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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | ○ | Article | Development of a photochemical thrombosis investigation system to obtain a rabbit ischemic stroke model | Photochemical thrombosis is a method for the induction of ischemic stroke in the cerebral cortex. It can generate localized ischemic infarcts in the desired region; therefore, it has been actively employed in establishing an ischemic stroke animal model and in vivo assays of diagnostic and therapeutic techniques for stroke. To establish a rabbit ischemic stroke model and overcome the shortcoming of previous studies that were difficult to build a standardized photothrombotic rabbit model, we developed a photochemical thrombosis induction system that can produce consistent brain damage on a specific area. To verify the generation of photothrombotic brain damage using the system, longitudinal magnetic resonance imaging, 2,3,5-triphenyltetrazolium chloride staining, and histological staining were applied. These analytical methods have a high correlation for ischemic infarction and are appropriate for analyzing photothrombotic brain damage in the rabbit brain. The results indicated that the photothrombosis induction system has a main advantage of being accurately controlled a targeted region of photothrombosis and can produce cerebral hemisphere lesions on the target region of the rabbit brain. In conjugation with brain atlas, it can induce photochemical ischemic stroke locally in the part of the brain that is responsible for a particular brain function and the system can be used to develop animal models with degraded specific functions. Also, the photochemical thrombosis induction system and a standardized rabbit ischemic stroke model that uses this system have the potential to be used for verifications of biomedical techniques for ischemic stroke at a preclinical stage in parallel with further performance improvements. | Kim, Yoonhee; Lee, Yoon Bum; Bae, Seung Kuk; Oh, Sung Suk; Choi, Jong-ryul | Daegu Gyeongbuk Med Innovat Fdn DGMIF, Med Device Dev Ctr, Daegu 41061, South Korea; Daegu Gyeongbuk Med Innovat Fdn DGMIF, Lab Anim Ctr, Daegu 41061, South Korea; Kyungpook Natl Univ, Dept Biofibers & Biomat Sci, Daegu 41566, South Korea | 57222362589; 57222355818; 57210116959; 57193393986; 24773058200 | ssoh@dgmif.re.kr;jongryul32@dgmif.re.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 11 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2021 | 4.997 | 25.0 | 0.59 | 2025-07-30 | 14 | 14 | DIRECT-CURRENT STIMULATION; CEREBRAL-ISCHEMIA; PHOTOTHROMBOTIC STROKE; INTRAVENOUS TPA; BRAIN-DAMAGE; FLUORO-JADE; RAT; THERAPY; MOUSE; MRI | Animals; Brain; Disease Models, Animal; Ischemic Stroke; Magnetic Resonance Imaging; Male; Photochemical Processes; Rabbits; Thrombosis; animal; brain; brain ischemia; diagnostic imaging; disease model; Leporidae; male; nuclear magnetic resonance imaging; pathology; photochemistry; thrombosis | English | 2021 | 2021-03-11 | 10.1038/s41598-021-85348-6 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||||
| ○ | ○ | Article | Development? of fully automated anterior chamber cell analysis based on image software | Optical coherence tomography (OCT) is a noninvasive method that can quickly and accurately examine the eye at the cellular level. Several studies have used OCT for analysis of anterior chamber cells. However, these studies have several limitations. This study was performed to supplement existing reports of automated analysis of anterior chamber cell images using spectral domain OCT (SD-OCT) and to compare this method with the Standardization of Uveitis Nomenclature (SUN) grading system. We analyzed 2398 anterior segment SD-OCT images from 34 patients using code written in Python. Cell density, size, and eccentricity were measured automatically. Increases in SUN grade were associated with significant cell density increases at all stages (p<0.001). Significant differences were observed in eccentricity in uveitis, post-surgical inflammation, and vitreous hemorrhage (p<0.001). Anterior segment SD-OCT is reliable, fast, and accurate means of anterior chamber cell analysis. This method showed a strong correlation with the SUN grade system. Also, eccentricity could be helpful as a supplementary evaluation tool. | Kang, Tae Seen; Lee, Yeongseop; Lee, Seongjin; Kim, Kyonghoon; Lee, Woong-sub; Lee, Woohyuk; Kim, Jin Hyun; Han, Yong Seop | Gyeongsang Natl Univ, Dept Ophthalmol, Changwon Hosp, 11 Samjeongja Ro, Chang Won 51472, South Korea; Gyeongsang Natl Univ, Dept Convergence Engn, Jinju, South Korea; Kyungpook Natl Univ, Sch Comp Sci & Engn, Daegu, South Korea; Gyeongsang Natl Univ, Dept Informat & Commun Engn, Tongyeong, South Korea; Gyeongsang Natl Univ, Dept Ophthalmol, Coll Med, Jinju, South Korea; Gyeongsang Natl Univ, Hlth Sci Inst, Jinju, South Korea | Lee, Seongjin/AAI-2365-2020 | 57020662400; 57219242110; 24824950900; 15065463500; 57223965814; 57193846826; 56242902800; 55489221800 | medcabin@naver.com; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 11 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2021 | 4.997 | 25.0 | 0.38 | 2025-07-30 | 8 | 8 | OPTICAL COHERENCE TOMOGRAPHY; INTEROBSERVER AGREEMENT; UVEITIS; INFLAMMATION | Aged; Algorithms; Anterior Chamber; Cell Count; Cell Tracking; Female; Humans; Image Processing, Computer-Assisted; Male; Middle Aged; Software; Tomography, Optical Coherence; aged; algorithm; anterior eye chamber; cell count; cell tracking; cytology; female; human; image processing; male; middle aged; optical coherence tomography; procedures; software | English | 2021 | 2021-05-21 | 10.1038/s41598-021-89794-0 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Effect of a bioconverted product of Lotus corniculatus seed on the axillary