, amplitudes, and decay time of spontaneous inhibitory postsynaptic currents in the CA1 neurons. Flumazenil failed to affect cenobamate-potentiated I-tonic and I-phasic in CA1 neurons. Cenobamate showed positive allosteric modulation of GABA-induced I-GABA mediated by GABA(A) receptors. This effect was similar for all tested hGABA(A) receptors containing six different alpha subunits (alpha(1)beta(2)gamma(2) or alpha(2-6)beta(3)gamma(2)), with EC50 values ranging from 42 to 194 mu M. Cenobamate did not displace the binding of flunitrazepam, a benzodiazepine derivative, or flumazenil to GABA(A) receptors. The results showed that cenobamate, a novel antiepileptic drug, acts as a positive allosteric modulator of high-affinity GABA(A) receptors, activated by GABA at a site independent of the benzodiazepine binding site and efficiently enhances I-tonic inhibition in hippocampal neurons, which could be an underlying molecular mechanism stabilizing neural circuits of the epileptic hippocampus. | Sharma, Ramesh; Nakamura, Michiko; Neupane, Chiranjivi; Jeon, Byeong Hwa; Shin, Hyewon; Melnick, Susan M.; Glenn, Kelli J.; Jang, Il-Sung; Park, Jin Bong | Chungnam Natl Univ, Coll Med, Dept Physiol, 6 Munhwa Ro, Daejeon 35015, South Korea; Chungnam Natl Univ, Brain Res Inst, 6 Munhwa Ro, Daejeon 35015, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Pharmacol, 2177 Dalgubeol Daero, Daegu 700412, South Korea; SK Biopharmaceut Co Ltd, 221 Pangyoyeok Ro, Seongnam 305712, Gyeonggi, South Korea; SK Life Sci Inc, 461 Rd,Fifth Floor, Paramus, NJ 07652 USA | ; Jeon, Byeong/D-4160-2012 | 57212267602; 36051866000; 57204142942; 35292492700; 55352187200; 57216543223; 40261596300; 7102177910; 57265760800 | ramesh.sharma.rs1991@gmail.com;michiko21a@hotmail.com;chiran.gbn913@gmail.com;bhjeon@cnu.ac.kr;hyewon.shin@sk.com;melnicks@sklsi.com;kglenn@sklsi.com;jis7619@knu.ac.kr;jinbong@cnu.ac.kr; | EUROPEAN JOURNAL OF PHARMACOLOGY | EUR J PHARMACOL | 0014-2999 | 1879-0712 | 879 | SCIE | PHARMACOLOGY & PHARMACY | 2020 | 4.432 | 31.0 | 5.1 | 2025-06-25 | 94 | 97 | GABA(A) receptors; Epilepsy; Antiepileptic drugs; Hippocampal neurons; GABA-Mediated currents | COOPERATIVE INTERACTIONS; GABAERGIC MECHANISMS; MUSCARINIC RECEPTORS; TONIC INHIBITION; BRUCINE ANALOGS; GRANULE CELLS; MODEL; ACETYLCHOLINE; TRANSMISSION; ACTIVATION | Antiepileptic drugs; Epilepsy; GABA-Mediated currents; GABA<sub>A</sub> receptors; Hippocampal neurons | Animals; Anticonvulsants; CA1 Region, Hippocampal; CA3 Region, Hippocampal; Carbamates; Chlorophenols; Female; HEK293 Cells; Humans; Male; Neurons; Rats, Sprague-Dawley; Rats, Wistar; Receptors, GABA-A; Tetrazoles; 4 aminobutyric acid A receptor; 4 aminobutyric acid A receptor alpha1; 4 aminobutyric acid A receptor alpha2; anticonvulsive agent; benzodiazepine; bicuculline; cenobamate; flumazenil; flunitrazepam; 4 aminobutyric acid A receptor; carbamic acid derivative; cenobamate; chlorophenol; tetrazole derivative; action potential amplitude; allosterism; animal cell; animal experiment; animal tissue; Article; brain electrophysiology; cell isolation; concentration response; controlled study; drug binding site; drug effect; drug mechanism; drug receptor binding; EC50; female; GABAergic system; hippocampal CA3 region; inhibitory postsynaptic potential; male; neuromodulation; nonhuman; priority journal; protein function; pyramidal nerve cell; rat; receptor affinity; rodent; animal; HEK293 cell line; hippocampal CA1 region; human; nerve cell; physiology; Sprague Dawley rat; Wistar rat | English | 2020 | 2020-07-15 | 10.1016/j.ejphar.2020.173117 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | Article | Prediction of Dowel Action against Concrete Core without Consideration of Transverse Reinforcement | In