microbiome and body odor | The skin microbiome, especially the axillary microbiome, consists of odor-causing bacteria that decompose odorless sweat into malodor compounds, which contributes to the formation of body odor. Plant-derived products are a cheap source of bioactive compounds that are common ingredients in cosmetics. Microbial bioconversion of natural products is an ecofriendly and economical method for production of new or improved biologically active compounds. Therefore, in this study, we tested the potential of a Lactobacillus acidophilus KNU-02-mediated bioconverted product (BLC) of Lotus corniculatus seed to reduce axillary malodor and its effect on the associated axillary microbiota. A chemical profile analysis revealed that benzoic acid was the most abundant chemical compound in BLC, which increased following bioconversion. Moreover, BLC treatment was found to reduce the intensity of axillary malodor. We tested the axillary microbiome of 18 study participants, divided equally into BLC and placebo groups, and revealed through 16S rRNA gene sequencing that Staphylococcus, Corynebacterium, and Anaerococcus were the dominant taxa, and some of these taxa were significantly associated with axillary malodor. After one week of BLC treatment, the abundance of Corynebacterium and Anaerococcus, which are associated with well-known odor-related genes that produce volatile fatty acids, had significantly reduced. Likewise, the identified odor-related genes decreased after the application of BLC. BLC treatment enhanced the richness and network density of the axillary microbial community. The placebo group, on the other hand, showed no difference in the microbial richness, odor associated taxa, and predicted functional genes after a week. The results demonstrated that BLC has the potential to reduce the axillary malodor and the associated odorcausing bacteria, which makes BLC a viable deodorant material in cosmetic products. | Kim, Min-Ji; Tagele, Setu Bazie; Jo, HyungWoo; Kim, Min-Chul; Jung, YeonGyun; Park, Yeong-Jun; So, Jai-Hyun; Kim, Hae Jin; Kim, Ho Jin; Lee, Dong-Geol; Kang, Seunghyun; Shin, Jae-Ho | Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea; COSMAX BTI, R&I Ctr, Seongnam 13486, South Korea; Natl Dev Inst Korean Med, 94 Hwarang Ro, Gyongsan 38540, Gyeongsangbuk D, South Korea; Natl Agr Prod Qual Management Serv, Expt Res Inst, Gimcheon 39660, Gyeongsangbuk D, South Korea | Tagele, Setu Bazie/IYJ-1959-2023; Lee, Dong-geol/AAI-2838-2021; shin, Jaeho/K-6792-2013 | 57127351600; 57202007103; 57197827666; 57203466596; 57197833801; 56275800900; 36185972100; 59052092300; 56372903900; 55559897800; 57188724232; 57224125922 | jhshin@knu.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 11 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2021 | 4.997 | 25.0 | 0.32 | 2025-07-30 | 3 | 7 | DEODORANTS; ALUMINUM | Axilla; Female; Humans; Lotus; Metagenomics; Microbiota; Odorants; Phytochemicals; Plant Extracts; Seeds; Skin; fragrance; phytochemical; plant extract; axilla; chemistry; drug effect; female; human; Lotus (genus); metagenomics; microbiology; microflora; plant seed; procedures; skin | English | 2021 | 2021-05-12 | 10.1038/s41598-021-89606-5 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Effect of electrodes positions on the performance of microfluidic enzymatic biofuel cell: From two streams to a single-stream flow device | Membraneless microfluidic enzymatic biofuel cells (mu EBFCs) operated under the co-laminar flow of fuel and oxidant often face cross-mixing issues, especially at low flow rates. In this work, we propose a new approach to design the single-stream mu EBFC based on the investigation of relative positions of the electrodes at the top and bottom of the microchannel. To achieve this, a mu EBFC was fabricated with multiwalled carbon nanotube (MWCNT) electrodes via a stencil method. The bioanode and biocathode were modified with glucose dehydrogenase and laccase via direct covalent bonding, respectively. The best results were attained by placing the cathode at the top and anode at the bottom of the microchannel in a Y-shaped two streamflow mu EBFC. When a single electrolyte stream was used to achieve more practicality and ease of use, the performance was reduced by 40%. However, with the new design of electrodes in single-stream mu EBFC, i.e. cathode at the top and anode at the bottom facing each other in the microchannel, the performance was recovered by 20%. This corresponds to maximum current and power density of 216 +/- 12 mu A cm(-2) and 69.2 +/- 9.2 mu W cm(-2), respectively. The stacking ability of the device was realized by connecting the two cells in series and parallel. The maximum power density delivered by the two single-stream mu EBFCs' stack reached 160 mu W cm(-2) at 0.3 V. Thus, this study validates the viability of using single-stream mu EBFCs in large stacks to power microelectronics more simply and practically. (C) 2021 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved. | Khan, Haroon; Ullah, Asad; Shin, Min Chul; Kwak, Moon Kyu; Kim, Gyu Man | Kyungpook Natl Univ, Sch Mech Engn, Daegu 41566, South Korea | Kwak, Moon/AGQ-6058-2022; Shin, Min/AAU-6206-2021 | 14521310700; 57226132377; 57226714930; 57203947984; 55664733000 | gyuman.kim@knu.ac.kr; | INTERNATIONAL JOURNAL OF HYDROGEN ENERGY | INT J HYDROGEN ENERG | 0360-3199 | 1879-3487 | 46 | 67 | SCIE | CHEMISTRY, PHYSICAL;ELECTROCHEMISTRY;ENERGY & FUELS | 2021 | 7.139 | 25.0 | 0.13 | 2025-07-30 | 5 | 5 | Microfluidic; Enzymatic biofuel cell; Stencil; Electrode positions; Single-stream | IMPROVED FUEL UTILIZATION; POWER-GENERATION; GLUCOSE; DESIGN | Electrode positions; Enzymatic biofuel cell; Microfluidic; Single-stream; Stencil | Anodes; Biofuels; Cathodes; Electrolytes; Laminar flow; Microelectronics; Microfluidics; Multiwalled carbon nanotubes (MWCN); Electro-deposition; Flow devices; Low flow; Maximum power density; Membraneless; New approaches; Performance; Single-stream; Stencil; Two-stream; Microchannels | English | 2021 | 2021-09-28 | 10.1016/j.ijhydene.2021.07.153 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |
| ○ | ○ | Article | Effect of intraoperative fluid volume on postoperative ileus after robot-assisted radical cystectomy | This study aimed to investigate the effect of intraoperative fluid volume on the postoperative ileus (POI) recovery period. A retrospective review of the Korean robot-assisted radical cystectomy database identified 718 patients who underwent robot-assisted radical cystectomy (RARC). Regression analyses were performed to identify the associations between the amount of intraoperative fluid administration (crystalloid/colloid/total), POI period (time to flatus/bowel movements), and length of hospital stay (LOS) after adjusting for covariates. In addition, we analyzed the risk factors for gastrointestinal complications and prolonged POI using a logistic regression model. An increasing volume of the administered crystalloid/total fluid was associated with prolonged POI (crystalloid R-2=0.0725 and P<0.0001; total amount R-2=0.0812 and P<0.0001), and the total fluid volume was positively associated with the LOS (R-2=0.099 and P<0.0001). The crystalloid amount was a risk factor for prolonged POI (P<0.001; odds ratio, 1.361; 95% confidence interval, 1.133-1.641; P<0.001). In the context of RARC, increased intravenous fluids are associated with prolonged POI and longer LOS. | Shim, Ji Sung; Noh, Tae Il; Ku, Ja Hyeon; Lee, Sangchul; Kwon, Tae Gyun; Kim, Tae-Hwan; Jeon, Seung Hyun; Lee, Sang Hyup; Nam, Jong Kil; Kim, Wan Seok; Jeong, Byong Chang; Lee, Ji Youl; Hong, Sung Hoo; Rha, Koon Ho; Han, Woong Kyu; Ham, Won Sik; Lee, Young Goo; Lee, Yong Seong; Park, Sung Yul; Yoon, Young Eun; Kang, Sung Gu; Oh, Jong Jin; Kang, Seok Ho | Korea Univ, Dept Urol, Coll Med, 73 Inchon Ro, Seoul 02841, South Korea; Seoul Natl Univ, Dept Urol, Coll Med, Seoul, South Korea; Kyungpook Natl Univ, Dept Urol, Sch Med, Daegu, South Korea; Kyung Hee Univ, Dept Urol, Sch Med, Seoul, South Korea; Pusan Natl Univ, Dept Urol, Yangsan Hosp, Yangsan, South Korea; Inje Univ, Busan Paik Hosp, Coll Med, Dept Urol, Busan, South Korea; Sungkyunkwan Univ, Samsung Med Ctr, Dept Urol, Sch Med, Seoul, South Korea; Catholic Univ Korea, Coll Med, Dept Urol, Seoul St Marys Hosp, Seoul, South Korea; Yonsei Univ, Severance Hosp, Dept Urol, Coll Med, Seoul, South Korea; Hallym Univ, Sch Med, Dept Urol, Kangnam Sacred Heart Hosp, Seoul, South Korea; Hallym Univ, Coll Med, Dept Urol, Sacred Heart Hosp, Anyang, South Korea; Hanyang Univ, Dept Urol, Coll Med, Seoul, South Korea; Seoul Natl Univ, Dept Urol, Bundang Hosp, Seongnam, South Korea | Lee, Hye/J-2154-2015; Jeon, Seung Hyun/AFN-3212-2022; Yoon, Youngeun/HOC-1137-2023; LEE, Sunghee/HCH-4510-2022; Lee, Jung-Seok/L-6826-2019; Kim, Tae-Hwan/M-3962-2017; Shim, Ji/AAY-3169-2020; Ku, Ja/E-6682-2016 | 55145514900; 55181695500; 36800523000; 57202327303; 59068063800; 57797823600; 7203005251; 57192520266; 8653467500; 57217264796; 7102237943; 57202074202; 37030299600; 7003388459; 7401899857; 23097952400; 57226514048; 57226509630; 12242293700; 57226509445; 57216641692; 24468588100; 7405684686; 15073765400; 57207888185; 55965389800; 59528193600; 35995660400 | urojin@snu.ac.kr;mdksh@korea.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 11 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2021 | 4.997 | 25.0 | 0.43 | 2025-07-30 | 10 | 10 | COLORECTAL SURGERY; ENHANCED RECOVERY; BLADDER-CANCER; MANAGEMENT; MECHANISMS; RESTRICTION; MORBIDITY; RESECTION; OUTCOMES; BURDEN | Aged; Body Fluids; Cystectomy; Female; Humans; Ileus; Intraoperative Period; Length of Stay; Male; Middle Aged; Postoperative Complications; Retrospective Studies; Robotic Surgical Procedures; Treatment Outcome; adverse event; aged; body fluid; cystectomy; female; human; ileus; intraoperative period; length of stay; male; middle aged; postoperative complication; retrospective study; robot assisted surgery; treatment outcome | English | 2021 | 2021-05-18 | 10.1038/s41598-021-89806-z | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Effectiveness of liraglutide 3 mg for the treatment of obesity in a real-world setting without intensive lifestyle intervention | Objective We investigated the efficacy and safety of liraglutide 3 mg daily in combination with diet and exercise 2, 4, and 6 months after initiation in real-world settings in Korea. Methods People first using liraglutide starting in 2018 were recruited from ten sites in Korea. Body weight and body mass index (BMI) were measured after 2, 4, and 6 months and compared with baseline values. Results The full cohort comprised 769 participants: 672 in the 2-month group, 427 in the 4-month group, and 219 in the 6-month group. The baseline mean +/- standard deviation of BMI and body weight were 32.2 +/- 5.1 kg/m(2), and 87.5 +/- 18.8 kg, respectively. Body weight and BMI decreased after initiation of liraglutide treatment: -2.94 kg and -1.08 kg/m(2) at 2 months; -4.23 kg and -1.55 kg/m(2) at 4 months, and -5.14 kg and -1.89 kg/m(2) at 6 months (all P = 5% and >= 10% of body weight, respectively. After 6 months, systolic and diastolic blood pressure decreased significantly by 3.90 and 1.93 mmHg, respectively. In those with diabetes mellitus, HbA1c and fasting glucose levels decreased significantly by 1.14% and 27.8 mg/dl, respectively. Among all participants, 27.6% experienced adverse effects, including nausea (20.8%), vomiting (5.2%), diarrhoea (2.5%), and skin rash (3.6%). Documented reasons for discontinuation of treatment were lack of effect (4.4%), adverse events (4.3%), and