this study, reinforcing bars acting as dowels were investigated. Direct shear tests on specimens with a wide range of strengths for concrete (30 and 60 MPa) and steel (400 and 600 MPa) were conducted. Furthermore, finite-element (FE) models with selected concrete and steel material models, such as damage plasticity and ductile damage models, were developed. The existing predictive equations tended to show either larger or smaller maximum dowel forces depending on the size of the dowel. Such results were obtained because consideration of the effect of dowel action in design equations for interface shear transfer were originally based on the bending resistance of the reinforcement rather than splitting failure of the concrete body. This is justifiable, because in many practical situations splitting failure is prevented by the presence of confining reinforcement or by sufficient edge distance. The different results between the predictive equations and test data for maximum dowel force were minimized by calibrating the weight factor of the dowel bars. Accordingly, new equations are proposed to improve the accuracy of maximum dowel force and the corresponding slip; the new equations use data from the tests, analytical results for the current model, and from the literature. To estimate the maximum dowel forces, different coefficients were proposed depending on the failure mode and yield strength of the dowel bar. The average absolute difference ratios for the maximum dowel force and slip from a 400-MPa dowel bar were improved from 29% to 13% and 49% to 21%, respectively. The newly developed model successfully predicted the overall behavior of dowel action in a concrete structure without transverse reinforcement. DOI: 10.1061/(ASCE)ST.1943-541X.0002727. (c) 2020 American Society of Civil Engineers. | Jeong, Euiseok; Lee, Kyoung-Chan; Lee, Seong-Cheol; Seo, Junwon; Lee, Jaeha | South Dakota State Univ, Dept Civil & Environm Engn, Box 2219 Univ Stn, Brookings, SD 57007 USA; Korea Railrd Res Inst, Adv Railrd Civil Engn Div, 176 Cheoldobangmulgwan Ro, Uiwang Si 16105, Gyeonggi Do, South Korea; Kyungpook Natl Univ, Dept Civil Engn, 80 Daehakro, Daegu 41566, South Korea; Natl Korea Maritime & Ocean Univ, Dept Civil Engn, 727 Taejong Ro, Busan 49112, South Korea | lee, kyoung/D-7303-2013; Lee, Jaeha/AAD-3173-2022; Seo, Junwon/AAH-8029-2019 | leonlee397@gmail.com; | JOURNAL OF STRUCTURAL ENGINEERING | J STRUCT ENG | 0733-9445 | 1943-541X | 146 | 12 | SCIE | CONSTRUCTION & BUILDING TECHNOLOGY;ENGINEERING, CIVIL | 2020 | 3.312 | 31.0 | 10 | DAMAGE MODEL; BEHAVIOR; BARS | English | 2020 | 2020-12 | 10.1061/(asce)st.1943-541x.0002727 | 바로가기 | 바로가기 | 바로가기 | ||||||||||
| ○ | Article | High-density linkage map reveals QTL for Type-I seed coat cracking in RIL population of soybean [Glycine max (L.) Merr.] | Seed coat cracking (SCC), particularly the Type-I irregular cracking, is critical in determining the quality of appearance and commercial value of soybean seeds. The objective of this study was to identify the quantitative trait loci (QTLs) for SCC with high-density genetic map. One hundred sixty-seven recombinant inbred lines (RILs) developed from a cross between Uram (SCC-resistant) and Chamol (SCC-susceptible) were evaluated for SCC over 2 years (2016–2017). The QTL analysis identified 12 QTLs located on chromosomes 2 (D1b), 6 (C2), 8 (A2), 9 (K), 10 (O), 12 (H), 19 (L), and 20 (I). Out of the 12 QTLs, qSC2-1, qSC9, SC10-1, qSC10-2, and qSC12 were novel QTLs and the other seven QTLs (qSC2-2, qSC2-3, qSC6, qSC8, qSC19-1, qSC19-2, and qSC20) were found to co-localize with the previously identified QTLs. The mean SCC of the RILs of early maturity group was significantly higher than that of the late maturity