high cost (3.1%). Conclusions In real-world settings in Korea, daily treatment with liraglutide 3 mg was associated with clinically meaningful weight loss without serious adverse events. | Park, Jung Ha; Kim, Ju Young; Choi, Jong Han; Park, Hye Soon; Shin, Hyun-Young; Lee, Jae Min; Kim, Jin-Wook; Ko, Hae-Jin; Chon, Suk; Kim, Bu Kyung; Kim, Chul Sik; Lim, Soo | Seoul Natl Univ, Dept Family Med, Bundang Hosp, Seongnam, South Korea; Jeju Natl Univ Hosp, Dept Family Med, Jeju, South Korea; Univ Ulsan, Dept Internal Med, Asan Med Ctr, Coll Med, Seoul, South Korea; Konkuk Univ, Med Ctr, Dept Internal Med, Sch Med, Seoul, South Korea; Univ Ulsan, Dept Family Med, Asan Med Ctr, Coll Med, Seoul, South Korea; Hanyang Univ, Myongji Hosp, Dept Family Med, Coll Med, Goyang, South Korea; Daejeon Endo Internal Med Clin, Daejeon, South Korea; Seongnam Cent Hosp, Dept Family Med, Obes & Metab Dis Ctr, Seongnam, South Korea; CHA Univ, Dept Family Med, CHAUM Med Check Up Ctr, CHA Bundang Med Ctr, Seongnam, South Korea; Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Dept Family Med, Daegu, South Korea; Kyung Hee Univ, Kyung Hee Univ Hosp, Dept Endocrinol & Metab, Coll Med, Seoul, South Korea; Kosin Univ, Dept Internal Med, Coll Med, Busan, South Korea; Yonsei Univ, Coll Med, Dept Internal Med, Yongin Severance Hosp, Yongin, South Korea; Seoul Natl Univ, Coll Med, Dept Internal Med, Bundang Hosp, Seongnam, South Korea | ; Kim, Jin/AAW-3716-2021; Kim, Juhee/KFS-3069-2024; Chon, Suk/AAV-3102-2020; Lim, Soo/AAU-8107-2020 | 57219448110; 57222879431; 55750862800; 57192958653; 13006389700; 57221598918; 57201935788; 54393415700; 8339069800; 54936025200; 15842030200; 26660890200 | limsoo@snu.ac.kr; | INTERNATIONAL JOURNAL OF OBESITY | INT J OBESITY | 0307-0565 | 1476-5497 | 45 | 4 | SCIE | ENDOCRINOLOGY & METABOLISM;NUTRITION & DIETETICS | 2021 | 5.551 | 25.0 | 2.32 | 2025-07-30 | 32 | 31 | GLUCAGON-LIKE PEPTIDE-1; WEIGHT-LOSS; CARDIOVASCULAR EVENTS; RECEPTOR AGONISTS; CONTROLLED-TRIAL; CELL FUNCTION; GLP-1 ANALOG; MANAGEMENT; ATTRITION; PREDICTORS | Adult; Blood Pressure; Body Mass Index; Body Weight; Exercise; Female; Heart Disease Risk Factors; Humans; Life Style; Liraglutide; Male; Middle Aged; Obesity; Republic of Korea; Weight Loss; dipeptidyl peptidase IV inhibitor; glucose; hemoglobin A1c; insulin; liraglutide; metformin; sodium glucose cotransporter 2 inhibitor; sulfonylurea; liraglutide; adult; Article; blood pressure regulation; body mass; body weight; body weight loss; cardiometabolic risk; cohort analysis; constipation; controlled study; diabetes mellitus; diarrhea; diastolic blood pressure; diet therapy; dizziness; drug cost; drug efficacy; drug safety; drug withdrawal; exercise; female; gastrointestinal symptom; glucose blood level; hemoglobin blood level; human; hypoglycemia; insulin treatment; lifestyle modification; major clinical study; male; nausea; obesity; observational study; priority journal; rash; retrospective study; South Korea; systolic blood pressure; treatment duration; vomiting; waist circumference; weakness; blood pressure; body weight loss; clinical trial; exercise; lifestyle; middle aged; multicenter study; obesity | English | 2021 | 2021-04 | 10.1038/s41366-021-00739-z | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Effects of silicon doping on low-friction and high-hardness diamond-like carbon coating via filtered cathodic vacuum arc deposition | In this study, silicon (Si) was doped on a tetrahedral amorphous carbon (ta-C) coating and the tribological characteristics of the resulting Si-doped diamond-like carbon (DLC; a-C:Si:H) were investigated against a SUJ2 ball. The Si fraction in the coating was varied from 0 to similar to 20 at.% by increasing the trimethylsilane gas flow rate during filtered cathodic vacuum arc deposition. The coefficient of friction (CoF) showed no obvious change when the Si fraction was less than similar to 7 at.%. However, after Si doping, it significantly decreased when the Si fraction was greater than similar to 8 at.%. The running-in period also decreased to less than 1000 cycles after Si doping. The rapid formation of Si-rich debris and transfer layer led to the fabrication of a low-friction tribofilm, which was induced by the tribochemical reaction with moisture under ambient conditions. When the Si fraction was similar to 17 at.%, the lowest CoF of less than 0.05 was obtained. Further Si doping beyond the critical point led to the destruction of the film because of reduced hardness. | Kim, Jae-Il; Jang, Young-Jun; Kim, Jisoo; Kim, Jongkuk | Korea Inst Mat Sci KIMS, Dept Extreme Environm Coatings, Surface Technol Div, 797 Changwondae Ro, Changwon Si 51508, Gyeongnam Do, South Korea; Kyungpook Natl Univ, Dept Precis Mech Engn, 2559 Gyeongsang Daero, Sangju 37224, Gyeongsangbuk D, South Korea; Kyungpook Natl Univ, Dept Adv Sci & Technol Convergence, 2559 Gyeongsang Daero, Sangju 37224, Gyeongsangbuk D, South Korea | Kim, Jae-Il/ABB-9123-2020; KIM, JI/U-1902-2018 | 57211474980; 56517492000; 57192647685; 50061608100 | js.kim@knu.ac.kr;kjongk@kims.re.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 11 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2021 | 4.997 | 25.0 | 2.16 | 2025-07-30 | 47 | 46 | TRIBOLOGICAL PROPERTIES; THERMAL-STABILITY; FILMS; DLC; MICROSTRUCTURE; CORROSION; BEHAVIOR; IMPROVEMENT; SURFACE; STEEL | English | 2021 | 2021-02-11 | 10.1038/s41598-021-83158-4 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||||
| ○ | ○ | Article | Endothelial angiogenic activity and adipose angiogenesis is controlled by extracellular matrix protein TGFBI | Several studies have suggested that extracellular matrix (ECM) remodeling and the microenvironment are tightly associated with adipogenesis and adipose angiogenesis. In the present study, we demonstrated that transforming growth factor-beta induced (TGFBI) suppresses angiogenesis stimulated by adipocyte-conditioned medium (Ad-CM), both in vitro and in vivo. TGFBI knockout (KO) mice exhibited increased numbers of blood vessels in adipose tissue, and blood vessels from these mice showed enhanced infiltration into Matrigel containing Ad-CM. The treatment of Ad-CM-stimulated SVEC-10 endothelial cells with TGFBI protein reduced migration and tube-forming activity. TGFBI protein suppressed the activation of the Src and extracellular signaling-related kinase signaling pathways of these SVEC-10 endothelial cells. Our findings indicated that TGFBI inhibited adipose angiogenesis by suppressing the activation of Src and ERK signaling pathways, possibly because of the stimulation of the angiogenic activity of endothelial cells. | Lee, Seul Gi; Kim, Jin Soo; Kim, Ha-Jeong; Schlaepfer, David D.; Kim, In-San; Nam, Ju-Ock | Kyungpook Natl Univ, Dept Food Sci & Biotechnol, Daegu 41566, South Korea; Natl Inst Korean Med Dev, Kyeongsan 38540, South Korea; Kyungpook Natl Univ, Sch Med, Dept Physiol, 680 Gukchaebosang Ro, Daegu 41944, South Korea; Univ Calif San Diego, Moores Canc Ctr, La Jolla, CA 92093 USA; Korea Univ, KU KIST Grad Sch Converging Sci & Technol, Seoul 02841, South Korea; Korea Inst Sci & Technol KIST, Biomed Res Inst, Seoul 02792, South Korea | 56995397800; 57194216639; 57191717512; 7004338110; 34770432800; 7201496105 | namjo@knu.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 11 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2021 | 4.997 | 25.0 | 0.92 | 2025-07-30 | 22 | 24 | TISSUE ANGIOGENESIS; REGIONAL DIFFERENCES; GENE-EXPRESSION; CELL-ADHESION; OBESITY; GROWTH; BETA-IG-H3; FIBROSIS; MICE | Adipose Tissue; Animals; Capillaries; Endothelium, Vascular; Extracellular Matrix Proteins; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Neovascularization, Physiologic; Transforming Growth Factor beta; betaIG-H3 protein; scleroprotein; transforming growth factor beta; adipose tissue; angiogenesis; animal; C57BL mouse; capillary; growth, development and aging; knockout mouse; male; metabolism; mouse; vascular endothelium; vascularization | English | 2021 | 2021-05-06 | 10.1038/s41598-021-88959-1 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||||
| ○ | ○ | Article | Estimating severe fever with thrombocytopenia syndrome transmission using machine learning methods in South Korea | Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne infectious disease in China, Japan, and Korea. This study aimed to estimate the monthly SFTS occurrence and the monthly number of SFTS cases in the geographical area in Korea using epidemiological data including demographic, geographic, and meteorological factors. Important features were chosen through univariate feature selection. Two models using machine learning methods were analyzed: the classification model in machine learning (CMML) and regression model in machine learning (RMML). We developed a novel model incorporating the CMML results into RMML, defined as modified-RMML. Feature importance was computed to assess the contribution of estimating the number of SFTS cases using modified-RMML. Aspect to the accuracy of the novel model, the performance of modified-RMML was improved by reducing the MSE for the test data as 12.6-52.2%, compared to the RMML using five machine learning methods. During the period of increasing the SFTS cases from May to October, the modified-RMML could give more accurate estimation. Computing the feature importance, it is clearly observed that climate factors such as average maximum temperature, precipitation as well as mountain visitors, and the estimation of SFTS occurrence obtained from CMML had high Gini importance. The novel model incorporating CMML and RMML models improves the accuracy of the estimation of SFTS cases. Using the model, climate factors, including temperature, relative humidity, and mountain visitors play important roles in transmitting SFTS in Korea. Our findings highlighted that the guidelines for mountain visitors to prevent SFTS transmissions should be addressed. Moreover, it provides important insights for establishing control interventions that predict early identification of SFTS cases. | Cho, Giphil; Lee, Seungheon; Lee, Hyojung | Pusan Natl Univ, Finance Fishery Manufacture Ind Math Ctr Big Data, Busan 46241, South Korea; Pusan Natl Univ, Dept Math, Busan 46241, South Korea; Kyungpook Natl Univ, Dept Stat, Daegu 41566, South Korea | 56665111500; 57325801500; 57196021198 | hjleebiomath@gmail.com; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 11 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2021 | 4.997 | 25.0 | 0.22 | 2025-07-30 | 7 | 7 | TICKS; SFTS | Aged; Climate; Computer Simulation; Epidemiological Models; Female; Humans; Machine Learning; Male; Middle Aged; Mountaineering; Regression Analysis; Republic of Korea; Risk Factors; Severe Fever with Thrombocytopenia Syndrome; Travel-Related Illness; aged; climate; computer simulation; female; human; machine learning; male; middle aged; mountaineering; regression analysis; risk factor; severe fever with thrombocytopenia syndrome; South Korea; travel related disease | English | 2021 | 2021-11-08 | 10.1038/s41598-021-01361-9 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||||