group, suggesting an association between SCC and maturity loci. In addition, although 10 QTLs were distantly located from the maturity loci (E1, E3, E4, E7, and E10), qSC10-1 and qSC10-2 co-localized with the maturity loci E2. The results obtained in this study provide useful genetic information on SCC which could be used in the SCC breeding programs. © 2020, The Author(s). | Kang, Beom Kyu; Seo, Jeong Hyun; Jo, Hyun; Kulkarni, Krishnanand P.; Choi, Man Soo; Kim, Hyun Tae; Lee, Jeong Dong; Dhungana, Sanjeev K.; Kim, Hong Sik; Oh, Jae Hyeon; Park, Ji Hee; Shin, Sang Ouk; Baek, In Youl | Upland Crop Breeding Research Division, Department of Southern Area Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA), Miryang, 50424, South Korea; Upland Crop Breeding Research Division, Department of Southern Area Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA), Miryang, 50424, South Korea; School of Applied Biosciences, Kyungpook National University (KNU), Daegu, 41566, South Korea; Department of Agriculture and Natural Resources, College of Agriculture, Science and Technology, Delaware State University, Dover, 19901, DE, United States; Crop Foundation Research Division, National Institute of Crop Science (NICS), Rural Development Administration (RDA), Jeonju, 55365, South Korea; Upland Crop Breeding Research Division, Department of Southern Area Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA), Miryang, 50424, South Korea; School of Applied Biosciences, Kyungpook National University (KNU), Daegu, 41566, South Korea; Upland Crop Breeding Research Division, Department of Southern Area Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA), Miryang, 50424, South Korea; Upland Crop Breeding Research Division, Department of Southern Area Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA), Miryang, 50424, South Korea; Gene Engineering Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences (NAAS), Rural Development Administration (RDA), Jeonju, 54874, South Korea; Upland Crop Breeding Research Division, Department of Southern Area Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA), Miryang, 50424, South Korea; Upland Crop Breeding Research Division, Department of Southern Area Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA), Miryang, 50424, South Korea; Upland Crop Breeding Research Division, Department of Southern Area Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA), Miryang, 50424, South Korea | 56581053300; 57211702766; 57194539358; 8241514400; 57220545872; 57214326544; 40462075100; 56269940800; 56981510700; 57109658100; 57195458135; 24391353700; 24390414900 | hellobk01@korea.kr; | Euphytica | EUPHYTICA | 0014-2336 | 1573-5060 | 216 | 10 | SCIE | AGRONOMY;HORTICULTURE;PLANT SCIENCES | 2020 | 1.895 | 31.1 | 0.3 | 2025-06-25 | 3 | Maturity; QTL; Seed appearance; Seed coat cracking; Soybean; Type-I irregular cracking | English | Final | 2020 | 10.1007/s10681-020-02684-w | 바로가기 | 바로가기 | 바로가기 | ||||||||
| ○ | ○ | Article | Tubulosine selectively inhibits JAK3 signalling by binding to the ATP-binding site of the kinase of JAK3 | Gain- or loss-of-function mutations in Janus kinase 3 (JAK3) contribute to the pathogenesis of various haematopoietic malignancies and immune disorders, suggesting that aberrant JAK3 signalling is an attractive therapeutic target to treat these disorders. In this study, we performed structure-based computational database screening using the 3D structure of the JAK3 kinase domain and the National Cancer Institute diversity set and identified tubulosine as a novel JAK3 inhibitor. Tubulosine directly blocked the catalytic activity of JAK3 by selective interacting with the JAK3 kinase domain. Consistently, tubulosine potently inhibited persistently activated and