| ○ | ○ | Article | Evaluation of electroporated area using 2,3,5-triphenyltetrazolium chloride in a potato model | Irreversible electroporation (IRE) is a tissue ablation method, uses short high electric pulses and results in cell death in target tissue by irreversibly permeabilizing the cell membrane. Potato is commonly used as a tissue model for electroporation experiments. The blackened area that forms 12 h after electric pulsing is regarded as an IRE-ablated area caused by melanin accumulation. Here, the 2,3,5-triphenyltetrazolium chloride (TTC) was used as a dye to assess the IRE-ablated area 3 h after potato model ablation. Comparison between the blackened area and TTC-unstained white area in various voltage conditions showed that TTC staining well delineated the IRE-ablated area. Moreover, whether the ablated area was consistent over time and at different staining times was investigated. In addition, the presumed reversible electroporation (RE) area was formed surrounding the IRE-ablated area. Overall, TTC staining can provide a more rapid and accurate electroporated area evaluation. | Jeong, Seung; Kim, Hongbae; Park, Junhyung; Kim, Ki Woo; Sim, Sung Bo; Chung, Jong Hoon | Seoul Natl Univ, Dept Biosyst & Biomat Sci & Engn, Seoul 08826, South Korea; Kyungpook Natl Univ, Dept Ecol & Environm Syst, Sangju 37224, South Korea; Catholic Univ Korea, Dept Thorac & Cardiovasc Surg, Bucheon 14647, South Korea; Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul 08826, South Korea | Kim, Ki Woo/AAC-5623-2022 | 57221705277; 57191717781; 57202928407; 57201369889; 57206290600; 57207096830 | kiwoo@knu.ac.kr;sungbo@catholic.ac.kr;jchung@snu.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 11 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2021 | 4.997 | 25.0 | 0.65 | 2025-07-30 | 14 | 16 | IRREVERSIBLE ELECTROPORATION; ABLATION; VIABILITY | Cell Membrane; Cell Membrane Permeability; Coloring Agents; Electric Conductivity; Electroporation; Melanins; Microscopy, Electron, Transmission; Models, Biological; Plant Tubers; Solanum tuberosum; Tetrazolium Salts; coloring agent; melanin; tetrazolium; triphenyltetrazolium; biological model; cell membrane; cell membrane permeability; electric conductivity; electroporation; metabolism; plant tuber; potato; procedures; transmission electron microscopy | English | 2021 | 2021-10-14 | 10.1038/s41598-021-99987-2 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Extracellular vesicles from dHL-60 cells as delivery vehicles for diverse therapeutics | Extracellular vesicles (EVs) are membrane-derived heterogeneous vesicles that mediate intercellular communications. They have recently been considered as ideal vehicles for drug-delivery systems, and immune cells are suggested as a potential source for drug-loaded EVs. In this study, we investigated the possibility of neutrophils as a source for drug-loaded EVs. Neutrophil-like differentiated human promyelocytic leukemia cells (dHL-60) produced massive amounts of EVs within 1 h. The dHL-60 cells are also easily loaded with various cargoes such as antibiotics (penicillin), anticancer drug (paclitaxel), chemoattractant (MCP-1), miRNA, and Cas9. The EVs derived from the dHL-60 cells showed efficient incorporation of these cargoes and significant effector functions, such as bactericidal activity, monocyte chemotaxis, and macrophage polarization. Our results suggest that neutrophils or neutrophil-like promyelocytic cells could be an attractive source for drug-delivery EVs. | Kim, Jun-Kyu; Youn, Young-Jin; Lee, Yu-Bin; Kim, Sun-Hwa; Song, Dong-Keun; Shin, Minsang; Jin, Hee Kyung; Bae, Jae-sung; Shrestha, Sanjeeb; Hong, Chang-Won | Kyungpook Natl Univ, Sch Med, Dept Physiol, 680 Gukchaebosang Ro, Daegu 41944, South Korea; Hallym Univ, Coll Med, Dept Pharmacol, Chunchon 24252, South Korea; Kyungpook Natl Univ, Sch Med, Dept Microbiol, Daegu 41944, South Korea; Kyungpook Natl Univ, Coll Vet Med, Dept Lab Anim Med, Daegu 41944, South Korea; Kyungpook Natl Univ, KNU Alzheimers Dis Res Inst, Daegu 41566, South Korea | ; Bae, Jae-sung/AAM-8663-2021; Youn, Young Jin/KQU-0973-2024; Kim, Young/T-8521-2019 | 57192897853; 57195676656; 57219808197; 57219815385; 7402443736; 7401536650; 8088145800; 35209510400; 57059866700; 55567018400 | sanjeebs2012@gmail.com;cwhong@knu.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 11 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2021 | 4.997 | 25.0 | 0.54 | 2025-07-30 | 11 | 11 | NEUTROPHIL-LIKE HL-60; TRANS-RETINOIC ACID; APOPTOSIS; EXOSOMES; ECTOSOMES; SIRNA; HL60 | Anti-Bacterial Agents; Antineoplastic Agents; Cell Communication; Cell Differentiation; Cells, Cultured; Chemokine CCL2; Drug Delivery Systems; Extracellular Vesicles; Granulocyte Precursor Cells; Humans; Neutrophils; Paclitaxel; Penicillins; antiinfective agent; antineoplastic agent; CCL2 protein, human; monocyte chemotactic protein 1; paclitaxel; penicillin derivative; cell communication; cell culture; cell differentiation; cytology; drug delivery system; exosome; granulocyte precursor; human; neutrophil | English | 2021 | 2021-04-15 | 10.1038/s41598-021-87891-8 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Eye-blink artifact removal from single channel EEG with k-means and SSA | In recent years, the usage of portable electroencephalogram (EEG) devices are becoming popular for both clinical and non-clinical applications. In order to provide more comfort to the subject and measure the EEG signals for several hours, these devices usually consists of fewer EEG channels or even with a single EEG channel. However, electrooculogram (EOG) signal, also known as eye-blink artifact, produced by involuntary movement of eyelids, always contaminate the EEG signals. Very few techniques are available to remove these artifacts from single channel EEG and most of these techniques modify the uncontaminated regions of the EEG signal. In this paper, we developed a new framework that combines unsupervised machine learning algorithm (k-means) and singular spectrum analysis (SSA) technique to remove eye blink artifact without modifying actual EEG signal. The novelty of the work lies in the extraction of the eye-blink artifact based on the time-domain features of the EEG signal and the unsupervised machine learning algorithm. The extracted eye-blink artifact is further processed by the SSA method and finally subtracted from the contaminated single channel EEG signal to obtain the corrected EEG signal. Results with synthetic and real EEG signals demonstrate the superiority of the proposed method over the existing methods. Moreover, the frequency based measures [the power spectrum ratio (Gamma) and the mean absolute error (MAE)] also show that the proposed method does not modify the uncontaminated regions of the EEG signal while removing the eye-blink artifact. | Maddirala, Ajay Kumar; Veluvolu, Kalyana C. | Kyungpook Natl Univ, Sch Elect Engn, Daegu 41566, South Korea; Kyungpook Natl Univ, Sch Elect & Elect Engn, Daegu 41566, South Korea | Maddirala, Ajay Kumar/AAZ-6379-2020; Veluvolu, Kalyana/C-6366-2011 | 56638638000; 8703318200 | veluvolu@ee.knu.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 11 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2021 | 4.997 | 25.0 | 2.43 | 2025-07-30 | 36 | 51 | EMPIRICAL-MODE DECOMPOSITION; SIGNALS; ELECTROENCEPHALOGRAM; CLASSIFICATION; SEPARATION | Artifacts; Blinking; Brain; Electroencephalography; Electrooculography; Humans; Signal Processing, Computer-Assisted; algorithm; article; artifact; electroencephalogram; extraction; power spectrum; spectroscopy; unsupervised machine learning; artifact; blinking; brain; electroencephalography; electrooculography; human; physiology; signal processing | English | 2021 | 2021-05-26 | 10.1038/s41598-021-90437-7 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Genetic diversity analysis of Korean peanut germplasm using 48 K SNPs 'AxiomAᵣₐcₕᵢₛ' Array and its application for cultivar differentiation | Cultivated peanut (Arachis hypogaea) is one of the important legume oilseed crops. Cultivated peanut has a narrow genetic base. Therefore, it is necessary to widen its genetic base and diversity for additional use. The objective of the present study was to assess the genetic diversity and population structure of 96 peanut genotypes with 9478 high-resolution SNPs identified from a 48 K 'AxiomAᵣₐcₕᵢₛ' SNP array. Korean set genotypes were also compared with a mini-core of US genotypes. These sets of genotypes were used for genetic diversity analysis. Model-based structure analysis at K = 2 indicated the presence of two subpopulations in both sets of genotypes. Phylogenetic and PCA analysis clustered these genotypes into two major groups. However, clear genotype distribution was not observed for categories of subspecies, botanical variety, or origin. The analysis also revealed that current Korean genetic resources lacked variability compared to US mini-core genotypes. These results suggest that Korean genetic resources need to be expanded by creating new allele combinations and widening the genetic pool to offer new genetic variations for Korean peanut improvement programs. High-quality SNP data generated in this study could be used for identifying varietal contaminant, QTL, and genes associated with desirable traits by performing mapping, genome-wide association studies. | Nabi, Rizwana Begum Syed; Cho, Kwang-Soo; Tayade, Rupesh; Oh, Ki Won; Lee, Myoung Hee; Kim, Jung In; Kim, Sungup; Pae, Suk-Bok; Oh, Eunyoung | Rural Dev Adm, Natl Inst Crop Sci, Dept Southern Area Crop Sci, Miryang 50424, South Korea; Kyungpook Natl Univ, Sch Appl Biosci, Lab Plant Breeding, Daegu 41566, South Korea | Lee, Daesu/G-6407-2011; Kim, Kyu/E-7814-2012; Tayade, Rupesh/AAM-9652-2021 | 57200232212; 55722770800; 57191753234; 57209582322; 56142671500; 57204539161; 56016191300; 37047538100; 57204882790 | lavondy10@korea.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 11 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2021 | 4.997 | 25.0 | 0.43 | 2025-07-30 | 10 | 10 | LARGE-SCALE DEVELOPMENT; POPULATION-STRUCTURE; GENOTYPING ARRAY; MARKER ASSAYS; GENOME; DURANENSIS; HYPOGAEA; EXTRACT | Analysis of Variance; Arachis; Crops, Agricultural; Genes, Plant; Genetic Markers; Genetic Variation; Phylogeny; Polymorphism, Single Nucleotide; Principal Component Analysis; Species Specificity; article; cultivar; gene frequency; genetic variability; genetic variation; genome-wide association study; germplasm; nonhuman; peanut; population structure; principal component analysis; single nucleotide polymorphism array; structure analysis; subspecies; analysis of variance; Arachis; classification; crop; genetic marker; genetic variation; genetics; phylogeny; plant gene; single nucleotide polymorphism; species difference | English | 2021 | 2021-08-17 | 10.1038/s41598-021-96074-4 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Genetic variants in histone modification regions are associated with the prognosis of lung adenocarcinoma | We investigated the association between genetic variants in the histone modification regions and the prognosis of lung adenocarcinoma after curative surgery. Potentially functional SNPs were selected using integrated analysis of ChIP-seq and RNA-seq. The SNPs were analyzed in a discovery set (n = 166) and a validation set (n = 238). The associations of the SNPs with overall survival (OS) and disease-free survival (DFS) were analyzed. A total of 279 SNPs were selected for genotyping. Among these, CAPN1 rs17583C>T was significantly associated with better OS and DFS (P = 0.001 and P = 0.007, respectively), and LINC00959 rs4751162A>G was significantly associated with worse DFS (P = 0.008). Luciferase assays showed a significantly lower promoter activity of CAPN1 in the rs17583 T allele than C allele (P = 0.008), and consistently the CT + TT genotypes had significantly lower CAPN1 expression than CC genotype (P = 0.01) in clinical samples. The rs4751162 G allele had higher promoter activity of GLRX3 than A allele (P = 0.05). The motif analyses and ChIP-qPCR confirmed that the variants are