interleukin-2-dependent JAK3, and JAK3-mediated downstream targets. Importantly, it did not affect the activity of other JAK family members, particularly prolactin-induced JAK2/signal transducer and activator of transcription 5 and interferon alpha-induced JAK1-TYK2/STAT1. Tubulosine specifically decreased survival and proliferation of cancer cells, in which persistently active JAK3 is expressed, by inducing apoptotic and necrotic/autophagic cell death without affecting other oncogenic signalling. Collectively, tubulosine is a potential small-molecule compound that selectively inhibits JAK3 activity, suggesting that it may serve as a promising therapeutic candidate for treating disorders caused by aberrant activation of JAK3 signalling. | Kim, Byung-Hak; Yi, Eun Hee; Jee, Jun-Goo; Jeong, Ae Jin; Sandoval, Claudio; Park, In-Chul; Baeg, Gyeong Hun; Ye, Sang-Kyu | New York Med Coll, Dept Pediat, Valhalla, NY 10595 USA; Seoul Natl Univ, Dept Pharmacol, Coll Med, Seoul 03080, South Korea; Seoul Natl Univ, Biomed Sci Project BK21PLUS, Coll Med, Seoul, South Korea; Seoul Natl Univ, Ischem Hypox Dis Inst, Coll Med, Seoul, South Korea; Kyungpook Natl Univ, Res Inst Pharmaceut Res, Coll Pharm, Daegu, South Korea; Korea Inst Radiol & Med Sci, Div Basic Radiat Biosci, Seoul, South Korea; Chinese Univ Hong Kong, Sch Life & Hlth Sci, 2001 Longxiang Blvd, Shenzhen, Peoples R China; Seoul Natl Univ, Neuroimmune Informat Storage Network Res Ctr, Coll Med, Seoul, South Korea | Kim, Byung-Hak/AAY-9891-2020 | 36068548000; 57211316954; 7004327823; 57206532004; 7005249609; 59283010900; 6603387513; 8956669200 | ghbaeg@cuhk.edu.cn;sangkyu@snu.ac.kr; | JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | J CELL MOL MED | 1582-1838 | 1582-4934 | 24 | 13 | SCIE | CELL BIOLOGY;MEDICINE, RESEARCH & EXPERIMENTAL | 2020 | 5.31 | 31.1 | 0.38 | 2025-06-25 | 9 | 10 | JAK3; leukaemia; lymphoma; STAT; structure-based computational database screening; tubulosine | STRUCTURAL BASIS; TYROSINE KINASE; JANUS; ACTIVATION; MUTATIONS; IDENTIFICATION; EXPRESSION; COMPLEX; INTERLEUKIN-2; DEFICIENCY | JAK3; leukaemia; lymphoma; STAT; structure-based computational database screening; tubulosine | Adenosine Triphosphate; Apoptosis; Autophagy; Binding Sites; Cell Line, Tumor; Cell Proliferation; Cell Survival; Emetine; Humans; Janus Kinase 3; Models, Biological; Necrosis; Oncogenes; Signal Transduction; STAT5 Transcription Factor; adenosine triphosphate; caspase 3; cyclin dependent kinase 2; interleukin 15; interleukin 23; interleukin 7; mitogen activated protein kinase 1; mitogen activated protein kinase 3; mitogen activated protein kinase p38; nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase; nilotinib; protein Bax; protein bcl 2; small interfering RNA; STAT3 protein; STAT5 protein; stress activated protein kinase; survivin; adenosine triphosphate; emetine; Janus kinase 3; STAT5 protein; tubulosine; apoptosis; Article; binding site; bioinformatics; cell proliferation; cell survival; cell viability; cell viability assay; cytotoxicity; EC50; flow cytometry; fluorescence activated cell sorting; gain of function mutation; gene therapy; hematopoietic stem cell transplantation; IC50; immunoblotting; immunoprecipitation; JAK-STAT signaling; loss of function mutation; metatarsophalangeal joint; molecular docking; MTT assay; priority journal; protein binding; protein expression; protein phosphorylation; TUNEL assay; upregulation; Western blotting; autophagy; binding site; biological model; chemistry; drug effect; human; metabolism; necrosis; oncogene; signal transduction; tumor cell line | English | 2020 | 2020-07 | 10.1111/jcmm.15362 | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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