located in the active promoter/enhancer regions where transcription factor binding occurs. This study showed that genetic variants in the histone modification regions could predict the prognosis of lung adenocarcinoma after surgery. | Kang, Hyo-Gyoung; Lee, Yong Hoon; Lee, Shin Yup; Choi, Jin Eun; Do, Sook Kyung; Hong, Mi Jeong; Lee, Jang Hyuck; Jeong, Ji Yun; Do, Young Woo; Lee, Eung Bae; Shin, Kyung Min; Lee, Won Kee; Ha Choi, Sun; Seo, Hye Won; Yoo, Seung Soo; Lee, Jaehee; Cha, Seung Ick; Kim, Chang Ho; Cho, Sukki; Jheon, Sanghoon; Park, Jae Yong | Kyungpook Natl Univ, Sch Med, Dept Biochem, Daegu, South Korea; Kyungpook Natl Univ, Sch Med, Cell & Matrix Res Inst, Daegu, South Korea; Kyungpook Natl Univ, Sch Med, Dept Internal Med, Daegu, South Korea; Kyungpook Natl Univ, Chilgok Hosp, Lung Canc Ctr, Daegu, South Korea; Kyungpook Natl Univ, Dept Biomed Sci, BK21 Plus KNU Biomed Convergence Program, Daegu, South Korea; Kyungpook Natl Univ, Sch Med, Dept Pathol, Daegu, South Korea; Kyungpook Natl Univ, Sch Med, Dept Thorac Surg, Daegu, South Korea; Kyungpook Natl Univ, Sch Med, Dept Radiol, Daegu, South Korea; Kyungpook Natl Univ Hosp, Med Res Collaborat Ctr, Daegu, South Korea; Kyungpook Natl Univ, Sch Med, Dept Med Informat, Daegu, South Korea; Seoul Natl Univ, Dept Thorac & Cardiovasc Surg, Sch Med, Seoul, South Korea | Lee, In-Kyu/AAR-6374-2021; Lee, Kyung-Soo/C-9016-2011; Lee, Yoojin/AAB-9799-2022; Lee, Jaehee/S-1697-2018 | 8573181300; 57199022948; 49863712700; 37107028100; 56333658000; 55613917100; 57161223200; 57205472984; 57189600352; 55664096100; 7402410737; 22953484700; 57199723585; 55612130200; 56479781600; 13805476000; 35227126400; 7409873555; 23993782100; 35588946900; 58360293800 | shinyup@knu.ac.kr;jaeyong@knu.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 11 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2021 | 4.997 | 25.0 | 0.22 | 2025-07-30 | 5 | 4 | CANCER; EXPRESSION; CALPAIN; EPIGENETICS; PACLITAXEL; STRINGTIE; BINDING; GROWTH; ALLELE; HISAT | Adenocarcinoma of Lung; Biomarkers, Tumor; Calpain; Carrier Proteins; Female; Gene Expression Regulation, Neoplastic; Histones; Humans; Lung Neoplasms; Male; Middle Aged; Polymorphism, Single Nucleotide; Prognosis; RNA, Long Noncoding; Survival Rate; calpain; CAPN1 protein, human; carrier protein; GLRX3 protein, human; histone; long untranslated RNA; tumor marker; chemistry; female; gene expression regulation; genetics; human; lung adenocarcinoma; lung tumor; male; metabolism; middle aged; pathology; prognosis; single nucleotide polymorphism; survival rate | English | 2021 | 2021-11-02 | 10.1038/s41598-021-00909-z | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Genome insight and description of antibiotic producing Massilia antibiotica sp. nov., isolated from oil-contaminated soil | An ivory-coloured, motile, Gram-stain-negative bacterium, designated TW-1(T) was isolated from oil-contaminated experimental soil in Kyonggi University. The phylogenetic analysis based on 16S rRNA gene sequence revealed, strain TW-1(T) formed a lineage within the family Oxalobacteraceae and clustered as members of the genus Massilia. The closest members were M. pinisoli T33(T) (98.8% sequence similarity), M. putida 6NM-7(T) (98.6%), M. arvi THG-RS2O(T) (98.5%), M. phosphatilytica 12-OD1(T) (98.3%) and M. niastensis 5516S-1(T) (98.2%). The sole respiratory quinone is ubiquinone-8. The major cellular fatty acids are hexadeconic acid, cis-9, methylenehexadeconic acid, summed feature 3 and summed feature 8. The major polar lipids are phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C content of the type strain is 66.3%. The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (dDDH) relatedness values between strain TW-1(T) and closest members were below the threshold value for species demarcation. The genome size is 7,051,197 bp along with 46 contigs and 5,977 protein-coding genes. The genome showed 5 putative biosynthetic gene clusters (BGCs) that are responsible for different secondary metabolites. Cluster 2 showed thiopeptide BGC with no known cluster blast, indicating TW-1(T) might produce novel antimicrobial agent. The antimicrobial assessment also showed that strain TW-1(T) possessed inhibitory activity against Gram-negative pathogens (Escherichia coli and Pseudomonas aeruginosa). This is the first report of the species in the genus Massilia which produces antimicrobial compounds. Based on the polyphasic study, strain TW-1(T) represents novel species in the genus Massilia, for which the name Massilia antibiotica sp. nov. is proposed. The type strain is TW-1(T) (=KACC 21627(T)=NBRC 114363(T)). | Dahal, Ram Hari; Chaudhary, Dhiraj Kumar; Kim, Jaisoo | Kyonggi Univ, Dept Life Sci, Coll Nat Sci, Suwon 16227, Kyonggi Do, South Korea; Kyungpook Natl Univ, Sch Med, Dept Microbiol, Daegu 41944, South Korea; Korea Univ, Dept Environm Engn, Sejong Campus, Sejong City 30019, South Korea | Chaudhary, Dhiraj/S-7772-2016; Dahal, Ram Hari/H-8673-2016 | 57110097800; 57191257432; 8718834500 | jkimtamu@kgu.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 11 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2021 | 4.997 | 25.0 | 0.92 | 2025-07-30 | 22 | 24 | Anti-Bacterial Agents; Genes, Bacterial; Genome, Bacterial; Genomics; Humans; Microbial Sensitivity Tests; Multigene Family; Oxalobacteraceae; Phylogeny; RNA, Ribosomal, 16S; Soil Microbiology; antiinfective agent; RNA 16S; bacterial gene; bacterial genome; biosynthesis; classification; genetics; genomics; human; isolation and purification; metabolism; microbial sensitivity test; microbiology; multigene family; Oxalobacteraceae; phylogeny; procedures | English | 2021 | 2021-03-23 | 10.1038/s41598-021-86232-z | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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